Company News » GenCRISPR™ Cas13a (C2c2) Nuclease

GenCRISPR™ Cas13a (C2c2) Nuclease

GenCRISPR Cas13a (C2c2) Nuclease is an RNA-guided, RNA-targeting CRISPR enzyme, commonly referred to as C2c2. This enzyme is a member of Type VI CRISPR family containing a single protein effector with two HEPN domains. The Cas13a exhibits two RNase activities. The first activity, a cis- sequence-specific RNA guided cleavage, activates the secondary RNase activity of the enzyme, a non-specific trans- ribonuclease activity. This Cas13a protein can be applied to detect single RNA molecules with proven high specificity.
Z03486
$189.00

Ask us a question
Description

GenCRISPR Cas13a (C2c2) Nuclease is an RNA-guided, RNA-targeting CRISPR enzyme, commonly referred to as C2c2. This enzyme is a member of Type VI CRISPR family containing a single protein effector with two HEPN domains.  The Cas13a exhibits two RNase activities. The first activity, a cis- sequence-specific RNA guided cleavage, activates the secondary RNase activity of the enzyme, a non-specific trans- ribonuclease activity. This Cas13a protein can be applied to detect single RNA molecules with proven high specificity.

Recently, Cas13a has been leveraged as a diagnostic nucleic acid detection tool. Using a combination of the Cas13a cis- and trans- RNase activity and a non-target RNA tagged with a cleavage reporter ((fluorescence), the Cas13 system provides a route for reporting of the presence of a specific single RNA sequence in a sample with high specificity.
The GenScript Cas13a contains a His-tag at the C-terminal.
Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Applications
crRNA-dependent RNA cleavage

Properties
Expression System Recombinant Cas13a expressed by E.coli
Species Leptotrichia wadei
Tag C-terminal His Tag
Apparent Molecular Weight ~140 kDa, on SDS-PAGE under non-reducing conditions
Concentration Please refer to the COA for the specific lot.
Formulation Supplied as a solution of 10 mM Tris, pH 8.0, 300 mM NaCl, 1.0 mM DTT, and 50% glycerol. 
Storage & Stability This product remains stable up to 12 months at -20℃. Avoid repeated freeze-thaw cycles.
Accession# U2PSH1
Quality Control Specifications
AssaySpecifications
AppearanceClear, colorless liquid
Purity by SDS-PAGE≥ 95%
Residual DNaseUndetectable
Residual RNaseUndetectable
Endotoxin Level< 0.2 EU/μg of the protein by gel clotting method