myo18a cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	myo18a
Entrez Gene ID	102230140
Full Name	myosin XVIIIA
Gene Type	protein-coding
Organism	Xiphophorus maculatus(southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023351372.1	XP_023207140.1	unconventional myosin-XVIIIa isoform X6
XM_023351373.1	XP_023207141.1	unconventional myosin-XVIIIa isoform X7
XM_023351367.1	XP_023207135.1	unconventional myosin-XVIIIa isoform X1
XM_023351368.1	XP_023207136.1	unconventional myosin-XVIIIa isoform X2
XM_023351370.1	XP_023207138.1	unconventional myosin-XVIIIa isoform X4
XM_023351369.1	XP_023207137.1	unconventional myosin-XVIIIa isoform X3
XM_005807472.2	XP_005807529.1	unconventional myosin-XVIIIa
XM_023351371.1	XP_023207139.1	unconventional myosin-XVIIIa isoform X5

Pathways from BioSystems
Gene Ontology

The following myo18a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myo18a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXi12778	XM_005807472.2 Latest version!	Xiphophorus maculatus myosin XVIIIA (myo18a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXi28029	XM_023351367.1 Latest version!	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXi28030	XM_023351368.1 Latest version!	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXi28031	XM_023351369.1 Latest version!	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXi28032	XM_023351370.1 Latest version!	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXi28033	XM_023351371.1 Latest version!	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXi28034	XM_023351372.1 Latest version!	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXi28035	XM_023351373.1 Latest version!	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myo18a ( XM_005807472.2 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_005807529.1 Xiphophorus maculatus myosin XVIIIA (myo18a), mRNA.

Price & Availability↑

CloneID	OXi12778
Clone ID Related Accession (Same CDS sequence)	XM_005807472.2
Accession Version	XM_005807472.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1445702400000
Organism	Xiphophorus maculatus(Southern platyfish)
Product	unconventional myosin-XVIIIa
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005372370.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_005807472.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xiphophorus maculatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGTG 
AAGACGGAGG AGCAGATTAC AGCAGAGCAG GCTTGGTACG GATCAGAGAA GGTCTGGCTT 
GTTCACAAAG AGGGCTTCTC TCTCGCCACT GTGCTGAAGA CAGAGTGTGG CTCACTCCCC 
GAGGGAAAGG TGAAGATCAA GCTGGAGCAT GATGGAACAA TACTGGATGT GGATGAGGAT 
GACATAGAAA AGGCCAACCC ACCAACATAT GACCGCTCAG AGGACCTGGC TTCACTCGTT 
TACCTAAATG AGTCCAGCAT GATGCACTGC CTGAGGCAGC GCTACGGAGG AAACCTCATC 
CACTCTTTTG CTGGACCCAA CATGGTCGTT ATCAACCCTC TCAGTACACC TTCTATGTAT 
TCCGAGAAGG TCATGCACAT GTTCAAAGGC TGTCGGCGGG AGGACACTGC CCCTCACATC 
TATGCCGTGG CCCAGTCAGC CTACCGCAAC CTGCTCACTA CCCAGCAGGA TCAGTCCATT 
GTGCTTCTGG GGAAGTCTGG CAGCGGTAAA ACCACCAACT GCCAGCATCT GGTCCAGTAC 
CTGGTCTCTA TAGCTGGAAG CACGGGCAAG ATCTTCTCTG CTGAGAAGTG GCAGGCAGTC 
TACACAATCC TAGAGGCCTT TGGCAACTGC TCCACAACCA TGAACATAAA CGCCAGCCGC 
TTCTCTCATG TTGTGTCCCT GGACTTTGAT CAGGCTGGGC AGGTGGCTTC TGCCTCCATC 
CAGACCATGC TGCTGGACAA ACTGAGGGTG AGCAGAAGGC CTGAAGCAGA GTCCACCTTC 
AATGTGTTTT ACTATGTGAT GGCTGGAGCT GACAGCGCTC TGAGAACCGA GTTGCACCTT 
AACCAGCTGA ATGAAAGCAG TGCTTTTGGG ATTCACTCAC ACTCCAAGAC TGAAGACAAG 
CAGCGGGCTG TGCAGCAGTT CACCAAGCTG CAGGCGGCCA TGAAGGTGCT GGGCATTTCT 
GTGGAGGAAC AGAGGGCTCT CTGGCTTATT CTAGGAGCCA TTTATCACTT AGGGGCCGCA 
GGAGCCACTA AAGATGTGAG TGAAGCAGGA CGCAGGCAGT TTGCCCACCA TGAATGGGCT 
CAGAAGGCAG CCTACCTGCT GGGCTGCACC CTGGATGAGT TGTCCTCTCT GATCTTTAAG 
AACCAGACCC GGGGAATGCA GCCGCTGTTC AGAGGGGGCC CGGATGATGC TGGTACAGGT 
GACAACTCAG GATCTAAATT CACAGCTCTG GAATGTTTGG AGTCCATGGC ATCAGGACTG 
TACTCAGAGG TCTTCACAGT GGTCATCTCT CTCATAAATA GGGCTCTGAA GTCCAGTCAG 
CACTCCCTCT GTTCCATTCT GATCGTGGAC ACGCCGGGTT TCCAGAACCC TCGCCTGGCT 
CAGCAGCAAA GAGGAGCTAC GTTTGAGGAG CTCTGCCATA ACTACACCCA GGAGAGGCTG 
CAGACTCTGT TTCATGAACG GACCTTTGTC CAGGAATTGG AAAGATATAA GGAGGAAAAC 
ATAGAGCTTG TTTTAGATGA CGTAGAGTCC AGTTCCTCCC TGTCTATAGC TGCTATTGAT 
CAAGCCCCAC CACAAGCTCT GGTTCGTACT CTGGCCCGAA TGGAAGAGGC TCGAGGCCTT 
TTGTGGCTGA TGGAGGAGGA GGGGGTTAAA CCTGGCGGAT CAGAGGAGAT CTTGCTGGAG 
AGACTCTTTA GCTACTACAG TTCTGGTCTG GGAGAAAACA AAGAAGCCAG TCTGTTACTG 
CGAGGGGAGA AGTCCCACCT CTTCCTTATG GGCCACTGCC ATGGAACGGA TTGGGTGGAG 
TACAACGTTC AGGGCTGGCT GGGCCATGCT AAGAACAATC CTGCAGCCCA GAGTGCTGCC 
ACTCTGCTCC AGGACTCTCA GAAGAAGAAC ATCAGCGGTC TGTTCCTGGG CCGGTCCAGC 
GGGGCCACAG TGCTGTCAGG CTCCATTGCC GGCCTGGAGG GCAGCTCTCA GCTAGCTCTG 
CGCCGCGCCA CCAGCATGAG GAAAACCTTC ACCACAGGCG TCGCTGCTGT CAAGAAGAAG 
AGTCACTGCA TCCAGGTCAA ACTACAAGTG GATGCCTTGA TTGACATGGT GCGCCGTTCC 
AAGGTCCACT TCGTCCATTG TCTGCTGCCC AAAGCAGAGT CAGTGGGGGG CAGTGACCCC 
CGTGTTCCTC ATGGAGAGAG CTCTGATTCT GGGCTGATGA CTTTAGACCT GGGCCTCCTG 
AGAGCTCAGC TCCGGGGCTC CAAGCTGCTA GATGCCCTGC GCATCTACAG GCAAGGTTAC 
CCAGACCACA TGGTGTTCTC AGAGTTCCGC AGACGCTTCG ATGTTCTAGC ACCACATCTG 
ACCAAAAAAC ATGGCCGCAC CGACGTAGTC CCAGATGAGA AGAGAGCAGT GCAGGAGCTG 
CTGGAGTCCC TAGAGCTGGA GAAGAGCAGC TACCACCTGG GGCTAAGCAA GGTGTTTTTC 
CGAGCTGGGA CTTTGTCCAT GCTGGAGGAG CAGCGTGATG TCCAGACCAG GAGGAACATC 
TCTCTGTTCC AGGCTGCATG CAGAGGCTAT CTGGCCAGAC AGGCATTCAA GAAGAGGAAG 
ATCCAGGATC TTGCCATCCG GTGCATCCAG AAGAACATCA AGAAGAACCG CGGTGTGAAG 
GGCTGGCCAT GGTGGAAGCT CTTCACCACT GTTAGACCTC TGATAGAGGT GCAGCTCACT 
GAGGAGCAGA TCCGTGGAAA GGATGAGGAG ATTCAGCAGC TGAAACAGAA GCTGGAGAAG 
ATGGAGAAGG AGAGGAACGA GCTGAGGCTG AACAGTGACC GACTGGAGAG CAGGATGACA 
GAGCTGTCCA CAGAGCTAGC TGATGAGCGG AACACGGCCG AGTCGGCCTC CCAGCTGCTG 
GAGGCAGAGA CTTCTGAGAG GCTCCGCTTG GAGAAGGACA TGAAGGACCT GCAGACAAAG 
TTTGACAGCA TGAAGAAACA GACGGAGTCC ATGGAGATGG AGGTGATGGA GGCTCGGCTC 
ATCAGGGCTT CTGAACTCAA TGGAGAGATG GATGACGACG ACACAGGAGG AGAGTGGCGG 
TTAAAGTATG AGCGAGCCAT CAGGGAGATC GAGTTCACCA AGAAAAGACT GCAGCAGGAG 
TTTGATGACA AGCTGGAGGT TGAACATCAG AACAAGCGTC AGCTGGAGAG AAGGATCAGT 
GACCTGCAGG CTGATAACGA AGAGTCTCAG CGAAACATTC AGCAGTTAAA GAAAAAGAAT 
CAGCGGCTAG TAGCTGAGCT GCAGGACACC AAGCTTCACC TGGAGGGCCA GCAGAGCCGC 
AACCATGACC TGGAGAAGAA GCAGAGGAAG TTTGACGGTG AGCTGAGTGC AGCTCAGGAC 
GAAGTGCAGA GGGAGAGGAG TCTGAGGGAG AAGTTGGCCC GGGAGAAGGA CATGCAGAGT 
GGGGAGGCCT TCAGCCTCAG ACAGCAGCTA GAGGAGAAGG ACCTTGAATT GCGTACAGTG 
AATTTGAAGC TGGAGCAGGT TGAAGCAGAG CTACAGGATC TTAACTCCCA GGAATCCAAG 
GATGAAGCAT CTCTTGCTAA GCTGAAGAAG CAGCTTCGGG ACATGGAAGC CAAGATCAAG 
GACCAGGAGG AGGAGCTGGA TGAGCAAGCT GGCACCATCC AGATGCTGGA GCAGGCCAAG 
CTGCGACTGG AGATGGAGAT GGAACGTTTG CGTCAGACAC ATTTTAAGGA GATCGAAAGC 
AAGGATGATG AGGTGGAGGA GATCAGGCAG TTATGCAGTA AGAAGTTGAA GCAAATAGAG 
GTCCAGCTTG AAGAAGAGTA TGATGAAAAG CAGAAGGTGC TGAAGGAGAA GAGAGAGCTG 
GAGGCAAAGC TTCTGTCAGC ACAGAGCAAG GTGACAAACT GTGACATAGA CACTGAGAAA 
CGCCTGAAGA AGGACCTGAA GAGGACCAAG GCCCTGCTGG CTGATGCCCA GATCATGTTG 
GACCACATAA AGAACAACGC ACCCAGCAAG AGAGAGATCG CTCAGCTGAA AAACCAGCTG 
GAGGAGTCTG AGTTCACCTG TGCAGCTGCA GTCAAAGCTC GGAAATGCAT GGAGGTGGAG 
ATAGAAGACC TGCATGTTCA GATGGAGGAG ATCTCTAAAG CCAAGCAGAT GCTGGAAGAA 
CAGCAGAGCC GTCTTCAGAG AGAGAAGAAC GACCTGCAGT CCAGGACAGA GGAGGACCAG 
GAGGACCTCA ACGAGCTGAT GAAGAAACAC AAGGCTGCTG TGTCCCAGTT GGCTCAGAAC 
TTGGCTCAAA TGAACGACTT GCAGTCTCAG CTGGAGGAGG CCCTCAAGGA GAAACAGGAG 
GTTCAGGAAA AGATGCAGGT CCTCCAGAGC CAGCTGGAGT TCCAGGAGCA ATCCATGGTT 
GAGAAATCTC TAGCCAATCG TCAGGAGGCC AAGATCCGAG AACTGGAGAC CAAGATGGAG 
TTTGAGAAGT CGCAGGTCAA ACGACTGGAG AATCTTGTAG CTCGTCTGAA GGAGAACGTG 
GAAAAGCTGA CAGAAGAGCG AGACCAGCGC ATCAGCAGCG AGAATCGTGA GAAGGAGCAG 
AATAAGCGAC TGCAGAGACA GATCCGAGAC ATTAAAGAGG AAATGGGGGA ACTGGCCAAA 
AAAGAAGCAG AGGCAAGCCA CAAGAAACAC GAGCTGGAGA TGGACATTGA GAGCCTAGAG 
GCTTCAAACC AGAGCCTGCA GGCGGACCTT AAGCTGGCCT TCAAAAGGAT AGGTGACCTG 
CAGGCGGCCA TCGAGGACGA GATGGAGAGT GATGACAACG AAGACCTTAT AAACAGTTTG 
CAGGATATGG TGGCAAAGTA CCAGAAAAGA AGGAACCGAA TTCAGGGAAA CTCAGACACA 
GACTCTGAAG TGGAGGACCG TGTGGATGGA GTGAAGTCCT GGTTGTCCAA AAATGATGGC 
ACTGTCAAGA ACCTGTCAGA TGAGGACAGC TCCCCCATCC GAAGACGCTC CCACTACGAC 
AGGTCTGATG AGGAAGAGGA AGAAGTGAAA GAGGGCGGTC CGGGGACAAC CAGCTCGTCC 
CGCTCCAGAT ACAGATACAG CAGTCATTTG AAAGATGACG AAGATGATGA TGATGATGAG 
GAGGAGGAGG TGTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005807529.1
CDS	1..6138
Translation	MFSLIKKDKEKEGSKKDKKEKKERMSAAELRSLEEMSMRRGFFNLKREAKRESKHKVEISNPIPIKVASTNELSLTDIESDEFSNRSSMVLDDQISVASSTDDLKGEGGGQDGHRSSVRDRVAHFGSLAKQNSSQMGQMMKRFSFSQRSKEESPSEGSTPSGQNSAAPSPQVESKVFNMLRKHSQRVQPEAADTKKVFIPELVDKIFPAPLQLPTVVAPSAPETREMELQRRNTGDFGFSLRRTTMLDRSPDGGVYRRVVHFAEPGAGTKDLALGLVPGDRLVEINGRNVENKSRDEIVEMIRQSGDAVRLKVQPILELSELSRCWLRNIEGLCREARHVKTEEQITAEQAWYGSEKVWLVHKEGFSLATVLKTECGSLPEGKVKIKLEHDGTILDVDEDDIEKANPPTYDRSEDLASLVYLNESSMMHCLRQRYGGNLIHSFAGPNMVVINPLSTPSMYSEKVMHMFKGCRREDTAPHIYAVAQSAYRNLLTTQQDQSIVLLGKSGSGKTTNCQHLVQYLVSIAGSTGKIFSAEKWQAVYTILEAFGNCSTTMNINASRFSHVVSLDFDQAGQVASASIQTMLLDKLRVSRRPEAESTFNVFYYVMAGADSALRTELHLNQLNESSAFGIHSHSKTEDKQRAVQQFTKLQAAMKVLGISVEEQRALWLILGAIYHLGAAGATKDVSEAGRRQFAHHEWAQKAAYLLGCTLDELSSLIFKNQTRGMQPLFRGGPDDAGTGDNSGSKFTALECLESMASGLYSEVFTVVISLINRALKSSQHSLCSILIVDTPGFQNPRLAQQQRGATFEELCHNYTQERLQTLFHERTFVQELERYKEENIELVLDDVESSSSLSIAAIDQAPPQALVRTLARMEEARGLLWLMEEEGVKPGGSEEILLERLFSYYSSGLGENKEASLLLRGEKSHLFLMGHCHGTDWVEYNVQGWLGHAKNNPAAQSAATLLQDSQKKNISGLFLGRSSGATVLSGSIAGLEGSSQLALRRATSMRKTFTTGVAAVKKKSHCIQVKLQVDALIDMVRRSKVHFVHCLLPKAESVGGSDPRVPHGESSDSGLMTLDLGLLRAQLRGSKLLDALRIYRQGYPDHMVFSEFRRRFDVLAPHLTKKHGRTDVVPDEKRAVQELLESLELEKSSYHLGLSKVFFRAGTLSMLEEQRDVQTRRNISLFQAACRGYLARQAFKKRKIQDLAIRCIQKNIKKNRGVKGWPWWKLFTTVRPLIEVQLTEEQIRGKDEEIQQLKQKLEKMEKERNELRLNSDRLESRMTELSTELADERNTAESASQLLEAETSERLRLEKDMKDLQTKFDSMKKQTESMEMEVMEARLIRASELNGEMDDDDTGGEWRLKYERAIREIEFTKKRLQQEFDDKLEVEHQNKRQLERRISDLQADNEESQRNIQQLKKKNQRLVAELQDTKLHLEGQQSRNHDLEKKQRKFDGELSAAQDEVQRERSLREKLAREKDMQSGEAFSLRQQLEEKDLELRTVNLKLEQVEAELQDLNSQESKDEASLAKLKKQLRDMEAKIKDQEEELDEQAGTIQMLEQAKLRLEMEMERLRQTHFKEIESKDDEVEEIRQLCSKKLKQIEVQLEEEYDEKQKVLKEKRELEAKLLSAQSKVTNCDIDTEKRLKKDLKRTKALLADAQIMLDHIKNNAPSKREIAQLKNQLEESEFTCAAAVKARKCMEVEIEDLHVQMEEISKAKQMLEEQQSRLQREKNDLQSRTEEDQEDLNELMKKHKAAVSQLAQNLAQMNDLQSQLEEALKEKQEVQEKMQVLQSQLEFQEQSMVEKSLANRQEAKIRELETKMEFEKSQVKRLENLVARLKENVEKLTEERDQRISSENREKEQNKRLQRQIRDIKEEMGELAKKEAEASHKKHELEMDIESLEASNQSLQADLKLAFKRIGDLQAAIEDEMESDDNEDLINSLQDMVAKYQKRRNRIQGNSDTDSEVEDRVDGVKSWLSKNDGTVKNLSDEDSSPIRRRSHYDRSDEEEEEVKEGGPGTTSSSRSRYRYSSHLKDDEDDDDDEEEEVS

Target ORF information:

RefSeq Version	XM_005807472.2
Organism	Xiphophorus maculatus(Southern platyfish)
Definition	Xiphophorus maculatus myosin XVIIIA (myo18a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005807472.2

ORF Insert Sequence:

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGTG 
AAGACGGAGG AGCAGATTAC AGCAGAGCAG GCTTGGTACG GATCAGAGAA GGTCTGGCTT 
GTTCACAAAG AGGGCTTCTC TCTCGCCACT GTGCTGAAGA CAGAGTGTGG CTCACTCCCC 
GAGGGAAAGG TGAAGATCAA GCTGGAGCAT GATGGAACAA TACTGGATGT GGATGAGGAT 
GACATAGAAA AGGCCAACCC ACCAACATAT GACCGCTCAG AGGACCTGGC TTCACTCGTT 
TACCTAAATG AGTCCAGCAT GATGCACTGC CTGAGGCAGC GCTACGGAGG AAACCTCATC 
CACTCTTTTG CTGGACCCAA CATGGTCGTT ATCAACCCTC TCAGTACACC TTCTATGTAT 
TCCGAGAAGG TCATGCACAT GTTCAAAGGC TGTCGGCGGG AGGACACTGC CCCTCACATC 
TATGCCGTGG CCCAGTCAGC CTACCGCAAC CTGCTCACTA CCCAGCAGGA TCAGTCCATT 
GTGCTTCTGG GGAAGTCTGG CAGCGGTAAA ACCACCAACT GCCAGCATCT GGTCCAGTAC 
CTGGTCTCTA TAGCTGGAAG CACGGGCAAG ATCTTCTCTG CTGAGAAGTG GCAGGCAGTC 
TACACAATCC TAGAGGCCTT TGGCAACTGC TCCACAACCA TGAACATAAA CGCCAGCCGC 
TTCTCTCATG TTGTGTCCCT GGACTTTGAT CAGGCTGGGC AGGTGGCTTC TGCCTCCATC 
CAGACCATGC TGCTGGACAA ACTGAGGGTG AGCAGAAGGC CTGAAGCAGA GTCCACCTTC 
AATGTGTTTT ACTATGTGAT GGCTGGAGCT GACAGCGCTC TGAGAACCGA GTTGCACCTT 
AACCAGCTGA ATGAAAGCAG TGCTTTTGGG ATTCACTCAC ACTCCAAGAC TGAAGACAAG 
CAGCGGGCTG TGCAGCAGTT CACCAAGCTG CAGGCGGCCA TGAAGGTGCT GGGCATTTCT 
GTGGAGGAAC AGAGGGCTCT CTGGCTTATT CTAGGAGCCA TTTATCACTT AGGGGCCGCA 
GGAGCCACTA AAGATGTGAG TGAAGCAGGA CGCAGGCAGT TTGCCCACCA TGAATGGGCT 
CAGAAGGCAG CCTACCTGCT GGGCTGCACC CTGGATGAGT TGTCCTCTCT GATCTTTAAG 
AACCAGACCC GGGGAATGCA GCCGCTGTTC AGAGGGGGCC CGGATGATGC TGGTACAGGT 
GACAACTCAG GATCTAAATT CACAGCTCTG GAATGTTTGG AGTCCATGGC ATCAGGACTG 
TACTCAGAGG TCTTCACAGT GGTCATCTCT CTCATAAATA GGGCTCTGAA GTCCAGTCAG 
CACTCCCTCT GTTCCATTCT GATCGTGGAC ACGCCGGGTT TCCAGAACCC TCGCCTGGCT 
CAGCAGCAAA GAGGAGCTAC GTTTGAGGAG CTCTGCCATA ACTACACCCA GGAGAGGCTG 
CAGACTCTGT TTCATGAACG GACCTTTGTC CAGGAATTGG AAAGATATAA GGAGGAAAAC 
ATAGAGCTTG TTTTAGATGA CGTAGAGTCC AGTTCCTCCC TGTCTATAGC TGCTATTGAT 
CAAGCCCCAC CACAAGCTCT GGTTCGTACT CTGGCCCGAA TGGAAGAGGC TCGAGGCCTT 
TTGTGGCTGA TGGAGGAGGA GGGGGTTAAA CCTGGCGGAT CAGAGGAGAT CTTGCTGGAG 
AGACTCTTTA GCTACTACAG TTCTGGTCTG GGAGAAAACA AAGAAGCCAG TCTGTTACTG 
CGAGGGGAGA AGTCCCACCT CTTCCTTATG GGCCACTGCC ATGGAACGGA TTGGGTGGAG 
TACAACGTTC AGGGCTGGCT GGGCCATGCT AAGAACAATC CTGCAGCCCA GAGTGCTGCC 
ACTCTGCTCC AGGACTCTCA GAAGAAGAAC ATCAGCGGTC TGTTCCTGGG CCGGTCCAGC 
GGGGCCACAG TGCTGTCAGG CTCCATTGCC GGCCTGGAGG GCAGCTCTCA GCTAGCTCTG 
CGCCGCGCCA CCAGCATGAG GAAAACCTTC ACCACAGGCG TCGCTGCTGT CAAGAAGAAG 
AGTCACTGCA TCCAGGTCAA ACTACAAGTG GATGCCTTGA TTGACATGGT GCGCCGTTCC 
AAGGTCCACT TCGTCCATTG TCTGCTGCCC AAAGCAGAGT CAGTGGGGGG CAGTGACCCC 
CGTGTTCCTC ATGGAGAGAG CTCTGATTCT GGGCTGATGA CTTTAGACCT GGGCCTCCTG 
AGAGCTCAGC TCCGGGGCTC CAAGCTGCTA GATGCCCTGC GCATCTACAG GCAAGGTTAC 
CCAGACCACA TGGTGTTCTC AGAGTTCCGC AGACGCTTCG ATGTTCTAGC ACCACATCTG 
ACCAAAAAAC ATGGCCGCAC CGACGTAGTC CCAGATGAGA AGAGAGCAGT GCAGGAGCTG 
CTGGAGTCCC TAGAGCTGGA GAAGAGCAGC TACCACCTGG GGCTAAGCAA GGTGTTTTTC 
CGAGCTGGGA CTTTGTCCAT GCTGGAGGAG CAGCGTGATG TCCAGACCAG GAGGAACATC 
TCTCTGTTCC AGGCTGCATG CAGAGGCTAT CTGGCCAGAC AGGCATTCAA GAAGAGGAAG 
ATCCAGGATC TTGCCATCCG GTGCATCCAG AAGAACATCA AGAAGAACCG CGGTGTGAAG 
GGCTGGCCAT GGTGGAAGCT CTTCACCACT GTTAGACCTC TGATAGAGGT GCAGCTCACT 
GAGGAGCAGA TCCGTGGAAA GGATGAGGAG ATTCAGCAGC TGAAACAGAA GCTGGAGAAG 
ATGGAGAAGG AGAGGAACGA GCTGAGGCTG AACAGTGACC GACTGGAGAG CAGGATGACA 
GAGCTGTCCA CAGAGCTAGC TGATGAGCGG AACACGGCCG AGTCGGCCTC CCAGCTGCTG 
GAGGCAGAGA CTTCTGAGAG GCTCCGCTTG GAGAAGGACA TGAAGGACCT GCAGACAAAG 
TTTGACAGCA TGAAGAAACA GACGGAGTCC ATGGAGATGG AGGTGATGGA GGCTCGGCTC 
ATCAGGGCTT CTGAACTCAA TGGAGAGATG GATGACGACG ACACAGGAGG AGAGTGGCGG 
TTAAAGTATG AGCGAGCCAT CAGGGAGATC GAGTTCACCA AGAAAAGACT GCAGCAGGAG 
TTTGATGACA AGCTGGAGGT TGAACATCAG AACAAGCGTC AGCTGGAGAG AAGGATCAGT 
GACCTGCAGG CTGATAACGA AGAGTCTCAG CGAAACATTC AGCAGTTAAA GAAAAAGAAT 
CAGCGGCTAG TAGCTGAGCT GCAGGACACC AAGCTTCACC TGGAGGGCCA GCAGAGCCGC 
AACCATGACC TGGAGAAGAA GCAGAGGAAG TTTGACGGTG AGCTGAGTGC AGCTCAGGAC 
GAAGTGCAGA GGGAGAGGAG TCTGAGGGAG AAGTTGGCCC GGGAGAAGGA CATGCAGAGT 
GGGGAGGCCT TCAGCCTCAG ACAGCAGCTA GAGGAGAAGG ACCTTGAATT GCGTACAGTG 
AATTTGAAGC TGGAGCAGGT TGAAGCAGAG CTACAGGATC TTAACTCCCA GGAATCCAAG 
GATGAAGCAT CTCTTGCTAA GCTGAAGAAG CAGCTTCGGG ACATGGAAGC CAAGATCAAG 
GACCAGGAGG AGGAGCTGGA TGAGCAAGCT GGCACCATCC AGATGCTGGA GCAGGCCAAG 
CTGCGACTGG AGATGGAGAT GGAACGTTTG CGTCAGACAC ATTTTAAGGA GATCGAAAGC 
AAGGATGATG AGGTGGAGGA GATCAGGCAG TTATGCAGTA AGAAGTTGAA GCAAATAGAG 
GTCCAGCTTG AAGAAGAGTA TGATGAAAAG CAGAAGGTGC TGAAGGAGAA GAGAGAGCTG 
GAGGCAAAGC TTCTGTCAGC ACAGAGCAAG GTGACAAACT GTGACATAGA CACTGAGAAA 
CGCCTGAAGA AGGACCTGAA GAGGACCAAG GCCCTGCTGG CTGATGCCCA GATCATGTTG 
GACCACATAA AGAACAACGC ACCCAGCAAG AGAGAGATCG CTCAGCTGAA AAACCAGCTG 
GAGGAGTCTG AGTTCACCTG TGCAGCTGCA GTCAAAGCTC GGAAATGCAT GGAGGTGGAG 
ATAGAAGACC TGCATGTTCA GATGGAGGAG ATCTCTAAAG CCAAGCAGAT GCTGGAAGAA 
CAGCAGAGCC GTCTTCAGAG AGAGAAGAAC GACCTGCAGT CCAGGACAGA GGAGGACCAG 
GAGGACCTCA ACGAGCTGAT GAAGAAACAC AAGGCTGCTG TGTCCCAGTT GGCTCAGAAC 
TTGGCTCAAA TGAACGACTT GCAGTCTCAG CTGGAGGAGG CCCTCAAGGA GAAACAGGAG 
GTTCAGGAAA AGATGCAGGT CCTCCAGAGC CAGCTGGAGT TCCAGGAGCA ATCCATGGTT 
GAGAAATCTC TAGCCAATCG TCAGGAGGCC AAGATCCGAG AACTGGAGAC CAAGATGGAG 
TTTGAGAAGT CGCAGGTCAA ACGACTGGAG AATCTTGTAG CTCGTCTGAA GGAGAACGTG 
GAAAAGCTGA CAGAAGAGCG AGACCAGCGC ATCAGCAGCG AGAATCGTGA GAAGGAGCAG 
AATAAGCGAC TGCAGAGACA GATCCGAGAC ATTAAAGAGG AAATGGGGGA ACTGGCCAAA 
AAAGAAGCAG AGGCAAGCCA CAAGAAACAC GAGCTGGAGA TGGACATTGA GAGCCTAGAG 
GCTTCAAACC AGAGCCTGCA GGCGGACCTT AAGCTGGCCT TCAAAAGGAT AGGTGACCTG 
CAGGCGGCCA TCGAGGACGA GATGGAGAGT GATGACAACG AAGACCTTAT AAACAGTTTG 
CAGGATATGG TGGCAAAGTA CCAGAAAAGA AGGAACCGAA TTCAGGGAAA CTCAGACACA 
GACTCTGAAG TGGAGGACCG TGTGGATGGA GTGAAGTCCT GGTTGTCCAA AAATGATGGC 
ACTGTCAAGA ACCTGTCAGA TGAGGACAGC TCCCCCATCC GAAGACGCTC CCACTACGAC 
AGGTCTGATG AGGAAGAGGA AGAAGTGAAA GAGGGCGGTC CGGGGACAAC CAGCTCGTCC 
CGCTCCAGAT ACAGATACAG CAGTCATTTG AAAGATGACG AAGATGATGA TGATGATGAG 
GAGGAGGAGG TGTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo18a ( XM_023351367.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023207135.1 Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X1, mRNA.

