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PCR Cloning

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GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme Prototype Sequence Cut Site Overhang
SacI GAGCTC GAGCT^C
C^TCGAG		 AGCT - 3'
SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
SacNI HgiJII GRGCYC GRGCY^C
C^RCGYG		 RGCY - 3'
SalI GTCGAC G^TCGAC
CAGCT^G		 5' - AGCT
SalPI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
SanDI GGGWCCC GG^GWCCC
CCCWG^GG		 5' - CWG
SapI GCTCTTC GCTCTTCN^NNN
CGAGAAGNNNN^		 5' - NNN
SarI StuI AGGCCT AGG^CCT
TCC^GGA		 Blunt
SatI Fnu4HI GCNGC GC^NGC
CGN^CG		 5' - N
SauI CCTNAGG CC^TNAGG
GGANT^CC		 5' - ANT
Sau96I AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
Sau3239I XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
Sau3AI MboI GATC ^GATC
CTAG^		 5' - CTAG
SauBMKI NaeI GCCGGC GCC^GGC
CGG^CCG		 Blunt
SauHPI NaeI GCCGGC GCC^GGC
CGG^CCG		 Blunt
SauLPI NaeI GCCGGC GCC^GGC
CGG^CCG		 Blunt
SauLPII XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
SauMI MboI GATC ^GATC
CTAG^		 5' - CTAG
SauNI NaeI GCCGGC GCC^GGC
CGG^CCG		 Blunt
SauSI NaeI GCCGGC GCC^GGC
CGG^CCG		 Blunt
SbfI Sse8387I CCTGCAGG CCTGCA^GG
GG^ACGTCC		 TGCA - 3'
Sbi68I XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
Sbo13I NruI TCGCGA TCG^CGA
AGC^GCT		 Blunt
SbvI HaeIII GGCC GG^CC
CC^GG		 Blunt
ScaI AGTACT AGT^ACT
TCA^TGA		 Blunt
SceIII NaeI GCCGGC G^CCGGC
CGGCC^G		 5' - GGCC
SchI PleI GAGTC GAGTCNNNNN^
CTCAGNNNNN^		 Blunt
SchZI SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
SciI XhoI CTCGAG CTC^GAG
GAG^CTC		 Blunt
SciNI HhaI GCGC G^CGC
CGC^G		 5' - GC
ScrFI CCNGG CC^NGG
GGN^CC		 5' - N
SdaI Sse8387I CCTGCAGG CCTGCA^GG
GG^ACGTCC		 TGCA - 3'
SdiI SfiI GGCCNNNNNGGCC GGCCNNNN^NGGCC
CCGGN^NNNNCCGG		 NNN - 3'
SduI GDGCHC GDGCH^C
C^DCGHG		 DGCH - 3'
SecI CCNNGG C^CNNGG
GGNNC^C		 5' - GNNC
SelI FnuDII CGCG ^CGCG
GCGC^		 5' - GCGC
SenPT16I XmaIII CGGCCG C^GGCCG
GCCGG^C		 5' - CCGG
SenPT14bI SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
SepI AvaIII ATGCAT ATGCA^T
T^ACGTA		 TGCA - 3'
SexAI ACCWGGT A^CCWGGT
TGGWCC^A		 5' - GGWCC
SexBI SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
SexCI SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
SfaI HaeIII GGCC GG^CC
CC^GG		 Blunt
SfaNI GCATC GCATCNNNNN^NNNN
CGTAGNNNNNNNNN^		 5' - NNNN
SfcI SfeI CTRYAG C^TRYAG
GARYT^C		 5' - ARYT
SfeI CTRYAG C^TRYAG
GARYT^C		 5' - ARYT
SfiI GGCCNNNNNGGCC GGCCNNNN^NGGCC
CCGGN^NNNNCCGG		 NNN - 3'
SflI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
SfoI NarI GGCGCC GGC^GCC
CCG^CGG		 Blunt
Sfr274I XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
Sfr303I SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
SfuI AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
SgfI GCGATCGC GCGAT^CGC
CGC^TAGCG		 AT - 3'
SgrAI CRCCGGYG CR^CCGGYG
GRGGCC^YC		 5' - GGCC
SgrBI SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
SimI GGGTC GG^GTC
CCCAG^		 5' - CAG
SinI AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
SlaI XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
SleI EcoRII CCWGG ^CCWGG
GGWCC^		 5' - GGWCC
Slu1777I NaeI GCCGGC GCC^GGC
CGG^CCG		 Blunt
SmaI CCCGGG CCC^GGG
GGG^CCC		 Blunt
SmiI SwaI ATTTAAAT ATTT^AAAT
TAAA^TTTA		 Blunt
SmiMI MslI CAYNNNNRTG CAYNN^NNRTG
GTYNN^NNRAC		 Blunt
SmlI CTYRAG C^TYRAG
GAYRT^C		 5' - AYRT
SmuI FauI CCCGC CCCGCNNNN^NN
GGGCGNNNNNN^		 5' - NN
SmuEI AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
SnaBI TACGTA TAC^GTA
ATG^CAT		 Blunt
