Overview

Our high-purity, 100% chemically synthesized CRISPR/Cas9 sgRNA deliver unparalleled efficiency, specificity, and consistency, enabling reliable gene editing in research and therapeutic development.

Why GenScript?

50,000+

gRNAs/year

4

Business Days

100%

On-time delivery

Pricing

Quantity 2 nmol 4 nmol 10 nmol 50 nmol 100 nmol
EasyEdit sgRNA (Desalt) $79 $149 $299 $839 $1299
SafeEdit sgRNA (HPLC) $119 $209 $459 $1299 $1899

Don’t Miss Our ONLINE EXCLUSIVE OFFER! Get 25% OFF ALL Cas9 sgRNA ONLINE ORDERS

Use Code ONLINE25 at checkout
Offer ends June 30th, 2025. Terms apply.

Need help from an expert?

Need help with your sgRNA design?

Access our intelligent design tools below to design and order Cas9 sgRNA (and DNA templates, if needed) for your CRISPR/Cas knockout or HDR knock-in experiments:

Already have your sgRNA design? Use our simple order form to order or get a quote:

Advantages

EasyEdit

Synthetic sgRNAs with modifications for reliable gene editing
1
SafeEdit

HPLC purified for improved performance
2
cGMP/GMP-like

 Supporting IND filing and clinical trials
3

EasyEdit

icon-trumpetSynthetic EasyEdit sgRNAs now starting at only $79/2nmol!

✔ Option to deliver in 96-well plates supporting high throughput screening

Most economical solution icon

Most economical solution

Ideal for early research & discovery
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High editing efficiency

Regardless of cell type
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Flexible at scale

1 to 1000+ sgRNAs 2 to 100+ nmol

EasyEdit
The easy yet effective one-step solution
Starting at $79/2nmol!

HPLC purified sgRNAs minimize off-targeting and cytotoxicity

✔ >90% purity guaranteed

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Minimal impact for
cell viability

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Reduced off-targeting from
truncated guides

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Ideal for primary cells
& stem cells

cGMP/GMP-like
Supporting IND filing and clinical trials

GenScript now offers full cGMP and INDEdit sgRNA

✔ Supporting IND filing and clinical trials

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State-of-the-art production facility

Clean suite with class A isolator in a class C background

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Comprehensive QA/QC

Proprietary NGS method to verify sgRNA identity

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Trusted by scientific partners globally

Successfully delivered 130+ cGMP batches

Science Never Stops – Neither Do We!

Support seamless transition every step of the way

With a robust global infrastructure, financial resilience, and years of CRISPR expertise, we ensure your science never skips a beat. Ready for an Upgrade? Our dedicated Transition Support Team is here to assist every step of the way!

Service Details

Service EasyEdit sgRNA SafeEdit sgRNA
Length 97-103 nt
Default Modifications 2’-O-methyl and phosphorothioate at 3’ and 5’ ends
Purification Desalt HPLC
Quantity 2-100 nmol
QC Reports COA and MS reports COA, MS, and HPLC reports
Delivery Time Starting from 7 BDs Starting from 9 BDs

Catalog Products

Cat. No. Product Name Quantity Price Order
KIOK3
Ultra eSpCas9
$199.00
 
KIOK4
Ultra WTSpCas9
$139.00
 
RC00001
RC00001
$150.00 0.1 mg
 
RP-A00018
eSpCas9 mRNA(Cap1, m1Ψ)
$300.00 0.2 mg
 
RP-A00019
GenLNP-A01-eSpCas9 mRNA(m1Ψ)-TRAC sgRNA
$300.00 0.2 mg
 
RP-A00020
GenLNP-S01-eSpCas9 mRNA(m1Ψ)-TRAC sgRNA
$300.00 0.2 mg
 
RP-A00047
saCas9 mRNA (Cap1, m1Ψ)
$300.00 0.2 mg
 
Z03622
GenCRISPR™ Ultra eSpCas9-2NLS-Research
$765.00
 

Looking for guides for other editing systems?

Prime editing guide RNA (pegRNA) Synthesis Service
Base editing guide RNA Synthesis

97-140 nt, enabling both conventional + transformer base editing

Learn More
Prime editing guide RNA (pegRNA) Synthesis Service
Prime editing guide RNA Synthesis

Up to 266 nt- the longest from any vendor- with editing efficiency of over 60%

Learn More
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CRISPR/Cas12a guide RNA Synthesis

40 to 49 nt, with superior quality and editing efficiency up to 98%

Learn More

GenScript can also synthesize desalt and HPLC purified guide RNA compatible with saCas9, Cas12f/CasMINI, Cas13, and other Cas nuclease variants.

Click here to enter your guide sequence (target sequence + scaffold sequence) and order online.

Testimonials

Case Studies

Resources

CRISPR/Cas9 gRNA Vector
RNP user manual

A guide on how to use CRISPR RNP for targeted genome editing.

Free Download
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CRISPR Knock-in Comprehensive Guide

Step-by-step instructions for your CRISPR knock-in experiments.