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CloneID	OXi28029
Clone ID Related Accession (Same CDS sequence)	XM_023351367.1
Accession Version	XM_023351367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	unconventional myosin-XVIIIa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036460.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGTG 
AAGACGGAGG AGCAGATTAC AGCAGAGCAG GCTTGGTACG GATCAGAGAA GGTCTGGCTT 
GTTCACAAAG AGGGCTTCTC TCTCGCCACT GTGCTGAAGA CAGAGTGTGG CTCACTCCCC 
GAGGGAAAGG TGAAGATCAA GCTGGAGCAT GATGGAACAA TACTGGATGT GGATGAGGAT 
GACATAGAAA AGGCCAACCC ACCAACATAT GACCGCTCAG AGGACCTGGC TTCACTCGTT 
TACCTAAATG AGTCCAGCAT GATGCACTGC CTGAGGCAGC GCTACGGAGG AAACCTCATC 
CACTCTTTTG CTGGACCCAA CATGGTCGTT ATCAACCCTC TCAGTACACC TTCTATGTAT 
TCCGAGAAGG TCATGCACAT GTTCAAAGGC TGTCGGCGGG AGGACACTGC CCCTCACATC 
TATGCCGTGG CCCAGTCAGC CTACCGCAAC CTGCTCACTA CCCAGCAGGA TCAGTCCATT 
GTGCTTCTGG GGAAGTCTGG CAGCGGTAAA ACCACCAACT GCCAGCATCT GGTCCAGTAC 
CTGGTCTCTA TAGCTGGAAG CACGGGCAAG ATCTTCTCTG CTGAGAAGTG GCAGGCAGTC 
TACACAATCC TAGAGGCCTT TGGCAACTGC TCCACAACCA TGAACATAAA CGCCAGCCGC 
TTCTCTCATG TTGTGTCCCT GGACTTTGAT CAGGCTGGGC AGGTGGCTTC TGCCTCCATC 
CAGACCATGC TGCTGGACAA ACTGAGGGTG AGCAGAAGGC CTGAAGCAGA GTCCACCTTC 
AATGTGTTTT ACTATGTGAT GGCTGGAGCT GACAGCGCTC TGAGAACCGA GTTGCACCTT 
AACCAGCTGA ATGAAAGCAG TGCTTTTGGG ATTCACTCAC ACTCCAAGAC TGAAGACAAG 
CAGCGGGCTG TGCAGCAGTT CACCAAGCTG CAGGCGGCCA TGAAGGTGCT GGGCATTTCT 
GTGGAGGAAC AGAGGGCTCT CTGGCTTATT CTAGGAGCCA TTTATCACTT AGGGGCCGCA 
GGAGCCACTA AAGACGAGGA TGAAGCAGGA CGCAGGCAGT TTGCCCACCA TGAATGGGCT 
CAGAAGGCAG CCTACCTGCT GGGCTGCACC CTGGATGAGT TGTCCTCTCT GATCTTTAAG 
AACCAGACCC GGGGAATGCA GCCGCTGTTC AGAGGGGGCC CGGATGATGC TGGTACAGGT 
GACAACTCAG GATCTAAATT CACAGCTCTG GAATGTTTGG AGTCCATGGC ATCAGGACTG 
TACTCAGAGG TCTTCACAGT GGTCATCTCT CTCATAAATA GGGCTCTGAA GTCCAGTCAG 
CACTCCCTCT GTTCCATTCT GATCGTGGAC ACGCCGGGTT TCCAGAACCC TCGCCTGGCT 
CAGCAGCAAA GAGGAGCTAC GTTTGAGGAG CTCTGCCATA ACTACACCCA GGAGAGGCTG 
CAGACTCTGT TTCATGAACG GACCTTTGTC CAGGAATTGG AAAGATATAA GGAGGAAAAC 
ATAGAGCTTG TTTTAGATGA CGTAGAGTCC AGTTCCTCCC TGTCTATAGC TGCTATTGAT 
CAAGCCCCAC CACAAGCTCT GGTTCGTACT CTGGCCCGAA TGGAAGAGGC TCGAGGCCTT 
TTGTGGCTGA TGGAGGAGGA GGGGGTTAAA CCTGGCGGAT CAGAGGAGAT CTTGCTGGAG 
AGACTCTTTA GCTACTACAG TTCTGGTCTG GGAGAAAACA AAGAAGCCAG TCTGTTACTG 
CGAGGGGAGA AGTCCCACCT CTTCCTTATG GGCCACTGCC ATGGAACGGA TTGGGTGGAG 
TACAACGTTC AGGGCTGGCT GGGCCATGCT AAGAACAATC CTGCAGCCCA GAGTGCTGCC 
ACTCTGCTCC AGGACTCTCA GAAGAAGAAC ATCAGCGGTC TGTTCCTGGG CCGGTCCAGC 
GGGGCCACAG TGCTGTCAGG CTCCATTGCC GGCCTGGAGG GCAGCTCTCA GCTAGCTCTG 
CGCCGCGCCA CCAGCATGAG GAAAACCTTC ACCACAGGCG TCGCTGCTGT CAAGAAGAAG 
AGTCACTGCA TCCAGGTCAA ACTACAAGTG GATGCCTTGA TTGACATGGT GCGCCGTTCC 
AAGGTCCACT TCGTCCATTG TCTGCTGCCC AAAGCAGAGT CAGTGGGGGG CAGTGACCCC 
CGTGTTCCTC ATGGAGAGAG CTCTGATTCT GGGCTGATGA CTTTAGACCT GGGCCTCCTG 
AGAGCTCAGC TCCGGGGCTC CAAGCTGCTA GATGCCCTGC GCATCTACAG GCAAGGTTAC 
CCAGACCACA TGGTGTTCTC AGAGTTCCGC AGACGCTTCG ATGTTCTAGC ACCACATCTG 
ACCAAAAAAC ATGGCCGCAC CGACGTAGTC CCAGATGAGA AGAGAGCAGT GCAGGAGCTG 
CTGGAGTCCC TAGAGCTGGA GAAGAGCAGC TACCACCTGG GGCTAAGCAA GGTGTTTTTC 
CGAGCTGGGA CTTTGTCCAT GCTGGAGGAG CAGCGTGATG TCCAGACCAG GAGGAACATC 
TCTCTGTTCC AGGCTGCATG CAGAGGCTAT CTGGCCAGAC AGGCATTCAA GAAGAGGAAG 
ATCCAGGATC TTGCCATCCG GTGCATCCAG AAGAACATCA AGAAGAACCG CGGTGTGAAG 
GGCTGGCCAT GGTGGAAGCT CTTCACCACT GTTAGACCTC TGATAGAGGT GCAGCTCACT 
GAGGAGCAGA TCCGTGGAAA GGATGAGGAG ATTCAGCAGC TGAAACAGAA GCTGGAGAAG 
ATGGAGAAGG AGAGGAACGA GCTGAGGCTG AACAGTGACC GACTGGAGAG CAGGATGACA 
GAGCTGTCCA CAGAGCTAGC TGATGAGCGG AACACGGCCG AGTCGGCCTC CCAGCTGCTG 
GAGGCAGAGA CTTCTGAGAG GCTCCGCTTG GAGAAGGACA TGAAGGACCT GCAGACAAAG 
TTTGACAGCA TGAAGAAACA GACGGAGTCC ATGGAGATGG AGGTGATGGA GGCTCGGCTC 
ATCAGGGCTT CTGAACTCAA TGGAGAGATG GATGACGACG ACACAGCAGG AGGAGAGTGG 
CGGTTAAAGT ATGAGCGAGC CATCAGGGAG ATCGAGTTCA CCAAGAAAAG ACTGCAGCAG 
GAGTTTGATG ACAAGCTGGA GGTTGAACAT CAGAACAAGC GTCAGCTGGA GAGAAGGATC 
AGTGACCTGC AGGCTGATAA CGAAGAGTCT CAGCGAAACA TTCAGCAGTT AAAGAAAAAG 
AATCAGCGGC TAGTAGCTGA GCTGCAGGAC ACCAAGCTTC ACCTGGAGGG CCAGCAGAGC 
CGCAACCATG ACCTGGAGAA GAAGCAGAGG AAGTTTGACG GTGAGCTGAG TGCAGCTCAG 
GACGAAGTGC AGAGGGAGAG GAGTCTGAGG GAGAAGTTGG CCCGGGAGAA GGACATGCAG 
AGTGGGGAGG CCTTCAGCCT CAGACAGCAG CTAGAGGAGA AGGACCTTGA ATTGCGTACA 
GTGAATTTGA AGCTGGAGCA GGTTGAAGCA GAGCTACAGG ATCTTAACTC CCAGGAATCC 
AAGGATGAAG CATCTCTTGC TAAGCTGAAG AAGCAGCTTC GGGACATGGA AGCCAAGATC 
AAGGACCAGG AGGAGGAGCT GGATGAGCAA GCTGGCACCA TCCAGATGCT GGAGCAGGCC 
AAGCTGCGAC TGGAGATGGA GATGGAACGT TTGCGTCAGA CACATTTTAA GGAGATCGAA 
AGCAAGGATG ATGAGGTGGA GGAGATCAGG CAGTTATGCA GTAAGAAGTT GAAGCAAATA 
GAGGTCCAGC TTGAAGAAGA GTATGATGAA AAGCAGAAGG TGCTGAAGGA GAAGAGAGAG 
CTGGAGGCAA AGCTTCTGTC AGCACAGAGC AAGGTGACAA ACTGTGACAT AGACACTGAG 
AAACGCCTGA AGAAGGACCT GAAGAGGACC AAGGCCCTGC TGGCTGATGC CCAGATCATG 
TTGGACCACA TAAAGAACAA CGCACCCAGC AAGAGAGAGA TCGCTCAGCT GAAAAACCAG 
CTGGAGGAGT CTGAGTTCAC CTGTGCAGCT GCAGTCAAAG CTCGGAAATG CATGGAGGTG 
GAGATAGAAG ACCTGCATGT TCAGATGGAG GAGATCTCTA AAGCCAAGCA GATGCTGGAA 
GAACAGCAGA GCCGTCTTCA GAGAGAGAAG AACGACCTGC AGTCCAGGAC AGAGGAGGAC 
CAGGAGGACC TCAACGAGCT GATGAAGAAA CACAAGGCTG CTGTGTCCCA GTTGGCTCAG 
AACTTGGCTC AAATGAACGA CTTGCAGTCT CAGCTGGAGG AGGCCCTCAA GGAGAAACAG 
GAGGTTCAGG AAAAGATGCA GGTCCTCCAG AGCCAGCTGG AGTTCCAGGA GCAATCCATG 
GTTGAGAAAT CTCTAGCCAA TCGTCAGGAG GCCAAGATCC GAGAACTGGA GACCAAGATG 
GAGTTTGAGA AGTCGCAGGT CAAACGACTG GAGAATCTTG TAGCTCGTCT GAAGGAGAAC 
GTGGAAAAGC TGACAGAAGA GCGAGACCAG CGCATCAGCA GCGAGAATCG TGAGAAGGAG 
CAGAATAAGC GACTGCAGAG ACAGATCCGA GACATTAAAG AGGAAATGGG GGAACTGGCC 
AAAAAAGAAG CAGAGGCAAG CCACAAGAAA CACGAGCTGG AGATGGACAT TGAGAGCCTA 
GAGGCTTCAA ACCAGAGCCT GCAGGCGGAC CTTAAGCTGG CCTTCAAAAG GATAGGTGAC 
CTGCAGGCGG CCATCGAGGA CGAGATGGAG AGTGATGACA ACGAAGACCT TATAAACAGT 
TTGCAGGATA TGGTGGCAAA GTACCAGAAA AGAAGGAACC GAATTCAGGG AAACTCAGAC 
ACAGACTCTG AAGTGGAGGA CCGTGTGGAT GGAGTGAAGT CCTGGTTGTC CAAAAATGAT 
GGCACTGTCA AGAACCTGTC AGATGAGGAC AGATGTGCTG TAAAAACCGA CTTAAAGGAA 
GGAAAAGAGT ATAAAGAGGC TAAGGAGAAA CATAAAGAGC AGAGAGAGCG ACAGACAATC 
AGGCCGGTGC CTGTTGTGGC CTCTCTTAGT TATAGAAAAA AACCTGACCT GGATTTCATC 
ATAGACAAAA GTGACTCCCT CCTCCGGTCT ACCAAGAAAG CAGAGTCAGG CAACGTCCCA 
AGCAGATGCA AAACTGAGCT GCTAAACCAG AAATGTCATG TTGACCCTTA TCTAGCCTCT 
TCTAGGAGAC TTCCTCTTTC ACTGGATGAC GTGAGTGACA AACAGTCAGT CATCTCGCAG 
GCTGGTTCAG AGGCTAATGG TAGCATCAGG AAAGGTACGG ACAGCCGTTG GGGTCAGTTT 
AATCTTTCAT TCACTAAGCC AGGATCATCC TCCACCCTTC CTGAGATAAA CCTGAGGAAT 
AAGGTACGTA CTGGCAGCAG CTTGGGGCTA TCAGGCCTAT ACACACACCA TCAAAGCATC 
TCTGGTACAG ACGGGGCCAG CAGCAGCCAG TGCCCTCCCA GTAGGAGATC AAGAAGTCAA 
AGTCCTGGAA GTGTTGGCCA ACTTGATTCC CAGGTATTTC TAGTTGGTAG CAGTTACCTC 
GGTGACGATG GCATTGACCT AGATGACAGT AGAAGTGTGG CCTTAACTGA GAGGTCCATG 
TTCAGTCCTC TGTCTTCCAT TGGTCGCAGT TTATCAATGC CTCCACCCAG AGACAGGCCC 
ACTTCTGCTG ATCTGCCAAT TGATACCGTG GACATTCGGC CGGTCAGCCA TCAGAACTAC 
CTTGACCCTG ACTTGGAGAA AGCCGTCAAT GAAGTGCTGA ATTATAAACC AATAAAATTC 
AAGCGCAGGA GCTTGGAAGA CTCTGAAGAT GATGAAAAAT CCAGGAGTAA TGAAAATGAG 
AGCCGAAAGA TGGAGAATGG AGAGAGGATG TATCCCACCA GAGGTGCTAG ATGTTCTGAA 
TCTGCTGTGG ATTGCAGGAG ATCGTCCTGC AGTACCAGCA GCCATCACCA TGGCAGCAAG 
AGCAAAAGAA AGAGCAAGAA AAAGAGAAGT CACAGCTCTG AGTCCGACAG TTCTGGAGAC 
AATCTTGTTG AAAGGACCAA CTCTAAAAGG TGGCTCAAAA AGTCCCAAAA AATGTCCAAG 
AAAAAAGAGA AGCGTCCATC ATCGTCAGTA TCTTCTGAAT CAGATTCTGA GTCAAACTTT 
ACATCAACTT CTGATGCATC TGCCATTTCC TACCGGAGTT GCAGTAGTGT GAAAACAGCC 
GTGGGTAGAT CGGTTCCCAG TCCAGATGAG GAGGAGACTC TGCCACCCAA CAAGAAGCAG 
CCGATCAGTA AGAAGAATGA GAAACAAAGG AAGAAGAAGG TGAACCACCT GATGATGAAG 
TACTTGTACC GGACAGAGAG TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023207135.1
CDS	188..7594
Translation	MFSLIKKDKEKEGSKKDKKEKKERMSAAELRSLEEMSMRRGFFNLKREAKRESKHKVEISNPIPIKVASTNELSLTDIESDEFSNRSSMVLDDQISVASSTDDLKGEGGGQDGHRSSVRDRVAHFGSLAKQNSSQMGQMMKRFSFSQRSKEESPSEGSTPSGQNSAAPSPQVESKVFNMLRKHSQRVQPEAADTKKVFIPELVDKIFPAPLQLPTVVAPSAPETREMELQRRNTGDFGFSLRRTTMLDRSPDGGVYRRVVHFAEPGAGTKDLALGLVPGDRLVEINGRNVENKSRDEIVEMIRQSGDAVRLKVQPILELSELSRCWLRNIEGLCREARHVKTEEQITAEQAWYGSEKVWLVHKEGFSLATVLKTECGSLPEGKVKIKLEHDGTILDVDEDDIEKANPPTYDRSEDLASLVYLNESSMMHCLRQRYGGNLIHSFAGPNMVVINPLSTPSMYSEKVMHMFKGCRREDTAPHIYAVAQSAYRNLLTTQQDQSIVLLGKSGSGKTTNCQHLVQYLVSIAGSTGKIFSAEKWQAVYTILEAFGNCSTTMNINASRFSHVVSLDFDQAGQVASASIQTMLLDKLRVSRRPEAESTFNVFYYVMAGADSALRTELHLNQLNESSAFGIHSHSKTEDKQRAVQQFTKLQAAMKVLGISVEEQRALWLILGAIYHLGAAGATKDEDEAGRRQFAHHEWAQKAAYLLGCTLDELSSLIFKNQTRGMQPLFRGGPDDAGTGDNSGSKFTALECLESMASGLYSEVFTVVISLINRALKSSQHSLCSILIVDTPGFQNPRLAQQQRGATFEELCHNYTQERLQTLFHERTFVQELERYKEENIELVLDDVESSSSLSIAAIDQAPPQALVRTLARMEEARGLLWLMEEEGVKPGGSEEILLERLFSYYSSGLGENKEASLLLRGEKSHLFLMGHCHGTDWVEYNVQGWLGHAKNNPAAQSAATLLQDSQKKNISGLFLGRSSGATVLSGSIAGLEGSSQLALRRATSMRKTFTTGVAAVKKKSHCIQVKLQVDALIDMVRRSKVHFVHCLLPKAESVGGSDPRVPHGESSDSGLMTLDLGLLRAQLRGSKLLDALRIYRQGYPDHMVFSEFRRRFDVLAPHLTKKHGRTDVVPDEKRAVQELLESLELEKSSYHLGLSKVFFRAGTLSMLEEQRDVQTRRNISLFQAACRGYLARQAFKKRKIQDLAIRCIQKNIKKNRGVKGWPWWKLFTTVRPLIEVQLTEEQIRGKDEEIQQLKQKLEKMEKERNELRLNSDRLESRMTELSTELADERNTAESASQLLEAETSERLRLEKDMKDLQTKFDSMKKQTESMEMEVMEARLIRASELNGEMDDDDTAGGEWRLKYERAIREIEFTKKRLQQEFDDKLEVEHQNKRQLERRISDLQADNEESQRNIQQLKKKNQRLVAELQDTKLHLEGQQSRNHDLEKKQRKFDGELSAAQDEVQRERSLREKLAREKDMQSGEAFSLRQQLEEKDLELRTVNLKLEQVEAELQDLNSQESKDEASLAKLKKQLRDMEAKIKDQEEELDEQAGTIQMLEQAKLRLEMEMERLRQTHFKEIESKDDEVEEIRQLCSKKLKQIEVQLEEEYDEKQKVLKEKRELEAKLLSAQSKVTNCDIDTEKRLKKDLKRTKALLADAQIMLDHIKNNAPSKREIAQLKNQLEESEFTCAAAVKARKCMEVEIEDLHVQMEEISKAKQMLEEQQSRLQREKNDLQSRTEEDQEDLNELMKKHKAAVSQLAQNLAQMNDLQSQLEEALKEKQEVQEKMQVLQSQLEFQEQSMVEKSLANRQEAKIRELETKMEFEKSQVKRLENLVARLKENVEKLTEERDQRISSENREKEQNKRLQRQIRDIKEEMGELAKKEAEASHKKHELEMDIESLEASNQSLQADLKLAFKRIGDLQAAIEDEMESDDNEDLINSLQDMVAKYQKRRNRIQGNSDTDSEVEDRVDGVKSWLSKNDGTVKNLSDEDRCAVKTDLKEGKEYKEAKEKHKEQRERQTIRPVPVVASLSYRKKPDLDFIIDKSDSLLRSTKKAESGNVPSRCKTELLNQKCHVDPYLASSRRLPLSLDDVSDKQSVISQAGSEANGSIRKGTDSRWGQFNLSFTKPGSSSTLPEINLRNKVRTGSSLGLSGLYTHHQSISGTDGASSSQCPPSRRSRSQSPGSVGQLDSQVFLVGSSYLGDDGIDLDDSRSVALTERSMFSPLSSIGRSLSMPPPRDRPTSADLPIDTVDIRPVSHQNYLDPDLEKAVNEVLNYKPIKFKRRSLEDSEDDEKSRSNENESRKMENGERMYPTRGARCSESAVDCRRSSCSTSSHHHGSKSKRKSKKKRSHSSESDSSGDNLVERTNSKRWLKKSQKMSKKKEKRPSSSVSSESDSESNFTSTSDASAISYRSCSSVKTAVGRSVPSPDEEETLPPNKKQPISKKNEKQRKKKVNHLMMKYLYRTESD

Target ORF information:

RefSeq Version	XM_023351367.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023351367.1

ORF Insert Sequence:

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGTG 
AAGACGGAGG AGCAGATTAC AGCAGAGCAG GCTTGGTACG GATCAGAGAA GGTCTGGCTT 
GTTCACAAAG AGGGCTTCTC TCTCGCCACT GTGCTGAAGA CAGAGTGTGG CTCACTCCCC 
GAGGGAAAGG TGAAGATCAA GCTGGAGCAT GATGGAACAA TACTGGATGT GGATGAGGAT 
GACATAGAAA AGGCCAACCC ACCAACATAT GACCGCTCAG AGGACCTGGC TTCACTCGTT 
TACCTAAATG AGTCCAGCAT GATGCACTGC CTGAGGCAGC GCTACGGAGG AAACCTCATC 
CACTCTTTTG CTGGACCCAA CATGGTCGTT ATCAACCCTC TCAGTACACC TTCTATGTAT 
TCCGAGAAGG TCATGCACAT GTTCAAAGGC TGTCGGCGGG AGGACACTGC CCCTCACATC 
TATGCCGTGG CCCAGTCAGC CTACCGCAAC CTGCTCACTA CCCAGCAGGA TCAGTCCATT 
GTGCTTCTGG GGAAGTCTGG CAGCGGTAAA ACCACCAACT GCCAGCATCT GGTCCAGTAC 
CTGGTCTCTA TAGCTGGAAG CACGGGCAAG ATCTTCTCTG CTGAGAAGTG GCAGGCAGTC 
TACACAATCC TAGAGGCCTT TGGCAACTGC TCCACAACCA TGAACATAAA CGCCAGCCGC 
TTCTCTCATG TTGTGTCCCT GGACTTTGAT CAGGCTGGGC AGGTGGCTTC TGCCTCCATC 
CAGACCATGC TGCTGGACAA ACTGAGGGTG AGCAGAAGGC CTGAAGCAGA GTCCACCTTC 
AATGTGTTTT ACTATGTGAT GGCTGGAGCT GACAGCGCTC TGAGAACCGA GTTGCACCTT 
AACCAGCTGA ATGAAAGCAG TGCTTTTGGG ATTCACTCAC ACTCCAAGAC TGAAGACAAG 
CAGCGGGCTG TGCAGCAGTT CACCAAGCTG CAGGCGGCCA TGAAGGTGCT GGGCATTTCT 
GTGGAGGAAC AGAGGGCTCT CTGGCTTATT CTAGGAGCCA TTTATCACTT AGGGGCCGCA 
GGAGCCACTA AAGACGAGGA TGAAGCAGGA CGCAGGCAGT TTGCCCACCA TGAATGGGCT 
CAGAAGGCAG CCTACCTGCT GGGCTGCACC CTGGATGAGT TGTCCTCTCT GATCTTTAAG 
AACCAGACCC GGGGAATGCA GCCGCTGTTC AGAGGGGGCC CGGATGATGC TGGTACAGGT 
GACAACTCAG GATCTAAATT CACAGCTCTG GAATGTTTGG AGTCCATGGC ATCAGGACTG 
TACTCAGAGG TCTTCACAGT GGTCATCTCT CTCATAAATA GGGCTCTGAA GTCCAGTCAG 
CACTCCCTCT GTTCCATTCT GATCGTGGAC ACGCCGGGTT TCCAGAACCC TCGCCTGGCT 
CAGCAGCAAA GAGGAGCTAC GTTTGAGGAG CTCTGCCATA ACTACACCCA GGAGAGGCTG 
CAGACTCTGT TTCATGAACG GACCTTTGTC CAGGAATTGG AAAGATATAA GGAGGAAAAC 
ATAGAGCTTG TTTTAGATGA CGTAGAGTCC AGTTCCTCCC TGTCTATAGC TGCTATTGAT 
CAAGCCCCAC CACAAGCTCT GGTTCGTACT CTGGCCCGAA TGGAAGAGGC TCGAGGCCTT 
TTGTGGCTGA TGGAGGAGGA GGGGGTTAAA CCTGGCGGAT CAGAGGAGAT CTTGCTGGAG 
AGACTCTTTA GCTACTACAG TTCTGGTCTG GGAGAAAACA AAGAAGCCAG TCTGTTACTG 
CGAGGGGAGA AGTCCCACCT CTTCCTTATG GGCCACTGCC ATGGAACGGA TTGGGTGGAG 
TACAACGTTC AGGGCTGGCT GGGCCATGCT AAGAACAATC CTGCAGCCCA GAGTGCTGCC 
ACTCTGCTCC AGGACTCTCA GAAGAAGAAC ATCAGCGGTC TGTTCCTGGG CCGGTCCAGC 
GGGGCCACAG TGCTGTCAGG CTCCATTGCC GGCCTGGAGG GCAGCTCTCA GCTAGCTCTG 
CGCCGCGCCA CCAGCATGAG GAAAACCTTC ACCACAGGCG TCGCTGCTGT CAAGAAGAAG 
AGTCACTGCA TCCAGGTCAA ACTACAAGTG GATGCCTTGA TTGACATGGT GCGCCGTTCC 
AAGGTCCACT TCGTCCATTG TCTGCTGCCC AAAGCAGAGT CAGTGGGGGG CAGTGACCCC 
CGTGTTCCTC ATGGAGAGAG CTCTGATTCT GGGCTGATGA CTTTAGACCT GGGCCTCCTG 
AGAGCTCAGC TCCGGGGCTC CAAGCTGCTA GATGCCCTGC GCATCTACAG GCAAGGTTAC 
CCAGACCACA TGGTGTTCTC AGAGTTCCGC AGACGCTTCG ATGTTCTAGC ACCACATCTG 
ACCAAAAAAC ATGGCCGCAC CGACGTAGTC CCAGATGAGA AGAGAGCAGT GCAGGAGCTG 
CTGGAGTCCC TAGAGCTGGA GAAGAGCAGC TACCACCTGG GGCTAAGCAA GGTGTTTTTC 
CGAGCTGGGA CTTTGTCCAT GCTGGAGGAG CAGCGTGATG TCCAGACCAG GAGGAACATC 
TCTCTGTTCC AGGCTGCATG CAGAGGCTAT CTGGCCAGAC AGGCATTCAA GAAGAGGAAG 
ATCCAGGATC TTGCCATCCG GTGCATCCAG AAGAACATCA AGAAGAACCG CGGTGTGAAG 
GGCTGGCCAT GGTGGAAGCT CTTCACCACT GTTAGACCTC TGATAGAGGT GCAGCTCACT 
GAGGAGCAGA TCCGTGGAAA GGATGAGGAG ATTCAGCAGC TGAAACAGAA GCTGGAGAAG 
ATGGAGAAGG AGAGGAACGA GCTGAGGCTG AACAGTGACC GACTGGAGAG CAGGATGACA 
GAGCTGTCCA CAGAGCTAGC TGATGAGCGG AACACGGCCG AGTCGGCCTC CCAGCTGCTG 
GAGGCAGAGA CTTCTGAGAG GCTCCGCTTG GAGAAGGACA TGAAGGACCT GCAGACAAAG 
TTTGACAGCA TGAAGAAACA GACGGAGTCC ATGGAGATGG AGGTGATGGA GGCTCGGCTC 
ATCAGGGCTT CTGAACTCAA TGGAGAGATG GATGACGACG ACACAGCAGG AGGAGAGTGG 
CGGTTAAAGT ATGAGCGAGC CATCAGGGAG ATCGAGTTCA CCAAGAAAAG ACTGCAGCAG 
GAGTTTGATG ACAAGCTGGA GGTTGAACAT CAGAACAAGC GTCAGCTGGA GAGAAGGATC 
AGTGACCTGC AGGCTGATAA CGAAGAGTCT CAGCGAAACA TTCAGCAGTT AAAGAAAAAG 
AATCAGCGGC TAGTAGCTGA GCTGCAGGAC ACCAAGCTTC ACCTGGAGGG CCAGCAGAGC 
CGCAACCATG ACCTGGAGAA GAAGCAGAGG AAGTTTGACG GTGAGCTGAG TGCAGCTCAG 
GACGAAGTGC AGAGGGAGAG GAGTCTGAGG GAGAAGTTGG CCCGGGAGAA GGACATGCAG 
AGTGGGGAGG CCTTCAGCCT CAGACAGCAG CTAGAGGAGA AGGACCTTGA ATTGCGTACA 
GTGAATTTGA AGCTGGAGCA GGTTGAAGCA GAGCTACAGG ATCTTAACTC CCAGGAATCC 
AAGGATGAAG CATCTCTTGC TAAGCTGAAG AAGCAGCTTC GGGACATGGA AGCCAAGATC 
AAGGACCAGG AGGAGGAGCT GGATGAGCAA GCTGGCACCA TCCAGATGCT GGAGCAGGCC 
AAGCTGCGAC TGGAGATGGA GATGGAACGT TTGCGTCAGA CACATTTTAA GGAGATCGAA 
AGCAAGGATG ATGAGGTGGA GGAGATCAGG CAGTTATGCA GTAAGAAGTT GAAGCAAATA 
GAGGTCCAGC TTGAAGAAGA GTATGATGAA AAGCAGAAGG TGCTGAAGGA GAAGAGAGAG 
CTGGAGGCAA AGCTTCTGTC AGCACAGAGC AAGGTGACAA ACTGTGACAT AGACACTGAG 
AAACGCCTGA AGAAGGACCT GAAGAGGACC AAGGCCCTGC TGGCTGATGC CCAGATCATG 
TTGGACCACA TAAAGAACAA CGCACCCAGC AAGAGAGAGA TCGCTCAGCT GAAAAACCAG 
CTGGAGGAGT CTGAGTTCAC CTGTGCAGCT GCAGTCAAAG CTCGGAAATG CATGGAGGTG 
GAGATAGAAG ACCTGCATGT TCAGATGGAG GAGATCTCTA AAGCCAAGCA GATGCTGGAA 
GAACAGCAGA GCCGTCTTCA GAGAGAGAAG AACGACCTGC AGTCCAGGAC AGAGGAGGAC 
CAGGAGGACC TCAACGAGCT GATGAAGAAA CACAAGGCTG CTGTGTCCCA GTTGGCTCAG 
AACTTGGCTC AAATGAACGA CTTGCAGTCT CAGCTGGAGG AGGCCCTCAA GGAGAAACAG 
GAGGTTCAGG AAAAGATGCA GGTCCTCCAG AGCCAGCTGG AGTTCCAGGA GCAATCCATG 
GTTGAGAAAT CTCTAGCCAA TCGTCAGGAG GCCAAGATCC GAGAACTGGA GACCAAGATG 
GAGTTTGAGA AGTCGCAGGT CAAACGACTG GAGAATCTTG TAGCTCGTCT GAAGGAGAAC 
GTGGAAAAGC TGACAGAAGA GCGAGACCAG CGCATCAGCA GCGAGAATCG TGAGAAGGAG 
CAGAATAAGC GACTGCAGAG ACAGATCCGA GACATTAAAG AGGAAATGGG GGAACTGGCC 
AAAAAAGAAG CAGAGGCAAG CCACAAGAAA CACGAGCTGG AGATGGACAT TGAGAGCCTA 
GAGGCTTCAA ACCAGAGCCT GCAGGCGGAC CTTAAGCTGG CCTTCAAAAG GATAGGTGAC 
CTGCAGGCGG CCATCGAGGA CGAGATGGAG AGTGATGACA ACGAAGACCT TATAAACAGT 
TTGCAGGATA TGGTGGCAAA GTACCAGAAA AGAAGGAACC GAATTCAGGG AAACTCAGAC 
ACAGACTCTG AAGTGGAGGA CCGTGTGGAT GGAGTGAAGT CCTGGTTGTC CAAAAATGAT 
GGCACTGTCA AGAACCTGTC AGATGAGGAC AGATGTGCTG TAAAAACCGA CTTAAAGGAA 
GGAAAAGAGT ATAAAGAGGC TAAGGAGAAA CATAAAGAGC AGAGAGAGCG ACAGACAATC 
AGGCCGGTGC CTGTTGTGGC CTCTCTTAGT TATAGAAAAA AACCTGACCT GGATTTCATC 
ATAGACAAAA GTGACTCCCT CCTCCGGTCT ACCAAGAAAG CAGAGTCAGG CAACGTCCCA 
AGCAGATGCA AAACTGAGCT GCTAAACCAG AAATGTCATG TTGACCCTTA TCTAGCCTCT 
TCTAGGAGAC TTCCTCTTTC ACTGGATGAC GTGAGTGACA AACAGTCAGT CATCTCGCAG 
GCTGGTTCAG AGGCTAATGG TAGCATCAGG AAAGGTACGG ACAGCCGTTG GGGTCAGTTT 
AATCTTTCAT TCACTAAGCC AGGATCATCC TCCACCCTTC CTGAGATAAA CCTGAGGAAT 
AAGGTACGTA CTGGCAGCAG CTTGGGGCTA TCAGGCCTAT ACACACACCA TCAAAGCATC 
TCTGGTACAG ACGGGGCCAG CAGCAGCCAG TGCCCTCCCA GTAGGAGATC AAGAAGTCAA 
AGTCCTGGAA GTGTTGGCCA ACTTGATTCC CAGGTATTTC TAGTTGGTAG CAGTTACCTC 
GGTGACGATG GCATTGACCT AGATGACAGT AGAAGTGTGG CCTTAACTGA GAGGTCCATG 
TTCAGTCCTC TGTCTTCCAT TGGTCGCAGT TTATCAATGC CTCCACCCAG AGACAGGCCC 
ACTTCTGCTG ATCTGCCAAT TGATACCGTG GACATTCGGC CGGTCAGCCA TCAGAACTAC 
CTTGACCCTG ACTTGGAGAA AGCCGTCAAT GAAGTGCTGA ATTATAAACC AATAAAATTC 
AAGCGCAGGA GCTTGGAAGA CTCTGAAGAT GATGAAAAAT CCAGGAGTAA TGAAAATGAG 
AGCCGAAAGA TGGAGAATGG AGAGAGGATG TATCCCACCA GAGGTGCTAG ATGTTCTGAA 
TCTGCTGTGG ATTGCAGGAG ATCGTCCTGC AGTACCAGCA GCCATCACCA TGGCAGCAAG 
AGCAAAAGAA AGAGCAAGAA AAAGAGAAGT CACAGCTCTG AGTCCGACAG TTCTGGAGAC 
AATCTTGTTG AAAGGACCAA CTCTAAAAGG TGGCTCAAAA AGTCCCAAAA AATGTCCAAG 
AAAAAAGAGA AGCGTCCATC ATCGTCAGTA TCTTCTGAAT CAGATTCTGA GTCAAACTTT 
ACATCAACTT CTGATGCATC TGCCATTTCC TACCGGAGTT GCAGTAGTGT GAAAACAGCC 
GTGGGTAGAT CGGTTCCCAG TCCAGATGAG GAGGAGACTC TGCCACCCAA CAAGAAGCAG 
CCGATCAGTA AGAAGAATGA GAAACAAAGG AAGAAGAAGG TGAACCACCT GATGATGAAG 
TACTTGTACC GGACAGAGAG TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo18a ( XM_023351368.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023207136.1 Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X2, mRNA.

