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PCR Cloning

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GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme Prototype Sequence Cut Site Overhang
AaaI XmaIII CGGCCG C^GGCCG
GCCGG^C		 5' - CCGG
AagI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
AarI CACCTGC CACCTGCNNNN^NNNN
GTGGACGNNNNNNNN^		 5' - NNNN
AasI DrdI GACNNNNNNGTC GACNNNN^NNGTC
CTGNN^NNNNCAG		 NN - 3'
AatI StuI AGGCCT AGG^CCT
TCC^GGA		 Blunt
AatII GACGTC GACGT^C
C^TGCAG		 ACGT - 3'
AauI Bsp1407I TGTACA T^GTACA
ACATG^T		 5' - CATG
AbaI BclI TGATCA T^GATCA
ACTAG^T		 5' - CTAG
AbeI BbvCI CCTCAGC CC^TCAGC
GGAGT^CG		 5' - AGT
AbrI XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
AccI GTMKAC GT^MKAC
CAMK^TG		 5' - MK
AccII FnuDII CGCG CG^CG
GC^GC		 Blunt
AccIII BspMII TCCGGA T^CCGGA
AGGCC^T		 5' - GGCC
Acc16I MstI TGCGCA TGC^GCA
ACG^CGT		 Blunt
Acc36I BspMI ACCTGC ACCTGCNNNN^NNNN
TGGACGNNNNNNNN^		 5' - NNNN
Acc65I KpnI GGTACC G^GTACC
CCATG^G		 5' - CATG
Acc113I ScaI AGTACT AGT^ACT
TCA^TGA		 Blunt
AccB1I HgiCI GGYRCC G^GYRCC
CCYRG^G		 5' - CYRG
AccB2I HaeII RGCGCY RGCGC^Y
R^CGCGY		 GCGC - 3'
AccB7I PflMI CCANNNNNTGG CCANNNN^NTGG
GGTN^NNNNACC		 NNN - 3'
AccBSI BsrBI CCGCTC CCG^CTC
GGC^GAG		 Blunt
AccEBI BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
AceI TseI GCWGC G^CWGC
CGWC^G		 5' - GWC
AceII NheI GCTAGC GCTAG^C
C^GATCG		 CTAG - 3'
AceIII CAGCTC CAGCTCNNNNNNN^NNNN
GTCGAGNNNNNNNNNNN^		 5' - NNNN
AciI CCGC C^CGC
GGC^G		 5' - GC
AclI AACGTT AA^CGTT
TTGC^AA		 5' - GC
AclNI SpeI ACTAGT A^CTAGT
TGATC^A		 5' - GATC
AclWI BinI GGATC GGATCNNNN^N
CCTAGNNNNN^		 5' - N
AcpI AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
AcpII PflMI CCANNNNNTGG CCANNNN^NTGG
GGTN^NNNNACC		 NNN - 3'
AcrII BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
AcsI ApoI RAATTY R^AATTY
RTTAA^Y		 5' - TTAA
AcuI Eco57I CTGAAG CTGAAGNNNNNNNNNNNNNNNN^
GACTTCNNNNNNNNNNNNNN^NN		 NN - 3'
AcvI PmaCI CACGTG CAC^GTG
GTG^CAC		 Blunt
AcyI GRCGYC GR^CGYC
CRGC^YG		 5' - GC
AdeI DraIII CACNNNGTG CACNNN^GTG
GTG^NNNCAC		 NNN - 3'
AeuI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
AfaI RsaI GTAC GT^AC
CA^TG		 Blunt
Afa22MI PvuI CGATCG CGAT^CG
GC^TAGC		 AT - 3'
Afa16RI PvuI CGATCG CGAT^CG
GC^TAGC		 AT - 3'
AfeI Eco47III AGCGCT AGC^GCT
TCG^CGA		 Blunt
AflI AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
AflII CTTAAG C^TTAAG
