HELZ cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	HELZ
Entrez Gene ID	100913867
Full Name	helicase with zinc finger
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003768578.3	XP_003768626.1	probable helicase with zinc finger domain
XM_003768578.2	XP_003768626.1	probable helicase with zinc finger domain
XM_031968638.1	XP_031824498.1	probable helicase with zinc finger domain

Pathways from BioSystems
Gene Ontology

The following HELZ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HELZ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa235367	XM_031968638.1 Latest version!	Sarcophilus harrisii helicase with zinc finger (HELZ), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa130608	XM_003768578.3 Latest version!	Sarcophilus harrisii helicase with zinc finger (HELZ), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OSa130608	XM_003768578.2	Sarcophilus harrisii helicase with zinc finger (HELZ), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HELZ ( XM_031968638.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031824498.1 Sarcophilus harrisii helicase with zinc finger (HELZ), mRNA.

Price & Availability↑

CloneID	OSa235367
Clone ID Related Accession (Same CDS sequence)	XM_031968638.1
Accession Version	XM_031968638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	probable helicase with zinc finger domain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGACTACT CTACCCTGGT AGCTCCTCTT TGTGCTACTC TTAATGCCCG TTCGTCAACG 
GCACAACTAA ACCACACTTA CCTGTCTTCG GGCACCAACC CGGATCCCAT AGAGACAGAC 
CACCAGGAGC TTAGTTGGGC TTCACACCAA AGTTTCTGCA GACTTGGTTT TCCCTCCACC 
ACTCTTCGCT GTTTTGTGAG GGTATATTTC AGAAATCGCT GGGTAAAGAC TGTCCACCCA 
GTTGTACATC AATACTGTTT GATTTCAAGC ACACATTCCA CCTTTCAGAT GCCACAGAAA 
GAAGACATTC TTAAACAACG AGTTGTAGTT GTTACATTGA ATACTTCACA GTACCTTTGT 
CAACTGGATC TTGAACCTGG GTTTTTTACA CACATTCTTT TGGATGAAGC TGCACAGGCC 
ATGGAGTGTG AAACCATTAT GCCTCTGGCA TTAGCTAATA AAAACACACG AATTGTTTTG 
GCTGGAGACC ATATGCAACT CAGTCCTTTT GTCTACAGTG AGTTTGCCAG GGAGAGAAAC 
CTTCATGTTT CATTACTGGA TCGGCTCTAT GAACATTATC CTGCAGAGTT TCCATGTCGG 
ATCCTACTGT GTGAGAACTA TCGTTCCCAT GAAGCTATCA TCAATTACAC ATCAGAACTT 
TTCTATGAGG GGAAGTTGAT GGCAAGTGGA AAACAACCAG CACACAAAGA TTTTTATCCA 
TTAACCTTCT TTACAGCACG AGGAGAAGAT GTACAGGAAA AAAATAGTAC AGCCTTTTAT 
AATAATGCAG AGGTATTTGA AGTGGTGGAA CGGGTGGAAG AGTTAAGGAG AAAGTGGCCT 
GTAGCTTGGG GAAAATTAGA TGATGGAAGT ATAGGTGTAG TCACTCCTTA TGCTGATCAG 
GTGTTCAGGA TCCGGGCAGA ACTTCGAAAA AAGAGATTAT CTGATGTCAA TGTGGAAAGA 
GTGCTCAATG TGCAGGGAAA GCAGTTTAGG GTTTTGTTTC TGAGTACAGT ACGTACCCGG 
CACACTTGCA AACATAAACA AACCCCAATT AAAAGGAAAG AGCAACTGTT AGAAGATTCA 
ACAGAGGATT TGGATTATGG TTTTTTGTCC AACTACAAAC TTCTCAACAC CGCCATAACA 
AGAGCACAGT CCTTGGTAGC TGTGGTGGGT GATCCTATTG CGCTGTGCTC TATTGGAAGA 
TGCAGGAAAT TTTGGGAGCG GTTTATTGCC TTGTGTCATG ACAACAAAAG CCTACATGGT 
ATCACTTTTG AACAGATCAA AACACAGTTA GAGGCTTTGG AGCTAAAGAA AACATATGTG 
TTGAATCCAC TTGCTCCTGA ATTTATCCCT CGGGCTCTAA GACAGCAACA TTTGGGAAAT 
ACCAGCAAAC AGCAGCAATC ACCACCCAAG GGAAAAGTCC ACCATCATAA CCAAAATGAA 
CACTTCCAGA ATGAAGGAAT TGTTCAACCC AATCCTTCAG TACTCATTGG CAATCCTATT 
AGAGCATATA CACCTCCTTC CCCTCTTGGA CCTCACCCAA ATATGGGAAA GTCTCCTAGT 
CCTGTTCAAA GGATAGATCC GCACACTGGG ACTAGTATTC TTTATGTACC TGCTGTCTAT 
GGAGGAAATG TGGTTATGTC CGTGCCTTTA CCTGTACCAT GGACAGGGTA CCAGGGTAGG 
TTTGCAGTTG ACCCTCGAAT CATTACACAT CAGGCAGCGA TGGCATATAA TATGAACCTG 
TTGCAGACAC ATGGACGGGG GTCTCCTATA CCATATGGCC TTGGACATCA TTCACCAGTT 
AGTATAGGGC AACAGCAAAT TCAGCATCAG GAAAAGGATC AACATGAACA AAATCGAAAT 
GGTAAAAGTG ATACTAATCC TGGACCTGAA ATAAATAAAA TTCGTACTCC AGAGAAAAAG 
CCTCTAGAAT CAAAACAGAA TGATTTAGAT TCAAATCCAC AAAACCGAAG TCCAGAAACT 
CGCCCTGGTG TTGCTTATCC CAATCCCAAA TTTCATCGCA AAGATAATCT CAACCCAAGA 
CACCTAAACC TTCATCTTAC GGCTCCCCAC ACACAATATG CAGTCCCTCA TCGACATTAT 
CAGCACTTAT CCCAGCTACC AAGACAACCA TTTCCAGTTC AACAGCCCTC AAGTTTAAAT 
CAGCAGCAAA ATCATTTCCC TGAGCAACTG ACTCAAATAA CACCTCAACC AAACCAAGCA 
GTCCAGCAGC ATAACCACTT GAATCAACAA ACTCAGCAGC AACCCTCTCA ACTTTCTCCT 
ACATATCAAA TAGGTCCCAG TCATTCCTTC TTCAATAATG CAGTCTCTCA TCGACCACAT 
TCTCCTGCTG TAGATGCAGT AGTATCTGAA CAGCACCCCC CTTCGATGCT GCAAGAAGCT 
AACTCTCTGA GATCCATTGC ACAACACAAC ACGATGATAC CCCCCACTCA CTTGAATAAT 
TTTATTGAAG AAAGTCCTCC AGGATTACCA TTAGGAGAAG CTTTAGAACG TATGCATGGG 
AATGTGGCCT TAGAAACATT AAGGCAACAA CAAGCAAGAA TACAGCAGTG GAGTGAGCAT 
AATGCCTATC TCAGTCAAAG CAGTCTTCCA TATCCACACT ATCACCATCC TCACCTGCAG 
CATCTTCCCC AGCAACCTGC AGGATTACAT CAACAGCCAG TTAGAGCAAA CTGGAAACTT 
GCCAGTAATG CAGAAGATGA AGCAGAAGCA ACACATTCAA GGTTTCAAGA CTTACTCAGA 
GAGCTGGCTC ATCATGATCA AAGTGAAACA CGGGAGCTAA CTGAAATGCC ACCACCTCAG 
TCAAGACTTT TGCAATATAG ACAAATTCAA ACTAGAAGCC CACCAGCAAA CCCTTCTTCT 
TCATCTAATT CAAATCATAG TGGCCAATTT TCTAACTTCA ACGACAACAT TAGAGACATT 
GAAATGGCCA GCAATCCAGC ATTTCAGCAG CATCTGCCAC CCCAAATATA TAATCCCTCA 
TTTTCAATAC CATCTGAAAA CCTTACCCCT CCTCCTTTGA AATACCTGCC ACCAGATGGA 
TCCTGGACCT TTGCTAATTT ACATCAGAAT CACTTAATGG GACAGGGCTT TCACTATGGC 
CTACCACCAT TACCCCACAG ACCACAACAG AACTCTTTTA TACAAATTCA GAATCAACAT 
GTGGTTGGTC AGGAGCCATT TCACCCACTG TCATCTCGTG TGGTATCTGC TTCTTCGCTC 
CACAGCTTAG AAGAGTATGA ACCACGAGGA CCAGGCAGGC CATTGTACCA AAGAAGAATT 
TCATCTAGTT CAGTACCGCC TTGTTCTGAA GAGTTAAACA CTCCTCAAGA TAGTCATGCT 
CAGGGTAAAG AGCATCAGGA CCATAATAAT CAATCTTCTT TCAACTATTC ATCACCGGAG 
TTGTGGGTAA ATACCACCTC ATCTGCTCCA CATCAGAACA TTCCATGCAA TGGATCCAGC 
AGAACAGCCC CGCCCAGAGA ATTGTTAGCC CCATCAAAGC CCTTGAAATC CTCTGAGGAT 
AACCTGAAAT CTGAAAACCT TCAGCTGTCC AATTCCTTCA ACTACAGCAT GTTGCAGCAT 
CTAGGCCAGT TCCCACCCCT AATGCCCAAC AAGCAGATTG TGGAATCAAA CGGCAACAGC 
CAGCCAAGTT CTGGGGGGAA CAAGCCTATT ATGTCCTACG CAAGTGCTCT GCGGGCACCT 
CCAAAGCCCA AACCTCCACC TGAACAGACA AAAAAGAAGA GTGACCCTTT GTCTCTGTTT 
CAGGAACTTA GTTTAGGTAG CTCATCAGGT AGCAATGGCT TTTATTCCTA TTTTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031824498.1
CDS	1..3840
Translation	MDYSTLVAPLCATLNARSSTAQLNHTYLSSGTNPDPIETDHQELSWASHQSFCRLGFPSTTLRCFVRVYFRNRWVKTVHPVVHQYCLISSTHSTFQMPQKEDILKQRVVVVTLNTSQYLCQLDLEPGFFTHILLDEAAQAMECETIMPLALANKNTRIVLAGDHMQLSPFVYSEFARERNLHVSLLDRLYEHYPAEFPCRILLCENYRSHEAIINYTSELFYEGKLMASGKQPAHKDFYPLTFFTARGEDVQEKNSTAFYNNAEVFEVVERVEELRRKWPVAWGKLDDGSIGVVTPYADQVFRIRAELRKKRLSDVNVERVLNVQGKQFRVLFLSTVRTRHTCKHKQTPIKRKEQLLEDSTEDLDYGFLSNYKLLNTAITRAQSLVAVVGDPIALCSIGRCRKFWERFIALCHDNKSLHGITFEQIKTQLEALELKKTYVLNPLAPEFIPRALRQQHLGNTSKQQQSPPKGKVHHHNQNEHFQNEGIVQPNPSVLIGNPIRAYTPPSPLGPHPNMGKSPSPVQRIDPHTGTSILYVPAVYGGNVVMSVPLPVPWTGYQGRFAVDPRIITHQAAMAYNMNLLQTHGRGSPIPYGLGHHSPVSIGQQQIQHQEKDQHEQNRNGKSDTNPGPEINKIRTPEKKPLESKQNDLDSNPQNRSPETRPGVAYPNPKFHRKDNLNPRHLNLHLTAPHTQYAVPHRHYQHLSQLPRQPFPVQQPSSLNQQQNHFPEQLTQITPQPNQAVQQHNHLNQQTQQQPSQLSPTYQIGPSHSFFNNAVSHRPHSPAVDAVVSEQHPPSMLQEANSLRSIAQHNTMIPPTHLNNFIEESPPGLPLGEALERMHGNVALETLRQQQARIQQWSEHNAYLSQSSLPYPHYHHPHLQHLPQQPAGLHQQPVRANWKLASNAEDEAEATHSRFQDLLRELAHHDQSETRELTEMPPPQSRLLQYRQIQTRSPPANPSSSSNSNHSGQFSNFNDNIRDIEMASNPAFQQHLPPQIYNPSFSIPSENLTPPPLKYLPPDGSWTFANLHQNHLMGQGFHYGLPPLPHRPQQNSFIQIQNQHVVGQEPFHPLSSRVVSASSLHSLEEYEPRGPGRPLYQRRISSSSVPPCSEELNTPQDSHAQGKEHQDHNNQSSFNYSSPELWVNTTSSAPHQNIPCNGSSRTAPPRELLAPSKPLKSSEDNLKSENLQLSNSFNYSMLQHLGQFPPLMPNKQIVESNGNSQPSSGGNKPIMSYASALRAPPKPKPPPEQTKKKSDPLSLFQELSLGSSSGSNGFYSYFK