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CloneID	OXi28030
Clone ID Related Accession (Same CDS sequence)	XM_023351368.1
Accession Version	XM_023351368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	unconventional myosin-XVIIIa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036460.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGTG 
AAGACGGAGG AGCAGATTAC AGCAGAGCAG GCTTGGTACG GATCAGAGAA GGTCTGGCTT 
GTTCACAAAG AGGGCTTCTC TCTCGCCACT GTGCTGAAGA CAGAGTGTGG CTCACTCCCC 
GAGGGAAAGG TGAAGATCAA GCTGGAGCAT GATGGAACAA TACTGGATGT GGATGAGGAT 
GACATAGAAA AGGCCAACCC ACCAACATAT GACCGCTCAG AGGACCTGGC TTCACTCGTT 
TACCTAAATG AGTCCAGCAT GATGCACTGC CTGAGGCAGC GCTACGGAGG AAACCTCATC 
CACTCTTTTG CTGGACCCAA CATGGTCGTT ATCAACCCTC TCAGTACACC TTCTATGTAT 
TCCGAGAAGG TCATGCACAT GTTCAAAGGC TGTCGGCGGG AGGACACTGC CCCTCACATC 
TATGCCGTGG CCCAGTCAGC CTACCGCAAC CTGCTCACTA CCCAGCAGGA TCAGTCCATT 
GTGCTTCTGG GGAAGTCTGG CAGCGGTAAA ACCACCAACT GCCAGCATCT GGTCCAGTAC 
CTGGTCTCTA TAGCTGGAAG CACGGGCAAG ATCTTCTCTG CTGAGAAGTG GCAGGCAGTC 
TACACAATCC TAGAGGCCTT TGGCAACTGC TCCACAACCA TGAACATAAA CGCCAGCCGC 
TTCTCTCATG TTGTGTCCCT GGACTTTGAT CAGGCTGGGC AGGTGGCTTC TGCCTCCATC 
CAGACCATGC TGCTGGACAA ACTGAGGGTG AGCAGAAGGC CTGAAGCAGA GTCCACCTTC 
AATGTGTTTT ACTATGTGAT GGCTGGAGCT GACAGCGCTC TGAGAACCGA GTTGCACCTT 
AACCAGCTGA ATGAAAGCAG TGCTTTTGGG ATTCACTCAC ACTCCAAGAC TGAAGACAAG 
CAGCGGGCTG TGCAGCAGTT CACCAAGCTG CAGGCGGCCA TGAAGGTGCT GGGCATTTCT 
GTGGAGGAAC AGAGGGCTCT CTGGCTTATT CTAGGAGCCA TTTATCACTT AGGGGCCGCA 
GGAGCCACTA AAGCAGGACG CAGGCAGTTT GCCCACCATG AATGGGCTCA GAAGGCAGCC 
TACCTGCTGG GCTGCACCCT GGATGAGTTG TCCTCTCTGA TCTTTAAGAA CCAGACCCGG 
GGAATGCAGC CGCTGTTCAG AGGGGGCCCG GATGATGCTG GTACAGGTGA CAACTCAGGA 
TCTAAATTCA CAGCTCTGGA ATGTTTGGAG TCCATGGCAT CAGGACTGTA CTCAGAGGTC 
TTCACAGTGG TCATCTCTCT CATAAATAGG GCTCTGAAGT CCAGTCAGCA CTCCCTCTGT 
TCCATTCTGA TCGTGGACAC GCCGGGTTTC CAGAACCCTC GCCTGGCTCA GCAGCAAAGA 
GGAGCTACGT TTGAGGAGCT CTGCCATAAC TACACCCAGG AGAGGCTGCA GACTCTGTTT 
CATGAACGGA CCTTTGTCCA GGAATTGGAA AGATATAAGG AGGAAAACAT AGAGCTTGTT 
TTAGATGACG TAGAGTCCAG TTCCTCCCTG TCTATAGCTG CTATTGATCA AGCCCCACCA 
CAAGCTCTGG TTCGTACTCT GGCCCGAATG GAAGAGGCTC GAGGCCTTTT GTGGCTGATG 
GAGGAGGAGG GGGTTAAACC TGGCGGATCA GAGGAGATCT TGCTGGAGAG ACTCTTTAGC 
TACTACAGTT CTGGTCTGGG AGAAAACAAA GAAGCCAGTC TGTTACTGCG AGGGGAGAAG 
TCCCACCTCT TCCTTATGGG CCACTGCCAT GGAACGGATT GGGTGGAGTA CAACGTTCAG 
GGCTGGCTGG GCCATGCTAA GAACAATCCT GCAGCCCAGA GTGCTGCCAC TCTGCTCCAG 
GACTCTCAGA AGAAGAACAT CAGCGGTCTG TTCCTGGGCC GGTCCAGCGG GGCCACAGTG 
CTGTCAGGCT CCATTGCCGG CCTGGAGGGC AGCTCTCAGC TAGCTCTGCG CCGCGCCACC 
AGCATGAGGA AAACCTTCAC CACAGGCGTC GCTGCTGTCA AGAAGAAGAG TCACTGCATC 
CAGGTCAAAC TACAAGTGGA TGCCTTGATT GACATGGTGC GCCGTTCCAA GGTCCACTTC 
GTCCATTGTC TGCTGCCCAA AGCAGAGTCA GTGGGGGGCA GTGACCCCCG TGTTCCTCAT 
GGAGAGAGCT CTGATTCTGG GCTGATGACT TTAGACCTGG GCCTCCTGAG AGCTCAGCTC 
CGGGGCTCCA AGCTGCTAGA TGCCCTGCGC ATCTACAGGC AAGGTTACCC AGACCACATG 
GTGTTCTCAG AGTTCCGCAG ACGCTTCGAT GTTCTAGCAC CACATCTGAC CAAAAAACAT 
GGCCGCACCG ACGTAGTCCC AGATGAGAAG AGAGCAGTGC AGGAGCTGCT GGAGTCCCTA 
GAGCTGGAGA AGAGCAGCTA CCACCTGGGG CTAAGCAAGG TGTTTTTCCG AGCTGGGACT 
TTGTCCATGC TGGAGGAGCA GCGTGATGTC CAGACCAGGA GGAACATCTC TCTGTTCCAG 
GCTGCATGCA GAGGCTATCT GGCCAGACAG GCATTCAAGA AGAGGAAGAT CCAGGATCTT 
GCCATCCGGT GCATCCAGAA GAACATCAAG AAGAACCGCG GTGTGAAGGG CTGGCCATGG 
TGGAAGCTCT TCACCACTGT TAGACCTCTG ATAGAGGTGC AGCTCACTGA GGAGCAGATC 
CGTGGAAAGG ATGAGGAGAT TCAGCAGCTG AAACAGAAGC TGGAGAAGAT GGAGAAGGAG 
AGGAACGAGC TGAGGCTGAA CAGTGACCGA CTGGAGAGCA GGATGACAGA GCTGTCCACA 
GAGCTAGCTG ATGAGCGGAA CACGGCCGAG TCGGCCTCCC AGCTGCTGGA GGCAGAGACT 
TCTGAGAGGC TCCGCTTGGA GAAGGACATG AAGGACCTGC AGACAAAGTT TGACAGCATG 
AAGAAACAGA CGGAGTCCAT GGAGATGGAG GTGATGGAGG CTCGGCTCAT CAGGGCTTCT 
GAACTCAATG GAGAGATGGA TGACGACGAC ACAGCAGGAG GAGAGTGGCG GTTAAAGTAT 
GAGCGAGCCA TCAGGGAGAT CGAGTTCACC AAGAAAAGAC TGCAGCAGGA GTTTGATGAC 
AAGCTGGAGG TTGAACATCA GAACAAGCGT CAGCTGGAGA GAAGGATCAG TGACCTGCAG 
GCTGATAACG AAGAGTCTCA GCGAAACATT CAGCAGTTAA AGAAAAAGAA TCAGCGGCTA 
GTAGCTGAGC TGCAGGACAC CAAGCTTCAC CTGGAGGGCC AGCAGAGCCG CAACCATGAC 
CTGGAGAAGA AGCAGAGGAA GTTTGACGGT GAGCTGAGTG CAGCTCAGGA CGAAGTGCAG 
AGGGAGAGGA GTCTGAGGGA GAAGTTGGCC CGGGAGAAGG ACATGCAGAG TGGGGAGGCC 
TTCAGCCTCA GACAGCAGCT AGAGGAGAAG GACCTTGAAT TGCGTACAGT GAATTTGAAG 
CTGGAGCAGG TTGAAGCAGA GCTACAGGAT CTTAACTCCC AGGAATCCAA GGATGAAGCA 
TCTCTTGCTA AGCTGAAGAA GCAGCTTCGG GACATGGAAG CCAAGATCAA GGACCAGGAG 
GAGGAGCTGG ATGAGCAAGC TGGCACCATC CAGATGCTGG AGCAGGCCAA GCTGCGACTG 
GAGATGGAGA TGGAACGTTT GCGTCAGACA CATTTTAAGG AGATCGAAAG CAAGGATGAT 
GAGGTGGAGG AGATCAGGCA GTTATGCAGT AAGAAGTTGA AGCAAATAGA GGTCCAGCTT 
GAAGAAGAGT ATGATGAAAA GCAGAAGGTG CTGAAGGAGA AGAGAGAGCT GGAGGCAAAG 
CTTCTGTCAG CACAGAGCAA GGTGACAAAC TGTGACATAG ACACTGAGAA ACGCCTGAAG 
AAGGACCTGA AGAGGACCAA GGCCCTGCTG GCTGATGCCC AGATCATGTT GGACCACATA 
AAGAACAACG CACCCAGCAA GAGAGAGATC GCTCAGCTGA AAAACCAGCT GGAGGAGTCT 
GAGTTCACCT GTGCAGCTGC AGTCAAAGCT CGGAAATGCA TGGAGGTGGA GATAGAAGAC 
CTGCATGTTC AGATGGAGGA GATCTCTAAA GCCAAGCAGA TGCTGGAAGA ACAGCAGAGC 
CGTCTTCAGA GAGAGAAGAA CGACCTGCAG TCCAGGACAG AGGAGGACCA GGAGGACCTC 
AACGAGCTGA TGAAGAAACA CAAGGCTGCT GTGTCCCAGT TGGCTCAGAA CTTGGCTCAA 
ATGAACGACT TGCAGTCTCA GCTGGAGGAG GCCCTCAAGG AGAAACAGGA GGTTCAGGAA 
AAGATGCAGG TCCTCCAGAG CCAGCTGGAG TTCCAGGAGC AATCCATGGT TGAGAAATCT 
CTAGCCAATC GTCAGGAGGC CAAGATCCGA GAACTGGAGA CCAAGATGGA GTTTGAGAAG 
TCGCAGGTCA AACGACTGGA GAATCTTGTA GCTCGTCTGA AGGAGAACGT GGAAAAGCTG 
ACAGAAGAGC GAGACCAGCG CATCAGCAGC GAGAATCGTG AGAAGGAGCA GAATAAGCGA 
CTGCAGAGAC AGATCCGAGA CATTAAAGAG GAAATGGGGG AACTGGCCAA AAAAGAAGCA 
GAGGCAAGCC ACAAGAAACA CGAGCTGGAG ATGGACATTG AGAGCCTAGA GGCTTCAAAC 
CAGAGCCTGC AGGCGGACCT TAAGCTGGCC TTCAAAAGGA TAGGTGACCT GCAGGCGGCC 
ATCGAGGACG AGATGGAGAG TGATGACAAC GAAGACCTTA TAAACAGTTT GCAGGATATG 
GTGGCAAAGT ACCAGAAAAG AAGGAACCGA ATTCAGGGAA ACTCAGACAC AGACTCTGAA 
GTGGAGGACC GTGTGGATGG AGTGAAGTCC TGGTTGTCCA AAAATGATGG CACTGTCAAG 
AACCTGTCAG ATGAGGACAG ATGTGCTGTA AAAACCGACT TAAAGGAAGG AAAAGAGTAT 
AAAGAGGCTA AGGAGAAACA TAAAGAGCAG AGAGAGCGAC AGACAATCAG GCCGGTGCCT 
GTTGTGGCCT CTCTTAGTTA TAGAAAAAAA CCTGACCTGG ATTTCATCAT AGACAAAAGT 
GACTCCCTCC TCCGGTCTAC CAAGAAAGCA GAGTCAGGCA ACGTCCCAAG CAGATGCAAA 
ACTGAGCTGC TAAACCAGAA ATGTCATGTT GACCCTTATC TAGCCTCTTC TAGGAGACTT 
CCTCTTTCAC TGGATGACGT GAGTGACAAA CAGTCAGTCA TCTCGCAGGC TGGTTCAGAG 
GCTAATGGTA GCATCAGGAA AGGTACGGAC AGCCGTTGGG GTCAGTTTAA TCTTTCATTC 
ACTAAGCCAG GATCATCCTC CACCCTTCCT GAGATAAACC TGAGGAATAA GGTACGTACT 
GGCAGCAGCT TGGGGCTATC AGGCCTATAC ACACACCATC AAAGCATCTC TGGTACAGAC 
GGGGCCAGCA GCAGCCAGTG CCCTCCCAGT AGGAGATCAA GAAGTCAAAG TCCTGGAAGT 
GTTGGCCAAC TTGATTCCCA GGTATTTCTA GTTGGTAGCA GTTACCTCGG TGACGATGGC 
ATTGACCTAG ATGACAGTAG AAGTGTGGCC TTAACTGAGA GGTCCATGTT CAGTCCTCTG 
TCTTCCATTG GTCGCAGTTT ATCAATGCCT CCACCCAGAG ACAGGCCCAC TTCTGCTGAT 
CTGCCAATTG ATACCGTGGA CATTCGGCCG GTCAGCCATC AGAACTACCT TGACCCTGAC 
TTGGAGAAAG CCGTCAATGA AGTGCTGAAT TATAAACCAA TAAAATTCAA GCGCAGGAGC 
TTGGAAGACT CTGAAGATGA TGAAAAATCC AGGAGTAATG AAAATGAGAG CCGAAAGATG 
GAGAATGGAG AGAGGATGTA TCCCACCAGA GGTGCTAGAT GTTCTGAATC TGCTGTGGAT 
TGCAGGAGAT CGTCCTGCAG TACCAGCAGC CATCACCATG GCAGCAAGAG CAAAAGAAAG 
AGCAAGAAAA AGAGAAGTCA CAGCTCTGAG TCCGACAGTT CTGGAGACAA TCTTGTTGAA 
AGGACCAACT CTAAAAGGTG GCTCAAAAAG TCCCAAAAAA TGTCCAAGAA AAAAGAGAAG 
CGTCCATCAT CGTCAGTATC TTCTGAATCA GATTCTGAGT CAAACTTTAC ATCAACTTCT 
GATGCATCTG CCATTTCCTA CCGGAGTTGC AGTAGTGTGA AAACAGCCGT GGGTAGATCG 
GTTCCCAGTC CAGATGAGGA GGAGACTCTG CCACCCAACA AGAAGCAGCC GATCAGTAAG 
AAGAATGAGA AACAAAGGAA GAAGAAGGTG AACCACCTGA TGATGAAGTA CTTGTACCGG 
ACAGAGAGTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023207136.1
CDS	188..7582
Translation	MFSLIKKDKEKEGSKKDKKEKKERMSAAELRSLEEMSMRRGFFNLKREAKRESKHKVEISNPIPIKVASTNELSLTDIESDEFSNRSSMVLDDQISVASSTDDLKGEGGGQDGHRSSVRDRVAHFGSLAKQNSSQMGQMMKRFSFSQRSKEESPSEGSTPSGQNSAAPSPQVESKVFNMLRKHSQRVQPEAADTKKVFIPELVDKIFPAPLQLPTVVAPSAPETREMELQRRNTGDFGFSLRRTTMLDRSPDGGVYRRVVHFAEPGAGTKDLALGLVPGDRLVEINGRNVENKSRDEIVEMIRQSGDAVRLKVQPILELSELSRCWLRNIEGLCREARHVKTEEQITAEQAWYGSEKVWLVHKEGFSLATVLKTECGSLPEGKVKIKLEHDGTILDVDEDDIEKANPPTYDRSEDLASLVYLNESSMMHCLRQRYGGNLIHSFAGPNMVVINPLSTPSMYSEKVMHMFKGCRREDTAPHIYAVAQSAYRNLLTTQQDQSIVLLGKSGSGKTTNCQHLVQYLVSIAGSTGKIFSAEKWQAVYTILEAFGNCSTTMNINASRFSHVVSLDFDQAGQVASASIQTMLLDKLRVSRRPEAESTFNVFYYVMAGADSALRTELHLNQLNESSAFGIHSHSKTEDKQRAVQQFTKLQAAMKVLGISVEEQRALWLILGAIYHLGAAGATKAGRRQFAHHEWAQKAAYLLGCTLDELSSLIFKNQTRGMQPLFRGGPDDAGTGDNSGSKFTALECLESMASGLYSEVFTVVISLINRALKSSQHSLCSILIVDTPGFQNPRLAQQQRGATFEELCHNYTQERLQTLFHERTFVQELERYKEENIELVLDDVESSSSLSIAAIDQAPPQALVRTLARMEEARGLLWLMEEEGVKPGGSEEILLERLFSYYSSGLGENKEASLLLRGEKSHLFLMGHCHGTDWVEYNVQGWLGHAKNNPAAQSAATLLQDSQKKNISGLFLGRSSGATVLSGSIAGLEGSSQLALRRATSMRKTFTTGVAAVKKKSHCIQVKLQVDALIDMVRRSKVHFVHCLLPKAESVGGSDPRVPHGESSDSGLMTLDLGLLRAQLRGSKLLDALRIYRQGYPDHMVFSEFRRRFDVLAPHLTKKHGRTDVVPDEKRAVQELLESLELEKSSYHLGLSKVFFRAGTLSMLEEQRDVQTRRNISLFQAACRGYLARQAFKKRKIQDLAIRCIQKNIKKNRGVKGWPWWKLFTTVRPLIEVQLTEEQIRGKDEEIQQLKQKLEKMEKERNELRLNSDRLESRMTELSTELADERNTAESASQLLEAETSERLRLEKDMKDLQTKFDSMKKQTESMEMEVMEARLIRASELNGEMDDDDTAGGEWRLKYERAIREIEFTKKRLQQEFDDKLEVEHQNKRQLERRISDLQADNEESQRNIQQLKKKNQRLVAELQDTKLHLEGQQSRNHDLEKKQRKFDGELSAAQDEVQRERSLREKLAREKDMQSGEAFSLRQQLEEKDLELRTVNLKLEQVEAELQDLNSQESKDEASLAKLKKQLRDMEAKIKDQEEELDEQAGTIQMLEQAKLRLEMEMERLRQTHFKEIESKDDEVEEIRQLCSKKLKQIEVQLEEEYDEKQKVLKEKRELEAKLLSAQSKVTNCDIDTEKRLKKDLKRTKALLADAQIMLDHIKNNAPSKREIAQLKNQLEESEFTCAAAVKARKCMEVEIEDLHVQMEEISKAKQMLEEQQSRLQREKNDLQSRTEEDQEDLNELMKKHKAAVSQLAQNLAQMNDLQSQLEEALKEKQEVQEKMQVLQSQLEFQEQSMVEKSLANRQEAKIRELETKMEFEKSQVKRLENLVARLKENVEKLTEERDQRISSENREKEQNKRLQRQIRDIKEEMGELAKKEAEASHKKHELEMDIESLEASNQSLQADLKLAFKRIGDLQAAIEDEMESDDNEDLINSLQDMVAKYQKRRNRIQGNSDTDSEVEDRVDGVKSWLSKNDGTVKNLSDEDRCAVKTDLKEGKEYKEAKEKHKEQRERQTIRPVPVVASLSYRKKPDLDFIIDKSDSLLRSTKKAESGNVPSRCKTELLNQKCHVDPYLASSRRLPLSLDDVSDKQSVISQAGSEANGSIRKGTDSRWGQFNLSFTKPGSSSTLPEINLRNKVRTGSSLGLSGLYTHHQSISGTDGASSSQCPPSRRSRSQSPGSVGQLDSQVFLVGSSYLGDDGIDLDDSRSVALTERSMFSPLSSIGRSLSMPPPRDRPTSADLPIDTVDIRPVSHQNYLDPDLEKAVNEVLNYKPIKFKRRSLEDSEDDEKSRSNENESRKMENGERMYPTRGARCSESAVDCRRSSCSTSSHHHGSKSKRKSKKKRSHSSESDSSGDNLVERTNSKRWLKKSQKMSKKKEKRPSSSVSSESDSESNFTSTSDASAISYRSCSSVKTAVGRSVPSPDEEETLPPNKKQPISKKNEKQRKKKVNHLMMKYLYRTESD

Target ORF information:

RefSeq Version	XM_023351368.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023351368.1

ORF Insert Sequence:

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGTG 
AAGACGGAGG AGCAGATTAC AGCAGAGCAG GCTTGGTACG GATCAGAGAA GGTCTGGCTT 
GTTCACAAAG AGGGCTTCTC TCTCGCCACT GTGCTGAAGA CAGAGTGTGG CTCACTCCCC 
GAGGGAAAGG TGAAGATCAA GCTGGAGCAT GATGGAACAA TACTGGATGT GGATGAGGAT 
GACATAGAAA AGGCCAACCC ACCAACATAT GACCGCTCAG AGGACCTGGC TTCACTCGTT 
TACCTAAATG AGTCCAGCAT GATGCACTGC CTGAGGCAGC GCTACGGAGG AAACCTCATC 
CACTCTTTTG CTGGACCCAA CATGGTCGTT ATCAACCCTC TCAGTACACC TTCTATGTAT 
TCCGAGAAGG TCATGCACAT GTTCAAAGGC TGTCGGCGGG AGGACACTGC CCCTCACATC 
TATGCCGTGG CCCAGTCAGC CTACCGCAAC CTGCTCACTA CCCAGCAGGA TCAGTCCATT 
GTGCTTCTGG GGAAGTCTGG CAGCGGTAAA ACCACCAACT GCCAGCATCT GGTCCAGTAC 
CTGGTCTCTA TAGCTGGAAG CACGGGCAAG ATCTTCTCTG CTGAGAAGTG GCAGGCAGTC 
TACACAATCC TAGAGGCCTT TGGCAACTGC TCCACAACCA TGAACATAAA CGCCAGCCGC 
TTCTCTCATG TTGTGTCCCT GGACTTTGAT CAGGCTGGGC AGGTGGCTTC TGCCTCCATC 
CAGACCATGC TGCTGGACAA ACTGAGGGTG AGCAGAAGGC CTGAAGCAGA GTCCACCTTC 
AATGTGTTTT ACTATGTGAT GGCTGGAGCT GACAGCGCTC TGAGAACCGA GTTGCACCTT 
AACCAGCTGA ATGAAAGCAG TGCTTTTGGG ATTCACTCAC ACTCCAAGAC TGAAGACAAG 
CAGCGGGCTG TGCAGCAGTT CACCAAGCTG CAGGCGGCCA TGAAGGTGCT GGGCATTTCT 
GTGGAGGAAC AGAGGGCTCT CTGGCTTATT CTAGGAGCCA TTTATCACTT AGGGGCCGCA 
GGAGCCACTA AAGCAGGACG CAGGCAGTTT GCCCACCATG AATGGGCTCA GAAGGCAGCC 
TACCTGCTGG GCTGCACCCT GGATGAGTTG TCCTCTCTGA TCTTTAAGAA CCAGACCCGG 
GGAATGCAGC CGCTGTTCAG AGGGGGCCCG GATGATGCTG GTACAGGTGA CAACTCAGGA 
TCTAAATTCA CAGCTCTGGA ATGTTTGGAG TCCATGGCAT CAGGACTGTA CTCAGAGGTC 
TTCACAGTGG TCATCTCTCT CATAAATAGG GCTCTGAAGT CCAGTCAGCA CTCCCTCTGT 
TCCATTCTGA TCGTGGACAC GCCGGGTTTC CAGAACCCTC GCCTGGCTCA GCAGCAAAGA 
GGAGCTACGT TTGAGGAGCT CTGCCATAAC TACACCCAGG AGAGGCTGCA GACTCTGTTT 
CATGAACGGA CCTTTGTCCA GGAATTGGAA AGATATAAGG AGGAAAACAT AGAGCTTGTT 
TTAGATGACG TAGAGTCCAG TTCCTCCCTG TCTATAGCTG CTATTGATCA AGCCCCACCA 
CAAGCTCTGG TTCGTACTCT GGCCCGAATG GAAGAGGCTC GAGGCCTTTT GTGGCTGATG 
GAGGAGGAGG GGGTTAAACC TGGCGGATCA GAGGAGATCT TGCTGGAGAG ACTCTTTAGC 
TACTACAGTT CTGGTCTGGG AGAAAACAAA GAAGCCAGTC TGTTACTGCG AGGGGAGAAG 
TCCCACCTCT TCCTTATGGG CCACTGCCAT GGAACGGATT GGGTGGAGTA CAACGTTCAG 
GGCTGGCTGG GCCATGCTAA GAACAATCCT GCAGCCCAGA GTGCTGCCAC TCTGCTCCAG 
GACTCTCAGA AGAAGAACAT CAGCGGTCTG TTCCTGGGCC GGTCCAGCGG GGCCACAGTG 
CTGTCAGGCT CCATTGCCGG CCTGGAGGGC AGCTCTCAGC TAGCTCTGCG CCGCGCCACC 
AGCATGAGGA AAACCTTCAC CACAGGCGTC GCTGCTGTCA AGAAGAAGAG TCACTGCATC 
CAGGTCAAAC TACAAGTGGA TGCCTTGATT GACATGGTGC GCCGTTCCAA GGTCCACTTC 
GTCCATTGTC TGCTGCCCAA AGCAGAGTCA GTGGGGGGCA GTGACCCCCG TGTTCCTCAT 
GGAGAGAGCT CTGATTCTGG GCTGATGACT TTAGACCTGG GCCTCCTGAG AGCTCAGCTC 
CGGGGCTCCA AGCTGCTAGA TGCCCTGCGC ATCTACAGGC AAGGTTACCC AGACCACATG 
GTGTTCTCAG AGTTCCGCAG ACGCTTCGAT GTTCTAGCAC CACATCTGAC CAAAAAACAT 
GGCCGCACCG ACGTAGTCCC AGATGAGAAG AGAGCAGTGC AGGAGCTGCT GGAGTCCCTA 
GAGCTGGAGA AGAGCAGCTA CCACCTGGGG CTAAGCAAGG TGTTTTTCCG AGCTGGGACT 
TTGTCCATGC TGGAGGAGCA GCGTGATGTC CAGACCAGGA GGAACATCTC TCTGTTCCAG 
GCTGCATGCA GAGGCTATCT GGCCAGACAG GCATTCAAGA AGAGGAAGAT CCAGGATCTT 
GCCATCCGGT GCATCCAGAA GAACATCAAG AAGAACCGCG GTGTGAAGGG CTGGCCATGG 
TGGAAGCTCT TCACCACTGT TAGACCTCTG ATAGAGGTGC AGCTCACTGA GGAGCAGATC 
CGTGGAAAGG ATGAGGAGAT TCAGCAGCTG AAACAGAAGC TGGAGAAGAT GGAGAAGGAG 
AGGAACGAGC TGAGGCTGAA CAGTGACCGA CTGGAGAGCA GGATGACAGA GCTGTCCACA 
GAGCTAGCTG ATGAGCGGAA CACGGCCGAG TCGGCCTCCC AGCTGCTGGA GGCAGAGACT 
TCTGAGAGGC TCCGCTTGGA GAAGGACATG AAGGACCTGC AGACAAAGTT TGACAGCATG 
AAGAAACAGA CGGAGTCCAT GGAGATGGAG GTGATGGAGG CTCGGCTCAT CAGGGCTTCT 
GAACTCAATG GAGAGATGGA TGACGACGAC ACAGCAGGAG GAGAGTGGCG GTTAAAGTAT 
GAGCGAGCCA TCAGGGAGAT CGAGTTCACC AAGAAAAGAC TGCAGCAGGA GTTTGATGAC 
AAGCTGGAGG TTGAACATCA GAACAAGCGT CAGCTGGAGA GAAGGATCAG TGACCTGCAG 
GCTGATAACG AAGAGTCTCA GCGAAACATT CAGCAGTTAA AGAAAAAGAA TCAGCGGCTA 
GTAGCTGAGC TGCAGGACAC CAAGCTTCAC CTGGAGGGCC AGCAGAGCCG CAACCATGAC 
CTGGAGAAGA AGCAGAGGAA GTTTGACGGT GAGCTGAGTG CAGCTCAGGA CGAAGTGCAG 
AGGGAGAGGA GTCTGAGGGA GAAGTTGGCC CGGGAGAAGG ACATGCAGAG TGGGGAGGCC 
TTCAGCCTCA GACAGCAGCT AGAGGAGAAG GACCTTGAAT TGCGTACAGT GAATTTGAAG 
CTGGAGCAGG TTGAAGCAGA GCTACAGGAT CTTAACTCCC AGGAATCCAA GGATGAAGCA 
TCTCTTGCTA AGCTGAAGAA GCAGCTTCGG GACATGGAAG CCAAGATCAA GGACCAGGAG 
GAGGAGCTGG ATGAGCAAGC TGGCACCATC CAGATGCTGG AGCAGGCCAA GCTGCGACTG 
GAGATGGAGA TGGAACGTTT GCGTCAGACA CATTTTAAGG AGATCGAAAG CAAGGATGAT 
GAGGTGGAGG AGATCAGGCA GTTATGCAGT AAGAAGTTGA AGCAAATAGA GGTCCAGCTT 
GAAGAAGAGT ATGATGAAAA GCAGAAGGTG CTGAAGGAGA AGAGAGAGCT GGAGGCAAAG 
CTTCTGTCAG CACAGAGCAA GGTGACAAAC TGTGACATAG ACACTGAGAA ACGCCTGAAG 
AAGGACCTGA AGAGGACCAA GGCCCTGCTG GCTGATGCCC AGATCATGTT GGACCACATA 
AAGAACAACG CACCCAGCAA GAGAGAGATC GCTCAGCTGA AAAACCAGCT GGAGGAGTCT 
GAGTTCACCT GTGCAGCTGC AGTCAAAGCT CGGAAATGCA TGGAGGTGGA GATAGAAGAC 
CTGCATGTTC AGATGGAGGA GATCTCTAAA GCCAAGCAGA TGCTGGAAGA ACAGCAGAGC 
CGTCTTCAGA GAGAGAAGAA CGACCTGCAG TCCAGGACAG AGGAGGACCA GGAGGACCTC 
AACGAGCTGA TGAAGAAACA CAAGGCTGCT GTGTCCCAGT TGGCTCAGAA CTTGGCTCAA 
ATGAACGACT TGCAGTCTCA GCTGGAGGAG GCCCTCAAGG AGAAACAGGA GGTTCAGGAA 
AAGATGCAGG TCCTCCAGAG CCAGCTGGAG TTCCAGGAGC AATCCATGGT TGAGAAATCT 
CTAGCCAATC GTCAGGAGGC CAAGATCCGA GAACTGGAGA CCAAGATGGA GTTTGAGAAG 
TCGCAGGTCA AACGACTGGA GAATCTTGTA GCTCGTCTGA AGGAGAACGT GGAAAAGCTG 
ACAGAAGAGC GAGACCAGCG CATCAGCAGC GAGAATCGTG AGAAGGAGCA GAATAAGCGA 
CTGCAGAGAC AGATCCGAGA CATTAAAGAG GAAATGGGGG AACTGGCCAA AAAAGAAGCA 
GAGGCAAGCC ACAAGAAACA CGAGCTGGAG ATGGACATTG AGAGCCTAGA GGCTTCAAAC 
CAGAGCCTGC AGGCGGACCT TAAGCTGGCC TTCAAAAGGA TAGGTGACCT GCAGGCGGCC 
ATCGAGGACG AGATGGAGAG TGATGACAAC GAAGACCTTA TAAACAGTTT GCAGGATATG 
GTGGCAAAGT ACCAGAAAAG AAGGAACCGA ATTCAGGGAA ACTCAGACAC AGACTCTGAA 
GTGGAGGACC GTGTGGATGG AGTGAAGTCC TGGTTGTCCA AAAATGATGG CACTGTCAAG 
AACCTGTCAG ATGAGGACAG ATGTGCTGTA AAAACCGACT TAAAGGAAGG AAAAGAGTAT 
AAAGAGGCTA AGGAGAAACA TAAAGAGCAG AGAGAGCGAC AGACAATCAG GCCGGTGCCT 
GTTGTGGCCT CTCTTAGTTA TAGAAAAAAA CCTGACCTGG ATTTCATCAT AGACAAAAGT 
GACTCCCTCC TCCGGTCTAC CAAGAAAGCA GAGTCAGGCA ACGTCCCAAG CAGATGCAAA 
ACTGAGCTGC TAAACCAGAA ATGTCATGTT GACCCTTATC TAGCCTCTTC TAGGAGACTT 
CCTCTTTCAC TGGATGACGT GAGTGACAAA CAGTCAGTCA TCTCGCAGGC TGGTTCAGAG 
GCTAATGGTA GCATCAGGAA AGGTACGGAC AGCCGTTGGG GTCAGTTTAA TCTTTCATTC 
ACTAAGCCAG GATCATCCTC CACCCTTCCT GAGATAAACC TGAGGAATAA GGTACGTACT 
GGCAGCAGCT TGGGGCTATC AGGCCTATAC ACACACCATC AAAGCATCTC TGGTACAGAC 
GGGGCCAGCA GCAGCCAGTG CCCTCCCAGT AGGAGATCAA GAAGTCAAAG TCCTGGAAGT 
GTTGGCCAAC TTGATTCCCA GGTATTTCTA GTTGGTAGCA GTTACCTCGG TGACGATGGC 
ATTGACCTAG ATGACAGTAG AAGTGTGGCC TTAACTGAGA GGTCCATGTT CAGTCCTCTG 
TCTTCCATTG GTCGCAGTTT ATCAATGCCT CCACCCAGAG ACAGGCCCAC TTCTGCTGAT 
CTGCCAATTG ATACCGTGGA CATTCGGCCG GTCAGCCATC AGAACTACCT TGACCCTGAC 
TTGGAGAAAG CCGTCAATGA AGTGCTGAAT TATAAACCAA TAAAATTCAA GCGCAGGAGC 
TTGGAAGACT CTGAAGATGA TGAAAAATCC AGGAGTAATG AAAATGAGAG CCGAAAGATG 
GAGAATGGAG AGAGGATGTA TCCCACCAGA GGTGCTAGAT GTTCTGAATC TGCTGTGGAT 
TGCAGGAGAT CGTCCTGCAG TACCAGCAGC CATCACCATG GCAGCAAGAG CAAAAGAAAG 
AGCAAGAAAA AGAGAAGTCA CAGCTCTGAG TCCGACAGTT CTGGAGACAA TCTTGTTGAA 
AGGACCAACT CTAAAAGGTG GCTCAAAAAG TCCCAAAAAA TGTCCAAGAA AAAAGAGAAG 
CGTCCATCAT CGTCAGTATC TTCTGAATCA GATTCTGAGT CAAACTTTAC ATCAACTTCT 
GATGCATCTG CCATTTCCTA CCGGAGTTGC AGTAGTGTGA AAACAGCCGT GGGTAGATCG 
GTTCCCAGTC CAGATGAGGA GGAGACTCTG CCACCCAACA AGAAGCAGCC GATCAGTAAG 
AAGAATGAGA AACAAAGGAA GAAGAAGGTG AACCACCTGA TGATGAAGTA CTTGTACCGG 
ACAGAGAGTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo18a ( XM_023351369.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023207137.1 Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X3, mRNA.