Free Download
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GenCRISPR gRNA & HDR Template Design Tools

For knockout and knock-in designs, with off-target analysis.

Design Now

FAQs

  • Technical questions

  • Design tool questions

  • Experiment questions

  • How many sgRNA or crRNA sequences are needed for targeted knock-out?

    A minimum of three (3) crRNA sequences are recommended to ensure knock-out and experimental accuracy. Independently obtained knock-out mutants provide redundancy to safeguard against any hidden off-target effects.

  • What are the advantages of using synthetic sgRNA for gene editing compared to the traditional plasmid- or virus-based gRNA delivery methods?

    Unlike the traditional plasmid or lentiviral delivery methods, CRISPR/Cas9 ribonucleoprotein (RNP) are delivered as intact complexes and do not require cellular expression. This method thus has many advantages:

    • Faster manufacturing: Synthetic sgRNA can be made in as little as three (3) business days using chemical synthesis and arrives ready to use- compared to a week or more of lab work required to produce gRNA via plasmid or lentiviral delivery.
    • DNA-free: Avoiding the delivery of foreign DNA into the cell eliminates any opportunity for transgene integration into the cell genome.
    • Detectable at high levels shortly after transfection: Editing activities can take place more quickly, as synthetic sgRNA doesn't need to be transcribed in the cell.
    • Quickly cleared from the cell for less off-target effects: The Cas-sgRNA RNP delivery vehicle is expressed transiently and degrades quickly, limiting the potential for any off-target editing.
    • Highly efficient even in hard-to-transfect cells: Experimental results have demonstrated high editing efficiency using synthetic sgRNA, regardless of the cell type used.
    • High packaging capacity: Our synthetic sgRNA can accommodate a length of up to 200nt.
    • Low immunogenicity (ideal for in vivo studies): Synthetic sgRNA consistently demonstrates lower immunogenicity and toxicity in edited cells compared to gRNA produced via plasmid or lentiviral delivery.
  • What is the difference between using synthetic sgRNA versus using a crRNA:tracrRNA duplex?

    When using sgRNA, there is no need for a crRNA:tracrRNA annealing step prior to use. More importantly, several studies have showed that sgRNA has better stability than crRNA:tracrRNA when duplexed with Cas9, thus leading to higher editing efficiency.1,2

    1. Hendel, et al., Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nat. Biotechnol., 33 (2015) 985-989

    2. Ryan et al., Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs. Nucleic Acids Research, 46 (2018) 2: 792–803

  • What is the advantage of synthetic sgRNA compared to in vitro-transcribed (IVT) sgRNA?

    Synthesis of 100 nt long sgRNAs was traditionally possible through in vitro transcription (IVT) using phage RNA polymerase. These in vitro-transcribed sgRNAs contain a 5’-triphosphate, which was thought to trigger immune response in many cell types. A recent study showed that sgRNAs with 5’-triphosphate modifications produced through in vitro transcription can indeed induce innate immune responses and lead to cytotoxicity in human and murine cells. However, chemically synthesized sgRNAs without the 5’-triphosphate modifications (such as GenCRISPR Synthetic sgRNAs) demonstrated much better editing efficiency in cells, thus supporting that chemically synthesized sgRNAs are the most ideal reagent for CRISPR genome editing currently available.3

    3. Kim et al. CRISPR RNAs trigger innate immune responses in human cells. Genome Res. 2018. 28: 367-373.

  • Why should I choose modified sgRNA versus unmodified versions?

    Our modified sgRNA has 2’-O-methyl and phosphorothioate modifications at the first three 5’ and 3’ terminal RNA residues. 2′ O-Methyl oligo modification is best characterized as an RNA analog which offers stability against hydrolysis and nucleases. The phosphorothioate (PS) modification renders the internucleotide linkage more resistant to nuclease degradation.

  • What container and delivery formats are available?

    Our sgRNA can be delivered in either single tubes or 96-well plates, formatted as dry powder or suspended in TE buffer (pH 8.0, 100 µM) or nuclease-free water (100 µM).

  • What is the maximum sgRNA length that you can chemically synthesize?

    We can chemically synthesize sgRNA up to 266 nt in length.

  • What is the maximum quantity of sgRNA that can be ordered? Do you offer arrayed sgRNA libraries?

    We can deliver chemically synthesized sgRNAs in an arrayed format, which is delivered in a 96-well plate. Each well contains custom-designed sgRNA targeting one gene of interest.

  • What grades of sgRNA do you have? Do you have GMP? Which type should I choose?

    We offer sgRNA in grades ranging from RUO to cGMP:

    EasyEdit sgRNA is purified by an optimized desalt method. Fast delivery and competitive pricing makes it ideal for screening or early stages of research.

    SafeEdit sgRNA is purified by HPLC, resulting in a guaranteed minimum of 90% purity. This option is ideal for the validation stage of development.

    For more information on our cGMP sgRNA, visit this page.

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with GenScript CRISPR Experts

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