cenpe cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cenpe
Entrez Gene ID	102231121
Full Name	centromere protein E
Gene Type	protein-coding
Organism	Xiphophorus maculatus(southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005814215.2	XP_005814272.1	centromere-associated protein E
XM_023350241.1	XP_023206009.1	centromere-associated protein E isoform X2
XM_023350240.1	XP_023206008.1	centromere-associated protein E isoform X1

Pathways from BioSystems
Gene Ontology

The following cenpe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cenpe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXi19255	XM_005814215.2 Latest version!	Xiphophorus maculatus centromere protein E, 312kDa (cenpe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXi27176	XM_023350240.1 Latest version!	Xiphophorus maculatus centromere protein E (cenpe), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXi27177	XM_023350241.1 Latest version!	Xiphophorus maculatus centromere protein E (cenpe), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cenpe ( XM_005814215.2 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_005814272.1 Xiphophorus maculatus centromere protein E, 312kDa (cenpe), mRNA.

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CloneID	OXi19255
Clone ID Related Accession (Same CDS sequence)	XM_005814215.2
Accession Version	XM_005814215.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1445702400000
Organism	Xiphophorus maculatus(Southern platyfish)
Product	centromere-associated protein E
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005372929.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_005814215.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xiphophorus maculatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 100% of CDS bases ##RefSeq-Attributes-END##

ATGGCGGAGT CTGCTGTTAA AGTCTGTGTC CGTGTTCGTC CTGTTGCTGA GAGGGAAGAA 
AATGCAGCTT CAGAAAAGCC CGTCCAGCTG TTCTGGGAAA ATGACAAGAA ATCAATTCGT 
CAGATTGATG ATGGAAATTC CAGCAAGTGC TTCACTTTTG ACAGAGTGTT TGGTGCTGAA 
GAATCTACAA GCCAGCTGTA CCAGGACATC GCAAAGCCTC TGGTTGTGTC CACCGTTGCG 
GGTTACAATG GAACCATATT TGCCTACGGT CAGACATCTT CGGGGAAGAC GTTCACAATG 
ATGGGCAGTG ACCGTAACCG TGGAGTCATT CCTCTGGCCG TAGACGACGT CTTCCAAACT 
ATTAAAACGT TTCCAAACAA GGAATTCCTT CTCAGAGTGT CCTACATGGA AATCTACAAC 
GAAACTGTGA CGGACCTGTT GGTTGACAGC TGGAAGAGAA AACCTTTGGA AATTCGGGAA 
ACTATGAATA AAACGGTCTA CGTGGCTGAC CTGACTGAGG AGCTCGTCAC AACGTCTTCA 
CAAGCCCTCG CCTGGATCAG GAAGGGAGAA AAGAACCGTC ACTACGGAAA GACTAAAATG 
AACCAGCGGA GCAGCCGCTC GCACACCATA TTCCGTATGA ATTTAGTGGA TCTCGCTGGA 
TCTGAGAGAG CCAGTCAAAC AGGGACGGAA GGTACTCGTT TCAAAGAAGG ATGCAATATC 
AATCTCAGTC TGTTTATACT TGGACAGGTG ATCAAGAAAC TCACTGATGA AAACCAGAAA 
GGCTTTACCA GCTACAGAGA CAGCAAACTG ACCCGCATCC TGCAGAACTC CCTGGGAGGC 
AACGCTAAAA CGGTCATCAT CAGCACCATC ACGCCCGTTT CTCTGGAGGA GACCCTCGGC 
ACTCTGCAGT TTGCCAGCAC CGCAAAGAAA ATGAAGAACG ACCCTCATGT GACGGAGGTA 
TCGGATGACG GCGCGTTGCT CAAGAGGTAT CGGAATGAGA TTGTAGACCT CAAACGTCGA 
CTGAACGAGG TGTCTTCGGT CACACAGACC ACAGTGACGG AAAAAGAGGT TCTGTCTCAG 
CTGCTCCAAG AGAAGGATCA GCTCCAGAGA GAGCAAGAGG ACCGGATCAG AAACCTGACC 
AAACTGCTGG TCACCAGCTC CAACCTGGTC TCTGTTCCAA AGATGCCGAA GCGCCGGGTG 
ACGTGGGGAG GAAAGATGCT CCACGGCAGT CAGCCGGCCT GCGACGACGA CGGGATAGCT 
AACATGAACG TGACTTTTAG CAGGAAGAGG AGAGCTAATC GTTTCTGTTC CGGGGATCTA 
GTTGAAGGTA TGGAGCGACC GAAGATGGAC ATTTTTCTGA ATGGTTCGTG TGAGATGATG 
GACCTGGGCT TACTGCACTT TGTAGCTGAT GAAGACTTCT ACCCTCACTG GGAGATTCCT 
GAGGAGCCTT CAGAAGAGAT GGAGATGAGC CAGAGCTCTT TGACTGTTCG CAGCTTTGGG 
GACAGTGACC TGGCGTCTCC TGATCAAAGA TGTGCTCTCT TAGAGAAACT ATCCAACCTG 
GAGCATCAGC TCGAAACGGA GCGTCAGCAG AGGGAGGAGG CTGTGAGACA GGCCGAAACC 
CTGGACTGCC GAGTCGCAGA GCTGCAGGTC CAGTTGCAGA CTTTGCTGAA AGAAGAAGCG 
GCGGAGAAGA AGCAGTCAGA AGAGAGGCTG GCAAACCTGG AGGCGCAGCT GCGAAACGAA 
ACCCAGCAGA AGCTGGATGC CTCGTTGCAA GTGCAAGCCG CTGAACAGAA GGTTGCAGAG 
CTGGAGGAGG TCCTGCAAAC CGAGGCGCAG CAGAAGCGGG AAGCCACTTT GACCTCCACT 
TTTTTGGAGA TCAAAGTGTC TGAGCTGGAG AGGCAGCTGG AGGAGAAGAG GCAGTCTGAG 
TCTGAGCAGA TGAGGAAAGG ATTTGCAGAG AGTGTTCAGC TGTGTGAAAC CTTGGCATCA 
GAGAAGGAAG CGGTTACTGC CGAGCGAGAC CACCTGAAAC AGGAGCTGGG GATGTTTCTG 
GAGCACACGC AGAGCTTGGA GAAGGAAAAT GCTGCTCTCC ACCAAGAGCT GAAGGAGAAA 
AAGGATCTGG AAGAGTTCAA AAGTCTGGAG CTGAAGTTCA TGGAGGAACA GGAGAGTGAA 
TTACAGAAGG AAATATCGAG TCTAAAGAAG GCCATCGAAT TGTCTGAACA TCAGCGCCTT 
CAACCAGAGA TGACCCTGAC AGCCAGCAAG GAGAATCTGG AGGCTGAGCT GACTGGACCT 
GTTCTCCACA TGGCGGTGGA AAGCCATCCT GAAAAACATC AGAATGTTTC GTATGACGAC 
GTTGAGCTCA GCGCTCTAGA TGACACGCTG CCACAAAATC TGTTAGGAAG TGTGAAAGTG 
GAAAGCGGCG TCATCAGCCT GCAGGAGGAG CTGGCTCTAT GTGGAGAGGC AGAGGAGGCA 
CTAAGGGAGC AGCAGGAGGA ACAGGCTTCA CTTCAGACTC CTGGAGATCA GAGTGGAGAC 
CTGACAGGGG AGCTGCAGGA TGTTAACAAG ACGACTGACG ACCTCCAGTC AGGTCTGGCT 
GCTCAGGAGA CGGAGCTGAA GCAACAATGT GCCGATTTAA CTCAGCAGCT CGATGCACTG 
CAAGCAGAGC AGAGACAGAG CGTCCTGGAC AGGAGCCGGC TTTTAGTTTC TCTGGAAGAG 
AAAGACGCAG AGCTGAAGCA GACGATTGAA GCCCTCCAGG GTGTAGAAGC AGAGAGGGAG 
GCTCTGCTGT TGGAACGACG TGTTCAGAGC TCCGCTGAAG ATGGAAACGC AGCGACGGAC 
GTCCTCAGGT CCATTCAGGA AGAACTCAGG GAAGAAAAGC AGAGGAACTC TGACATGAAG 
ACGGCCTTTC TAGAGAAGGA GTCCGAGTTA AAGGAACATA TGCTGAGGCT AACACAGGAG 
CTCCAAGATG GCGGCAGGAG CTCCACAGAG GAGGTGGAGA GGCTGCTGAC TGCGGTCGCC 
TCTCTGACTG CAGAGAGAGA TCAGCTCAAG CAGGACCTGC AGGAAAATGT GGAGCTGATG 
ATCGAGAACC AGGACGAGCT GAGGAGCGTC CTGGAGAAGA ACCGTGAGCA GGCCAAGCAG 
ATCAAACTTC TGAAGTCTGG AAACGTCTGC AAGCAGGATG GAGGTCCCAC TGAAACGGAC 
ACTCAGCTGG AGGAAGCACA GGGAAGTATG ACCGCTTTAA GTGAGGAGCT GAGGCAAACC 
CAGGCAGAAA AAGACGGACT GCTCTCCCAG AGCGCAGCCA CTCAGACCAC AGAGGAGCTG 
