col21a1 cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	col21a1
Entrez Gene ID	102233886
Full Name	collagen type XXI alpha 1 chain
Gene Type	protein-coding
Organism	Xiphophorus maculatus(southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014475178.1	XP_014330664.1	collagen alpha-1(XXI) chain-like
XM_023327820.1	XP_023183588.1	collagen alpha-1(XXI) chain

Pathways from BioSystems
Gene Ontology

The following col21a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col21a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXi20432	XM_014475178.1 Latest version!	Xiphophorus maculatus collagen alpha-1(XXI) chain-like (LOC102233886), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXi33576	XM_023327820.1 Latest version!	Xiphophorus maculatus collagen type XXI alpha 1 chain (col21a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col21a1 ( XM_014475178.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_014330664.1 Xiphophorus maculatus collagen alpha-1(XXI) chain-like (LOC102233886), mRNA.

Price & Availability↑

CloneID	OXi20432
Clone ID Related Accession (Same CDS sequence)	XM_014475178.1
Accession Version	XM_014475178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1445702400000
Organism	Xiphophorus maculatus(Southern platyfish)
Product	collagen alpha-1(XXI) chain-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005373661.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xiphophorus maculatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTT GCAAAACGTT TTCCATGCAT TGGCTCCTAA GATGCCTGTG CTTTGTGCTT 
CTGTCTTTGA TTGGTGCAAC GCAAGATGAA AACTTGAGAA GCTGCAGGAC CGCTCCATGC 
GATCTAGTTT TTATTTTAGA TGGCTCATGG AGTGTTGTGG ATGTCAACTT TGAAATTGTA 
AAGCGATGGC TTGTCAACAT CACAACCAGC TTCAACATTG GACAGAAGTT TACTCAGGTC 
GGTGTGGTCC AGTATAGTGA TAACCCAATT TTAGAAATAC CTCTGGGGAT GTTCTCCTCA 
AACAAAGATC TCATCAAAGC AATGCAAAAG ATTGACTACA TGGGAGGGAA CACAAGGACG 
GGAACCGCCA TTAAGTTTGC CACTGACAAA TTGTTTGGAC TGTCCGAAAG GGGCCCAGCA 
GGCATTTCCA GAATTGCTGT TGTCCTCACA GATGGGAAAT CTCAAGATGA GGTCATGAAA 
GCAGCCGAGG AAGCAAGAAA AAAAGGGATA ATTCTGTTTG CAATTGGAGT TGGGCCAGAG 
ACAGAAGAGA ATGAGTTGAG GGACATTGCA AATAAACCTT CCACAACTTA TGTGTTCTCT 
GTTGAGGACT ACAAAGCAAT TACCAGGATT ATTCAGGTCA TACGTCAGAA ACTATGTGAA 
GAAACAGTTT GTCCAGCAAA AATTCCCATC AATTCCCGTG ACGAGAAGGG CTTCGATATT 
CTGTTAAAAT TAAATTTAGC TAAGAAAACT AAAAAAACAC AAGGATCATT TTATGGCACT 
AAAGCATATG AAGTCACATC TTTCGTTGAC TTGAGTGAAG CTACAAGGAG CCTTTTTCCT 
GATGGCTTCC CTCCATCATA TGTATTTGTG GCCACACTGC GGTATAAAGG ATCTGTAGCC 
GTGGAAAAGT GGGACCTGTG GAGAATACAG ACCCATGACG GAGATCCTCA GATGGCAGTG 
ACTCTAAATG GTCTGGATAA TACCGTCATG TTCACAACAA CCAGTAATAC ACCAAATGCA 
ATTCAAACTG TCATATTTTC ACAACAGACT GCAAGGAAGC TGTTTGATGA AAAGTGGCAC 
CAGCTGCGGC TACTGGTAAC CGAAGAGGAT GTTACTCTTT ATGTTGATGA CCTGCAAATG 
GAGACACAGC CTCTTGAGCC CTCTGTCGGC ATTTTCATCA ATGGGAAAAC CCAAGTTGGA 
AAATATGTAA ACAAGGAAAC AACTGTGCCT TTTGAAATCC AGAAACTTCG CATTTACTGT 
GACCCTGAGC AGAACACCAG AGAAACTGCA TGTGAAATAC CTGGAGTTTG CTCCAACAAT 
GCTGATTACA CTGAACCAAC ACAGGAACCT TGTACTTGTC CTCCTGGACC TCCCGGACCA 
CCAGGAACAG CAGGGGGGAA AGGAAGCTCG GGTATCCCTG GACAACCTGG ACCTGCTGGT 
GCTGATGGTA AGCCTGGTAT TCCTGGCCTT AGAGGACTTC CTGGGCTTCC AGGTCCACCA 