Target ORF information:

RefSeq Version	XM_031968638.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii helicase with zinc finger (HELZ), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031968638.1

ORF Insert Sequence:

ATGGACTACT CTACCCTGGT AGCTCCTCTT TGTGCTACTC TTAATGCCCG TTCGTCAACG 
GCACAACTAA ACCACACTTA CCTGTCTTCG GGCACCAACC CGGATCCCAT AGAGACAGAC 
CACCAGGAGC TTAGTTGGGC TTCACACCAA AGTTTCTGCA GACTTGGTTT TCCCTCCACC 
ACTCTTCGCT GTTTTGTGAG GGTATATTTC AGAAATCGCT GGGTAAAGAC TGTCCACCCA 
GTTGTACATC AATACTGTTT GATTTCAAGC ACACATTCCA CCTTTCAGAT GCCACAGAAA 
GAAGACATTC TTAAACAACG AGTTGTAGTT GTTACATTGA ATACTTCACA GTACCTTTGT 
CAACTGGATC TTGAACCTGG GTTTTTTACA CACATTCTTT TGGATGAAGC TGCACAGGCC 
ATGGAGTGTG AAACCATTAT GCCTCTGGCA TTAGCTAATA AAAACACACG AATTGTTTTG 
GCTGGAGACC ATATGCAACT CAGTCCTTTT GTCTACAGTG AGTTTGCCAG GGAGAGAAAC 
CTTCATGTTT CATTACTGGA TCGGCTCTAT GAACATTATC CTGCAGAGTT TCCATGTCGG 
ATCCTACTGT GTGAGAACTA TCGTTCCCAT GAAGCTATCA TCAATTACAC ATCAGAACTT 
TTCTATGAGG GGAAGTTGAT GGCAAGTGGA AAACAACCAG CACACAAAGA TTTTTATCCA 
TTAACCTTCT TTACAGCACG AGGAGAAGAT GTACAGGAAA AAAATAGTAC AGCCTTTTAT 
AATAATGCAG AGGTATTTGA AGTGGTGGAA CGGGTGGAAG AGTTAAGGAG AAAGTGGCCT 
GTAGCTTGGG GAAAATTAGA TGATGGAAGT ATAGGTGTAG TCACTCCTTA TGCTGATCAG 
GTGTTCAGGA TCCGGGCAGA ACTTCGAAAA AAGAGATTAT CTGATGTCAA TGTGGAAAGA 
GTGCTCAATG TGCAGGGAAA GCAGTTTAGG GTTTTGTTTC TGAGTACAGT ACGTACCCGG 
CACACTTGCA AACATAAACA AACCCCAATT AAAAGGAAAG AGCAACTGTT AGAAGATTCA 
ACAGAGGATT TGGATTATGG TTTTTTGTCC AACTACAAAC TTCTCAACAC CGCCATAACA 
AGAGCACAGT CCTTGGTAGC TGTGGTGGGT GATCCTATTG CGCTGTGCTC TATTGGAAGA 
TGCAGGAAAT TTTGGGAGCG GTTTATTGCC TTGTGTCATG ACAACAAAAG CCTACATGGT 
ATCACTTTTG AACAGATCAA AACACAGTTA GAGGCTTTGG AGCTAAAGAA AACATATGTG 
TTGAATCCAC TTGCTCCTGA ATTTATCCCT CGGGCTCTAA GACAGCAACA TTTGGGAAAT 
ACCAGCAAAC AGCAGCAATC ACCACCCAAG GGAAAAGTCC ACCATCATAA CCAAAATGAA 
CACTTCCAGA ATGAAGGAAT TGTTCAACCC AATCCTTCAG TACTCATTGG CAATCCTATT 
AGAGCATATA CACCTCCTTC CCCTCTTGGA CCTCACCCAA ATATGGGAAA GTCTCCTAGT 
CCTGTTCAAA GGATAGATCC GCACACTGGG ACTAGTATTC TTTATGTACC TGCTGTCTAT 
GGAGGAAATG TGGTTATGTC CGTGCCTTTA CCTGTACCAT GGACAGGGTA CCAGGGTAGG 
TTTGCAGTTG ACCCTCGAAT CATTACACAT CAGGCAGCGA TGGCATATAA TATGAACCTG 
TTGCAGACAC ATGGACGGGG GTCTCCTATA CCATATGGCC TTGGACATCA TTCACCAGTT 
AGTATAGGGC AACAGCAAAT TCAGCATCAG GAAAAGGATC AACATGAACA AAATCGAAAT 
GGTAAAAGTG ATACTAATCC TGGACCTGAA ATAAATAAAA TTCGTACTCC AGAGAAAAAG 
CCTCTAGAAT CAAAACAGAA TGATTTAGAT TCAAATCCAC AAAACCGAAG TCCAGAAACT 
CGCCCTGGTG TTGCTTATCC CAATCCCAAA TTTCATCGCA AAGATAATCT CAACCCAAGA 
CACCTAAACC TTCATCTTAC GGCTCCCCAC ACACAATATG CAGTCCCTCA TCGACATTAT 
CAGCACTTAT CCCAGCTACC AAGACAACCA TTTCCAGTTC AACAGCCCTC AAGTTTAAAT 
CAGCAGCAAA ATCATTTCCC TGAGCAACTG ACTCAAATAA CACCTCAACC AAACCAAGCA 
GTCCAGCAGC ATAACCACTT GAATCAACAA ACTCAGCAGC AACCCTCTCA ACTTTCTCCT 
ACATATCAAA TAGGTCCCAG TCATTCCTTC TTCAATAATG CAGTCTCTCA TCGACCACAT 
TCTCCTGCTG TAGATGCAGT AGTATCTGAA CAGCACCCCC CTTCGATGCT GCAAGAAGCT 
AACTCTCTGA GATCCATTGC ACAACACAAC ACGATGATAC CCCCCACTCA CTTGAATAAT 
TTTATTGAAG AAAGTCCTCC AGGATTACCA TTAGGAGAAG CTTTAGAACG TATGCATGGG 
AATGTGGCCT TAGAAACATT AAGGCAACAA CAAGCAAGAA TACAGCAGTG GAGTGAGCAT 
AATGCCTATC TCAGTCAAAG CAGTCTTCCA TATCCACACT ATCACCATCC TCACCTGCAG 
CATCTTCCCC AGCAACCTGC AGGATTACAT CAACAGCCAG TTAGAGCAAA CTGGAAACTT 
GCCAGTAATG CAGAAGATGA AGCAGAAGCA ACACATTCAA GGTTTCAAGA CTTACTCAGA 
GAGCTGGCTC ATCATGATCA AAGTGAAACA CGGGAGCTAA CTGAAATGCC ACCACCTCAG 
TCAAGACTTT TGCAATATAG ACAAATTCAA ACTAGAAGCC CACCAGCAAA CCCTTCTTCT 
TCATCTAATT CAAATCATAG TGGCCAATTT TCTAACTTCA ACGACAACAT TAGAGACATT 
GAAATGGCCA GCAATCCAGC ATTTCAGCAG CATCTGCCAC CCCAAATATA TAATCCCTCA 
TTTTCAATAC CATCTGAAAA CCTTACCCCT CCTCCTTTGA AATACCTGCC ACCAGATGGA 
TCCTGGACCT TTGCTAATTT ACATCAGAAT CACTTAATGG GACAGGGCTT TCACTATGGC 
CTACCACCAT TACCCCACAG ACCACAACAG AACTCTTTTA TACAAATTCA GAATCAACAT 
GTGGTTGGTC AGGAGCCATT TCACCCACTG TCATCTCGTG TGGTATCTGC TTCTTCGCTC 
CACAGCTTAG AAGAGTATGA ACCACGAGGA CCAGGCAGGC CATTGTACCA AAGAAGAATT 
TCATCTAGTT CAGTACCGCC TTGTTCTGAA GAGTTAAACA CTCCTCAAGA TAGTCATGCT 
CAGGGTAAAG AGCATCAGGA CCATAATAAT CAATCTTCTT TCAACTATTC ATCACCGGAG 
TTGTGGGTAA ATACCACCTC ATCTGCTCCA CATCAGAACA TTCCATGCAA TGGATCCAGC 
AGAACAGCCC CGCCCAGAGA ATTGTTAGCC CCATCAAAGC CCTTGAAATC CTCTGAGGAT 
AACCTGAAAT CTGAAAACCT TCAGCTGTCC AATTCCTTCA ACTACAGCAT GTTGCAGCAT 
CTAGGCCAGT TCCCACCCCT AATGCCCAAC AAGCAGATTG TGGAATCAAA CGGCAACAGC 
CAGCCAAGTT CTGGGGGGAA CAAGCCTATT ATGTCCTACG CAAGTGCTCT GCGGGCACCT 
CCAAAGCCCA AACCTCCACC TGAACAGACA AAAAAGAAGA GTGACCCTTT GTCTCTGTTT 
CAGGAACTTA GTTTAGGTAG CTCATCAGGT AGCAATGGCT TTTATTCCTA TTTTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELZ ( XM_003768578.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003768626.1 Sarcophilus harrisii helicase with zinc finger (HELZ), mRNA.