GAGGAGCTGC AAGGCAGACT GGCTGCTCTT ACCGAGGAGA GAGAGGAGCT GCTGCACGCA 
GCGGAGAGGC TGAGAGGGGA GAAGGAGGAG CTCCGAGCAC AGCTGGAGGA GAAGACCCAC 
ATGATGCAGT GCGAGATGCA GCAGCAGCAG CTCCTCTCTG AAGCGAATCC ACTGAAAGAC 
AAGCAGATCC AGCAGCTGGA GGAGCTGGAG CGTCTGAGGT CTGTTCTTCA GGAGAAGGAG 
AAGCTGATGC TTGAGAAGCA GGAGGAGCTC CAGGTGCTGA AGGAGAGCAG GGAGAGGATG 
GAGGAAGATA TGATGGAGCT GAAGAGTCAG CTGGTAGAGA TGCAGAGCAG CAGCTGCAAC 
ACACACAAGC AGTTTGCTGA GGAGCTGGAG TGTGTGAAAG CAGAGAGGGA GGTTCTGCTG 
GCCCAGAGGG ACGCTGTGAG CCAGCGGTTT GAGGAGGAGC TGGAGAAGCT GCAGAGCTCC 
CTGGATACCG TCGGTCAGGA GAGGGAGGAG CTGCAGGAGG AGCTGAGACA GAAGCAGGAA 
GCTGAGATGC AGCTGCAGAG AAGAACGGAG ATGTTGGAGG CTGAGGTCAG GGAGCTGAAC 
GCGTTTGTAC AGAATGTTAC TGAGCAGAAG AGGCAGCAGG AGCAGCAACT GCAGCAACTT 
GGACAAAAGG CTTCCTCAAC AGAAGAACTT CTGAATGCTC TGCAGTATGA CTTGCGCCAA 
CAAAGAAACG TGAACTCTGA GCTGAAGAAA GCGAGTGAAG AGGAGAAACG TTCTTTGGAG 
CAGAAGATAA CAGAATTATG TAACAACTTG GAAAGTGTTG AAGCAGAGAG AGACTTTCAT 
CTGTCAGACA AAGAGGAGAA CCTGCAGAAG CACAAAGAAG AGATGGAGAA GCTGCAGCTC 
AGCGTGACGG CTCTGAGTCA GAAGAGCGAT CTGCTGCAGG AGGAGAAGGA GCAGCTCGAG 
GCGCAGCTGC AGCACAACAT CAACATGGCG TCAGCCTACC AGGAGAAGAT CACCCAGCAG 
GAGCTGCTGA TGACGCAGAA GCAAACGGAA AAAGACGAAC AAGAAGCAAC ATTTCAGCAA 
AAATGGCAGA ACCTGCAGAA GGAGCTGCAG CTGCAGAGGG AGGGAAGAGC TCAGTCTGAA 
GCCCAGCGCG ACGCCTCCCA GCAGCTGCTC AATGAAGCAA ACGTGTCTCT GTCAGCCCTG 
AGACAGCAGC TGAACAACCT GGAGCAGAAG CCCAATGCAC TCTGGGAGAC TCTGTCACAC 
CTGCAGGACT CTGCTGATCA TCTTGATGCA ACCTTTGGAA AATTCCAACA GTTTAGAGAA 
ATGTGCACAA AGACGGCCTC TGAAACCCCG GAGAACGTTC TGAAAGTCCA GAGTTTCCTG 
AAGCTTTCTG ACCTGAAGGA CCTTCCAAAG TCCAGGCTGG CTGTCTTCGG TGACGAACAG 
CTCACGGTGC ATCCACGTGT TTATATGAAA ACATTTGAGC TGCTGGTGAA TATAGACCTG 
GCTGTTTTTG AGGAGAGACG GCTGCAGGAC TTGCTGACGT CCAGAGTTGA CGCGCCTAAG 
TTTTCCCTCA ACATCGAGGA TTTCCACTCC CGGTGGAATC AGAGGCTCTC TAAGCTTCTG 
GACAAACGTC GGCTCTACCA GCAGCAAATG GAGAGCATTT TGGAGAGGCT CTGGGCCAAC 
CTGACTTCAT ATATCGCAGT GCGACAGGCT GAAGTTCAGG AGAGGGTCAA ATTCAACGAG 
TCTATCGAGG CCGCCTCCAG CCCACCGGTC ATCAGCGAGC TGGAGCGAAT CCTCGGCCAA 
GAAGCTGAAC GGAGGCGACT TGTGGTCCAA AACGGGACGA TGCCTTTACA GGTCATCAGT 
GATGAGCTCA GTAAGATTTT GGCTGAACTA ATGCAGATGG ATAAACAGAG AGATGGTCAG 
CTTAGAGAAG AGAGGAGGAA GACGTCATCC CTGCTACAGC AGCTGGAGGG AACGCCTGCG 
ATACCAAACC TGTCCTCAGT CCAGGACTAC CAGCAGCTTC AGCAAGCAGC AGGAGAGCTT 
ACAGCTCTGC AGGAGCAGCT AGAGGAGGCG AAGGCTGAAG CCAAAAACAT GGGGTCCAAC 
CACAAACGGA CCACTCAGCT CCTGCAGACT GAACTGCTGG ACACTAAAGT TCTTGTAAAG 
GAGAAGGAGA ACACAATCCT GACCTTAAAG GTCAAACTGG AAGAGTCTGA AAAGAATGCA 
TCACTCAGTG CAGCTCAGCT GAAAAACTTC CAGAGCAAAC TCCGTGAGGA GGAGGTGAAG 
CTGGCCTCCA TCACACAGGA ACACCAGCAA GAGATGGAGA AGATGAAGAC GGTGCTGAGT 
GTGAAGGAGG AGTCTCTGAG GAAGCTGAAG CAGGATCTGA GATCACAGCA TCAGGGCGAG 
GAGTCGTTCC TCACAGGGAA GGAGCTTCAC GCTCGACTAA CCAACCCCAG AGGTACGATG 
ATCCAGAGCA GCATTCTGTT GGAGAAGACC AAGCTGGAAG AAGAGGTGAA ACAGCTCCGG 
CTGCGGATCA CTGAGCTGGA AAGCTTGGTT TCCTGCCAGC AGGAGGAGAC TACCAAGTGG 
AAGAACAGAG CAATCAGTCT TAAAGGGAAG AGCAAAGCCG AGCAGGACAG GATGACCTCC 
CCCTGTACTC CTAAGAAGAG GGCCCTGTGT CCAACCTCAG ACAGCACCTT GTTGTTCAGC 
CCCACTAAAA AAAGTTACCT TCCTCCTAAG AGAGCCCTGG ATTCTCCTCG GAAACCTCTG 
GTCAGTGCAG CCTCGCTGCT GGACTCTCCC AAAAGCAGCT TTTTCAATGC AGGTGAAGGT 
TCAGAGCTGC TGACCAGAAA CTATCCCACT CAGTTCTTTG ATAACTCCAA CCTTGGAATC 
AAACCCGATG AACCAGCTGC TGAAAAACCG GACTTCTGGA ACGATGGCAA CTGCAAGACC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005814272.1
CDS	1..6246
Translation	MAESAVKVCVRVRPVAEREENAASEKPVQLFWENDKKSIRQIDDGNSSKCFTFDRVFGAEESTSQLYQDIAKPLVVSTVAGYNGTIFAYGQTSSGKTFTMMGSDRNRGVIPLAVDDVFQTIKTFPNKEFLLRVSYMEIYNETVTDLLVDSWKRKPLEIRETMNKTVYVADLTEELVTTSSQALAWIRKGEKNRHYGKTKMNQRSSRSHTIFRMNLVDLAGSERASQTGTEGTRFKEGCNINLSLFILGQVIKKLTDENQKGFTSYRDSKLTRILQNSLGGNAKTVIISTITPVSLEETLGTLQFASTAKKMKNDPHVTEVSDDGALLKRYRNEIVDLKRRLNEVSSVTQTTVTEKEVLSQLLQEKDQLQREQEDRIRNLTKLLVTSSNLVSVPKMPKRRVTWGGKMLHGSQPACDDDGIANMNVTFSRKRRANRFCSGDLVEGMERPKMDIFLNGSCEMMDLGLLHFVADEDFYPHWEIPEEPSEEMEMSQSSLTVRSFGDSDLASPDQRCALLEKLSNLEHQLETERQQREEAVRQAETLDCRVAELQVQLQTLLKEEAAEKKQSEERLANLEAQLRNETQQKLDASLQVQAAEQKVAELEEVLQTEAQQKREATLTSTFLEIKVSELERQLEEKRQSESEQMRKGFAESVQLCETLASEKEAVTAERDHLKQELGMFLEHTQSLEKENAALHQELKEKKDLEEFKSLELKFMEEQESELQKEISSLKKAIELSEHQRLQPEMTLTASKENLEAELTGPVLHMAVESHPEKHQNVSYDDVELSALDDTLPQNLLGSVKVESGVISLQEELALCGEAEEALREQQEEQASLQTPGDQSGDLTGELQDVNKTTDDLQSGLAAQETELKQQCADLTQQLDALQAEQRQSVLDRSRLLVSLEEKDAELKQTIEALQGVEAEREALLLERRVQSSAEDGNAATDVLRSIQEELREEKQRNSDMKTAFLEKESELKEHMLRLTQELQDGGRSSTEEVERLLTAVASLTAERDQLKQDLQENVELMIENQDELRSVLEKNREQAKQIKLLKSGNVCKQDGGPTETDTQLEEAQGSMTALSEELRQTQAEKDGLLSQSAATQTTEELEELQGRLAALTEEREELLHAAERLRGEKEELRAQLEEKTHMMQCEMQQQQLLSEANPLKDKQIQQLEELERLRSVLQEKEKLMLEKQEELQVLKESRERMEEDMMELKSQLVEMQSSSCNTHKQFAEELECVKAEREVLLAQRDAVSQRFEEELEKLQSSLDTVGQEREELQEELRQKQEAEMQLQRRTEMLEAEVRELNAFVQNVTEQKRQQEQQLQQLGQKASSTEELLNALQYDLRQQRNVNSELKKASEEEKRSLEQKITELCNNLESVEAERDFHLSDKEENLQKHKEEMEKLQLSVTALSQKSDLLQEEKEQLEAQLQHNINMASAYQEKITQQELLMTQKQTEKDEQEATFQQKWQNLQKELQLQREGRAQSEAQRDASQQLLNEANVSLSALRQQLNNLEQKPNALWETLSHLQDSADHLDATFGKFQQFREMCTKTASETPENVLKVQSFLKLSDLKDLPKSRLAVFGDEQLTVHPRVYMKTFELLVNIDLAVFEERRLQDLLTSRVDAPKFSLNIEDFHSRWNQRLSKLLDKRRLYQQQMESILERLWANLTSYIAVRQAEVQERVKFNESIEAASSPPVISELERILGQEAERRRLVVQNGTMPLQVISDELSKILAELMQMDKQRDGQLREERRKTSSLLQQLEGTPAIPNLSSVQDYQQLQQAAGELTALQEQLEEAKAEAKNMGSNHKRTTQLLQTELLDTKVLVKEKENTILTLKVKLEESEKNASLSAAQLKNFQSKLREEEVKLASITQEHQQEMEKMKTVLSVKEESLRKLKQDLRSQHQGEESFLTGKELHARLTNPRGTMIQSSILLEKTKLEEEVKQLRLRITELESLVSCQQEETTKWKNRAISLKGKSKAEQDRMTSPCTPKKRALCPTSDSTLLFSPTKKSYLPPKRALDSPRKPLVSAASLLDSPKSSFFNAGEGSELLTRNYPTQFFDNSNLGIKPDEPAAEKPDFWNDGNCKTQ