GGACTTAAGG GGGCACAAGG AGCAGATGGG TACAAAGGCG AGCAAGGGAG ACCGGGTGTT 
TTGGGTGAAC GGGGTATGCC TGGATTACCT GGACTGCCAG GGCAGCAAGG AGAGAAGGGT 
GCAATAGGTT TACCAGGAAT TGCAGGGTTG CCTGGTATAC CTGGTCAATT GGGTGCAAAA 
GGAAGCATAG GACCTCCTGG GCCACCAGGT ATTCCTGGAA ATCCTGGATT TCCTGGGAGA 
AATGGAAAAG ATGGAATTCT AGGAAGTCCT GGTCAAAAGG GTGAAGCTGG AGCCATTGGT 
ATTCATGGTT CAGATGGAAG ACAAGGCCAT CCTGGTGTTC CTGGTTTGCC AGGAAATGCA 
GGCCTGGACG GACAAAAGGG AGAAACTGGT GAACCTGGAC CAAAAGGAAT CAAAGGATCA 
GCTGGCCCAT CTGGGGAATT GGGCTTACCT GGTGAACCCG GCATGAAGGG ACCAATTGGA 
CCCAAGGGCA ACAAAGGTGA AACTGGTAAA CCTGGGATGA GAGGATCACA AGGGCTTGTG 
GGGAACACAG GGGAACCAGG ATTGCCAGGT CAACCGGGAC ACCAAGGCAT GCCAGGCCTG 
AAGGGAAGCA AGGGAGAAAG AGGAAACACG GGTGAAACAG GAGTGATGGG AATGAAAGGT 
GATCAAGGAG AACATGGACG ACCAGGAGTT CCGGGATCAC CTGGCCCAGT GGGATTCAAG 
GGGGAGAAAG GGACAGCAGG GGATCCAGGG CAGAGAGGGC CAGGAGGTCA AATGGGACGG 
CCTGGACAAC CTGGTCAGTC AGGGCCAACT GGGCCCCAAG GGGTGCGAGG GGACACAGGC 
CCCCCTGGGC CACAAGGACC CGAAGGCAGA TCTACAAGTG AAATGTCAGA CAGTCACATT 
CGGCAAATCT GCAGAGACAT TCTTAGAAGT GAGCTGCCTT TATTACTGCT AAGCAACCAG 
CAGAGTAGCT GTACAAGATG TCAAGGCCAA CCGGGGTCGC CTGGTGCACA AGGTCCAGCT 
GGTCCCCAAG GACTCAGAGG TCTTCCTGGA CTTAGTGGTT CCAGAGGACA ACCTGGTCGT 
CCAGGGCGTC CAGGGGTTAT TGGGCTGAAA GGAGAACCGG GTTACACAGG TGAGAAAGGG 
AGCCCTGGTC AAGTCATGGT TGGGGATCAG GGACCTCCGG GGCCACCAGG GCCTGTTGGT 
CCCCAGGGGT ACAGCAAACC AGGTCCTCCT GGTAAGCCTG GATTTCCTGG TCAAAATGGA 
GCAGAGGGTA AACCAGGTAA TCCCGGCGTT CCTGGCCATC CCGGGGTATG TGACCCATCC 
CTGTGCTATT CTAGTATAAT GAGAAGGAGT TCCTTCAGCA AAGGCCCAAA CTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014330664.1
CDS	1..2877
Translation	MMGCKTFSMHWLLRCLCFVLLSLIGATQDENLRSCRTAPCDLVFILDGSWSVVDVNFEIVKRWLVNITTSFNIGQKFTQVGVVQYSDNPILEIPLGMFSSNKDLIKAMQKIDYMGGNTRTGTAIKFATDKLFGLSERGPAGISRIAVVLTDGKSQDEVMKAAEEARKKGIILFAIGVGPETEENELRDIANKPSTTYVFSVEDYKAITRIIQVIRQKLCEETVCPAKIPINSRDEKGFDILLKLNLAKKTKKTQGSFYGTKAYEVTSFVDLSEATRSLFPDGFPPSYVFVATLRYKGSVAVEKWDLWRIQTHDGDPQMAVTLNGLDNTVMFTTTSNTPNAIQTVIFSQQTARKLFDEKWHQLRLLVTEEDVTLYVDDLQMETQPLEPSVGIFINGKTQVGKYVNKETTVPFEIQKLRIYCDPEQNTRETACEIPGVCSNNADYTEPTQEPCTCPPGPPGPPGTAGGKGSSGIPGQPGPAGADGKPGIPGLRGLPGLPGPPGLKGAQGADGYKGEQGRPGVLGERGMPGLPGLPGQQGEKGAIGLPGIAGLPGIPGQLGAKGSIGPPGPPGIPGNPGFPGRNGKDGILGSPGQKGEAGAIGIHGSDGRQGHPGVPGLPGNAGLDGQKGETGEPGPKGIKGSAGPSGELGLPGEPGMKGPIGPKGNKGETGKPGMRGSQGLVGNTGEPGLPGQPGHQGMPGLKGSKGERGNTGETGVMGMKGDQGEHGRPGVPGSPGPVGFKGEKGTAGDPGQRGPGGQMGRPGQPGQSGPTGPQGVRGDTGPPGPQGPEGRSTSEMSDSHIRQICRDILRSELPLLLLSNQQSSCTRCQGQPGSPGAQGPAGPQGLRGLPGLSGSRGQPGRPGRPGVIGLKGEPGYTGEKGSPGQVMVGDQGPPGPPGPVGPQGYSKPGPPGKPGFPGQNGAEGKPGNPGVPGHPGVCDPSLCYSSIMRRSSFSKGPNY

Target ORF information:

RefSeq Version	XM_014475178.1
Organism	Xiphophorus maculatus(Southern platyfish)
Definition	Xiphophorus maculatus collagen alpha-1(XXI) chain-like (LOC102233886), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014475178.1

ORF Insert Sequence:

ATGATGGGTT GCAAAACGTT TTCCATGCAT TGGCTCCTAA GATGCCTGTG CTTTGTGCTT 
CTGTCTTTGA TTGGTGCAAC GCAAGATGAA AACTTGAGAA GCTGCAGGAC CGCTCCATGC 
GATCTAGTTT TTATTTTAGA TGGCTCATGG AGTGTTGTGG ATGTCAACTT TGAAATTGTA 
AAGCGATGGC TTGTCAACAT CACAACCAGC TTCAACATTG GACAGAAGTT TACTCAGGTC 
