cep350 cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cep350
Entrez Gene ID	102228357
Full Name	centrosomal protein 350
Gene Type	protein-coding
Organism	Xiphophorus maculatus(southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023339105.1	XP_023194873.1	centrosome-associated protein 350 isoform X2
XM_014469315.1	XP_014324801.1	centrosome-associated protein 350
XM_023339104.1	XP_023194872.1	centrosome-associated protein 350 isoform X1

Pathways from BioSystems
Gene Ontology

The following cep350 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cep350 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXi05940	XM_014469315.1 Latest version!	Xiphophorus maculatus centrosomal protein 350kDa (cep350), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXi42336	XM_023339104.1 Latest version!	Xiphophorus maculatus centrosomal protein 350 (cep350), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXi42337	XM_023339105.1 Latest version!	Xiphophorus maculatus centrosomal protein 350 (cep350), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cep350 ( XM_014469315.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_014324801.1 Xiphophorus maculatus centrosomal protein 350kDa (cep350), mRNA.

Price & Availability↑

CloneID	OXi05940
Clone ID Related Accession (Same CDS sequence)	XM_014469315.1
Accession Version	XM_014469315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1445702400000
Organism	Xiphophorus maculatus(Southern platyfish)
Product	centrosome-associated protein 350
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005372228.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xiphophorus maculatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 48% of CDS bases ##RefSeq-Attributes-END##

ATGAGGAGCA GCAGGAGGAC TGAAGTACCC CATACAAGAT CTGCTCAACT GCTTACTGAT 
CACAATATTG GCACAGAGCT GACAACTGCA TGGAAAACTT TGGCCCAATC CAAAGCTGCT 
CTGCGGCATA TAGAAAATCG CTTGGAGGCC ACTCCAGGAA CTGCTGCTCT TCTGGACTCC 
ATCCCCATCT CCCCCAAGAA GAAAACATCA AGTTCATCTC GCAGCAGGGA TGGGCAACAA 
GGTGATGAAT CTAGAGGGAC TTCAAAACCC AGAGGTGGAA ATCAGCAGAG TCCAGCGAAG 
AGCAGCTCAC GCAGCCCTTT GAGAAACGCC ACTCAGGACA GCAATGTACG AAAGAACAGC 
AATGTGGAGT TCAGAGAACC TCTGGCCTCA TACAGAGAGG CCACCCCGCC TTCACATCCC 
TTTTCTCAGC TGGAGGCCTA CAATCTGCAG TCCGTGCCAG GGTCTTCATA CACATCCTCT 
CTGCCTAGCT CAGATCCTCT GCCCAGCCAG CTGATCTACC AGAGAGACAC CAGGGACAAG 
CAGACTGACG GGGACTTGGA CAGCATACGA TCCTCTGCAC TGGAGAGTGC TGAGGTGCGC 
TACCTCAATG ACCAGCCGGT CTTGGACAGC CTGAAGACAG AGCACAAAGC ACAACTTGTC 
ATGGATAGTC GTGAATCAAA GAGTTTTGCT CGGACGCCCA GCTCTTCACG CCAAGGGGAG 
AGCACTCCTT CCTCAAGTCC GGGCTCAGTT ACCCAACGGT TGGAAAACCT GAGACGACAT 
CAGCCAGATG ATAAATTAGA AAAACTAAAG GAGCGCATTC GACGACAGAG GCAACATTTA 
GAGGAGACAG CAGACAAGGA AAAGTCTAAG AGTTGTCTTG AAAAGCCCAT TGTTGCGTTT 
GTTGAGGGTG ACAATGTTGG TTCAAGCAGC ATGCCAGCAG CAAAGATACG GAAAGTCACA 
GCAGCACCGC CAGCGCCTAT ATACAAAGGT TTCAACAGTG CAGAGACAAA GGTCAAAACT 
CCTGATGGTA AAGTTTGGAA AGAGGAGGAA TTCCATAACG TCAACAGAAA AGTTTACAGA 
GACATATCAC ACCAATTTTC TGAGAGCAGC AAATTCAGGC ATCAGCATCA GAGAACAGAA 
CGGTCCAAGG AGAAGAGGCC ATCCAAACCA GTCAGGAAGG TCCAAAAAGT TGCCCTGGTA 
TCTGAGCAGA ATTTTAAACC AGTGATTAGT CCTGCATCAT GGCGTGAGGG CCAAAAACTG 
GTAAAGATGG TACTAGGTCC TGCTCCAAAG CTACCCAGTG AAGAGCACAG TTCTCATCCA 
GCTGGCAGAC AGACCCGAAC AGCTTCCCAT CACCACTCAG ACCCACATTC AGAACAGAGG 
AGACGCTCTC GGCCAAACAG CACAGACAGG TCTCGCAGTG CTTTTCAGGA GCAAATGGGC 
AGCCGCCCTA CAGCCTCATA CAGTCCCTCT TCTTCAGCTC AGAACAAGAT GCCACGGGGA 
GTGAAGACAG ATGTTTTGTC AGTAGACATC CAGGGGATTC TTGATGACCT TCAGCTGGAT 
TGCAAATCAG CTGAAATAGA AGAGCGTGCT CATAAAAGAT CCCGGGGTGT CAGTGGTAGT 
AGAAGAGGAA GAGGAGGGTC AGGTTCTCGG ACGAGGGCAC CAGTCTCTGC TTGGGGATCT 
ACAGGAGTGG CGAACACCAG GTCCAGGGTC TGCCGGAGTG CTAGCCCCAC CACATGCCAG 
CCTGACAATA CTGACAAAAC AAGCAAAAGT GTCAAGAAGC GGCACTATGA CGCAGATACA 
GTTCGCCAAT ACATTGCACA ACAACAAGAG GAGAGAAAGA GGCGACAGGC AGAAGAGAAG 
AGAGCAGAAA AAGAGGAGAC AGCAAAAAGA AGTCAGAGAT TGCAGGAGCT GTACAAGAAA 
CAAAAAGAAG TAGTTAGAAC TGCGACCCTT TCCTCTGAGG CAGTTGTGGG TCCTGTTCAA 
AAGCGACTGC AGGAGACCTA CACCAGACTG CTTCTGGAGG ATGCCCGATT CAGCGAGACA 
ACACCTGAGC AACATTCTAC ACCTTTCATT CAGATGAGAC CCATGTACCA GCCTTCAGGA 
GAGTCGGACA AAGAAAACAA AAGAATCGGG GTTTCACAAA GCCCCTCAAG CAGTGACAAG 
TCTTTAAATG AGCAGCCGCC ACTGCCATTA TTCAGGAATG ATCTTGGGTT TACCTCTGTG 
CTTCAGCCTG ATCATCTGAA TCCAGCTTTT CAACCCACCA GCAGTGTACA AGTAGCTTCT 
AATGAACATC TTCTCTCTCA GCTTTTAAGG CTGGAGGCAG CATTAGCCTC TAGTAACAGA 
AAGTACACTG AACAGCCGGC TGCAGCGCCT CACCTCCTGT CCCCGACCAG AATGTCTCGT 
ATTGAAGCCC TGAAGGCAAA GGCCACATCC CTCTCAAACC GTATCGAAAC GGAGGCCCGA 
AGGTTTGCAG GACAAGGGAT CAACTATGGT GCAGCAACTT CCATGGATGT TGATACCGTT 
TTGCAACCCA GAACCTCTAG CCTTGATGTT GATCACTGGA CAGGAACCGT TGCTCATGCT 
ACAGAAAATA GCGACTTCTC CTTTAGGGCA CAGAAAGCGT TCAAAAATGC AGCTAATACT 
TCATATACTG GCATAGTTCT TCCAGGAGCA GGGAATCCGC ACGACTTAAA TGAAAAGACA 
CACACCAGTG TGACAAATCC ACATACTGGT TCTCCTGGTG TAAATGGGTT AACTTTAAAC 
ACTTACACCC AAGAAGATAG GAGGATTTTT GGTGACTTGG ACATGGAAGA GAGAACAGAA 
AAACTAGTAC AAAGAATAAA AACAAAAAAA CCGGAGAATC TGGTGTATCC GTATCAGACT 
GATCTCCACG ATTCAAGTGC TGGTTCCATT AGTGAGGGTC CTCTTCTTAG TGAAGGAAGT 
TTTTCTGAGG AAGATGGAAG TCCTCCTCAC TACACCAGTA ACCATGGGTG TAATTTAGAT 
TACTTGGAGG CTGAGGACTA CAGCGCAGGT AAAAGAAGTC ATAACAAGCG TCTGTTTGAG 
TTCCAGAGGG AGGCAGCACA GTATACAGCC CTCAGCCCGC CCTTTACACA ACTTAATGGC 
AGCAAAGCAC CATGGGAAGA GCTGAACAAA GGAAGTCCTC TGAGCGTAAT CAATATTTTC 
ACAAAAAATG TGCAAGGCCA TGTTAAAGTG AGTGAGAGAG CTTCAGGGAA TAATTCTCCA 
CTTCCTCATT CTTCACACTC CGAAAACAGC CAAGATGAAG CAGCAGTCTA TGAGGATGAC 
