Western blot analyses were performed to measure the effect of the Osi21 functional loss on the degradation of endocytosed rhodopsin.
The polyclonal rabbit antiarrestin2 antibody is diluted 1:30,000. (GenScript, NY)
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![Custom Polyclonal Antibody Production Services Banner Min]( "PolyExpress™ Polyclonal Antibody Services")
Highlights
Cost effective and comprehensive
pricing starts from $899 and includes antigen synthesis and purification!
Fast turnaround time
delivery of purified custom polyclonal antibodies in as little as 45 days.
Optimal immunization
Our OptimumAntigen design tool and intelligent Antigen Strategy increase specificity and affinity of antibodies.
Guaranteed results
affinity purified custom polyclonal antibody quantities and ELISA titers of 1:256,000.
Certified facility
AAALAC International accreditation and OLAW certification, demonstrating our commitment to responsible animal care and use.
Service Details
Details of PolyExpress™ Custom Polyclonal Antibody Production Services
Feature Details SC1180 SC1676 Immunization Antigen Synthesized 1 peptide 1 protein No. of Rabbits Immunized 2 Deliverables Negative Control Total IgG (optional: pre-immune serum or total serum protein delivery) Antigen Sample 2 mg peptide antigen 200 μg protein Antigen Affinity Purified pAb(s) 1 (2-6 mg total) 1 (2-10 mg antigen affinity purified total antibody) Guaranteed Items Application ELISA titer 1:256,000 Dot Blot/WB positive for target antigen binding; ELISA titer 1:256,000 Antibody Yield 1 purified pAb ≥ 2 mg 2-10 mg antigen affinity purified total antibody Timeline 45 days 10-11 weeks *GenScript provides peptide synthesis, peptide design, carrier conjugation and antibody purification for free.
**During the development of the antibody, if there is the death of the experimental animal, we will choose the adjusted immunization process for re-immunization to ensure the high quality and efficiency of the order.Case Studies
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Customer Case Study
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Experimental Data
Antibodies produced using PolyExpress™ have ELISA titers as high as polyclonal antibodies produced using a traditional protocol.
ELISA analysis of purified antibody generated by either PolyExpress™ protocol or traditional protocol.
ELISA analysis of purified antibody generated by PolyExpress™ Antibody Service.
PolyExpress™ generated an antibody with ELISA titer> 1: 256,000 which is higher than guaranteed.
Application Guidance
Assays Western Blot Sandwich ELISA IF, IHC,
Flow CytometryIP ChIP Requirements&Rating Rationale - Average affinity and specific recognition.
- High specificity and sensitivity.
- Ability to use same pAb as both capture and detection.
- High specificity and sensitivity.
- Conformational epitope recognition.
- Recognize multiple epitopes
- recovers more target
- overcomes obscured epitopes
- Conformational epitope recognition
SC1676 
SC1180 
Polyclonal Antibody Services Recommendations
For polyclonal antibodies targeting poorly defined epitopes we recommend protein antigens. Protein antigens provide the immune system the complete profile of exposed epitopes in the same conformational context the antibody will ultimately recognize the antigen such as FACS, sandwich ELISA, or receptor blocking for example.
Get in Touch
with GenScript Custom
Antibody Services
Related services
MonoExpress™ Antibody Service
High quality, purified monoclonal antibodies guaranteed to work in your application.
MonoRab™ Antibody Service
A novel rabbit mAb discovery platform that delivers antibodies of high target specificity & affinity for your research application.
Custom pAb Services
Generating the Yield You Need With The Speed You Want!
Quotation and Ordering
Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.
Fill out the online form and email to antibody@genscript.comA Technical Account Manager will reply you within 24 hours to finalize the quoteTo place order, provide credit card/PO informationProduction of your project begins immediatelyA Project Manager is in contact with you during the production process to give updates -