GGTGTGGTCC AGTATAGTGA TAACCCAATT TTAGAAATAC CTCTGGGGAT GTTCTCCTCA 
AACAAAGATC TCATCAAAGC AATGCAAAAG ATTGACTACA TGGGAGGGAA CACAAGGACG 
GGAACCGCCA TTAAGTTTGC CACTGACAAA TTGTTTGGAC TGTCCGAAAG GGGCCCAGCA 
GGCATTTCCA GAATTGCTGT TGTCCTCACA GATGGGAAAT CTCAAGATGA GGTCATGAAA 
GCAGCCGAGG AAGCAAGAAA AAAAGGGATA ATTCTGTTTG CAATTGGAGT TGGGCCAGAG 
ACAGAAGAGA ATGAGTTGAG GGACATTGCA AATAAACCTT CCACAACTTA TGTGTTCTCT 
GTTGAGGACT ACAAAGCAAT TACCAGGATT ATTCAGGTCA TACGTCAGAA ACTATGTGAA 
GAAACAGTTT GTCCAGCAAA AATTCCCATC AATTCCCGTG ACGAGAAGGG CTTCGATATT 
CTGTTAAAAT TAAATTTAGC TAAGAAAACT AAAAAAACAC AAGGATCATT TTATGGCACT 
AAAGCATATG AAGTCACATC TTTCGTTGAC TTGAGTGAAG CTACAAGGAG CCTTTTTCCT 
GATGGCTTCC CTCCATCATA TGTATTTGTG GCCACACTGC GGTATAAAGG ATCTGTAGCC 
GTGGAAAAGT GGGACCTGTG GAGAATACAG ACCCATGACG GAGATCCTCA GATGGCAGTG 
ACTCTAAATG GTCTGGATAA TACCGTCATG TTCACAACAA CCAGTAATAC ACCAAATGCA 
ATTCAAACTG TCATATTTTC ACAACAGACT GCAAGGAAGC TGTTTGATGA AAAGTGGCAC 
CAGCTGCGGC TACTGGTAAC CGAAGAGGAT GTTACTCTTT ATGTTGATGA CCTGCAAATG 
GAGACACAGC CTCTTGAGCC CTCTGTCGGC ATTTTCATCA ATGGGAAAAC CCAAGTTGGA 
AAATATGTAA ACAAGGAAAC AACTGTGCCT TTTGAAATCC AGAAACTTCG CATTTACTGT 
GACCCTGAGC AGAACACCAG AGAAACTGCA TGTGAAATAC CTGGAGTTTG CTCCAACAAT 
GCTGATTACA CTGAACCAAC ACAGGAACCT TGTACTTGTC CTCCTGGACC TCCCGGACCA 
CCAGGAACAG CAGGGGGGAA AGGAAGCTCG GGTATCCCTG GACAACCTGG ACCTGCTGGT 
GCTGATGGTA AGCCTGGTAT TCCTGGCCTT AGAGGACTTC CTGGGCTTCC AGGTCCACCA 
GGACTTAAGG GGGCACAAGG AGCAGATGGG TACAAAGGCG AGCAAGGGAG ACCGGGTGTT 
TTGGGTGAAC GGGGTATGCC TGGATTACCT GGACTGCCAG GGCAGCAAGG AGAGAAGGGT 
GCAATAGGTT TACCAGGAAT TGCAGGGTTG CCTGGTATAC CTGGTCAATT GGGTGCAAAA 
GGAAGCATAG GACCTCCTGG GCCACCAGGT ATTCCTGGAA ATCCTGGATT TCCTGGGAGA 
AATGGAAAAG ATGGAATTCT AGGAAGTCCT GGTCAAAAGG GTGAAGCTGG AGCCATTGGT 
ATTCATGGTT CAGATGGAAG ACAAGGCCAT CCTGGTGTTC CTGGTTTGCC AGGAAATGCA 
GGCCTGGACG GACAAAAGGG AGAAACTGGT GAACCTGGAC CAAAAGGAAT CAAAGGATCA 
GCTGGCCCAT CTGGGGAATT GGGCTTACCT GGTGAACCCG GCATGAAGGG ACCAATTGGA 
CCCAAGGGCA ACAAAGGTGA AACTGGTAAA CCTGGGATGA GAGGATCACA AGGGCTTGTG 
GGGAACACAG GGGAACCAGG ATTGCCAGGT CAACCGGGAC ACCAAGGCAT GCCAGGCCTG 
AAGGGAAGCA AGGGAGAAAG AGGAAACACG GGTGAAACAG GAGTGATGGG AATGAAAGGT 
GATCAAGGAG AACATGGACG ACCAGGAGTT CCGGGATCAC CTGGCCCAGT GGGATTCAAG 
GGGGAGAAAG GGACAGCAGG GGATCCAGGG CAGAGAGGGC CAGGAGGTCA AATGGGACGG 
CCTGGACAAC CTGGTCAGTC AGGGCCAACT GGGCCCCAAG GGGTGCGAGG GGACACAGGC 
CCCCCTGGGC CACAAGGACC CGAAGGCAGA TCTACAAGTG AAATGTCAGA CAGTCACATT 
CGGCAAATCT GCAGAGACAT TCTTAGAAGT GAGCTGCCTT TATTACTGCT AAGCAACCAG 
CAGAGTAGCT GTACAAGATG TCAAGGCCAA CCGGGGTCGC CTGGTGCACA AGGTCCAGCT 
GGTCCCCAAG GACTCAGAGG TCTTCCTGGA CTTAGTGGTT CCAGAGGACA ACCTGGTCGT 
CCAGGGCGTC CAGGGGTTAT TGGGCTGAAA GGAGAACCGG GTTACACAGG TGAGAAAGGG 
AGCCCTGGTC AAGTCATGGT TGGGGATCAG GGACCTCCGG GGCCACCAGG GCCTGTTGGT 
CCCCAGGGGT ACAGCAAACC AGGTCCTCCT GGTAAGCCTG GATTTCCTGG TCAAAATGGA 
GCAGAGGGTA AACCAGGTAA TCCCGGCGTT CCTGGCCATC CCGGGGTATG TGACCCATCC 
CTGTGCTATT CTAGTATAAT GAGAAGGAGT TCCTTCAGCA AAGGCCCAAA CTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col21a1 ( XM_023327820.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023183588.1 Xiphophorus maculatus collagen type XXI alpha 1 chain (col21a1), mRNA.