TTTGTTTCAT CACATAGCAG CAAAACCAGC AGCCATCTAA AGAGAGGCCC CAAGTCGCCC 
AGACCCAAAC TGGTTTCCTC GTCCTGTTCT CCAGACCTCA GTTCTTCTCT AACGGCAGAC 
TCTCTCAGTG AGCATGTGAG AAGAAGCTCT CATAATCCAA AAACATCCAG CAGAGCAGAG 
TCATGGTCCA CAGGTGAGCT ACAATATTCC CCTGCTGTCT TGCAGCAGCG CATGGCAGCA 
GAACTGCAGT ACTTAGAGTC CATTGAAGAG TCAATCAAGC AGCTGGGAGA CGTGGAGAAG 
CTGATGGGTG TGTCCATGGC ACAACAGGAG AGTGCGTCAT TGACCCAAAT GCTCAAGGCC 
AAGCAGCACC GCCATGAGCA GGAGCTCTAT GAGCTGAAGA TCAAGACTGA AAGAGAAGCT 
CTCGAGACAA AACTCCAACT AGAAGAAAAC AGACAGAGGG TTGCCAGAGC TCATTTAGAG 
CTGCAGGAAA ACTTGGCAAC ATCACAAAAG CAAACTTTGG AAGGTCTCCA AGAGTCAACT 
TCTAAGATGA TGAGCCAACA GACTGAAGCA GCACGATACA CAGCAGATGC TGCCCGACAC 
ATTAAGGAGA TGACAGACCT GGCACGATCC CAGATTGCGG GGCATTTGAT AATCCCACCT 
GTTGCCACTG ATTCTGCGAT GTTGGTCCAG CAGGAAGAAC TAAGTAGTTC CTACACAAAG 
CAGCAAGACA AGGCAGACTC TAGCAGATTT AAAAGTGAGG TTTCAGACCG CAGGACCAGG 
TCTGGAGAGC CCTCAGCTTC ACAAAACGGC CTCAGTCAGT CTGACTCTCC ATCTTTCAGA 
AGACCAAACA TCAGTGGATC TCCATATGGA AGCATCCAAA CCAGTCCATC ACATGCTCCA 
TCTGATCACA CAAAAAGGCT GAACACACAG TCTGGTGAGA GAAAACAGAA GAATGAGAGA 
GTTGAGGAGA GGATGAAGGC GGAAGCAGCC AGCTGCTCTA TTGAGGAGGA TGTTGTAACA 
GCAGCAAATG ACTCACTCTG CAGCGAAAGT ATTGCCTCTG CAGGGGACGA GAAAGACACT 
ACATCTGTGG CCACTGAGTA CTCTCTAAAG TTCGATGAGT CCATGACGGA GGACGAGATA 
GAGGAGCGCT CTTTCCGTTC TCTGCTGCCG TCCGAAGCTC ATCGTCGGGG AACTATGGAG 
AAAAAGTCGC ACCATCAGAA GGAGGGGGAC GCAGTCTGCC TCAATGAAAT GTTGGTCCCA 
GAGACGGGGT CATGCAGTTC CTTCAAGTCT CATGATGCAC ACATAGCCTT TTCTGGTGGG 
CAGGACAGCT TTTCTCAGTT CACAATGGAA ATGGTGCGTC AGTACATGAG GGATGAAGAG 
GTAAGATTGC AGCACCAGAG CTCTCTGCTC CATCTACGGC AGAAAGCGCT TAAGGAAAAG 
ACGAGGACAG AAGTAGCCTG GCTTGAGCAC CAAAAGAAAA AGCTGAGAGA CAAAGGAGAG 
GATGACAAAA TGCCACCAAT CAGGAAGAAG CAGAGGGGAC TTCTTCTGAA ACTCCAACAG 
GAGCAAGCTG AGATCAGGAG ACTCCAGGAA GCCAACAGAG CAGCTAGAAA AGAAAGGCAG 
TTGTTGCTGA AACAGCAGGA AGAGATTGAA CGGATGAGAA ACTCAACACT TAGACTGAAG 
GAGCGTCTCA AGTGTGCAGA GGGAGACATT CCCCCTACGC CAGTGTCAGA GCCTTCAGGG 
TCTGAGCTCT CACAGAGACT TCCTGGTGAA GTCCGCACCT CCTCTCCTTC ACTCTCCATT 
TCTGGAAGTG AGACCAGCAG CATCATGCAG AAGCTTAAGA AAATGCGATC TCACATGGAT 
GAAAAGTTTC TGACCAAGCG CGAGCAGCAG CTGATGCAAA GGCGACACCA TGCAGAGGAG 
CTGCTCCAGT GGAAACAGCA ACTGGACAAG GAGGAGGCGG AGGTACGAAG GATGGAGAAA 
GAGGCCGTGG CCGCCTGGGA CCAGAGAAGA CCTCGGGACA AGGACCATAA AACACCTGAG 
AGCATTGGAA AAGAGATCAG TGAAAATGAA CTGCAGGCCT CTAACCACCA AAGCTTAGAG 
GCCAGATCTG ACAGTGAGAA GGAGCTTGTA AGTGAGGACA ATTGCTCCTC AATTACAACC 
GAGTCCAGTA TACACACAGA GGCTCCGGCT TCTCAGCACC CACTCAGTGC GTCCTCAGCA 
CAGCCAGCTT CAGTCGCTGA AACACCTCTG GCCTCAGTTC AGAACAGCCA TGCTACCTAC 
ACACAGGACT TTTCCACTGA GTCACAGTTA CCTGCTGTAA AGTCCTCTTC AGTCGAAGGC 
AGCCGCAGCC CTGCTGCTTC TCCATTAGAC AGCAGCAGTA AGACCAAGAT GCAACCCCGC 
TCCACCTCCC GGACACACAG TGAGGTTTTC CCTGAGGTCC TTGGAGCCGC CACAGCTATG 
CCGTCTGACC AGAGTGACAT TGAGAGTCGT ATCAAGGCAC TGAGGGAAGA ACTGAAGAAA 
CGTAAGATCA TGGCCCACCA GCTGAAAAAA GAGCAGAAGA AGCGGCACAA GGAGCGCCTG 
AAGGCACAGG AGGCAACTCT GTTGAAGAAA CTGGAGAACT ACAACAATTT TATTGAGAAA 
ACGAAGGCGG AACTGAACAA AGAGCCAGAC TCAACTCCTG AAACGACGTC ACGCATAAAT 
GATTCCAACT ACATTTTGGA GCAGTCATCT GTTAAACCCT CTTCACACAG GTCTGACGCG 
ATCAAAAGCT CCGCCTCTGA CCGGACGCTT ATTGATGCAT CATTAGATCA CAATGGCATT 
CCTCAGCCGA ACCATAGCAG AACCGCATCC AGAGATTTAC CCGAAGATTC TCCCAGTGTC 
GTCGTGTACA GCAAACCAGA GAGCGATCAT CTATCAGAGC CTCTCTCAAG ATCTTTTTTC 
AAGCCAGAGT CTGAGGAGGA GAAGGCTTTG TCTGATCACA GATCTGACAT TGAGGAAGAG 
TTGGATGTGG AAGTGAACTC CAGGTCTGAG GATGAACAGT CTCACCACCT TCTCAGACTC 
GACTCTCAGG AGAACTTAAC TGCATCTGAA CGTGTTCCGC CTTTTGCTAA GGAGGAACAT 
GAGCAGTCTC TGTCTGGCAG TTTAGATCAG GAAGAGCTGA AGAAACAAAA CAAAATACCC 
GAGGCGGAGG AAGCTGTGAC GTTAAATAAT ATTACACCCG GAACTCCTTT TTCAGCTGTT 
AACCTGGAAG CTTCACCACA TTTAAGCCGC AGCCCTTCAC CCACTGAGCA CCCTCTCTCC 
CCACACTCAG TGGGTTTCTC ACTCAATAAA GATCCACTCA TAAAGGAAGT TGAGGCTTCT 
GTTGGAACAG ACGGTTACCA GGAGGACTTT GAATCAGCTA ATTCTTCCCC TAGGGACGAC 
CATATTTCTA AGCATCAGTC TCAAACCTCC ATTCCCCACA CAGAAACTAA GCATTCAACT 
AAGGAATCAT CCAGGGCAAC CCCTCCATTT GACCATCAAG ATGAAGAAGA AGAAATACCT 
GAGGAGATGA GTCAGCATTC TGGCATCAGT GAAAAAAGCC ATCATTCAGA AAAGTTACTG 
GAACTCCAGA AGAAGGAAGA AGATATAAAA TTAGACAATA AGACAGAGTT TCCCAGCCAT 
GCACCTGCTT CTGCTCCTCC CATCGAAGAT GAAATGCTAG GTTTCCACCT TGGAGACCGG 
GTTCTTGTTG GAGGTGTTCA GCCGGGCACA CTGAGATTTA AAGGTCCGAC CAGCTTTGCC 
AATGGCTTCT GGGCTGGCGT GGAGTTGGAC AAATCTGAAG GGAGCAACAA TGGCACTTAT 
GATGGAGTTG TTTACTTTGA GTGTGAGGAA TCGCACGGCA TCTTTGCTCC TCCAGACAAA 
GTCTCCCATC TCCCAGACAA GTTTGAGCTC TATGCAGATA CCACAGAAGA TGAGGACTCT 
TTCTTCGACG ACTTATCTGA TAAAGGCGGG AAAAAACACA AAAAGATTAA AGACGATTCT 
CAAAAACGGG AAGATCCAGA AATGCGAAAT GAACAAACAT CTGACAAGGA AACTAAATCT 
AAAGATGAAA TGGTTTTGGA TGAGAATGAT CAGATTCCAC TCAAGACCAA AGCCCACCTC 
AATTCACAGC ACCAGAAAGA ACCTGAGCAT CAGATTTGTA ACGGCAACAT GAAGGACATC 
ATTTTGGACT TTACTGATGA ATCCCGCACA TTCCTCATCC CGGACAAAGA TAGAATGGAG 
GAGAAGAATG AGAAGACGAC CGCCATTATT GAGACAAACG ATGTTGACAT CGAGAAGGAC 
TTGATTCCTA CGGATGAAAT CAAGACTGAT AAGAGAGAAC AGAAGAACAG GGATCTGGTG 
GAGACATTTG CAGATGAGCT CATCAACAAC TTTATCAAAG ATTCCTTTAA GCAATGTGCT 
GAAATAAAGA GGGCTAAAGA AGGAAAGATC AGAAATGCCA ACCAGTTAAA TGGATGTTCG 
TTTGTTGAAA ATAACGACAA GAAATGGGTC TCTTCAGTGG AGCAAAAAGA CAACCTGCCC 
TTCTTTCTGC CGGCTGAGAA GGAGGAGCTG TCTTCTCCTG AACTGTGCAA CAGATCAGAG 
AGTCCAGTGT TTGGCCCCAG TGGTCAAGAG AAGCTTGCTA AGCGTCTGGC TGAGCTGGAG 
CTGAGTCGTG AGCTGCTGGA GGATCTCGGT GATGATCAGG ACTGGTTCGA TGAGGACTTT 
GGGCTCAGCT CACGGAGACA GCAGCAGAGA CTCAAACAGA AAGAAGAGCT GGTACCCCTC 
CCCTTACCTC CCAAACTACC TGAGCAGCCC GCCATGGTTG TGCCCCACTC GGCCACAGAG 
