Mouse Monoclonal Antibody Development Service

Highlights

Industry-Leading Antibody Delivery

• Deliver purified antibodies as fast as 11 wks
• Guaranteed deliverables with competitive price
• High success rate for challenging projects

Cutting-Edge Technology & Facility

• ImmunoPlus™: break the immune tolerance
• MonoExpress™: fast mouse immunization
• AAALAC and OLAW certificated animal facility

Various Antibody Production Technologies

• High-yield roller bottle antibody expression
• Serum-free recombinant antibody expression
• Ascites production for in-vivo antibody scale-up

Service Details

Custom Mouse mAb Development Package

Custom Mouse Monoclonal Antibody Package			TAT	Deliverables
Phase I (Optional) Immunogen Preparation	Customer-provided protein/peptide antigen GenScript generated protein/peptide: antigen synthesis and conjugation Other antigen type* ImmunoPlus™: to break the immune tolerance (Homology > 90%, optional)		Customer-provided: starts from 1 wk Peptide synthesis: starts from 2 wks	Deliver remaining antigen (peptide or protein) when the project is finished
Phase II Animal Immunization	Immunization options: Option 1: Conventional immunization protocol Option 2: MonoExpress™ fast immunization protocol		Conventional: starts from 6 wks MonoExpress™: ~2 wks	Deliver post-immune test samples for conventional immunization
Phase III Cell Fusion & Screening	One cell fusion with up to 15 plates Counter screening if required		4~6 wks (including the final boost)	Supernatants from up to 10~20 parental clones, 2 ml/clone CoA report
	Option 1: Hybridoma Cell Expression	Option 2: Recombinant Expression
Phase IV Subcloning, Expansion and Cryopreservation	Two rounds of subcloning 2 vials of each subclonal hybridoma cell line from up to 5 positive primary cell lines	One round of subcloning from up to 5 positive primary cell lines Antibody sequencing	Option 1: 6~8 wks Option 2: 3~5 wks	Option 1: 5 ml supernatant of each subclonal cell line Option 2: Antibody sequencing report
Phase V Ab Production (Optional)	Antibody production by roller bottle	Recombination antibody production with CHO cell	Option 1: ~4 wks Option 2: 1~2 wks	1-5 mg purified mAb/clone
Total TAT	Starts from 17 wks	Starts from 11 wks	-	-

*Immunogen type: protein, peptides, small molecules, DNA, mRNA, virus-like particles, cell lines and etc.

Standard Mouse mAb Development Package

Standard Mouse Monoclonal Antibody Package		TAT	Deliverables
Phase I (Optional) Immunogen Preparation*	Protein: Customer provides/GenScript synthesizes Peptide: GenScript synthesized only (Free of charge)	Customer provide: starts from 1 wk Peptide synthesis: 1~2 wks	Deliver the remaining antigen (2mg peptide) when the project is finished
Phase II Animal Immunization, Cell Fusion and Screening	Immunization Protocol Options: Option 1: Conventional; Option 2: MonoExpress™ no post-immunization test samples delivered for either option	Option 1: Conventional: 9~12 wks Option 2: MonoExpress™: 5~7 wks	20 ELISA (+) Supernatants, 2 ml/clone (Guaranteed) CoA report
Phase III Subcloning,Expansion,Cryopreservation and Antibody production	Small-scale production after subcloning 5 positive clones delivered Additional clones with extra charge	7~10 wks	5 subclonal hybridoma cell lines (2 vials/cell line) (Guaranteed) 5ml supernatant of each subclonal cell 1 clone purified mAb (0.1 mg) ELISA > 1:64,000 (Guaranteed) CoA
Total TAT	-	Starts from 13 wks

*:
a) Immunogen type: protein or peptide
b) Immunogen requirement for customer-provided protein: Purity ≥ 75%; Quantity: ≥ 1.5, Concentration ≥ 0.4mg/ml; Homology ≤ 90%; MW >10KD; no organic solvents in buffer; His/Flag tag protein

TurboMab Mouse mAb Development Package

TurboMab platform			TAT	Deliverables
Phase I Immunogen preparation (Optional)	Customer-provided protein/peptide antigen GenScript generated protein/peptide: antigen synthesis and conjugation Other antigen type* ImmunoPlus™: to break the immune tolerance (Homology > 90%, optional)		1~2	/
Phase II Animal Immunization	Immunization options: Option 1: Conventional immunization protocol Option 2: MonoExpress™ fast immunization protocol		Conventional: starts from 6 wks MonoExpress™: ~2 wks	Deliver post-immune test samples for conventional immunization
	Silver	Diamond
Phase III Positive B cell enrichment, cell fusion & screening	Plasma cell enrichment and cell fusion up to 10 plates	Plasma cells enrichment and cell fusion with up to 30 plates	1~2 (Report only) 2~3 (Report and supernatant)	Silver: Reports for up to 20 immunogen positive clones or with immunogen positive supernatant clones (2ml/clone) Diamond: Reports for up to 100 immunogen positive clones or with 20 immunogen positive supernatant clones (2ml/clone)
	Counter screening if required		/	/
Phase IV Sequencing & Antibody production	Option 1: Antibody sequencing and validation for up to 5 clones	/	3~4	Antibody sequencing reports and up to 5 purified Ab (for silver package only)
	Option 2: Antibody sequencing for variable region (NGS) + Gene synthesis & recombinant supernatant		3~5	Antibody sequencing reports & 0.5ml recombinant supernatant/clone (for silver or diamond package)
Phase V Ab Production (Optional)	Recombinant Ab production & Ab purification		2~3	Purified Abs, recombination positive rate not guaranteed
Total			Starts from 8 wks	-