Price & Availability↑

CloneID	OSa130608
Clone ID Related Accession (Same CDS sequence)	XM_003768578.3 , XM_003768578.2
Accession Version	XM_003768578.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	probable helicase with zinc finger domain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838853.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_003768578.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGCAGACA GAAGACCTGA AAAGTCATGT GAACAAGCAT GTGAATCTCT TAAAAGACAG 
GACTATGAAG TGGCAGTGAG ACATTGCACA GAGGCCCTTC TTTCCCTTGG CCAGTATTCC 
TTGGCAAATT TCACAGAGAC ATGCCCATCA GAAATAGAAC GCATAAAAAT CGAGAGTCTT 
CTATATAGAA TTGCCTCATT TTTACAACTT AAGAACTATG GACAAGCTGA TGAAGACTGT 
AGGCATGTGT TGGGAGAGGG CTTGGCCAAG GGAGATGGCT CATTTCAAGC AGTGCTTTGT 
TGCATGCATC TTAAAGGAAA GCTCCAAGTT GTTTCTAATG TTCTTTCCAA GTCACTGATG 
GGAGAGTCTC TGAATGGAAT GGCAACAAAA GATTTGACAA GACTAAAGAC ACTTCTCACT 
GAAACAGAGA CAGCAACTAG TAAAGTGCCA TCTGAATATC ACGTGGAAGA CTTGGAAGAA 
GGTTCTCGGA ATGGTTGGCA TTTCCGTCCT CCACCTAGAG GAATCACCAG CAGTGAGGAG 
TATACTTTGT GTAAAAGATT CTTAGAACAA GGGATTTGTA GGTATGGTGC CCAGTGTACA 
TCAGCCCATT CCCAGGAAGA GCTATCTGAA TGGCAGAAAA GATATGCATC AAGACTTATA 
AAATTGAAAC AGCAGAAAGA AAGTAAACAG TTTTCTGGAA GTTACATGGA AACCTTGATA 
GAGAAATGGA TGAATTCACT AGCTCCTGAG AAAGTGCTTA GTGAATGTGT AGAAGGTGTG 
TGGGTGGAGC ATAATCCAGA ACTGTCGCTT ACTGTCACCA CCAAAAAATC TTATCAGACA 
TGGACATTTG CTCTCACTTG TAAGCCTGCC AGAATGCTGT ATCGAGTGGC ATTGCTTTAT 
GATGCTCATC GTCCTCATTT TAGCATCATT GGAATATCTG CTGGAGATAG TACTACTCAA 
GTATCACAGG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCTCAGAAT 
GGATTAGATC ATTATGTGTA TAAAATTGGG ATAGCATTTA ACACAGAGAT ATTTGGAACT 
TTTCGCCAAA CCATAGTTTT TGACTTTGGA TTGGAACCAG TGCTCATGCA AAGAGTAATG 
ATTGATGCTG CTTCTACAGA AGTACAAGCC AGGAAGATCC AAAGAAACAC CATAGGCTGG 
GAATTAATCT ATGTGAAGTG CAAATACCTC TGGGCTAGTT CTGCAGAAAC ACCAAGTGGG 
GACTCCTGGG GTGAGCTGGG TTTGGCTGTA CCTTCAGCAG TAACCACAGA AACTTTTACC 
TCCTGGACGG CGGTATATTT CAGAAATCGC TGGGTAAAGA CTGTCCACCC AGTTGTACAT 
CAATACTGTT TGATTTCAAG CACACATTCC ACCTTTCAGA TGCCACAGAA AGAAGACATT 
CTTAAACAAC GAGTTGTAGT TGTTACATTG AATACTTCAC AGTACCTTTG TCAACTGGAT 
CTTGAACCTG GGTTTTTTAC ACACATTCTT TTGGATGAAG CTGCACAGGC CATGGAGTGT 
GAAACCATTA TGCCTCTGGC ATTAGCTAAT AAAAACACAC GAATTGTTTT GGCTGGAGAC 
CATATGCAAC TCAGTCCTTT TGTCTACAGT GAGTTTGCCA GGGAGAGAAA CCTTCATGTT 
TCATTACTGG ATCGGCTCTA TGAACATTAT CCTGCAGAGT TTCCATGTCG GATCCTACTG 
TGTGAGAACT ATCGTTCCCA TGAAGCTATC ATCAATTACA CATCAGAACT TTTCTATGAG 
GGGAAGTTGA TGGCAAGTGG AAAACAACCA GCACACAAAG ATTTTTATCC ATTAACCTTC 
TTTACAGCAC GAGGAGAAGA TGTACAGGAA AAAAATAGTA CAGCCTTTTA TAATAATGCA 
GAGGTATTTG AAGTGGTGGA ACGGGTGGAA GAGTTAAGGA GAAAGTGGCC TGTAGCTTGG 
GGAAAATTAG ATGATGGAAG TATAGGTGTA GTCACTCCTT ATGCTGATCA GGTGTTCAGG 
ATCCGGGCAG AACTTCGAAA AAAGAGATTA TCTGATGTCA ATGTGGAAAG AGTGCTCAAT 
GTGCAGGGAA AGCAGTTTAG GGTTTTGTTT CTGAGTACAG TACGTACCCG GCACACTTGC 
AAACATAAAC AAACCCCAAT TAAAAGGAAA GAGCAACTGT TAGAAGATTC AACAGAGGAT 
TTGGATTATG GTTTTTTGTC CAACTACAAA CTTCTCAACA CCGCCATAAC AAGAGCACAG 
TCCTTGGTAG CTGTGGTGGG TGATCCTATT GCGCTGTGCT CTATTGGAAG ATGCAGGAAA 
TTTTGGGAGC GGTTTATTGC CTTGTGTCAT GACAACAAAA GCCTACATGG TATCACTTTT 
GAACAGATCA AAACACAGTT AGAGGCTTTG GAGCTAAAGA AAACATATGT GTTGAATCCA 
CTTGCTCCTG AATTTATCCC TCGGGCTCTA AGACAGCAAC ATTTGGGAAA TACCAGCAAA 
CAGCAGCAAT CACCACCCAA GGGAAAAGTC CACCATCATA ACCAAAATGA ACACTTCCAG 
AATGAAGGAA TTGTTCAACC CAATCCTTCA GTACTCATTG GCAATCCTAT TAGAGCATAT 
ACACCTCCTT CCCCTCTTGG ACCTCACCCA AATATGGGAA AGTCTCCTAG TCCTGTTCAA 
AGGATAGATC CGCACACTGG GACTAGTATT CTTTATGTAC CTGCTGTCTA TGGAGGAAAT 
GTGGTTATGT CCGTGCCTTT ACCTGTACCA TGGACAGGGT ACCAGGGTAG GTTTGCAGTT 
GACCCTCGAA TCATTACACA TCAGGCAGCG ATGGCATATA ATATGAACCT GTTGCAGACA 
CATGGACGGG GGTCTCCTAT ACCATATGGC CTTGGACATC ATTCACCAGT TAGTATAGGG 
CAACAGCAAA TTCAGCATCA GGAAAAGGAT CAACATGAAC AAAATCGAAA TGGTAAAAGT 
GATACTAATC CTGGACCTGA AATAAATAAA ATTCGTACTC CAGAGAAAAA GCCTCTAGAA 
TCAAAACAGA ATGATTTAGA TTCAAATCCA CAAAACCGAA GTCCAGAAAC TCGCCCTGGT 
GTTGCTTATC CCAATCCCAA ATTTCATCGC AAAGATAATC TCAACCCAAG ACACCTAAAC 