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CloneID	OXi28031
Clone ID Related Accession (Same CDS sequence)	XM_023351369.1
Accession Version	XM_023351369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	unconventional myosin-XVIIIa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036460.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGAA 
TCAGGGGAAG AGGATCTGTT TTCTCCTTTC CAGACGTCTC ATGTCGCAGT GAAGACGGAG 
GAGCAGATTA CAGCAGAGCA GGCTTGGTAC GGATCAGAGA AGGTCTGGCT TGTTCACAAA 
GAGGGCTTCT CTCTCGCCAC TGTGCTGAAG ACAGAGTGTG GCTCACTCCC CGAGGGAAAG 
GTGAAGATCA AGCTGGAGCA TGATGGAACA ATACTGGATG TGGATGAGGA TGACATAGAA 
AAGGCCAACC CACCAACATA TGACCGCTCA GAGGACCTGG CTTCACTCGT TTACCTAAAT 
GAGTCCAGCA TGATGCACTG CCTGAGGCAG CGCTACGGAG GAAACCTCAT CCACTCTTTT 
GCTGGACCCA ACATGGTCGT TATCAACCCT CTCAGTACAC CTTCTATGTA TTCCGAGAAG 
GTCATGCACA TGTTCAAAGG CTGTCGGCGG GAGGACACTG CCCCTCACAT CTATGCCGTG 
GCCCAGTCAG CCTACCGCAA CCTGCTCACT ACCCAGCAGG ATCAGTCCAT TGTGCTTCTG 
GGGAAGTCTG GCAGCGGTAA AACCACCAAC TGCCAGCATC TGGTCCAGTA CCTGGTCTCT 
ATAGCTGGAA GCACGGGCAA GATCTTCTCT GCTGAGAAGT GGCAGGCAGT CTACACAATC 
CTAGAGGCCT TTGGCAACTG CTCCACAACC ATGAACATAA ACGCCAGCCG CTTCTCTCAT 
GTTGTGTCCC TGGACTTTGA TCAGGCTGGG CAGGTGGCTT CTGCCTCCAT CCAGACCATG 
CTGCTGGACA AACTGAGGGT GAGCAGAAGG CCTGAAGCAG AGTCCACCTT CAATGTGTTT 
TACTATGTGA TGGCTGGAGC TGACAGCGCT CTGAGAACCG AGTTGCACCT TAACCAGCTG 
AATGAAAGCA GTGCTTTTGG GATTCACTCA CACTCCAAGA CTGAAGACAA GCAGCGGGCT 
GTGCAGCAGT TCACCAAGCT GCAGGCGGCC ATGAAGGTGC TGGGCATTTC TGTGGAGGAA 
CAGAGGGCTC TCTGGCTTAT TCTAGGAGCC ATTTATCACT TAGGGGCCGC AGGAGCCACT 
AAAGACGAGG ATGAAGCAGG ACGCAGGCAG TTTGCCCACC ATGAATGGGC TCAGAAGGCA 
GCCTACCTGC TGGGCTGCAC CCTGGATGAG TTGTCCTCTC TGATCTTTAA GAACCAGACC 
CGGGGAATGC AGCCGCTGTT CAGAGGGGGC CCGGATGATG CTGGTACAGG TGACAACTCA 
GGATCTAAAT TCACAGCTCT GGAATGTTTG GAGTCCATGG CATCAGGACT GTACTCAGAG 
GTCTTCACAG TGGTCATCTC TCTCATAAAT AGGGCTCTGA AGTCCAGTCA GCACTCCCTC 
TGTTCCATTC TGATCGTGGA CACGCCGGGT TTCCAGAACC CTCGCCTGGC TCAGCAGCAA 
AGAGGAGCTA CGTTTGAGGA GCTCTGCCAT AACTACACCC AGGAGAGGCT GCAGACTCTG 
TTTCATGAAC GGACCTTTGT CCAGGAATTG GAAAGATATA AGGAGGAAAA CATAGAGCTT 
GTTTTAGATG ACGTAGAGTC CAGTTCCTCC CTGTCTATAG CTGCTATTGA TCAAGCCCCA 
CCACAAGCTC TGGTTCGTAC TCTGGCCCGA ATGGAAGAGG CTCGAGGCCT TTTGTGGCTG 
ATGGAGGAGG AGGGGGTTAA ACCTGGCGGA TCAGAGGAGA TCTTGCTGGA GAGACTCTTT 
AGCTACTACA GTTCTGGTCT GGGAGAAAAC AAAGAAGCCA GTCTGTTACT GCGAGGGGAG 
AAGTCCCACC TCTTCCTTAT GGGCCACTGC CATGGAACGG ATTGGGTGGA GTACAACGTT 
CAGGGCTGGC TGGGCCATGC TAAGAACAAT CCTGCAGCCC AGAGTGCTGC CACTCTGCTC 
CAGGACTCTC AGAAGAAGAA CATCAGCGGT CTGTTCCTGG GCCGGTCCAG CGGGGCCACA 
GTGCTGTCAG GCTCCATTGC CGGCCTGGAG GGCAGCTCTC AGCTAGCTCT GCGCCGCGCC 
ACCAGCATGA GGAAAACCTT CACCACAGGC GTCGCTGCTG TCAAGAAGAA GAGTCACTGC 
ATCCAGGTCA AACTACAAGT GGATGCCTTG ATTGACATGG TGCGCCGTTC CAAGGTCCAC 
TTCGTCCATT GTCTGCTGCC CAAAGCAGAG TCAGTGGGGG GCAGTGACCC CCGTGTTCCT 
CATGGAGAGA GCTCTGATTC TGGGCTGATG ACTTTAGACC TGGGCCTCCT GAGAGCTCAG 
CTCCGGGGCT CCAAGCTGCT AGATGCCCTG CGCATCTACA GGCAAGGTTA CCCAGACCAC 
ATGGTGTTCT CAGAGTTCCG CAGACGCTTC GATGTTCTAG CACCACATCT GACCAAAAAA 
CATGGCCGCA CCGACGTAGT CCCAGATGAG AAGAGAGCAG TGCAGGAGCT GCTGGAGTCC 
CTAGAGCTGG AGAAGAGCAG CTACCACCTG GGGCTAAGCA AGGTGTTTTT CCGAGCTGGG 
ACTTTGTCCA TGCTGGAGGA GCAGCGTGAT GTCCAGACCA GGAGGAACAT CTCTCTGTTC 
CAGGCTGCAT GCAGAGGCTA TCTGGCCAGA CAGGCATTCA AGAAGAGGAA GATCCAGGAT 
CTTGCCATCC GGTGCATCCA GAAGAACATC AAGAAGAACC GCGGTGTGAA GGGCTGGCCA 
TGGTGGAAGC TCTTCACCAC TGTTAGACCT CTGATAGAGG TGCAGCTCAC TGAGGAGCAG 
ATCCGTGGAA AGGATGAGGA GATTCAGCAG CTGAAACAGA AGCTGGAGAA GATGGAGAAG 
GAGAGGAACG AGCTGAGGCT GAACAGTGAC CGACTGGAGA GCAGGATGAC AGAGCTGTCC 
ACAGAGCTAG CTGATGAGCG GAACACGGCC GAGTCGGCCT CCCAGCTGCT GGAGGCAGAG 
ACTTCTGAGA GGCTCCGCTT GGAGAAGGAC ATGAAGGACC TGCAGACAAA GTTTGACAGC 
ATGAAGAAAC AGACGGAGTC CATGGAGATG GAGGTGATGG AGGCTCGGCT CATCAGGGCT 
TCTGAACTCA ATGGAGAGAT GGATGACGAC GACACAGCAG GAGGAGAGTG GCGGTTAAAG 
TATGAGCGAG CCATCAGGGA GATCGAGTTC ACCAAGAAAA GACTGCAGCA GGAGTTTGAT 
GACAAGCTGG AGGTTGAACA TCAGAACAAG CGTCAGCTGG AGAGAAGGAT CAGTGACCTG 
CAGGCTGATA ACGAAGAGTC TCAGCGAAAC ATTCAGCAGT TAAAGAAAAA GAATCAGCGG 
CTAGTAGCTG AGCTGCAGGA CACCAAGCTT CACCTGGAGG GCCAGCAGAG CCGCAACCAT 
GACCTGGAGA AGAAGCAGAG GAAGTTTGAC GGTGAGCTGA GTGCAGCTCA GGACGAAGTG 
CAGAGGGAGA GGAGTCTGAG GGAGAAGTTG GCCCGGGAGA AGGACATGCA GAGTGGGGAG 
GCCTTCAGCC TCAGACAGCA GCTAGAGGAG AAGGACCTTG AATTGCGTAC AGTGAATTTG 
AAGCTGGAGC AGGTTGAAGC AGAGCTACAG GATCTTAACT CCCAGGAATC CAAGGATGAA 
GCATCTCTTG CTAAGCTGAA GAAGCAGCTT CGGGACATGG AAGCCAAGAT CAAGGACCAG 
GAGGAGGAGC TGGATGAGCA AGCTGGCACC ATCCAGATGC TGGAGCAGGC CAAGCTGCGA 
CTGGAGATGG AGATGGAACG TTTGCGTCAG ACACATTTTA AGGAGATCGA AAGCAAGGAT 
GATGAGGTGG AGGAGATCAG GCAGTTATGC AGTAAGAAGT TGAAGCAAAT AGAGGTCCAG 
CTTGAAGAAG AGTATGATGA AAAGCAGAAG GTGCTGAAGG AGAAGAGAGA GCTGGAGGCA 
AAGCTTCTGT CAGCACAGAG CAAGGTGACA AACTGTGACA TAGACACTGA GAAACGCCTG 
AAGAAGGACC TGAAGAGGAC CAAGGCCCTG CTGGCTGATG CCCAGATCAT GTTGGACCAC 
ATAAAGAACA ACGCACCCAG CAAGAGAGAG ATCGCTCAGC TGAAAAACCA GCTGGAGGAG 
TCTGAGTTCA CCTGTGCAGC TGCAGTCAAA GCTCGGAAAT GCATGGAGGT GGAGATAGAA 
GACCTGCATG TTCAGATGGA GGAGATCTCT AAAGCCAAGC AGATGCTGGA AGAACAGCAG 
AGCCGTCTTC AGAGAGAGAA GAACGACCTG CAGTCCAGGA CAGAGGAGGA CCAGGAGGAC 
CTCAACGAGC TGATGAAGAA ACACAAGGCT GCTGTGTCCC AGTTGGCTCA GAACTTGGCT 
CAAATGAACG ACTTGCAGTC TCAGCTGGAG GAGGCCCTCA AGGAGAAACA GGAGGTTCAG 
GAAAAGATGC AGGTCCTCCA GAGCCAGCTG GAGTTCCAGG AGCAATCCAT GGTTGAGAAA 
TCTCTAGCCA ATCGTCAGGA GGCCAAGATC CGAGAACTGG AGACCAAGAT GGAGTTTGAG 
AAGTCGCAGG TCAAACGACT GGAGAATCTT GTAGCTCGTC TGAAGGAGAA CGTGGAAAAG 
CTGACAGAAG AGCGAGACCA GCGCATCAGC AGCGAGAATC GTGAGAAGGA GCAGAATAAG 
CGACTGCAGA GACAGATCCG AGACATTAAA GAGGAAATGG GGGAACTGGC CAAAAAAGAA 
GCAGAGGCAA GCCACAAGAA ACACGAGCTG GAGATGGACA TTGAGAGCCT AGAGGCTTCA 
AACCAGAGCC TGCAGGCGGA CCTTAAGCTG GCCTTCAAAA GGATAGGTGA CCTGCAGGCG 
GCCATCGAGG ACGAGATGGA GAGTGATGAC AACGAAGACC TTATAAACAG TTTGCAGGAT 
ATGGTGGCAA AGTACCAGAA AAGAAGGAAC CGAATTCAGG GAAACTCAGA CACAGACTCT 
GAAGTGGAGG ACCGTGTGGA TGGAGTGAAG TCCTGGTTGT CCAAAAATGA TGGCACTGTC 
AAGAACCTGT CAGATGAGGA CAGCTCCCCC ATCCGAAGAC GCTCCCACTA CGACAGGTCT 
GATGAGGAAG AGGAAGAAGT GAAAGAGGGC GGTCCGGGGA CAACCAGCTC GTCCCGCTCC 
AGATACAGAT ACAGCAGTCA TTTGAAAGAT GACGAAGATG ATGATGATGA TGAGGAGGAG 
GAGGTGTCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023207137.1
CDS	187..6378
Translation	MFSLIKKDKEKEGSKKDKKEKKERMSAAELRSLEEMSMRRGFFNLKREAKRESKHKVEISNPIPIKVASTNELSLTDIESDEFSNRSSMVLDDQISVASSTDDLKGEGGGQDGHRSSVRDRVAHFGSLAKQNSSQMGQMMKRFSFSQRSKEESPSEGSTPSGQNSAAPSPQVESKVFNMLRKHSQRVQPEAADTKKVFIPELVDKIFPAPLQLPTVVAPSAPETREMELQRRNTGDFGFSLRRTTMLDRSPDGGVYRRVVHFAEPGAGTKDLALGLVPGDRLVEINGRNVENKSRDEIVEMIRQSGDAVRLKVQPILELSELSRCWLRNIEGLCREARHESGEEDLFSPFQTSHVAVKTEEQITAEQAWYGSEKVWLVHKEGFSLATVLKTECGSLPEGKVKIKLEHDGTILDVDEDDIEKANPPTYDRSEDLASLVYLNESSMMHCLRQRYGGNLIHSFAGPNMVVINPLSTPSMYSEKVMHMFKGCRREDTAPHIYAVAQSAYRNLLTTQQDQSIVLLGKSGSGKTTNCQHLVQYLVSIAGSTGKIFSAEKWQAVYTILEAFGNCSTTMNINASRFSHVVSLDFDQAGQVASASIQTMLLDKLRVSRRPEAESTFNVFYYVMAGADSALRTELHLNQLNESSAFGIHSHSKTEDKQRAVQQFTKLQAAMKVLGISVEEQRALWLILGAIYHLGAAGATKDEDEAGRRQFAHHEWAQKAAYLLGCTLDELSSLIFKNQTRGMQPLFRGGPDDAGTGDNSGSKFTALECLESMASGLYSEVFTVVISLINRALKSSQHSLCSILIVDTPGFQNPRLAQQQRGATFEELCHNYTQERLQTLFHERTFVQELERYKEENIELVLDDVESSSSLSIAAIDQAPPQALVRTLARMEEARGLLWLMEEEGVKPGGSEEILLERLFSYYSSGLGENKEASLLLRGEKSHLFLMGHCHGTDWVEYNVQGWLGHAKNNPAAQSAATLLQDSQKKNISGLFLGRSSGATVLSGSIAGLEGSSQLALRRATSMRKTFTTGVAAVKKKSHCIQVKLQVDALIDMVRRSKVHFVHCLLPKAESVGGSDPRVPHGESSDSGLMTLDLGLLRAQLRGSKLLDALRIYRQGYPDHMVFSEFRRRFDVLAPHLTKKHGRTDVVPDEKRAVQELLESLELEKSSYHLGLSKVFFRAGTLSMLEEQRDVQTRRNISLFQAACRGYLARQAFKKRKIQDLAIRCIQKNIKKNRGVKGWPWWKLFTTVRPLIEVQLTEEQIRGKDEEIQQLKQKLEKMEKERNELRLNSDRLESRMTELSTELADERNTAESASQLLEAETSERLRLEKDMKDLQTKFDSMKKQTESMEMEVMEARLIRASELNGEMDDDDTAGGEWRLKYERAIREIEFTKKRLQQEFDDKLEVEHQNKRQLERRISDLQADNEESQRNIQQLKKKNQRLVAELQDTKLHLEGQQSRNHDLEKKQRKFDGELSAAQDEVQRERSLREKLAREKDMQSGEAFSLRQQLEEKDLELRTVNLKLEQVEAELQDLNSQESKDEASLAKLKKQLRDMEAKIKDQEEELDEQAGTIQMLEQAKLRLEMEMERLRQTHFKEIESKDDEVEEIRQLCSKKLKQIEVQLEEEYDEKQKVLKEKRELEAKLLSAQSKVTNCDIDTEKRLKKDLKRTKALLADAQIMLDHIKNNAPSKREIAQLKNQLEESEFTCAAAVKARKCMEVEIEDLHVQMEEISKAKQMLEEQQSRLQREKNDLQSRTEEDQEDLNELMKKHKAAVSQLAQNLAQMNDLQSQLEEALKEKQEVQEKMQVLQSQLEFQEQSMVEKSLANRQEAKIRELETKMEFEKSQVKRLENLVARLKENVEKLTEERDQRISSENREKEQNKRLQRQIRDIKEEMGELAKKEAEASHKKHELEMDIESLEASNQSLQADLKLAFKRIGDLQAAIEDEMESDDNEDLINSLQDMVAKYQKRRNRIQGNSDTDSEVEDRVDGVKSWLSKNDGTVKNLSDEDSSPIRRRSHYDRSDEEEEEVKEGGPGTTSSSRSRYRYSSHLKDDEDDDDDEEEEVS

Target ORF information:

RefSeq Version	XM_023351369.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023351369.1

ORF Insert Sequence:

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGAA 
TCAGGGGAAG AGGATCTGTT TTCTCCTTTC CAGACGTCTC ATGTCGCAGT GAAGACGGAG 
GAGCAGATTA CAGCAGAGCA GGCTTGGTAC GGATCAGAGA AGGTCTGGCT TGTTCACAAA 
GAGGGCTTCT CTCTCGCCAC TGTGCTGAAG ACAGAGTGTG GCTCACTCCC CGAGGGAAAG 
GTGAAGATCA AGCTGGAGCA TGATGGAACA ATACTGGATG TGGATGAGGA TGACATAGAA 
AAGGCCAACC CACCAACATA TGACCGCTCA GAGGACCTGG CTTCACTCGT TTACCTAAAT 
GAGTCCAGCA TGATGCACTG CCTGAGGCAG CGCTACGGAG GAAACCTCAT CCACTCTTTT 
GCTGGACCCA ACATGGTCGT TATCAACCCT CTCAGTACAC CTTCTATGTA TTCCGAGAAG 
GTCATGCACA TGTTCAAAGG CTGTCGGCGG GAGGACACTG CCCCTCACAT CTATGCCGTG 
GCCCAGTCAG CCTACCGCAA CCTGCTCACT ACCCAGCAGG ATCAGTCCAT TGTGCTTCTG 
GGGAAGTCTG GCAGCGGTAA AACCACCAAC TGCCAGCATC TGGTCCAGTA CCTGGTCTCT 
ATAGCTGGAA GCACGGGCAA GATCTTCTCT GCTGAGAAGT GGCAGGCAGT CTACACAATC 
CTAGAGGCCT TTGGCAACTG CTCCACAACC ATGAACATAA ACGCCAGCCG CTTCTCTCAT 
GTTGTGTCCC TGGACTTTGA TCAGGCTGGG CAGGTGGCTT CTGCCTCCAT CCAGACCATG 
CTGCTGGACA AACTGAGGGT GAGCAGAAGG CCTGAAGCAG AGTCCACCTT CAATGTGTTT 
TACTATGTGA TGGCTGGAGC TGACAGCGCT CTGAGAACCG AGTTGCACCT TAACCAGCTG 
AATGAAAGCA GTGCTTTTGG GATTCACTCA CACTCCAAGA CTGAAGACAA GCAGCGGGCT 
GTGCAGCAGT TCACCAAGCT GCAGGCGGCC ATGAAGGTGC TGGGCATTTC TGTGGAGGAA 
CAGAGGGCTC TCTGGCTTAT TCTAGGAGCC ATTTATCACT TAGGGGCCGC AGGAGCCACT 
AAAGACGAGG ATGAAGCAGG ACGCAGGCAG TTTGCCCACC ATGAATGGGC TCAGAAGGCA 
GCCTACCTGC TGGGCTGCAC CCTGGATGAG TTGTCCTCTC TGATCTTTAA GAACCAGACC 
CGGGGAATGC AGCCGCTGTT CAGAGGGGGC CCGGATGATG CTGGTACAGG TGACAACTCA 
GGATCTAAAT TCACAGCTCT GGAATGTTTG GAGTCCATGG CATCAGGACT GTACTCAGAG 
GTCTTCACAG TGGTCATCTC TCTCATAAAT AGGGCTCTGA AGTCCAGTCA GCACTCCCTC 
TGTTCCATTC TGATCGTGGA CACGCCGGGT TTCCAGAACC CTCGCCTGGC TCAGCAGCAA 
AGAGGAGCTA CGTTTGAGGA GCTCTGCCAT AACTACACCC AGGAGAGGCT GCAGACTCTG 
TTTCATGAAC GGACCTTTGT CCAGGAATTG GAAAGATATA AGGAGGAAAA CATAGAGCTT 
GTTTTAGATG ACGTAGAGTC CAGTTCCTCC CTGTCTATAG CTGCTATTGA TCAAGCCCCA 
CCACAAGCTC TGGTTCGTAC TCTGGCCCGA ATGGAAGAGG CTCGAGGCCT TTTGTGGCTG 
ATGGAGGAGG AGGGGGTTAA ACCTGGCGGA TCAGAGGAGA TCTTGCTGGA GAGACTCTTT 
AGCTACTACA GTTCTGGTCT GGGAGAAAAC AAAGAAGCCA GTCTGTTACT GCGAGGGGAG 
AAGTCCCACC TCTTCCTTAT GGGCCACTGC CATGGAACGG ATTGGGTGGA GTACAACGTT 
CAGGGCTGGC TGGGCCATGC TAAGAACAAT CCTGCAGCCC AGAGTGCTGC CACTCTGCTC 
CAGGACTCTC AGAAGAAGAA CATCAGCGGT CTGTTCCTGG GCCGGTCCAG CGGGGCCACA 
GTGCTGTCAG GCTCCATTGC CGGCCTGGAG GGCAGCTCTC AGCTAGCTCT GCGCCGCGCC 
ACCAGCATGA GGAAAACCTT CACCACAGGC GTCGCTGCTG TCAAGAAGAA GAGTCACTGC 
ATCCAGGTCA AACTACAAGT GGATGCCTTG ATTGACATGG TGCGCCGTTC CAAGGTCCAC 
TTCGTCCATT GTCTGCTGCC CAAAGCAGAG TCAGTGGGGG GCAGTGACCC CCGTGTTCCT 
CATGGAGAGA GCTCTGATTC TGGGCTGATG ACTTTAGACC TGGGCCTCCT GAGAGCTCAG 
CTCCGGGGCT CCAAGCTGCT AGATGCCCTG CGCATCTACA GGCAAGGTTA CCCAGACCAC 
ATGGTGTTCT CAGAGTTCCG CAGACGCTTC GATGTTCTAG CACCACATCT GACCAAAAAA 
CATGGCCGCA CCGACGTAGT CCCAGATGAG AAGAGAGCAG TGCAGGAGCT GCTGGAGTCC 
CTAGAGCTGG AGAAGAGCAG CTACCACCTG GGGCTAAGCA AGGTGTTTTT CCGAGCTGGG 
ACTTTGTCCA TGCTGGAGGA GCAGCGTGAT GTCCAGACCA GGAGGAACAT CTCTCTGTTC 
CAGGCTGCAT GCAGAGGCTA TCTGGCCAGA CAGGCATTCA AGAAGAGGAA GATCCAGGAT 
CTTGCCATCC GGTGCATCCA GAAGAACATC AAGAAGAACC GCGGTGTGAA GGGCTGGCCA 
TGGTGGAAGC TCTTCACCAC TGTTAGACCT CTGATAGAGG TGCAGCTCAC TGAGGAGCAG 
ATCCGTGGAA AGGATGAGGA GATTCAGCAG CTGAAACAGA AGCTGGAGAA GATGGAGAAG 
GAGAGGAACG AGCTGAGGCT GAACAGTGAC CGACTGGAGA GCAGGATGAC AGAGCTGTCC 
ACAGAGCTAG CTGATGAGCG GAACACGGCC GAGTCGGCCT CCCAGCTGCT GGAGGCAGAG 
ACTTCTGAGA GGCTCCGCTT GGAGAAGGAC ATGAAGGACC TGCAGACAAA GTTTGACAGC 
ATGAAGAAAC AGACGGAGTC CATGGAGATG GAGGTGATGG AGGCTCGGCT CATCAGGGCT 
TCTGAACTCA ATGGAGAGAT GGATGACGAC GACACAGCAG GAGGAGAGTG GCGGTTAAAG 
TATGAGCGAG CCATCAGGGA GATCGAGTTC ACCAAGAAAA GACTGCAGCA GGAGTTTGAT 
GACAAGCTGG AGGTTGAACA TCAGAACAAG CGTCAGCTGG AGAGAAGGAT CAGTGACCTG 
CAGGCTGATA ACGAAGAGTC TCAGCGAAAC ATTCAGCAGT TAAAGAAAAA GAATCAGCGG 
CTAGTAGCTG AGCTGCAGGA CACCAAGCTT CACCTGGAGG GCCAGCAGAG CCGCAACCAT 
GACCTGGAGA AGAAGCAGAG GAAGTTTGAC GGTGAGCTGA GTGCAGCTCA GGACGAAGTG 
CAGAGGGAGA GGAGTCTGAG GGAGAAGTTG GCCCGGGAGA AGGACATGCA GAGTGGGGAG 
GCCTTCAGCC TCAGACAGCA GCTAGAGGAG AAGGACCTTG AATTGCGTAC AGTGAATTTG 
AAGCTGGAGC AGGTTGAAGC AGAGCTACAG GATCTTAACT CCCAGGAATC CAAGGATGAA 
GCATCTCTTG CTAAGCTGAA GAAGCAGCTT CGGGACATGG AAGCCAAGAT CAAGGACCAG 
GAGGAGGAGC TGGATGAGCA AGCTGGCACC ATCCAGATGC TGGAGCAGGC CAAGCTGCGA 
CTGGAGATGG AGATGGAACG TTTGCGTCAG ACACATTTTA AGGAGATCGA AAGCAAGGAT 
GATGAGGTGG AGGAGATCAG GCAGTTATGC AGTAAGAAGT TGAAGCAAAT AGAGGTCCAG 
CTTGAAGAAG AGTATGATGA AAAGCAGAAG GTGCTGAAGG AGAAGAGAGA GCTGGAGGCA 
AAGCTTCTGT CAGCACAGAG CAAGGTGACA AACTGTGACA TAGACACTGA GAAACGCCTG 
AAGAAGGACC TGAAGAGGAC CAAGGCCCTG CTGGCTGATG CCCAGATCAT GTTGGACCAC 
ATAAAGAACA ACGCACCCAG CAAGAGAGAG ATCGCTCAGC TGAAAAACCA GCTGGAGGAG 
TCTGAGTTCA CCTGTGCAGC TGCAGTCAAA GCTCGGAAAT GCATGGAGGT GGAGATAGAA 
GACCTGCATG TTCAGATGGA GGAGATCTCT AAAGCCAAGC AGATGCTGGA AGAACAGCAG 
AGCCGTCTTC AGAGAGAGAA GAACGACCTG CAGTCCAGGA CAGAGGAGGA CCAGGAGGAC 
CTCAACGAGC TGATGAAGAA ACACAAGGCT GCTGTGTCCC AGTTGGCTCA GAACTTGGCT 
CAAATGAACG ACTTGCAGTC TCAGCTGGAG GAGGCCCTCA AGGAGAAACA GGAGGTTCAG 
GAAAAGATGC AGGTCCTCCA GAGCCAGCTG GAGTTCCAGG AGCAATCCAT GGTTGAGAAA 
TCTCTAGCCA ATCGTCAGGA GGCCAAGATC CGAGAACTGG AGACCAAGAT GGAGTTTGAG 
AAGTCGCAGG TCAAACGACT GGAGAATCTT GTAGCTCGTC TGAAGGAGAA CGTGGAAAAG 
CTGACAGAAG AGCGAGACCA GCGCATCAGC AGCGAGAATC GTGAGAAGGA GCAGAATAAG 
CGACTGCAGA GACAGATCCG AGACATTAAA GAGGAAATGG GGGAACTGGC CAAAAAAGAA 
GCAGAGGCAA GCCACAAGAA ACACGAGCTG GAGATGGACA TTGAGAGCCT AGAGGCTTCA 
AACCAGAGCC TGCAGGCGGA CCTTAAGCTG GCCTTCAAAA GGATAGGTGA CCTGCAGGCG 
GCCATCGAGG ACGAGATGGA GAGTGATGAC AACGAAGACC TTATAAACAG TTTGCAGGAT 
ATGGTGGCAA AGTACCAGAA AAGAAGGAAC CGAATTCAGG GAAACTCAGA CACAGACTCT 
GAAGTGGAGG ACCGTGTGGA TGGAGTGAAG TCCTGGTTGT CCAAAAATGA TGGCACTGTC 
AAGAACCTGT CAGATGAGGA CAGCTCCCCC ATCCGAAGAC GCTCCCACTA CGACAGGTCT 
GATGAGGAAG AGGAAGAAGT GAAAGAGGGC GGTCCGGGGA CAACCAGCTC GTCCCGCTCC 
AGATACAGAT ACAGCAGTCA TTTGAAAGAT GACGAAGATG ATGATGATGA TGAGGAGGAG 
GAGGTGTCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo18a ( XM_023351370.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023207138.1 Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X4, mRNA.