GAATT^C		 5' - AATT
AflIII ACRYGT A^CRYGT
TGRYC^A		 5' - GRYC
AgeI ACCGGT A^CCGGT
TGGCC^A		 5' - GGCC
AglI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
AhaI CauII CCSGG CC^SGG
GGS^CC		 5' - S
AhaII AcyI GRCGYC GR^CGYC
CRGC^YG		 5' - GC
AhaIII TTTAAA TTT^AAA
AAA^TTT		 Blunt
AhaB8I KpnI GGTACC G^GTACC
CCATG^G		 5' - CATG
AhdI Eam1105I GACNNNNNGTC GACNNN^NNGTC
CTGNN^NNNCAG		 N - 3'
AhlI SpeI ACTAGT A^CTAGT
TGATC^A		 5' - GATC
AhyI SmaI CCCGGG C^CCGGG
GGGCC^C		 5' - GGCC
AitI Eco47III AGCGCT AGC^GCT
TCG^CGA		 Blunt
AjnI EcoRII CCWGG ^CCWGG
GGWCC^		 5' - GGWCC
AjoI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
AleI OliI CACNNNNGTG CACNN^NNGTG
GTGNN^NNCAC		 Blunt
AlfI GCANNNNNNTGC GCANNNNNNTGCNNNNNNNNNNNN^
CGTNNNNNNACGNNNNNNNNNN^NN		 NN - 3'
AliI BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
AliAJI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
AloI GAACNNNNNNTCC GAACNNNNNNTCCNNNNNNNNNNNN^
CTTGNNNNNNAGGNNNNNNN^NNNNN		 NNNNN - 3'
AluI AGCT AG^CT
TC^GA		 Blunt
AlwI BinI GGATC GGATCNNNN^N
CCTAGNNNNN^		 5' - N
Alw21I HgiAI GWGCWC GWGCW^C
C^WCGWG		 WGCW - 3'
Alw26I BsmAI GTCTC GTCTCN^NNNN
CAGAGNNNNN^		 5' - NNNN
Alw44I ApaLI GTGCAC G^TGCAC
CACGT^G		 5' - ACGT
AlwNI CAGNNNCTG CAGNNN^CTG
GTC^NNNGAC		 NNN - 3'
AlwXI BbvI GCAGC GCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^		 5' - NNNN
Ama87I AvaI CYCGRG C^YCGRG
GYGCR^C		 5' - YGCR
AocI SauI CCTNAGG CC^TNAGG
GGANT^CC		 5' - ANT
AocII SduI GDGCHC GDGCH^C
C^DCGHG		 DGCH - 3'
AorI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
Aor13HI BspMII TCCGGA T^CCGGA
AGGCC^T		 5' - GGCC
Aor51HI Eco47III AGCGCT AGC^GCT
TCG^CGA		 Blunt
AosI MstI TGCGCA TGC^GCA
ACG^CGT		 Blunt
AosII AcyI GRCGYC GR^CGYC
CRGC^YG		 5' - GC
ApaI GGGCCC GGGCC^C
C^CCGGG		 GGCC - 3'
ApaBI GCANNNNNTGC GCANNNNN^TGC
CGT^NNNNNACG		 NNNNN - 3'
ApaCI BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
ApaLI GTGCAC G^TGCAC
CACGT^G		 5' - ACGT
ApaORI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
ApeKI TseI GCWGC G^CWGC
CGWC^G		 5' - GWC
ApiI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
ApoI RAATTY R^AATTY
RTTAA^Y		 5' - TTAA
ApyI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
AquI AvaI CYCGRG C^YCGRG
GYGCR^C		 5' - YGCR
AscI GGCGCGCC GG^CGCGCC
CCGCGC^GG		 5' - GCGC
AseI VspI ATTAAT AT^TAAT
TAAT^TA		 5' - AT
AseII CauII CCSGG CC^SGG
GGS^CC		 5' - S
