GenCRISPR SpCas9 demonstrated high gene editing efficiency in both in vitro cleavage tests and HEK293T cells.
In vitro cleavage test

Gene knockout efficiency_HEK293 T cell

Cas9 Nucleases
Recombinant wild-type S. pyogenes Cas9 nucleases, purified from E. coli expression and contains various nuclear localization sequences (NLSs).
Cat. No. | Product Name | Quantity | Price | Order |
---|---|---|---|---|
RC00001
|
RC00001 |
|
$150.00 0.1 mg |
|
RC00002
|
GenCRISPR™ Ultra eSpCas9-2NLS-Research |
|
$10.00 1 mg |
|
Z03385
|
GenCrispr Cas9-C-NLS Nuclease |
|
$146.00 |
|
Z03386
|
GenCrispr Cas9 Nuclease |
|
$35.00 |
|
Z03388
|
GenCrispr Cas9-N-NLS Nuclease |
|
$146.00 |
|
Z03389
|
GenCrispr NLS-Cas9-NLS Nuclease |
|
$146.00 |
|
Z03390
|
GenCrispr NLS-Cas9-D10A Nickase |
|
$99.00 |
|
Z03393
|
GenCrispr NLS-Cas9-EGFP Nuclease |
|
$188.00 |
|
Z03467
|
GenCrispr NLS-Cas9-EGFP Nuclease |
|
$241.00 |
|
Z03468
|
GenCrispr Cas9-N-NLS Nuclease |
|
$188.00 |
|
Z03469
|
GenCrispr NLS-Cas9-NLS Nuclease |
|
$188.00 |
|
Z03470
|
GenCrispr eSpCas9-N-NLS Nuclease |
|
$240.00 |
|
Z03621
|
GenCRISPR™ Ultra NLS-Cas9-Research |
|
$695.00 |
|
Z03622
|
GenCRISPR™ Ultra eSpCas9-2NLS-Research |
|
$765.00 |
|
Z03623-GMP
|
GenCRISPR™ Ultra NLS-Cas9-GMP |
|
$6900.00 2.5 mg |
|
Z03699
|
GenCRISPR™ SaCas9 2NLS Nuclease |
|
$210.00 |
|
Z03702
|
GenCRISPR™ Cas9 v1.2 |
|
- 1 mg |
|
Cas12a Nuclease
Recombinant Acidaminococcus sp. (strain BV3L6) Cas12a (cpf1) nuclease or Lachnospiraceae bacterium ND2006 Cas12a nuclease, purified from E. coli expression, and contains nuclear localization sequences (NLSs).
Cat. No. | Product Name | Quantity | Price | Order |
---|---|---|---|---|
Z03502
|
GenCRISPR™ Cas12a (Cpf1) Nuclease |
|
$105.00 |
|
Cas13a Nuclease
Recombinant Leptotrichia wadei Cas13a nuclease or Leptotrichia buccalis Cas13a nuclease, purified from E. coli expression.
Cat. No. | Product Name | Quantity | Price | Order |
---|---|---|---|---|
Z03486
|
GenCRISPR™ Cas13a (C2c2) Nuclease |
|
$189.00 |
|
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Base Editor
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Gene Editing Kits
Cat. No. | Product Name | Quantity | Price | Order |
---|---|---|---|---|
L00688
|
GenCrispr Mutation Detection Kit |
|
$180.00 25 reactions |
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L00689
|
GenCrispr sgRNA Screening Kit |
|
$166.00 30 reactions |
|
L00694
|
GenCrispr sgRNA Synthesis Kit |
|
$379.00 20 reactions |
|
Z03396
|
GenCrispr T7 Endonuclease I |
|
$55.00 |
|
Cas Nuclease Antibodies
Cat. No. | Product Name | Quantity | Price | Order |
---|---|---|---|---|
A01885
|
GenCrispr Cas9 Antibody, pAb, Rabbit |
|
$79.00 |
|
A01935
|
GenCRISPR™ SpCas9 Antibody (4A1), mAb, Mouse |
|
$99.00 40 μg |
|
A01936
|
GenCRISPR™ SpCas9 Antibody (14B6), mAb, Mouse |
|
$99.00 40 μg |
|
A01951
|
GenCRISPR™ SaCas9 Antibody (11C12), mAb, Mouse |
|
$99.00 40 μg |
|
A01952
|
GenCRISPR™ SaCas9 Antibody (26H10), mAb, Mouse |
|
$99.00 40 μg |
|
A01957
|
GenCRISPR™ FnCpf1 Antibody (9H6), mAb, Mouse |
|
$99.00 40 μg |
|
A01958
|
GenCRISPR™ FnCpf1 Antibody (14H3), mAb, Mouse |
|
$99.00 40 μg |
|
GenCRISPR SpCas9 demonstrated high gene editing efficiency in both in vitro cleavage tests and HEK293T cells.
In vitro cleavage test
Gene knockout efficiency_HEK293 T cell
Gene knockout efficiency_293 T cells & Jurkat cells
GenCRISPR SpCas9 is used for gene knockout in 293T cells and Jurkat cells. As shown in the figure, the gene knockout efficiency of three batches shows no significant difference.
GenCRISPR SpCas9 is used for gene knockout in 293T cells and Jurkat cells. As shown in the figure, the gene knockout efficiency is equivalent to that of the leading supplier I.
The fidelity and editing efficiency of GenCRISPR eSpCas9 Protein were evaluated against wildtype Cas9 on Jurkat cells and primary T cells. GenCRISPR eSpCas9 exhibited a superior ability to reduce off-target edits.
Low off-target effects_Jurkat cells
Low off-target effects_ Primary T cells
GenCRISPR eSpCas9 exhibits comparable knockout and knock-in efficiency to the wildtype SpCas9 in both Jurkat cells and primary T cells.
High Knockout_ Jurkat cells and Primary T cell
High Knock-in_ Primary T cell
The GenCRIPSR™ Ultra eSpCas9 (mt) shows high batch-to-batch consistency, with no significant differences in cell viability and consistent knockout/knock-in efficiency across the three batches at any concentration.
KO at TRAC sites in HEK293T cells
GFP KI at RAB11A in Jurkat cells
GenCRISPR eSpCas9 exhibits lower off-target effects compared to HiFi Cas9 products from leading suppliers, while its knockout and knock-in efficiencies are equivalent to or exceed those of the leading suppliers.
Low off-target effects_HEK293 T cell
High Knockout efficiency_HEK293 T cell
High Knock-in efficiency_Primary T cell
Gene editing efficiency in Jurkat cells using ABE8e RNP
High A to G conversion across different doses
ABE8e protein effectively knocks out B2M protein
Gene editing efficiency in HEK293T cells using PE7 RNP at 50 pmol
PE7 mediated targeted 5 bp deletions at the HEK3 locus in HEK293 cells
No significant impact on cell viability
Enabling RNP-mediated CRISPR gene editing and transforming life science research
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