GTGGAGAAAA TGGTCCATTC TGCCACACAG GAGATCTGGG AGACCTTTCA TCTGGGAAGG 
GAGGGGGCTC TTATCCTTGA ACATCTTCCA AAGCCGACGC CTTCCCTGGA GTATTTAGGT 
AAAGAGTCCA GCAGCCAGGA CCAGGAAGCC CTGTCCATCA GCAGCTACAA AAAGGCTGTG 
TATGACCTGA CATGGGAGAT ACTGAAGGAG ATTTTTGCTG AAGACCCAAA TACTGATCAG 
CCTCAGTGGG TCAAACCACG CCAGGTCAAG TCCTCCTCCA TTCACAGAGT CAAGACGTCT 
GGAGACATCA GCAAAATTCA GGAATTTGTC ACAGAAGAAG TGTTGAAACT GTATGGTCTG 
ACACAAGATG AGAATCAAAA GACCGACTGG CAGAAAATGC TCAAATTTGG CAGGAAGAAG 
CGGGACAGAG TCGATCACAT ACTGGTCCAG GAACTCCACG AGGAAGAGGC CCAGTGGGTT 
AACTACGACG AGGACGAACT GTTTGTCAAG ATTCAGCTGG CAGACAGCAT CTTTGAGGCT 
TTGCTGAAGG ACACTGTAAA CATTTTCACA CTAATTTCTG ACAAGAGAGC CAAAAGAGGA 
GCTCTCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014324801.1
CDS	1..8772
Translation	MRSSRRTEVPHTRSAQLLTDHNIGTELTTAWKTLAQSKAALRHIENRLEATPGTAALLDSIPISPKKKTSSSSRSRDGQQGDESRGTSKPRGGNQQSPAKSSSRSPLRNATQDSNVRKNSNVEFREPLASYREATPPSHPFSQLEAYNLQSVPGSSYTSSLPSSDPLPSQLIYQRDTRDKQTDGDLDSIRSSALESAEVRYLNDQPVLDSLKTEHKAQLVMDSRESKSFARTPSSSRQGESTPSSSPGSVTQRLENLRRHQPDDKLEKLKERIRRQRQHLEETADKEKSKSCLEKPIVAFVEGDNVGSSSMPAAKIRKVTAAPPAPIYKGFNSAETKVKTPDGKVWKEEEFHNVNRKVYRDISHQFSESSKFRHQHQRTERSKEKRPSKPVRKVQKVALVSEQNFKPVISPASWREGQKLVKMVLGPAPKLPSEEHSSHPAGRQTRTASHHHSDPHSEQRRRSRPNSTDRSRSAFQEQMGSRPTASYSPSSSAQNKMPRGVKTDVLSVDIQGILDDLQLDCKSAEIEERAHKRSRGVSGSRRGRGGSGSRTRAPVSAWGSTGVANTRSRVCRSASPTTCQPDNTDKTSKSVKKRHYDADTVRQYIAQQQEERKRRQAEEKRAEKEETAKRSQRLQELYKKQKEVVRTATLSSEAVVGPVQKRLQETYTRLLLEDARFSETTPEQHSTPFIQMRPMYQPSGESDKENKRIGVSQSPSSSDKSLNEQPPLPLFRNDLGFTSVLQPDHLNPAFQPTSSVQVASNEHLLSQLLRLEAALASSNRKYTEQPAAAPHLLSPTRMSRIEALKAKATSLSNRIETEARRFAGQGINYGAATSMDVDTVLQPRTSSLDVDHWTGTVAHATENSDFSFRAQKAFKNAANTSYTGIVLPGAGNPHDLNEKTHTSVTNPHTGSPGVNGLTLNTYTQEDRRIFGDLDMEERTEKLVQRIKTKKPENLVYPYQTDLHDSSAGSISEGPLLSEGSFSEEDGSPPHYTSNHGCNLDYLEAEDYSAGKRSHNKRLFEFQREAAQYTALSPPFTQLNGSKAPWEELNKGSPLSVINIFTKNVQGHVKVSERASGNNSPLPHSSHSENSQDEAAVYEDDFVSSHSSKTSSHLKRGPKSPRPKLVSSSCSPDLSSSLTADSLSEHVRRSSHNPKTSSRAESWSTGELQYSPAVLQQRMAAELQYLESIEESIKQLGDVEKLMGVSMAQQESASLTQMLKAKQHRHEQELYELKIKTEREALETKLQLEENRQRVARAHLELQENLATSQKQTLEGLQESTSKMMSQQTEAARYTADAARHIKEMTDLARSQIAGHLIIPPVATDSAMLVQQEELSSSYTKQQDKADSSRFKSEVSDRRTRSGEPSASQNGLSQSDSPSFRRPNISGSPYGSIQTSPSHAPSDHTKRLNTQSGERKQKNERVEERMKAEAASCSIEEDVVTAANDSLCSESIASAGDEKDTTSVATEYSLKFDESMTEDEIEERSFRSLLPSEAHRRGTMEKKSHHQKEGDAVCLNEMLVPETGSCSSFKSHDAHIAFSGGQDSFSQFTMEMVRQYMRDEEVRLQHQSSLLHLRQKALKEKTRTEVAWLEHQKKKLRDKGEDDKMPPIRKKQRGLLLKLQQEQAEIRRLQEANRAARKERQLLLKQQEEIERMRNSTLRLKERLKCAEGDIPPTPVSEPSGSELSQRLPGEVRTSSPSLSISGSETSSIMQKLKKMRSHMDEKFLTKREQQLMQRRHHAEELLQWKQQLDKEEAEVRRMEKEAVAAWDQRRPRDKDHKTPESIGKEISENELQASNHQSLEARSDSEKELVSEDNCSSITTESSIHTEAPASQHPLSASSAQPASVAETPLASVQNSHATYTQDFSTESQLPAVKSSSVEGSRSPAASPLDSSSKTKMQPRSTSRTHSEVFPEVLGAATAMPSDQSDIESRIKALREELKKRKIMAHQLKKEQKKRHKERLKAQEATLLKKLENYNNFIEKTKAELNKEPDSTPETTSRINDSNYILEQSSVKPSSHRSDAIKSSASDRTLIDASLDHNGIPQPNHSRTASRDLPEDSPSVVVYSKPESDHLSEPLSRSFFKPESEEEKALSDHRSDIEEELDVEVNSRSEDEQSHHLLRLDSQENLTASERVPPFAKEEHEQSLSGSLDQEELKKQNKIPEAEEAVTLNNITPGTPFSAVNLEASPHLSRSPSPTEHPLSPHSVGFSLNKDPLIKEVEASVGTDGYQEDFESANSSPRDDHISKHQSQTSIPHTETKHSTKESSRATPPFDHQDEEEEIPEEMSQHSGISEKSHHSEKLLELQKKEEDIKLDNKTEFPSHAPASAPPIEDEMLGFHLGDRVLVGGVQPGTLRFKGPTSFANGFWAGVELDKSEGSNNGTYDGVVYFECEESHGIFAPPDKVSHLPDKFELYADTTEDEDSFFDDLSDKGGKKHKKIKDDSQKREDPEMRNEQTSDKETKSKDEMVLDENDQIPLKTKAHLNSQHQKEPEHQICNGNMKDIILDFTDESRTFLIPDKDRMEEKNEKTTAIIETNDVDIEKDLIPTDEIKTDKREQKNRDLVETFADELINNFIKDSFKQCAEIKRAKEGKIRNANQLNGCSFVENNDKKWVSSVEQKDNLPFFLPAEKEELSSPELCNRSESPVFGPSGQEKLAKRLAELELSRELLEDLGDDQDWFDEDFGLSSRRQQQRLKQKEELVPLPLPPKLPEQPAMVVPHSATEVEKMVHSATQEIWETFHLGREGALILEHLPKPTPSLEYLGKESSSQDQEALSISSYKKAVYDLTWEILKEIFAEDPNTDQPQWVKPRQVKSSSIHRVKTSGDISKIQEFVTEEVLKLYGLTQDENQKTDWQKMLKFGRKKRDRVDHILVQELHEEEAQWVNYDEDELFVKIQLADSIFEALLKDTVNIFTLISDKRAKRGALS

Target ORF information:

RefSeq Version	XM_014469315.1
Organism	Xiphophorus maculatus(Southern platyfish)
Definition	Xiphophorus maculatus centrosomal protein 350kDa (cep350), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014469315.1

ORF Insert Sequence:

ATGAGGAGCA GCAGGAGGAC TGAAGTACCC CATACAAGAT CTGCTCAACT GCTTACTGAT 
CACAATATTG GCACAGAGCT GACAACTGCA TGGAAAACTT TGGCCCAATC CAAAGCTGCT 
CTGCGGCATA TAGAAAATCG CTTGGAGGCC ACTCCAGGAA CTGCTGCTCT TCTGGACTCC 
ATCCCCATCT CCCCCAAGAA GAAAACATCA AGTTCATCTC GCAGCAGGGA TGGGCAACAA 
GGTGATGAAT CTAGAGGGAC TTCAAAACCC AGAGGTGGAA ATCAGCAGAG TCCAGCGAAG 
AGCAGCTCAC GCAGCCCTTT GAGAAACGCC ACTCAGGACA GCAATGTACG AAAGAACAGC 
AATGTGGAGT TCAGAGAACC TCTGGCCTCA TACAGAGAGG CCACCCCGCC TTCACATCCC 
TTTTCTCAGC TGGAGGCCTA CAATCTGCAG TCCGTGCCAG GGTCTTCATA CACATCCTCT 
CTGCCTAGCT CAGATCCTCT GCCCAGCCAG CTGATCTACC AGAGAGACAC CAGGGACAAG 
CAGACTGACG GGGACTTGGA CAGCATACGA TCCTCTGCAC TGGAGAGTGC TGAGGTGCGC 
TACCTCAATG ACCAGCCGGT CTTGGACAGC CTGAAGACAG AGCACAAAGC ACAACTTGTC 
ATGGATAGTC GTGAATCAAA GAGTTTTGCT CGGACGCCCA GCTCTTCACG CCAAGGGGAG 
AGCACTCCTT CCTCAAGTCC GGGCTCAGTT ACCCAACGGT TGGAAAACCT GAGACGACAT 
CAGCCAGATG ATAAATTAGA AAAACTAAAG GAGCGCATTC GACGACAGAG GCAACATTTA 
GAGGAGACAG CAGACAAGGA AAAGTCTAAG AGTTGTCTTG AAAAGCCCAT TGTTGCGTTT 
GTTGAGGGTG ACAATGTTGG TTCAAGCAGC ATGCCAGCAG CAAAGATACG GAAAGTCACA 
GCAGCACCGC CAGCGCCTAT ATACAAAGGT TTCAACAGTG CAGAGACAAA GGTCAAAACT 
CCTGATGGTA AAGTTTGGAA AGAGGAGGAA TTCCATAACG TCAACAGAAA AGTTTACAGA 
GACATATCAC ACCAATTTTC TGAGAGCAGC AAATTCAGGC ATCAGCATCA GAGAACAGAA 
CGGTCCAAGG AGAAGAGGCC ATCCAAACCA GTCAGGAAGG TCCAAAAAGT TGCCCTGGTA 
TCTGAGCAGA ATTTTAAACC AGTGATTAGT CCTGCATCAT GGCGTGAGGG CCAAAAACTG 
GTAAAGATGG TACTAGGTCC TGCTCCAAAG CTACCCAGTG AAGAGCACAG TTCTCATCCA 
GCTGGCAGAC AGACCCGAAC AGCTTCCCAT CACCACTCAG ACCCACATTC AGAACAGAGG 