AsiI BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
AsiAI AgeI ACCGGT A^CCGGT
TGGCC^A		 5' - GGCC
AsiSI SgfI GCGATCGC GCGAT^CGC
CGC^TAGCG		 AT - 3'
AsnI VspI ATTAAT AT^TAAT
TAAT^TA		 5' - AT
AspI Tth111I GACNNNGTC GACN^NNGTC
CTGNN^NCAG		 5' - N
Asp700I XmnI GAANNNNTTC GAANN^NNTTC
CTTNN^NNAAG		 Blunt
Asp713I PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
Asp718I KpnI GGTACC G^GTACC
CCATG^G		 5' - CATG
Asp745I AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
AspAI BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
AspA2I AvrII CCTAGG C^CTAGG
GGATC^C		 5' - GATC
AspEI Eam1105I GACNNNNNGTC GACNNN^NNGTC
CTGNN^NNNCAG		 N - 3'
AspHI HgiAI GWGCWC GWGCW^C
C^WCGWG		 WGCW - 3'
Asp10HI AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
Asp10HII PflMI CCANNNNNTGG CCANNNN^NTGG
GGTN^NNNNACC		 NNN - 3'
Asp26HI BsmI GAATGC GAATGCN^
CTTAC^GN		 CN - 3'
Asp27HI BsmI GAATGC GAATGCN^
CTTAC^GN		 CN - 3'
Asp35HI BsmI GAATGC GAATGCN^
CTTAC^GN		 CN - 3'
Asp36HI BsmI GAATGC GAATGCN^
CTTAC^GN		 CN - 3'
Asp40HI BsmI GAATGC GAATGCN^
CTTAC^GN		 CN - 3'
Asp50HI BsmI GAATGC GAATGCN^
CTTAC^GN		 CN - 3'
AspLEI HhaI GCGC GCG^C
C^GCG		 CG - 3'
AspMI StuI AGGCCT AGG^CCT
TCC^GGA		 Blunt
AspMDI MboI GATC ^GATC
CTAG^		 5' - CTAG
AspNI NlaIV GGNNCC GGN^NCC
CCN^NGG		 Blunt
AspS9I AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
AssI ScaI AGTACT AGT^ACT
TCA^TGA		 Blunt
AstWI AcyI GRCGYC GR^CGYC
CRGC^YG		 5' - GC
AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
AsuIII AcyI GRCGYC GR^CGYC
CRGC^YG		 5' - GC
AsuC2I CauII CCSGG CC^SGG
GGS^CC		 5' - S
AsuHPI HphI GGTGA GGTGANNNNNNNN^
CCACTNNNNNNN^N		 N - 3'
AsuNHI NheI GCTAGC G^CTAGC
CGATC^G		 5' - GATC
AtsI Tth111I GACNNNGTC GACN^NNGTC
CTGNN^NCAG		 5' - N
AvaI CYCGRG C^YCGRG
GYGCR^C		 5' - YGCR
AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
AvcI AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
AviII MstI TGCGCA TGC^GCA
ACG^CGT		 Blunt
AvrII CCTAGG C^CTAGG
GGATC^C		 5' - GATC
AvrBII AvrII CCTAGG C^CTAGG
GGATC^C		 5' - GATC
AxyI SauI CCTNAGG CC^TNAGG
GGANT^CC		 5' - ANT
AanI PsiI TTATAA TTA^TAA
AAT^ATT		 Blunt
AbsI CCTCGAGG CC^TCGAGG
GGAGCT^CC		 5' - AGCT
AcoI CfrI YGGCCR Y^GGCCR
YCCGG^R		 5' - CCGG
AfiI GCNNNNNNNGG GCNNNNN^NNGG
CGNN^NNNNNCC		 NNN - 3'
AjiI BtrI CACGTC CAC^GTC
GTG^CAG		 Blunt
AjuI ^
^		 Blunt
AluBI AluI AGCT AG^CT
TC^GA		 Blunt
AsiGI AgeI ACCGGT A^CCGGT
TGGCC^A		 5' - GGCC