Price & Availability↑

CloneID	OXi33576
Clone ID Related Accession (Same CDS sequence)	XM_023327820.1
Accession Version	XM_023327820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	collagen alpha-1(XXI) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036464.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCCGGTCT TCAGGGATAT GCTGATGTTT GTGGCAATCG CTTCCATCTC ATCATCAGTC 
CATTGTCAAA AATTGCCAAG TTTTGATCTG CTGGAGGGGT TCCATGTTCC TGAGTCGAAA 
GGAGTGAAGA GAGTGGATGG GTCTGAAGCT GAGGCAGTGG CCTACCGCAT CAACCCCTCC 
ATTCATTTAC AGAAGACATT GAGTGATGTG TATCCAGATG GACTTCCCAC TGAATACTCC 
ATTATCACAA CATTTAAAGT GAACAATGAC ACTGCTCAGA CGTCCTGGAA CCTTTGGCAA 
GTCAGTGACC CAAAAGGACG AGAACAAGTT GGCCTGCGTT TCCAGCCTGA TGGGCGGTCG 
CTGGACTTTT TTTACACTGG TCCACAAGGG ACACAGATGG TGAGGACTTT CCAAGGTTTG 
CAAAAATTGT TTAACAGAGA ATGGCACAAG TTGGCTCTGA GCGTAAAGGG CAGCCAAGTA 
AGGTTGCTGA TTGACTGCAA GGAGATCAGA GTGGAGAGCA TCGATGAGCC GAGGCCAGTT 
ATCCGACAAG GCTACACATC CATCGTGAAA CGAGCTGCAA GAGACCGCTC CGCTTCTGTG 
GATCTCCAGC AGATGGAAGT GTCATGTGAC CCAGAGCAGG CTTATTCAGA GAGCTGCTGT 
GAGATCTCCG GTGTGTGCGG GGGATATGCA GAGATCGGCC TCACAGCTGG AAGAGCAACT 
TGTAAATGCA TGCATGGACA ACCAGGACTT CAGGGCTCTC CAGGGCCAAA GGGTCACAGA 
GGGCTTCCAG GAAAACCTGG AGACCAAGGA AGACTGGGCA ACTGGGGAAT CCGTGGAAAC 
ATAGGGGACT ACGGAAACAT TGGTGAAACG GGAGCAAAGG GCCAGCAAGG AATCAAAGGA 
GAAAAAGGAA TGAGAGGACC CTGGGGACAA AGGGGAGAGA GAGGTCTGCA AGGGCTGAAA 
GGACTAAAAG GAGCTGCGGG CTTTAAGGGA ATTCGTGGGC TTCCTGGAGA CATTGGAGAG 
GCTGGGAAAC CAGGAGAAGT GGGTACTAAA GGGCTTCAAG GATATCCAGG GATTCCAGGG 
TTTGAAGGAG TGAAGGGCCA AAAAGGGAAT TCTGGGGCTA CGGGGCGGCC CGGACCCAGA 
GGAAAGCAGG GTATTGTAGG GGATCCTGGA GAAAGAGGAG CTCCTGGAGA GAAAGGAGGG 
CCCGGTATCC AAGGACCTGT AGGCAGAGCT GGGACTGTTG GATCAAAAGG TATTACTGGG 
GATCCGGGTT TACCTGGCAG AGATGGTGAT CCAGGAATAG AGGCGTATCA AGGACCCCAG 
GGTCTCAGTG GAAAACCTGG GCATAGTGGT GGAAAGGGAG AAAAGGGCAC CCTGGGGCCA 
GCAGGAGAAA TGGGTCCCCA AGGCCAAACT GGGCTGAGAG GTTACAAAGG TTCTGCAGGA 
AAGCCAGGAA GACCTGGATT TATTGGTCCG CCTGGACCTA CTGGTCATGT GGGTGTACCT 
GGAAAACCAG GAATCAAGGG TGACACAGGA ATTCAAGGAA TTAAAGGAGT TAAAGGTGCA 
GAGGGAGAAA AAGGAGTGAA AGGTCTAAAT GGAAGGGTTG GAGACAGAGG TGAGCCAGGT 
GCTCAGGGAG GCAGGGGGAA GCGTGGTCCT GTAGGCCTTC CCGGTCGTAT AGGTCCTGTT 
GGAGGAAAAG GGATTCGAGG TGAAGAAGGA CCTGAGGGCT TTCAGGGACT CCCAGGACCT 
ACGGGCCCAA CGCTTCCAGC TGAACATGTG ATTGAGGTCT GTAAGAAAGT GGTCCTGGAG 
CAGATGTCCA CCTTTGCCAA CTCAGTAAAG AGGACGTGTG CTGCAGTTTG TCCTCTCTAT 
GGGGACGTTC CCATGGGCGC CCCTGGTCCC CCCGGACAGA AAGGACCCCC AGGGCCTCCT 
GGTGACCCTG GTACTGATGG TGTTGCTGGG GAACTGGGCC TACCAGGATT CTATGGAGAA 
GGTGGTGAGC CAGGTCGACA AGGAGAATCA GGTGACAGAG GAGAGCATGG TGATAAAGGA 
TTCAAAGGTT ATGGCATTCC TGGCTTCACC GGCGATCAGG GGCTAAAGGG TCAGCGTGGA 
CGTCCCGGCA GAGCCTTTAA TGGTCAGCCT GGAAAACCAG GTGAAAGGGG ATATGCAGGC 
CGGCCGGGGC ACAGGGGCCA CCCAGGTCTC AGGGGCCCCC CAGGCATCTG TGTGACCTCT 
GGATGTGCTG AGCAGAACCC ACTCAGTGGG ACACCCCAAC CAGCACCACA GAGGCTGAGA 