AGACGCTCTC GGCCAAACAG CACAGACAGG TCTCGCAGTG CTTTTCAGGA GCAAATGGGC 
AGCCGCCCTA CAGCCTCATA CAGTCCCTCT TCTTCAGCTC AGAACAAGAT GCCACGGGGA 
GTGAAGACAG ATGTTTTGTC AGTAGACATC CAGGGGATTC TTGATGACCT TCAGCTGGAT 
TGCAAATCAG CTGAAATAGA AGAGCGTGCT CATAAAAGAT CCCGGGGTGT CAGTGGTAGT 
AGAAGAGGAA GAGGAGGGTC AGGTTCTCGG ACGAGGGCAC CAGTCTCTGC TTGGGGATCT 
ACAGGAGTGG CGAACACCAG GTCCAGGGTC TGCCGGAGTG CTAGCCCCAC CACATGCCAG 
CCTGACAATA CTGACAAAAC AAGCAAAAGT GTCAAGAAGC GGCACTATGA CGCAGATACA 
GTTCGCCAAT ACATTGCACA ACAACAAGAG GAGAGAAAGA GGCGACAGGC AGAAGAGAAG 
AGAGCAGAAA AAGAGGAGAC AGCAAAAAGA AGTCAGAGAT TGCAGGAGCT GTACAAGAAA 
CAAAAAGAAG TAGTTAGAAC TGCGACCCTT TCCTCTGAGG CAGTTGTGGG TCCTGTTCAA 
AAGCGACTGC AGGAGACCTA CACCAGACTG CTTCTGGAGG ATGCCCGATT CAGCGAGACA 
ACACCTGAGC AACATTCTAC ACCTTTCATT CAGATGAGAC CCATGTACCA GCCTTCAGGA 
GAGTCGGACA AAGAAAACAA AAGAATCGGG GTTTCACAAA GCCCCTCAAG CAGTGACAAG 
TCTTTAAATG AGCAGCCGCC ACTGCCATTA TTCAGGAATG ATCTTGGGTT TACCTCTGTG 
CTTCAGCCTG ATCATCTGAA TCCAGCTTTT CAACCCACCA GCAGTGTACA AGTAGCTTCT 
AATGAACATC TTCTCTCTCA GCTTTTAAGG CTGGAGGCAG CATTAGCCTC TAGTAACAGA 
AAGTACACTG AACAGCCGGC TGCAGCGCCT CACCTCCTGT CCCCGACCAG AATGTCTCGT 
ATTGAAGCCC TGAAGGCAAA GGCCACATCC CTCTCAAACC GTATCGAAAC GGAGGCCCGA 
AGGTTTGCAG GACAAGGGAT CAACTATGGT GCAGCAACTT CCATGGATGT TGATACCGTT 
TTGCAACCCA GAACCTCTAG CCTTGATGTT GATCACTGGA CAGGAACCGT TGCTCATGCT 
ACAGAAAATA GCGACTTCTC CTTTAGGGCA CAGAAAGCGT TCAAAAATGC AGCTAATACT 
TCATATACTG GCATAGTTCT TCCAGGAGCA GGGAATCCGC ACGACTTAAA TGAAAAGACA 
CACACCAGTG TGACAAATCC ACATACTGGT TCTCCTGGTG TAAATGGGTT AACTTTAAAC 
ACTTACACCC AAGAAGATAG GAGGATTTTT GGTGACTTGG ACATGGAAGA GAGAACAGAA 
AAACTAGTAC AAAGAATAAA AACAAAAAAA CCGGAGAATC TGGTGTATCC GTATCAGACT 
GATCTCCACG ATTCAAGTGC TGGTTCCATT AGTGAGGGTC CTCTTCTTAG TGAAGGAAGT 
TTTTCTGAGG AAGATGGAAG TCCTCCTCAC TACACCAGTA ACCATGGGTG TAATTTAGAT 
TACTTGGAGG CTGAGGACTA CAGCGCAGGT AAAAGAAGTC ATAACAAGCG TCTGTTTGAG 
TTCCAGAGGG AGGCAGCACA GTATACAGCC CTCAGCCCGC CCTTTACACA ACTTAATGGC 
AGCAAAGCAC CATGGGAAGA GCTGAACAAA GGAAGTCCTC TGAGCGTAAT CAATATTTTC 
ACAAAAAATG TGCAAGGCCA TGTTAAAGTG AGTGAGAGAG CTTCAGGGAA TAATTCTCCA 
CTTCCTCATT CTTCACACTC CGAAAACAGC CAAGATGAAG CAGCAGTCTA TGAGGATGAC 
TTTGTTTCAT CACATAGCAG CAAAACCAGC AGCCATCTAA AGAGAGGCCC CAAGTCGCCC 
AGACCCAAAC TGGTTTCCTC GTCCTGTTCT CCAGACCTCA GTTCTTCTCT AACGGCAGAC 
TCTCTCAGTG AGCATGTGAG AAGAAGCTCT CATAATCCAA AAACATCCAG CAGAGCAGAG 
TCATGGTCCA CAGGTGAGCT ACAATATTCC CCTGCTGTCT TGCAGCAGCG CATGGCAGCA 
GAACTGCAGT ACTTAGAGTC CATTGAAGAG TCAATCAAGC AGCTGGGAGA CGTGGAGAAG 
CTGATGGGTG TGTCCATGGC ACAACAGGAG AGTGCGTCAT TGACCCAAAT GCTCAAGGCC 
AAGCAGCACC GCCATGAGCA GGAGCTCTAT GAGCTGAAGA TCAAGACTGA AAGAGAAGCT 
CTCGAGACAA AACTCCAACT AGAAGAAAAC AGACAGAGGG TTGCCAGAGC TCATTTAGAG 
CTGCAGGAAA ACTTGGCAAC ATCACAAAAG CAAACTTTGG AAGGTCTCCA AGAGTCAACT 
TCTAAGATGA TGAGCCAACA GACTGAAGCA GCACGATACA CAGCAGATGC TGCCCGACAC 
ATTAAGGAGA TGACAGACCT GGCACGATCC CAGATTGCGG GGCATTTGAT AATCCCACCT 
GTTGCCACTG ATTCTGCGAT GTTGGTCCAG CAGGAAGAAC TAAGTAGTTC CTACACAAAG 
CAGCAAGACA AGGCAGACTC TAGCAGATTT AAAAGTGAGG TTTCAGACCG CAGGACCAGG 
TCTGGAGAGC CCTCAGCTTC ACAAAACGGC CTCAGTCAGT CTGACTCTCC ATCTTTCAGA 
AGACCAAACA TCAGTGGATC TCCATATGGA AGCATCCAAA CCAGTCCATC ACATGCTCCA 
TCTGATCACA CAAAAAGGCT GAACACACAG TCTGGTGAGA GAAAACAGAA GAATGAGAGA 
GTTGAGGAGA GGATGAAGGC GGAAGCAGCC AGCTGCTCTA TTGAGGAGGA TGTTGTAACA 
GCAGCAAATG ACTCACTCTG CAGCGAAAGT ATTGCCTCTG CAGGGGACGA GAAAGACACT 
ACATCTGTGG CCACTGAGTA CTCTCTAAAG TTCGATGAGT CCATGACGGA GGACGAGATA 
GAGGAGCGCT CTTTCCGTTC TCTGCTGCCG TCCGAAGCTC ATCGTCGGGG AACTATGGAG 
AAAAAGTCGC ACCATCAGAA GGAGGGGGAC GCAGTCTGCC TCAATGAAAT GTTGGTCCCA 
GAGACGGGGT CATGCAGTTC CTTCAAGTCT CATGATGCAC ACATAGCCTT TTCTGGTGGG 
CAGGACAGCT TTTCTCAGTT CACAATGGAA ATGGTGCGTC AGTACATGAG GGATGAAGAG 
GTAAGATTGC AGCACCAGAG CTCTCTGCTC CATCTACGGC AGAAAGCGCT TAAGGAAAAG 
ACGAGGACAG AAGTAGCCTG GCTTGAGCAC CAAAAGAAAA AGCTGAGAGA CAAAGGAGAG 
GATGACAAAA TGCCACCAAT CAGGAAGAAG CAGAGGGGAC TTCTTCTGAA ACTCCAACAG 
GAGCAAGCTG AGATCAGGAG ACTCCAGGAA GCCAACAGAG CAGCTAGAAA AGAAAGGCAG 
TTGTTGCTGA AACAGCAGGA AGAGATTGAA CGGATGAGAA ACTCAACACT TAGACTGAAG 
GAGCGTCTCA AGTGTGCAGA GGGAGACATT CCCCCTACGC CAGTGTCAGA GCCTTCAGGG 
TCTGAGCTCT CACAGAGACT TCCTGGTGAA GTCCGCACCT CCTCTCCTTC ACTCTCCATT 
TCTGGAAGTG AGACCAGCAG CATCATGCAG AAGCTTAAGA AAATGCGATC TCACATGGAT 
GAAAAGTTTC TGACCAAGCG CGAGCAGCAG CTGATGCAAA GGCGACACCA TGCAGAGGAG 
CTGCTCCAGT GGAAACAGCA ACTGGACAAG GAGGAGGCGG AGGTACGAAG GATGGAGAAA 
GAGGCCGTGG CCGCCTGGGA CCAGAGAAGA CCTCGGGACA AGGACCATAA AACACCTGAG 
AGCATTGGAA AAGAGATCAG TGAAAATGAA CTGCAGGCCT CTAACCACCA AAGCTTAGAG 
GCCAGATCTG ACAGTGAGAA GGAGCTTGTA AGTGAGGACA ATTGCTCCTC AATTACAACC 
GAGTCCAGTA TACACACAGA GGCTCCGGCT TCTCAGCACC CACTCAGTGC GTCCTCAGCA 
CAGCCAGCTT CAGTCGCTGA AACACCTCTG GCCTCAGTTC AGAACAGCCA TGCTACCTAC 
ACACAGGACT TTTCCACTGA GTCACAGTTA CCTGCTGTAA AGTCCTCTTC AGTCGAAGGC 
AGCCGCAGCC CTGCTGCTTC TCCATTAGAC AGCAGCAGTA AGACCAAGAT GCAACCCCGC 
TCCACCTCCC GGACACACAG TGAGGTTTTC CCTGAGGTCC TTGGAGCCGC CACAGCTATG 
CCGTCTGACC AGAGTGACAT TGAGAGTCGT ATCAAGGCAC TGAGGGAAGA ACTGAAGAAA 
CGTAAGATCA TGGCCCACCA GCTGAAAAAA GAGCAGAAGA AGCGGCACAA GGAGCGCCTG 
AAGGCACAGG AGGCAACTCT GTTGAAGAAA CTGGAGAACT ACAACAATTT TATTGAGAAA 
ACGAAGGCGG AACTGAACAA AGAGCCAGAC TCAACTCCTG AAACGACGTC ACGCATAAAT 
GATTCCAACT ACATTTTGGA GCAGTCATCT GTTAAACCCT CTTCACACAG GTCTGACGCG 
ATCAAAAGCT CCGCCTCTGA CCGGACGCTT ATTGATGCAT CATTAGATCA CAATGGCATT 
CCTCAGCCGA ACCATAGCAG AACCGCATCC AGAGATTTAC CCGAAGATTC TCCCAGTGTC 
GTCGTGTACA GCAAACCAGA GAGCGATCAT CTATCAGAGC CTCTCTCAAG ATCTTTTTTC 