Target ORF information:

RefSeq Version	XM_005814215.2
Organism	Xiphophorus maculatus(Southern platyfish)
Definition	Xiphophorus maculatus centromere protein E, 312kDa (cenpe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005814215.2

ORF Insert Sequence:

ATGGCGGAGT CTGCTGTTAA AGTCTGTGTC CGTGTTCGTC CTGTTGCTGA GAGGGAAGAA 
AATGCAGCTT CAGAAAAGCC CGTCCAGCTG TTCTGGGAAA ATGACAAGAA ATCAATTCGT 
CAGATTGATG ATGGAAATTC CAGCAAGTGC TTCACTTTTG ACAGAGTGTT TGGTGCTGAA 
GAATCTACAA GCCAGCTGTA CCAGGACATC GCAAAGCCTC TGGTTGTGTC CACCGTTGCG 
GGTTACAATG GAACCATATT TGCCTACGGT CAGACATCTT CGGGGAAGAC GTTCACAATG 
ATGGGCAGTG ACCGTAACCG TGGAGTCATT CCTCTGGCCG TAGACGACGT CTTCCAAACT 
ATTAAAACGT TTCCAAACAA GGAATTCCTT CTCAGAGTGT CCTACATGGA AATCTACAAC 
GAAACTGTGA CGGACCTGTT GGTTGACAGC TGGAAGAGAA AACCTTTGGA AATTCGGGAA 
ACTATGAATA AAACGGTCTA CGTGGCTGAC CTGACTGAGG AGCTCGTCAC AACGTCTTCA 
CAAGCCCTCG CCTGGATCAG GAAGGGAGAA AAGAACCGTC ACTACGGAAA GACTAAAATG 
AACCAGCGGA GCAGCCGCTC GCACACCATA TTCCGTATGA ATTTAGTGGA TCTCGCTGGA 
TCTGAGAGAG CCAGTCAAAC AGGGACGGAA GGTACTCGTT TCAAAGAAGG ATGCAATATC 
AATCTCAGTC TGTTTATACT TGGACAGGTG ATCAAGAAAC TCACTGATGA AAACCAGAAA 
GGCTTTACCA GCTACAGAGA CAGCAAACTG ACCCGCATCC TGCAGAACTC CCTGGGAGGC 
AACGCTAAAA CGGTCATCAT CAGCACCATC ACGCCCGTTT CTCTGGAGGA GACCCTCGGC 
ACTCTGCAGT TTGCCAGCAC CGCAAAGAAA ATGAAGAACG ACCCTCATGT GACGGAGGTA 
TCGGATGACG GCGCGTTGCT CAAGAGGTAT CGGAATGAGA TTGTAGACCT CAAACGTCGA 
CTGAACGAGG TGTCTTCGGT CACACAGACC ACAGTGACGG AAAAAGAGGT TCTGTCTCAG 
CTGCTCCAAG AGAAGGATCA GCTCCAGAGA GAGCAAGAGG ACCGGATCAG AAACCTGACC 
AAACTGCTGG TCACCAGCTC CAACCTGGTC TCTGTTCCAA AGATGCCGAA GCGCCGGGTG 
ACGTGGGGAG GAAAGATGCT CCACGGCAGT CAGCCGGCCT GCGACGACGA CGGGATAGCT 
AACATGAACG TGACTTTTAG CAGGAAGAGG AGAGCTAATC GTTTCTGTTC CGGGGATCTA 
GTTGAAGGTA TGGAGCGACC GAAGATGGAC ATTTTTCTGA ATGGTTCGTG TGAGATGATG 
GACCTGGGCT TACTGCACTT TGTAGCTGAT GAAGACTTCT ACCCTCACTG GGAGATTCCT 
GAGGAGCCTT CAGAAGAGAT GGAGATGAGC CAGAGCTCTT TGACTGTTCG CAGCTTTGGG 
GACAGTGACC TGGCGTCTCC TGATCAAAGA TGTGCTCTCT TAGAGAAACT ATCCAACCTG 
GAGCATCAGC TCGAAACGGA GCGTCAGCAG AGGGAGGAGG CTGTGAGACA GGCCGAAACC 
CTGGACTGCC GAGTCGCAGA GCTGCAGGTC CAGTTGCAGA CTTTGCTGAA AGAAGAAGCG 
GCGGAGAAGA AGCAGTCAGA AGAGAGGCTG GCAAACCTGG AGGCGCAGCT GCGAAACGAA 
ACCCAGCAGA AGCTGGATGC CTCGTTGCAA GTGCAAGCCG CTGAACAGAA GGTTGCAGAG 
CTGGAGGAGG TCCTGCAAAC CGAGGCGCAG CAGAAGCGGG AAGCCACTTT GACCTCCACT 
TTTTTGGAGA TCAAAGTGTC TGAGCTGGAG AGGCAGCTGG AGGAGAAGAG GCAGTCTGAG 
TCTGAGCAGA TGAGGAAAGG ATTTGCAGAG AGTGTTCAGC TGTGTGAAAC CTTGGCATCA 
GAGAAGGAAG CGGTTACTGC CGAGCGAGAC CACCTGAAAC AGGAGCTGGG GATGTTTCTG 
GAGCACACGC AGAGCTTGGA GAAGGAAAAT GCTGCTCTCC ACCAAGAGCT GAAGGAGAAA 
AAGGATCTGG AAGAGTTCAA AAGTCTGGAG CTGAAGTTCA TGGAGGAACA GGAGAGTGAA 
TTACAGAAGG AAATATCGAG TCTAAAGAAG GCCATCGAAT TGTCTGAACA TCAGCGCCTT 
CAACCAGAGA TGACCCTGAC AGCCAGCAAG GAGAATCTGG AGGCTGAGCT GACTGGACCT 
GTTCTCCACA TGGCGGTGGA AAGCCATCCT GAAAAACATC AGAATGTTTC GTATGACGAC 
GTTGAGCTCA GCGCTCTAGA TGACACGCTG CCACAAAATC TGTTAGGAAG TGTGAAAGTG 
GAAAGCGGCG TCATCAGCCT GCAGGAGGAG CTGGCTCTAT GTGGAGAGGC AGAGGAGGCA 
CTAAGGGAGC AGCAGGAGGA ACAGGCTTCA CTTCAGACTC CTGGAGATCA GAGTGGAGAC 
CTGACAGGGG AGCTGCAGGA TGTTAACAAG ACGACTGACG ACCTCCAGTC AGGTCTGGCT 
GCTCAGGAGA CGGAGCTGAA GCAACAATGT GCCGATTTAA CTCAGCAGCT CGATGCACTG 
CAAGCAGAGC AGAGACAGAG CGTCCTGGAC AGGAGCCGGC TTTTAGTTTC TCTGGAAGAG 
AAAGACGCAG AGCTGAAGCA GACGATTGAA GCCCTCCAGG GTGTAGAAGC AGAGAGGGAG 
GCTCTGCTGT TGGAACGACG TGTTCAGAGC TCCGCTGAAG ATGGAAACGC AGCGACGGAC 
GTCCTCAGGT CCATTCAGGA AGAACTCAGG GAAGAAAAGC AGAGGAACTC TGACATGAAG 
ACGGCCTTTC TAGAGAAGGA GTCCGAGTTA AAGGAACATA TGCTGAGGCT AACACAGGAG 
CTCCAAGATG GCGGCAGGAG CTCCACAGAG GAGGTGGAGA GGCTGCTGAC TGCGGTCGCC 
TCTCTGACTG CAGAGAGAGA TCAGCTCAAG CAGGACCTGC AGGAAAATGT GGAGCTGATG 
ATCGAGAACC AGGACGAGCT GAGGAGCGTC CTGGAGAAGA ACCGTGAGCA GGCCAAGCAG 
ATCAAACTTC TGAAGTCTGG AAACGTCTGC AAGCAGGATG GAGGTCCCAC TGAAACGGAC 
ACTCAGCTGG AGGAAGCACA GGGAAGTATG ACCGCTTTAA GTGAGGAGCT GAGGCAAACC 
CAGGCAGAAA AAGACGGACT GCTCTCCCAG AGCGCAGCCA CTCAGACCAC AGAGGAGCTG 
GAGGAGCTGC AAGGCAGACT GGCTGCTCTT ACCGAGGAGA GAGAGGAGCT GCTGCACGCA 
GCGGAGAGGC TGAGAGGGGA GAAGGAGGAG CTCCGAGCAC AGCTGGAGGA GAAGACCCAC 
ATGATGCAGT GCGAGATGCA GCAGCAGCAG CTCCTCTCTG AAGCGAATCC ACTGAAAGAC 
AAGCAGATCC AGCAGCTGGA GGAGCTGGAG CGTCTGAGGT CTGTTCTTCA GGAGAAGGAG 
AAGCTGATGC TTGAGAAGCA GGAGGAGCTC CAGGTGCTGA AGGAGAGCAG GGAGAGGATG 
GAGGAAGATA TGATGGAGCT GAAGAGTCAG CTGGTAGAGA TGCAGAGCAG CAGCTGCAAC 
ACACACAAGC AGTTTGCTGA GGAGCTGGAG TGTGTGAAAG CAGAGAGGGA GGTTCTGCTG 
GCCCAGAGGG ACGCTGTGAG CCAGCGGTTT GAGGAGGAGC TGGAGAAGCT GCAGAGCTCC 
CTGGATACCG TCGGTCAGGA GAGGGAGGAG CTGCAGGAGG AGCTGAGACA GAAGCAGGAA 
GCTGAGATGC AGCTGCAGAG AAGAACGGAG ATGTTGGAGG CTGAGGTCAG GGAGCTGAAC 
GCGTTTGTAC AGAATGTTAC TGAGCAGAAG AGGCAGCAGG AGCAGCAACT GCAGCAACTT 
GGACAAAAGG CTTCCTCAAC AGAAGAACTT CTGAATGCTC TGCAGTATGA CTTGCGCCAA 
CAAAGAAACG TGAACTCTGA GCTGAAGAAA GCGAGTGAAG AGGAGAAACG TTCTTTGGAG 
CAGAAGATAA CAGAATTATG TAACAACTTG GAAAGTGTTG AAGCAGAGAG AGACTTTCAT 
CTGTCAGACA AAGAGGAGAA CCTGCAGAAG CACAAAGAAG AGATGGAGAA GCTGCAGCTC 
AGCGTGACGG CTCTGAGTCA GAAGAGCGAT CTGCTGCAGG AGGAGAAGGA GCAGCTCGAG 
GCGCAGCTGC AGCACAACAT CAACATGGCG TCAGCCTACC AGGAGAAGAT CACCCAGCAG 
GAGCTGCTGA TGACGCAGAA GCAAACGGAA AAAGACGAAC AAGAAGCAAC ATTTCAGCAA 
AAATGGCAGA ACCTGCAGAA GGAGCTGCAG CTGCAGAGGG AGGGAAGAGC TCAGTCTGAA 
GCCCAGCGCG ACGCCTCCCA GCAGCTGCTC AATGAAGCAA ACGTGTCTCT GTCAGCCCTG 
AGACAGCAGC TGAACAACCT GGAGCAGAAG CCCAATGCAC TCTGGGAGAC TCTGTCACAC 
CTGCAGGACT CTGCTGATCA TCTTGATGCA ACCTTTGGAA AATTCCAACA GTTTAGAGAA 
ATGTGCACAA AGACGGCCTC TGAAACCCCG GAGAACGTTC TGAAAGTCCA GAGTTTCCTG 
AAGCTTTCTG ACCTGAAGGA CCTTCCAAAG TCCAGGCTGG CTGTCTTCGG TGACGAACAG 
CTCACGGTGC ATCCACGTGT TTATATGAAA ACATTTGAGC TGCTGGTGAA TATAGACCTG 
GCTGTTTTTG AGGAGAGACG GCTGCAGGAC TTGCTGACGT CCAGAGTTGA CGCGCCTAAG 
TTTTCCCTCA ACATCGAGGA TTTCCACTCC CGGTGGAATC AGAGGCTCTC TAAGCTTCTG 
GACAAACGTC GGCTCTACCA GCAGCAAATG GAGAGCATTT TGGAGAGGCT CTGGGCCAAC 
CTGACTTCAT ATATCGCAGT GCGACAGGCT GAAGTTCAGG AGAGGGTCAA ATTCAACGAG 
TCTATCGAGG CCGCCTCCAG CCCACCGGTC ATCAGCGAGC TGGAGCGAAT CCTCGGCCAA 
GAAGCTGAAC GGAGGCGACT TGTGGTCCAA AACGGGACGA TGCCTTTACA GGTCATCAGT 
GATGAGCTCA GTAAGATTTT GGCTGAACTA ATGCAGATGG ATAAACAGAG AGATGGTCAG 
CTTAGAGAAG AGAGGAGGAA GACGTCATCC CTGCTACAGC AGCTGGAGGG AACGCCTGCG 
ATACCAAACC TGTCCTCAGT CCAGGACTAC CAGCAGCTTC AGCAAGCAGC AGGAGAGCTT 
ACAGCTCTGC AGGAGCAGCT AGAGGAGGCG AAGGCTGAAG CCAAAAACAT GGGGTCCAAC 
CACAAACGGA CCACTCAGCT CCTGCAGACT GAACTGCTGG ACACTAAAGT TCTTGTAAAG 
GAGAAGGAGA ACACAATCCT GACCTTAAAG GTCAAACTGG AAGAGTCTGA AAAGAATGCA 
TCACTCAGTG CAGCTCAGCT GAAAAACTTC CAGAGCAAAC TCCGTGAGGA GGAGGTGAAG 
CTGGCCTCCA TCACACAGGA ACACCAGCAA GAGATGGAGA AGATGAAGAC GGTGCTGAGT 
GTGAAGGAGG AGTCTCTGAG GAAGCTGAAG CAGGATCTGA GATCACAGCA TCAGGGCGAG 
GAGTCGTTCC TCACAGGGAA GGAGCTTCAC GCTCGACTAA CCAACCCCAG AGGTACGATG 
ATCCAGAGCA GCATTCTGTT GGAGAAGACC AAGCTGGAAG AAGAGGTGAA ACAGCTCCGG 
CTGCGGATCA CTGAGCTGGA AAGCTTGGTT TCCTGCCAGC AGGAGGAGAC TACCAAGTGG 
AAGAACAGAG CAATCAGTCT TAAAGGGAAG AGCAAAGCCG AGCAGGACAG GATGACCTCC 
CCCTGTACTC CTAAGAAGAG GGCCCTGTGT CCAACCTCAG ACAGCACCTT GTTGTTCAGC 
CCCACTAAAA AAAGTTACCT TCCTCCTAAG AGAGCCCTGG ATTCTCCTCG GAAACCTCTG 
GTCAGTGCAG CCTCGCTGCT GGACTCTCCC AAAAGCAGCT TTTTCAATGC AGGTGAAGGT 
TCAGAGCTGC TGACCAGAAA CTATCCCACT CAGTTCTTTG ATAACTCCAA CCTTGGAATC 
AAACCCGATG AACCAGCTGC TGAAAAACCG GACTTCTGGA ACGATGGCAA CTGCAAGACC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cenpe ( XM_023350240.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023206008.1 Xiphophorus maculatus centromere protein E (cenpe), transcript variant X1, mRNA.