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CloneID	OXi28032
Clone ID Related Accession (Same CDS sequence)	XM_023351370.1
Accession Version	XM_023351370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	unconventional myosin-XVIIIa isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036460.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGAA 
TCAGGGGAAG AGGATCTGTT TTCTCCTTTC CAGACGTCTC ATGTCGCAGT GAAGACGGAG 
GAGCAGATTA CAGCAGAGCA GGCTTGGTAC GGATCAGAGA AGGTCTGGCT TGTTCACAAA 
GAGGGCTTCT CTCTCGCCAC TGTGCTGAAG ACAGAGTGTG GCTCACTCCC CGAGGGAAAG 
GTGAAGATCA AGCTGGAGCA TGATGGAACA ATACTGGATG TGGATGAGGA TGACATAGAA 
AAGGCCAACC CACCAACATA TGACCGCTCA GAGGACCTGG CTTCACTCGT TTACCTAAAT 
GAGTCCAGCA TGATGCACTG CCTGAGGCAG CGCTACGGAG GAAACCTCAT CCACTCTTTT 
GCTGGACCCA ACATGGTCGT TATCAACCCT CTCAGTACAC CTTCTATGTA TTCCGAGAAG 
GTCATGCACA TGTTCAAAGG CTGTCGGCGG GAGGACACTG CCCCTCACAT CTATGCCGTG 
GCCCAGTCAG CCTACCGCAA CCTGCTCACT ACCCAGCAGG ATCAGTCCAT TGTGCTTCTG 
GGGAAGTCTG GCAGCGGTAA AACCACCAAC TGCCAGCATC TGGTCCAGTA CCTGGTCTCT 
ATAGCTGGAA GCACGGGCAA GATCTTCTCT GCTGAGAAGT GGCAGGCAGT CTACACAATC 
CTAGAGGCCT TTGGCAACTG CTCCACAACC ATGAACATAA ACGCCAGCCG CTTCTCTCAT 
GTTGTGTCCC TGGACTTTGA TCAGGCTGGG CAGGTGGCTT CTGCCTCCAT CCAGACCATG 
CTGCTGGACA AACTGAGGGT GAGCAGAAGG CCTGAAGCAG AGTCCACCTT CAATGTGTTT 
TACTATGTGA TGGCTGGAGC TGACAGCGCT CTGAGAACCG AGTTGCACCT TAACCAGCTG 
AATGAAAGCA GTGCTTTTGG GATTCACTCA CACTCCAAGA CTGAAGACAA GCAGCGGGCT 
GTGCAGCAGT TCACCAAGCT GCAGGCGGCC ATGAAGGTGC TGGGCATTTC TGTGGAGGAA 
CAGAGGGCTC TCTGGCTTAT TCTAGGAGCC ATTTATCACT TAGGGGCCGC AGGAGCCACT 
AAAGACGAGG ATGAAGCAGG ACGCAGGCAG TTTGCCCACC ATGAATGGGC TCAGAAGGCA 
GCCTACCTGC TGGGCTGCAC CCTGGATGAG TTGTCCTCTC TGATCTTTAA GAACCAGACC 
CGGGGAATGC AGCCGCTGTT CAGAGGGGGC CCGGATGATG CTGGTACAGG TGACAACTCA 
GGATCTAAAT TCACAGCTCT GGAATGTTTG GAGTCCATGG CATCAGGACT GTACTCAGAG 
GTCTTCACAG TGGTCATCTC TCTCATAAAT AGGGCTCTGA AGTCCAGTCA GCACTCCCTC 
TGTTCCATTC TGATCGTGGA CACGCCGGGT TTCCAGAACC CTCGCCTGGC TCAGCAGCAA 
AGAGGAGCTA CGTTTGAGGA GCTCTGCCAT AACTACACCC AGGAGAGGCT GCAGACTCTG 
TTTCATGAAC GGACCTTTGT CCAGGAATTG GAAAGATATA AGGAGGAAAA CATAGAGCTT 
GTTTTAGATG ACGTAGAGTC CAGTTCCTCC CTGTCTATAG CTGCTATTGA TCAAGCCCCA 
CCACAAGCTC TGGTTCGTAC TCTGGCCCGA ATGGAAGAGG CTCGAGGCCT TTTGTGGCTG 
ATGGAGGAGG AGGGGGTTAA ACCTGGCGGA TCAGAGGAGA TCTTGCTGGA GAGACTCTTT 
AGCTACTACA GTTCTGGTCT GGGAGAAAAC AAAGAAGCCA GTCTGTTACT GCGAGGGGAG 
AAGTCCCACC TCTTCCTTAT GGGCCACTGC CATGGAACGG ATTGGGTGGA GTACAACGTT 
CAGGGCTGGC TGGGCCATGC TAAGAACAAT CCTGCAGCCC AGAGTGCTGC CACTCTGCTC 
CAGGACTCTC AGAAGAAGAA CATCAGCGGT CTGTTCCTGG GCCGGTCCAG CGGGGCCACA 
GTGCTGTCAG GCTCCATTGC CGGCCTGGAG GGCAGCTCTC AGCTAGCTCT GCGCCGCGCC 
ACCAGCATGA GGAAAACCTT CACCACAGGC GTCGCTGCTG TCAAGAAGAA GAGTCACTGC 
ATCCAGGTCA AACTACAAGT GGATGCCTTG ATTGACATGG TGCGCCGTTC CAAGGTCCAC 
TTCGTCCATT GTCTGCTGCC CAAAGCAGAG TCAGTGGGGG GCAGTGACCC CCGTGTTCCT 
CATGGAGAGA GCTCTGATTC TGGGCTGATG ACTTTAGACC TGGGCCTCCT GAGAGCTCAG 
CTCCGGGGCT CCAAGCTGCT AGATGCCCTG CGCATCTACA GGCAAGGTTA CCCAGACCAC 
ATGGTGTTCT CAGAGTTCCG CAGACGCTTC GATGTTCTAG CACCACATCT GACCAAAAAA 
CATGGCCGCA CCGACGTAGT CCCAGATGAG AAGAGAGCAG TGCAGGAGCT GCTGGAGTCC 
CTAGAGCTGG AGAAGAGCAG CTACCACCTG GGGCTAAGCA AGGTGTTTTT CCGAGCTGGG 
ACTTTGTCCA TGCTGGAGGA GCAGCGTGAT GTCCAGACCA GGAGGAACAT CTCTCTGTTC 
CAGGCTGCAT GCAGAGGCTA TCTGGCCAGA CAGGCATTCA AGAAGAGGAA GATCCAGGAT 
CTTGCCATCC GGTGCATCCA GAAGAACATC AAGAAGAACC GCGGTGTGAA GGGCTGGCCA 
TGGTGGAAGC TCTTCACCAC TGTTAGACCT CTGATAGAGG TGCAGCTCAC TGAGGAGCAG 
ATCCGTGGAA AGGATGAGGA GATTCAGCAG CTGAAACAGA AGCTGGAGAA GATGGAGAAG 
GAGAGGAACG AGCTGAGGCT GAACAGTGAC CGACTGGAGA GCAGGATGAC AGAGCTGTCC 
ACAGAGCTAG CTGATGAGCG GAACACGGCC GAGTCGGCCT CCCAGCTGCT GGAGGCAGAG 
ACTTCTGAGA GGCTCCGCTT GGAGAAGGAC ATGAAGGACC TGCAGACAAA GTTTGACAGC 
ATGAAGAAAC AGACGGAGTC CATGGAGATG GAGGTGATGG AGGCTCGGCT CATCAGGGCT 
TCTGAACTCA ATGGAGAGAT GGATGACGAC GACACAGCAG GAGGAGAGTG GCGGTTAAAG 
TATGAGCGAG CCATCAGGGA GATCGAGTTC ACCAAGAAAA GACTGCAGCA GGAGTTTGAT 
GACAAGCTGG AGGTTGAACA TCAGAACAAG CGTCAGCTGG AGAGAAGGAT CAGTGACCTG 
CAGGCTGATA ACGAAGAGTC TCAGCGAAAC ATTCAGCAGT TAAAGAAAAA GAATCAGCGG 
CTAGTAGCTG AGCTGCAGGA CACCAAGCTT CACCTGGAGG GCCAGCAGAG CCGCAACCAT 
GACCTGGAGA AGAAGCAGAG GAAGTTTGAC GGTGAGCTGA GTGCAGCTCA GGACGAAGTG 
CAGAGGGAGA GGAGTCTGAG GGAGAAGTTG GCCCGGGAGA AGGACATGCA GAGTGGGGAG 
GCCTTCAGCC TCAGACAGCA GCTAGAGGAG AAGGACCTTG AATTGCGTAC AGTGAATTTG 
AAGCTGGAGC AGGTTGAAGC AGAGCTACAG GATCTTAACT CCCAGGAATC CAAGGATGAA 
GCATCTCTTG CTAAGCTGAA GAAGCAGCTT CGGGACATGG AAGCCAAGAT CAAGGACCAG 
GAGGAGGAGC TGGATGAGCA AGCTGGCACC ATCCAGATGC TGGAGCAGGC CAAGCTGCGA 
CTGGAGATGG AGATGGAACG TTTGCGTCAG ACACATTTTA AGGAGATCGA AAGCAAGGAT 
GATGAGGTGG AGGAGATCAG GCAGTTATGC AGTAAGAAGT TGAAGCAAAT AGAGGTCCAG 
CTTGAAGAAG AGTATGATGA AAAGCAGAAG GTGCTGAAGG AGAAGAGAGA GCTGGAGGCA 
AAGCTTCTGT CAGCACAGAG CAAGGTGACA AACTGTGACA TAGACACTGA GAAACGCCTG 
AAGAAGGACC TGAAGAGGAC CAAGGCCCTG CTGGCTGATG CCCAGATCAT GTTGGACCAC 
ATAAAGAACA ACGCACCCAG CAAGAGAGAG ATCGCTCAGC TGAAAAACCA GCTGGAGGAG 
TCTGAGTTCA CCTGTGCAGC TGCAGTCAAA GCTCGGAAAT GCATGGAGGT GGAGATAGAA 
GACCTGCATG TTCAGATGGA GGAGATCTCT AAAGCCAAGC AGATGCTGGA AGAACAGCAG 
AGCCGTCTTC AGAGAGAGAA GAACGACCTG CAGTCCAGGA CAGAGGAGGA CCAGGAGGAC 
CTCAACGAGC TGATGAAGAA ACACAAGGCT GCTGTGTCCC AGTTGGCTCA GAACTTGGCT 
CAAATGAACG ACTTGCAGTC TCAGCTGGAG GAGGCCCTCA AGGAGAAACA GGAGGTTCAG 
GAAAAGATGC AGGTCCTCCA GAGCCAGCTG GAGTTCCAGG AGCAATCCAT GGTTGAGAAA 
TCTCTAGCCA ATCGTCAGGA GGCCAAGATC CGAGAACTGG AGACCAAGAT GGAGTTTGAG 
AAGTCGCAGG TCAAACGACT GGAGAATCTT GTAGCTCGTC TGAAGGAGAA CGTGGAAAAG 
CTGACAGAAG AGCGAGACCA GCGCATCAGC AGCGAGAATC GTGAGAAGGA GCAGAATAAG 
CGACTGCAGA GACAGATCCG AGACATTAAA GAGGAAATGG GGGAACTGGC CAAAAAAGAA 
GCAGAGGCAA GCCACAAGAA ACACGAGCTG GAGATGGACA TTGAGAGCCT AGAGGCTTCA 
AACCAGAGCC TGCAGGCGGA CCTTAAGCTG GCCTTCAAAA GGATAGGTGA CCTGCAGGCG 
GCCATCGAGG ACGAGATGGA GAGTGATGAC AACGAAGACC TTATAAACAG TTTGCAGGAT 
ATGGTGGCAA AGTACCAGAA AAGAAGGAAC CGAATCTCCC CCATCCGAAG ACGCTCCCAC 
TACGACAGGT CTGATGAGGA AGAGGAAGAA GTGAAAGAGG GCGGTCCGGG GACAACCAGC 
TCGTCCCGCT CCAGATACAG ATACAGCAGT CATTTGAAAG ATGACGAAGA TGATGATGAT 
GATGAGGAGG AGGAGGTGTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023207138.1
CDS	187..6270
Translation	MFSLIKKDKEKEGSKKDKKEKKERMSAAELRSLEEMSMRRGFFNLKREAKRESKHKVEISNPIPIKVASTNELSLTDIESDEFSNRSSMVLDDQISVASSTDDLKGEGGGQDGHRSSVRDRVAHFGSLAKQNSSQMGQMMKRFSFSQRSKEESPSEGSTPSGQNSAAPSPQVESKVFNMLRKHSQRVQPEAADTKKVFIPELVDKIFPAPLQLPTVVAPSAPETREMELQRRNTGDFGFSLRRTTMLDRSPDGGVYRRVVHFAEPGAGTKDLALGLVPGDRLVEINGRNVENKSRDEIVEMIRQSGDAVRLKVQPILELSELSRCWLRNIEGLCREARHESGEEDLFSPFQTSHVAVKTEEQITAEQAWYGSEKVWLVHKEGFSLATVLKTECGSLPEGKVKIKLEHDGTILDVDEDDIEKANPPTYDRSEDLASLVYLNESSMMHCLRQRYGGNLIHSFAGPNMVVINPLSTPSMYSEKVMHMFKGCRREDTAPHIYAVAQSAYRNLLTTQQDQSIVLLGKSGSGKTTNCQHLVQYLVSIAGSTGKIFSAEKWQAVYTILEAFGNCSTTMNINASRFSHVVSLDFDQAGQVASASIQTMLLDKLRVSRRPEAESTFNVFYYVMAGADSALRTELHLNQLNESSAFGIHSHSKTEDKQRAVQQFTKLQAAMKVLGISVEEQRALWLILGAIYHLGAAGATKDEDEAGRRQFAHHEWAQKAAYLLGCTLDELSSLIFKNQTRGMQPLFRGGPDDAGTGDNSGSKFTALECLESMASGLYSEVFTVVISLINRALKSSQHSLCSILIVDTPGFQNPRLAQQQRGATFEELCHNYTQERLQTLFHERTFVQELERYKEENIELVLDDVESSSSLSIAAIDQAPPQALVRTLARMEEARGLLWLMEEEGVKPGGSEEILLERLFSYYSSGLGENKEASLLLRGEKSHLFLMGHCHGTDWVEYNVQGWLGHAKNNPAAQSAATLLQDSQKKNISGLFLGRSSGATVLSGSIAGLEGSSQLALRRATSMRKTFTTGVAAVKKKSHCIQVKLQVDALIDMVRRSKVHFVHCLLPKAESVGGSDPRVPHGESSDSGLMTLDLGLLRAQLRGSKLLDALRIYRQGYPDHMVFSEFRRRFDVLAPHLTKKHGRTDVVPDEKRAVQELLESLELEKSSYHLGLSKVFFRAGTLSMLEEQRDVQTRRNISLFQAACRGYLARQAFKKRKIQDLAIRCIQKNIKKNRGVKGWPWWKLFTTVRPLIEVQLTEEQIRGKDEEIQQLKQKLEKMEKERNELRLNSDRLESRMTELSTELADERNTAESASQLLEAETSERLRLEKDMKDLQTKFDSMKKQTESMEMEVMEARLIRASELNGEMDDDDTAGGEWRLKYERAIREIEFTKKRLQQEFDDKLEVEHQNKRQLERRISDLQADNEESQRNIQQLKKKNQRLVAELQDTKLHLEGQQSRNHDLEKKQRKFDGELSAAQDEVQRERSLREKLAREKDMQSGEAFSLRQQLEEKDLELRTVNLKLEQVEAELQDLNSQESKDEASLAKLKKQLRDMEAKIKDQEEELDEQAGTIQMLEQAKLRLEMEMERLRQTHFKEIESKDDEVEEIRQLCSKKLKQIEVQLEEEYDEKQKVLKEKRELEAKLLSAQSKVTNCDIDTEKRLKKDLKRTKALLADAQIMLDHIKNNAPSKREIAQLKNQLEESEFTCAAAVKARKCMEVEIEDLHVQMEEISKAKQMLEEQQSRLQREKNDLQSRTEEDQEDLNELMKKHKAAVSQLAQNLAQMNDLQSQLEEALKEKQEVQEKMQVLQSQLEFQEQSMVEKSLANRQEAKIRELETKMEFEKSQVKRLENLVARLKENVEKLTEERDQRISSENREKEQNKRLQRQIRDIKEEMGELAKKEAEASHKKHELEMDIESLEASNQSLQADLKLAFKRIGDLQAAIEDEMESDDNEDLINSLQDMVAKYQKRRNRISPIRRRSHYDRSDEEEEEVKEGGPGTTSSSRSRYRYSSHLKDDEDDDDDEEEEVS

Target ORF information:

RefSeq Version	XM_023351370.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023351370.1

ORF Insert Sequence:

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGAA 
TCAGGGGAAG AGGATCTGTT TTCTCCTTTC CAGACGTCTC ATGTCGCAGT GAAGACGGAG 
GAGCAGATTA CAGCAGAGCA GGCTTGGTAC GGATCAGAGA AGGTCTGGCT TGTTCACAAA 
GAGGGCTTCT CTCTCGCCAC TGTGCTGAAG ACAGAGTGTG GCTCACTCCC CGAGGGAAAG 
GTGAAGATCA AGCTGGAGCA TGATGGAACA ATACTGGATG TGGATGAGGA TGACATAGAA 
AAGGCCAACC CACCAACATA TGACCGCTCA GAGGACCTGG CTTCACTCGT TTACCTAAAT 
GAGTCCAGCA TGATGCACTG CCTGAGGCAG CGCTACGGAG GAAACCTCAT CCACTCTTTT 
GCTGGACCCA ACATGGTCGT TATCAACCCT CTCAGTACAC CTTCTATGTA TTCCGAGAAG 
GTCATGCACA TGTTCAAAGG CTGTCGGCGG GAGGACACTG CCCCTCACAT CTATGCCGTG 
GCCCAGTCAG CCTACCGCAA CCTGCTCACT ACCCAGCAGG ATCAGTCCAT TGTGCTTCTG 
GGGAAGTCTG GCAGCGGTAA AACCACCAAC TGCCAGCATC TGGTCCAGTA CCTGGTCTCT 
ATAGCTGGAA GCACGGGCAA GATCTTCTCT GCTGAGAAGT GGCAGGCAGT CTACACAATC 
CTAGAGGCCT TTGGCAACTG CTCCACAACC ATGAACATAA ACGCCAGCCG CTTCTCTCAT 
GTTGTGTCCC TGGACTTTGA TCAGGCTGGG CAGGTGGCTT CTGCCTCCAT CCAGACCATG 
CTGCTGGACA AACTGAGGGT GAGCAGAAGG CCTGAAGCAG AGTCCACCTT CAATGTGTTT 
TACTATGTGA TGGCTGGAGC TGACAGCGCT CTGAGAACCG AGTTGCACCT TAACCAGCTG 
AATGAAAGCA GTGCTTTTGG GATTCACTCA CACTCCAAGA CTGAAGACAA GCAGCGGGCT 
GTGCAGCAGT TCACCAAGCT GCAGGCGGCC ATGAAGGTGC TGGGCATTTC TGTGGAGGAA 
CAGAGGGCTC TCTGGCTTAT TCTAGGAGCC ATTTATCACT TAGGGGCCGC AGGAGCCACT 
AAAGACGAGG ATGAAGCAGG ACGCAGGCAG TTTGCCCACC ATGAATGGGC TCAGAAGGCA 
GCCTACCTGC TGGGCTGCAC CCTGGATGAG TTGTCCTCTC TGATCTTTAA GAACCAGACC 
CGGGGAATGC AGCCGCTGTT CAGAGGGGGC CCGGATGATG CTGGTACAGG TGACAACTCA 
GGATCTAAAT TCACAGCTCT GGAATGTTTG GAGTCCATGG CATCAGGACT GTACTCAGAG 
GTCTTCACAG TGGTCATCTC TCTCATAAAT AGGGCTCTGA AGTCCAGTCA GCACTCCCTC 
TGTTCCATTC TGATCGTGGA CACGCCGGGT TTCCAGAACC CTCGCCTGGC TCAGCAGCAA 
AGAGGAGCTA CGTTTGAGGA GCTCTGCCAT AACTACACCC AGGAGAGGCT GCAGACTCTG 
TTTCATGAAC GGACCTTTGT CCAGGAATTG GAAAGATATA AGGAGGAAAA CATAGAGCTT 
GTTTTAGATG ACGTAGAGTC CAGTTCCTCC CTGTCTATAG CTGCTATTGA TCAAGCCCCA 
CCACAAGCTC TGGTTCGTAC TCTGGCCCGA ATGGAAGAGG CTCGAGGCCT TTTGTGGCTG 
ATGGAGGAGG AGGGGGTTAA ACCTGGCGGA TCAGAGGAGA TCTTGCTGGA GAGACTCTTT 
AGCTACTACA GTTCTGGTCT GGGAGAAAAC AAAGAAGCCA GTCTGTTACT GCGAGGGGAG 
AAGTCCCACC TCTTCCTTAT GGGCCACTGC CATGGAACGG ATTGGGTGGA GTACAACGTT 
CAGGGCTGGC TGGGCCATGC TAAGAACAAT CCTGCAGCCC AGAGTGCTGC CACTCTGCTC 
CAGGACTCTC AGAAGAAGAA CATCAGCGGT CTGTTCCTGG GCCGGTCCAG CGGGGCCACA 
GTGCTGTCAG GCTCCATTGC CGGCCTGGAG GGCAGCTCTC AGCTAGCTCT GCGCCGCGCC 
ACCAGCATGA GGAAAACCTT CACCACAGGC GTCGCTGCTG TCAAGAAGAA GAGTCACTGC 
ATCCAGGTCA AACTACAAGT GGATGCCTTG ATTGACATGG TGCGCCGTTC CAAGGTCCAC 
TTCGTCCATT GTCTGCTGCC CAAAGCAGAG TCAGTGGGGG GCAGTGACCC CCGTGTTCCT 
CATGGAGAGA GCTCTGATTC TGGGCTGATG ACTTTAGACC TGGGCCTCCT GAGAGCTCAG 
CTCCGGGGCT CCAAGCTGCT AGATGCCCTG CGCATCTACA GGCAAGGTTA CCCAGACCAC 
ATGGTGTTCT CAGAGTTCCG CAGACGCTTC GATGTTCTAG CACCACATCT GACCAAAAAA 
CATGGCCGCA CCGACGTAGT CCCAGATGAG AAGAGAGCAG TGCAGGAGCT GCTGGAGTCC 
CTAGAGCTGG AGAAGAGCAG CTACCACCTG GGGCTAAGCA AGGTGTTTTT CCGAGCTGGG 
ACTTTGTCCA TGCTGGAGGA GCAGCGTGAT GTCCAGACCA GGAGGAACAT CTCTCTGTTC 
CAGGCTGCAT GCAGAGGCTA TCTGGCCAGA CAGGCATTCA AGAAGAGGAA GATCCAGGAT 
CTTGCCATCC GGTGCATCCA GAAGAACATC AAGAAGAACC GCGGTGTGAA GGGCTGGCCA 
TGGTGGAAGC TCTTCACCAC TGTTAGACCT CTGATAGAGG TGCAGCTCAC TGAGGAGCAG 
ATCCGTGGAA AGGATGAGGA GATTCAGCAG CTGAAACAGA AGCTGGAGAA GATGGAGAAG 
GAGAGGAACG AGCTGAGGCT GAACAGTGAC CGACTGGAGA GCAGGATGAC AGAGCTGTCC 
ACAGAGCTAG CTGATGAGCG GAACACGGCC GAGTCGGCCT CCCAGCTGCT GGAGGCAGAG 
ACTTCTGAGA GGCTCCGCTT GGAGAAGGAC ATGAAGGACC TGCAGACAAA GTTTGACAGC 
ATGAAGAAAC AGACGGAGTC CATGGAGATG GAGGTGATGG AGGCTCGGCT CATCAGGGCT 
TCTGAACTCA ATGGAGAGAT GGATGACGAC GACACAGCAG GAGGAGAGTG GCGGTTAAAG 
TATGAGCGAG CCATCAGGGA GATCGAGTTC ACCAAGAAAA GACTGCAGCA GGAGTTTGAT 
GACAAGCTGG AGGTTGAACA TCAGAACAAG CGTCAGCTGG AGAGAAGGAT CAGTGACCTG 
CAGGCTGATA ACGAAGAGTC TCAGCGAAAC ATTCAGCAGT TAAAGAAAAA GAATCAGCGG 
CTAGTAGCTG AGCTGCAGGA CACCAAGCTT CACCTGGAGG GCCAGCAGAG CCGCAACCAT 
GACCTGGAGA AGAAGCAGAG GAAGTTTGAC GGTGAGCTGA GTGCAGCTCA GGACGAAGTG 
CAGAGGGAGA GGAGTCTGAG GGAGAAGTTG GCCCGGGAGA AGGACATGCA GAGTGGGGAG 
GCCTTCAGCC TCAGACAGCA GCTAGAGGAG AAGGACCTTG AATTGCGTAC AGTGAATTTG 
AAGCTGGAGC AGGTTGAAGC AGAGCTACAG GATCTTAACT CCCAGGAATC CAAGGATGAA 
GCATCTCTTG CTAAGCTGAA GAAGCAGCTT CGGGACATGG AAGCCAAGAT CAAGGACCAG 
GAGGAGGAGC TGGATGAGCA AGCTGGCACC ATCCAGATGC TGGAGCAGGC CAAGCTGCGA 
CTGGAGATGG AGATGGAACG TTTGCGTCAG ACACATTTTA AGGAGATCGA AAGCAAGGAT 
GATGAGGTGG AGGAGATCAG GCAGTTATGC AGTAAGAAGT TGAAGCAAAT AGAGGTCCAG 
CTTGAAGAAG AGTATGATGA AAAGCAGAAG GTGCTGAAGG AGAAGAGAGA GCTGGAGGCA 
AAGCTTCTGT CAGCACAGAG CAAGGTGACA AACTGTGACA TAGACACTGA GAAACGCCTG 
AAGAAGGACC TGAAGAGGAC CAAGGCCCTG CTGGCTGATG CCCAGATCAT GTTGGACCAC 
ATAAAGAACA ACGCACCCAG CAAGAGAGAG ATCGCTCAGC TGAAAAACCA GCTGGAGGAG 
TCTGAGTTCA CCTGTGCAGC TGCAGTCAAA GCTCGGAAAT GCATGGAGGT GGAGATAGAA 
GACCTGCATG TTCAGATGGA GGAGATCTCT AAAGCCAAGC AGATGCTGGA AGAACAGCAG 
AGCCGTCTTC AGAGAGAGAA GAACGACCTG CAGTCCAGGA CAGAGGAGGA CCAGGAGGAC 
CTCAACGAGC TGATGAAGAA ACACAAGGCT GCTGTGTCCC AGTTGGCTCA GAACTTGGCT 
CAAATGAACG ACTTGCAGTC TCAGCTGGAG GAGGCCCTCA AGGAGAAACA GGAGGTTCAG 
GAAAAGATGC AGGTCCTCCA GAGCCAGCTG GAGTTCCAGG AGCAATCCAT GGTTGAGAAA 
TCTCTAGCCA ATCGTCAGGA GGCCAAGATC CGAGAACTGG AGACCAAGAT GGAGTTTGAG 
AAGTCGCAGG TCAAACGACT GGAGAATCTT GTAGCTCGTC TGAAGGAGAA CGTGGAAAAG 
CTGACAGAAG AGCGAGACCA GCGCATCAGC AGCGAGAATC GTGAGAAGGA GCAGAATAAG 
CGACTGCAGA GACAGATCCG AGACATTAAA GAGGAAATGG GGGAACTGGC CAAAAAAGAA 
GCAGAGGCAA GCCACAAGAA ACACGAGCTG GAGATGGACA TTGAGAGCCT AGAGGCTTCA 
AACCAGAGCC TGCAGGCGGA CCTTAAGCTG GCCTTCAAAA GGATAGGTGA CCTGCAGGCG 
GCCATCGAGG ACGAGATGGA GAGTGATGAC AACGAAGACC TTATAAACAG TTTGCAGGAT 
ATGGTGGCAA AGTACCAGAA AAGAAGGAAC CGAATCTCCC CCATCCGAAG ACGCTCCCAC 
TACGACAGGT CTGATGAGGA AGAGGAAGAA GTGAAAGAGG GCGGTCCGGG GACAACCAGC 
TCGTCCCGCT CCAGATACAG ATACAGCAGT CATTTGAAAG ATGACGAAGA TGATGATGAT 
GATGAGGAGG AGGAGGTGTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo18a ( XM_023351371.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023207139.1 Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X5, mRNA.