AAGCCAGAGT CTGAGGAGGA GAAGGCTTTG TCTGATCACA GATCTGACAT TGAGGAAGAG 
TTGGATGTGG AAGTGAACTC CAGGTCTGAG GATGAACAGT CTCACCACCT TCTCAGACTC 
GACTCTCAGG AGAACTTAAC TGCATCTGAA CGTGTTCCGC CTTTTGCTAA GGAGGAACAT 
GAGCAGTCTC TGTCTGGCAG TTTAGATCAG GAAGAGCTGA AGAAACAAAA CAAAATACCC 
GAGGCGGAGG AAGCTGTGAC GTTAAATAAT ATTACACCCG GAACTCCTTT TTCAGCTGTT 
AACCTGGAAG CTTCACCACA TTTAAGCCGC AGCCCTTCAC CCACTGAGCA CCCTCTCTCC 
CCACACTCAG TGGGTTTCTC ACTCAATAAA GATCCACTCA TAAAGGAAGT TGAGGCTTCT 
GTTGGAACAG ACGGTTACCA GGAGGACTTT GAATCAGCTA ATTCTTCCCC TAGGGACGAC 
CATATTTCTA AGCATCAGTC TCAAACCTCC ATTCCCCACA CAGAAACTAA GCATTCAACT 
AAGGAATCAT CCAGGGCAAC CCCTCCATTT GACCATCAAG ATGAAGAAGA AGAAATACCT 
GAGGAGATGA GTCAGCATTC TGGCATCAGT GAAAAAAGCC ATCATTCAGA AAAGTTACTG 
GAACTCCAGA AGAAGGAAGA AGATATAAAA TTAGACAATA AGACAGAGTT TCCCAGCCAT 
GCACCTGCTT CTGCTCCTCC CATCGAAGAT GAAATGCTAG GTTTCCACCT TGGAGACCGG 
GTTCTTGTTG GAGGTGTTCA GCCGGGCACA CTGAGATTTA AAGGTCCGAC CAGCTTTGCC 
AATGGCTTCT GGGCTGGCGT GGAGTTGGAC AAATCTGAAG GGAGCAACAA TGGCACTTAT 
GATGGAGTTG TTTACTTTGA GTGTGAGGAA TCGCACGGCA TCTTTGCTCC TCCAGACAAA 
GTCTCCCATC TCCCAGACAA GTTTGAGCTC TATGCAGATA CCACAGAAGA TGAGGACTCT 
TTCTTCGACG ACTTATCTGA TAAAGGCGGG AAAAAACACA AAAAGATTAA AGACGATTCT 
CAAAAACGGG AAGATCCAGA AATGCGAAAT GAACAAACAT CTGACAAGGA AACTAAATCT 
AAAGATGAAA TGGTTTTGGA TGAGAATGAT CAGATTCCAC TCAAGACCAA AGCCCACCTC 
AATTCACAGC ACCAGAAAGA ACCTGAGCAT CAGATTTGTA ACGGCAACAT GAAGGACATC 
ATTTTGGACT TTACTGATGA ATCCCGCACA TTCCTCATCC CGGACAAAGA TAGAATGGAG 
GAGAAGAATG AGAAGACGAC CGCCATTATT GAGACAAACG ATGTTGACAT CGAGAAGGAC 
TTGATTCCTA CGGATGAAAT CAAGACTGAT AAGAGAGAAC AGAAGAACAG GGATCTGGTG 
GAGACATTTG CAGATGAGCT CATCAACAAC TTTATCAAAG ATTCCTTTAA GCAATGTGCT 
GAAATAAAGA GGGCTAAAGA AGGAAAGATC AGAAATGCCA ACCAGTTAAA TGGATGTTCG 
TTTGTTGAAA ATAACGACAA GAAATGGGTC TCTTCAGTGG AGCAAAAAGA CAACCTGCCC 
TTCTTTCTGC CGGCTGAGAA GGAGGAGCTG TCTTCTCCTG AACTGTGCAA CAGATCAGAG 
AGTCCAGTGT TTGGCCCCAG TGGTCAAGAG AAGCTTGCTA AGCGTCTGGC TGAGCTGGAG 
CTGAGTCGTG AGCTGCTGGA GGATCTCGGT GATGATCAGG ACTGGTTCGA TGAGGACTTT 
GGGCTCAGCT CACGGAGACA GCAGCAGAGA CTCAAACAGA AAGAAGAGCT GGTACCCCTC 
CCCTTACCTC CCAAACTACC TGAGCAGCCC GCCATGGTTG TGCCCCACTC GGCCACAGAG 
GTGGAGAAAA TGGTCCATTC TGCCACACAG GAGATCTGGG AGACCTTTCA TCTGGGAAGG 
GAGGGGGCTC TTATCCTTGA ACATCTTCCA AAGCCGACGC CTTCCCTGGA GTATTTAGGT 
AAAGAGTCCA GCAGCCAGGA CCAGGAAGCC CTGTCCATCA GCAGCTACAA AAAGGCTGTG 
TATGACCTGA CATGGGAGAT ACTGAAGGAG ATTTTTGCTG AAGACCCAAA TACTGATCAG 
CCTCAGTGGG TCAAACCACG CCAGGTCAAG TCCTCCTCCA TTCACAGAGT CAAGACGTCT 
GGAGACATCA GCAAAATTCA GGAATTTGTC ACAGAAGAAG TGTTGAAACT GTATGGTCTG 
ACACAAGATG AGAATCAAAA GACCGACTGG CAGAAAATGC TCAAATTTGG CAGGAAGAAG 
CGGGACAGAG TCGATCACAT ACTGGTCCAG GAACTCCACG AGGAAGAGGC CCAGTGGGTT 
AACTACGACG AGGACGAACT GTTTGTCAAG ATTCAGCTGG CAGACAGCAT CTTTGAGGCT 
TTGCTGAAGG ACACTGTAAA CATTTTCACA CTAATTTCTG ACAAGAGAGC CAAAAGAGGA 
GCTCTCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep350 ( XM_023339104.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023194872.1 Xiphophorus maculatus centrosomal protein 350 (cep350), transcript variant X1, mRNA.

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CloneID	OXi42336
Clone ID Related Accession (Same CDS sequence)	XM_023339104.1
Accession Version	XM_023339104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	centrosome-associated protein 350 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036451.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GCAGGAGGAC TGAAGTACCC CATACAAGAT CTGCTCAACT GCTTACTGAT 
CACAATATTG GCACAGAGCT GACAACTGCA TGGAAAACTT TGGCCCAATC CAAAGCTGCT 
CTGCGGCATA TAGAAAATCG CTTGGAGGCC ACTCCAGGAA CTGCTGCTCT TCTGGACTCC 
ATCCCCATCT CCCCCAAGAA GAAAACATCA AGTTCATCTC GCAGCAGGGA TGGGCAACAA 
GGTGATGAAT CTAGAGGGAC TTCAAAACCC AGAGGTGGAA ATCAGCAGAG TCCAGCGAAG 
AGCAGCTCAC GCAGCCCTTT GAGAAACGCC ACTCAGGACA GCAATGTACG AAAGAACAGC 
AATGTGGAGT TCAGAGAACC TCTGGCCTCA TACAGAGAGG CCACCCCGCC TTCACATCCC 
TTTTCTCAGC TGGAGGCCTA CAATCTGCAG TCCGTGCCAG GGTCTTCATA CACATCCTCT 
CTGCCTAGCT CAGATCCTCT GCCCAGCCAG CTGATCTACC AGAGAGACAC CAGGGACAAG 
CAGACTGACG GGGACTTGGA CAGCATACGA TCCTCTGCAC TGGAGAGTGC TGAGGTGCGC 
TACCTCAATG ACCAGCCGGT CTTGGACAGC CTGAAGACAG AGCACAAAGC ACAACTTGTC 
ATGGATAGTC GTGAATCAAA GAGTTTTGCT CGGACGCCCA GCTCTTCACG CCAAGGGGAG 
AGCACTCCTT CCTCAAGTCC GGGCTCAGTT ACCCAACGGT TGGAAAACCT GAGACGACAT 
CAGCCAGATG ATAAATTAGA AAAACTAAAG GAGCGCATTC GACGACAGAG GCAACATTTA 
GAGGAGACAG CAGACAAGGA AAAGTCTAAG AGTTGTCTTG AAAAGCCCAT TGTTGCGTTT 
GTTGAGGGTG ACAATGTTGG TTCAAGCAGC ATGCCAGCAG CAAAGATACG GAAAGTCACA 
GCAGCACCGC CAGCGCCTAT ATACAAAGGT TTCAACAGTG CAGAGACAAA GGTCAAAACT 
CCTGATGGTA AAGTTTGGAA AGAGGAGGAA TTCCATAACG TCAACAGAAA AGTTTACAGA 
GACATATCAC ACCAATTTTC TGAGAGCAGC AAATTCAGGC ATCAGCATCA GAGAACAGAA 
CGGTCCAAGG AGAAGAGGCC ATCCAAACCA GTCAGGAAGG TCCAAAAAGT TGCCCTGGTA 
TCTGAGCAGA ATTTTAAACC AGTGATTAGT CCTGCATCAT GGCGTGAGGG CCAAAAACTG 
GTAAAGATGG TACTAGGTCC TGCTCCAAAG CTACCCAGTG AAGAGCACAG TTCTCATCCA 
GCTGGCAGAC AGACCCGAAC AGCTTCCCAT CACCACTCAG ACCCACATTC AGAACAGAGG 
AGACGCTCTC GGCCAAACAG CACAGACAGG TCTCGCAGTG CTTTTCAGGA GCAAATGGGC 
AGCCGCCCTA CAGCCTCATA CAGTCCCTCT TCTTCAGCTC AGAACAAGAT GCCACGGGGA 
GTGAAGACAG ATGTTTTGTC AGTAGACATC CAGGGGATTC TTGATGACCT TCAGCTGGAT 
TGCAAATCAG CTGAAATAGA AGAGCGTGCT CATAAAAGAT CCCGGGGTGT CAGTGGTAGT 
AGAAGAGGAA GAGGAGGGTC AGGTTCTCGG ACGAGGGCAC CAGTCTCTGC TTGGGGATCT 
ACAGGAGTGG CGAACACCAG GTCCAGGGTC TGCCGGAGTG CTAGCCCCAC CACATGCCAG 
CCTGACAATA CTGACAAAAC AAGCAAAAGT GTCAAGAAGC GGCACTATGA CGCAGATACA 
