Cas12a (cpf1) crRNA Synthesis Service

Advantages

Superior Quality

Precise molecular weight, high purity,
excellent batch-to-batch consistency, RUO to cGMP

Proven Efficiency

High gene knockout/knock-in efficiency
verified in multiple cell lines & loci

Flexible Solutions

Desalt and HPLC Grade
None or complimentary PS-2′-Ome modifications

Application

KO/KI Basic Research

Species rich in AT-genome, etc.

Gene & Cell Therapy

High fidelity with low off-target effects

Diagnostic Tools

DETECTR technology used for
the detection of viruses, tumor , etc.

Services

Modification Comments>>

• 2’Ome (the first 3 bases) at 5’ end of crRNA was found compromising Cpf1 activity (McMahon et al., 2018; Rozners, 2022; Ha et al, 2020)
• 2’Ome (the last 3 bases) at 3’ end was well tolerated, showing no negative impact on editing activity (McMahon et al., 2018; Ha et al, 2020). A report demonstrates the modification suppressed Cas12a‘s affinity to off-target DNA while maintaining high affinity to on-target DNA (Ke et al., 2022).

Pricing

Need help from an expert to support your Cas12a crRNA request?

Case Studies

• Precise molecular weight & high purity
  

  Dealt purified crRNA:
  Precise molecular weight

  

  HPLC purified crRNA:
  Precise molecular weight

  

  HPLC purified crRNA:
  Purity up to 99.20%

• High knock-out efficiency up to 98%

  Results: Using GenScript's Cas12a crRNA, high gene knock-out efficiency can be achieved with different Cas12a enzymes：

  • HEK293T cells: AsCas12a knock-out efficiency up to 98%, LbCas12a knock-out efficiency up to 93%, MAD7 knock-out efficiency up to 94%.
  • Jurkat cells: AsCas12a knock-out efficiency up to 98%, LbCas12a knock-out efficiency up to 98%, MAD7 knock-out efficiency up to 97%.

  

  
• High knock-in efficiency up to 36%

  Results: Using GenScript's Cas12a crRNA, high gene knock-in efficiency can be achieved for different enzymes:

  • TRAC locus: AsCas12a knock-in efficiency up to 23%, LbCas12a knock-in efficiency up to 25%, MAD7 knock-in efficiency up to 24%.
  • B2M locus: AsCas12a knock-in efficiency up to 33%, LbCas12a knock-in efficiency up to 36%, MAD7 knock-in efficiency up to 30%.

  

  
• Low cytotoxicity, reducing confounding factors

  Results: GenScript's Cas12a crRNA does not affect the cell viability of HEK293 cells and Jurkat cells, and will not interfere with subsequent gene editing experiments.

  

  

Cas12a crRNA Design Tool

GenScript offers an easy-to-use crRNA design tool for AsCas12a and LbCas12a, which includes a variety of crRNA design parameters for your reference and selection. Try it now:

Resources

Cas12a Editing Protocol for HEK 293T/293 Cells Using the Thermo Fisher Neon® Transfection System

Free Download

Looking for guides for other editing systems?

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Prime editing guide RNA (pegRNA) Synthesis Service

110 to 231 nt- the longest synthetic guides from any supplier- with editing efficiency over 60%

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GenScript can also synthesize desalt and HPLC purified guide RNA compatible with saCas9, Cas12f/CasMINI, Cas13, and other Cas nuclease variants.

Click here to enter your guide sequence (target sequence + scaffold sequence) and order online.