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PCR Cloning

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GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme Prototype Sequence Cut Site Overhang
No preference ^
^		 Blunt
NaeI GCCGGC GCC^GGC
CGG^CCG		 Blunt
NarI GGCGCC GG^CGCC
CCGC^GG		 5' - GC
NblI PvuI CGATCG CGAT^CG
GC^TAGC		 AT - 3'
NciI CauII CCSGG CC^SGG
GGS^CC		 5' - S
NcoI CCATGG C^CATGG
GGTAC^C		 5' - GTAC
NcrI BglII AGATCT A^GATCT
TCTAG^A		 5' - CTAG
NcuI MboII GAAGA GAAGANNNNNNNN^
CTTCTNNNNNNN^N		 N - 3'
NdaI NarI GGCGCC GG^CGCC
CCGC^GG		 5' - GC
NdeI CATATG CA^TATG
GTAT^AC		 5' - AT
NdeII MboI GATC ^GATC
CTAG^		 5' - CTAG
NgoAIII SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
NgoAIV NaeI GCCGGC G^CCGGC
CGGCC^G		 5' - GGCC
NgoMIV NaeI GCCGGC G^CCGGC
CGGCC^G		 5' - GGCC
NgoPII HaeIII GGCC GG^CC
CC^GG		 Blunt
NgoPIII SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
NheI GCTAGC G^CTAGC
CGATC^G		 5' - GATC
NlaII MboI GATC ^GATC
CTAG^		 5' - CTAG
NlaIII CATG CATG^
^GTAC		 CATG - 3'
NlaIV GGNNCC GGN^NCC
CCN^NGG		 Blunt
Nli3877I AvaI CYCGRG CYCGR^G
G^YGCRC		 YCGR - 3'
NmeCI MboI GATC ^GATC
CTAG^		 5' - CTAG
NmeRI PvuII CAGCTG CAG^CTG
GTC^GAC		 Blunt
NmuCI Tsp45I GTSAC ^GTSAC
CASTG^		 5' - CASTG
NopI SalI GTCGAC G^TCGAC
CAGCT^G		 5' - AGCT
NotI GCGGCCGC GC^GGCCGC
CGCCGG^CG		 5' - CCGG
NphI MboI GATC ^GATC
CTAG^		 5' - CTAG
NruI TCGCGA TCG^CGA
AGC^GCT		 Blunt
NruGI Eam1105I GACNNNNNGTC GACNNN^NNGTC
CTGNN^NNNCAG		 N - 3'
NsbI MstI TGCGCA TGC^GCA
ACG^CGT		 Blunt
NsiI AvaIII ATGCAT ATGCA^T
T^ACGTA		 TGCA - 3'
NsiCI EcoRV GATATC GAT^ATC
CTA^TAG		 Blunt
NspI RCATGY RCATG^Y
R^GTACY		 CATG - 3'
NspII SduI GDGCHC GDGCH^C
C^DCGHG		 DGCH - 3'
NspIII AvaI CYCGRG C^YCGRG
GYGCR^C		 5' - YGCR
NspIV AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
NspV AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
Nsp7121I AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
Nsp29132II BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
NspBII CMGCKG CMG^CKG
GMC^GKC		 Blunt
NspHI NspI RCATGY RCATG^Y
R^GTACY		 CATG - 3'
NspLKI HaeIII GGCC GG^CC
CC^GG		 Blunt
NspMACI BglII AGATCT A^GATCT
TCTAG^A		 5' - CTAG
NspSAI AvaI CYCGRG C^YCGRG
GYGCR^C		 5' - YGCR
NspSAII BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
NspSAIV BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
NunII NarI GGCGCC GG^CGCC
CCGC^GG		 5' - GC
NmeAIII GCCGAG GCCGAGNNNNNNNNNNNNNNNNNNNN^
CGGCTCNNNNNNNNNNNNNNNNNN^NN		 NN - 3'