AATCGCTCGT ACACCATTAA AAATCAAGAC AGCTGTATGA TGGGTTGCAA AACGTTTTCC 
ATGCATTGGC TCCTAAGATG CCTGTGCTTT GTGCTTCTGT CTTTGATTGG TGCAACGCAA 
GATGAAAACT TGAGAAGCTG CAGGACCGCT CCATGCGATC TAGTTTTTAT TTTAGATGGC 
TCATGGAGTG TTGTGGATGT CAACTTTGAA ATTGTAAAGC GATGGCTTGT CAACATCACA 
ACCAGCTTCA ACATTGGACA GAAGTTTACT CAGGTCGGTG TGGTCCAGTA TAGTGATAAC 
CCAATTTTAG AAATACCTCT GGGGATGTTC TCCTCAAACA AAGATCTCAT CAAAGCAATG 
CAAAAGATTG ACTACATGGG AGGGAACACA AGGACGGGAA CCGCCATTAA GTTTGCCACT 
GACAAATTGT TTGGACTGTC CGAAAGGGGC CCAGCAGGCA TTTCCAGAAT TGCTGTTGTC 
CTCACAGATG GGAAATCTCA AGATGAGGTC ATGAAAGCAG CCGAGGAAGC AAGAAAAAAA 
GGGATAATTC TGTTTGCAAT TGGAGTTGGG CCAGAGACAG AAGAGAATGA GTTGAGGGAC 
ATTGCAAATA AACCTTCCAC AACTTATGTG TTCTCTGTTG AGGACTACAA AGCAATTACC 
AGGATTATTC AGGTCATACG TCAGAAACTA TGTGAAGAAA CAGTTTGTCC AGCAAAAATT 
CCCATCAATT CCCGTGACGA GAAGGGCTTC GATATTCTGT TAAAATTAAA TTTAGCTAAG 
AAAACTAAAA AAACACAAGG ATCATTTTAT GGCACTAAAG CATATGAAGT CACATCTTTC 
GTTGACTTGA GTGAAGCTAC AAGGAGCCTT TTTCCTGATG GCTTCCCTCC ATCATATGTA 
TTTGTGGCCA CACTGCGGTA TAAAGGATCT GTAGCCGTGG AAAAGTGGGA CCTGTGGAGA 
ATACAGACCC ATGACGGAGA TCCTCAGATG GCAGTGACTC TAAATGGTCT GGATAATACC 
GTCATGTTCA CAACAACCAG TAATACACCA AATGCAATTC AAACTGTCAT ATTTTCACAA 
CAGACTGCAA GGAAGCTGTT TGATGAAAAG TGGCACCAGC TGCGGCTACT GGTAACCGAA 
GAGGATGTTA CTCTTTATGT TGATGACCTG CAAATGGAGA CACAGCCTCT TGAGCCCTCT 
GTCGGCATTT TCATCAATGG GAAAACCCAA GTTGGAAAAT ATGTAAACAA GGAAACAACT 
GTGCCTTTTG AAATCCAGAA ACTTCGCATT TACTGTGACC CTGAGCAGAA CACCAGAGAA 
ACTGCATGTG AAATACCTGG AGTTTGCTCC AACAATGCTG ATTACACTGA ACCAACACAG 
GAACCTTGTA CTTGTCCTCC TGGACCTCCC GGACCACCAG GAACAGCAGG GGGGAAAGGA 
AGCTCGGGTA TCCCTGGACA ACCTGGACCT GCTGGTGCTG ATGGTAAGCC TGGTATTCCT 
GGCCTTAGAG GACTTCCTGG GCTTCCAGGT CCACCAGGAC TTAAGGGGGC ACAAGGAGCA 
GATGGGTACA AAGGCGAGCA AGGGAGACCG GGTGTTTTGG GTGAACGGGG TATGCCTGGA 
TTACCTGGAC TGCCAGGGCA GCAAGGAGAG AAGGGTGCAA TAGGTTTACC AGGAATTGCA 
GGGTTGCCTG GTATACCTGG TCAATTGGGT GCAAAAGGAA GCATAGGACC TCCTGGGCCA 
CCAGGTATTC CTGGAAATCC TGGATTTCCT GGGAGAAATG GAAAAGATGG AATTCTAGGA 
AGTCCTGGTC AAAAGGGTGA AGCTGGAGCC ATTGGTATTC ATGGTTCAGA TGGAAGACAA 
GGCCATCCTG GTGTTCCTGG TTTGCCAGGA AATGCAGGCC TGGACGGACA AAAGGGAGAA 
ACTGGTGAAC CTGGACCAAA AGGAATCAAA GGATCAGCTG GCCCATCTGG GGAATTGGGC 
TTACCTGGTG AACCCGGCAT GAAGGGACCA ATTGGACCCA AGGGCAACAA AGGTGAAACT 
GGTAAACCTG GGATGAGAGG ATCACAAGGG CTTGTGGGGA ACACAGGGGA ACCAGGATTG 
CCAGGTCAAC CGGGACACCA AGGCATGCCA GGCCTGAAGG GAAGCAAGGG AGAAAGAGGA 
AACACGGGTG AAACAGGAGT GATGGGAATG AAAGGTGATC AAGGAGAACA TGGACGACCA 
GGAGTTCCGG GATCACCTGG CCCAGTGGGA TTCAAGGGGG AGAAAGGGAC AGCAGGGGAT 
CCAGGGCAGA GAGGGCCAGG AGGTCAAATG GGACGGCCTG GACAACCTGG TCAGTCAGGG 
CCAACTGGGC CCCAAGGGGT GCGAGGGGAC ACAGGCCCCC CTGGGCCACA AGGACCCGAA 
GGCAGATCTA CAAGTGAAAT GTCAGACAGT CACATTCGGC AAATCTGCAG AGACATTCTT 
AGAAGTGAGC TGCCTTTATT ACTGCTAAGC AACCAGCAGA GTAGCTGTAC AAGATGTCAA 
