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FGF-21, Mouse

Recombinant Mouse Fibroblast Growth Factor-21(FGF-21) produced in E.coli is a single non-glycosylated polypeptide chain containing 182 amino acids. A fully biologically active molecule, rmFGF-21 has a molecular mass of 19.9 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Z03290
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Product Introduction
Species Mouse
Protein Construction
FGF-21 (Ala29-Ser210)
Accession # Q9JJN1
Purity > 97% as analyzed by SDS-PAGE
> 97% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.5 μg/ml,measured by a cell proliferation assay using NIH-3T3 cells in the presence of 1.25 µg/ml mouse Klotho and 10.0 µg/ml heparin, corresponding to a specific activity of > 2.0 × 103 units/mg.
Expression System E. coli
Apparent Molecular Weight ~19.9 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Background
Target Background Fibroblast growth factor-21 (FGF21) belongs to the large FGF family which has at least 23 members. Along with FGF-19/15 and FGF-23, FGF-21 is categorized as a member of the atypical FGF subfamily, as it must be complexed to the Klotho co-receptor in order to bind to the FGF receptors and activate the downstream signaling pathway. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.
Synonyms FGF21; fibroblast growth factor-21; FGFL

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.