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IL-1β, Mouse

Recombinant mouse Interleukin-1 Beta(rmIL-1β) produced in E. coli is a single non-glycosylated polypeptide chain containing 153 amino acids. A fully biologically active molecule, rmIL-1β is obtained by proprietary chromatographic techniques at GenScript, with an apparent molecular weight of 17.5kDa analyzed by reducing SDS-PAGE.
$100.00
Z02985-10

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Product Introduction
Species Mouse
Protein Construction

Expressed with an N-terminal Met.

IL-1β (Val118-Ser269)
Accession # P10749
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 10.0 pg/ml, measured by the dose-dependent stimulation of mouse D10.G4.1 helper T cells, corresponding to a specific activity of 1.0 × 108 IU/mg.
Expression System E. coli
Apparent Molecular Weight ~17.5 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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IL-1β, Mouse

Biological Activity
IL-1beta, Mouse (Cat. No. Z02985) stimulates cell proliferation of D10S cells. The ED50 for this effect is less than 2pg/mL. »

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Background
Target Background Interleukin-1 Beta (IL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.
Synonyms IL1B; IL-1; IL1-BETA; IL1F2; interleukin 1 beta; IL1beta
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.