SniI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
SnoI ApaLI GTGCAC G^TGCAC
CACGT^G		 5' - ACGT
SolI BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
Sol10179I XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
SpaHI SphI GCATGC GCATG^C
C^GTACG		 CATG - 3'
SpeI ACTAGT A^CTAGT
TGATC^A		 5' - GATC
SphI GCATGC GCATG^C
C^GTACG		 CATG - 3'
SplI CGTACG C^GTACG
GCATG^C		 5' - CATG
SpmI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
SpoI NruI TCGCGA TCG^CGA
AGC^GCT		 Blunt
SpuI SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
SrfI GCCCGGGC GCCC^GGGC
CGGG^CCCG		 Blunt
SrlI NaeI GCCGGC G^CCGGC
CGGCC^G		 5' - GGCC
Srl5DI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
Srl32DII EcoRI GAATTC G^AATTC
CTTAA^G		 5' - TTAA
Srl55DI EcoRI GAATTC G^AATTC
CTTAA^G		 5' - TTAA
Srl56DI SfeI CTRYAG C^TRYAG
GARYT^C		 5' - ARYT
SruI AhaIII TTTAAA TTT^AAA
AAA^TTT		 Blunt
Sru4DI VspI ATTAAT AT^TAAT
TAAT^TA		 5' - AT
Sru30DI StuI AGGCCT AGG^CCT
TCC^GGA		 Blunt
SsbI HindIII AAGCTT A^AGCTT
TTCGA^A		 5' - TCGA
SscL1I HinfI GANTC G^ANTC
CTNA^G		 5' - TNA
Sse9I TspEI AATT ^AATT
TTAA^		 5' - TTAA
Sse232I CGCCGGCG CG^CCGGCG
GCGGCC^GC		 5' - GGCC
Sse1825I SanDI GGGWCCC GG^GWCCC
CCCWG^GG		 5' - CWG
Sse8387I CCTGCAGG CCTGCA^GG
GG^ACGTCC		 TGCA - 3'
Sse8647I AGGWCCT AG^GWCCT
TCCWG^GA		 5' - CWG
SseAI NarI GGCGCC GG^CGCC
CCGC^GG		 5' - GC
SseBI StuI AGGCCT AGG^CCT
TCC^GGA		 Blunt
SshAI SauI CCTNAGG CC^TNAGG
GGANT^CC		 5' - ANT
SsiI AciI CCGC C^CGC
GGC^G		 5' - GC
SsiAI MboI GATC ^GATC
CTAG^		 5' - CTAG
SsiBI MboI GATC ^GATC
CTAG^		 5' - CTAG
SslI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
SsoI EcoRI GAATTC G^AATTC
CTTAA^G		 5' - TTAA
SsoII ScrFI CCNGG ^CCNGG
GGNCC^		 5' - GGNCC
SspI AATATT AAT^ATT
TTA^TAA		 Blunt
Ssp1I AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
Ssp4800I Bsp1407I TGTACA T^GTACA
ACATG^T		 5' - CATG
Ssp5230I AatII GACGTC GACGT^C
C^TGCAG		 ACGT - 3'
Ssp27144I ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
SspAI EcoRII CCWGG ^CCWGG
GGWCC^		 5' - GGWCC
SspBI Bsp1407I TGTACA T^GTACA
ACATG^T		 5' - CATG
SspCI NaeI GCCGGC GCC^GGC
CGG^CCG		 Blunt
SspD5I HphI GGTGA GGTGANNNNNNNN^
CCACTNNNNNNNN^		 Blunt
SspD5II AvaIII ATGCAT ATGCA^T
T^ACGTA		 TGCA - 3'
SspRFI AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
SsrI HpaI GTTAAC GTT^AAC
CAA^TTG		 Blunt
SstI SacI GAGCTC GAGCT^C
C^TCGAG		 AGCT - 3'
SstII SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
Sst12I PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
SteI StuI AGGCCT AGG^CCT
TCC^GGA		 Blunt
SthI KpnI GGTACC G^GTACC
CCATG^G		 5' - CATG
Sth117I EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
Sth132I CCCG CCCGNNNN^NNNN
GGGCNNNNNNNN^		 5' - NNNN
Sth134I HpaII CCGG C^CGG
GGC^C		 5' - GC
Sth368I MboI GATC ^GATC
CTAG^		 5' - CTAG
StrI XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
StsI FokI GGATG GGATGNNNNNNNNNN^NNNN
CCTACNNNNNNNNNNNNNN^		 5' - NNNN
StuI AGGCCT AGG^CCT
TCC^GGA		 Blunt
StyI CCWWGG C^CWWGG
GGWWC^C		 5' - GWWC
StyD4I ScrFI CCNGG ^CCNGG
GGNCC^		 5' - GGNCC
SuaI HaeIII GGCC GG^CC
CC^GG		 Blunt
SunI SplI CGTACG C^GTACG
GCATG^C		 5' - CATG
SurI BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
SviI AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
SwaI ATTTAAAT ATTT^AAAT
TAAA^TTTA		 Blunt
SetI ASST ASST^
^TSSA		 ASST - 3'
SfaAI GCGATCGC GCGAT^CGC
CGC^TAGCG		 AT - 3'
SgrDI CGTCGACG CG^TCGACG
GCAGCT^GC		 5' - AGCT
SgsI GGCGCGCC GG^CGCGCC
CCGCGC^GG		 5' - GCGC
SmoI CTYRAG C^TYRAG
GAYRT^C		 5' - AYRT
SspDI GGCGCC G^GCGCC
CCGCG^G		 5' - CGCG