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CloneID	OXi28033
Clone ID Related Accession (Same CDS sequence)	XM_023351371.1
Accession Version	XM_023351371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	unconventional myosin-XVIIIa isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036460.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGAA 
TCAGGGGAAG AGGATCTGTT TTCTCCTTTC CAGACGTCTC ATGTCGCAGT GAAGACGGAG 
GAGCAGATTA CAGCAGAGCA GGCTTGGTAC GGATCAGAGA AGGTCTGGCT TGTTCACAAA 
GAGGGCTTCT CTCTCGCCAC TGTGCTGAAG ACAGAGTGTG GCTCACTCCC CGAGGGAAAG 
GTGAAGATCA AGCTGGAGCA TGATGGAACA ATACTGGATG TGGATGAGGA TGACATAGAA 
AAGGCCAACC CACCAACATA TGACCGCTCA GAGGACCTGG CTTCACTCGT TTACCTAAAT 
GAGTCCAGCA TGATGCACTG CCTGAGGCAG CGCTACGGAG GAAACCTCAT CCACTCTTTT 
GCTGGACCCA ACATGGTCGT TATCAACCCT CTCAGTACAC CTTCTATGTA TTCCGAGAAG 
GTCATGCACA TGTTCAAAGG CTGTCGGCGG GAGGACACTG CCCCTCACAT CTATGCCGTG 
GCCCAGTCAG CCTACCGCAA CCTGCTCACT ACCCAGCAGG ATCAGTCCAT TGTGCTTCTG 
GGGAAGTCTG GCAGCGGTAA AACCACCAAC TGCCAGCATC TGGTCCAGTA CCTGGTCTCT 
ATAGCTGGAA GCACGGGCAA GATCTTCTCT GCTGAGAAGT GGCAGGCAGT CTACACAATC 
CTAGAGGCCT TTGGCAACTG CTCCACAACC ATGAACATAA ACGCCAGCCG CTTCTCTCAT 
GTTGTGTCCC TGGACTTTGA TCAGGCTGGG CAGGTGGCTT CTGCCTCCAT CCAGACCATG 
CTGCTGGACA AACTGAGGGT GAGCAGAAGG CCTGAAGCAG AGTCCACCTT CAATGTGTTT 
TACTATGTGA TGGCTGGAGC TGACAGCGCT CTGAGAACCG AGTTGCACCT TAACCAGCTG 
AATGAAAGCA GTGCTTTTGG GATTCACTCA CACTCCAAGA CTGAAGACAA GCAGCGGGCT 
GTGCAGCAGT TCACCAAGCT GCAGGCGGCC ATGAAGGTGC TGGGCATTTC TGTGGAGGAA 
CAGAGGGCTC TCTGGCTTAT TCTAGGAGCC ATTTATCACT TAGGGGCCGC AGGAGCCACT 
AAAGACGAGG ATGAAGCAGG ACGCAGGCAG TTTGCCCACC ATGAATGGGC TCAGAAGGCA 
GCCTACCTGC TGGGCTGCAC CCTGGATGAG TTGTCCTCTC TGATCTTTAA GAACCAGACC 
CGGGGAATGC AGCCGCTGTT CAGAGGGGGC CCGGATGATG CTGGTACAGG TGACAACTCA 
GGATCTAAAT TCACAGCTCT GGAATGTTTG GAGTCCATGG CATCAGGACT GTACTCAGAG 
GTCTTCACAG TGGTCATCTC TCTCATAAAT AGGGCTCTGA AGTCCAGTCA GCACTCCCTC 
TGTTCCATTC TGATCGTGGA CACGCCGGGT TTCCAGAACC CTCGCCTGGC TCAGCAGCAA 
AGAGGAGCTA CGTTTGAGGA GCTCTGCCAT AACTACACCC AGGAGAGGCT GCAGACTCTG 
TTTCATGAAC GGACCTTTGT CCAGGAATTG GAAAGATATA AGGAGGAAAA CATAGAGCTT 
GTTTTAGATG ACGTAGAGTC CAGTTCCTCC CTGTCTATAG CTGCTATTGA TCAAGCCCCA 
CCACAAGCTC TGGTTCGTAC TCTGGCCCGA ATGGAAGAGG CTCGAGGCCT TTTGTGGCTG 
ATGGAGGAGG AGGGGGTTAA ACCTGGCGGA TCAGAGGAGA TCTTGCTGGA GAGACTCTTT 
AGCTACTACA GTTCTGGTCT GGGAGAAAAC AAAGAAGCCA GTCTGTTACT GCGAGGGGAG 
AAGTCCCACC TCTTCCTTAT GGGCCACTGC CATGGAACGG ATTGGGTGGA GTACAACGTT 
CAGGGCTGGC TGGGCCATGC TAAGAACAAT CCTGCAGCCC AGAGTGCTGC CACTCTGCTC 
CAGGACTCTC AGAAGAAGAA CATCAGCGGT CTGTTCCTGG GCCGGTCCAG CGGGGCCACA 
GTGCTGTCAG GCTCCATTGC CGGCCTGGAG GGCAGCTCTC AGCTAGCTCT GCGCCGCGCC 
ACCAGCATGA GGAAAACCTT CACCACAGGC GTCGCTGCTG TCAAGAAGAA GAGTCACTGC 
ATCCAGGTCA AACTACAAGT GGATGCCTTG ATTGACATGG TGCGCCGTTC CAAGGTCCAC 
TTCGTCCATT GTCTGCTGCC CAAAGCAGAG TCAGTGGGGG GCAGTGACCC CCGTGTTCCT 
CATGGAGAGA GCTCTGATTC TGGGCTGATG ACTTTAGACC TGGGCCTCCT GAGAGCTCAG 
CTCCGGGGCT CCAAGCTGCT AGATGCCCTG CGCATCTACA GGCAAGGTTA CCCAGACCAC 
ATGGTGTTCT CAGAGTTCCG CAGACGCTTC GATGTTCTAG CACCACATCT GACCAAAAAA 
CATGGCCGCA CCGACGTAGT CCCAGATGAG AAGAGAGCAG TGCAGGAGCT GCTGGAGTCC 
CTAGAGCTGG AGAAGAGCAG CTACCACCTG GGGCTAAGCA AGGTGTTTTT CCGAGCTGGG 
ACTTTGTCCA TGCTGGAGGA GCAGCGTGAT GTCCAGACCA GGAGGAACAT CTCTCTGTTC 
CAGGCTGCAT GCAGAGGCTA TCTGGCCAGA CAGGCATTCA AGAAGAGGAA GATCCAGGAT 
CTTGCCATCC GGTGCATCCA GAAGAACATC AAGAAGAACC GCGGTGTGAA GGGCTGGCCA 
TGGTGGAAGC TCTTCACCAC TGTTAGACCT CTGATAGAGG TGCAGCTCAC TGAGGAGCAG 
ATCCGTGGAA AGGATGAGGA GATTCAGCAG CTGAAACAGA AGCTGGAGAA GATGGAGAAG 
GAGAGGAACG AGCTGAGGCT GAACAGTGAC CGACTGGAGA GCAGGATGAC AGAGCTGTCC 
ACAGAGCTAG CTGATGAGCG GAACACGGCC GAGTCGGCCT CCCAGCTGCT GGAGGCAGAG 
ACTTCTGAGA GGCTCCGCTT GGAGAAGGAC ATGAAGGACC TGCAGACAAA GTTTGACAGC 
ATGAAGAAAC AGACGGAGTC CATGGAGATG GAGGTGATGG AGGCTCGGCT CATCAGGGCT 
TCTGAACTCA ATGGAGAGAT GGATGACGAC GACACAGCAG GAGGAGAGTG GCGGTTAAAG 
TATGAGCGAG CCATCAGGGA GATCGAGTTC ACCAAGAAAA GACTGCAGCA GGAGTTTGAT 
GACAAGCTGG AGGTTGAACA TCAGAACAAG CGTCAGCTGG AGAGAAGGAT CAGTGACCTG 
CAGGCTGATA ACGAAGAGTC TCAGCGAAAC ATTCAGCAGT TAAAGAAAAA GAATCAGCGG 
CTAGTAGCTG AGCTGCAGGA CACCAAGCTT CACCTGGAGG GCCAGCAGAG CCGCAACCAT 
GACCTGGAGA AGAAGCAGAG GAAGTTTGAC GGTGAGCTGA GTGCAGCTCA GGACGAAGTG 
CAGAGGGAGA GGAGTCTGAG GGAGAAGTTG GCCCGGGAGA AGGACATGCA GAGTGGGGAG 
GCCTTCAGCC TCAGACAGCA GCTAGAGGAG AAGGACCTTG AATTGCGTAC AGTGAATTTG 
AAGCTGGAGC AGGTTGAAGC AGAGCTACAG GATCTTAACT CCCAGGAATC CAAGGATGAA 
GCATCTCTTG CTAAGCTGAA GAAGCAGCTT CGGGACATGG AAGCCAAGAT CAAGGACCAG 
GAGGAGGAGC TGGATGAGCA AGCTGGCACC ATCCAGATGC TGGAGCAGGC CAAGCTGCGA 
CTGGAGATGG AGATGGAACG TTTGCGTCAG ACACATTTTA AGGAGATCGA AAGCAAGGAT 
GATGAGGTGG AGGAGATCAG GCAGTTATGC AGTAAGAAGT TGAAGCAAAT AGAGGTCCAG 
CTTGAAGAAG AGTATGATGA AAAGCAGAAG GTGCTGAAGG AGAAGAGAGA GCTGGAGGCA 
AAGCTTCTGT CAGCACAGAG CAAGGTGACA AACTGTGACA TAGACACTGA GAAACGCCTG 
AAGAAGGACC TGAAGAGGAC CAAGGCCCTG CTGGCTGATG CCCAGATCAT GTTGGACCAC 
ATAAAGAACA ACGCACCCAG CAAGAGAGAG ATCGCTCAGC TGAAAAACCA GCTGGAGGAG 
TCTGAGTTCA CCTGTGCAGC TGCAGTCAAA GCTCGGAAAT GCATGGAGGT GGAGATAGAA 
GACCTGCATG TTCAGATGGA GGAGATCTCT AAAGCCAAGC AGATGCTGGA AGAACAGCAG 
AGCCGTCTTC AGAGAGAGAA GAACGACCTG CAGTCCAGGA CAGAGGAGGA CCAGGAGGAC 
CTCAACGAGC TGATGAAGAA ACACAAGGCT GCTGTGTCCC AGTTGGCTCA GAACTTGGCT 
CAAATGAACG ACTTGCAGTC TCAGCTGGAG GAGGCCCTCA AGGAGAAACA GGAGGTTCAG 
GAAAAGATGC AGGTCCTCCA GAGCCAGCTG GAGTTCCAGG AGCAATCCAT GGTTGAGAAA 
TCTCTAGCCA ATCGTCAGGA GGCCAAGATC CGAGAACTGG AGACCAAGAT GGAGTTTGAG 
AAGTCGCAGG TCAAACGACT GGAGAATCTT GTAGCTCGTC TGAAGGAGAA CGTGGAAAAG 
CTGACAGAAG AGCGAGACCA GCGCATCAGC AGCGAGAATC GTGAGAAGGA GCAGAATAAG 
CGACTGCAGA GACAGATCCG AGACATTAAA GAGGAAATGG GGGAACTGGC CAAAAAAGAA 
GCAGAGGCAA GCCACAAGAA ACACGAGCTG GAGATGGACA TTGAGAGCCT AGAGGCTTCA 
AACCAGAGCC TGCAGGCGGA CCTTAAGCTG GCCTTCAAAA GGATAGGTGA CCTGCAGGCG 
GCCATCGAGG ACGAGATGGA GAGTGATGAC AACGAAGACC TTATAAACAG CTCCCCCATC 
CGAAGACGCT CCCACTACGA CAGGTCTGAT GAGGAAGAGG AAGAAGTGAA AGAGGGCGGT 
CCGGGGACAA CCAGCTCGTC CCGCTCCAGA TACAGATACA GCAGTCATTT GAAAGATGAC 
GAAGATGATG ATGATGATGA GGAGGAGGAG GTGTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023207139.1
CDS	187..6225
Translation	MFSLIKKDKEKEGSKKDKKEKKERMSAAELRSLEEMSMRRGFFNLKREAKRESKHKVEISNPIPIKVASTNELSLTDIESDEFSNRSSMVLDDQISVASSTDDLKGEGGGQDGHRSSVRDRVAHFGSLAKQNSSQMGQMMKRFSFSQRSKEESPSEGSTPSGQNSAAPSPQVESKVFNMLRKHSQRVQPEAADTKKVFIPELVDKIFPAPLQLPTVVAPSAPETREMELQRRNTGDFGFSLRRTTMLDRSPDGGVYRRVVHFAEPGAGTKDLALGLVPGDRLVEINGRNVENKSRDEIVEMIRQSGDAVRLKVQPILELSELSRCWLRNIEGLCREARHESGEEDLFSPFQTSHVAVKTEEQITAEQAWYGSEKVWLVHKEGFSLATVLKTECGSLPEGKVKIKLEHDGTILDVDEDDIEKANPPTYDRSEDLASLVYLNESSMMHCLRQRYGGNLIHSFAGPNMVVINPLSTPSMYSEKVMHMFKGCRREDTAPHIYAVAQSAYRNLLTTQQDQSIVLLGKSGSGKTTNCQHLVQYLVSIAGSTGKIFSAEKWQAVYTILEAFGNCSTTMNINASRFSHVVSLDFDQAGQVASASIQTMLLDKLRVSRRPEAESTFNVFYYVMAGADSALRTELHLNQLNESSAFGIHSHSKTEDKQRAVQQFTKLQAAMKVLGISVEEQRALWLILGAIYHLGAAGATKDEDEAGRRQFAHHEWAQKAAYLLGCTLDELSSLIFKNQTRGMQPLFRGGPDDAGTGDNSGSKFTALECLESMASGLYSEVFTVVISLINRALKSSQHSLCSILIVDTPGFQNPRLAQQQRGATFEELCHNYTQERLQTLFHERTFVQELERYKEENIELVLDDVESSSSLSIAAIDQAPPQALVRTLARMEEARGLLWLMEEEGVKPGGSEEILLERLFSYYSSGLGENKEASLLLRGEKSHLFLMGHCHGTDWVEYNVQGWLGHAKNNPAAQSAATLLQDSQKKNISGLFLGRSSGATVLSGSIAGLEGSSQLALRRATSMRKTFTTGVAAVKKKSHCIQVKLQVDALIDMVRRSKVHFVHCLLPKAESVGGSDPRVPHGESSDSGLMTLDLGLLRAQLRGSKLLDALRIYRQGYPDHMVFSEFRRRFDVLAPHLTKKHGRTDVVPDEKRAVQELLESLELEKSSYHLGLSKVFFRAGTLSMLEEQRDVQTRRNISLFQAACRGYLARQAFKKRKIQDLAIRCIQKNIKKNRGVKGWPWWKLFTTVRPLIEVQLTEEQIRGKDEEIQQLKQKLEKMEKERNELRLNSDRLESRMTELSTELADERNTAESASQLLEAETSERLRLEKDMKDLQTKFDSMKKQTESMEMEVMEARLIRASELNGEMDDDDTAGGEWRLKYERAIREIEFTKKRLQQEFDDKLEVEHQNKRQLERRISDLQADNEESQRNIQQLKKKNQRLVAELQDTKLHLEGQQSRNHDLEKKQRKFDGELSAAQDEVQRERSLREKLAREKDMQSGEAFSLRQQLEEKDLELRTVNLKLEQVEAELQDLNSQESKDEASLAKLKKQLRDMEAKIKDQEEELDEQAGTIQMLEQAKLRLEMEMERLRQTHFKEIESKDDEVEEIRQLCSKKLKQIEVQLEEEYDEKQKVLKEKRELEAKLLSAQSKVTNCDIDTEKRLKKDLKRTKALLADAQIMLDHIKNNAPSKREIAQLKNQLEESEFTCAAAVKARKCMEVEIEDLHVQMEEISKAKQMLEEQQSRLQREKNDLQSRTEEDQEDLNELMKKHKAAVSQLAQNLAQMNDLQSQLEEALKEKQEVQEKMQVLQSQLEFQEQSMVEKSLANRQEAKIRELETKMEFEKSQVKRLENLVARLKENVEKLTEERDQRISSENREKEQNKRLQRQIRDIKEEMGELAKKEAEASHKKHELEMDIESLEASNQSLQADLKLAFKRIGDLQAAIEDEMESDDNEDLINSSPIRRRSHYDRSDEEEEEVKEGGPGTTSSSRSRYRYSSHLKDDEDDDDDEEEEVS

Target ORF information:

RefSeq Version	XM_023351371.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023351371.1

ORF Insert Sequence:

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGAA 
TCAGGGGAAG AGGATCTGTT TTCTCCTTTC CAGACGTCTC ATGTCGCAGT GAAGACGGAG 
GAGCAGATTA CAGCAGAGCA GGCTTGGTAC GGATCAGAGA AGGTCTGGCT TGTTCACAAA 
GAGGGCTTCT CTCTCGCCAC TGTGCTGAAG ACAGAGTGTG GCTCACTCCC CGAGGGAAAG 
GTGAAGATCA AGCTGGAGCA TGATGGAACA ATACTGGATG TGGATGAGGA TGACATAGAA 
AAGGCCAACC CACCAACATA TGACCGCTCA GAGGACCTGG CTTCACTCGT TTACCTAAAT 
GAGTCCAGCA TGATGCACTG CCTGAGGCAG CGCTACGGAG GAAACCTCAT CCACTCTTTT 
GCTGGACCCA ACATGGTCGT TATCAACCCT CTCAGTACAC CTTCTATGTA TTCCGAGAAG 
GTCATGCACA TGTTCAAAGG CTGTCGGCGG GAGGACACTG CCCCTCACAT CTATGCCGTG 
GCCCAGTCAG CCTACCGCAA CCTGCTCACT ACCCAGCAGG ATCAGTCCAT TGTGCTTCTG 
GGGAAGTCTG GCAGCGGTAA AACCACCAAC TGCCAGCATC TGGTCCAGTA CCTGGTCTCT 
ATAGCTGGAA GCACGGGCAA GATCTTCTCT GCTGAGAAGT GGCAGGCAGT CTACACAATC 
CTAGAGGCCT TTGGCAACTG CTCCACAACC ATGAACATAA ACGCCAGCCG CTTCTCTCAT 
GTTGTGTCCC TGGACTTTGA TCAGGCTGGG CAGGTGGCTT CTGCCTCCAT CCAGACCATG 
CTGCTGGACA AACTGAGGGT GAGCAGAAGG CCTGAAGCAG AGTCCACCTT CAATGTGTTT 
TACTATGTGA TGGCTGGAGC TGACAGCGCT CTGAGAACCG AGTTGCACCT TAACCAGCTG 
AATGAAAGCA GTGCTTTTGG GATTCACTCA CACTCCAAGA CTGAAGACAA GCAGCGGGCT 
GTGCAGCAGT TCACCAAGCT GCAGGCGGCC ATGAAGGTGC TGGGCATTTC TGTGGAGGAA 
CAGAGGGCTC TCTGGCTTAT TCTAGGAGCC ATTTATCACT TAGGGGCCGC AGGAGCCACT 
AAAGACGAGG ATGAAGCAGG ACGCAGGCAG TTTGCCCACC ATGAATGGGC TCAGAAGGCA 
GCCTACCTGC TGGGCTGCAC CCTGGATGAG TTGTCCTCTC TGATCTTTAA GAACCAGACC 
CGGGGAATGC AGCCGCTGTT CAGAGGGGGC CCGGATGATG CTGGTACAGG TGACAACTCA 
GGATCTAAAT TCACAGCTCT GGAATGTTTG GAGTCCATGG CATCAGGACT GTACTCAGAG 
GTCTTCACAG TGGTCATCTC TCTCATAAAT AGGGCTCTGA AGTCCAGTCA GCACTCCCTC 
TGTTCCATTC TGATCGTGGA CACGCCGGGT TTCCAGAACC CTCGCCTGGC TCAGCAGCAA 
AGAGGAGCTA CGTTTGAGGA GCTCTGCCAT AACTACACCC AGGAGAGGCT GCAGACTCTG 
TTTCATGAAC GGACCTTTGT CCAGGAATTG GAAAGATATA AGGAGGAAAA CATAGAGCTT 
GTTTTAGATG ACGTAGAGTC CAGTTCCTCC CTGTCTATAG CTGCTATTGA TCAAGCCCCA 
CCACAAGCTC TGGTTCGTAC TCTGGCCCGA ATGGAAGAGG CTCGAGGCCT TTTGTGGCTG 
ATGGAGGAGG AGGGGGTTAA ACCTGGCGGA TCAGAGGAGA TCTTGCTGGA GAGACTCTTT 
AGCTACTACA GTTCTGGTCT GGGAGAAAAC AAAGAAGCCA GTCTGTTACT GCGAGGGGAG 
AAGTCCCACC TCTTCCTTAT GGGCCACTGC CATGGAACGG ATTGGGTGGA GTACAACGTT 
CAGGGCTGGC TGGGCCATGC TAAGAACAAT CCTGCAGCCC AGAGTGCTGC CACTCTGCTC 
CAGGACTCTC AGAAGAAGAA CATCAGCGGT CTGTTCCTGG GCCGGTCCAG CGGGGCCACA 
GTGCTGTCAG GCTCCATTGC CGGCCTGGAG GGCAGCTCTC AGCTAGCTCT GCGCCGCGCC 
ACCAGCATGA GGAAAACCTT CACCACAGGC GTCGCTGCTG TCAAGAAGAA GAGTCACTGC 
ATCCAGGTCA AACTACAAGT GGATGCCTTG ATTGACATGG TGCGCCGTTC CAAGGTCCAC 
TTCGTCCATT GTCTGCTGCC CAAAGCAGAG TCAGTGGGGG GCAGTGACCC CCGTGTTCCT 
CATGGAGAGA GCTCTGATTC TGGGCTGATG ACTTTAGACC TGGGCCTCCT GAGAGCTCAG 
CTCCGGGGCT CCAAGCTGCT AGATGCCCTG CGCATCTACA GGCAAGGTTA CCCAGACCAC 
ATGGTGTTCT CAGAGTTCCG CAGACGCTTC GATGTTCTAG CACCACATCT GACCAAAAAA 
CATGGCCGCA CCGACGTAGT CCCAGATGAG AAGAGAGCAG TGCAGGAGCT GCTGGAGTCC 
CTAGAGCTGG AGAAGAGCAG CTACCACCTG GGGCTAAGCA AGGTGTTTTT CCGAGCTGGG 
ACTTTGTCCA TGCTGGAGGA GCAGCGTGAT GTCCAGACCA GGAGGAACAT CTCTCTGTTC 
CAGGCTGCAT GCAGAGGCTA TCTGGCCAGA CAGGCATTCA AGAAGAGGAA GATCCAGGAT 
CTTGCCATCC GGTGCATCCA GAAGAACATC AAGAAGAACC GCGGTGTGAA GGGCTGGCCA 
TGGTGGAAGC TCTTCACCAC TGTTAGACCT CTGATAGAGG TGCAGCTCAC TGAGGAGCAG 
ATCCGTGGAA AGGATGAGGA GATTCAGCAG CTGAAACAGA AGCTGGAGAA GATGGAGAAG 
GAGAGGAACG AGCTGAGGCT GAACAGTGAC CGACTGGAGA GCAGGATGAC AGAGCTGTCC 
ACAGAGCTAG CTGATGAGCG GAACACGGCC GAGTCGGCCT CCCAGCTGCT GGAGGCAGAG 
ACTTCTGAGA GGCTCCGCTT GGAGAAGGAC ATGAAGGACC TGCAGACAAA GTTTGACAGC 
ATGAAGAAAC AGACGGAGTC CATGGAGATG GAGGTGATGG AGGCTCGGCT CATCAGGGCT 
TCTGAACTCA ATGGAGAGAT GGATGACGAC GACACAGCAG GAGGAGAGTG GCGGTTAAAG 
TATGAGCGAG CCATCAGGGA GATCGAGTTC ACCAAGAAAA GACTGCAGCA GGAGTTTGAT 
GACAAGCTGG AGGTTGAACA TCAGAACAAG CGTCAGCTGG AGAGAAGGAT CAGTGACCTG 
CAGGCTGATA ACGAAGAGTC TCAGCGAAAC ATTCAGCAGT TAAAGAAAAA GAATCAGCGG 
CTAGTAGCTG AGCTGCAGGA CACCAAGCTT CACCTGGAGG GCCAGCAGAG CCGCAACCAT 
GACCTGGAGA AGAAGCAGAG GAAGTTTGAC GGTGAGCTGA GTGCAGCTCA GGACGAAGTG 
CAGAGGGAGA GGAGTCTGAG GGAGAAGTTG GCCCGGGAGA AGGACATGCA GAGTGGGGAG 
GCCTTCAGCC TCAGACAGCA GCTAGAGGAG AAGGACCTTG AATTGCGTAC AGTGAATTTG 
AAGCTGGAGC AGGTTGAAGC AGAGCTACAG GATCTTAACT CCCAGGAATC CAAGGATGAA 
GCATCTCTTG CTAAGCTGAA GAAGCAGCTT CGGGACATGG AAGCCAAGAT CAAGGACCAG 
GAGGAGGAGC TGGATGAGCA AGCTGGCACC ATCCAGATGC TGGAGCAGGC CAAGCTGCGA 
CTGGAGATGG AGATGGAACG TTTGCGTCAG ACACATTTTA AGGAGATCGA AAGCAAGGAT 
GATGAGGTGG AGGAGATCAG GCAGTTATGC AGTAAGAAGT TGAAGCAAAT AGAGGTCCAG 
CTTGAAGAAG AGTATGATGA AAAGCAGAAG GTGCTGAAGG AGAAGAGAGA GCTGGAGGCA 
AAGCTTCTGT CAGCACAGAG CAAGGTGACA AACTGTGACA TAGACACTGA GAAACGCCTG 
AAGAAGGACC TGAAGAGGAC CAAGGCCCTG CTGGCTGATG CCCAGATCAT GTTGGACCAC 
ATAAAGAACA ACGCACCCAG CAAGAGAGAG ATCGCTCAGC TGAAAAACCA GCTGGAGGAG 
TCTGAGTTCA CCTGTGCAGC TGCAGTCAAA GCTCGGAAAT GCATGGAGGT GGAGATAGAA 
GACCTGCATG TTCAGATGGA GGAGATCTCT AAAGCCAAGC AGATGCTGGA AGAACAGCAG 
AGCCGTCTTC AGAGAGAGAA GAACGACCTG CAGTCCAGGA CAGAGGAGGA CCAGGAGGAC 
CTCAACGAGC TGATGAAGAA ACACAAGGCT GCTGTGTCCC AGTTGGCTCA GAACTTGGCT 
CAAATGAACG ACTTGCAGTC TCAGCTGGAG GAGGCCCTCA AGGAGAAACA GGAGGTTCAG 
GAAAAGATGC AGGTCCTCCA GAGCCAGCTG GAGTTCCAGG AGCAATCCAT GGTTGAGAAA 
TCTCTAGCCA ATCGTCAGGA GGCCAAGATC CGAGAACTGG AGACCAAGAT GGAGTTTGAG 
AAGTCGCAGG TCAAACGACT GGAGAATCTT GTAGCTCGTC TGAAGGAGAA CGTGGAAAAG 
CTGACAGAAG AGCGAGACCA GCGCATCAGC AGCGAGAATC GTGAGAAGGA GCAGAATAAG 
CGACTGCAGA GACAGATCCG AGACATTAAA GAGGAAATGG GGGAACTGGC CAAAAAAGAA 
GCAGAGGCAA GCCACAAGAA ACACGAGCTG GAGATGGACA TTGAGAGCCT AGAGGCTTCA 
AACCAGAGCC TGCAGGCGGA CCTTAAGCTG GCCTTCAAAA GGATAGGTGA CCTGCAGGCG 
GCCATCGAGG ACGAGATGGA GAGTGATGAC AACGAAGACC TTATAAACAG CTCCCCCATC 
CGAAGACGCT CCCACTACGA CAGGTCTGAT GAGGAAGAGG AAGAAGTGAA AGAGGGCGGT 
CCGGGGACAA CCAGCTCGTC CCGCTCCAGA TACAGATACA GCAGTCATTT GAAAGATGAC 
GAAGATGATG ATGATGATGA GGAGGAGGAG GTGTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo18a ( XM_023351372.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023207140.1 Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X6, mRNA.