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CloneID	OXi27176
Clone ID Related Accession (Same CDS sequence)	XM_023350240.1
Accession Version	XM_023350240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	centromere-associated protein E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036459.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGT CTGCTGTTAA AGTCTGTGTC CGTGTTCGTC CTGTTGCTGA GAGGGAAGAA 
AATGCAGCTT CAGAAAAGCC CGTCCAGCTG TTCTGGGAAA ATGACAAGAA ATCAATTCGT 
CAGATTGATG ATGGAAATTC CAGCAAGTGC TTCACTTTTG ACAGAGTGTT TGGTGCTGAA 
GAATCTACAA GCCAGCTGTA CCAGGACATC GCAAAGCCTC TGGTTGTGTC CACCGTTGCG 
GGTTACAATG GAACCATATT TGCCTACGGT CAGACATCTT CGGGGAAGAC GTTCACAATG 
ATGGGCAGTG ACCGTAACCG TGGAGTCATT CCTCTGGCCG TAGACGACGT CTTCCAAACT 
ATTAAAACGT TTCCAAACAA GGAATTCCTT CTCAGAGTGT CCTACATGGA AATCTACAAC 
GAAACTGTGA CGGACCTGTT GGTTGACAGC TGGAAGAGAA AACCTTTGGA AATTCGGGAA 
ACTATGAATA AAACGGTCTA CGTGGCTGAC CTGACTGAGG AGCTCGTCAC AACGTCTTCA 
CAAGCCCTCG CCTGGATCAG GAAGGGAGAA AAGAACCGTC ACTACGGAAA GACTAAAATG 
AACCAGCGGA GCAGCCGCTC GCACACCATA TTCCGTATGA TCCTGGAGAG CAGAGAAAGG 
AGTGATCCAG CATCTGGGGA AAATGCTGAT GGAGCCATTA TTGTTTCCCA CTTAAATTTA 
GTGGATCTCG CTGGATCTGA GAGAGCCAGT CAAACAGGGA CGGAAGGTAC TCGTTTCAAA 
GAAGGATGCA ATATCAATCT CAGTCTGTTT ATACTTGGAC AGGTGATCAA GAAACTCACT 
GATGAAAACC AGAAAGGCTT TACCAGCTAC AGAGACAGCA AACTGACCCG CATCCTGCAG 
AACTCCCTGG GAGGCAACGC TAAAACGGTC ATCATCAGCA CCATCACGCC CGTTTCTCTG 
GAGGAGACCC TCGGCACTCT GCAGTTTGCC AGCACCGCAA AGAAAATGAA GAACGACCCT 
CATGTGACGG AGGTATCGGA TGACGGCGCG TTGCTCAAGA GGTATCGGAA TGAGATTGTA 
GACCTCAAAC GTCGACTGAA CGAGGTGTCT TCGGTCACAC AGACCACAGT GACGGAAAAA 
GAGGTTCTGT CTCAGCTGCT CCAAGAGAAG GATCAGCTCC AGAGAGAGCA AGAGGACCGG 
ATCAGAAACC TGACCAAACT GCTGGTCACC AGCTCCAACC TGGTCTCTGT TCCAAAGATG 
CCGAAGCGCC GGGTGACGTG GGGAGGAAAG ATGCTCCACG GCAGTCAGCC GGCCTGCGAC 
GACGACGGGA TAGCTAACAT GAACGTGACT TTTAGCAGGA AGAGGAGAGC TAATCGTTTC 
TGTTCCGGGG ATCTAGTTGA AGCTGATGAA GACTTCTACC CTCACTGGGA GATTCCTGAG 
GAGCCTTCAG AAGAGATGGA GATGAGCCAG AGCTCTTTGA CTGTTCGCAG CTTTGGGGAC 
AGTGACCTGG CGTCTCCTGA TCAAAGATGT GCTCTCTTAG AGAAACTATC CAACCTGGAG 
CATCAGCTCG AAACGGAGCG TCAGCAGAGG GAGGAGGCTG TGAGACAGGC CGAAACCCTG 
GACTGCCGAG TCGCAGAGCT GCAGGTCCAG TTGCAGACTT TGCTGAAAGA AGAAGCGGCG 
GAGAAGAAGC AGTCAGAAGA GAGGCTGGCA AACCTGGAGG CGCAGCTGCG AAACGAAACC 
CAGCAGAAGC TGGATGCCTC GTTGCAAGTG CAAGCCGCTG AACAGAAGGT TGCAGAGCTG 
GAGGAGGTCC TGCAAACCGA GGCGCAGCAG AAGCGGGAAG CCACTTTGAC CTCCACTTTT 
TTGGAGATCA AAGTGTCTGA GCTGGAGAGG CAGCTGGAGG AGAAGAGGCA GTCTGAGTCT 
GAGCAGATGA GGAAAGGATT TGCAGAGAGT GTTCAGCTGT GTGAAACCTT GGCATCAGAG 
AAGGAAGCGG TTACTGCCGA GCGAGACCAC CTGAAACAGG AGCTGGGGAT GTTTCTGGAG 
CACACGCAGA GCTTGGAGAA GGAAAATGCT GCTCTCCACC AAGAGCTGAA GGAGAAAAAG 
GATCTGGAAG AGTTCAAAAG TCTGGAGCTG AAGTTCATGG AGGAACAGGA GAGTGAATTA 
CAGAAGGAAA TATCGAGTCT AAAGAAGGCC ATCGAATTGT CTGAACATCA GCGCCTTCAA 
CCAGAGATGA CCCTGACAGC CAGCAAGGAG AATCTGGAGG CTGAGCTGAC TGGACCTGTT 
CTCCACATGG CGGTGGAAAG CCATCCTGAA AAACATCAGA ATGTTTCGTA TGACGACGTT 
GAGCTCAGCG CTCTAGATGA CACGCTGCCA CAAAATCTGT TAGGAAGTGT GAAAGTGGAA 
AGCGGCGTCA TCAGCCTGCA GGAGGAGCTG GCTCTATGTG GAGAGGCAGA GGAGGCACTA 
AGGGAGCAGC AGGAGGAACA GGCTTCACTT CAGACTCCTG GAGATCAGAG TGGAGACCTG 
ACAGGGGAGC TGCAGGATGT TAACAAGACG ACTGACGACC TCCAGTCAGG TCTGGCTGCT 
CAGGAGACGG AGCTGAAGCA ACAATGTGCC GATTTAACTC AGCAGCTCGA TGCACTGCAA 
GCAGAGCAGA GACAGAGCGT CCTGGACAGG AGCCGGCTTT TAGTTTCTCT GGAAGAGAAA 
GACGCAGAGC TGAAGCAGAC GATTGAAGCC CTCCAGGGTG TAGAAGCAGA GAGGGAGGCT 
CTGCTGTTGG AACGACGTGT TCAGAGCTCC GCTGAAGATG GAAACGCAGC GACGGACGTC 
CTCAGGTCCA TTCAGGAAGA ACTCAGGGAA GAAAAGCAGA GGAACTCTGA CATGAAGACG 
GCCTTTCTAG AGAAGGAGTC CGAGTTAAAG GAACATATGC TGAGGCTAAC ACAGGAGCTC 
CAAGATGGCG GCAGGAGCTC CACAGAGGAG GTGGAGAGGC TGCTGACTGC GGTCGCCTCT 
CTGACTGCAG AGAGAGATCA GCTCAAGCAG GACCTGCAGG AAAATGTGGA GCTGATGATC 
GAGAACCAGG ACGAGCTGAG GAGCGTCCTG GAGAAGAACC GTGAGCAGGC CAAGCAGATC 
AAACTTCTGA AGTCTGGAAA CGTCTGCAAG CAGGATGGAG GTCCCACTGA AACGGACACT 
CAGCTGGAGG AAGCACAGGG AAGTATGACC GCTTTAAGTG AGGAGCTGAG GCAAACCCAG 
GCAGAAAAAG ACGGACTGCT CTCCCAGAGC GCAGCCACTC AGACCACAGA GGAGCTGGAG 
GAGCTGCAAG GCAGACTGGC TGCTCTTACC GAGGAGAGAG AGGAGCTGCT GCACGCAGCG 
GAGAGGCTGA GAGGGGAGAA GGAGGAGCTC CGAGCACAGC TGGAGGAGAA GACCCACATG 
ATGCAGTGCG AGATGCAGCA GCAGCAGCTC CTCTCTGAAG CGAATCCACT GAAAGACAAG 
CAGATCCAGC AGCTGGAGGA GCTGGAGCGT CTGAGGTCTG TTCTTCAGGA GAAGGAGAAG 
CTGATGCTTG AGAAGCAGGA GGAGCTCCAG GTGCTGAAGG AGAGCAGGGA GAGGATGGAG 
GAAGATATGA TGGAGCTGAA GAGTCAGCTG GTAGAGATGC AGAGCAGCAG CTGCAACACA 
CACAAGCAGT TTGCTGAGGA GCTGGAGTGT GTGAAAGCAG AGAGGGAGGT TCTGCTGGCC 
CAGAGGGACG CTGTGAGCCA GCGGTTTGAG GAGGAGCTGG AGAAGCTGCA GAGCTCCCTG 
GATACCGTCG GTCAGGAGAG GGAGGAGCTG CAGGAGGAGC TGAGACAGAA GCAGGAAGCT 
GAGATGCAGC TGCAGAGAAG AACGGAGATG TTGGAGGCTG AGGTCAGGGA GCTGAACGCG 
TTTGTACAGA ATGTTACTGA GCAGAAGAGG CAGCAGGAGC AGCAACTGCA GCAACTTGGA 
