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IL-5, Rat

Recombinant rat Interleukin-5 (rrIL-5) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 113 amino acids each. A fully biologically active molecule, rrIL-5 has a molecular mass of 26.0 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
$90.00
Z03093-10

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Product Introduction
Species Rat
Protein Construction
IL-5 (Met20-Val132)
Accession # Q08125
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.4 ng/ml, measured by a cell proliferation assay using TF-1 Cells, corresponding to a specific activity of > 2.5× 106 units/mg.
Expression System E. coli
Apparent Molecular Weight ~26 kDa, on SDS-PAGE under non-reducing conditions.
Formulation Lyophilized after extensive dialysis against 20 mM Tris, pH 8.5.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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IL-5, Rat

ED₅₀< 0.4 ng/mL, measured by a cell proliferation assay using TF-1 Cells, corresponding to a specific activity of > 2.5× 10⁶ units/mg. »

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Background
Target Background Interleukin-5 (IL-5), produced by mast cells, T cells and eosinophils, is responsible for the activities attributed to eosinophil differentiating factor, B cell growth factor II and T cell-replacing factor (TRF). It can increase production and mobilization of eosinophils and CD34+ progenitors from the bone marrow. IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release.
Synonyms IL5; EDF; BCDFII; TRF
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.