GGCCAACCGG GGTCGCCTGG TGCACAAGGT CCAGCTGGTC CCCAAGGACT CAGAGGTCTT 
CCTGGACTTA GTGGTTCCAG AGGACAACCT GGTCGTCCAG GGCGTCCAGG GGTTATTGGG 
CTGAAAGGAG AACCGGGTTA CACAGGTGAG AAAGGGAGCC CTGGTCAAGT CATGGTTGGG 
GATCAGGGAC CTCCGGGGCC ACCAGGGCCT GTTGGTCCCC AGGGGTACAG CAAACCAGGT 
CCTCCTGGTA AGCCTGGATT TCCTGGTCAA AATGGAGCAG AGGGTAAACC AGGTAATCCC 
GGCGTTCCTG GCCATCCCGG GGTATGTGAC CCATCCCTGT GCTATTCTAG TATAATGAGA 
AGGAGTTCCT TCAGCAAAGG CCCAAACTAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023183588.1
CDS	16..5208
Translation	MPVFRDMLMFVAIASISSSVHCQKLPSFDLLEGFHVPESKGVKRVDGSEAEAVAYRINPSIHLQKTLSDVYPDGLPTEYSIITTFKVNNDTAQTSWNLWQVSDPKGREQVGLRFQPDGRSLDFFYTGPQGTQMVRTFQGLQKLFNREWHKLALSVKGSQVRLLIDCKEIRVESIDEPRPVIRQGYTSIVKRAARDRSASVDLQQMEVSCDPEQAYSESCCEISGVCGGYAEIGLTAGRATCKCMHGQPGLQGSPGPKGHRGLPGKPGDQGRLGNWGIRGNIGDYGNIGETGAKGQQGIKGEKGMRGPWGQRGERGLQGLKGLKGAAGFKGIRGLPGDIGEAGKPGEVGTKGLQGYPGIPGFEGVKGQKGNSGATGRPGPRGKQGIVGDPGERGAPGEKGGPGIQGPVGRAGTVGSKGITGDPGLPGRDGDPGIEAYQGPQGLSGKPGHSGGKGEKGTLGPAGEMGPQGQTGLRGYKGSAGKPGRPGFIGPPGPTGHVGVPGKPGIKGDTGIQGIKGVKGAEGEKGVKGLNGRVGDRGEPGAQGGRGKRGPVGLPGRIGPVGGKGIRGEEGPEGFQGLPGPTGPTLPAEHVIEVCKKVVLEQMSTFANSVKRTCAAVCPLYGDVPMGAPGPPGQKGPPGPPGDPGTDGVAGELGLPGFYGEGGEPGRQGESGDRGEHGDKGFKGYGIPGFTGDQGLKGQRGRPGRAFNGQPGKPGERGYAGRPGHRGHPGLRGPPGICVTSGCAEQNPLSGTPQPAPQRLRNRSYTIKNQDSCMMGCKTFSMHWLLRCLCFVLLSLIGATQDENLRSCRTAPCDLVFILDGSWSVVDVNFEIVKRWLVNITTSFNIGQKFTQVGVVQYSDNPILEIPLGMFSSNKDLIKAMQKIDYMGGNTRTGTAIKFATDKLFGLSERGPAGISRIAVVLTDGKSQDEVMKAAEEARKKGIILFAIGVGPETEENELRDIANKPSTTYVFSVEDYKAITRIIQVIRQKLCEETVCPAKIPINSRDEKGFDILLKLNLAKKTKKTQGSFYGTKAYEVTSFVDLSEATRSLFPDGFPPSYVFVATLRYKGSVAVEKWDLWRIQTHDGDPQMAVTLNGLDNTVMFTTTSNTPNAIQTVIFSQQTARKLFDEKWHQLRLLVTEEDVTLYVDDLQMETQPLEPSVGIFINGKTQVGKYVNKETTVPFEIQKLRIYCDPEQNTRETACEIPGVCSNNADYTEPTQEPCTCPPGPPGPPGTAGGKGSSGIPGQPGPAGADGKPGIPGLRGLPGLPGPPGLKGAQGADGYKGEQGRPGVLGERGMPGLPGLPGQQGEKGAIGLPGIAGLPGIPGQLGAKGSIGPPGPPGIPGNPGFPGRNGKDGILGSPGQKGEAGAIGIHGSDGRQGHPGVPGLPGNAGLDGQKGETGEPGPKGIKGSAGPSGELGLPGEPGMKGPIGPKGNKGETGKPGMRGSQGLVGNTGEPGLPGQPGHQGMPGLKGSKGERGNTGETGVMGMKGDQGEHGRPGVPGSPGPVGFKGEKGTAGDPGQRGPGGQMGRPGQPGQSGPTGPQGVRGDTGPPGPQGPEGRSTSEMSDSHIRQICRDILRSELPLLLLSNQQSSCTRCQGQPGSPGAQGPAGPQGLRGLPGLSGSRGQPGRPGRPGVIGLKGEPGYTGEKGSPGQVMVGDQGPPGPPGPVGPQGYSKPGPPGKPGFPGQNGAEGKPGNPGVPGHPGVCDPSLCYSSIMRRSSFSKGPNY

Target ORF information:

RefSeq Version	XM_023327820.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus collagen type XXI alpha 1 chain (col21a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023327820.1

ORF Insert Sequence:

ATGCCGGTCT TCAGGGATAT GCTGATGTTT GTGGCAATCG CTTCCATCTC ATCATCAGTC 
CATTGTCAAA AATTGCCAAG TTTTGATCTG CTGGAGGGGT TCCATGTTCC TGAGTCGAAA 
GGAGTGAAGA GAGTGGATGG GTCTGAAGCT GAGGCAGTGG CCTACCGCAT CAACCCCTCC 
ATTCATTTAC AGAAGACATT GAGTGATGTG TATCCAGATG GACTTCCCAC TGAATACTCC 
ATTATCACAA CATTTAAAGT GAACAATGAC ACTGCTCAGA CGTCCTGGAA CCTTTGGCAA 
