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Yield up to gram, starting at 2 weeks
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Home » Protein Expression
Protein expression is almost universally used by research groups for a variety of purposes. There are three main expression systems, Bacteria, Baculovirus/insect, and Mammalian cells, available today giving researchers enough choice to find the best system for their specific application. As a leading provider of a comprehensive suite of recombinant protein and antibody services across different expression systems, GenScript seeks to partner with scientists worldwide to accelerate scientific discovery. Our proprietary GenSmart™ Codon Optimization and newly launched TurboCHO™ expression platform ensure the delivery of high-yield and purity products in a flexible, cost-effective manner in the least amount of time. Our expert scientists and dedicated technical account managers are invested in helping to maximize the success of your research projects and will guide you every step of the way.
To better align with the essential requirement of quality management for the development and manufacture of Antibody and Protein used for IVD medical devices, GenScript has completed our registration to ISO 13485:2016 standard.
NEWLY Launched TurboCHOTM System
Learn MoreDesigned for high-throughput screening, as fast as 5 BDs
Learn MoreYield up to gram, starting at 2 weeks
Learn MoreYield up to kg, from 6-7 weeks
Learn MoreThe features of a target protein inform which expression system is best suited for the expression of that protein, and it is important to identify the correct system for protein expression based on these criteria. These specific features include sequence and structure as well as activity, function, folding, and post-translational modifications (PTMs).
Bacterium | Insect | Mammalian | |||||
Prokaryotic protein expression | +++ | + | + | ||||
Eukaryotic protein without PTM | +++ | +++ | +++ | ||||
Eukaryotic protein with PTM | - | ++ | +++ | ||||
PTM, folding | + | +++ | +++ | ||||
PTM, glycosylation | - | ++ | +++ | ||||
PTM, phosphorylation | - | ++ | +++ | ||||
Turnaround time | Short | Long | Long | ||||
Cost | Low | Middle | Middle | ||||
Yield (mg/L) | Range from 1-5 mg/l with an average level 3 mg/l | Range from 0.2-50 mg/l with an average level 1 mg/l | Range from 1-1000 mg/l with an average level 50 mg/l for antibody, 3 mg/l for protein | ||||
Recommended applications | Antigen protein, Protein standards, Functional proteins | Proteins with glycosylation, Assay standards, Secreted form | Functional study, PTM study, Assay standards, Characterization | ||||
Learn More | Learn More | Learn More |
E.coli is one of the most commonly used protein expression systems and protein expression is usually induced using a DNA plasmid expression vector. This bacterium can be grown easily and inexpensively under standard laboratory conditions and expression using this system is relatively simple, fast, and inexpensive. If a target protein originates from a prokaryotic system, E.coli is an easy choice. If, however, the protein is of eukaryotic origin, the PTMs cannot be guaranteed in this system and another system may be preferable. In the case that PTMs are not important in the target protein, E.coli can still be a good choice for even eukaryotic protein expression.
Baculovirus/Insect expression system allows for expression of high quality glycosylated proteins that cannot be expressed using E.coli or yeast. Insect cells are a higher eukaryotic system than yeast and are therefore better able to carry out more complex PTMs. They also have superior machinery for folding mammalian proteins and therefore give the best chance of obtaining soluble protein when a protein of mammalian origin is expressed.
Mammalian cells are more complex than bacterial cells and have the most sophisticated protein production capabilities including mechanisms to assist protein folding into the proper conformation like chaperones and modifications (glycosylation, methylation, phosphorylation etc.). For a protein with mammalian origin, the activity can be related not only to the primary structure (sequence), but also to the conformation and modifications, while other systems, especially when distantly related, cannot accomplish all the modifications found naturally in mammalian cells. This is a reason why many researchers believe the activity of mammalian proteins is best when expressed in a mammalian cell. GenScript employs genetically modified CHO and HEK293 cells together with elaborately designed expression vectors to give high yield and quality protein production, which has been proven by hundreds of successful projects.
GenScript has been on of our most trusted resources for our lab for many years now. We've outsource countless projects and almost always have mulitple ongoing projects in progress. From the top down, the account managers, technical team and promptness of service is always consistent and we wouldn't think of trusting any one else with our projects. We've never had anything but the most positive of experiences with GenScript. They should be commended for their consistenty and reliability.
"I can attest to a very positive experience in working before protein expression scientists at GenScript. We tasked the GenScript team with challenging projects for the production of engineered proteins. We experienced frequent communication on progress, transparent data sharing, and open discussions on results and troubleshooting. The GenScript team demonstrated a high level of commitment both timelines and quality product delivery. I would highly recommend them for scientists seeking to advance their projects in a collaborative way."
"I have been delighted to use this outstanding service for the past five years. My experiences with the protein expression services have been exceptional, especially when working with mutated variants. The results have always been reproducible and of the highest quality, leading to excellent outcomes that were published in prestigious journals"
"GenScript has been very helpful for years. I consider them part of my lab. Rather than spending weeks and months expressing proteins, I get turn around on orders in a few weeks, and am never disappointed."
"I've had the privilege of collaborating with various service providers, but GenScript has consistently stood out in terms of reliability, quality, and innovation. Over the past few years, GenScript has become an indispensable partner in our research endeavors. Moreover, their custom protein services have been a game-changer. The purity and functionality of the proteins we’ve ordered have always met, if not exceeded, our expectations. This level of quality is crucial for the reproducibility of our experiments and the integrity of our results."
"Excellent quality and fast services, with a vast offer of molecular biology and protein/antibody products. The technical support is particularly helpful in finding the best solution for the specific experimental needs. Pricing is competitive and turnaround time is usually within the planned delivery date."
"GenScript provides fast, professional protein synthesis services at very reasonable prices. By making it cost-effective to outsource protein production, GenScript has made it possible for my lab to focus on our own area of expertise and get more research done. The detailed planning, updates, and reports that GenScript provides all of the quality control that one could ask for. I strongly recommend GenScript's protein production service. "
A handbook chapter with detailed services for recombinant protein expression and cell line development platform
Free Download Free DownloadA handbook chapter with detailed protocols and tips for rAb expression.
Free Download Free DownloadA white paper with technical advice for optimizing your system for rAb expression.
Free Download Free DownloadA white paper with technical advice for optimizing your system for rAb expression.
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