GTTCGCCAAT ACATTGCACA ACAACAAGAG GAGAGAAAGA GGCGACAGGC AGAAGAGAAG 
AGAGCAGAAA AAGAGGAGAC AGCAAAAAGA AGTCAGAGAT TGCAGGAGCT GTACAAGAAA 
CAAAAAGAAG TAGTTAGAAC TGCGACCCTT TCCTCTGAGG CAGTTGTGGG TCCTGTTCAA 
AAGCGACTGC AGGAGACCTA CACCAGACTG CTTCTGGAGG ATGCCCGATT CAGCGAGACA 
ACACCTGAGC AACATTCTAC ACCTTTCATT CAGATGAGAC CCATGTACCA GCCTTCAGGA 
GAGTCGGACA AAGAAAACAA AAGAATCGGG GTTTCACAAA GCCCCTCAAG CAGTGACAAG 
TCTTTAAATG AGCAGCCGCC ACTGCCATTA TTCAGGAATG ATCTTGGGTT TACCTCTGTG 
CTTCAGCCTG ATCATCTGAA TCCAGCTTTT CAACCCACCA GCAGTGTACA AGTAGCTTCT 
AATGAACATC TTCTCTCTCA GCTTTTAAGG CTGGAGGCAG CATTAGCCTC TAGTAACAGA 
AAGTACACTG AACAGCCGGC TGCAGCGCCT CACCTCCTGT CCCCGACCAG AATGTCTCGT 
ATTGAAGCCC TGAAGGCAAA GGCCACATCC CTCTCAAACC GTATCGAAAC GGAGGCCCGA 
AGGTTTGCAG GACAAGGGAT CAACTATGGT GCAGCAACTT CCATGGATGT TGATACCGTT 
TTGCAACCCA GAACCTCTAG CCTTGATGTT GATCACTGGA CAGGAACCGT TGCTCATGCT 
ACAGAAAATA GCGACTTCTC CTTTAGGGCA CAGAAAGCGT TCAAAAATGC AGCTAATACT 
TCATATACTG GCATAGTTCT TCCAGGAGCA GGGAATCCGC ACGACTTAAA TGAAAAGACA 
CACACCAGTG TGACAAATCC ACATACTGGT TCTCCTGGTG TAAATGGGTT AACTTTAAAC 
ACTTACACCC AAGAAGATAG GAGGATTTTT GGTGACTTGG ACATGGAAGA GAGAACAGAA 
AAACTAGTAC AAAGAATAAA AACAAAAAAA CCGGAGAATC TGGTGTATCC GTATCAGACT 
GATCTCCACG ATTCAAGTGC TGGTTCCATT AGTGAGGGTC CTCTTCTTAG TGAAGGAAGT 
TTTTCTGAGG AAGATGGAAG TCCTCCTCAC TACACCAGTA ACCATGGGTG TAATTTAGAT 
TACTTGGAGG CTGAGGACTA CAGCGCAGGT AAAAGAAGTC ATAACAAGCG TCTGTTTGAG 
TTCCAGAGGG AGGCAGCACA GTATACAGCC CTCAGCCCGC CCTTTACACA ACTTAATGGC 
AGCAAAGCAC CATGGGAAGA GCTGAACAAA GGAAGTCCTC TGAGCGTAAT CAATATTTTC 
ACAAAAAATG TGCAAGGCCA TGTTAAAGTG AGTGAGAGAG CTTCAGGGAA TAATTCTCCA 
CTTCCTCATT CTTCACACTC CGAAAACAGC CAAGATGAAG CAGCAGTCTA TGAGGATGAC 
TTTGTTTCAT CACATAGCAG CAAAACCAGC AGCCATCTAA AGAGAGGCCC CAAGTCGCCC 
AGTCACTCAC AGCGCATTTC CCCTGGTTCA ACACCTGACT CCTCACCGCT TTCCAGGCAC 
TCCACCAGTA AAAGAGCAAC TATTTCAGAC CAGAGTGACG CAACTCTTGT AGAAGAACAG 
AGGAGTTCAC CGCTTTCAGA AACTCTCTCC TCTGACCACA AAAAGAGATC TTCAGACAGA 
AGTTTGAGTC AAGCTCAGGC CAAAAGTGCA GATTCTGACA CATTAGGAGA ACCTTCAACC 
TATTCTCCTC AAAGACCCAA ACTGGTTTCC TCGTCCTGTT CTCCAGACCT CAGTTCTTCT 
CTAACGGCAG ACTCTCTCAG TGAGCATGTG AGAAGAAGCT CTCATAATCC AAAAACATCC 
AGCAGAGCAG AGTCATGGTC CACAGGTGAG CTACAATATT CCCCTGCTGT CTTGCAGCAG 
CGCATGGCAG CAGAACTGCA GTACTTAGAG TCCATTGAAG AGTCAATCAA GCAGCTGGGA 
GACGTGGAGA AGCTGATGGG TGTGTCCATG GCACAACAGG AGAGTGCGTC ATTGACCCAA 
ATGCTCAAGG CCAAGCAGCA CCGCCATGAG CAGGAGCTCT ATGAGCTGAA GATCAAGACT 
GAAAGAGAAG CTCTCGAGAC AAAACTCCAA CTAGAAGAAA ACAGACAGAG GGTTGCCAGA 
GCTCATTTAG AGCTGCAGGA AAACTTGGCA ACATCACAAA AGCAAACTTT GGAAGGTCTC 
CAAGAGTCAA CTTCTAAGAT GATGAGCCAA CAGACTGAAG CAGCACGATA CACAGCAGAT 
GCTGCCCGAC ACATTAAGGA GATGACAGAC CTGGCACGAT CCCAGATTGC GGGGCATTTG 
ATAATCCCAC CTGTTGCCAC TGATTCTGCG ATGTTGGTCC AGCAGGAAGA ACTAAGTAGT 
TCCTACACAA AGCAGCAAGA CAAGGCAGAC TCTAGCAGAT TTAAAAGTGA GGTTTCAGAC 
CGCAGGACCA GGTCTGGAGA GCCCTCAGCT TCACAAAACG GCCTCAGTCA GTCTGACTCT 
CCATCTTTCA GAAGACCAAA CATCAGTGGA TCTCCATATG GAAGCATCCA AACCAGTCCA 
TCACATGCTC CATCTGATCA CACAAAAAGG CTGAACACAC AGTCTGGTGA GAGAAAACAG 
AAGAATGAGA GAGTTGAGGA GAGGATGAAG GCGGAAGCAG CCAGCTGCTC TATTGAGGAG 
GATGTTGTAA CAGCAGCAAA TGACTCACTC TGCAGCGAAA GTATTGCCTC TGCAGGGGAC 
GAGAAAGGAG ACACTACATC TGTGGCCACT GAGTACTCTC TAAAGTTCGA TGAGTCCATG 
ACGGAGGACG AGATAGAGGA GCGCTCTTTC CGTTCTCTGC TGCCGTCCGA AGCTCATCGT 
CGGGGAACTA TGGAGAAAAA GTCGCACCAT CAGAAGGAGG GGGACGCAGT CTGCCTCAAT 
GAAATGTTGG TCCCAGAGAC GGGGTCATGC AGTTCCTTCA AGTCTCATGA TGCACACATA 
GCCTTTTCTG GTGGGCAGGA CAGCTTTTCT CAGTTCACAA TGGAAATGGT GCGTCAGTAC 
ATGAGGGATG AAGAGGTAAG ATTGCAGCAC CAGAGCTCTC TGCTCCATCT ACGGCAGAAA 
GCGCTTAAGG AAAAGACGAG GACAGAAGTA GCCTGGCTTG AGCACCAAAA GAAAAAGCTG 
AGAGACAAAG GAGAGGATGA CAAAATGCCA CCAATCAGGA AGAAGCAGAG GGGACTTCTT 
CTGAAACTCC AACAGGAGCA AGCTGAGATC AGGAGACTCC AGGAAGCCAA CAGAGCAGCT 
AGAAAAGAAA GGCAGTTGTT GCTGAAACAG CAGGAAGAGA TTGAACGGAT GAGAAACTCA 
ACACTTAGAC TGAAGGAGCG TCTCAAGTGT GCAGAGGGAG ACATTCCCCC TGAGACGCCA 
GTGTCAGAGC CTTCAGGGTC TGAGCTCTCA CAGAGACTTC CTGGTGAAGT CCGCACCTCC 
TCTCCTTCAC TCTCCATTTC TGGAAGTGAG ACCAGCAGCA TCATGCAGAA GCTTAAGAAA 
ATGCGATCTC ACATGGATGA AAAACACTGT TCCCCTGTCC ACTACTTCTT CTCTGTGTTC 
ACGGCCCACC ACTGGGCCTC CCTCAGTGTC TGTCTTCCCA ACCTCCACCC TAAATTCCAG 
CTCTTTATCT ACAACCAGTT GGTCAGGTTT CTGACCAAGC GCGAGCAGCA GCTGATGCAA 
AGGCGACACC ATGCAGAGGA GCTGCTCCAG TGGAAACAGC AACTGGACAA GGAGGAGGCG 
GAGGTACGAA GGATGGAGAA AGAGGCCGTG GCCGCCTGGG ACCAGAGAAG ACCTCGGGAC 
AAGGACCATA AAACACCTGA GAGCATTGGA AAAGAGATCA GTGAAAATGA ACTGCAGGCC 
TCTAACCACC AAAGCTTAGA GGCCAGATCT GACAGTGAGA AGGAGCTTGT AAGTGAGGAC 
AATTGCTCCT CAATTACAAC CGAGTCCAGT ATACACACAG AGGCTCCGGC TTCTCAGCAC 
CCACTCAGTG CGTCCTCAGC ACAGCCAGCT TCAGTCGCTG AAACACCTCT GGCCTCAGTT 
CAGAACAGCC ATGCTACCTA CACACAGGAC TTTTCCACTG AGTCACAGTT ACCTGCTGTA 
AAGTCCTCTT CAGTCGAAGG CAGCCGCAGC CCTGCTGCTT CTCCATTAGA CAGCAGCAGT 
AAGACCAAGA TGCAACCCCG CTCCACCTCC CGGACACACA GTGAGGTTTT CCCTGAGGTC 
CTTGGAGCCG CCACAGCTAT GCCGTCTGAC CAGAGTGACA TTGAGAGTCG TATCAAGGCA 
CTGAGGGAAG AACTGAAGAA ACGTAAGATC ATGGCCCACC AGCTGAAAAA AGAGCAGAAG 
AAGCGGCACA AGGAGCGCCT GAAGGCACAG GAGGCAACTC TGTTGAAGAA ACTGGAGAAC 
TACAACAATT TTATTGAGAA AACGAAGGCG GAACTGAACA AAGAGCCAGA CTCAACTCCT 
GAAACGACGT CACGCATAAA TGATTCCAAC TACATTTTGG AGCAGTCATC TGTTAAACCC 
TCTTCACACA GGTCTGACGC GATCAAAAGC TCCGCCTCTG ACCGGACGCT TATTGATGCA 
TCATTAGATC ACAATGGCAT TCCTCAGCCG AACCATAGCA GAACCGCATC CAGAGATTTA 
CCCGAAGATT CTCCCAGTGT CGTCGTGTAC AGCAAACCAG AGAGCGATCA TCTATCAGAG 
CCTCTCTCAA GATCTTTTTT CAAGCCAGAG TCTGAGGAGG AGAAGGCTTT GTCTGATCAC 
AGATCTGACA TTGAGGAAGA GTTGGATGTG GAAGTGAACT CCAGGTCTGA GGATGAACAG 
