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saCas9 mRNA (Cap1, m1Ψ)

$300.00
RP-A00047-0.2

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Description

The saCas9 mRNA expresses an wild type Staphylococcus aureus cas9 (saCas9) enzymatic protein. After efficient delivery into cells or animals, saCas9 mRNA can translate into protein function for gene editing in cells.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
产品浓度 1mg/mL
Full mRNA length 3534nt
Full mRNA Molecular Weight 1.14 X 10^6 Da
储存条件 Store at -20°C for short term (<3 months), store at -80°C for long term.
Storage buffer 1mM Sodium citrate, pH6.5
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Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5
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Applications
Transfect a total of 0.5-1.5 µg of saCas9 mRNA and sgRNA per well (recommended molar ratio 1:10), using a transfection reagent, electroporation, or LNP encapsulation in a 24-well plate (~2 × 10⁵ cells/well). Assess genome editing efficiency 48-72 hours post-transfection via sequencing and ICE analysis.
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.