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PCR Cloning

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GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme Prototype Sequence Cut Site Overhang
EacI BinI GGATC GGATCNNNN^N
CCTAGNNNNN^		 5' - N
EaeI CfrI YGGCCR Y^GGCCR
YCCGG^R		 5' - CCGG
Eae46I SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
EaeAI SmaI CCCGGG C^CCGGG
GGGCC^C		 5' - GGCC
EagI XmaIII CGGCCG C^GGCCG
GCCGG^C		 5' - CCGG
EagBI PvuI CGATCG CGAT^CG
GC^TAGC		 AT - 3'
EagMI AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
Eam1104I Ksp632I CTCTTC CTCTTCN^NNN
GAGAAGNNNN^		 5' - NNN
Eam1105I GACNNNNNGTC GACNNN^NNGTC
CTGNN^NNNCAG		 N - 3'
EarI Ksp632I CTCTTC CTCTTCN^NNN
GAGAAGNNNN^		 5' - NNN
EcaI BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
EciI GGCGGA GGCGGANNNNNNNNNNN^
CCGCCTNNNNNNNNN^NN		 NN - 3'
Eci125I BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
EclI PvuII CAGCTG CAG^CTG
GTC^GAC		 Blunt
Ecl136II SacI GAGCTC GAG^CTC
CTC^GAG		 Blunt
EclHKI Eam1105I GACNNNNNGTC GACNNN^NNGTC
CTGNN^NNNCAG		 N - 3'
EclRI SmaI CCCGGG C^CCGGG
GGGCC^C		 5' - GGCC
EclXI XmaIII CGGCCG C^GGCCG
GCCGG^C		 5' - CCGG
Ecl18kI ScrFI CCNGG ^CCNGG
GGNCC^		 5' - GGNCC
Ecl37kI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
Ecl2zI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
Eco24I HgiJII GRGCYC GRGCY^C
C^RCGYG		 RGCY - 3'
Eco31I GGTCTC GGTCTCN^NNNN
CCAGAGNNNNN^		 5' - NNNN
Eco32I EcoRV GATATC GAT^ATC
CTA^TAG		 Blunt
Eco47I AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
Eco47III AGCGCT AGC^GCT
TCG^CGA		 Blunt
Eco52I XmaIII CGGCCG C^GGCCG
GCCGG^C		 5' - CCGG
Eco56I NaeI GCCGGC G^CCGGC
CGGCC^G		 5' - GGCC
Eco57I CTGAAG CTGAAGNNNNNNNNNNNNNNNN^
GACTTCNNNNNNNNNNNNNN^NN		 NN - 3'
Eco64I HgiCI GGYRCC G^GYRCC
CCYRG^G		 5' - CYRG
Eco72I PmaCI CACGTG CAC^GTG
GTG^CAC		 Blunt
Eco78I NarI GGCGCC GGC^GCC
CCG^CGG		 Blunt
Eco81I SauI CCTNAGG CC^TNAGG
GGANT^CC		 5' - ANT
Eco88I AvaI CYCGRG C^YCGRG
GYGCR^C		 5' - YGCR
Eco91I BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
Eco105I SnaBI TACGTA TAC^GTA
ATG^CAT		 Blunt
Eco130I StyI CCWWGG C^CWWGG
GGWWC^C		 5' - GWWC
Eco147I StuI AGGCCT AGG^CCT
TCC^GGA		 Blunt
Eco255I ScaI AGTACT AGT^ACT
TCA^TGA		 Blunt
Eco1831I CauII CCSGG ^CCSGG
GGSCC^		 5' - GGSCC
EcoA4I Eco31I GGTCTC GGTCTCN^NNNN
CCAGAGNNNNN^		 5' - NNNN
EcoHI CauII CCSGG ^CCSGG
GGSCC^		 5' - GGSCC
EcoHK31I CfrI YGGCCR Y^GGCCR
YCCGG^R		 5' - CCGG
EcoICRI SacI GAGCTC GAG^CTC
CTC^GAG		 Blunt
Eco75KI HgiJII GRGCYC GRGCY^C
C^RCGYG		 RGCY - 3'
Eco57MI CTGRAG CTGRAGNNNNNNNNNNNNNNNN^
GACRTCNNNNNNNNNNNNNN^NN		 NN - 3'
EcoNI CCTNNNNNAGG CCTNN^NNNAGG
GGANNN^NNTCC		 5' - N
EcoO44I Eco31I GGTCTC GGTCTCN^NNNN
CCAGAGNNNNN^		 5' - NNNN
EcoO65I BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
EcoO109I DraII RGGNCCY RG^GNCCY
RCCNG^GY		 5' - CNG
EcoO128I BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
EcoRI GAATTC G^AATTC
CTTAA^G		 5' - TTAA
EcoRII CCWGG ^CCWGG
GGWCC^		 5' - GGWCC
EcoRV GATATC GAT^ATC
CTA^TAG		 Blunt
EcoT14I StyI CCWWGG C^CWWGG
GGWWC^C		 5' - GWWC
EcoT22I AvaIII ATGCAT ATGCA^T
T^ACGTA		 TGCA - 3'
EcoT38I HgiJII GRGCYC GRGCY^C
C^RCGYG		 RGCY - 3'
EcoVIII HindIII AAGCTT A^AGCTT
TTCGA^A		 5' - TCGA
Eco13kI ScrFI CCNGG ^CCNGG
GGNCC^		 5' - GGNCC
Eco21kI ScrFI CCNGG ^CCNGG
GGNCC^		 5' - GGNCC
Eco27kI AvaI CYCGRG C^YCGRG
GYGCR^C		 5' - YGCR
Eco29kI SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
Eco53kI SacI GAGCTC GAG^CTC
CTC^GAG		 Blunt
Eco137kI ScrFI CCNGG ^CCNGG
GGNCC^		 5' - GGNCC
EgeI NarI GGCGCC GGC^GCC
CCG^CGG		 Blunt
EheI NarI GGCGCC GGC^GCC
CCG^CGG		 Blunt
ErhI StyI CCWWGG C^CWWGG
GGWWC^C		 5' - GWWC
ErhB9I PvuI CGATCG CGAT^CG
GC^TAGC		 AT - 3'
ErhB9II StyI CCWWGG C^CWWGG
GGWWC^C		 5' - GWWC
ErpI AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
EsaBC3I TaqI TCGA TC^GA
AG^CT		 Blunt
EsaBC4I HaeIII GGCC GG^CC
CC^GG		 Blunt
EspI GCTNAGC GC^TNAGC
CGANT^CG		 5' - ANT
Esp3I CGTCTC CGTCTCN^NNNN
GCAGAGNNNNN^		 5' - NNNN
Esp4I AflII CTTAAG C^TTAAG
GAATT^C		 5' - AATT
Esp1396I PflMI CCANNNNNTGG CCANNNN^NTGG
GGTN^NNNNACC		 NNN - 3'
EcoP15I CAGCAG CAGCAGNNNNNNNNNNNNNNNNNNNNNNNNN^NN
GTCGTCNNNNNNNNNNNNNNNNNNNNNNNNNNN^		 5' - NN