CTTCATCTTA CGGCTCCCCA CACACAATAT GCAGTCCCTC ATCGACATTA TCAGCACTTA 
TCCCAGCTAC CAAGACAACC ATTTCCAGTT CAACAGCCCT CAAGTTTAAA TCAGCAGCAA 
AATCATTTCC CTGAGCAACT GACTCAAATA ACACCTCAAC CAAACCAAGC AGTCCAGCAG 
CATAACCACT TGAATCAACA AACTCAGCAG CAACCCTCTC AACTTTCTCC TACATATCAA 
ATAGGTCCCA GTCATTCCTT CTTCAATAAT GCAGTCTCTC ATCGACCACA TTCTCCTGCT 
GTAGATGCAG TAGTATCTGA ACAGCACCCC CCTTCGATGC TGCAAGAAGC TAACTCTCTG 
AGATCCATTG CACAACACAA CACGATGATA CCCCCCACTC ACTTGAATAA TTTTATTGAA 
GAAAGTCCTC CAGGATTACC ATTAGGAGAA GCTTTAGAAC GTATGCATGG GAATGTGGCC 
TTAGAAACAT TAAGGCAACA ACAAGCAAGA ATACAGCAGT GGAGTGAGCA TAATGCCTAT 
CTCAGTCAAA GCAGTCTTCC ATATCCACAC TATCACCATC CTCACCTGCA GCATCTTCCC 
CAGCAACCTG CAGGATTACA TCAACAGCCA GTTAGAGCAA ACTGGAAACT TGCCAGTAAT 
GCAGAAGATG AAGCAGAAGC AACACATTCA AGGTTTCAAG ACTTACTCAG AGAGCTGGCT 
CATCATGATC AAAGTGAAAC ACGGGAGCTA ACTGAAATGC CACCACCTCA GTCAAGACTT 
TTGCAATATA GACAAATTCA AACTAGAAGC CCACCAGCAA ACCCTTCTTC TTCATCTAAT 
TCAAATCATA GTGGCCAATT TTCTAACTTC AACGACAACA TTAGAGACAT TGAAATGGCC 
AGCAATCCAG CATTTCAGCA GCATCTGCCA CCCCAAATAT ATAATCCCTC ATTTTCAATA 
CCATCTGAAA ACCTTACCCC TCCTCCTTTG AAATACCTGC CACCAGATGG ATCCTGGACC 
TTTGCTAATT TACATCAGAA TCACTTAATG GGACAGGGCT TTCACTATGG CCTACCACCA 
TTACCCCACA GACCACAACA GAACTCTTTT ATACAAATTC AGAATCAACA TGTGGTTGGT 
CAGGAGCCAT TTCACCCACT GTCATCTCGT GTGGTATCTG CTTCTTCGCT CCACAGCTTA 
GAAGAGTATG AACCACGAGG ACCAGGCAGG CCATTGTACC AAAGAAGAAT TTCATCTAGT 
TCAGTACCGC CTTGTTCTGA AGAGTTAAAC ACTCCTCAAG ATAGTCATGC TCAGGGTAAA 
GAGCATCAGG ACCATAATAA TCAATCTTCT TTCAACTATT CATCACCGGA GTTGTGGGTA 
AATACCACCT CATCTGCTCC ACATCAGAAC ATTCCATGCA ATGGATCCAG CAGAACAGCC 
CCGCCCAGAG AATTGTTAGC CCCATCAAAG CCCTTGAAAT CCTCTGAGGA TAACCTGAAA 
TCTGAAAACC TTCAGCTGTC CAATTCCTTC AACTACAGCA TGTTGCAGCA TCTAGGCCAG 
TTCCCACCCC TAATGCCCAA CAAGCAGATT GTGGAATCAA ACGGCAACAG CCAGCCAAGT 
TCTGGGGGGA ACAAGCCTAT TATGTCCTAC GCAAGTGCTC TGCGGGCACC TCCAAAGCCC 
AAACCTCCAC CTGAACAGAC AAAAAAGAAG AGTGACCCTT TGTCTCTGTT TCAGGAACTT 
AGTTTAGGTA GCTCATCAGG TAGCAATGGC TTTTATTCCT ATTTTAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003768626.1
CDS	60..5030
Translation	MADRRPEKSCEQACESLKRQDYEVAVRHCTEALLSLGQYSLANFTETCPSEIERIKIESLLYRIASFLQLKNYGQADEDCRHVLGEGLAKGDGSFQAVLCCMHLKGKLQVVSNVLSKSLMGESLNGMATKDLTRLKTLLTETETATSKVPSEYHVEDLEEGSRNGWHFRPPPRGITSSEEYTLCKRFLEQGICRYGAQCTSAHSQEELSEWQKRYASRLIKLKQQKESKQFSGSYMETLIEKWMNSLAPEKVLSECVEGVWVEHNPELSLTVTTKKSYQTWTFALTCKPARMLYRVALLYDAHRPHFSIIGISAGDSTTQVSQEVPENCQEWIGGKMAQNGLDHYVYKIGIAFNTEIFGTFRQTIVFDFGLEPVLMQRVMIDAASTEVQARKIQRNTIGWELIYVKCKYLWASSAETPSGDSWGELGLAVPSAVTTETFTSWTAVYFRNRWVKTVHPVVHQYCLISSTHSTFQMPQKEDILKQRVVVVTLNTSQYLCQLDLEPGFFTHILLDEAAQAMECETIMPLALANKNTRIVLAGDHMQLSPFVYSEFARERNLHVSLLDRLYEHYPAEFPCRILLCENYRSHEAIINYTSELFYEGKLMASGKQPAHKDFYPLTFFTARGEDVQEKNSTAFYNNAEVFEVVERVEELRRKWPVAWGKLDDGSIGVVTPYADQVFRIRAELRKKRLSDVNVERVLNVQGKQFRVLFLSTVRTRHTCKHKQTPIKRKEQLLEDSTEDLDYGFLSNYKLLNTAITRAQSLVAVVGDPIALCSIGRCRKFWERFIALCHDNKSLHGITFEQIKTQLEALELKKTYVLNPLAPEFIPRALRQQHLGNTSKQQQSPPKGKVHHHNQNEHFQNEGIVQPNPSVLIGNPIRAYTPPSPLGPHPNMGKSPSPVQRIDPHTGTSILYVPAVYGGNVVMSVPLPVPWTGYQGRFAVDPRIITHQAAMAYNMNLLQTHGRGSPIPYGLGHHSPVSIGQQQIQHQEKDQHEQNRNGKSDTNPGPEINKIRTPEKKPLESKQNDLDSNPQNRSPETRPGVAYPNPKFHRKDNLNPRHLNLHLTAPHTQYAVPHRHYQHLSQLPRQPFPVQQPSSLNQQQNHFPEQLTQITPQPNQAVQQHNHLNQQTQQQPSQLSPTYQIGPSHSFFNNAVSHRPHSPAVDAVVSEQHPPSMLQEANSLRSIAQHNTMIPPTHLNNFIEESPPGLPLGEALERMHGNVALETLRQQQARIQQWSEHNAYLSQSSLPYPHYHHPHLQHLPQQPAGLHQQPVRANWKLASNAEDEAEATHSRFQDLLRELAHHDQSETRELTEMPPPQSRLLQYRQIQTRSPPANPSSSSNSNHSGQFSNFNDNIRDIEMASNPAFQQHLPPQIYNPSFSIPSENLTPPPLKYLPPDGSWTFANLHQNHLMGQGFHYGLPPLPHRPQQNSFIQIQNQHVVGQEPFHPLSSRVVSASSLHSLEEYEPRGPGRPLYQRRISSSSVPPCSEELNTPQDSHAQGKEHQDHNNQSSFNYSSPELWVNTTSSAPHQNIPCNGSSRTAPPRELLAPSKPLKSSEDNLKSENLQLSNSFNYSMLQHLGQFPPLMPNKQIVESNGNSQPSSGGNKPIMSYASALRAPPKPKPPPEQTKKKSDPLSLFQELSLGSSSGSNGFYSYFK

Target ORF information:

RefSeq Version	XM_003768578.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii helicase with zinc finger (HELZ), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003768578.3

ORF Insert Sequence:

ATGGCAGACA GAAGACCTGA AAAGTCATGT GAACAAGCAT GTGAATCTCT TAAAAGACAG 
GACTATGAAG TGGCAGTGAG ACATTGCACA GAGGCCCTTC TTTCCCTTGG CCAGTATTCC 
TTGGCAAATT TCACAGAGAC ATGCCCATCA GAAATAGAAC GCATAAAAAT CGAGAGTCTT 
CTATATAGAA TTGCCTCATT TTTACAACTT AAGAACTATG GACAAGCTGA TGAAGACTGT 
AGGCATGTGT TGGGAGAGGG CTTGGCCAAG GGAGATGGCT CATTTCAAGC AGTGCTTTGT 
TGCATGCATC TTAAAGGAAA GCTCCAAGTT GTTTCTAATG TTCTTTCCAA GTCACTGATG 
GGAGAGTCTC TGAATGGAAT GGCAACAAAA GATTTGACAA GACTAAAGAC ACTTCTCACT 
GAAACAGAGA CAGCAACTAG TAAAGTGCCA TCTGAATATC ACGTGGAAGA CTTGGAAGAA 
GGTTCTCGGA ATGGTTGGCA TTTCCGTCCT CCACCTAGAG GAATCACCAG CAGTGAGGAG 
TATACTTTGT GTAAAAGATT CTTAGAACAA GGGATTTGTA GGTATGGTGC CCAGTGTACA 
TCAGCCCATT CCCAGGAAGA GCTATCTGAA TGGCAGAAAA GATATGCATC AAGACTTATA 
AAATTGAAAC AGCAGAAAGA AAGTAAACAG TTTTCTGGAA GTTACATGGA AACCTTGATA 
GAGAAATGGA TGAATTCACT AGCTCCTGAG AAAGTGCTTA GTGAATGTGT AGAAGGTGTG 
TGGGTGGAGC ATAATCCAGA ACTGTCGCTT ACTGTCACCA CCAAAAAATC TTATCAGACA 
TGGACATTTG CTCTCACTTG TAAGCCTGCC AGAATGCTGT ATCGAGTGGC ATTGCTTTAT 
GATGCTCATC GTCCTCATTT TAGCATCATT GGAATATCTG CTGGAGATAG TACTACTCAA 
GTATCACAGG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCTCAGAAT 
GGATTAGATC ATTATGTGTA TAAAATTGGG ATAGCATTTA ACACAGAGAT ATTTGGAACT 
TTTCGCCAAA CCATAGTTTT TGACTTTGGA TTGGAACCAG TGCTCATGCA AAGAGTAATG 
ATTGATGCTG CTTCTACAGA AGTACAAGCC AGGAAGATCC AAAGAAACAC CATAGGCTGG 
GAATTAATCT ATGTGAAGTG CAAATACCTC TGGGCTAGTT CTGCAGAAAC ACCAAGTGGG 
GACTCCTGGG GTGAGCTGGG TTTGGCTGTA CCTTCAGCAG TAACCACAGA AACTTTTACC 
TCCTGGACGG CGGTATATTT CAGAAATCGC TGGGTAAAGA CTGTCCACCC AGTTGTACAT 
CAATACTGTT TGATTTCAAG CACACATTCC ACCTTTCAGA TGCCACAGAA AGAAGACATT 
CTTAAACAAC GAGTTGTAGT TGTTACATTG AATACTTCAC AGTACCTTTG TCAACTGGAT 
CTTGAACCTG GGTTTTTTAC ACACATTCTT TTGGATGAAG CTGCACAGGC CATGGAGTGT 
GAAACCATTA TGCCTCTGGC ATTAGCTAAT AAAAACACAC GAATTGTTTT GGCTGGAGAC 
CATATGCAAC TCAGTCCTTT TGTCTACAGT GAGTTTGCCA GGGAGAGAAA CCTTCATGTT 
TCATTACTGG ATCGGCTCTA TGAACATTAT CCTGCAGAGT TTCCATGTCG GATCCTACTG 
TGTGAGAACT ATCGTTCCCA TGAAGCTATC ATCAATTACA CATCAGAACT TTTCTATGAG 
GGGAAGTTGA TGGCAAGTGG AAAACAACCA GCACACAAAG ATTTTTATCC ATTAACCTTC 
TTTACAGCAC GAGGAGAAGA TGTACAGGAA AAAAATAGTA CAGCCTTTTA TAATAATGCA 
GAGGTATTTG AAGTGGTGGA ACGGGTGGAA GAGTTAAGGA GAAAGTGGCC TGTAGCTTGG 
GGAAAATTAG ATGATGGAAG TATAGGTGTA GTCACTCCTT ATGCTGATCA GGTGTTCAGG 
ATCCGGGCAG AACTTCGAAA AAAGAGATTA TCTGATGTCA ATGTGGAAAG AGTGCTCAAT 
GTGCAGGGAA AGCAGTTTAG GGTTTTGTTT CTGAGTACAG TACGTACCCG GCACACTTGC 
AAACATAAAC AAACCCCAAT TAAAAGGAAA GAGCAACTGT TAGAAGATTC AACAGAGGAT 
TTGGATTATG GTTTTTTGTC CAACTACAAA CTTCTCAACA CCGCCATAAC AAGAGCACAG 
TCCTTGGTAG CTGTGGTGGG TGATCCTATT GCGCTGTGCT CTATTGGAAG ATGCAGGAAA 
TTTTGGGAGC GGTTTATTGC CTTGTGTCAT GACAACAAAA GCCTACATGG TATCACTTTT 
GAACAGATCA AAACACAGTT AGAGGCTTTG GAGCTAAAGA AAACATATGT GTTGAATCCA 
CTTGCTCCTG AATTTATCCC TCGGGCTCTA AGACAGCAAC ATTTGGGAAA TACCAGCAAA 
CAGCAGCAAT CACCACCCAA GGGAAAAGTC CACCATCATA ACCAAAATGA ACACTTCCAG 
AATGAAGGAA TTGTTCAACC CAATCCTTCA GTACTCATTG GCAATCCTAT TAGAGCATAT 
ACACCTCCTT CCCCTCTTGG ACCTCACCCA AATATGGGAA AGTCTCCTAG TCCTGTTCAA 
AGGATAGATC CGCACACTGG GACTAGTATT CTTTATGTAC CTGCTGTCTA TGGAGGAAAT 
GTGGTTATGT CCGTGCCTTT ACCTGTACCA TGGACAGGGT ACCAGGGTAG GTTTGCAGTT 
GACCCTCGAA TCATTACACA TCAGGCAGCG ATGGCATATA ATATGAACCT GTTGCAGACA 
CATGGACGGG GGTCTCCTAT ACCATATGGC CTTGGACATC ATTCACCAGT TAGTATAGGG 
CAACAGCAAA TTCAGCATCA GGAAAAGGAT CAACATGAAC AAAATCGAAA TGGTAAAAGT 
GATACTAATC CTGGACCTGA AATAAATAAA ATTCGTACTC CAGAGAAAAA GCCTCTAGAA 
TCAAAACAGA ATGATTTAGA TTCAAATCCA CAAAACCGAA GTCCAGAAAC TCGCCCTGGT 
GTTGCTTATC CCAATCCCAA ATTTCATCGC AAAGATAATC TCAACCCAAG ACACCTAAAC 
CTTCATCTTA CGGCTCCCCA CACACAATAT GCAGTCCCTC ATCGACATTA TCAGCACTTA 
TCCCAGCTAC CAAGACAACC ATTTCCAGTT CAACAGCCCT CAAGTTTAAA TCAGCAGCAA 
AATCATTTCC CTGAGCAACT GACTCAAATA ACACCTCAAC CAAACCAAGC AGTCCAGCAG 
CATAACCACT TGAATCAACA AACTCAGCAG CAACCCTCTC AACTTTCTCC TACATATCAA 
ATAGGTCCCA GTCATTCCTT CTTCAATAAT GCAGTCTCTC ATCGACCACA TTCTCCTGCT 
GTAGATGCAG TAGTATCTGA ACAGCACCCC CCTTCGATGC TGCAAGAAGC TAACTCTCTG 
AGATCCATTG CACAACACAA CACGATGATA CCCCCCACTC ACTTGAATAA TTTTATTGAA 
GAAAGTCCTC CAGGATTACC ATTAGGAGAA GCTTTAGAAC GTATGCATGG GAATGTGGCC 
TTAGAAACAT TAAGGCAACA ACAAGCAAGA ATACAGCAGT GGAGTGAGCA TAATGCCTAT 
CTCAGTCAAA GCAGTCTTCC ATATCCACAC TATCACCATC CTCACCTGCA GCATCTTCCC 
CAGCAACCTG CAGGATTACA TCAACAGCCA GTTAGAGCAA ACTGGAAACT TGCCAGTAAT 
GCAGAAGATG AAGCAGAAGC AACACATTCA AGGTTTCAAG ACTTACTCAG AGAGCTGGCT 
CATCATGATC AAAGTGAAAC ACGGGAGCTA ACTGAAATGC CACCACCTCA GTCAAGACTT 
TTGCAATATA GACAAATTCA AACTAGAAGC CCACCAGCAA ACCCTTCTTC TTCATCTAAT 
TCAAATCATA GTGGCCAATT TTCTAACTTC AACGACAACA TTAGAGACAT TGAAATGGCC 
AGCAATCCAG CATTTCAGCA GCATCTGCCA CCCCAAATAT ATAATCCCTC ATTTTCAATA 
CCATCTGAAA ACCTTACCCC TCCTCCTTTG AAATACCTGC CACCAGATGG ATCCTGGACC 
TTTGCTAATT TACATCAGAA TCACTTAATG GGACAGGGCT TTCACTATGG CCTACCACCA 
TTACCCCACA GACCACAACA GAACTCTTTT ATACAAATTC AGAATCAACA TGTGGTTGGT 
CAGGAGCCAT TTCACCCACT GTCATCTCGT GTGGTATCTG CTTCTTCGCT CCACAGCTTA 
GAAGAGTATG AACCACGAGG ACCAGGCAGG CCATTGTACC AAAGAAGAAT TTCATCTAGT 
TCAGTACCGC CTTGTTCTGA AGAGTTAAAC ACTCCTCAAG ATAGTCATGC TCAGGGTAAA 
GAGCATCAGG ACCATAATAA TCAATCTTCT TTCAACTATT CATCACCGGA GTTGTGGGTA 
AATACCACCT CATCTGCTCC ACATCAGAAC ATTCCATGCA ATGGATCCAG CAGAACAGCC 
CCGCCCAGAG AATTGTTAGC CCCATCAAAG CCCTTGAAAT CCTCTGAGGA TAACCTGAAA 
TCTGAAAACC TTCAGCTGTC CAATTCCTTC AACTACAGCA TGTTGCAGCA TCTAGGCCAG 
TTCCCACCCC TAATGCCCAA CAAGCAGATT GTGGAATCAA ACGGCAACAG CCAGCCAAGT 
TCTGGGGGGA ACAAGCCTAT TATGTCCTAC GCAAGTGCTC TGCGGGCACC TCCAAAGCCC 
AAACCTCCAC CTGAACAGAC AAAAAAGAAG AGTGACCCTT TGTCTCTGTT TCAGGAACTT 
AGTTTAGGTA GCTCATCAGG TAGCAATGGC TTTTATTCCT ATTTTAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELZ ( XM_003768578.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003768626.1 Sarcophilus harrisii helicase with zinc finger (HELZ), mRNA.