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CloneID	OXi28034
Clone ID Related Accession (Same CDS sequence)	XM_023351372.1
Accession Version	XM_023351372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	unconventional myosin-XVIIIa isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036460.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGTG 
AAGACGGAGG AGCAGATTAC AGCAGAGCAG GCTTGGTACG GATCAGAGAA GGTCTGGCTT 
GTTCACAAAG AGGGCTTCTC TCTCGCCACT GTGCTGAAGA CAGAGTGTGG CTCACTCCCC 
GAGGGAAAGG TGAAGATCAA GCTGGAGCAT GATGGAACAA TACTGGATGT GGATGAGGAT 
GACATAGAAA AGGCCAACCC ACCAACATAT GACCGCTCAG AGGACCTGGC TTCACTCGTT 
TACCTAAATG AGTCCAGCAT GATGCACTGC CTGAGGCAGC GCTACGGAGG AAACCTCATC 
CACTCTTTTG CTGGACCCAA CATGGTCGTT ATCAACCCTC TCAGTACACC TTCTATGTAT 
TCCGAGAAGG TCATGCACAT GTTCAAAGGC TGTCGGCGGG AGGACACTGC CCCTCACATC 
TATGCCGTGG CCCAGTCAGC CTACCGCAAC CTGCTCACTA CCCAGCAGGA TCAGTCCATT 
GTGCTTCTGG GGAAGTCTGG CAGCGGTAAA ACCACCAACT GCCAGCATCT GGTCCAGTAC 
CTGGTCTCTA TAGCTGGAAG CACGGGCAAG ATCTTCTCTG CTGAGAAGTG GCAGGCAGTC 
TACACAATCC TAGAGGCCTT TGGCAACTGC TCCACAACCA TGAACATAAA CGCCAGCCGC 
TTCTCTCATG TTGTGTCCCT GGACTTTGAT CAGGCTGGGC AGGTGGCTTC TGCCTCCATC 
CAGACCATGC TGCTGGACAA ACTGAGGGTG AGCAGAAGGC CTGAAGCAGA GTCCACCTTC 
AATGTGTTTT ACTATGTGAT GGCTGGAGCT GACAGCGCTC TGAGAACCGA GTTGCACCTT 
AACCAGCTGA ATGAAAGCAG TGCTTTTGGG ATTCACTCAC ACTCCAAGAC TGAAGACAAG 
CAGCGGGCTG TGCAGCAGTT CACCAAGCTG CAGGCGGCCA TGAAGGTGCT GGGCATTTCT 
GTGGAGGAAC AGAGGGCTCT CTGGCTTATT CTAGGAGCCA TTTATCACTT AGGGGCCGCA 
GGAGCCACTA AAGACGAGGA TGAAGCAGGA CGCAGGCAGT TTGCCCACCA TGAATGGGCT 
CAGAAGGCAG CCTACCTGCT GGGCTGCACC CTGGATGAGT TGTCCTCTCT GATCTTTAAG 
AACCAGACCC GGGGAATGCA GCCGCTGTTC AGAGGGGGCC CGGATGATGC TGGTACAGGT 
GACAACTCAG GATCTAAATT CACAGCTCTG GAATGTTTGG AGTCCATGGC ATCAGGACTG 
TACTCAGAGG TCTTCACAGT GGTCATCTCT CTCATAAATA GGGCTCTGAA GTCCAGTCAG 
CACTCCCTCT GTTCCATTCT GATCGTGGAC ACGCCGGGTT TCCAGAACCC TCGCCTGGCT 
CAGCAGCAAA GAGGAGCTAC GTTTGAGGAG CTCTGCCATA ACTACACCCA GGAGAGGCTG 
CAGACTCTGT TTCATGAACG GACCTTTGTC CAGGAATTGG AAAGATATAA GGAGGAAAAC 
ATAGAGCTTG TTTTAGATGA CGTAGAGTCC AGTTCCTCCC TGTCTATAGC TGCTATTGAT 
CAAGCCCCAC CACAAGCTCT GGTTCGTACT CTGGCCCGAA TGGAAGAGGC TCGAGGCCTT 
TTGTGGCTGA TGGAGGAGGA GGGGGTTAAA CCTGGCGGAT CAGAGGAGAT CTTGCTGGAG 
AGACTCTTTA GCTACTACAG TTCTGGTCTG GGAGAAAACA AAGAAGCCAG TCTGTTACTG 
CGAGGGGAGA AGTCCCACCT CTTCCTTATG GGCCACTGCC ATGGAACGGA TTGGGTGGAG 
TACAACGTTC AGGGCTGGCT GGGCCATGCT AAGAACAATC CTGCAGCCCA GAGTGCTGCC 
ACTCTGCTCC AGGACTCTCA GAAGAAGAAC ATCAGCGGTC TGTTCCTGGG CCGGTCCAGC 
GGGGCCACAG TGCTGTCAGG CTCCATTGCC GGCCTGGAGG GCAGCTCTCA GCTAGCTCTG 
CGCCGCGCCA CCAGCATGAG GAAAACCTTC ACCACAGGCG TCGCTGCTGT CAAGAAGAAG 
AGTCACTGCA TCCAGGTCAA ACTACAAGTG GATGCCTTGA TTGACATGGT GCGCCGTTCC 
AAGGTCCACT TCGTCCATTG TCTGCTGCCC AAAGCAGAGT CAGTGGGGGG CAGTGACCCC 
CGTGTTCCTC ATGGAGAGAG CTCTGATTCT GGGCTGATGA CTTTAGACCT GGGCCTCCTG 
AGAGCTCAGC TCCGGGGCTC CAAGCTGCTA GATGCCCTGC GCATCTACAG GCAAGGTTAC 
CCAGACCACA TGGTGTTCTC AGAGTTCCGC AGACGCTTCG ATGTTCTAGC ACCACATCTG 
ACCAAAAAAC ATGGCCGCAC CGACGTAGTC CCAGATGAGA AGAGAGCAGT GCAGGAGCTG 
CTGGAGTCCC TAGAGCTGGA GAAGAGCAGC TACCACCTGG GGCTAAGCAA GGTGTTTTTC 
CGAGCTGGGA CTTTGTCCAT GCTGGAGGAG CAGCGTGATG TCCAGACCAG GAGGAACATC 
TCTCTGTTCC AGGCTGCATG CAGAGGCTAT CTGGCCAGAC AGGCATTCAA GAAGAGGAAG 
ATCCAGGATC TTGCCATCCG GTGCATCCAG AAGAACATCA AGAAGAACCG CGGTGTGAAG 
GGCTGGCCAT GGTGGAAGCT CTTCACCACT GTTAGACCTC TGATAGAGGT GCAGCTCACT 
GAGGAGCAGA TCCGTGGAAA GGATGAGGAG ATTCAGCAGC TGAAACAGAA GCTGGAGAAG 
ATGGAGAAGG AGAGGAACGA GCTGAGGCTG AACAGTGACC GACTGGAGAG CAGGATGACA 
GAGCTGTCCA CAGAGCTAGC TGATGAGCGG AACACGGCCG AGTCGGCCTC CCAGCTGCTG 
GAGGCAGAGA CTTCTGAGAG GCTCCGCTTG GAGAAGGACA TGAAGGACCT GCAGACAAAG 
TTTGACAGCA TGAAGAAACA GACGGAGTCC ATGGAGATGG AGGTGATGGA GGCTCGGCTC 
ATCAGGGCTT CTGAACTCAA TGGAGAGATG GATGACGACG ACACAGGAGG AGAGTGGCGG 
TTAAAGTATG AGCGAGCCAT CAGGGAGATC GAGTTCACCA AGAAAAGACT GCAGCAGGAG 
TTTGATGACA AGCTGGAGGT TGAACATCAG AACAAGCGTC AGCTGGAGAG AAGGATCAGT 
GACCTGCAGG CTGATAACGA AGAGTCTCAG CGAAACATTC AGCAGTTAAA GAAAAAGAAT 
CAGCGGCTAG TAGCTGAGCT GCAGGACACC AAGCTTCACC TGGAGGGCCA GCAGAGCCGC 
AACCATGACC TGGAGAAGAA GCAGAGGAAG TTTGACGGTG AGCTGAGTGC AGCTCAGGAC 
GAAGTGCAGA GGGAGAGGAG TCTGAGGGAG AAGTTGGCCC GGGAGAAGGA CATGCAGAGT 
GGGGAGGCCT TCAGCCTCAG ACAGCAGCTA GAGGAGAAGG ACCTTGAATT GCGTACAGTG 
AATTTGAAGC TGGAGCAGGT TGAAGCAGAG CTACAGGATC TTAACTCCCA GGAATCCAAG 
GATGAAGCAT CTCTTGCTAA GCTGAAGAAG CAGCTTCGGG ACATGGAAGC CAAGATCAAG 
GACCAGGAGG AGGAGCTGGA TGAGCAAGCT GGCACCATCC AGATGCTGGA GCAGGCCAAG 
CTGCGACTGG AGATGGAGAT GGAACGTTTG CGTCAGACAC ATTTTAAGGA GATCGAAAGC 
AAGGATGATG AGGTGGAGGA GATCAGGCAG TTATGCAGTA AGAAGTTGAA GCAAATAGAG 
GTCCAGCTTG AAGAAGAGTA TGATGAAAAG CAGAAGGTGC TGAAGGAGAA GAGAGAGCTG 
GAGGCAAAGC TTCTGTCAGC ACAGAGCAAG GTGACAAACT GTGACATAGA CACTGAGAAA 
CGCCTGAAGA AGGACCTGAA GAGGACCAAG GCCCTGCTGG CTGATGCCCA GATCATGTTG 
GACCACATAA AGAACAACGC ACCCAGCAAG AGAGAGATCG CTCAGCTGAA AAACCAGCTG 
GAGGAGTCTG AGTTCACCTG TGCAGCTGCA GTCAAAGCTC GGAAATGCAT GGAGGTGGAG 
ATAGAAGACC TGCATGTTCA GATGGAGGAG ATCTCTAAAG CCAAGCAGAT GCTGGAAGAA 
CAGCAGAGCC GTCTTCAGAG AGAGAAGAAC GACCTGCAGT CCAGGACAGA GGAGGACCAG 
GAGGACCTCA ACGAGCTGAT GAAGAAACAC AAGGCTGCTG TGTCCCAGTT GGCTCAGAAC 
TTGGCTCAAA TGAACGACTT GCAGTCTCAG CTGGAGGAGG CCCTCAAGGA GAAACAGGAG 
GTTCAGGAAA AGATGCAGGT CCTCCAGAGC CAGCTGGAGT TCCAGGAGCA ATCCATGGTT 
GAGAAATCTC TAGCCAATCG TCAGGAGGCC AAGATCCGAG AACTGGAGAC CAAGATGGAG 
TTTGAGAAGT CGCAGGTCAA ACGACTGGAG AATCTTGTAG CTCGTCTGAA GGAGAACGTG 
GAAAAGCTGA CAGAAGAGCG AGACCAGCGC ATCAGCAGCG AGAATCGTGA GAAGGAGCAG 
AATAAGCGAC TGCAGAGACA GATCCGAGAC ATTAAAGAGG AAATGGGGGA ACTGGCCAAA 
AAAGAAGCAG AGGCAAGCCA CAAGAAACAC GAGCTGGAGA TGGACATTGA GAGCCTAGAG 
GCTTCAAACC AGAGCCTGCA GGCGGACCTT AAGCTGGCCT TCAAAAGGAT AGGTGACCTG 
CAGGCGGCCA TCGAGGACGA GATGGAGAGT GATGACAACG AAGACCTTAT AAACAGTTTG 
CAGGATATGG TGGCAAAGTA CCAGAAAAGA AGGAACCGAA TTCAGGGAAA CTCAGACACA 
GACTCTGAAG TGGAGGACCG TGTGGATGGA GTGAAGTCCT GGTTGTCCAA AAATGATGGC 
ACTGTCAAGA ACCTGTCAGA TGAGGACAGA TGTGCTGTAA AAACCGACTT AAAGGAAGGA 
AAAGAGTATA AAGAGGCTAA GGAGAAACAT AAAGAGCAGA GAGAGCGACA GACAATCAGG 
CCGGTGCCTG TTGTGGCCTC TCTTAGTTAT AGAAAAAAAC CTGACCTGGA TTTCATCATA 
GACAAAAGTG ACTCCCTCCT CCGGTCTACC AAGAAAGCAG AGTCAGGCAA CGTCCCAAGC 
AGATGCAAAA CTGAGCTGCT AAACCAGAAA TGTCATGTTG ACCCTTATCT AGCCTCTTCT 
AGGAGACTTC CTCTTTCACT GGATGACGTG AGTGACAAAC AGTCAGTCAT CTCGCAGGCT 
GGTTCAGAGG CTAATGGTAG CATCAGGAAA GGTACGGACA GCCGTTGGGG TCAGTTTAAT 
CTTTCATTCA CTAAGCCAGG ATCATCCTCC ACCCTTCCTG AGATAAACCT GAGGAATAAG 
GTACGTACTG GCAGCAGCTT GGGGCTATCA GGCCTATACA CACACCATCA AAGCATCTCT 
GGTACAGACG GGGCCAGCAG CAGCCAGTGC CCTCCCAGTA GGAGATCAAG AAGTCAAAGT 
CCTGGAAGTG TTGGCCAACT TGATTCCCAG GTATTTCTAG TTGGTAGCAG TTACCTCGGT 
GACGATGGCA TTGACCTAGA TGACAGTAGA AGTGTGGCCT TAACTGAGAG GTCCATGTTC 
AGTCCTCTGT CTTCCATTGG TCGCAGTTTA TCAATGCCTC CACCCAGAGA CAGGCCCACT 
TCTGCTGATC TGCCAATTGA TACCGTGGAC ATTCGGCCGG TCAGCCATCA GAACTACCTT 
GACCCTGACT TGGAGAAAGC CGTCAATGAA GTGCTGAATT ATAAACCAAT AAAATTCAAG 
CGCAGGAGCT TGGAAGACTC TGAAGATGAT GAAAAATCCA GGAGTAATGA AAATGAGAGC 
CGAAAGATGG AGAATGGAGA GAGGATGTAT CCCACCAGAG GTGCTAGATG TTCTGAATCT 
GCTGTGGATT GCAGGAGATC GTCCTGCAGT ACCAGCAGCC ATCACCATGG CAGCAAGAGC 
AAAAGAAAGA GCAAGAAAAA GAGAAGTCAC AGCTCTGAGT CCGACAGTTC TGGAGACAAT 
CTTGTTGAAA GGACCAACTC TAAAAGGTGG CTCAAAAAGT CCCAAAAAAT GTCCAAGAAA 
AAAGAGAAGC GTCCATCATC GTCAGTATCT TCTGAATCAG ATTCTGAGTC AAACTTTACA 
TCAACTTCTG ATGCATCTGC CATTTCCTAC CGGAGTTGCA GTAGTGTGAA AACAGCCGTG 
GGTAGATCGG TTCCCAGTCC AGATGAGGAG GAGACTCTGC CACCCAACAA GAAGCAGCCG 
ATCAGTAAGA AGAATGAGAA ACAAAGGAAG AAGAAGGTGA ACCACCTGAT GATGAAGTAC 
TTGTACCGGA CAGAGAGTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023207140.1
CDS	188..7591
Translation	MFSLIKKDKEKEGSKKDKKEKKERMSAAELRSLEEMSMRRGFFNLKREAKRESKHKVEISNPIPIKVASTNELSLTDIESDEFSNRSSMVLDDQISVASSTDDLKGEGGGQDGHRSSVRDRVAHFGSLAKQNSSQMGQMMKRFSFSQRSKEESPSEGSTPSGQNSAAPSPQVESKVFNMLRKHSQRVQPEAADTKKVFIPELVDKIFPAPLQLPTVVAPSAPETREMELQRRNTGDFGFSLRRTTMLDRSPDGGVYRRVVHFAEPGAGTKDLALGLVPGDRLVEINGRNVENKSRDEIVEMIRQSGDAVRLKVQPILELSELSRCWLRNIEGLCREARHVKTEEQITAEQAWYGSEKVWLVHKEGFSLATVLKTECGSLPEGKVKIKLEHDGTILDVDEDDIEKANPPTYDRSEDLASLVYLNESSMMHCLRQRYGGNLIHSFAGPNMVVINPLSTPSMYSEKVMHMFKGCRREDTAPHIYAVAQSAYRNLLTTQQDQSIVLLGKSGSGKTTNCQHLVQYLVSIAGSTGKIFSAEKWQAVYTILEAFGNCSTTMNINASRFSHVVSLDFDQAGQVASASIQTMLLDKLRVSRRPEAESTFNVFYYVMAGADSALRTELHLNQLNESSAFGIHSHSKTEDKQRAVQQFTKLQAAMKVLGISVEEQRALWLILGAIYHLGAAGATKDEDEAGRRQFAHHEWAQKAAYLLGCTLDELSSLIFKNQTRGMQPLFRGGPDDAGTGDNSGSKFTALECLESMASGLYSEVFTVVISLINRALKSSQHSLCSILIVDTPGFQNPRLAQQQRGATFEELCHNYTQERLQTLFHERTFVQELERYKEENIELVLDDVESSSSLSIAAIDQAPPQALVRTLARMEEARGLLWLMEEEGVKPGGSEEILLERLFSYYSSGLGENKEASLLLRGEKSHLFLMGHCHGTDWVEYNVQGWLGHAKNNPAAQSAATLLQDSQKKNISGLFLGRSSGATVLSGSIAGLEGSSQLALRRATSMRKTFTTGVAAVKKKSHCIQVKLQVDALIDMVRRSKVHFVHCLLPKAESVGGSDPRVPHGESSDSGLMTLDLGLLRAQLRGSKLLDALRIYRQGYPDHMVFSEFRRRFDVLAPHLTKKHGRTDVVPDEKRAVQELLESLELEKSSYHLGLSKVFFRAGTLSMLEEQRDVQTRRNISLFQAACRGYLARQAFKKRKIQDLAIRCIQKNIKKNRGVKGWPWWKLFTTVRPLIEVQLTEEQIRGKDEEIQQLKQKLEKMEKERNELRLNSDRLESRMTELSTELADERNTAESASQLLEAETSERLRLEKDMKDLQTKFDSMKKQTESMEMEVMEARLIRASELNGEMDDDDTGGEWRLKYERAIREIEFTKKRLQQEFDDKLEVEHQNKRQLERRISDLQADNEESQRNIQQLKKKNQRLVAELQDTKLHLEGQQSRNHDLEKKQRKFDGELSAAQDEVQRERSLREKLAREKDMQSGEAFSLRQQLEEKDLELRTVNLKLEQVEAELQDLNSQESKDEASLAKLKKQLRDMEAKIKDQEEELDEQAGTIQMLEQAKLRLEMEMERLRQTHFKEIESKDDEVEEIRQLCSKKLKQIEVQLEEEYDEKQKVLKEKRELEAKLLSAQSKVTNCDIDTEKRLKKDLKRTKALLADAQIMLDHIKNNAPSKREIAQLKNQLEESEFTCAAAVKARKCMEVEIEDLHVQMEEISKAKQMLEEQQSRLQREKNDLQSRTEEDQEDLNELMKKHKAAVSQLAQNLAQMNDLQSQLEEALKEKQEVQEKMQVLQSQLEFQEQSMVEKSLANRQEAKIRELETKMEFEKSQVKRLENLVARLKENVEKLTEERDQRISSENREKEQNKRLQRQIRDIKEEMGELAKKEAEASHKKHELEMDIESLEASNQSLQADLKLAFKRIGDLQAAIEDEMESDDNEDLINSLQDMVAKYQKRRNRIQGNSDTDSEVEDRVDGVKSWLSKNDGTVKNLSDEDRCAVKTDLKEGKEYKEAKEKHKEQRERQTIRPVPVVASLSYRKKPDLDFIIDKSDSLLRSTKKAESGNVPSRCKTELLNQKCHVDPYLASSRRLPLSLDDVSDKQSVISQAGSEANGSIRKGTDSRWGQFNLSFTKPGSSSTLPEINLRNKVRTGSSLGLSGLYTHHQSISGTDGASSSQCPPSRRSRSQSPGSVGQLDSQVFLVGSSYLGDDGIDLDDSRSVALTERSMFSPLSSIGRSLSMPPPRDRPTSADLPIDTVDIRPVSHQNYLDPDLEKAVNEVLNYKPIKFKRRSLEDSEDDEKSRSNENESRKMENGERMYPTRGARCSESAVDCRRSSCSTSSHHHGSKSKRKSKKKRSHSSESDSSGDNLVERTNSKRWLKKSQKMSKKKEKRPSSSVSSESDSESNFTSTSDASAISYRSCSSVKTAVGRSVPSPDEEETLPPNKKQPISKKNEKQRKKKVNHLMMKYLYRTESD

Target ORF information:

RefSeq Version	XM_023351372.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023351372.1

ORF Insert Sequence:

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGTG 
AAGACGGAGG AGCAGATTAC AGCAGAGCAG GCTTGGTACG GATCAGAGAA GGTCTGGCTT 
GTTCACAAAG AGGGCTTCTC TCTCGCCACT GTGCTGAAGA CAGAGTGTGG CTCACTCCCC 
GAGGGAAAGG TGAAGATCAA GCTGGAGCAT GATGGAACAA TACTGGATGT GGATGAGGAT 
GACATAGAAA AGGCCAACCC ACCAACATAT GACCGCTCAG AGGACCTGGC TTCACTCGTT 
TACCTAAATG AGTCCAGCAT GATGCACTGC CTGAGGCAGC GCTACGGAGG AAACCTCATC 
CACTCTTTTG CTGGACCCAA CATGGTCGTT ATCAACCCTC TCAGTACACC TTCTATGTAT 
TCCGAGAAGG TCATGCACAT GTTCAAAGGC TGTCGGCGGG AGGACACTGC CCCTCACATC 
TATGCCGTGG CCCAGTCAGC CTACCGCAAC CTGCTCACTA CCCAGCAGGA TCAGTCCATT 
GTGCTTCTGG GGAAGTCTGG CAGCGGTAAA ACCACCAACT GCCAGCATCT GGTCCAGTAC 
CTGGTCTCTA TAGCTGGAAG CACGGGCAAG ATCTTCTCTG CTGAGAAGTG GCAGGCAGTC 
TACACAATCC TAGAGGCCTT TGGCAACTGC TCCACAACCA TGAACATAAA CGCCAGCCGC 
TTCTCTCATG TTGTGTCCCT GGACTTTGAT CAGGCTGGGC AGGTGGCTTC TGCCTCCATC 
CAGACCATGC TGCTGGACAA ACTGAGGGTG AGCAGAAGGC CTGAAGCAGA GTCCACCTTC 
AATGTGTTTT ACTATGTGAT GGCTGGAGCT GACAGCGCTC TGAGAACCGA GTTGCACCTT 
AACCAGCTGA ATGAAAGCAG TGCTTTTGGG ATTCACTCAC ACTCCAAGAC TGAAGACAAG 
CAGCGGGCTG TGCAGCAGTT CACCAAGCTG CAGGCGGCCA TGAAGGTGCT GGGCATTTCT 
GTGGAGGAAC AGAGGGCTCT CTGGCTTATT CTAGGAGCCA TTTATCACTT AGGGGCCGCA 
GGAGCCACTA AAGACGAGGA TGAAGCAGGA CGCAGGCAGT TTGCCCACCA TGAATGGGCT 
CAGAAGGCAG CCTACCTGCT GGGCTGCACC CTGGATGAGT TGTCCTCTCT GATCTTTAAG 
AACCAGACCC GGGGAATGCA GCCGCTGTTC AGAGGGGGCC CGGATGATGC TGGTACAGGT 
GACAACTCAG GATCTAAATT CACAGCTCTG GAATGTTTGG AGTCCATGGC ATCAGGACTG 
TACTCAGAGG TCTTCACAGT GGTCATCTCT CTCATAAATA GGGCTCTGAA GTCCAGTCAG 
CACTCCCTCT GTTCCATTCT GATCGTGGAC ACGCCGGGTT TCCAGAACCC TCGCCTGGCT 
CAGCAGCAAA GAGGAGCTAC GTTTGAGGAG CTCTGCCATA ACTACACCCA GGAGAGGCTG 
CAGACTCTGT TTCATGAACG GACCTTTGTC CAGGAATTGG AAAGATATAA GGAGGAAAAC 
ATAGAGCTTG TTTTAGATGA CGTAGAGTCC AGTTCCTCCC TGTCTATAGC TGCTATTGAT 
CAAGCCCCAC CACAAGCTCT GGTTCGTACT CTGGCCCGAA TGGAAGAGGC TCGAGGCCTT 
TTGTGGCTGA TGGAGGAGGA GGGGGTTAAA CCTGGCGGAT CAGAGGAGAT CTTGCTGGAG 
AGACTCTTTA GCTACTACAG TTCTGGTCTG GGAGAAAACA AAGAAGCCAG TCTGTTACTG 
CGAGGGGAGA AGTCCCACCT CTTCCTTATG GGCCACTGCC ATGGAACGGA TTGGGTGGAG 
TACAACGTTC AGGGCTGGCT GGGCCATGCT AAGAACAATC CTGCAGCCCA GAGTGCTGCC 
ACTCTGCTCC AGGACTCTCA GAAGAAGAAC ATCAGCGGTC TGTTCCTGGG CCGGTCCAGC 
GGGGCCACAG TGCTGTCAGG CTCCATTGCC GGCCTGGAGG GCAGCTCTCA GCTAGCTCTG 
CGCCGCGCCA CCAGCATGAG GAAAACCTTC ACCACAGGCG TCGCTGCTGT CAAGAAGAAG 
AGTCACTGCA TCCAGGTCAA ACTACAAGTG GATGCCTTGA TTGACATGGT GCGCCGTTCC 
AAGGTCCACT TCGTCCATTG TCTGCTGCCC AAAGCAGAGT CAGTGGGGGG CAGTGACCCC 
CGTGTTCCTC ATGGAGAGAG CTCTGATTCT GGGCTGATGA CTTTAGACCT GGGCCTCCTG 
AGAGCTCAGC TCCGGGGCTC CAAGCTGCTA GATGCCCTGC GCATCTACAG GCAAGGTTAC 
CCAGACCACA TGGTGTTCTC AGAGTTCCGC AGACGCTTCG ATGTTCTAGC ACCACATCTG 
ACCAAAAAAC ATGGCCGCAC CGACGTAGTC CCAGATGAGA AGAGAGCAGT GCAGGAGCTG 
CTGGAGTCCC TAGAGCTGGA GAAGAGCAGC TACCACCTGG GGCTAAGCAA GGTGTTTTTC 
CGAGCTGGGA CTTTGTCCAT GCTGGAGGAG CAGCGTGATG TCCAGACCAG GAGGAACATC 
TCTCTGTTCC AGGCTGCATG CAGAGGCTAT CTGGCCAGAC AGGCATTCAA GAAGAGGAAG 
ATCCAGGATC TTGCCATCCG GTGCATCCAG AAGAACATCA AGAAGAACCG CGGTGTGAAG 
GGCTGGCCAT GGTGGAAGCT CTTCACCACT GTTAGACCTC TGATAGAGGT GCAGCTCACT 
GAGGAGCAGA TCCGTGGAAA GGATGAGGAG ATTCAGCAGC TGAAACAGAA GCTGGAGAAG 
ATGGAGAAGG AGAGGAACGA GCTGAGGCTG AACAGTGACC GACTGGAGAG CAGGATGACA 
GAGCTGTCCA CAGAGCTAGC TGATGAGCGG AACACGGCCG AGTCGGCCTC CCAGCTGCTG 
GAGGCAGAGA CTTCTGAGAG GCTCCGCTTG GAGAAGGACA TGAAGGACCT GCAGACAAAG 
TTTGACAGCA TGAAGAAACA GACGGAGTCC ATGGAGATGG AGGTGATGGA GGCTCGGCTC 
ATCAGGGCTT CTGAACTCAA TGGAGAGATG GATGACGACG ACACAGGAGG AGAGTGGCGG 
TTAAAGTATG AGCGAGCCAT CAGGGAGATC GAGTTCACCA AGAAAAGACT GCAGCAGGAG 
TTTGATGACA AGCTGGAGGT TGAACATCAG AACAAGCGTC AGCTGGAGAG AAGGATCAGT 
GACCTGCAGG CTGATAACGA AGAGTCTCAG CGAAACATTC AGCAGTTAAA GAAAAAGAAT 
CAGCGGCTAG TAGCTGAGCT GCAGGACACC AAGCTTCACC TGGAGGGCCA GCAGAGCCGC 
AACCATGACC TGGAGAAGAA GCAGAGGAAG TTTGACGGTG AGCTGAGTGC AGCTCAGGAC 
GAAGTGCAGA GGGAGAGGAG TCTGAGGGAG AAGTTGGCCC GGGAGAAGGA CATGCAGAGT 
GGGGAGGCCT TCAGCCTCAG ACAGCAGCTA GAGGAGAAGG ACCTTGAATT GCGTACAGTG 
AATTTGAAGC TGGAGCAGGT TGAAGCAGAG CTACAGGATC TTAACTCCCA GGAATCCAAG 
GATGAAGCAT CTCTTGCTAA GCTGAAGAAG CAGCTTCGGG ACATGGAAGC CAAGATCAAG 
GACCAGGAGG AGGAGCTGGA TGAGCAAGCT GGCACCATCC AGATGCTGGA GCAGGCCAAG 
CTGCGACTGG AGATGGAGAT GGAACGTTTG CGTCAGACAC ATTTTAAGGA GATCGAAAGC 
AAGGATGATG AGGTGGAGGA GATCAGGCAG TTATGCAGTA AGAAGTTGAA GCAAATAGAG 
GTCCAGCTTG AAGAAGAGTA TGATGAAAAG CAGAAGGTGC TGAAGGAGAA GAGAGAGCTG 
GAGGCAAAGC TTCTGTCAGC ACAGAGCAAG GTGACAAACT GTGACATAGA CACTGAGAAA 
CGCCTGAAGA AGGACCTGAA GAGGACCAAG GCCCTGCTGG CTGATGCCCA GATCATGTTG 
GACCACATAA AGAACAACGC ACCCAGCAAG AGAGAGATCG CTCAGCTGAA AAACCAGCTG 
GAGGAGTCTG AGTTCACCTG TGCAGCTGCA GTCAAAGCTC GGAAATGCAT GGAGGTGGAG 
ATAGAAGACC TGCATGTTCA GATGGAGGAG ATCTCTAAAG CCAAGCAGAT GCTGGAAGAA 
CAGCAGAGCC GTCTTCAGAG AGAGAAGAAC GACCTGCAGT CCAGGACAGA GGAGGACCAG 
GAGGACCTCA ACGAGCTGAT GAAGAAACAC AAGGCTGCTG TGTCCCAGTT GGCTCAGAAC 
TTGGCTCAAA TGAACGACTT GCAGTCTCAG CTGGAGGAGG CCCTCAAGGA GAAACAGGAG 
GTTCAGGAAA AGATGCAGGT CCTCCAGAGC CAGCTGGAGT TCCAGGAGCA ATCCATGGTT 
GAGAAATCTC TAGCCAATCG TCAGGAGGCC AAGATCCGAG AACTGGAGAC CAAGATGGAG 
TTTGAGAAGT CGCAGGTCAA ACGACTGGAG AATCTTGTAG CTCGTCTGAA GGAGAACGTG 
GAAAAGCTGA CAGAAGAGCG AGACCAGCGC ATCAGCAGCG AGAATCGTGA GAAGGAGCAG 
AATAAGCGAC TGCAGAGACA GATCCGAGAC ATTAAAGAGG AAATGGGGGA ACTGGCCAAA 
AAAGAAGCAG AGGCAAGCCA CAAGAAACAC GAGCTGGAGA TGGACATTGA GAGCCTAGAG 
GCTTCAAACC AGAGCCTGCA GGCGGACCTT AAGCTGGCCT TCAAAAGGAT AGGTGACCTG 
CAGGCGGCCA TCGAGGACGA GATGGAGAGT GATGACAACG AAGACCTTAT AAACAGTTTG 
CAGGATATGG TGGCAAAGTA CCAGAAAAGA AGGAACCGAA TTCAGGGAAA CTCAGACACA 
GACTCTGAAG TGGAGGACCG TGTGGATGGA GTGAAGTCCT GGTTGTCCAA AAATGATGGC 
ACTGTCAAGA ACCTGTCAGA TGAGGACAGA TGTGCTGTAA AAACCGACTT AAAGGAAGGA 
AAAGAGTATA AAGAGGCTAA GGAGAAACAT AAAGAGCAGA GAGAGCGACA GACAATCAGG 
CCGGTGCCTG TTGTGGCCTC TCTTAGTTAT AGAAAAAAAC CTGACCTGGA TTTCATCATA 
GACAAAAGTG ACTCCCTCCT CCGGTCTACC AAGAAAGCAG AGTCAGGCAA CGTCCCAAGC 
AGATGCAAAA CTGAGCTGCT AAACCAGAAA TGTCATGTTG ACCCTTATCT AGCCTCTTCT 
AGGAGACTTC CTCTTTCACT GGATGACGTG AGTGACAAAC AGTCAGTCAT CTCGCAGGCT 
GGTTCAGAGG CTAATGGTAG CATCAGGAAA GGTACGGACA GCCGTTGGGG TCAGTTTAAT 
CTTTCATTCA CTAAGCCAGG ATCATCCTCC ACCCTTCCTG AGATAAACCT GAGGAATAAG 
GTACGTACTG GCAGCAGCTT GGGGCTATCA GGCCTATACA CACACCATCA AAGCATCTCT 
GGTACAGACG GGGCCAGCAG CAGCCAGTGC CCTCCCAGTA GGAGATCAAG AAGTCAAAGT 
CCTGGAAGTG TTGGCCAACT TGATTCCCAG GTATTTCTAG TTGGTAGCAG TTACCTCGGT 
GACGATGGCA TTGACCTAGA TGACAGTAGA AGTGTGGCCT TAACTGAGAG GTCCATGTTC 
AGTCCTCTGT CTTCCATTGG TCGCAGTTTA TCAATGCCTC CACCCAGAGA CAGGCCCACT 
TCTGCTGATC TGCCAATTGA TACCGTGGAC ATTCGGCCGG TCAGCCATCA GAACTACCTT 
GACCCTGACT TGGAGAAAGC CGTCAATGAA GTGCTGAATT ATAAACCAAT AAAATTCAAG 
CGCAGGAGCT TGGAAGACTC TGAAGATGAT GAAAAATCCA GGAGTAATGA AAATGAGAGC 
CGAAAGATGG AGAATGGAGA GAGGATGTAT CCCACCAGAG GTGCTAGATG TTCTGAATCT 
GCTGTGGATT GCAGGAGATC GTCCTGCAGT ACCAGCAGCC ATCACCATGG CAGCAAGAGC 
AAAAGAAAGA GCAAGAAAAA GAGAAGTCAC AGCTCTGAGT CCGACAGTTC TGGAGACAAT 
CTTGTTGAAA GGACCAACTC TAAAAGGTGG CTCAAAAAGT CCCAAAAAAT GTCCAAGAAA 
AAAGAGAAGC GTCCATCATC GTCAGTATCT TCTGAATCAG ATTCTGAGTC AAACTTTACA 
TCAACTTCTG ATGCATCTGC CATTTCCTAC CGGAGTTGCA GTAGTGTGAA AACAGCCGTG 
GGTAGATCGG TTCCCAGTCC AGATGAGGAG GAGACTCTGC CACCCAACAA GAAGCAGCCG 
ATCAGTAAGA AGAATGAGAA ACAAAGGAAG AAGAAGGTGA ACCACCTGAT GATGAAGTAC 
TTGTACCGGA CAGAGAGTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo18a ( XM_023351373.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023207141.1 Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X7, mRNA.