GTCAGTGACC CAAAAGGACG AGAACAAGTT GGCCTGCGTT TCCAGCCTGA TGGGCGGTCG 
CTGGACTTTT TTTACACTGG TCCACAAGGG ACACAGATGG TGAGGACTTT CCAAGGTTTG 
CAAAAATTGT TTAACAGAGA ATGGCACAAG TTGGCTCTGA GCGTAAAGGG CAGCCAAGTA 
AGGTTGCTGA TTGACTGCAA GGAGATCAGA GTGGAGAGCA TCGATGAGCC GAGGCCAGTT 
ATCCGACAAG GCTACACATC CATCGTGAAA CGAGCTGCAA GAGACCGCTC CGCTTCTGTG 
GATCTCCAGC AGATGGAAGT GTCATGTGAC CCAGAGCAGG CTTATTCAGA GAGCTGCTGT 
GAGATCTCCG GTGTGTGCGG GGGATATGCA GAGATCGGCC TCACAGCTGG AAGAGCAACT 
TGTAAATGCA TGCATGGACA ACCAGGACTT CAGGGCTCTC CAGGGCCAAA GGGTCACAGA 
GGGCTTCCAG GAAAACCTGG AGACCAAGGA AGACTGGGCA ACTGGGGAAT CCGTGGAAAC 
ATAGGGGACT ACGGAAACAT TGGTGAAACG GGAGCAAAGG GCCAGCAAGG AATCAAAGGA 
GAAAAAGGAA TGAGAGGACC CTGGGGACAA AGGGGAGAGA GAGGTCTGCA AGGGCTGAAA 
GGACTAAAAG GAGCTGCGGG CTTTAAGGGA ATTCGTGGGC TTCCTGGAGA CATTGGAGAG 
GCTGGGAAAC CAGGAGAAGT GGGTACTAAA GGGCTTCAAG GATATCCAGG GATTCCAGGG 
TTTGAAGGAG TGAAGGGCCA AAAAGGGAAT TCTGGGGCTA CGGGGCGGCC CGGACCCAGA 
GGAAAGCAGG GTATTGTAGG GGATCCTGGA GAAAGAGGAG CTCCTGGAGA GAAAGGAGGG 
CCCGGTATCC AAGGACCTGT AGGCAGAGCT GGGACTGTTG GATCAAAAGG TATTACTGGG 
GATCCGGGTT TACCTGGCAG AGATGGTGAT CCAGGAATAG AGGCGTATCA AGGACCCCAG 
GGTCTCAGTG GAAAACCTGG GCATAGTGGT GGAAAGGGAG AAAAGGGCAC CCTGGGGCCA 
GCAGGAGAAA TGGGTCCCCA AGGCCAAACT GGGCTGAGAG GTTACAAAGG TTCTGCAGGA 
AAGCCAGGAA GACCTGGATT TATTGGTCCG CCTGGACCTA CTGGTCATGT GGGTGTACCT 
GGAAAACCAG GAATCAAGGG TGACACAGGA ATTCAAGGAA TTAAAGGAGT TAAAGGTGCA 
GAGGGAGAAA AAGGAGTGAA AGGTCTAAAT GGAAGGGTTG GAGACAGAGG TGAGCCAGGT 
GCTCAGGGAG GCAGGGGGAA GCGTGGTCCT GTAGGCCTTC CCGGTCGTAT AGGTCCTGTT 
GGAGGAAAAG GGATTCGAGG TGAAGAAGGA CCTGAGGGCT TTCAGGGACT CCCAGGACCT 
ACGGGCCCAA CGCTTCCAGC TGAACATGTG ATTGAGGTCT GTAAGAAAGT GGTCCTGGAG 
CAGATGTCCA CCTTTGCCAA CTCAGTAAAG AGGACGTGTG CTGCAGTTTG TCCTCTCTAT 
GGGGACGTTC CCATGGGCGC CCCTGGTCCC CCCGGACAGA AAGGACCCCC AGGGCCTCCT 
GGTGACCCTG GTACTGATGG TGTTGCTGGG GAACTGGGCC TACCAGGATT CTATGGAGAA 
GGTGGTGAGC CAGGTCGACA AGGAGAATCA GGTGACAGAG GAGAGCATGG TGATAAAGGA 
TTCAAAGGTT ATGGCATTCC TGGCTTCACC GGCGATCAGG GGCTAAAGGG TCAGCGTGGA 
CGTCCCGGCA GAGCCTTTAA TGGTCAGCCT GGAAAACCAG GTGAAAGGGG ATATGCAGGC 
CGGCCGGGGC ACAGGGGCCA CCCAGGTCTC AGGGGCCCCC CAGGCATCTG TGTGACCTCT 
GGATGTGCTG AGCAGAACCC ACTCAGTGGG ACACCCCAAC CAGCACCACA GAGGCTGAGA 
AATCGCTCGT ACACCATTAA AAATCAAGAC AGCTGTATGA TGGGTTGCAA AACGTTTTCC 
ATGCATTGGC TCCTAAGATG CCTGTGCTTT GTGCTTCTGT CTTTGATTGG TGCAACGCAA 
GATGAAAACT TGAGAAGCTG CAGGACCGCT CCATGCGATC TAGTTTTTAT TTTAGATGGC 
TCATGGAGTG TTGTGGATGT CAACTTTGAA ATTGTAAAGC GATGGCTTGT CAACATCACA 
ACCAGCTTCA ACATTGGACA GAAGTTTACT CAGGTCGGTG TGGTCCAGTA TAGTGATAAC 
CCAATTTTAG AAATACCTCT GGGGATGTTC TCCTCAAACA AAGATCTCAT CAAAGCAATG 
CAAAAGATTG ACTACATGGG AGGGAACACA AGGACGGGAA CCGCCATTAA GTTTGCCACT 
GACAAATTGT TTGGACTGTC CGAAAGGGGC CCAGCAGGCA TTTCCAGAAT TGCTGTTGTC 
CTCACAGATG GGAAATCTCA AGATGAGGTC ATGAAAGCAG CCGAGGAAGC AAGAAAAAAA 
