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PCR Cloning

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GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme Prototype Sequence Cut Site Overhang
CacI MboI GATC ^GATC
CTAG^		 5' - CTAG
Cac8I GCNNGC GCN^NGC
CGN^NCG		 Blunt
CaiI AlwNI CAGNNNCTG CAGNNN^CTG
GTC^NNNGAC		 NNN - 3'
CauI AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
CauII CCSGG CC^SGG
GGS^CC		 5' - S
CauB3I BspMII TCCGGA T^CCGGA
AGGCC^T		 5' - GGCC
CbiI AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
CboI HpaII CCGG C^CGG
GGC^C		 5' - GC
CbrI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
CciNI NotI GCGGCCGC GC^GGCCGC
CGCCGG^CG		 5' - CCGG
CcoI NaeI GCCGGC GCC^GGC
CGG^CCG		 Blunt
CcrI XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
CcuI AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
CcyI MboI GATC ^GATC
CTAG^		 5' - CTAG
CdiI CATCG CATC^G
GTAG^C		 Blunt
CelI BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
CelII EspI GCTNAGC GC^TNAGC
CGANT^CG		 5' - ANT
CeqI EcoRV GATATC GAT^ATC
CTA^TAG		 Blunt
CflI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
CfoI HhaI GCGC GCG^C
C^GCG		 CG - 3'
CfrI YGGCCR Y^GGCCR
YCCGG^R		 5' - CCGG
Cfr6I PvuII CAGCTG CAG^CTG
GTC^GAC		 Blunt
Cfr9I SmaI CCCGGG C^CCGGG
GGGCC^C		 5' - GGCC
Cfr10I RCCGGY R^CCGGY
RGGCC^Y		 5' - GGCC
Cfr13I AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
Cfr42I SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
CfrA4I PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
CfrBI StyI CCWWGG C^CWWGG
GGWWC^C		 5' - GWWC
CfrJ4I SmaI CCCGGG CCC^GGG
GGG^CCC		 Blunt
CfuI DpnI GATC GA^TC
CT^AG		 Blunt
CfuII PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
ChaI MboI GATC GATC^
^CTAG		 GATC - 3'
CjeI CCANNNNNNGT CCANNNNNNGTNNNNNNNNNNNNNNN^
GGTNNNNNNCANNNNNNNNN^NNNNNN		 NNNNNN - 3'
CjePI CCANNNNNNNTC CCANNNNNNNTCNNNNNNNNNNNNNN^
GGTNNNNNNNAGNNNNNNNN^NNNNNN		 NNNNNN - 3'
ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
CltI HaeIII GGCC GG^CC
CC^GG		 Blunt
CpfI MboI GATC ^GATC
CTAG^		 5' - CTAG
CpoI RsrII CGGWCCG CG^GWCCG
GCCWG^GC		 5' - CWG
CscI SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
CsiAI AgeI ACCGGT A^CCGGT
TGGCC^A		 5' - GGCC
CsiBI NotI GCGGCCGC GC^GGCCGC
CGCCGG^CG		 5' - CCGG
CspI RsrII CGGWCCG CG^GWCCG
GCCWG^GC		 5' - CWG
Csp6I RsaI GTAC G^TAC
CAT^G		 5' - AT
Csp45I AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
CspAI AgeI ACCGGT A^CCGGT
TGGCC^A		 5' - GGCC
CspBI NotI GCGGCCGC GC^GGCCGC
CGCCGG^CG		 5' - CCGG
CspCI CAANNNNNGTGG CAANNNNNGTGGNNNNNNNNNNNN^
GTTNNNNNCACCNNNNNNNNNN^NN		 NN - 3'
Csp68KI AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
Csp68KII AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
Csp68KIII AvaIII ATGCAT ATGCA^T
T^ACGTA		 TGCA - 3'
Csp68KVI FnuDII CGCG CG^CG
GC^GC		 Blunt
CspKVI FnuDII CGCG CG^CG
GC^GC		 Blunt
CstI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
CstMI AAGGAG AAGGAGNNNNNNNNNNNNNNNNNNNN^
TTCCTCNNNNNNNNNNNNNNNNNN^NN		 NN - 3'
CthII EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
CviAI MboI GATC ^GATC
CTAG^		 5' - CTAG
CviAII NlaIII CATG C^ATG
GTA^C		 5' - TA
CviBI HinfI GANTC G^ANTC
CTNA^G		 5' - TNA
CviJI RGCY RG^CY
RC^GY		 Blunt
CviQI RsaI GTAC G^TAC
CAT^G		 5' - AT
CviRI TGCA TG^CA
AC^GT		 Blunt
CviRII RsaI GTAC G^TAC
CAT^G		 5' - AT
CviTI CviJI RGCY RG^CY
RC^GY		 Blunt
CvnI SauI CCTNAGG CC^TNAGG
GGANT^CC		 5' - ANT
CciI tcatga t^catga
tcatg^a		 5' - catg
CseI gacgc gacgcNNNNNN^NNNNN
gacgcNNNNNNNNNNN^		 5' - NNNNN
CviKI-1 RGCY RG^CY
RC^GY		 Blunt