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CloneID	OSa130608
Clone ID Related Accession (Same CDS sequence)	XM_003768578.3 , XM_003768578.2
Accession Version	XM_003768578.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	probable helicase with zinc finger domain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838853.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_003768578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGCAGACA GAAGACCTGA AAAGTCATGT GAACAAGCAT GTGAATCTCT TAAAAGACAG 
GACTATGAAG TGGCAGTGAG ACATTGCACA GAGGCCCTTC TTTCCCTTGG CCAGTATTCC 
TTGGCAAATT TCACAGAGAC ATGCCCATCA GAAATAGAAC GCATAAAAAT CGAGAGTCTT 
CTATATAGAA TTGCCTCATT TTTACAACTT AAGAACTATG GACAAGCTGA TGAAGACTGT 
AGGCATGTGT TGGGAGAGGG CTTGGCCAAG GGAGATGGCT CATTTCAAGC AGTGCTTTGT 
TGCATGCATC TTAAAGGAAA GCTCCAAGTT GTTTCTAATG TTCTTTCCAA GTCACTGATG 
GGAGAGTCTC TGAATGGAAT GGCAACAAAA GATTTGACAA GACTAAAGAC ACTTCTCACT 
GAAACAGAGA CAGCAACTAG TAAAGTGCCA TCTGAATATC ACGTGGAAGA CTTGGAAGAA 
GGTTCTCGGA ATGGTTGGCA TTTCCGTCCT CCACCTAGAG GAATCACCAG CAGTGAGGAG 
TATACTTTGT GTAAAAGATT CTTAGAACAA GGGATTTGTA GGTATGGTGC CCAGTGTACA 
TCAGCCCATT CCCAGGAAGA GCTATCTGAA TGGCAGAAAA GATATGCATC AAGACTTATA 
AAATTGAAAC AGCAGAAAGA AAGTAAACAG TTTTCTGGAA GTTACATGGA AACCTTGATA 
GAGAAATGGA TGAATTCACT AGCTCCTGAG AAAGTGCTTA GTGAATGTGT AGAAGGTGTG 
TGGGTGGAGC ATAATCCAGA ACTGTCGCTT ACTGTCACCA CCAAAAAATC TTATCAGACA 
TGGACATTTG CTCTCACTTG TAAGCCTGCC AGAATGCTGT ATCGAGTGGC ATTGCTTTAT 
GATGCTCATC GTCCTCATTT TAGCATCATT GGAATATCTG CTGGAGATAG TACTACTCAA 
GTATCACAGG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCTCAGAAT 
GGATTAGATC ATTATGTGTA TAAAATTGGG ATAGCATTTA ACACAGAGAT ATTTGGAACT 
TTTCGCCAAA CCATAGTTTT TGACTTTGGA TTGGAACCAG TGCTCATGCA AAGAGTAATG 
ATTGATGCTG CTTCTACAGA AGTACAAGCC AGGAAGATCC AAAGAAACAC CATAGGCTGG 
GAATTAATCT ATGTGAAGTG CAAATACCTC TGGGCTAGTT CTGCAGAAAC ACCAAGTGGG 
GACTCCTGGG GTGAGCTGGG TTTGGCTGTA CCTTCAGCAG TAACCACAGA AACTTTTACC 
TCCTGGACGG CGGTATATTT CAGAAATCGC TGGGTAAAGA CTGTCCACCC AGTTGTACAT 
CAATACTGTT TGATTTCAAG CACACATTCC ACCTTTCAGA TGCCACAGAA AGAAGACATT 
CTTAAACAAC GAGTTGTAGT TGTTACATTG AATACTTCAC AGTACCTTTG TCAACTGGAT 
CTTGAACCTG GGTTTTTTAC ACACATTCTT TTGGATGAAG CTGCACAGGC CATGGAGTGT 
GAAACCATTA TGCCTCTGGC ATTAGCTAAT AAAAACACAC GAATTGTTTT GGCTGGAGAC 
CATATGCAAC TCAGTCCTTT TGTCTACAGT GAGTTTGCCA GGGAGAGAAA CCTTCATGTT 
TCATTACTGG ATCGGCTCTA TGAACATTAT CCTGCAGAGT TTCCATGTCG GATCCTACTG 
TGTGAGAACT ATCGTTCCCA TGAAGCTATC ATCAATTACA CATCAGAACT TTTCTATGAG 
GGGAAGTTGA TGGCAAGTGG AAAACAACCA GCACACAAAG ATTTTTATCC ATTAACCTTC 
TTTACAGCAC GAGGAGAAGA TGTACAGGAA AAAAATAGTA CAGCCTTTTA TAATAATGCA 
GAGGTATTTG AAGTGGTGGA ACGGGTGGAA GAGTTAAGGA GAAAGTGGCC TGTAGCTTGG 
GGAAAATTAG ATGATGGAAG TATAGGTGTA GTCACTCCTT ATGCTGATCA GGTGTTCAGG 
ATCCGGGCAG AACTTCGAAA AAAGAGATTA TCTGATGTCA ATGTGGAAAG AGTGCTCAAT 
GTGCAGGGAA AGCAGTTTAG GGTTTTGTTT CTGAGTACAG TACGTACCCG GCACACTTGC 
AAACATAAAC AAACCCCAAT TAAAAGGAAA GAGCAACTGT TAGAAGATTC AACAGAGGAT 
TTGGATTATG GTTTTTTGTC CAACTACAAA CTTCTCAACA CCGCCATAAC AAGAGCACAG 
TCCTTGGTAG CTGTGGTGGG TGATCCTATT GCGCTGTGCT CTATTGGAAG ATGCAGGAAA 
TTTTGGGAGC GGTTTATTGC CTTGTGTCAT GACAACAAAA GCCTACATGG TATCACTTTT 
GAACAGATCA AAACACAGTT AGAGGCTTTG GAGCTAAAGA AAACATATGT GTTGAATCCA 
CTTGCTCCTG AATTTATCCC TCGGGCTCTA AGACAGCAAC ATTTGGGAAA TACCAGCAAA 
CAGCAGCAAT CACCACCCAA GGGAAAAGTC CACCATCATA ACCAAAATGA ACACTTCCAG 
AATGAAGGAA TTGTTCAACC CAATCCTTCA GTACTCATTG GCAATCCTAT TAGAGCATAT 
ACACCTCCTT CCCCTCTTGG ACCTCACCCA AATATGGGAA AGTCTCCTAG TCCTGTTCAA 
AGGATAGATC CGCACACTGG GACTAGTATT CTTTATGTAC CTGCTGTCTA TGGAGGAAAT 
GTGGTTATGT CCGTGCCTTT ACCTGTACCA TGGACAGGGT ACCAGGGTAG GTTTGCAGTT 
GACCCTCGAA TCATTACACA TCAGGCAGCG ATGGCATATA ATATGAACCT GTTGCAGACA 
CATGGACGGG GGTCTCCTAT ACCATATGGC CTTGGACATC ATTCACCAGT TAGTATAGGG 
CAACAGCAAA TTCAGCATCA GGAAAAGGAT CAACATGAAC AAAATCGAAA TGGTAAAAGT 
GATACTAATC CTGGACCTGA AATAAATAAA ATTCGTACTC CAGAGAAAAA GCCTCTAGAA 