*Immunogen type: protein, peptides, small molecules, DNA, mRNA, virus-like particles, cell lines and etc.

Case Studies

• IHC Application

  Goal: Generate mouse monoclonal antibodies specific to ADMA peptide

  Immunogen: ADMA peptide

  Immunization: MonoExpress™ + ImmunoPlus™ strategy

  Challenge: High consistency of positive and multiple counter-screening materials; immunized with peptide for IHC application

  Result: Successfully generated 287 ELISA positive clones and 5 IHC positive clones

  

  (A) Characterization of mouse monoclonal antibody against all methylated forms of XX using biotinylated peptides (AAAAAAAAAAAAA) in a custom AlphaLISA. NMA, non-methylated arginine.

  (B) Representative images of IHC staining for ADM-XX and total XX across a panel of tumor tissues and tonsil.

• WB Application

  Goal: Generate mouse monoclonal antibodies specific to KIF23-3

  Immunogen: peptide of KIF23-3 protein: DHKPASNMQTETVMQC

  Immunization: MonoExpress™ & Conventional strategy

  Challenge: 95% homologous to mice

  Result: Successfully generated WB positive clones with both conventional and MonoExpress™ immunization strategies. MonoExpress™ is able to generate more positive and specific clones to the target antigen compared to the conventional strategy.

  Conventional Immunization Strategy

  

  MonoExpress™ Immunization Strategy

  
• FACS Application

  Goal: Generate mouse monoclonal antibodies specific to mCQBL

  Immunogen: mCQBL protein

  Immunization: MonoExpress™ strategy

  Challenge: 100% homologous to mice

  Result: Successfully generate 8 FACS positive clones and 3 clones with strong cell binding activity

  

  

  Clone	1	2	3	4	5	6	7	8
  EC50	27.49	0.5272	0.3296	0.6381	113.2	/	/	51.42

• Anti-Idiotypic Antibody Application

  Case 1

  Goal: Generate mouse monoclonal antibodies specific to scFv

  Immunogen: scFv-Fc

  Immunization: MonoExpress™ strategy

  Challenge: High consistency of positive and counter screening

  Result: Successfully generated 336 scFv-Fc positive clones and 25 binding clones

  

  

  Case 2

  Goal: Generate mouse monoclonal antibodies that specific to MC-MMAE

  Immunogen: MC-MMAE-KLH & MC-MMAE-BSA

  Immunization: Conventional immunization strategy

  Result: Successfully generate 24 MC-MMAE positive clones and 5 validated clones

  

  

  

Related Resources

White Paper

Antibody Generation at its Best

Learn why hybridoma technology is preferred for high quality antibody generation, and see how it compares to other antibody generation methods!

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Additional Services

Anti-Idiotype Antibody Development

Guaranteed application in PK and ADA analysis

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Fast Mouse mAb Development

Delivers purified mouse mAb as fast as 11 wks

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Rabbit mAb Development

Delivers purified rabbit mAb as fast as 11 wks

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Large-Scale Antibody Production

Gram level antibody in-vivo & in-vitro production

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Antibody Sequencing

Sanger & High-throughput NGS Sequencing

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Specialized Antibody Services

scFv Generation Antibody Pairing Antibody Modifications

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Related Services

Overwhelmed by options? Using our Semi-custom Monoclonal Antibody Production Services, you can go from antigen sequence to antibody production with as little as 3 easy decisions. Searching for our monoclonal antibodies guaranteed to work in your system? Look no further than our MonoExpress™ Premium Service. GenScript also offers industry leading comprehensive guaranteed packages for monoclonal antibody development including:

• MonoRab™ Rabbit Monoclonal Antibody Generation Services as a better option for mouse mAb services.
• Anti-idiotype Antibodies that make PK or PD testing of therapeutic antibody drugs easy as they only detect the CDR of the target antibody.
• Monoclonal Antibody Scale-up for large quantities. Providing 15-50 mg/L monoclonal antibodies in 5-7 weeks.
• Professional Large-scale Production for gram quantities of antibodies.

Download our service selection guide to help decide which package best fits your needs.

Quotation and Ordering

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.