TCTCACCACC TTCTCAGACT CGACTCTCAG GAGAACTTAA CTGCATCTGA ACGTGTTCCG 
CCTTTTGCTA AGGAGGAACA TGAGCAGTCT CTGTCTGGCA GTTTAGATCA GGAAGAGCTG 
AAGAAACAAA ACAAAATACC CGAGGCGGAG GAAGCTGTGA CGTTAAATAA TATTACACCC 
GGAACTCCTT TTTCAGCTGT TAACCTGGAA GCTTCACCAC ATTTAAGCCG CAGCCCTTCA 
CCCACTGAGC ACCCTCTCTC CCCACACTCA GTGGGTTTCT CACTCAATAA AGATCCACTC 
ATAAAGGAAG TTGAGGCTTC TGTTGGAACA GACGGTTACC AGGAGGACTT TGAATCAGCT 
AATTCTTCCC CTAGGGACGA CCATATTTCT AAGCATCAGT CTCAAACCTC CATTCCCCAC 
ACAGAAACTA AGCATTCAAC TAAGGAATCA TCCAGGGCAA CCCCTCCATT TGACCATCAA 
GATGAAGAAG AAGAAATACC TGAGGAGATG AGTCAGCATT CTGGCATCAG TGAAAAAAGC 
CATCATTCAG AAAAGTTACT GGAACTCCAG AAGAAGGAAG AAGATATAAA ATTAGACAAT 
AAGACAGAGT TTCCCAGCCA TGCACCTGCT TCTGCTCCTC CCATCGAAGA TGAAATGCTA 
GGTTTCCACC TTGGAGACCG GGTTCTTGTT GGAGGTGTTC AGCCGGGCAC ACTGAGATTT 
AAAGGTCCGA CCAGCTTTGC CAATGGCTTC TGGGCTGGCG TGGAGTTGGA CAAATCTGAA 
GGGAGCAACA ATGGCACTTA TGATGGAGTT GTTTACTTTG AGTGTGAGGA ATCGCACGGC 
ATCTTTGCTC CTCCAGACAA AGTCTCCCAT CTCCCAGACA AGTTTGAGCT CTATGCAGAT 
ACCACAGAAG ATGAGGACTC TTTCTTCGAC GACTTATCTG ATAAAGGCGG GAAAAAACAC 
AAAAAGATTA AAGACGATTC TCAAAAACGG GAAGATCCAG AAATGCGAAA TGAACAAACA 
TCTGACAAGG AAACTAAATC TAAAGATGAA ATGGTTTTGG ATGAGAATGA TCAGATTCCA 
CTCAAGACCA AAGCCCACCT CAATTCACAG CACCAGAAAG AACCTGAGCA TCAGATTTGT 
AACGGCAACA TGAAGGACAT CATTTTGGAC TTTACTGATG AATCCCGCAC ATTCCTCATC 
CCGGACAAAG ATAGAATGGA GGAGAAGAAT GAGAAGACGA CCGCCATTAT TGAGACAAAC 
GATGTTGACA TCGAGAAGGA CTTGATTCCT ACGGATGAAA TCAAGACTGA TAAGAGAGAA 
CAGAAGAACA GGGATCTGGT GGAGACATTT GCAGATGAGC TCATCAACAA CTTTATCAAA 
GATTCCTTTA AGCAATGTGC TGAAATAAAG AGGGCTAAAG AAGGAAAGAT CAGAAATGCC 
AACCAGTTAA ATGGATGTTC GTTTGTTGAA AATAACGACA AGAAATGGGT CTCTTCAGTG 
GAGCAAAAAG ACAACCTGCC CTTCTTTCTG CCGGCTGAGA AGGAGGAGCT GTCTTCTCCT 
GAACTGTGCA ACAGATCAGA GAGTCCAGTG TTTGGCCCCA GTGGTCAAGA GAAGCTTGCT 
AAGCGTCTGG CTGAGCTGGA GCTGAGTCGT GAGCTGCTGG AGGATCTCGG TGATGATCAG 
GACTGGTTCG ATGAGGACTT TGGGCTCAGC TCACGGAGAC AGCAGCAGAG ACTCAAACAG 
AAAGAAGAGC TGGGAGGTGA ACTCGGGAGG TCAGCCTCAT CATCAGGGCC GCACATGGGG 
GGGATGGTTT CATCGTTAGG TGGGGAATCA CAGGTGAAGA CCCCACCGAG ACCTGAGCTA 
CCCCTCCCCT TACCTCCCAA ACTACCTGAG CAGCCCGCCA TGGTTGTGCC CCACTCGGCC 
ACAGAGGTGG AGAAAATGGT CCATTCTGCC ACACAGGAGA TCTGGGAGAC CTTTCATCTG 
GGAAGGGAGG GGGCTCTTAT CCTTGAACAT CTTCCAAAGC CGACGCCTTC CCTGGAGTAT 
TTAGGTAAAG AGTCCAGCAG CCAGGACCAG GAAGCCCTGT CCATCAGCAG CTACAAAAAG 
GCTGTGTATG ACCTGACATG GGAGATACTG AAGGAGATTT TTGCTGAAGA CCCAAATACT 
GATCAGCCTC AGTGGGTCAA ACCACGCCAG GTCAAGTCCT CCTCCATTCA CAGAGTCAAG 
ACGTCTGGAG ACATCAGCAA AATTCAGGAA TTTGTCACAG AAGAAGTGTT GAAACTGTAT 
GGTCTGACAC AAGATGAGAA TCAAAAGACC GACTGGCAGA AAATGCTCAA ATTTGGCAGG 
AAGAAGCGGG ACAGAGTCGA TCACATACTG GTCCAGGAAC TCCACGAGGA AGAGGCCCAG 
TGGGTTAACT ACGACGAGGA CGAACTGTTT GTCAAGATTC AGCTGGCAGA CAGCATCTTT 
GAGGCTTTGC TGAAGGACAC TGTAAACATT TTCACACTAA TTTCTGACAA GAGAGCCAAA 
AGAGGAGCTC TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023194872.1
CDS	382..9639
Translation	MRSSRRTEVPHTRSAQLLTDHNIGTELTTAWKTLAQSKAALRHIENRLEATPGTAALLDSIPISPKKKTSSSSRSRDGQQGDESRGTSKPRGGNQQSPAKSSSRSPLRNATQDSNVRKNSNVEFREPLASYREATPPSHPFSQLEAYNLQSVPGSSYTSSLPSSDPLPSQLIYQRDTRDKQTDGDLDSIRSSALESAEVRYLNDQPVLDSLKTEHKAQLVMDSRESKSFARTPSSSRQGESTPSSSPGSVTQRLENLRRHQPDDKLEKLKERIRRQRQHLEETADKEKSKSCLEKPIVAFVEGDNVGSSSMPAAKIRKVTAAPPAPIYKGFNSAETKVKTPDGKVWKEEEFHNVNRKVYRDISHQFSESSKFRHQHQRTERSKEKRPSKPVRKVQKVALVSEQNFKPVISPASWREGQKLVKMVLGPAPKLPSEEHSSHPAGRQTRTASHHHSDPHSEQRRRSRPNSTDRSRSAFQEQMGSRPTASYSPSSSAQNKMPRGVKTDVLSVDIQGILDDLQLDCKSAEIEERAHKRSRGVSGSRRGRGGSGSRTRAPVSAWGSTGVANTRSRVCRSASPTTCQPDNTDKTSKSVKKRHYDADTVRQYIAQQQEERKRRQAEEKRAEKEETAKRSQRLQELYKKQKEVVRTATLSSEAVVGPVQKRLQETYTRLLLEDARFSETTPEQHSTPFIQMRPMYQPSGESDKENKRIGVSQSPSSSDKSLNEQPPLPLFRNDLGFTSVLQPDHLNPAFQPTSSVQVASNEHLLSQLLRLEAALASSNRKYTEQPAAAPHLLSPTRMSRIEALKAKATSLSNRIETEARRFAGQGINYGAATSMDVDTVLQPRTSSLDVDHWTGTVAHATENSDFSFRAQKAFKNAANTSYTGIVLPGAGNPHDLNEKTHTSVTNPHTGSPGVNGLTLNTYTQEDRRIFGDLDMEERTEKLVQRIKTKKPENLVYPYQTDLHDSSAGSISEGPLLSEGSFSEEDGSPPHYTSNHGCNLDYLEAEDYSAGKRSHNKRLFEFQREAAQYTALSPPFTQLNGSKAPWEELNKGSPLSVINIFTKNVQGHVKVSERASGNNSPLPHSSHSENSQDEAAVYEDDFVSSHSSKTSSHLKRGPKSPSHSQRISPGSTPDSSPLSRHSTSKRATISDQSDATLVEEQRSSPLSETLSSDHKKRSSDRSLSQAQAKSADSDTLGEPSTYSPQRPKLVSSSCSPDLSSSLTADSLSEHVRRSSHNPKTSSRAESWSTGELQYSPAVLQQRMAAELQYLESIEESIKQLGDVEKLMGVSMAQQESASLTQMLKAKQHRHEQELYELKIKTEREALETKLQLEENRQRVARAHLELQENLATSQKQTLEGLQESTSKMMSQQTEAARYTADAARHIKEMTDLARSQIAGHLIIPPVATDSAMLVQQEELSSSYTKQQDKADSSRFKSEVSDRRTRSGEPSASQNGLSQSDSPSFRRPNISGSPYGSIQTSPSHAPSDHTKRLNTQSGERKQKNERVEERMKAEAASCSIEEDVVTAANDSLCSESIASAGDEKGDTTSVATEYSLKFDESMTEDEIEERSFRSLLPSEAHRRGTMEKKSHHQKEGDAVCLNEMLVPETGSCSSFKSHDAHIAFSGGQDSFSQFTMEMVRQYMRDEEVRLQHQSSLLHLRQKALKEKTRTEVAWLEHQKKKLRDKGEDDKMPPIRKKQRGLLLKLQQEQAEIRRLQEANRAARKERQLLLKQQEEIERMRNSTLRLKERLKCAEGDIPPETPVSEPSGSELSQRLPGEVRTSSPSLSISGSETSSIMQKLKKMRSHMDEKHCSPVHYFFSVFTAHHWASLSVCLPNLHPKFQLFIYNQLVRFLTKREQQLMQRRHHAEELLQWKQQLDKEEAEVRRMEKEAVAAWDQRRPRDKDHKTPESIGKEISENELQASNHQSLEARSDSEKELVSEDNCSSITTESSIHTEAPASQHPLSASSAQPASVAETPLASVQNSHATYTQDFSTESQLPAVKSSSVEGSRSPAASPLDSSSKTKMQPRSTSRTHSEVFPEVLGAATAMPSDQSDIESRIKALREELKKRKIMAHQLKKEQKKRHKERLKAQEATLLKKLENYNNFIEKTKAELNKEPDSTPETTSRINDSNYILEQSSVKPSSHRSDAIKSSASDRTLIDASLDHNGIPQPNHSRTASRDLPEDSPSVVVYSKPESDHLSEPLSRSFFKPESEEEKALSDHRSDIEEELDVEVNSRSEDEQSHHLLRLDSQENLTASERVPPFAKEEHEQSLSGSLDQEELKKQNKIPEAEEAVTLNNITPGTPFSAVNLEASPHLSRSPSPTEHPLSPHSVGFSLNKDPLIKEVEASVGTDGYQEDFESANSSPRDDHISKHQSQTSIPHTETKHSTKESSRATPPFDHQDEEEEIPEEMSQHSGISEKSHHSEKLLELQKKEEDIKLDNKTEFPSHAPASAPPIEDEMLGFHLGDRVLVGGVQPGTLRFKGPTSFANGFWAGVELDKSEGSNNGTYDGVVYFECEESHGIFAPPDKVSHLPDKFELYADTTEDEDSFFDDLSDKGGKKHKKIKDDSQKREDPEMRNEQTSDKETKSKDEMVLDENDQIPLKTKAHLNSQHQKEPEHQICNGNMKDIILDFTDESRTFLIPDKDRMEEKNEKTTAIIETNDVDIEKDLIPTDEIKTDKREQKNRDLVETFADELINNFIKDSFKQCAEIKRAKEGKIRNANQLNGCSFVENNDKKWVSSVEQKDNLPFFLPAEKEELSSPELCNRSESPVFGPSGQEKLAKRLAELELSRELLEDLGDDQDWFDEDFGLSSRRQQQRLKQKEELGGELGRSASSSGPHMGGMVSSLGGESQVKTPPRPELPLPLPPKLPEQPAMVVPHSATEVEKMVHSATQEIWETFHLGREGALILEHLPKPTPSLEYLGKESSSQDQEALSISSYKKAVYDLTWEILKEIFAEDPNTDQPQWVKPRQVKSSSIHRVKTSGDISKIQEFVTEEVLKLYGLTQDENQKTDWQKMLKFGRKKRDRVDHILVQELHEEEAQWVNYDEDELFVKIQLADSIFEALLKDTVNIFTLISDKRAKRGALS

