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PCR Cloning

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GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme Prototype Sequence Cut Site Overhang
LcaI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
LlaAI MboI GATC ^GATC
CTAG^		 5' - CTAG
LlaBI SfeI CTRYAG C^TRYAG
GARYT^C		 5' - ARYT
LlaCI HindIII AAGCTT A^AGCTT
TTCGA^A		 5' - TCGA
LlaG2I NheI GCTAGC G^CTAGC
CGATC^G		 5' - GATC
Lmu60I SauI CCTNAGG CC^TNAGG
GGANT^CC		 5' - ANT
LplI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
LpnI HaeII RGCGCY RGC^GCY
RCG^CGY		 Blunt
LspI AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
LweI SfaNI GCATC GCATCNNNNN^NNNN
CGTAGNNNNNNNNN^		 5' - NNNN
LguI GCTCTTCNNNN GCTCTTCN^NNN
CGA^GAAGNNNN		 CTTCN - 3'
Lsp1109I GCAGC(N)8(N)4 GCAGC(N)8(N)^4
CGTC^G(N)8(N)4		 C(N)8(N) - 3'