CAAAAGGCTT CCTCAACAGA AGAACTTCTG AATGCTCTGC AGTATGACTT GCGCCAACAA 
AGAAACGTGA ACTCTGAGCT GAAGAAAGCG AGTGAAGAGG AGAAACGTTC TTTGGAGCAG 
AAGATAACAG AATTATGTAA CAACTTGGAA AGTGTTGAAG CAGAGAGAGA CTTTCATCTG 
TCAGACAAAG AGGAGAACCT GCAGAAGCAC AAAGAAGAGA TGGAGAAGCT GCAGCTCAGC 
GTGACGGCTC TGAGTCAGAA GAGCGATCTG CTGCAGGAGG AGAAGGAGCA GCTCGAGGCG 
CAGCTGCAGC ACAACATCAA CATGGCGTCA GCCTACCAGG AGAAGATCAC CCAGCAGGAG 
CTGCTGATGA CGCAGAAGCA AACGGAAAAA GACGAACAAG AAGCAACATT TCAGCAAAAA 
TGGCAGAACC TGCAGAAGGA GCTGCAGCTG CAGAGGGAGG GAAGAGCTCA GTCTGAAGCC 
CAGCGCGACG CCTCCCAGCA GCTGCTCAAT GAAGCAAACG TGTCTCTGTC AGCCCTGAGA 
CAGCAGCTGA ACAACCTGGA GCAGAAGCCC AATGCACTCT GGGAGACTCT GTCACACCTG 
CAGGACTCTG CTGATCATCT TGATGCAACC TTTGGAAAAT TCCAACAGTT TAGAGAAATG 
TGCACAAAGA CGGCCTCTGA AACCCCGGAG AACGTTCTGA AAGTCCAGAG TTTCCTGAAG 
CTTTCTGACC TGAAGGACCT TCCAAAGTCC AGGCTGGCTG TCTTCGGTGA CGTTTGTGAG 
CTGGGATTTC AAACCTGCAG CAGTTTCACA AAGACTCTTG AACAGCTCAC GGTGCATCCA 
CGTGTTTATA TGAAAACATT TGAGCTGCTG GTGAATATAG ACCTGGCTGT TTTTGAGGAG 
AGACGGCTGC AGGACTTGCT GACGTCCAGA GTTGACGCGC CTAAGTTTTC CCTCAACATC 
GAGGATTTCC ACTCCCGGTG GAATCAGAGG CTCTCTAAGC TTCTGGACAA ACGTCGGCTC 
TACCAGCAGC AAATGGAGAG CATTTTGGAG AGGCTCTGGG CCAACCTGAC TTCATATATC 
GCAGTGCGAC AGGCTGAAGT TCAGGAGAGG GTCAAATTCA ACGAGTCTAT CGAGGCCGCC 
TCCAGCCCAC CGGTCATCAG CGAGCTGGAG CGAATCCTCG GCCAAGAAGC TGAACGGAGG 
CGACTTGTGG TCCAAAACGG GACGATGCCT TTACAGGTCA TCAGTGATGA GCTCAGTAAG 
ATTTTGGCTG AACTAATGCA GATGGATAAA CAGAGAGATG GTCAGCTTAG AGAAGAGAGG 
AGGAAGACGT CATCCCTGCT ACAGCAGCTG GAGGGAACGC CTGCGATACC AAACCTGTCC 
TCAGTCCAGG ACTACCAGCA GCTTCAGCAA GCAGCAGGAG AGCTTACAGC TCTGCAGGAG 
CAGCTAGAGG AGGCGAAGGC TGAAGCCAAA AACATGGGGT CCAACCACAA ACGGACCACT 
CAGCTCCTGC AGACTGAACT GCTGGACACT AAAGTTCTTG TAAAGGAGAA GGAGAACACA 
ATCCTGACCT TAAAGGTCAA ACTGGAAGAG TCTGAAAAGA ATGCATCACT CAGTGCAGCT 
CAGCTGAAAA ACTTCCAGAG CAAACTCCGT GAGGAGGAGG TGAAGCTGGC CTCCATCACA 
CAGGAACACC AGCAAGAGAT GGAGAAGATG AAGACGGTGC TGAGTGTGAA GGAGGAGTCT 
CTGAGGAAGC TGAAGCAGGA TCTGAGATCA CAGCATCAGG GCGAGGAGTC GTTCCTCACA 
GGGAAGGAGC TTCACGCTCG ACTAACCAAC CCCAGAGGTA CGATGATCCA GAGCAGCATT 
CTGTTGGAGA AGACCAAGCT GGAAGAAGAG GTGAAACAGC TCCGGCTGCG GATCACTGAG 
CTGGAAAGCT TGGTTTCCTG CCAGCAGGAG GAGACTACCA AGTGGAAGAA CAGAGCAATC 
AGTCTTAAAG GGAAGAGCAA AGCCGAGCAG GACAGGATGA CCTCCCCCTG TACTCCTAAG 
AAGAGGGCCC TGTGTCCAAC CTCAGACAGC ACCTTGTTGT TCAGCCCCAC TAAAAAAAGT 
TACCTTCCTC CTAAGAGAGC CCTGGATTCT CCTCGGAAAC CTCTGGTCAG TGCAGCCTCG 
CTGCTGGACT CTCCCAAAAG CAGCTTTTTC AATGCAGGTG AAGGTTCAGA GCTGCTGACC 
AGAAACTATC CCACTCAGTT CTTTGATAAC TCCAACCTTG GAATCAAACC CGATGAACCA 
GCTGCTGAAA AACCGGACTT CTGGAACGAT GGCAACTGCA AGACCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023206008.1
CDS	223..6513
Translation	MAESAVKVCVRVRPVAEREENAASEKPVQLFWENDKKSIRQIDDGNSSKCFTFDRVFGAEESTSQLYQDIAKPLVVSTVAGYNGTIFAYGQTSSGKTFTMMGSDRNRGVIPLAVDDVFQTIKTFPNKEFLLRVSYMEIYNETVTDLLVDSWKRKPLEIRETMNKTVYVADLTEELVTTSSQALAWIRKGEKNRHYGKTKMNQRSSRSHTIFRMILESRERSDPASGENADGAIIVSHLNLVDLAGSERASQTGTEGTRFKEGCNINLSLFILGQVIKKLTDENQKGFTSYRDSKLTRILQNSLGGNAKTVIISTITPVSLEETLGTLQFASTAKKMKNDPHVTEVSDDGALLKRYRNEIVDLKRRLNEVSSVTQTTVTEKEVLSQLLQEKDQLQREQEDRIRNLTKLLVTSSNLVSVPKMPKRRVTWGGKMLHGSQPACDDDGIANMNVTFSRKRRANRFCSGDLVEADEDFYPHWEIPEEPSEEMEMSQSSLTVRSFGDSDLASPDQRCALLEKLSNLEHQLETERQQREEAVRQAETLDCRVAELQVQLQTLLKEEAAEKKQSEERLANLEAQLRNETQQKLDASLQVQAAEQKVAELEEVLQTEAQQKREATLTSTFLEIKVSELERQLEEKRQSESEQMRKGFAESVQLCETLASEKEAVTAERDHLKQELGMFLEHTQSLEKENAALHQELKEKKDLEEFKSLELKFMEEQESELQKEISSLKKAIELSEHQRLQPEMTLTASKENLEAELTGPVLHMAVESHPEKHQNVSYDDVELSALDDTLPQNLLGSVKVESGVISLQEELALCGEAEEALREQQEEQASLQTPGDQSGDLTGELQDVNKTTDDLQSGLAAQETELKQQCADLTQQLDALQAEQRQSVLDRSRLLVSLEEKDAELKQTIEALQGVEAEREALLLERRVQSSAEDGNAATDVLRSIQEELREEKQRNSDMKTAFLEKESELKEHMLRLTQELQDGGRSSTEEVERLLTAVASLTAERDQLKQDLQENVELMIENQDELRSVLEKNREQAKQIKLLKSGNVCKQDGGPTETDTQLEEAQGSMTALSEELRQTQAEKDGLLSQSAATQTTEELEELQGRLAALTEEREELLHAAERLRGEKEELRAQLEEKTHMMQCEMQQQQLLSEANPLKDKQIQQLEELERLRSVLQEKEKLMLEKQEELQVLKESRERMEEDMMELKSQLVEMQSSSCNTHKQFAEELECVKAEREVLLAQRDAVSQRFEEELEKLQSSLDTVGQEREELQEELRQKQEAEMQLQRRTEMLEAEVRELNAFVQNVTEQKRQQEQQLQQLGQKASSTEELLNALQYDLRQQRNVNSELKKASEEEKRSLEQKITELCNNLESVEAERDFHLSDKEENLQKHKEEMEKLQLSVTALSQKSDLLQEEKEQLEAQLQHNINMASAYQEKITQQELLMTQKQTEKDEQEATFQQKWQNLQKELQLQREGRAQSEAQRDASQQLLNEANVSLSALRQQLNNLEQKPNALWETLSHLQDSADHLDATFGKFQQFREMCTKTASETPENVLKVQSFLKLSDLKDLPKSRLAVFGDVCELGFQTCSSFTKTLEQLTVHPRVYMKTFELLVNIDLAVFEERRLQDLLTSRVDAPKFSLNIEDFHSRWNQRLSKLLDKRRLYQQQMESILERLWANLTSYIAVRQAEVQERVKFNESIEAASSPPVISELERILGQEAERRRLVVQNGTMPLQVISDELSKILAELMQMDKQRDGQLREERRKTSSLLQQLEGTPAIPNLSSVQDYQQLQQAAGELTALQEQLEEAKAEAKNMGSNHKRTTQLLQTELLDTKVLVKEKENTILTLKVKLEESEKNASLSAAQLKNFQSKLREEEVKLASITQEHQQEMEKMKTVLSVKEESLRKLKQDLRSQHQGEESFLTGKELHARLTNPRGTMIQSSILLEKTKLEEEVKQLRLRITELESLVSCQQEETTKWKNRAISLKGKSKAEQDRMTSPCTPKKRALCPTSDSTLLFSPTKKSYLPPKRALDSPRKPLVSAASLLDSPKSSFFNAGEGSELLTRNYPTQFFDNSNLGIKPDEPAAEKPDFWNDGNCKTQ

