Catalog Products » Protein Isolation and Purification » Protein Purification Resins » Protein A, G and L Resins

Overview

Protein A, G and L Resins

GenScript offers recombinant protein A with non-essential domains removed, recombinant protein G with albumin binding site deleted as well as codon optimized protein L. By pre-coupling these genetically modified proteins to 4% highly cross-linked agarose, GenScrtipt developed excellent antibody affinity chromatography matrix - Protein A, G and L Resins for batch/gravity purification of polyclonal and monoclonal antibody from cell culture supernatants, serum, and ascites at both laboratory and larger scale. With enhanced binding capacity, these resins enable you to get highly purified antibodies with minimal contaminations, resulting in cleaner preparations and greater yields.

Highlights

High binding capacity with ultra-low non-specific binding

High binding capacity with ultra-low non-specific binding

Fast and simple procedures for batch or gravity-flow operation

Fast and simple procedures for batch or gravity-flow operation

Compatible with a variety of sample formats and buffer additives

Compatible with a variety of sample formats and buffer additives

Increased cost-effectiveness!Resins can be easily recycled

Increased cost-effectiveness!Resins can be easily recycled

Protein Purification

Product List

Protein A, protein G and protein L are surface proteins originally found in cell wall of bacteria Staphylococcus aureus, Group G Streptococci and Peptostreptococcus magnus, respectively. Protein A and protein G bind with high affinity and specificity to Fc region of immunoglobulins from many of mammalian species, most notably IgGs. Unlike protein A and G, Protein L binds immunoglobulins through kappa light chain. These proteins have been usually used to purify, immobilize or detect immunoglobulins.

Protein A, G and L Resins

Cat. No. Product Name Quantity Price Order
L00209
 Protein G Resin
-
 
L00210
 Protein A Resin
$107.80 5 ml Resin (Total 10 ml)
 
L00239
 Protein L Resin
$420.00 100 ml
 
L00433
 Monofinity A Resin
$1680.00
 
L00464
 Protein A Resin FF
$401.70 5 ml Resin (Total 10 ml)
 
L00664
 Protein G Resin FF
$428.48
 
L00680
 Protein A Resin FF Prepacked Column
$251.00
 
L00681
 Protein G Resin FF Prepacked Column
$411.00
 

Table 1. Properties of the recombinant Protein A, G and L on Resins

Protein A Protein G Protein L
Source Staphylococcus aureus Streptococcus Peptostreptoccus magnus
Molecular weight 34 kDa 22 kDa 42 kDa
Numbers of IgG(Ig) binding sites 5 3 5
IgG Binding capacity >20 mg human IgG/ml settled resin >20 mg sheep IgG per ml settled resin >15 mg rabbit Ig per ml settled resin

Selection Guide

Selection Guide of Protein A, G and L Resins

Monofinity A Protein A Resin Ultra Protein A Resin Protein A Resin FF Protein G Resin Protein L Resin
Matrix Highly crosslinked 4% beaded agarose Agarose, 4% Agarose, 4% crosslinked Highly crosslinked 4% beaded agarose Agarose, 4% crosslinked Agarose, 4% crosslinked
Average bead size ~ 90 um ~ 90 um ~ 90 um ~ 90 um ~ 90 um
Ligand Alkali-Tolerant Protein A Recombinant Streptococcal protein A expressed in
E. coli		 Recombinant Streptococcal protein A expressed in
E. coli		 Recombinant Streptococcal protein A expressed in
E. coli		 Recombinant Streptococcal Protein G lacking the albumin-binding produced in
E. coli		 Highly purified protein L
Binding Capacity > 30 mg hIgG/ml resin > 20 mg human IgG/ml resin 30 - 40 mg hIgG /ml settled resin > 40 mg rabbit IgG/ml settled resin >20 mg sheep IgG/ml settled resin >15 mg rabbit Ig/ml settled resin
Chemical Stability 0.5 M NaOH for CIP
Stable with all commonly used reagents 		Stable with all commonly used reagents Stable with all commonly used reagents Stable with all commonly used reagents Stable with all commonly used reagents Stable with all commonly used reagents
Working pH 3-12 3-9 3-9 3-9 3-9 2 to 10
Clean in place 0.1-0.5 M NaOH 6 M guanidine hydrochloride
0.1% Triton X-100
Alternatively, wash the column with 70% ethanol 		6 M guanidine hydrochloride
0.1% Triton X-100
Alternatively, wash the column with 70% ethanol 		6 M guanidine hydrochloride
0.1% Triton X-100
Alternatively, wash the column with 70% ethanol 		0.1% Triton X-100
Alternatively, wash the column with 70% ethanol 		Washing of the packed column with 70% ethanol
15 mM NaOH
Linear flow velocity 50-300 cm/h 50-300 cm/h 50-300 cm/h 50-300 cm/h 50-300 cm/h 50-300 cm/h
Storage 20% ethanol, 2 - 8°C 20% ethanol, 2 - 8°C 1X PBS containing 20% ethanol, 2 - 8°C 1X PBS containing 20% ethanol, 2 - 8°C 1X PBS containing 20% ethanol, 2 - 8°C 1X PBS containing 20% ethanol, 2 - 8°C

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