GGGATAATTC TGTTTGCAAT TGGAGTTGGG CCAGAGACAG AAGAGAATGA GTTGAGGGAC 
ATTGCAAATA AACCTTCCAC AACTTATGTG TTCTCTGTTG AGGACTACAA AGCAATTACC 
AGGATTATTC AGGTCATACG TCAGAAACTA TGTGAAGAAA CAGTTTGTCC AGCAAAAATT 
CCCATCAATT CCCGTGACGA GAAGGGCTTC GATATTCTGT TAAAATTAAA TTTAGCTAAG 
AAAACTAAAA AAACACAAGG ATCATTTTAT GGCACTAAAG CATATGAAGT CACATCTTTC 
GTTGACTTGA GTGAAGCTAC AAGGAGCCTT TTTCCTGATG GCTTCCCTCC ATCATATGTA 
TTTGTGGCCA CACTGCGGTA TAAAGGATCT GTAGCCGTGG AAAAGTGGGA CCTGTGGAGA 
ATACAGACCC ATGACGGAGA TCCTCAGATG GCAGTGACTC TAAATGGTCT GGATAATACC 
GTCATGTTCA CAACAACCAG TAATACACCA AATGCAATTC AAACTGTCAT ATTTTCACAA 
CAGACTGCAA GGAAGCTGTT TGATGAAAAG TGGCACCAGC TGCGGCTACT GGTAACCGAA 
GAGGATGTTA CTCTTTATGT TGATGACCTG CAAATGGAGA CACAGCCTCT TGAGCCCTCT 
GTCGGCATTT TCATCAATGG GAAAACCCAA GTTGGAAAAT ATGTAAACAA GGAAACAACT 
GTGCCTTTTG AAATCCAGAA ACTTCGCATT TACTGTGACC CTGAGCAGAA CACCAGAGAA 
ACTGCATGTG AAATACCTGG AGTTTGCTCC AACAATGCTG ATTACACTGA ACCAACACAG 
GAACCTTGTA CTTGTCCTCC TGGACCTCCC GGACCACCAG GAACAGCAGG GGGGAAAGGA 
AGCTCGGGTA TCCCTGGACA ACCTGGACCT GCTGGTGCTG ATGGTAAGCC TGGTATTCCT 
GGCCTTAGAG GACTTCCTGG GCTTCCAGGT CCACCAGGAC TTAAGGGGGC ACAAGGAGCA 
GATGGGTACA AAGGCGAGCA AGGGAGACCG GGTGTTTTGG GTGAACGGGG TATGCCTGGA 
TTACCTGGAC TGCCAGGGCA GCAAGGAGAG AAGGGTGCAA TAGGTTTACC AGGAATTGCA 
GGGTTGCCTG GTATACCTGG TCAATTGGGT GCAAAAGGAA GCATAGGACC TCCTGGGCCA 
CCAGGTATTC CTGGAAATCC TGGATTTCCT GGGAGAAATG GAAAAGATGG AATTCTAGGA 
AGTCCTGGTC AAAAGGGTGA AGCTGGAGCC ATTGGTATTC ATGGTTCAGA TGGAAGACAA 
GGCCATCCTG GTGTTCCTGG TTTGCCAGGA AATGCAGGCC TGGACGGACA AAAGGGAGAA 
ACTGGTGAAC CTGGACCAAA AGGAATCAAA GGATCAGCTG GCCCATCTGG GGAATTGGGC 
TTACCTGGTG AACCCGGCAT GAAGGGACCA ATTGGACCCA AGGGCAACAA AGGTGAAACT 
GGTAAACCTG GGATGAGAGG ATCACAAGGG CTTGTGGGGA ACACAGGGGA ACCAGGATTG 
CCAGGTCAAC CGGGACACCA AGGCATGCCA GGCCTGAAGG GAAGCAAGGG AGAAAGAGGA 
AACACGGGTG AAACAGGAGT GATGGGAATG AAAGGTGATC AAGGAGAACA TGGACGACCA 
GGAGTTCCGG GATCACCTGG CCCAGTGGGA TTCAAGGGGG AGAAAGGGAC AGCAGGGGAT 
CCAGGGCAGA GAGGGCCAGG AGGTCAAATG GGACGGCCTG GACAACCTGG TCAGTCAGGG 
CCAACTGGGC CCCAAGGGGT GCGAGGGGAC ACAGGCCCCC CTGGGCCACA AGGACCCGAA 
GGCAGATCTA CAAGTGAAAT GTCAGACAGT CACATTCGGC AAATCTGCAG AGACATTCTT 
AGAAGTGAGC TGCCTTTATT ACTGCTAAGC AACCAGCAGA GTAGCTGTAC AAGATGTCAA 
GGCCAACCGG GGTCGCCTGG TGCACAAGGT CCAGCTGGTC CCCAAGGACT CAGAGGTCTT 
CCTGGACTTA GTGGTTCCAG AGGACAACCT GGTCGTCCAG GGCGTCCAGG GGTTATTGGG 
CTGAAAGGAG AACCGGGTTA CACAGGTGAG AAAGGGAGCC CTGGTCAAGT CATGGTTGGG 
GATCAGGGAC CTCCGGGGCC ACCAGGGCCT GTTGGTCCCC AGGGGTACAG CAAACCAGGT 
CCTCCTGGTA AGCCTGGATT TCCTGGTCAA AATGGAGCAG AGGGTAAACC AGGTAATCCC 
GGCGTTCCTG GCCATCCCGG GGTATGTGAC CCATCCCTGT GCTATTCTAG TATAATGAGA 
AGGAGTTCCT TCAGCAAAGG CCCAAACTAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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col21a1 cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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