Target ORF information:

RefSeq Version	XM_023350240.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus centromere protein E (cenpe), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023350240.1

ORF Insert Sequence:

ATGGCGGAGT CTGCTGTTAA AGTCTGTGTC CGTGTTCGTC CTGTTGCTGA GAGGGAAGAA 
AATGCAGCTT CAGAAAAGCC CGTCCAGCTG TTCTGGGAAA ATGACAAGAA ATCAATTCGT 
CAGATTGATG ATGGAAATTC CAGCAAGTGC TTCACTTTTG ACAGAGTGTT TGGTGCTGAA 
GAATCTACAA GCCAGCTGTA CCAGGACATC GCAAAGCCTC TGGTTGTGTC CACCGTTGCG 
GGTTACAATG GAACCATATT TGCCTACGGT CAGACATCTT CGGGGAAGAC GTTCACAATG 
ATGGGCAGTG ACCGTAACCG TGGAGTCATT CCTCTGGCCG TAGACGACGT CTTCCAAACT 
ATTAAAACGT TTCCAAACAA GGAATTCCTT CTCAGAGTGT CCTACATGGA AATCTACAAC 
GAAACTGTGA CGGACCTGTT GGTTGACAGC TGGAAGAGAA AACCTTTGGA AATTCGGGAA 
ACTATGAATA AAACGGTCTA CGTGGCTGAC CTGACTGAGG AGCTCGTCAC AACGTCTTCA 
CAAGCCCTCG CCTGGATCAG GAAGGGAGAA AAGAACCGTC ACTACGGAAA GACTAAAATG 
AACCAGCGGA GCAGCCGCTC GCACACCATA TTCCGTATGA TCCTGGAGAG CAGAGAAAGG 
AGTGATCCAG CATCTGGGGA AAATGCTGAT GGAGCCATTA TTGTTTCCCA CTTAAATTTA 
GTGGATCTCG CTGGATCTGA GAGAGCCAGT CAAACAGGGA CGGAAGGTAC TCGTTTCAAA 
GAAGGATGCA ATATCAATCT CAGTCTGTTT ATACTTGGAC AGGTGATCAA GAAACTCACT 
GATGAAAACC AGAAAGGCTT TACCAGCTAC AGAGACAGCA AACTGACCCG CATCCTGCAG 
AACTCCCTGG GAGGCAACGC TAAAACGGTC ATCATCAGCA CCATCACGCC CGTTTCTCTG 
GAGGAGACCC TCGGCACTCT GCAGTTTGCC AGCACCGCAA AGAAAATGAA GAACGACCCT 
CATGTGACGG AGGTATCGGA TGACGGCGCG TTGCTCAAGA GGTATCGGAA TGAGATTGTA 
GACCTCAAAC GTCGACTGAA CGAGGTGTCT TCGGTCACAC AGACCACAGT GACGGAAAAA 
GAGGTTCTGT CTCAGCTGCT CCAAGAGAAG GATCAGCTCC AGAGAGAGCA AGAGGACCGG 
ATCAGAAACC TGACCAAACT GCTGGTCACC AGCTCCAACC TGGTCTCTGT TCCAAAGATG 
CCGAAGCGCC GGGTGACGTG GGGAGGAAAG ATGCTCCACG GCAGTCAGCC GGCCTGCGAC 
GACGACGGGA TAGCTAACAT GAACGTGACT TTTAGCAGGA AGAGGAGAGC TAATCGTTTC 
TGTTCCGGGG ATCTAGTTGA AGCTGATGAA GACTTCTACC CTCACTGGGA GATTCCTGAG 
GAGCCTTCAG AAGAGATGGA GATGAGCCAG AGCTCTTTGA CTGTTCGCAG CTTTGGGGAC 
AGTGACCTGG CGTCTCCTGA TCAAAGATGT GCTCTCTTAG AGAAACTATC CAACCTGGAG 
CATCAGCTCG AAACGGAGCG TCAGCAGAGG GAGGAGGCTG TGAGACAGGC CGAAACCCTG 
GACTGCCGAG TCGCAGAGCT GCAGGTCCAG TTGCAGACTT TGCTGAAAGA AGAAGCGGCG 
GAGAAGAAGC AGTCAGAAGA GAGGCTGGCA AACCTGGAGG CGCAGCTGCG AAACGAAACC 
CAGCAGAAGC TGGATGCCTC GTTGCAAGTG CAAGCCGCTG AACAGAAGGT TGCAGAGCTG 
GAGGAGGTCC TGCAAACCGA GGCGCAGCAG AAGCGGGAAG CCACTTTGAC CTCCACTTTT 
TTGGAGATCA AAGTGTCTGA GCTGGAGAGG CAGCTGGAGG AGAAGAGGCA GTCTGAGTCT 
GAGCAGATGA GGAAAGGATT TGCAGAGAGT GTTCAGCTGT GTGAAACCTT GGCATCAGAG 
AAGGAAGCGG TTACTGCCGA GCGAGACCAC CTGAAACAGG AGCTGGGGAT GTTTCTGGAG 
CACACGCAGA GCTTGGAGAA GGAAAATGCT GCTCTCCACC AAGAGCTGAA GGAGAAAAAG 
GATCTGGAAG AGTTCAAAAG TCTGGAGCTG AAGTTCATGG AGGAACAGGA GAGTGAATTA 
CAGAAGGAAA TATCGAGTCT AAAGAAGGCC ATCGAATTGT CTGAACATCA GCGCCTTCAA 
CCAGAGATGA CCCTGACAGC CAGCAAGGAG AATCTGGAGG CTGAGCTGAC TGGACCTGTT 
CTCCACATGG CGGTGGAAAG CCATCCTGAA AAACATCAGA ATGTTTCGTA TGACGACGTT 
GAGCTCAGCG CTCTAGATGA CACGCTGCCA CAAAATCTGT TAGGAAGTGT GAAAGTGGAA 
AGCGGCGTCA TCAGCCTGCA GGAGGAGCTG GCTCTATGTG GAGAGGCAGA GGAGGCACTA 
AGGGAGCAGC AGGAGGAACA GGCTTCACTT CAGACTCCTG GAGATCAGAG TGGAGACCTG 
ACAGGGGAGC TGCAGGATGT TAACAAGACG ACTGACGACC TCCAGTCAGG TCTGGCTGCT 
CAGGAGACGG AGCTGAAGCA ACAATGTGCC GATTTAACTC AGCAGCTCGA TGCACTGCAA 
GCAGAGCAGA GACAGAGCGT CCTGGACAGG AGCCGGCTTT TAGTTTCTCT GGAAGAGAAA 
GACGCAGAGC TGAAGCAGAC GATTGAAGCC CTCCAGGGTG TAGAAGCAGA GAGGGAGGCT 
CTGCTGTTGG AACGACGTGT TCAGAGCTCC GCTGAAGATG GAAACGCAGC GACGGACGTC 
CTCAGGTCCA TTCAGGAAGA ACTCAGGGAA GAAAAGCAGA GGAACTCTGA CATGAAGACG 
GCCTTTCTAG AGAAGGAGTC CGAGTTAAAG GAACATATGC TGAGGCTAAC ACAGGAGCTC 
CAAGATGGCG GCAGGAGCTC CACAGAGGAG GTGGAGAGGC TGCTGACTGC GGTCGCCTCT 
CTGACTGCAG AGAGAGATCA GCTCAAGCAG GACCTGCAGG AAAATGTGGA GCTGATGATC 
GAGAACCAGG ACGAGCTGAG GAGCGTCCTG GAGAAGAACC GTGAGCAGGC CAAGCAGATC 
AAACTTCTGA AGTCTGGAAA CGTCTGCAAG CAGGATGGAG GTCCCACTGA AACGGACACT 
CAGCTGGAGG AAGCACAGGG AAGTATGACC GCTTTAAGTG AGGAGCTGAG GCAAACCCAG 
GCAGAAAAAG ACGGACTGCT CTCCCAGAGC GCAGCCACTC AGACCACAGA GGAGCTGGAG 
GAGCTGCAAG GCAGACTGGC TGCTCTTACC GAGGAGAGAG AGGAGCTGCT GCACGCAGCG 
GAGAGGCTGA GAGGGGAGAA GGAGGAGCTC CGAGCACAGC TGGAGGAGAA GACCCACATG 
ATGCAGTGCG AGATGCAGCA GCAGCAGCTC CTCTCTGAAG CGAATCCACT GAAAGACAAG 
CAGATCCAGC AGCTGGAGGA GCTGGAGCGT CTGAGGTCTG TTCTTCAGGA GAAGGAGAAG 
CTGATGCTTG AGAAGCAGGA GGAGCTCCAG GTGCTGAAGG AGAGCAGGGA GAGGATGGAG 
GAAGATATGA TGGAGCTGAA GAGTCAGCTG GTAGAGATGC AGAGCAGCAG CTGCAACACA 
CACAAGCAGT TTGCTGAGGA GCTGGAGTGT GTGAAAGCAG AGAGGGAGGT TCTGCTGGCC 
CAGAGGGACG CTGTGAGCCA GCGGTTTGAG GAGGAGCTGG AGAAGCTGCA GAGCTCCCTG 
GATACCGTCG GTCAGGAGAG GGAGGAGCTG CAGGAGGAGC TGAGACAGAA GCAGGAAGCT 
GAGATGCAGC TGCAGAGAAG AACGGAGATG TTGGAGGCTG AGGTCAGGGA GCTGAACGCG 
TTTGTACAGA ATGTTACTGA GCAGAAGAGG CAGCAGGAGC AGCAACTGCA GCAACTTGGA 
CAAAAGGCTT CCTCAACAGA AGAACTTCTG AATGCTCTGC AGTATGACTT GCGCCAACAA 
AGAAACGTGA ACTCTGAGCT GAAGAAAGCG AGTGAAGAGG AGAAACGTTC TTTGGAGCAG 
AAGATAACAG AATTATGTAA CAACTTGGAA AGTGTTGAAG CAGAGAGAGA CTTTCATCTG 
TCAGACAAAG AGGAGAACCT GCAGAAGCAC AAAGAAGAGA TGGAGAAGCT GCAGCTCAGC 
GTGACGGCTC TGAGTCAGAA GAGCGATCTG CTGCAGGAGG AGAAGGAGCA GCTCGAGGCG 
CAGCTGCAGC ACAACATCAA CATGGCGTCA GCCTACCAGG AGAAGATCAC CCAGCAGGAG 
CTGCTGATGA CGCAGAAGCA AACGGAAAAA GACGAACAAG AAGCAACATT TCAGCAAAAA 
TGGCAGAACC TGCAGAAGGA GCTGCAGCTG CAGAGGGAGG GAAGAGCTCA GTCTGAAGCC 
CAGCGCGACG CCTCCCAGCA GCTGCTCAAT GAAGCAAACG TGTCTCTGTC AGCCCTGAGA 
CAGCAGCTGA ACAACCTGGA GCAGAAGCCC AATGCACTCT GGGAGACTCT GTCACACCTG 
CAGGACTCTG CTGATCATCT TGATGCAACC TTTGGAAAAT TCCAACAGTT TAGAGAAATG 
TGCACAAAGA CGGCCTCTGA AACCCCGGAG AACGTTCTGA AAGTCCAGAG TTTCCTGAAG 
CTTTCTGACC TGAAGGACCT TCCAAAGTCC AGGCTGGCTG TCTTCGGTGA CGTTTGTGAG 
CTGGGATTTC AAACCTGCAG CAGTTTCACA AAGACTCTTG AACAGCTCAC GGTGCATCCA 
CGTGTTTATA TGAAAACATT TGAGCTGCTG GTGAATATAG ACCTGGCTGT TTTTGAGGAG 
AGACGGCTGC AGGACTTGCT GACGTCCAGA GTTGACGCGC CTAAGTTTTC CCTCAACATC 
GAGGATTTCC ACTCCCGGTG GAATCAGAGG CTCTCTAAGC TTCTGGACAA ACGTCGGCTC 
TACCAGCAGC AAATGGAGAG CATTTTGGAG AGGCTCTGGG CCAACCTGAC TTCATATATC 
GCAGTGCGAC AGGCTGAAGT TCAGGAGAGG GTCAAATTCA ACGAGTCTAT CGAGGCCGCC 
TCCAGCCCAC CGGTCATCAG CGAGCTGGAG CGAATCCTCG GCCAAGAAGC TGAACGGAGG 
CGACTTGTGG TCCAAAACGG GACGATGCCT TTACAGGTCA TCAGTGATGA GCTCAGTAAG 
ATTTTGGCTG AACTAATGCA GATGGATAAA CAGAGAGATG GTCAGCTTAG AGAAGAGAGG 
AGGAAGACGT CATCCCTGCT ACAGCAGCTG GAGGGAACGC CTGCGATACC AAACCTGTCC 
TCAGTCCAGG ACTACCAGCA GCTTCAGCAA GCAGCAGGAG AGCTTACAGC TCTGCAGGAG 
CAGCTAGAGG AGGCGAAGGC TGAAGCCAAA AACATGGGGT CCAACCACAA ACGGACCACT 
CAGCTCCTGC AGACTGAACT GCTGGACACT AAAGTTCTTG TAAAGGAGAA GGAGAACACA 
ATCCTGACCT TAAAGGTCAA ACTGGAAGAG TCTGAAAAGA ATGCATCACT CAGTGCAGCT 
CAGCTGAAAA ACTTCCAGAG CAAACTCCGT GAGGAGGAGG TGAAGCTGGC CTCCATCACA 
CAGGAACACC AGCAAGAGAT GGAGAAGATG AAGACGGTGC TGAGTGTGAA GGAGGAGTCT 
CTGAGGAAGC TGAAGCAGGA TCTGAGATCA CAGCATCAGG GCGAGGAGTC GTTCCTCACA 
GGGAAGGAGC TTCACGCTCG ACTAACCAAC CCCAGAGGTA CGATGATCCA GAGCAGCATT 
CTGTTGGAGA AGACCAAGCT GGAAGAAGAG GTGAAACAGC TCCGGCTGCG GATCACTGAG 
CTGGAAAGCT TGGTTTCCTG CCAGCAGGAG GAGACTACCA AGTGGAAGAA CAGAGCAATC 
AGTCTTAAAG GGAAGAGCAA AGCCGAGCAG GACAGGATGA CCTCCCCCTG TACTCCTAAG 
AAGAGGGCCC TGTGTCCAAC CTCAGACAGC ACCTTGTTGT TCAGCCCCAC TAAAAAAAGT 
TACCTTCCTC CTAAGAGAGC CCTGGATTCT CCTCGGAAAC CTCTGGTCAG TGCAGCCTCG 
CTGCTGGACT CTCCCAAAAG CAGCTTTTTC AATGCAGGTG AAGGTTCAGA GCTGCTGACC 
AGAAACTATC CCACTCAGTT CTTTGATAAC TCCAACCTTG GAATCAAACC CGATGAACCA 
GCTGCTGAAA AACCGGACTT CTGGAACGAT GGCAACTGCA AGACCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cenpe ( XM_023350241.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023206009.1 Xiphophorus maculatus centromere protein E (cenpe), transcript variant X2, mRNA.

