Base editing guide RNA (gRNA)

Advantages

Desalt & HPLC-purified Options

Precise molecular weight, high purity, excellent batch-to-batch consistency

Proven Editing Efficiency

Editing efficiency over 90%
Modifications to enhance efficiency

One-Stop Solution

Base editor mRNA and protein available

RUO to cGMP Capability

Supporting research to IND application & clinical trials

Service Details

Catalog Products

Validate your base editing experimental system and optimize editing efficiency with our off-the-shelf base editing products:

1. The ABE8a protein was retrieved from David R Liu, et al “Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity.” Nature Biotechnology 2020; 38(7): 883-891.
2. The CBEmax mRNA sequence is originally from Walton et al “Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.” Science 2020; 368(6488): 290-296
3. The B2M sgRNA and validation primer sequences were retrieved from Gaudelli, N. W. et al “Directed evolution of adenine base editors with increased activity and therapeutic application.” Nature Biotechnology 2020; 38, 892–900.

Looking for guides for other editing systems?

Cas9 sgRNA Synthesis Service

97 to 103 nt, improving your editing efficiency at a cost-effective price

Learn More

Prime editing guide RNA (pegRNA) Synthesis Service

110 to 231 nt- the longest synthetic guides from any supplier- with editing efficiency over 60%

Learn More

Cas12a (cpf1) crRNA Synthesis Service

40 to 49 nt, with superior quality and editing efficiency up to 98%

Learn More

GenScript can also synthesize desalt and HPLC purified guide RNA compatible with saCas9, Cas12f/CasMINI, Cas13, and other Cas nuclease variants.

Click here to enter your guide sequence (target sequence + scaffold sequence) and order online.

Case Studies

• Based editing sgRNA exhibits precise molecular weight & high purity

  HPLC purified pegRNA (266 nt): Precise molecular weight

  
• 96.8% C-to-T Conversion Achieved with CBEmax mRNA

  HPLC purified pegRNA (266 nt): Precise molecular weight

  
• 97.0% A-to-G Conversion Achieved with ABE8a Protein

  HPLC purified pegRNA (266 nt): Precise molecular weight

  

Resources

Base Editing Protocol for Jurkat cells with Thermo Fisher Scientific Neon™ NxT Electroporation System

Free Download

FAQs

• What are the advantages of using base editing for gene modification compared to CRISPR-Cas9?

  Unlike CRISPR-Cas9, which creates double-strand breaks (DSBs) in DNA, base editing enables precise single-nucleotide conversions (C-to-T or A-to-G) without inducing DSBs or requiring a donor DNA template. This approach reduces the risk of unwanted insertions or deletions (indels), minimizes off-target effects, and lowers cellular stress, making it a safer and more efficient gene editing method, especially in non-dividing cells.

• What purity levels do you offer for base editing gRNA synthesis?

  We offer both desalt-purified and HPLC-purified base editing gRNA, in both non-clinical (RUO and IND-enabling) and clinical (cGMP) grades.

• What is the maximum quantity of base editing gRNA that can be ordered?

  We can deliver up to 100 nmol base editing gRNA as part of our standard offering. For other quantities, please inquire here.

• Besides base editing gRNA, what additional reagents will I need to perform base editing?

  In addition to base editing gRNA, base editing requires a base editor enzyme, delivered in either mRNA or protein form. The base editor, which consists of either a deactivated Cas protein (dCas9) or Cas9 nickase (nCas9) with either a cytidine or adenine deaminase- is guided by the gRNA to an editing window of about 4-9 nucleotides where it performs a precise C-to-T or A-to-G DNA base conversion.

  GenScript offers CBEmax mRNA (Cat# RP-A00002) and ABE8e protein (Cat# RC00010), both available off-the-shelf.

• What container and delivery formats are available for base editing gRNA?

  Our base editing gRNA can be delivered in either single tubes or 96-well plates, formatted as dry powder or suspended in TE buffer (pH 8.0, 100 µM) or nuclease-free water (100 µM).

• How should I store base editing gRNA?

  Dissolve the powdered base editing gRNA in TE buffer to a concentration of 100 pmol/µL, or other desired concentration. Divide into aliquots of at least 10 μl each and store at -20℃ for up to 6 months, avoiding repeated freeze-thaw cycles. For long-term storage, store at -80℃.