TCAAAACAGA ATGATTTAGA TTCAAATCCA CAAAACCGAA GTCCAGAAAC TCGCCCTGGT 
GTTGCTTATC CCAATCCCAA ATTTCATCGC AAAGATAATC TCAACCCAAG ACACCTAAAC 
CTTCATCTTA CGGCTCCCCA CACACAATAT GCAGTCCCTC ATCGACATTA TCAGCACTTA 
TCCCAGCTAC CAAGACAACC ATTTCCAGTT CAACAGCCCT CAAGTTTAAA TCAGCAGCAA 
AATCATTTCC CTGAGCAACT GACTCAAATA ACACCTCAAC CAAACCAAGC AGTCCAGCAG 
CATAACCACT TGAATCAACA AACTCAGCAG CAACCCTCTC AACTTTCTCC TACATATCAA 
ATAGGTCCCA GTCATTCCTT CTTCAATAAT GCAGTCTCTC ATCGACCACA TTCTCCTGCT 
GTAGATGCAG TAGTATCTGA ACAGCACCCC CCTTCGATGC TGCAAGAAGC TAACTCTCTG 
AGATCCATTG CACAACACAA CACGATGATA CCCCCCACTC ACTTGAATAA TTTTATTGAA 
GAAAGTCCTC CAGGATTACC ATTAGGAGAA GCTTTAGAAC GTATGCATGG GAATGTGGCC 
TTAGAAACAT TAAGGCAACA ACAAGCAAGA ATACAGCAGT GGAGTGAGCA TAATGCCTAT 
CTCAGTCAAA GCAGTCTTCC ATATCCACAC TATCACCATC CTCACCTGCA GCATCTTCCC 
CAGCAACCTG CAGGATTACA TCAACAGCCA GTTAGAGCAA ACTGGAAACT TGCCAGTAAT 
GCAGAAGATG AAGCAGAAGC AACACATTCA AGGTTTCAAG ACTTACTCAG AGAGCTGGCT 
CATCATGATC AAAGTGAAAC ACGGGAGCTA ACTGAAATGC CACCACCTCA GTCAAGACTT 
TTGCAATATA GACAAATTCA AACTAGAAGC CCACCAGCAA ACCCTTCTTC TTCATCTAAT 
TCAAATCATA GTGGCCAATT TTCTAACTTC AACGACAACA TTAGAGACAT TGAAATGGCC 
AGCAATCCAG CATTTCAGCA GCATCTGCCA CCCCAAATAT ATAATCCCTC ATTTTCAATA 
CCATCTGAAA ACCTTACCCC TCCTCCTTTG AAATACCTGC CACCAGATGG ATCCTGGACC 
TTTGCTAATT TACATCAGAA TCACTTAATG GGACAGGGCT TTCACTATGG CCTACCACCA 
TTACCCCACA GACCACAACA GAACTCTTTT ATACAAATTC AGAATCAACA TGTGGTTGGT 
CAGGAGCCAT TTCACCCACT GTCATCTCGT GTGGTATCTG CTTCTTCGCT CCACAGCTTA 
GAAGAGTATG AACCACGAGG ACCAGGCAGG CCATTGTACC AAAGAAGAAT TTCATCTAGT 
TCAGTACCGC CTTGTTCTGA AGAGTTAAAC ACTCCTCAAG ATAGTCATGC TCAGGGTAAA 
GAGCATCAGG ACCATAATAA TCAATCTTCT TTCAACTATT CATCACCGGA GTTGTGGGTA 
AATACCACCT CATCTGCTCC ACATCAGAAC ATTCCATGCA ATGGATCCAG CAGAACAGCC 
CCGCCCAGAG AATTGTTAGC CCCATCAAAG CCCTTGAAAT CCTCTGAGGA TAACCTGAAA 
TCTGAAAACC TTCAGCTGTC CAATTCCTTC AACTACAGCA TGTTGCAGCA TCTAGGCCAG 
TTCCCACCCC TAATGCCCAA CAAGCAGATT GTGGAATCAA ACGGCAACAG CCAGCCAAGT 
TCTGGGGGGA ACAAGCCTAT TATGTCCTAC GCAAGTGCTC TGCGGGCACC TCCAAAGCCC 
AAACCTCCAC CTGAACAGAC AAAAAAGAAG AGTGACCCTT TGTCTCTGTT TCAGGAACTT 
AGTTTAGGTA GCTCATCAGG TAGCAATGGC TTTTATTCCT ATTTTAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003768626.1
CDS	15..4985
Translation	MADRRPEKSCEQACESLKRQDYEVAVRHCTEALLSLGQYSLANFTETCPSEIERIKIESLLYRIASFLQLKNYGQADEDCRHVLGEGLAKGDGSFQAVLCCMHLKGKLQVVSNVLSKSLMGESLNGMATKDLTRLKTLLTETETATSKVPSEYHVEDLEEGSRNGWHFRPPPRGITSSEEYTLCKRFLEQGICRYGAQCTSAHSQEELSEWQKRYASRLIKLKQQKESKQFSGSYMETLIEKWMNSLAPEKVLSECVEGVWVEHNPELSLTVTTKKSYQTWTFALTCKPARMLYRVALLYDAHRPHFSIIGISAGDSTTQVSQEVPENCQEWIGGKMAQNGLDHYVYKIGIAFNTEIFGTFRQTIVFDFGLEPVLMQRVMIDAASTEVQARKIQRNTIGWELIYVKCKYLWASSAETPSGDSWGELGLAVPSAVTTETFTSWTAVYFRNRWVKTVHPVVHQYCLISSTHSTFQMPQKEDILKQRVVVVTLNTSQYLCQLDLEPGFFTHILLDEAAQAMECETIMPLALANKNTRIVLAGDHMQLSPFVYSEFARERNLHVSLLDRLYEHYPAEFPCRILLCENYRSHEAIINYTSELFYEGKLMASGKQPAHKDFYPLTFFTARGEDVQEKNSTAFYNNAEVFEVVERVEELRRKWPVAWGKLDDGSIGVVTPYADQVFRIRAELRKKRLSDVNVERVLNVQGKQFRVLFLSTVRTRHTCKHKQTPIKRKEQLLEDSTEDLDYGFLSNYKLLNTAITRAQSLVAVVGDPIALCSIGRCRKFWERFIALCHDNKSLHGITFEQIKTQLEALELKKTYVLNPLAPEFIPRALRQQHLGNTSKQQQSPPKGKVHHHNQNEHFQNEGIVQPNPSVLIGNPIRAYTPPSPLGPHPNMGKSPSPVQRIDPHTGTSILYVPAVYGGNVVMSVPLPVPWTGYQGRFAVDPRIITHQAAMAYNMNLLQTHGRGSPIPYGLGHHSPVSIGQQQIQHQEKDQHEQNRNGKSDTNPGPEINKIRTPEKKPLESKQNDLDSNPQNRSPETRPGVAYPNPKFHRKDNLNPRHLNLHLTAPHTQYAVPHRHYQHLSQLPRQPFPVQQPSSLNQQQNHFPEQLTQITPQPNQAVQQHNHLNQQTQQQPSQLSPTYQIGPSHSFFNNAVSHRPHSPAVDAVVSEQHPPSMLQEANSLRSIAQHNTMIPPTHLNNFIEESPPGLPLGEALERMHGNVALETLRQQQARIQQWSEHNAYLSQSSLPYPHYHHPHLQHLPQQPAGLHQQPVRANWKLASNAEDEAEATHSRFQDLLRELAHHDQSETRELTEMPPPQSRLLQYRQIQTRSPPANPSSSSNSNHSGQFSNFNDNIRDIEMASNPAFQQHLPPQIYNPSFSIPSENLTPPPLKYLPPDGSWTFANLHQNHLMGQGFHYGLPPLPHRPQQNSFIQIQNQHVVGQEPFHPLSSRVVSASSLHSLEEYEPRGPGRPLYQRRISSSSVPPCSEELNTPQDSHAQGKEHQDHNNQSSFNYSSPELWVNTTSSAPHQNIPCNGSSRTAPPRELLAPSKPLKSSEDNLKSENLQLSNSFNYSMLQHLGQFPPLMPNKQIVESNGNSQPSSGGNKPIMSYASALRAPPKPKPPPEQTKKKSDPLSLFQELSLGSSSGSNGFYSYFK