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CloneID	OXi27177
Clone ID Related Accession (Same CDS sequence)	XM_023350241.1
Accession Version	XM_023350241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	centromere-associated protein E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036459.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGT CTGCTGTTAA AGTCTGTGTC CGTGTTCGTC CTGTTGCTGA GAGGGAAGAA 
AATGCAGCTT CAGAAAAGCC CGTCCAGCTG TTCTGGGAAA ATGACAAGAA ATCAATTCGT 
CAGATTGATG ATGGAAATTC CAGCAAGTGC TTCACTTTTG ACAGAGTGTT TGGTGCTGAA 
GAATCTACAA GCCAGCTGTA CCAGGACATC GCAAAGCCTC TGGTTGTGTC CACCGTTGCG 
GGTTACAATG GAACCATATT TGCCTACGGT CAGACATCTT CGGGGAAGAC GTTCACAATG 
ATGGGCAGTG ACCGTAACCG TGGAGTCATT CCTCTGGCCG TAGACGACGT CTTCCAAACT 
ATTAAAACGT TTCCAAACAA GGAATTCCTT CTCAGAGTGT CCTACATGGA AATCTACAAC 
GAAACTGTGA CGGACCTGTT GGTTGACAGC TGGAAGAGAA AACCTTTGGA AATTCGGGAA 
ACTATGAATA AAACGGTCTA CGTGGCTGAC CTGACTGAGG AGCTCGTCAC AACGTCTTCA 
CAAGCCCTCG CCTGGATCAG GAAGGGAGAA AAGAACCGTC ACTACGGAAA GACTAAAATG 
AACCAGCGGA GCAGCCGCTC GCACACCATA TTCCGTATGA TCCTGGAGAG CAGAGAAAGG 
AGTGATCCAG CATCTGGGGA AAATGCTGAT GGAGCCATTA TTGTTTCCCA CTTAAATTTA 
GTGGATCTCG CTGGATCTGA GAGAGCCAGT CAAACAGGGA CGGAAGGTAC TCGTTTCAAA 
GAAGGATGCA ATATCAATCT CAGTCTGTTT ATACTTGGAC AGGTGATCAA GAAACTCACT 
GATGAAAACC AGAAAGGCTT TACCAGCTAC AGAGACAGCA AACTGACCCG CATCCTGCAG 
AACTCCCTGG GAGGCAACGC TAAAACGGTC ATCATCAGCA CCATCACGCC CGTTTCTCTG 
GAGGAGACCC TCGGCACTCT GCAGTTTGCC AGCACCGCAA AGAAAATGAA GAACGACCCT 
CATGTGACGG AGGTATCGGA TGACGGCGCG TTGCTCAAGA GGTATCGGAA TGAGATTGTA 
GACCTCAAAC GTCGACTGAA CGAGGTGTCT TCGGTCACAC AGACCACAGT GACGGAAAAA 
GAGGTTCTGT CTCAGCTGCT CCAAGAGAAG GATCAGCTCC AGAGAGAGCA AGAGGACCGG 
ATCAGAAACC TGACCAAACT GCTGGTCACC AGCTCCAACC TGGTCTCTGT TCCAAAGATG 
CCGAAGCGCC GGGTGACGTG GGGAGGAAAG ATGCTCCACG GCAGTCAGCC GGCCTGCGAC 
GACGACGGGA TAGCTAACAT GAACGTGACT TTTAGCAGGA AGAGGAGAGC TAATCGTTTC 
TGTTCCGGGG ATCTAGTTGA AGCTGATGAA GACTTCTACC CTCACTGGGA GATTCCTGAG 
GAGCCTTCAG AAGAGATGGA GATGAGCCAG AGCTCTTTGA CTGTTCGCAG CTTTGGGGAC 
AGTGACCTGG CGTCTCCTGA TCAAAGATGT GCTCTCTTAG AGAAACTATC CAACCTGGAG 
CATCAGCTCG AAACGGAGCG TCAGCAGAGG GAGGAGGCTG TGAGACAGGC CGAAACCCTG 
GACTGCCGAG TCGCAGAGCT GCAGGTCCAG TTGCAGACTT TGCTGAAAGA AGAAGCGGCG 
GAGAAGAAGC AGTCAGAAGA GAGGCTGGCA AACCTGGAGG CGCAGCTGCG AAACGAAACC 
CAGCAGAAGC TGGATGCCTC GTTGCAAGTG CAAGCCGCTG AACAGAAGGT TGCAGAGCTG 
GAGGAGGTCC TGCAAACCGA GGCGCAGCAG AAGCGGGAAG CCACTTTGAC CTCCACTTTT 
TTGGAGATCA AAGTGTCTGA GCTGGAGAGG CAGCTGGAGG AGAAGAGGCA GTCTGAGTCT 
GAGCAGATGA GGAAAGGATT TGCAGAGAGT GTTCAGCTGT GTGAAACCTT GGCATCAGAG 
AAGGAAGCGG TTACTGCCGA GCGAGACCAC CTGAAACAGG AGCTGGGGAT GTTTCTGGAG 
CACACGCAGA GCTTGGAGAA GGAAAATGCT GCTCTCCACC AAGAGCTGAA GGAGAAAAAG 
GATCTGGAAG AGTTCAAAAG TCTGGAGCTG AAGTTCATGG AGGAACAGGA GAGTGAATTA 
CAGAAGGAAA TATCGAGTCT AAAGAAGGCC ATCGAATTGT CTGAACATCA GCGCCTTCAA 
CCAGAGATGA CCCTGACAGC CAGCAAGGAG AATCTGGAGG CTGAGCTGAC TGGACCTGTT 
CTCCACATGG CGGTGGAAAG CCATCCTGAA AAACATCAGA ATGTTTCGTA TGACGACGTT 
GAGCTCAGCG CTCTAGATGA CACGCTGCCA CAAAATCTGT TAGGAAGTGT GAAAGTGGAA 
AGCGGCGTCA TCAGCCTGCA GGAGGAGCTG GCTCTATGTG GAGAGGCAGA GGAGGCACTA 
AGGGAGCAGC AGGAGGAACA GGCTTCACTT CAGACTCCTG GAGATCAGAG TGGAGACCTG 
ACAGGGGAGC TGCAGGATGT TAACAAGACG ACTGACGACC TCCAGTCAGG TCTGGCTGCT 
CAGGAGACGG AGCTGAAGCA ACAATGTGCC GATTTAACTC AGCAGCTCGA TGCACTGCAA 
GCAGAGCAGA GACAGAGCGT CCTGGACAGG AGCCGGCTTT TAGTTTCTCT GGAAGAGAAA 
GACGCAGAGC TGAAGCAGAC GATTGAAGCC CTCCAGGGTG TAGAAGCAGA GAGGGAGGCT 
CTGCTGTTGG AACGACGTGT TCAGAGCTCC GCTGAAGATG GAAACGCAGC GACGGACGTC 
CTCAGGTCCA TTCAGGAAGA ACTCAGGGAA GAAAAGCAGA GGAACTCTGA CATGAAGACG 
GCCTTTCTAG AGAAGGAGTC CGAGTTAAAG GAACATATGC TGAGGCTAAC ACAGGAGCTC 
CAAGATGGCG GCAGGAGCTC CACAGAGGAG GTGGAGAGGC TGCTGACTGC GGTCGCCTCT 
CTGACTGCAG AGAGAGATCA GCTCAAGCAG GACCTGCAGG AAAATGTGGA GCTGATGATC 
GAGAACCAGG ACGAGCTGAG GAGCGTCCTG GAGAAGAACC GTGAGCAGGC CAAGCAGATC 
AAACTTCTGA AGTCTGGAAA CGTCTGCAAG CAGGATGGAG GTCCCACTGA AACGGACACT 
CAGCTGGAGG AAGCACAGGG AAGTATGACC GCTTTAAGTG AGGAGCTGAG GCAAACCCAG 
GCAGAAAAAG ACGGACTGCT CTCCCAGAGC GCAGCCACTC AGACCACAGA GGAGCTGGAG 
GAGCTGCAAG GCAGACTGGC TGCTCTTACC GAGGAGAGAG AGGAGCTGCT GCACGCAGCG 
GAGAGGCTGA GAGGGGAGAA GGAGGAGCTC CGAGCACAGC TGGAGGAGAA GACCCACATG 
ATGCAGTGCG AGATGCAGCA GCAGCAGCTC CTCTCTGAAG CGAATCCACT GAAAGACAAG 
CAGATCCAGC AGCTGGAGGA GCTGGAGCGT CTGAGGTCTG TTCTTCAGGA GAAGGAGAAG 
CTGATGCTTG AGAAGCAGGA GGAGCTCCAG GTGCTGAAGG AGAGCAGGGA GAGGATGGAG 
GAAGATATGA TGGAGCTGAA GAGTCAGCTG GTAGAGATGC AGAGCAGCAG CTGCAACACA 
CACAAGCAGT TTGCTGAGGA GCTGGAGTGT GTGAAAGCAG AGAGGGAGGT TCTGCTGGCC 
CAGAGGGACG CTGTGAGCCA GCGGTTTGAG GAGGAGCTGG AGAAGCTGCA GAGCTCCCTG 
GATACCGTCG GTCAGGAGAG GGAGGAGCTG CAGGAGGAGC TGAGACAGAA GCAGGAAGCT 
GAGATGCAGC TGCAGAGAAG AACGGAGATG TTGGAGGCTG AGGTCAGGGA GCTGAACGCG 
TTTGTACAGA ATGTTACTGA GCAGAAGAGG CAGCAGGAGC AGCAACTGCA GCAACTTGGA 
CAAAAGGCTT CCTCAACAGA AGAACTTCTG AATGCTCTGC AGTATGACTT GCGCCAACAA 
AGAAACGTGA ACTCTGAGCT GAAGAAAGCG AGTGAAGAGG AGAAACGTTC TTTGGAGCAG 
AAGATAACAG AATTATGTAA CAACTTGGAA AGTGTTGAAG CAGAGAGAGA CTTTCATCTG 
TCAGACAAAG AGGAGAACCT GCAGAAGCAC AAAGAAGAGA TGGAGAAGCT GCAGCTCAGC 
GTGACGGCTC TGAGTCAGAA GAGCGATCTG CTGCAGGAGG AGAAGGAGCA GCTCGAGGCG 
CAGCTGCAGC ACAACATCAA CATGGCGTCA GCCTACCAGG AGAAGATCAC CCAGCAGGAG 
CTGCTGATGA CGCAGAAGCA AACGGAAAAA GACGAACAAG AAGCAACATT TCAGCAAAAA 
TGGCAGAACC TGCAGAAGGA GCTGCAGCTG CAGAGGGAGG GAAGAGCTCA GTCTGAAGCC 
CAGCGCGACG CCTCCCAGCA GCTGCTCAAT GAAGCAAACG TGTCTCTGTC AGCCCTGAGA 
CAGCAGCTGA ACAACCTGGA GCAGAAGCCC AATGCACTCT GGGAGACTCT GTCACACCTG 
CAGGACTCTG CTGATCATCT TGATGCAACC TTTGGAAAAT TCCAACAGTT TAGAGAAATG 
TGCACAAAGA CGGCCTCTGA AACCCCGGAG AACGTTCTGA AAGTCCAGAG TTTCCTGAAG 
CTTTCTGACC TGAAGGACCT TCCAAAGTCC AGGCTGGCTG TCTTCGGTGA CGTTTGTGAG 
CTGGGATTTC AAACCTGCAG CAGTTTCACA AAGACTCTTG AACAGCTCAC GGTGCATCCA 
CGTGTTTATA TGAAAACATT TGAGCTGCTG GTGAATATAG ACCTGGCTGT TTTTGAGGAG 
AGACGGCTGC AGGACTTGCT GACGTCCAGA GTTGACGCGC CTAAGTTTTC CCTCAACATC 
GAGGATTTCC ACTCCCGGTG GAATCAGAGG CTCTCTAAGC TTCTGGACAA ACGTCGGCTC 
TACCAGCAGC AAATGGAGAG CATTTTGGAG AGGCTCTGGG CCAACCTGAC TTCATATATC 
GCAGTGCGAC AGGCTGAAGT TCAGGAGAGG GTCAAATTCA ACGAGTCTAT CGAGGCCGCC 
TCCAGCCCAC CGGTCATCAG CGAGCTGGAG CGAATCCTCG GCCAAGAAGC TGAACGGAGG 
CGACTTGTGG TCCAAAACGG GACGATGCCT TTACAGGTCA TCAGTGATGA GCTCAGTAAG 
ATTTTGGCTG AACTAATGCA GATGGATAAA CAGAGAGATG GTCAGCTTAG AGAAGAGAGG 
AGGAAGACGT CATCCCTGCT ACAGCAGCTG GAGGGAACGC CTGCGATACC AAACCTGTCC 
TCAGTCCAGG ACTACCAGCA GCTTCAGCAA GCAGCAGGAG AGCTTACAGC TCTGCAGGAG 
CAGCTAGAGG AGGCGAAGGC TGAAGCCAAA AACATGGGGT CCAACCACAA ACGGACCACT 
CAGCTCCTGC AGACTGAACT GCTGGACACT AAAGTTCTTG TAAAGGAGAA GGAGAACACA 
ATCCTGACCT TAAAGGTCAA ACTGGAAGAG TCTGAAAATG CATCACTCAG TGCAGCTCAG 
CTGAAAAACT TCCAGAGCAA ACTCCGTGAG GAGGAGGTGA AGCTGGCCTC CATCACACAG 
GAACACCAGC AAGAGATGGA GAAGATGAAG ACGGTGCTGA GTGTGAAGGA GGAGTCTCTG 
AGGAAGCTGA AGCAGGATCT GAGATCACAG CATCAGGGCG AGGAGTCGTT CCTCACAGGG 
AAGGAGCTTC ACGCTCGACT AACCAACCCC AGAGGTACGA TGATCCAGAG CAGCATTCTG 
TTGGAGAAGA CCAAGCTGGA AGAAGAGGTG AAACAGCTCC GGCTGCGGAT CACTGAGCTG 
GAAAGCTTGG TTTCCTGCCA GCAGGAGGAG ACTACCAAGT GGAAGAACAG AGCAATCAGT 
CTTAAAGGGA AGAGCAAAGC CGAGCAGGAC AGGATGACCT CCCCCTGTAC TCCTAAGAAG 
AGGGCCCTGT GTCCAACCTC AGACAGCACC TTGTTGTTCA GCCCCACTAA AAAAAGTTAC 
CTTCCTCCTA AGAGAGCCCT GGATTCTCCT CGGAAACCTC TGGTCAGTGC AGCCTCGCTG 
CTGGACTCTC CCAAAAGCAG CTTTTTCAAT GCAGGTGAAG GTTCAGAGCT GCTGACCAGA 
AACTATCCCA CTCAGTTCTT TGATAACTCC AACCTTGGAA TCAAACCCGA TGAACCAGCT 
GCTGAAAAAC CGGACTTCTG GAACGATGGC AACTGCAAGA CCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023206009.1
CDS	223..6510
Translation	MAESAVKVCVRVRPVAEREENAASEKPVQLFWENDKKSIRQIDDGNSSKCFTFDRVFGAEESTSQLYQDIAKPLVVSTVAGYNGTIFAYGQTSSGKTFTMMGSDRNRGVIPLAVDDVFQTIKTFPNKEFLLRVSYMEIYNETVTDLLVDSWKRKPLEIRETMNKTVYVADLTEELVTTSSQALAWIRKGEKNRHYGKTKMNQRSSRSHTIFRMILESRERSDPASGENADGAIIVSHLNLVDLAGSERASQTGTEGTRFKEGCNINLSLFILGQVIKKLTDENQKGFTSYRDSKLTRILQNSLGGNAKTVIISTITPVSLEETLGTLQFASTAKKMKNDPHVTEVSDDGALLKRYRNEIVDLKRRLNEVSSVTQTTVTEKEVLSQLLQEKDQLQREQEDRIRNLTKLLVTSSNLVSVPKMPKRRVTWGGKMLHGSQPACDDDGIANMNVTFSRKRRANRFCSGDLVEADEDFYPHWEIPEEPSEEMEMSQSSLTVRSFGDSDLASPDQRCALLEKLSNLEHQLETERQQREEAVRQAETLDCRVAELQVQLQTLLKEEAAEKKQSEERLANLEAQLRNETQQKLDASLQVQAAEQKVAELEEVLQTEAQQKREATLTSTFLEIKVSELERQLEEKRQSESEQMRKGFAESVQLCETLASEKEAVTAERDHLKQELGMFLEHTQSLEKENAALHQELKEKKDLEEFKSLELKFMEEQESELQKEISSLKKAIELSEHQRLQPEMTLTASKENLEAELTGPVLHMAVESHPEKHQNVSYDDVELSALDDTLPQNLLGSVKVESGVISLQEELALCGEAEEALREQQEEQASLQTPGDQSGDLTGELQDVNKTTDDLQSGLAAQETELKQQCADLTQQLDALQAEQRQSVLDRSRLLVSLEEKDAELKQTIEALQGVEAEREALLLERRVQSSAEDGNAATDVLRSIQEELREEKQRNSDMKTAFLEKESELKEHMLRLTQELQDGGRSSTEEVERLLTAVASLTAERDQLKQDLQENVELMIENQDELRSVLEKNREQAKQIKLLKSGNVCKQDGGPTETDTQLEEAQGSMTALSEELRQTQAEKDGLLSQSAATQTTEELEELQGRLAALTEEREELLHAAERLRGEKEELRAQLEEKTHMMQCEMQQQQLLSEANPLKDKQIQQLEELERLRSVLQEKEKLMLEKQEELQVLKESRERMEEDMMELKSQLVEMQSSSCNTHKQFAEELECVKAEREVLLAQRDAVSQRFEEELEKLQSSLDTVGQEREELQEELRQKQEAEMQLQRRTEMLEAEVRELNAFVQNVTEQKRQQEQQLQQLGQKASSTEELLNALQYDLRQQRNVNSELKKASEEEKRSLEQKITELCNNLESVEAERDFHLSDKEENLQKHKEEMEKLQLSVTALSQKSDLLQEEKEQLEAQLQHNINMASAYQEKITQQELLMTQKQTEKDEQEATFQQKWQNLQKELQLQREGRAQSEAQRDASQQLLNEANVSLSALRQQLNNLEQKPNALWETLSHLQDSADHLDATFGKFQQFREMCTKTASETPENVLKVQSFLKLSDLKDLPKSRLAVFGDVCELGFQTCSSFTKTLEQLTVHPRVYMKTFELLVNIDLAVFEERRLQDLLTSRVDAPKFSLNIEDFHSRWNQRLSKLLDKRRLYQQQMESILERLWANLTSYIAVRQAEVQERVKFNESIEAASSPPVISELERILGQEAERRRLVVQNGTMPLQVISDELSKILAELMQMDKQRDGQLREERRKTSSLLQQLEGTPAIPNLSSVQDYQQLQQAAGELTALQEQLEEAKAEAKNMGSNHKRTTQLLQTELLDTKVLVKEKENTILTLKVKLEESENASLSAAQLKNFQSKLREEEVKLASITQEHQQEMEKMKTVLSVKEESLRKLKQDLRSQHQGEESFLTGKELHARLTNPRGTMIQSSILLEKTKLEEEVKQLRLRITELESLVSCQQEETTKWKNRAISLKGKSKAEQDRMTSPCTPKKRALCPTSDSTLLFSPTKKSYLPPKRALDSPRKPLVSAASLLDSPKSSFFNAGEGSELLTRNYPTQFFDNSNLGIKPDEPAAEKPDFWNDGNCKTQ

