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PCR Cloning

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GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme Prototype Sequence Cut Site Overhang
MabI SexAI ACCWGGT A^CCWGGT
TGGWCC^A		 5' - GGWCC
MaeI CTAG C^TAG
GAT^C		 5' - AT
MaeII ACGT A^CGT
TGC^A		 5' - GC
MaeIII GTNAC ^GTNAC
CANTG^		 5' - CANTG
MaeK81I SplI CGTACG C^GTACG
GCATG^C		 5' - CATG
MaeK81II AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
MamI BsaBI GATNNNNATC GATNN^NNATC
CTANN^NNTAG		 Blunt
MavI XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
MbiI BsrBI CCGCTC CCG^CTC
GGC^GAG		 Blunt
MboI GATC ^GATC
CTAG^		 5' - CTAG
MboII GAAGA GAAGANNNNNNNN^
CTTCTNNNNNNN^N		 N - 3'
MchI NarI GGCGCC GG^CGCC
CCGC^GG		 5' - GC
MchAI NotI GCGGCCGC GC^GGCCGC
CGCCGG^CG		 5' - CCGG
MchAII HaeIII GGCC GG^CC
CC^GG		 Blunt
McrI CGRYCG CGRY^CG
GC^RYGC		 RY - 3'
MfeI CAATTG C^AATTG
GTTAA^C		 5' - TTAA
MflI XhoII RGATCY R^GATCY
RCTAG^Y		 5' - CTAG
MfoAI HaeIII GGCC GG^CC
CC^GG		 Blunt
Mgl14481I CauII CCSGG CC^SGG
GGS^CC		 5' - S
MgoI MboI GATC ^GATC
CTAG^		 5' - CTAG
MhaAI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
MhlI SduI GDGCHC GDGCH^C
C^DCGHG		 DGCH - 3'
MkrAI MboI GATC ^GATC
CTAG^		 5' - CTAG
MlaI AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
MlaAI XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
MlsI BalI TGGCCA TGG^CCA
ACC^GGT		 Blunt
MltI AluI AGCT AG^CT
TC^GA		 Blunt
MluI ACGCGT A^CGCGT
TGCGC^A		 5' - GCGC
Mlu23I BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
Mlu31I BalI TGGCCA TGG^CCA
ACC^GGT		 Blunt
MluB2I NruI TCGCGA TCG^CGA
AGC^GCT		 Blunt
MluNI BalI TGGCCA TGG^CCA
ACC^GGT		 Blunt
MlyI PleI GAGTC GAGTCNNNNN^
CTCAGNNNNN^		 Blunt
Mly113I NarI GGCGCC GG^CGCC
CCGC^GG		 5' - GC
MmeI TCCRAC TCCRACNNNNNNNNNNNNNNNNNNNN^
AGGRTGNNNNNNNNNNNNNNNNNN^NN		 NN - 3'
MnlI CCTC CCTCNNNNNNN^
GGAGNNNNNN^N		 N - 3'
MnoI HpaII CCGG C^CGG
GGC^C		 5' - GC
Mph1103I AvaIII ATGCAT ATGCA^T
T^ACGTA		 TGCA - 3'
MroI BspMII TCCGGA T^CCGGA
AGGCC^T		 5' - GGCC
MroNI NaeI GCCGGC G^CCGGC
CGGCC^G		 5' - GGCC
MroXI XmnI GAANNNNTTC GAANN^NNTTC
CTTNN^NNAAG		 Blunt
MscI BalI TGGCCA TGG^CCA
ACC^GGT		 Blunt
MseI TTAA T^TAA
AAT^T		 5' - AT
MslI CAYNNNNRTG CAYNN^NNRTG
GTYNN^NNRAC		 Blunt
MspI HpaII CCGG C^CGG
GGC^C		 5' - GC
Msp17I AcyI GRCGYC GR^CGYC
CRGC^YG		 5' - GC
Msp20I BalI TGGCCA TGG^CCA
ACC^GGT		 Blunt
Msp67I ScrFI CCNGG CC^NGG
GGN^CC		 5' - N
MspA1I NspBII CMGCKG CMG^CKG
GMC^GKC		 Blunt
MspB4I HgiCI GGYRCC G^GYRCC
CCYRG^G		 5' - CYRG
MspCI AflII CTTAAG C^TTAAG
GAATT^C		 5' - AATT
MspR9I ScrFI CCNGG CC^NGG
GGN^CC		 5' - N
MspSWI SwaI ATTTAAAT ATTT^AAAT
TAAA^TTTA		 Blunt
MspV281I HgiAI GWGCWC GWGCW^C
C^WCGWG		 WGCW - 3'
MspYI BsaAI YACGTR YAC^GTR
YTG^CAR		 Blunt
MssI PmeI GTTTAAAC GTTT^AAAC
CAAA^TTTG		 Blunt
MstI TGCGCA TGC^GCA
ACG^CGT		 Blunt
MstII SauI CCTNAGG CC^TNAGG
GGANT^CC		 5' - ANT
MthZI MaeI CTAG C^TAG
GAT^C		 5' - AT
MunI MfeI CAATTG C^AATTG
GTTAA^C		 5' - TTAA
MvaI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
Mva1269I BsmI GAATGC GAATGCN^
CTTAC^GN		 CN - 3'
MvnI FnuDII CGCG CG^CG
GC^GC		 Blunt
MvrI PvuI CGATCG CGAT^CG
GC^TAGC		 AT - 3'
MwoI GCNNNNNNNGC GCNNNNN^NNGC
CGNN^NNNNNCG		 NNN - 3'
MxaI SacI GAGCTC GAG^CTC
CTC^GAG		 Blunt
MalI MboI GATC GA^TC
CT^AG		 Blunt
MauBI CGCGCGCG CG^CGCGCG
GCGCGC^GC		 5' - GCGC
MluCI TspEI AATT ^AATT
TTAA^		 5' - TTAA
MreI Sse232I CGCCGGCG CG^CCGGCG
GCGGCC^GC		 5' - GGCC