Target ORF information:

RefSeq Version	XM_003768578.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii helicase with zinc finger (HELZ), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003768578.2

ORF Insert Sequence:

ATGGCAGACA GAAGACCTGA AAAGTCATGT GAACAAGCAT GTGAATCTCT TAAAAGACAG 
GACTATGAAG TGGCAGTGAG ACATTGCACA GAGGCCCTTC TTTCCCTTGG CCAGTATTCC 
TTGGCAAATT TCACAGAGAC ATGCCCATCA GAAATAGAAC GCATAAAAAT CGAGAGTCTT 
CTATATAGAA TTGCCTCATT TTTACAACTT AAGAACTATG GACAAGCTGA TGAAGACTGT 
AGGCATGTGT TGGGAGAGGG CTTGGCCAAG GGAGATGGCT CATTTCAAGC AGTGCTTTGT 
TGCATGCATC TTAAAGGAAA GCTCCAAGTT GTTTCTAATG TTCTTTCCAA GTCACTGATG 
GGAGAGTCTC TGAATGGAAT GGCAACAAAA GATTTGACAA GACTAAAGAC ACTTCTCACT 
GAAACAGAGA CAGCAACTAG TAAAGTGCCA TCTGAATATC ACGTGGAAGA CTTGGAAGAA 
GGTTCTCGGA ATGGTTGGCA TTTCCGTCCT CCACCTAGAG GAATCACCAG CAGTGAGGAG 
TATACTTTGT GTAAAAGATT CTTAGAACAA GGGATTTGTA GGTATGGTGC CCAGTGTACA 
TCAGCCCATT CCCAGGAAGA GCTATCTGAA TGGCAGAAAA GATATGCATC AAGACTTATA 
AAATTGAAAC AGCAGAAAGA AAGTAAACAG TTTTCTGGAA GTTACATGGA AACCTTGATA 
GAGAAATGGA TGAATTCACT AGCTCCTGAG AAAGTGCTTA GTGAATGTGT AGAAGGTGTG 
TGGGTGGAGC ATAATCCAGA ACTGTCGCTT ACTGTCACCA CCAAAAAATC TTATCAGACA 
TGGACATTTG CTCTCACTTG TAAGCCTGCC AGAATGCTGT ATCGAGTGGC ATTGCTTTAT 
GATGCTCATC GTCCTCATTT TAGCATCATT GGAATATCTG CTGGAGATAG TACTACTCAA 
GTATCACAGG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCTCAGAAT 
GGATTAGATC ATTATGTGTA TAAAATTGGG ATAGCATTTA ACACAGAGAT ATTTGGAACT 
TTTCGCCAAA CCATAGTTTT TGACTTTGGA TTGGAACCAG TGCTCATGCA AAGAGTAATG 
ATTGATGCTG CTTCTACAGA AGTACAAGCC AGGAAGATCC AAAGAAACAC CATAGGCTGG 
GAATTAATCT ATGTGAAGTG CAAATACCTC TGGGCTAGTT CTGCAGAAAC ACCAAGTGGG 
GACTCCTGGG GTGAGCTGGG TTTGGCTGTA CCTTCAGCAG TAACCACAGA AACTTTTACC 
TCCTGGACGG CGGTATATTT CAGAAATCGC TGGGTAAAGA CTGTCCACCC AGTTGTACAT 
CAATACTGTT TGATTTCAAG CACACATTCC ACCTTTCAGA TGCCACAGAA AGAAGACATT 
CTTAAACAAC GAGTTGTAGT TGTTACATTG AATACTTCAC AGTACCTTTG TCAACTGGAT 
CTTGAACCTG GGTTTTTTAC ACACATTCTT TTGGATGAAG CTGCACAGGC CATGGAGTGT 
GAAACCATTA TGCCTCTGGC ATTAGCTAAT AAAAACACAC GAATTGTTTT GGCTGGAGAC 
CATATGCAAC TCAGTCCTTT TGTCTACAGT GAGTTTGCCA GGGAGAGAAA CCTTCATGTT 
TCATTACTGG ATCGGCTCTA TGAACATTAT CCTGCAGAGT TTCCATGTCG GATCCTACTG 
TGTGAGAACT ATCGTTCCCA TGAAGCTATC ATCAATTACA CATCAGAACT TTTCTATGAG 
GGGAAGTTGA TGGCAAGTGG AAAACAACCA GCACACAAAG ATTTTTATCC ATTAACCTTC 
TTTACAGCAC GAGGAGAAGA TGTACAGGAA AAAAATAGTA CAGCCTTTTA TAATAATGCA 
GAGGTATTTG AAGTGGTGGA ACGGGTGGAA GAGTTAAGGA GAAAGTGGCC TGTAGCTTGG 
GGAAAATTAG ATGATGGAAG TATAGGTGTA GTCACTCCTT ATGCTGATCA GGTGTTCAGG 
ATCCGGGCAG AACTTCGAAA AAAGAGATTA TCTGATGTCA ATGTGGAAAG AGTGCTCAAT 
GTGCAGGGAA AGCAGTTTAG GGTTTTGTTT CTGAGTACAG TACGTACCCG GCACACTTGC 
AAACATAAAC AAACCCCAAT TAAAAGGAAA GAGCAACTGT TAGAAGATTC AACAGAGGAT 
TTGGATTATG GTTTTTTGTC CAACTACAAA CTTCTCAACA CCGCCATAAC AAGAGCACAG 
TCCTTGGTAG CTGTGGTGGG TGATCCTATT GCGCTGTGCT CTATTGGAAG ATGCAGGAAA 
TTTTGGGAGC GGTTTATTGC CTTGTGTCAT GACAACAAAA GCCTACATGG TATCACTTTT 
GAACAGATCA AAACACAGTT AGAGGCTTTG GAGCTAAAGA AAACATATGT GTTGAATCCA 
CTTGCTCCTG AATTTATCCC TCGGGCTCTA AGACAGCAAC ATTTGGGAAA TACCAGCAAA 
CAGCAGCAAT CACCACCCAA GGGAAAAGTC CACCATCATA ACCAAAATGA ACACTTCCAG 
AATGAAGGAA TTGTTCAACC CAATCCTTCA GTACTCATTG GCAATCCTAT TAGAGCATAT 
ACACCTCCTT CCCCTCTTGG ACCTCACCCA AATATGGGAA AGTCTCCTAG TCCTGTTCAA 
AGGATAGATC CGCACACTGG GACTAGTATT CTTTATGTAC CTGCTGTCTA TGGAGGAAAT 
GTGGTTATGT CCGTGCCTTT ACCTGTACCA TGGACAGGGT ACCAGGGTAG GTTTGCAGTT 
GACCCTCGAA TCATTACACA TCAGGCAGCG ATGGCATATA ATATGAACCT GTTGCAGACA 
CATGGACGGG GGTCTCCTAT ACCATATGGC CTTGGACATC ATTCACCAGT TAGTATAGGG 
CAACAGCAAA TTCAGCATCA GGAAAAGGAT CAACATGAAC AAAATCGAAA TGGTAAAAGT 
GATACTAATC CTGGACCTGA AATAAATAAA ATTCGTACTC CAGAGAAAAA GCCTCTAGAA 
TCAAAACAGA ATGATTTAGA TTCAAATCCA CAAAACCGAA GTCCAGAAAC TCGCCCTGGT 
GTTGCTTATC CCAATCCCAA ATTTCATCGC AAAGATAATC TCAACCCAAG ACACCTAAAC 
CTTCATCTTA CGGCTCCCCA CACACAATAT GCAGTCCCTC ATCGACATTA TCAGCACTTA 
TCCCAGCTAC CAAGACAACC ATTTCCAGTT CAACAGCCCT CAAGTTTAAA TCAGCAGCAA 
AATCATTTCC CTGAGCAACT GACTCAAATA ACACCTCAAC CAAACCAAGC AGTCCAGCAG 
CATAACCACT TGAATCAACA AACTCAGCAG CAACCCTCTC AACTTTCTCC TACATATCAA 
ATAGGTCCCA GTCATTCCTT CTTCAATAAT GCAGTCTCTC ATCGACCACA TTCTCCTGCT 
GTAGATGCAG TAGTATCTGA ACAGCACCCC CCTTCGATGC TGCAAGAAGC TAACTCTCTG 
AGATCCATTG CACAACACAA CACGATGATA CCCCCCACTC ACTTGAATAA TTTTATTGAA 
GAAAGTCCTC CAGGATTACC ATTAGGAGAA GCTTTAGAAC GTATGCATGG GAATGTGGCC 
TTAGAAACAT TAAGGCAACA ACAAGCAAGA ATACAGCAGT GGAGTGAGCA TAATGCCTAT 
CTCAGTCAAA GCAGTCTTCC ATATCCACAC TATCACCATC CTCACCTGCA GCATCTTCCC 
CAGCAACCTG CAGGATTACA TCAACAGCCA GTTAGAGCAA ACTGGAAACT TGCCAGTAAT 
GCAGAAGATG AAGCAGAAGC AACACATTCA AGGTTTCAAG ACTTACTCAG AGAGCTGGCT 
CATCATGATC AAAGTGAAAC ACGGGAGCTA ACTGAAATGC CACCACCTCA GTCAAGACTT 
TTGCAATATA GACAAATTCA AACTAGAAGC CCACCAGCAA ACCCTTCTTC TTCATCTAAT 
TCAAATCATA GTGGCCAATT TTCTAACTTC AACGACAACA TTAGAGACAT TGAAATGGCC 
AGCAATCCAG CATTTCAGCA GCATCTGCCA CCCCAAATAT ATAATCCCTC ATTTTCAATA 
CCATCTGAAA ACCTTACCCC TCCTCCTTTG AAATACCTGC CACCAGATGG ATCCTGGACC 
TTTGCTAATT TACATCAGAA TCACTTAATG GGACAGGGCT TTCACTATGG CCTACCACCA 
TTACCCCACA GACCACAACA GAACTCTTTT ATACAAATTC AGAATCAACA TGTGGTTGGT 
CAGGAGCCAT TTCACCCACT GTCATCTCGT GTGGTATCTG CTTCTTCGCT CCACAGCTTA 
GAAGAGTATG AACCACGAGG ACCAGGCAGG CCATTGTACC AAAGAAGAAT TTCATCTAGT 
TCAGTACCGC CTTGTTCTGA AGAGTTAAAC ACTCCTCAAG ATAGTCATGC TCAGGGTAAA 
GAGCATCAGG ACCATAATAA TCAATCTTCT TTCAACTATT CATCACCGGA GTTGTGGGTA 
AATACCACCT CATCTGCTCC ACATCAGAAC ATTCCATGCA ATGGATCCAG CAGAACAGCC 
CCGCCCAGAG AATTGTTAGC CCCATCAAAG CCCTTGAAAT CCTCTGAGGA TAACCTGAAA 
TCTGAAAACC TTCAGCTGTC CAATTCCTTC AACTACAGCA TGTTGCAGCA TCTAGGCCAG 
TTCCCACCCC TAATGCCCAA CAAGCAGATT GTGGAATCAA ACGGCAACAG CCAGCCAAGT 
TCTGGGGGGA ACAAGCCTAT TATGTCCTAC GCAAGTGCTC TGCGGGCACC TCCAAAGCCC 
AAACCTCCAC CTGAACAGAC AAAAAAGAAG AGTGACCCTT TGTCTCTGTT TCAGGAACTT 
AGTTTAGGTA GCTCATCAGG TAGCAATGGC TTTTATTCCT ATTTTAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HELZ cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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