Production of numerous base editors and prime editors
Base editors and prime editors are gaining more attention in gene editing era.
However, their complex structures, including multiple protein and RNA components, make them
challenging to express and purify.
At GenScript, we have successfully produced numerous base
editors and
prime editors both in high purity and decent yield.
Adenine base editor (ABE)
Cytosine base editor (CBE)
Prime editor (PE)
New! Off-the-shelf BE and PE are available
here
Type |
ABE (TadA_nCas9) |
CBE (APOBEC_nCas9) |
PE (Cas9_RT) |
Purification procedure
|
- Ni columna
- TEV protease cleavage
- Ni column
- Superdex 200 column
|
- Ni column
- Q sepharose column
- Superdex 200 column
|
- Ni column
- Superdex 200
- Ni column
|
Purity |
>90%, by SDS-PAGE under reducing conditions.
|
>90%, by SDS-PAGE under reducing conditions.
|
>90%, by SDS-PAGE under reducing conditions.
|
Yield |
1.6mg/L |
22.03mg/L |
12.3mg/L
|