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CloneID	OXi28035
Clone ID Related Accession (Same CDS sequence)	XM_023351373.1
Accession Version	XM_023351373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	unconventional myosin-XVIIIa isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036460.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGTG 
AAGACGGAGG AGCAGATTAC AGCAGAGCAG GCTTGGTACG GATCAGAGAA GGTCTGGCTT 
GTTCACAAAG AGGGCTTCTC TCTCGCCACT GTGCTGAAGA CAGAGTGTGG CTCACTCCCC 
GAGGGAAAGG TGAAGATCAA GCTGGAGCAT GATGGAACAA TACTGGATGT GGATGAGGAT 
GACATAGAAA AGGCCAACCC ACCAACATAT GACCGCTCAG AGGACCTGGC TTCACTCGTT 
TACCTAAATG AGTCCAGCAT GATGCACTGC CTGAGGCAGC GCTACGGAGG AAACCTCATC 
CACTCTTTTG CTGGACCCAA CATGGTCGTT ATCAACCCTC TCAGTACACC TTCTATGTAT 
TCCGAGAAGG TCATGCACAT GTTCAAAGGC TGTCGGCGGG AGGACACTGC CCCTCACATC 
TATGCCGTGG CCCAGTCAGC CTACCGCAAC CTGCTCACTA CCCAGCAGGA TCAGTCCATT 
GTGCTTCTGG GGAAGTCTGG CAGCGGTAAA ACCACCAACT GCCAGCATCT GGTCCAGTAC 
CTGGTCTCTA TAGCTGGAAG CACGGGCAAG ATCTTCTCTG CTGAGAAGTG GCAGGCAGTC 
TACACAATCC TAGAGGCCTT TGGCAACTGC TCCACAACCA TGAACATAAA CGCCAGCCGC 
TTCTCTCATG TTGTGTCCCT GGACTTTGAT CAGGCTGGGC AGGTGGCTTC TGCCTCCATC 
CAGACCATGC TGCTGGACAA ACTGAGGGTG AGCAGAAGGC CTGAAGCAGA GTCCACCTTC 
AATGTGTTTT ACTATGTGAT GGCTGGAGCT GACAGCGCTC TGAGAACCGA GTTGCACCTT 
AACCAGCTGA ATGAAAGCAG TGCTTTTGGG ATTCACTCAC ACTCCAAGAC TGAAGACAAG 
CAGCGGGCTG TGCAGCAGTT CACCAAGCTG CAGGCGGCCA TGAAGGTGCT GGGCATTTCT 
GTGGAGGAAC AGAGGGCTCT CTGGCTTATT CTAGGAGCCA TTTATCACTT AGGGGCCGCA 
GGAGCCACTA AAGACGAGGA TGAAGCAGGA CGCAGGCAGT TTGCCCACCA TGAATGGGCT 
CAGAAGGCAG CCTACCTGCT GGGCTGCACC CTGGATGAGT TGTCCTCTCT GATCTTTAAG 
AACCAGACCC GGGGAATGCA GCCGCTGTTC AGAGGGGGCC CGGATGATGC TGGTACAGGT 
GACAACTCAG GATCTAAATT CACAGCTCTG GAATGTTTGG AGTCCATGGC ATCAGGACTG 
TACTCAGAGG TCTTCACAGT GGTCATCTCT CTCATAAATA GGGCTCTGAA GTCCAGTCAG 
CACTCCCTCT GTTCCATTCT GATCGTGGAC ACGCCGGGTT TCCAGAACCC TCGCCTGGCT 
CAGCAGCAAA GAGGAGCTAC GTTTGAGGAG CTCTGCCATA ACTACACCCA GGAGAGGCTG 
CAGACTCTGT TTCATGAACG GACCTTTGTC CAGGAATTGG AAAGATATAA GGAGGAAAAC 
ATAGAGCTTG TTTTAGATGA CGTAGAGTCC AGTTCCTCCC TGTCTATAGC TGCTATTGAT 
CAAGCCCCAC CACAAGCTCT GGTTCGTACT CTGGCCCGAA TGGAAGAGGC TCGAGGCCTT 
TTGTGGCTGA TGGAGGAGGA GGGGGTTAAA CCTGGCGGAT CAGAGGAGAT CTTGCTGGAG 
AGACTCTTTA GCTACTACAG TTCTGGTCTG GGAGAAAACA AAGAAGCCAG TCTGTTACTG 
CGAGGGGAGA AGTCCCACCT CTTCCTTATG GGCCACTGCC ATGGAACGGA TTGGGTGGAG 
TACAACGTTC AGGGCTGGCT GGGCCATGCT AAGAACAATC CTGCAGCCCA GAGTGCTGCC 
ACTCTGCTCC AGGACTCTCA GAAGAAGAAC ATCAGCGGTC TGTTCCTGGG CCGGTCCAGC 
GGGGCCACAG TGCTGTCAGG CTCCATTGCC GGCCTGGAGG GCAGCTCTCA GCTAGCTCTG 
CGCCGCGCCA CCAGCATGAG GAAAACCTTC ACCACAGGCG TCGCTGCTGT CAAGAAGAAG 
AGTCACTGCA TCCAGGTCAA ACTACAAGTG GATGCCTTGA TTGACATGGT GCGCCGTTCC 
AAGGTCCACT TCGTCCATTG TCTGCTGCCC AAAGCAGAGT CAGTGGGGGG CAGTGACCCC 
CGTGTTCCTC ATGGAGAGAG CTCTGATTCT GGGCTGATGA CTTTAGACCT GGGCCTCCTG 
AGAGCTCAGC TCCGGGGCTC CAAGCTGCTA GATGCCCTGC GCATCTACAG GCAAGGTTAC 
CCAGACCACA TGGTGTTCTC AGAGTTCCGC AGACGCTTCG ATGTTCTAGC ACCACATCTG 
ACCAAAAAAC ATGGCCGCAC CGACGTAGTC CCAGATGAGA AGAGAGCAGT GCAGGAGCTG 
CTGGAGTCCC TAGAGCTGGA GAAGAGCAGC TACCACCTGG GGCTAAGCAA GGTGTTTTTC 
CGAGCTGGGA CTTTGTCCAT GCTGGAGGAG CAGCGTGATG TCCAGACCAG GAGGAACATC 
TCTCTGTTCC AGGCTGCATG CAGAGGCTAT CTGGCCAGAC AGGCATTCAA GAAGAGGAAG 
ATCCAGGATC TTGCCATCCG GTGCATCCAG AAGAACATCA AGAAGAACCG CGGTGTGAAG 
GGCTGGCCAT GGTGGAAGCT CTTCACCACT GTTAGACCTC TGATAGAGGT GCAGCTCACT 
GAGGAGCAGA TCCGTGGAAA GGATGAGGAG ATTCAGCAGC TGAAACAGAA GCTGGAGAAG 
ATGGAGAAGG AGAGGAACGA GCTGAGGCTG AACAGTGACC GACTGGAGAG CAGGATGACA 
GAGCTGTCCA CAGAGCTAGC TGATGAGCGG AACACGGCCG AGTCGGCCTC CCAGCTGCTG 
GAGGCAGAGA CTTCTGAGAG GCTCCGCTTG GAGAAGGACA TGAAGGACCT GCAGACAAAG 
TTTGACAGCA TGAAGAAACA GACGGAGTCC ATGGAGATGG AGGTGATGGA GGCTCGGCTC 
ATCAGGGCTT CTGAACTCAA TGGAGAGATG GATGACGACG ACACAGGAGG AGAGTGGCGG 
TTAAAGTATG AGCGAGCCAT CAGGGAGATC GAGTTCACCA AGAAAAGACT GCAGCAGGAG 
TTTGATGACA AGCTGGAGGT TGAACATCAG AACAAGCGTC AGCTGGAGAG AAGGATCAGT 
GACCTGCAGG CTGATAACGA AGAGTCTCAG CGAAACATTC AGCAGTTAAA GAAAAAGAAT 
CAGCGGCTAG TAGCTGAGCT GCAGGACACC AAGCTTCACC TGGAGGGCCA GCAGAGCCGC 
AACCATGACC TGGAGAAGAA GCAGAGGAAG TTTGACGGTG AGCTGAGTGC AGCTCAGGAC 
GAAGTGCAGA GGGAGAGGAG TCTGAGGGAG AAGTTGGCCC GGGAGAAGGA CATGCAGAGT 
GGGGAGGCCT TCAGCCTCAG ACAGCAGCTA GAGGAGAAGG ACCTTGAATT GCGTACAGTG 
AATTTGAAGC TGGAGCAGGT TGAAGCAGAG CTACAGGATC TTAACTCCCA GGAATCCAAG 
GATGAAGCAT CTCTTGCTAA GCTGAAGAAG CAGCTTCGGG ACATGGAAGC CAAGATCAAG 
GACCAGGAGG AGGAGCTGGA TGAGCAAGCT GGCACCATCC AGATGCTGGA GCAGGCCAAG 
CTGCGACTGG AGATGGAGAT GGAACGTTTG CGTCAGACAC ATTTTAAGGA GATCGAAAGC 
AAGGATGATG AGGTGGAGGA GATCAGGCAG TTATGCAGTA AGAAGTTGAA GCAAATAGAG 
GTCCAGCTTG AAGAAGAGTA TGATGAAAAG CAGAAGGTGC TGAAGGAGAA GAGAGAGCTG 
GAGGCAAAGC TTCTGTCAGC ACAGAGCAAG GTGACAAACT GTGACATAGA CACTGAGAAA 
CGCCTGAAGA AGGACCTGAA GAGGACCAAG GCCCTGCTGG CTGATGCCCA GATCATGTTG 
GACCACATAA AGAACAACGC ACCCAGCAAG AGAGAGATCG CTCAGCTGAA AAACCAGCTG 
GAGGAGTCTG AGTTCACCTG TGCAGCTGCA GTCAAAGCTC GGAAATGCAT GGAGGTGGAG 
ATAGAAGACC TGCATGTTCA GATGGAGGAG ATCTCTAAAG CCAAGCAGAT GCTGGAAGAA 
CAGCAGAGCC GTCTTCAGAG AGAGAAGAAC GACCTGCAGT CCAGGACAGA GGAGGACCAG 
GAGGACCTCA ACGAGCTGAT GAAGAAACAC AAGGCTGCTG TGTCCCAGTT GGCTCAGAAC 
TTGGCTCAAA TGAACGACTT GCAGTCTCAG CTGGAGGAGG CCCTCAAGGA GAAACAGGAG 
GTTCAGGAAA AGATGCAGGT CCTCCAGAGC CAGCTGGAGT TCCAGGAGCA ATCCATGGTT 
GAGAAATCTC TAGCCAATCG TCAGGAGGCC AAGATCCGAG AACTGGAGAC CAAGATGGAG 
TTTGAGAAGT CGCAGGTCAA ACGACTGGAG AATCTTGTAG CTCGTCTGAA GGAGAACGTG 
GAAAAGCTGA CAGAAGAGCG AGACCAGCGC ATCAGCAGCG AGAATCGTGA GAAGGAGCAG 
AATAAGCGAC TGCAGAGACA GATCCGAGAC ATTAAAGAGG AAATGGGGGA ACTGGCCAAA 
AAAGAAGCAG AGGCAAGCCA CAAGAAACAC GAGCTGGAGA TGGACATTGA GAGCCTAGAG 
GCTTCAAACC AGAGCCTGCA GGCGGACCTT AAGCTGGCCT TCAAAAGGAT AGGTGACCTG 
CAGGCGGCCA TCGAGGACGA GATGGAGAGT GATGACAACG AAGACCTTAT AAACAGTCAG 
GGAAACTCAG ACACAGACTC TGAAGTGGAG GACCGTGTGG ATGGAGTGAA GTCCTGGTTG 
TCCAAAAATG ATGGCACTGT CAAGAACCTG TCAGATGAGG ACAGATGTGC TGTAAAAACC 
GACTTAAAGG AAGGAAAAGA GTATAAAGAG GCTAAGGAGA AACATAAAGA GCAGAGAGAG 
CGACAGACAA TCAGGCCGGT GCCTGTTGTG GCCTCTCTTA GTTATAGAAA AAAACCTGAC 
CTGGATTTCA TCATAGACAA AAGTGACTCC CTCCTCCGGT CTACCAAGAA AGCAGAGTCA 
GGCAACGTCC CAAGCAGATG CAAAACTGAG CTGCTAAACC AGAAATGTCA TGTTGACCCT 
TATCTAGCCT CTTCTAGGAG ACTTCCTCTT TCACTGGATG ACGTGAGTGA CAAACAGTCA 
GTCATCTCGC AGGCTGGTTC AGAGGCTAAT GGTAGCATCA GGAAAGGTAC GGACAGCCGT 
TGGGGTCAGT TTAATCTTTC ATTCACTAAG CCAGGATCAT CCTCCACCCT TCCTGAGATA 
AACCTGAGGA ATAAGGTACG TACTGGCAGC AGCTTGGGGC TATCAGGCCT ATACACACAC 
CATCAAAGCA TCTCTGGTAC AGACGGGGCC AGCAGCAGCC AGTGCCCTCC CAGTAGGAGA 
TCAAGAAGTC AAAGTCCTGG AAGTGTTGGC CAACTTGATT CCCAGGTATT TCTAGTTGGT 
AGCAGTTACC TCGGTGACGA TGGCATTGAC CTAGATGACA GTAGAAGTGT GGCCTTAACT 
GAGAGGTCCA TGTTCAGTCC TCTGTCTTCC ATTGGTCGCA GTTTATCAAT GCCTCCACCC 
AGAGACAGGC CCACTTCTGC TGATCTGCCA ATTGATACCG TGGACATTCG GCCGGTCAGC 
CATCAGAACT ACCTTGACCC TGACTTGGAG AAAGCCGTCA ATGAAGTGCT GAATTATAAA 
CCAATAAAAT TCAAGCGCAG GAGCTTGGAA GACTCTGAAG ATGATGAAAA ATCCAGGAGT 
AATGAAAATG AGAGCCGAAA GATGGAGAAT GGAGAGAGGA TGTATCCCAC CAGAGGTGCT 
AGATGTTCTG AATCTGCTGT GGATTGCAGG AGATCGTCCT GCAGTACCAG CAGCCATCAC 
CATGGCAGCA AGAGCAAAAG AAAGAGCAAG AAAAAGAGAA GTCACAGCTC TGAGTCCGAC 
AGTTCTGGAG ACAATCTTGT TGAAAGGACC AACTCTAAAA GGTGGCTCAA AAAGTCCCAA 
AAAATGTCCA AGAAAAAAGA GAAGCGTCCA TCATCGTCAG TATCTTCTGA ATCAGATTCT 
GAGTCAAACT TTACATCAAC TTCTGATGCA TCTGCCATTT CCTACCGGAG TTGCAGTAGT 
GTGAAAACAG CCGTGGGTAG ATCGGTTCCC AGTCCAGATG AGGAGGAGAC TCTGCCACCC 
AACAAGAAGC AGCCGATCAG TAAGAAGAAT GAGAAACAAA GGAAGAAGAA GGTGAACCAC 
CTGATGATGA AGTACTTGTA CCGGACAGAG AGTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023207141.1
CDS	188..7546
Translation	MFSLIKKDKEKEGSKKDKKEKKERMSAAELRSLEEMSMRRGFFNLKREAKRESKHKVEISNPIPIKVASTNELSLTDIESDEFSNRSSMVLDDQISVASSTDDLKGEGGGQDGHRSSVRDRVAHFGSLAKQNSSQMGQMMKRFSFSQRSKEESPSEGSTPSGQNSAAPSPQVESKVFNMLRKHSQRVQPEAADTKKVFIPELVDKIFPAPLQLPTVVAPSAPETREMELQRRNTGDFGFSLRRTTMLDRSPDGGVYRRVVHFAEPGAGTKDLALGLVPGDRLVEINGRNVENKSRDEIVEMIRQSGDAVRLKVQPILELSELSRCWLRNIEGLCREARHVKTEEQITAEQAWYGSEKVWLVHKEGFSLATVLKTECGSLPEGKVKIKLEHDGTILDVDEDDIEKANPPTYDRSEDLASLVYLNESSMMHCLRQRYGGNLIHSFAGPNMVVINPLSTPSMYSEKVMHMFKGCRREDTAPHIYAVAQSAYRNLLTTQQDQSIVLLGKSGSGKTTNCQHLVQYLVSIAGSTGKIFSAEKWQAVYTILEAFGNCSTTMNINASRFSHVVSLDFDQAGQVASASIQTMLLDKLRVSRRPEAESTFNVFYYVMAGADSALRTELHLNQLNESSAFGIHSHSKTEDKQRAVQQFTKLQAAMKVLGISVEEQRALWLILGAIYHLGAAGATKDEDEAGRRQFAHHEWAQKAAYLLGCTLDELSSLIFKNQTRGMQPLFRGGPDDAGTGDNSGSKFTALECLESMASGLYSEVFTVVISLINRALKSSQHSLCSILIVDTPGFQNPRLAQQQRGATFEELCHNYTQERLQTLFHERTFVQELERYKEENIELVLDDVESSSSLSIAAIDQAPPQALVRTLARMEEARGLLWLMEEEGVKPGGSEEILLERLFSYYSSGLGENKEASLLLRGEKSHLFLMGHCHGTDWVEYNVQGWLGHAKNNPAAQSAATLLQDSQKKNISGLFLGRSSGATVLSGSIAGLEGSSQLALRRATSMRKTFTTGVAAVKKKSHCIQVKLQVDALIDMVRRSKVHFVHCLLPKAESVGGSDPRVPHGESSDSGLMTLDLGLLRAQLRGSKLLDALRIYRQGYPDHMVFSEFRRRFDVLAPHLTKKHGRTDVVPDEKRAVQELLESLELEKSSYHLGLSKVFFRAGTLSMLEEQRDVQTRRNISLFQAACRGYLARQAFKKRKIQDLAIRCIQKNIKKNRGVKGWPWWKLFTTVRPLIEVQLTEEQIRGKDEEIQQLKQKLEKMEKERNELRLNSDRLESRMTELSTELADERNTAESASQLLEAETSERLRLEKDMKDLQTKFDSMKKQTESMEMEVMEARLIRASELNGEMDDDDTGGEWRLKYERAIREIEFTKKRLQQEFDDKLEVEHQNKRQLERRISDLQADNEESQRNIQQLKKKNQRLVAELQDTKLHLEGQQSRNHDLEKKQRKFDGELSAAQDEVQRERSLREKLAREKDMQSGEAFSLRQQLEEKDLELRTVNLKLEQVEAELQDLNSQESKDEASLAKLKKQLRDMEAKIKDQEEELDEQAGTIQMLEQAKLRLEMEMERLRQTHFKEIESKDDEVEEIRQLCSKKLKQIEVQLEEEYDEKQKVLKEKRELEAKLLSAQSKVTNCDIDTEKRLKKDLKRTKALLADAQIMLDHIKNNAPSKREIAQLKNQLEESEFTCAAAVKARKCMEVEIEDLHVQMEEISKAKQMLEEQQSRLQREKNDLQSRTEEDQEDLNELMKKHKAAVSQLAQNLAQMNDLQSQLEEALKEKQEVQEKMQVLQSQLEFQEQSMVEKSLANRQEAKIRELETKMEFEKSQVKRLENLVARLKENVEKLTEERDQRISSENREKEQNKRLQRQIRDIKEEMGELAKKEAEASHKKHELEMDIESLEASNQSLQADLKLAFKRIGDLQAAIEDEMESDDNEDLINSQGNSDTDSEVEDRVDGVKSWLSKNDGTVKNLSDEDRCAVKTDLKEGKEYKEAKEKHKEQRERQTIRPVPVVASLSYRKKPDLDFIIDKSDSLLRSTKKAESGNVPSRCKTELLNQKCHVDPYLASSRRLPLSLDDVSDKQSVISQAGSEANGSIRKGTDSRWGQFNLSFTKPGSSSTLPEINLRNKVRTGSSLGLSGLYTHHQSISGTDGASSSQCPPSRRSRSQSPGSVGQLDSQVFLVGSSYLGDDGIDLDDSRSVALTERSMFSPLSSIGRSLSMPPPRDRPTSADLPIDTVDIRPVSHQNYLDPDLEKAVNEVLNYKPIKFKRRSLEDSEDDEKSRSNENESRKMENGERMYPTRGARCSESAVDCRRSSCSTSSHHHGSKSKRKSKKKRSHSSESDSSGDNLVERTNSKRWLKKSQKMSKKKEKRPSSSVSSESDSESNFTSTSDASAISYRSCSSVKTAVGRSVPSPDEEETLPPNKKQPISKKNEKQRKKKVNHLMMKYLYRTESD

Target ORF information:

RefSeq Version	XM_023351373.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus myosin XVIIIA (myo18a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023351373.1

ORF Insert Sequence:

ATGTTTAGTT TGATAAAGAA AGACAAGGAG AAGGAAGGGA GCAAGAAAGA CAAGAAAGAG 
AAGAAGGAGA GAATGTCCGC GGCAGAGCTG CGCAGCCTGG AGGAGATGAG CATGAGGCGG 
GGCTTTTTCA ATCTTAAGAG GGAGGCTAAG CGGGAATCCA AGCACAAAGT GGAGATCTCC 
AACCCCATTC CTATCAAAGT GGCCAGCACC AATGAGCTGT CTCTCACCGA CATTGAGTCG 
GATGAGTTCA GCAACCGGAG CAGCATGGTC TTGGACGATC AGATCAGTGT GGCCAGCTCC 
ACTGATGACT TGAAAGGAGA GGGAGGAGGT CAGGATGGAC ATCGCAGCTC TGTACGGGAC 
CGCGTGGCTC ACTTTGGTTC TCTCGCCAAG CAGAACTCCT CACAGATGGG GCAGATGATG 
AAGCGTTTCT CCTTCTCCCA GAGGAGCAAG GAGGAGAGCC CGTCAGAGGG CTCCACGCCT 
TCTGGACAGA ACTCTGCTGC TCCATCACCG CAGGTCGAGA GCAAAGTGTT CAACATGCTT 
CGCAAGCACA GCCAAAGAGT TCAACCAGAG GCGGCAGACA CCAAAAAAGT TTTCATTCCT 
GAACTGGTTG ATAAGATTTT TCCTGCACCT TTGCAGCTGC CTACTGTGGT TGCACCAAGT 
GCCCCTGAAA CGCGGGAGAT GGAGCTTCAG AGGCGCAACA CTGGTGACTT TGGGTTCTCT 
TTGCGCCGTA CCACTATGCT AGATCGCAGC CCTGATGGAG GGGTCTACAG ACGCGTGGTC 
CACTTCGCCG AGCCTGGTGC TGGCACTAAG GACTTGGCGC TCGGACTTGT GCCTGGCGAC 
AGGCTGGTGG AGATAAACGG ACGGAATGTG GAGAACAAGA GCCGTGATGA GATAGTTGAG 
ATGATCCGTC AGTCAGGAGA CGCAGTGAGG CTGAAGGTCC AGCCCATCCT GGAGCTGAGC 
GAGCTGAGCC GCTGCTGGCT GAGGAACATC GAGGGGCTGT GCAGAGAGGC CCGACACGTG 
AAGACGGAGG AGCAGATTAC AGCAGAGCAG GCTTGGTACG GATCAGAGAA GGTCTGGCTT 
GTTCACAAAG AGGGCTTCTC TCTCGCCACT GTGCTGAAGA CAGAGTGTGG CTCACTCCCC 
GAGGGAAAGG TGAAGATCAA GCTGGAGCAT GATGGAACAA TACTGGATGT GGATGAGGAT 
GACATAGAAA AGGCCAACCC ACCAACATAT GACCGCTCAG AGGACCTGGC TTCACTCGTT 
TACCTAAATG AGTCCAGCAT GATGCACTGC CTGAGGCAGC GCTACGGAGG AAACCTCATC 
CACTCTTTTG CTGGACCCAA CATGGTCGTT ATCAACCCTC TCAGTACACC TTCTATGTAT 
TCCGAGAAGG TCATGCACAT GTTCAAAGGC TGTCGGCGGG AGGACACTGC CCCTCACATC 
TATGCCGTGG CCCAGTCAGC CTACCGCAAC CTGCTCACTA CCCAGCAGGA TCAGTCCATT 
GTGCTTCTGG GGAAGTCTGG CAGCGGTAAA ACCACCAACT GCCAGCATCT GGTCCAGTAC 
CTGGTCTCTA TAGCTGGAAG CACGGGCAAG ATCTTCTCTG CTGAGAAGTG GCAGGCAGTC 
TACACAATCC TAGAGGCCTT TGGCAACTGC TCCACAACCA TGAACATAAA CGCCAGCCGC 
TTCTCTCATG TTGTGTCCCT GGACTTTGAT CAGGCTGGGC AGGTGGCTTC TGCCTCCATC 
CAGACCATGC TGCTGGACAA ACTGAGGGTG AGCAGAAGGC CTGAAGCAGA GTCCACCTTC 
AATGTGTTTT ACTATGTGAT GGCTGGAGCT GACAGCGCTC TGAGAACCGA GTTGCACCTT 
AACCAGCTGA ATGAAAGCAG TGCTTTTGGG ATTCACTCAC ACTCCAAGAC TGAAGACAAG 
CAGCGGGCTG TGCAGCAGTT CACCAAGCTG CAGGCGGCCA TGAAGGTGCT GGGCATTTCT 
GTGGAGGAAC AGAGGGCTCT CTGGCTTATT CTAGGAGCCA TTTATCACTT AGGGGCCGCA 
GGAGCCACTA AAGACGAGGA TGAAGCAGGA CGCAGGCAGT TTGCCCACCA TGAATGGGCT 
CAGAAGGCAG CCTACCTGCT GGGCTGCACC CTGGATGAGT TGTCCTCTCT GATCTTTAAG 
AACCAGACCC GGGGAATGCA GCCGCTGTTC AGAGGGGGCC CGGATGATGC TGGTACAGGT 
GACAACTCAG GATCTAAATT CACAGCTCTG GAATGTTTGG AGTCCATGGC ATCAGGACTG 
TACTCAGAGG TCTTCACAGT GGTCATCTCT CTCATAAATA GGGCTCTGAA GTCCAGTCAG 
CACTCCCTCT GTTCCATTCT GATCGTGGAC ACGCCGGGTT TCCAGAACCC TCGCCTGGCT 
CAGCAGCAAA GAGGAGCTAC GTTTGAGGAG CTCTGCCATA ACTACACCCA GGAGAGGCTG 
CAGACTCTGT TTCATGAACG GACCTTTGTC CAGGAATTGG AAAGATATAA GGAGGAAAAC 
ATAGAGCTTG TTTTAGATGA CGTAGAGTCC AGTTCCTCCC TGTCTATAGC TGCTATTGAT 
CAAGCCCCAC CACAAGCTCT GGTTCGTACT CTGGCCCGAA TGGAAGAGGC TCGAGGCCTT 
TTGTGGCTGA TGGAGGAGGA GGGGGTTAAA CCTGGCGGAT CAGAGGAGAT CTTGCTGGAG 
AGACTCTTTA GCTACTACAG TTCTGGTCTG GGAGAAAACA AAGAAGCCAG TCTGTTACTG 
CGAGGGGAGA AGTCCCACCT CTTCCTTATG GGCCACTGCC ATGGAACGGA TTGGGTGGAG 
TACAACGTTC AGGGCTGGCT GGGCCATGCT AAGAACAATC CTGCAGCCCA GAGTGCTGCC 
ACTCTGCTCC AGGACTCTCA GAAGAAGAAC ATCAGCGGTC TGTTCCTGGG CCGGTCCAGC 
GGGGCCACAG TGCTGTCAGG CTCCATTGCC GGCCTGGAGG GCAGCTCTCA GCTAGCTCTG 
CGCCGCGCCA CCAGCATGAG GAAAACCTTC ACCACAGGCG TCGCTGCTGT CAAGAAGAAG 
AGTCACTGCA TCCAGGTCAA ACTACAAGTG GATGCCTTGA TTGACATGGT GCGCCGTTCC 
AAGGTCCACT TCGTCCATTG TCTGCTGCCC AAAGCAGAGT CAGTGGGGGG CAGTGACCCC 
CGTGTTCCTC ATGGAGAGAG CTCTGATTCT GGGCTGATGA CTTTAGACCT GGGCCTCCTG 
AGAGCTCAGC TCCGGGGCTC CAAGCTGCTA GATGCCCTGC GCATCTACAG GCAAGGTTAC 
CCAGACCACA TGGTGTTCTC AGAGTTCCGC AGACGCTTCG ATGTTCTAGC ACCACATCTG 
ACCAAAAAAC ATGGCCGCAC CGACGTAGTC CCAGATGAGA AGAGAGCAGT GCAGGAGCTG 
CTGGAGTCCC TAGAGCTGGA GAAGAGCAGC TACCACCTGG GGCTAAGCAA GGTGTTTTTC 
CGAGCTGGGA CTTTGTCCAT GCTGGAGGAG CAGCGTGATG TCCAGACCAG GAGGAACATC 
TCTCTGTTCC AGGCTGCATG CAGAGGCTAT CTGGCCAGAC AGGCATTCAA GAAGAGGAAG 
ATCCAGGATC TTGCCATCCG GTGCATCCAG AAGAACATCA AGAAGAACCG CGGTGTGAAG 
GGCTGGCCAT GGTGGAAGCT CTTCACCACT GTTAGACCTC TGATAGAGGT GCAGCTCACT 
GAGGAGCAGA TCCGTGGAAA GGATGAGGAG ATTCAGCAGC TGAAACAGAA GCTGGAGAAG 
ATGGAGAAGG AGAGGAACGA GCTGAGGCTG AACAGTGACC GACTGGAGAG CAGGATGACA 
GAGCTGTCCA CAGAGCTAGC TGATGAGCGG AACACGGCCG AGTCGGCCTC CCAGCTGCTG 
GAGGCAGAGA CTTCTGAGAG GCTCCGCTTG GAGAAGGACA TGAAGGACCT GCAGACAAAG 
TTTGACAGCA TGAAGAAACA GACGGAGTCC ATGGAGATGG AGGTGATGGA GGCTCGGCTC 
ATCAGGGCTT CTGAACTCAA TGGAGAGATG GATGACGACG ACACAGGAGG AGAGTGGCGG 
TTAAAGTATG AGCGAGCCAT CAGGGAGATC GAGTTCACCA AGAAAAGACT GCAGCAGGAG 
TTTGATGACA AGCTGGAGGT TGAACATCAG AACAAGCGTC AGCTGGAGAG AAGGATCAGT 
GACCTGCAGG CTGATAACGA AGAGTCTCAG CGAAACATTC AGCAGTTAAA GAAAAAGAAT 
CAGCGGCTAG TAGCTGAGCT GCAGGACACC AAGCTTCACC TGGAGGGCCA GCAGAGCCGC 
AACCATGACC TGGAGAAGAA GCAGAGGAAG TTTGACGGTG AGCTGAGTGC AGCTCAGGAC 
GAAGTGCAGA GGGAGAGGAG TCTGAGGGAG AAGTTGGCCC GGGAGAAGGA CATGCAGAGT 
GGGGAGGCCT TCAGCCTCAG ACAGCAGCTA GAGGAGAAGG ACCTTGAATT GCGTACAGTG 
AATTTGAAGC TGGAGCAGGT TGAAGCAGAG CTACAGGATC TTAACTCCCA GGAATCCAAG 
GATGAAGCAT CTCTTGCTAA GCTGAAGAAG CAGCTTCGGG ACATGGAAGC CAAGATCAAG 
GACCAGGAGG AGGAGCTGGA TGAGCAAGCT GGCACCATCC AGATGCTGGA GCAGGCCAAG 
CTGCGACTGG AGATGGAGAT GGAACGTTTG CGTCAGACAC ATTTTAAGGA GATCGAAAGC 
AAGGATGATG AGGTGGAGGA GATCAGGCAG TTATGCAGTA AGAAGTTGAA GCAAATAGAG 
GTCCAGCTTG AAGAAGAGTA TGATGAAAAG CAGAAGGTGC TGAAGGAGAA GAGAGAGCTG 
GAGGCAAAGC TTCTGTCAGC ACAGAGCAAG GTGACAAACT GTGACATAGA CACTGAGAAA 
CGCCTGAAGA AGGACCTGAA GAGGACCAAG GCCCTGCTGG CTGATGCCCA GATCATGTTG 
GACCACATAA AGAACAACGC ACCCAGCAAG AGAGAGATCG CTCAGCTGAA AAACCAGCTG 
GAGGAGTCTG AGTTCACCTG TGCAGCTGCA GTCAAAGCTC GGAAATGCAT GGAGGTGGAG 
ATAGAAGACC TGCATGTTCA GATGGAGGAG ATCTCTAAAG CCAAGCAGAT GCTGGAAGAA 
CAGCAGAGCC GTCTTCAGAG AGAGAAGAAC GACCTGCAGT CCAGGACAGA GGAGGACCAG 
GAGGACCTCA ACGAGCTGAT GAAGAAACAC AAGGCTGCTG TGTCCCAGTT GGCTCAGAAC 
TTGGCTCAAA TGAACGACTT GCAGTCTCAG CTGGAGGAGG CCCTCAAGGA GAAACAGGAG 
GTTCAGGAAA AGATGCAGGT CCTCCAGAGC CAGCTGGAGT TCCAGGAGCA ATCCATGGTT 
GAGAAATCTC TAGCCAATCG TCAGGAGGCC AAGATCCGAG AACTGGAGAC CAAGATGGAG 
TTTGAGAAGT CGCAGGTCAA ACGACTGGAG AATCTTGTAG CTCGTCTGAA GGAGAACGTG 
GAAAAGCTGA CAGAAGAGCG AGACCAGCGC ATCAGCAGCG AGAATCGTGA GAAGGAGCAG 
AATAAGCGAC TGCAGAGACA GATCCGAGAC ATTAAAGAGG AAATGGGGGA ACTGGCCAAA 
AAAGAAGCAG AGGCAAGCCA CAAGAAACAC GAGCTGGAGA TGGACATTGA GAGCCTAGAG 
GCTTCAAACC AGAGCCTGCA GGCGGACCTT AAGCTGGCCT TCAAAAGGAT AGGTGACCTG 
CAGGCGGCCA TCGAGGACGA GATGGAGAGT GATGACAACG AAGACCTTAT AAACAGTCAG 
GGAAACTCAG ACACAGACTC TGAAGTGGAG GACCGTGTGG ATGGAGTGAA GTCCTGGTTG 
TCCAAAAATG ATGGCACTGT CAAGAACCTG TCAGATGAGG ACAGATGTGC TGTAAAAACC 
GACTTAAAGG AAGGAAAAGA GTATAAAGAG GCTAAGGAGA AACATAAAGA GCAGAGAGAG 
CGACAGACAA TCAGGCCGGT GCCTGTTGTG GCCTCTCTTA GTTATAGAAA AAAACCTGAC 
CTGGATTTCA TCATAGACAA AAGTGACTCC CTCCTCCGGT CTACCAAGAA AGCAGAGTCA 
GGCAACGTCC CAAGCAGATG CAAAACTGAG CTGCTAAACC AGAAATGTCA TGTTGACCCT 
TATCTAGCCT CTTCTAGGAG ACTTCCTCTT TCACTGGATG ACGTGAGTGA CAAACAGTCA 
GTCATCTCGC AGGCTGGTTC AGAGGCTAAT GGTAGCATCA GGAAAGGTAC GGACAGCCGT 
TGGGGTCAGT TTAATCTTTC ATTCACTAAG CCAGGATCAT CCTCCACCCT TCCTGAGATA 
AACCTGAGGA ATAAGGTACG TACTGGCAGC AGCTTGGGGC TATCAGGCCT ATACACACAC 
CATCAAAGCA TCTCTGGTAC AGACGGGGCC AGCAGCAGCC AGTGCCCTCC CAGTAGGAGA 
TCAAGAAGTC AAAGTCCTGG AAGTGTTGGC CAACTTGATT CCCAGGTATT TCTAGTTGGT 
AGCAGTTACC TCGGTGACGA TGGCATTGAC CTAGATGACA GTAGAAGTGT GGCCTTAACT 
GAGAGGTCCA TGTTCAGTCC TCTGTCTTCC ATTGGTCGCA GTTTATCAAT GCCTCCACCC 
AGAGACAGGC CCACTTCTGC TGATCTGCCA ATTGATACCG TGGACATTCG GCCGGTCAGC 
CATCAGAACT ACCTTGACCC TGACTTGGAG AAAGCCGTCA ATGAAGTGCT GAATTATAAA 
CCAATAAAAT TCAAGCGCAG GAGCTTGGAA GACTCTGAAG ATGATGAAAA ATCCAGGAGT 
AATGAAAATG AGAGCCGAAA GATGGAGAAT GGAGAGAGGA TGTATCCCAC CAGAGGTGCT 
AGATGTTCTG AATCTGCTGT GGATTGCAGG AGATCGTCCT GCAGTACCAG CAGCCATCAC 
CATGGCAGCA AGAGCAAAAG AAAGAGCAAG AAAAAGAGAA GTCACAGCTC TGAGTCCGAC 
AGTTCTGGAG ACAATCTTGT TGAAAGGACC AACTCTAAAA GGTGGCTCAA AAAGTCCCAA 
AAAATGTCCA AGAAAAAAGA GAAGCGTCCA TCATCGTCAG TATCTTCTGA ATCAGATTCT 
GAGTCAAACT TTACATCAAC TTCTGATGCA TCTGCCATTT CCTACCGGAG TTGCAGTAGT 
GTGAAAACAG CCGTGGGTAG ATCGGTTCCC AGTCCAGATG AGGAGGAGAC TCTGCCACCC 
AACAAGAAGC AGCCGATCAG TAAGAAGAAT GAGAAACAAA GGAAGAAGAA GGTGAACCAC 
CTGATGATGA AGTACTTGTA CCGGACAGAG AGTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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myo18a cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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