Target ORF information:

RefSeq Version	XM_023350241.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus centromere protein E (cenpe), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023350241.1

ORF Insert Sequence:

ATGGCGGAGT CTGCTGTTAA AGTCTGTGTC CGTGTTCGTC CTGTTGCTGA GAGGGAAGAA 
AATGCAGCTT CAGAAAAGCC CGTCCAGCTG TTCTGGGAAA ATGACAAGAA ATCAATTCGT 
CAGATTGATG ATGGAAATTC CAGCAAGTGC TTCACTTTTG ACAGAGTGTT TGGTGCTGAA 
GAATCTACAA GCCAGCTGTA CCAGGACATC GCAAAGCCTC TGGTTGTGTC CACCGTTGCG 
GGTTACAATG GAACCATATT TGCCTACGGT CAGACATCTT CGGGGAAGAC GTTCACAATG 
ATGGGCAGTG ACCGTAACCG TGGAGTCATT CCTCTGGCCG TAGACGACGT CTTCCAAACT 
ATTAAAACGT TTCCAAACAA GGAATTCCTT CTCAGAGTGT CCTACATGGA AATCTACAAC 
GAAACTGTGA CGGACCTGTT GGTTGACAGC TGGAAGAGAA AACCTTTGGA AATTCGGGAA 
ACTATGAATA AAACGGTCTA CGTGGCTGAC CTGACTGAGG AGCTCGTCAC AACGTCTTCA 
CAAGCCCTCG CCTGGATCAG GAAGGGAGAA AAGAACCGTC ACTACGGAAA GACTAAAATG 
AACCAGCGGA GCAGCCGCTC GCACACCATA TTCCGTATGA TCCTGGAGAG CAGAGAAAGG 
AGTGATCCAG CATCTGGGGA AAATGCTGAT GGAGCCATTA TTGTTTCCCA CTTAAATTTA 
GTGGATCTCG CTGGATCTGA GAGAGCCAGT CAAACAGGGA CGGAAGGTAC TCGTTTCAAA 
GAAGGATGCA ATATCAATCT CAGTCTGTTT ATACTTGGAC AGGTGATCAA GAAACTCACT 
GATGAAAACC AGAAAGGCTT TACCAGCTAC AGAGACAGCA AACTGACCCG CATCCTGCAG 
AACTCCCTGG GAGGCAACGC TAAAACGGTC ATCATCAGCA CCATCACGCC CGTTTCTCTG 
GAGGAGACCC TCGGCACTCT GCAGTTTGCC AGCACCGCAA AGAAAATGAA GAACGACCCT 
CATGTGACGG AGGTATCGGA TGACGGCGCG TTGCTCAAGA GGTATCGGAA TGAGATTGTA 
GACCTCAAAC GTCGACTGAA CGAGGTGTCT TCGGTCACAC AGACCACAGT GACGGAAAAA 
GAGGTTCTGT CTCAGCTGCT CCAAGAGAAG GATCAGCTCC AGAGAGAGCA AGAGGACCGG 
ATCAGAAACC TGACCAAACT GCTGGTCACC AGCTCCAACC TGGTCTCTGT TCCAAAGATG 
CCGAAGCGCC GGGTGACGTG GGGAGGAAAG ATGCTCCACG GCAGTCAGCC GGCCTGCGAC 
GACGACGGGA TAGCTAACAT GAACGTGACT TTTAGCAGGA AGAGGAGAGC TAATCGTTTC 
TGTTCCGGGG ATCTAGTTGA AGCTGATGAA GACTTCTACC CTCACTGGGA GATTCCTGAG 
GAGCCTTCAG AAGAGATGGA GATGAGCCAG AGCTCTTTGA CTGTTCGCAG CTTTGGGGAC 
AGTGACCTGG CGTCTCCTGA TCAAAGATGT GCTCTCTTAG AGAAACTATC CAACCTGGAG 
CATCAGCTCG AAACGGAGCG TCAGCAGAGG GAGGAGGCTG TGAGACAGGC CGAAACCCTG 
GACTGCCGAG TCGCAGAGCT GCAGGTCCAG TTGCAGACTT TGCTGAAAGA AGAAGCGGCG 
GAGAAGAAGC AGTCAGAAGA GAGGCTGGCA AACCTGGAGG CGCAGCTGCG AAACGAAACC 
CAGCAGAAGC TGGATGCCTC GTTGCAAGTG CAAGCCGCTG AACAGAAGGT TGCAGAGCTG 
GAGGAGGTCC TGCAAACCGA GGCGCAGCAG AAGCGGGAAG CCACTTTGAC CTCCACTTTT 
TTGGAGATCA AAGTGTCTGA GCTGGAGAGG CAGCTGGAGG AGAAGAGGCA GTCTGAGTCT 
GAGCAGATGA GGAAAGGATT TGCAGAGAGT GTTCAGCTGT GTGAAACCTT GGCATCAGAG 
AAGGAAGCGG TTACTGCCGA GCGAGACCAC CTGAAACAGG AGCTGGGGAT GTTTCTGGAG 
CACACGCAGA GCTTGGAGAA GGAAAATGCT GCTCTCCACC AAGAGCTGAA GGAGAAAAAG 
GATCTGGAAG AGTTCAAAAG TCTGGAGCTG AAGTTCATGG AGGAACAGGA GAGTGAATTA 
CAGAAGGAAA TATCGAGTCT AAAGAAGGCC ATCGAATTGT CTGAACATCA GCGCCTTCAA 
CCAGAGATGA CCCTGACAGC CAGCAAGGAG AATCTGGAGG CTGAGCTGAC TGGACCTGTT 
CTCCACATGG CGGTGGAAAG CCATCCTGAA AAACATCAGA ATGTTTCGTA TGACGACGTT 
GAGCTCAGCG CTCTAGATGA CACGCTGCCA CAAAATCTGT TAGGAAGTGT GAAAGTGGAA 
AGCGGCGTCA TCAGCCTGCA GGAGGAGCTG GCTCTATGTG GAGAGGCAGA GGAGGCACTA 
AGGGAGCAGC AGGAGGAACA GGCTTCACTT CAGACTCCTG GAGATCAGAG TGGAGACCTG 
ACAGGGGAGC TGCAGGATGT TAACAAGACG ACTGACGACC TCCAGTCAGG TCTGGCTGCT 
CAGGAGACGG AGCTGAAGCA ACAATGTGCC GATTTAACTC AGCAGCTCGA TGCACTGCAA 
GCAGAGCAGA GACAGAGCGT CCTGGACAGG AGCCGGCTTT TAGTTTCTCT GGAAGAGAAA 
GACGCAGAGC TGAAGCAGAC GATTGAAGCC CTCCAGGGTG TAGAAGCAGA GAGGGAGGCT 
CTGCTGTTGG AACGACGTGT TCAGAGCTCC GCTGAAGATG GAAACGCAGC GACGGACGTC 
CTCAGGTCCA TTCAGGAAGA ACTCAGGGAA GAAAAGCAGA GGAACTCTGA CATGAAGACG 
GCCTTTCTAG AGAAGGAGTC CGAGTTAAAG GAACATATGC TGAGGCTAAC ACAGGAGCTC 
CAAGATGGCG GCAGGAGCTC CACAGAGGAG GTGGAGAGGC TGCTGACTGC GGTCGCCTCT 
CTGACTGCAG AGAGAGATCA GCTCAAGCAG GACCTGCAGG AAAATGTGGA GCTGATGATC 
GAGAACCAGG ACGAGCTGAG GAGCGTCCTG GAGAAGAACC GTGAGCAGGC CAAGCAGATC 
AAACTTCTGA AGTCTGGAAA CGTCTGCAAG CAGGATGGAG GTCCCACTGA AACGGACACT 
CAGCTGGAGG AAGCACAGGG AAGTATGACC GCTTTAAGTG AGGAGCTGAG GCAAACCCAG 
GCAGAAAAAG ACGGACTGCT CTCCCAGAGC GCAGCCACTC AGACCACAGA GGAGCTGGAG 
GAGCTGCAAG GCAGACTGGC TGCTCTTACC GAGGAGAGAG AGGAGCTGCT GCACGCAGCG 
GAGAGGCTGA GAGGGGAGAA GGAGGAGCTC CGAGCACAGC TGGAGGAGAA GACCCACATG 
ATGCAGTGCG AGATGCAGCA GCAGCAGCTC CTCTCTGAAG CGAATCCACT GAAAGACAAG 
CAGATCCAGC AGCTGGAGGA GCTGGAGCGT CTGAGGTCTG TTCTTCAGGA GAAGGAGAAG 
CTGATGCTTG AGAAGCAGGA GGAGCTCCAG GTGCTGAAGG AGAGCAGGGA GAGGATGGAG 
GAAGATATGA TGGAGCTGAA GAGTCAGCTG GTAGAGATGC AGAGCAGCAG CTGCAACACA 
CACAAGCAGT TTGCTGAGGA GCTGGAGTGT GTGAAAGCAG AGAGGGAGGT TCTGCTGGCC 
CAGAGGGACG CTGTGAGCCA GCGGTTTGAG GAGGAGCTGG AGAAGCTGCA GAGCTCCCTG 
GATACCGTCG GTCAGGAGAG GGAGGAGCTG CAGGAGGAGC TGAGACAGAA GCAGGAAGCT 
GAGATGCAGC TGCAGAGAAG AACGGAGATG TTGGAGGCTG AGGTCAGGGA GCTGAACGCG 
TTTGTACAGA ATGTTACTGA GCAGAAGAGG CAGCAGGAGC AGCAACTGCA GCAACTTGGA 
CAAAAGGCTT CCTCAACAGA AGAACTTCTG AATGCTCTGC AGTATGACTT GCGCCAACAA 
AGAAACGTGA ACTCTGAGCT GAAGAAAGCG AGTGAAGAGG AGAAACGTTC TTTGGAGCAG 
AAGATAACAG AATTATGTAA CAACTTGGAA AGTGTTGAAG CAGAGAGAGA CTTTCATCTG 
TCAGACAAAG AGGAGAACCT GCAGAAGCAC AAAGAAGAGA TGGAGAAGCT GCAGCTCAGC 
GTGACGGCTC TGAGTCAGAA GAGCGATCTG CTGCAGGAGG AGAAGGAGCA GCTCGAGGCG 
CAGCTGCAGC ACAACATCAA CATGGCGTCA GCCTACCAGG AGAAGATCAC CCAGCAGGAG 
CTGCTGATGA CGCAGAAGCA AACGGAAAAA GACGAACAAG AAGCAACATT TCAGCAAAAA 
TGGCAGAACC TGCAGAAGGA GCTGCAGCTG CAGAGGGAGG GAAGAGCTCA GTCTGAAGCC 
CAGCGCGACG CCTCCCAGCA GCTGCTCAAT GAAGCAAACG TGTCTCTGTC AGCCCTGAGA 
CAGCAGCTGA ACAACCTGGA GCAGAAGCCC AATGCACTCT GGGAGACTCT GTCACACCTG 
CAGGACTCTG CTGATCATCT TGATGCAACC TTTGGAAAAT TCCAACAGTT TAGAGAAATG 
TGCACAAAGA CGGCCTCTGA AACCCCGGAG AACGTTCTGA AAGTCCAGAG TTTCCTGAAG 
CTTTCTGACC TGAAGGACCT TCCAAAGTCC AGGCTGGCTG TCTTCGGTGA CGTTTGTGAG 
CTGGGATTTC AAACCTGCAG CAGTTTCACA AAGACTCTTG AACAGCTCAC GGTGCATCCA 
CGTGTTTATA TGAAAACATT TGAGCTGCTG GTGAATATAG ACCTGGCTGT TTTTGAGGAG 
AGACGGCTGC AGGACTTGCT GACGTCCAGA GTTGACGCGC CTAAGTTTTC CCTCAACATC 
GAGGATTTCC ACTCCCGGTG GAATCAGAGG CTCTCTAAGC TTCTGGACAA ACGTCGGCTC 
TACCAGCAGC AAATGGAGAG CATTTTGGAG AGGCTCTGGG CCAACCTGAC TTCATATATC 
GCAGTGCGAC AGGCTGAAGT TCAGGAGAGG GTCAAATTCA ACGAGTCTAT CGAGGCCGCC 
TCCAGCCCAC CGGTCATCAG CGAGCTGGAG CGAATCCTCG GCCAAGAAGC TGAACGGAGG 
CGACTTGTGG TCCAAAACGG GACGATGCCT TTACAGGTCA TCAGTGATGA GCTCAGTAAG 
ATTTTGGCTG AACTAATGCA GATGGATAAA CAGAGAGATG GTCAGCTTAG AGAAGAGAGG 
AGGAAGACGT CATCCCTGCT ACAGCAGCTG GAGGGAACGC CTGCGATACC AAACCTGTCC 
TCAGTCCAGG ACTACCAGCA GCTTCAGCAA GCAGCAGGAG AGCTTACAGC TCTGCAGGAG 
CAGCTAGAGG AGGCGAAGGC TGAAGCCAAA AACATGGGGT CCAACCACAA ACGGACCACT 
CAGCTCCTGC AGACTGAACT GCTGGACACT AAAGTTCTTG TAAAGGAGAA GGAGAACACA 
ATCCTGACCT TAAAGGTCAA ACTGGAAGAG TCTGAAAATG CATCACTCAG TGCAGCTCAG 
CTGAAAAACT TCCAGAGCAA ACTCCGTGAG GAGGAGGTGA AGCTGGCCTC CATCACACAG 
GAACACCAGC AAGAGATGGA GAAGATGAAG ACGGTGCTGA GTGTGAAGGA GGAGTCTCTG 
AGGAAGCTGA AGCAGGATCT GAGATCACAG CATCAGGGCG AGGAGTCGTT CCTCACAGGG 
AAGGAGCTTC ACGCTCGACT AACCAACCCC AGAGGTACGA TGATCCAGAG CAGCATTCTG 
TTGGAGAAGA CCAAGCTGGA AGAAGAGGTG AAACAGCTCC GGCTGCGGAT CACTGAGCTG 
GAAAGCTTGG TTTCCTGCCA GCAGGAGGAG ACTACCAAGT GGAAGAACAG AGCAATCAGT 
CTTAAAGGGA AGAGCAAAGC CGAGCAGGAC AGGATGACCT CCCCCTGTAC TCCTAAGAAG 
AGGGCCCTGT GTCCAACCTC AGACAGCACC TTGTTGTTCA GCCCCACTAA AAAAAGTTAC 
CTTCCTCCTA AGAGAGCCCT GGATTCTCCT CGGAAACCTC TGGTCAGTGC AGCCTCGCTG 
CTGGACTCTC CCAAAAGCAG CTTTTTCAAT GCAGGTGAAG GTTCAGAGCT GCTGACCAGA 
AACTATCCCA CTCAGTTCTT TGATAACTCC AACCTTGGAA TCAAACCCGA TGAACCAGCT 
GCTGAAAAAC CGGACTTCTG GAACGATGGC AACTGCAAGA CCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cenpe cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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