Target ORF information:

RefSeq Version	XM_023339104.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus centrosomal protein 350 (cep350), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023339104.1

ORF Insert Sequence:

ATGAGGAGCA GCAGGAGGAC TGAAGTACCC CATACAAGAT CTGCTCAACT GCTTACTGAT 
CACAATATTG GCACAGAGCT GACAACTGCA TGGAAAACTT TGGCCCAATC CAAAGCTGCT 
CTGCGGCATA TAGAAAATCG CTTGGAGGCC ACTCCAGGAA CTGCTGCTCT TCTGGACTCC 
ATCCCCATCT CCCCCAAGAA GAAAACATCA AGTTCATCTC GCAGCAGGGA TGGGCAACAA 
GGTGATGAAT CTAGAGGGAC TTCAAAACCC AGAGGTGGAA ATCAGCAGAG TCCAGCGAAG 
AGCAGCTCAC GCAGCCCTTT GAGAAACGCC ACTCAGGACA GCAATGTACG AAAGAACAGC 
AATGTGGAGT TCAGAGAACC TCTGGCCTCA TACAGAGAGG CCACCCCGCC TTCACATCCC 
TTTTCTCAGC TGGAGGCCTA CAATCTGCAG TCCGTGCCAG GGTCTTCATA CACATCCTCT 
CTGCCTAGCT CAGATCCTCT GCCCAGCCAG CTGATCTACC AGAGAGACAC CAGGGACAAG 
CAGACTGACG GGGACTTGGA CAGCATACGA TCCTCTGCAC TGGAGAGTGC TGAGGTGCGC 
TACCTCAATG ACCAGCCGGT CTTGGACAGC CTGAAGACAG AGCACAAAGC ACAACTTGTC 
ATGGATAGTC GTGAATCAAA GAGTTTTGCT CGGACGCCCA GCTCTTCACG CCAAGGGGAG 
AGCACTCCTT CCTCAAGTCC GGGCTCAGTT ACCCAACGGT TGGAAAACCT GAGACGACAT 
CAGCCAGATG ATAAATTAGA AAAACTAAAG GAGCGCATTC GACGACAGAG GCAACATTTA 
GAGGAGACAG CAGACAAGGA AAAGTCTAAG AGTTGTCTTG AAAAGCCCAT TGTTGCGTTT 
GTTGAGGGTG ACAATGTTGG TTCAAGCAGC ATGCCAGCAG CAAAGATACG GAAAGTCACA 
GCAGCACCGC CAGCGCCTAT ATACAAAGGT TTCAACAGTG CAGAGACAAA GGTCAAAACT 
CCTGATGGTA AAGTTTGGAA AGAGGAGGAA TTCCATAACG TCAACAGAAA AGTTTACAGA 
GACATATCAC ACCAATTTTC TGAGAGCAGC AAATTCAGGC ATCAGCATCA GAGAACAGAA 
CGGTCCAAGG AGAAGAGGCC ATCCAAACCA GTCAGGAAGG TCCAAAAAGT TGCCCTGGTA 
TCTGAGCAGA ATTTTAAACC AGTGATTAGT CCTGCATCAT GGCGTGAGGG CCAAAAACTG 
GTAAAGATGG TACTAGGTCC TGCTCCAAAG CTACCCAGTG AAGAGCACAG TTCTCATCCA 
GCTGGCAGAC AGACCCGAAC AGCTTCCCAT CACCACTCAG ACCCACATTC AGAACAGAGG 
AGACGCTCTC GGCCAAACAG CACAGACAGG TCTCGCAGTG CTTTTCAGGA GCAAATGGGC 
AGCCGCCCTA CAGCCTCATA CAGTCCCTCT TCTTCAGCTC AGAACAAGAT GCCACGGGGA 
GTGAAGACAG ATGTTTTGTC AGTAGACATC CAGGGGATTC TTGATGACCT TCAGCTGGAT 
TGCAAATCAG CTGAAATAGA AGAGCGTGCT CATAAAAGAT CCCGGGGTGT CAGTGGTAGT 
AGAAGAGGAA GAGGAGGGTC AGGTTCTCGG ACGAGGGCAC CAGTCTCTGC TTGGGGATCT 
ACAGGAGTGG CGAACACCAG GTCCAGGGTC TGCCGGAGTG CTAGCCCCAC CACATGCCAG 
CCTGACAATA CTGACAAAAC AAGCAAAAGT GTCAAGAAGC GGCACTATGA CGCAGATACA 
GTTCGCCAAT ACATTGCACA ACAACAAGAG GAGAGAAAGA GGCGACAGGC AGAAGAGAAG 
AGAGCAGAAA AAGAGGAGAC AGCAAAAAGA AGTCAGAGAT TGCAGGAGCT GTACAAGAAA 
CAAAAAGAAG TAGTTAGAAC TGCGACCCTT TCCTCTGAGG CAGTTGTGGG TCCTGTTCAA 
AAGCGACTGC AGGAGACCTA CACCAGACTG CTTCTGGAGG ATGCCCGATT CAGCGAGACA 
ACACCTGAGC AACATTCTAC ACCTTTCATT CAGATGAGAC CCATGTACCA GCCTTCAGGA 
GAGTCGGACA AAGAAAACAA AAGAATCGGG GTTTCACAAA GCCCCTCAAG CAGTGACAAG 
TCTTTAAATG AGCAGCCGCC ACTGCCATTA TTCAGGAATG ATCTTGGGTT TACCTCTGTG 
CTTCAGCCTG ATCATCTGAA TCCAGCTTTT CAACCCACCA GCAGTGTACA AGTAGCTTCT 
AATGAACATC TTCTCTCTCA GCTTTTAAGG CTGGAGGCAG CATTAGCCTC TAGTAACAGA 
AAGTACACTG AACAGCCGGC TGCAGCGCCT CACCTCCTGT CCCCGACCAG AATGTCTCGT 
ATTGAAGCCC TGAAGGCAAA GGCCACATCC CTCTCAAACC GTATCGAAAC GGAGGCCCGA 
AGGTTTGCAG GACAAGGGAT CAACTATGGT GCAGCAACTT CCATGGATGT TGATACCGTT 
TTGCAACCCA GAACCTCTAG CCTTGATGTT GATCACTGGA CAGGAACCGT TGCTCATGCT 
ACAGAAAATA GCGACTTCTC CTTTAGGGCA CAGAAAGCGT TCAAAAATGC AGCTAATACT 
TCATATACTG GCATAGTTCT TCCAGGAGCA GGGAATCCGC ACGACTTAAA TGAAAAGACA 
CACACCAGTG TGACAAATCC ACATACTGGT TCTCCTGGTG TAAATGGGTT AACTTTAAAC 
ACTTACACCC AAGAAGATAG GAGGATTTTT GGTGACTTGG ACATGGAAGA GAGAACAGAA 
AAACTAGTAC AAAGAATAAA AACAAAAAAA CCGGAGAATC TGGTGTATCC GTATCAGACT 
GATCTCCACG ATTCAAGTGC TGGTTCCATT AGTGAGGGTC CTCTTCTTAG TGAAGGAAGT 
TTTTCTGAGG AAGATGGAAG TCCTCCTCAC TACACCAGTA ACCATGGGTG TAATTTAGAT 
TACTTGGAGG CTGAGGACTA CAGCGCAGGT AAAAGAAGTC ATAACAAGCG TCTGTTTGAG 
TTCCAGAGGG AGGCAGCACA GTATACAGCC CTCAGCCCGC CCTTTACACA ACTTAATGGC 
AGCAAAGCAC CATGGGAAGA GCTGAACAAA GGAAGTCCTC TGAGCGTAAT CAATATTTTC 
ACAAAAAATG TGCAAGGCCA TGTTAAAGTG AGTGAGAGAG CTTCAGGGAA TAATTCTCCA 
CTTCCTCATT CTTCACACTC CGAAAACAGC CAAGATGAAG CAGCAGTCTA TGAGGATGAC 
TTTGTTTCAT CACATAGCAG CAAAACCAGC AGCCATCTAA AGAGAGGCCC CAAGTCGCCC 
AGTCACTCAC AGCGCATTTC CCCTGGTTCA ACACCTGACT CCTCACCGCT TTCCAGGCAC 
TCCACCAGTA AAAGAGCAAC TATTTCAGAC CAGAGTGACG CAACTCTTGT AGAAGAACAG 
AGGAGTTCAC CGCTTTCAGA AACTCTCTCC TCTGACCACA AAAAGAGATC TTCAGACAGA 
AGTTTGAGTC AAGCTCAGGC CAAAAGTGCA GATTCTGACA CATTAGGAGA ACCTTCAACC 
TATTCTCCTC AAAGACCCAA ACTGGTTTCC TCGTCCTGTT CTCCAGACCT CAGTTCTTCT 
CTAACGGCAG ACTCTCTCAG TGAGCATGTG AGAAGAAGCT CTCATAATCC AAAAACATCC 
AGCAGAGCAG AGTCATGGTC CACAGGTGAG CTACAATATT CCCCTGCTGT CTTGCAGCAG 
CGCATGGCAG CAGAACTGCA GTACTTAGAG TCCATTGAAG AGTCAATCAA GCAGCTGGGA 
GACGTGGAGA AGCTGATGGG TGTGTCCATG GCACAACAGG AGAGTGCGTC ATTGACCCAA 
ATGCTCAAGG CCAAGCAGCA CCGCCATGAG CAGGAGCTCT ATGAGCTGAA GATCAAGACT 
GAAAGAGAAG CTCTCGAGAC AAAACTCCAA CTAGAAGAAA ACAGACAGAG GGTTGCCAGA 
GCTCATTTAG AGCTGCAGGA AAACTTGGCA ACATCACAAA AGCAAACTTT GGAAGGTCTC 
CAAGAGTCAA CTTCTAAGAT GATGAGCCAA CAGACTGAAG CAGCACGATA CACAGCAGAT 
GCTGCCCGAC ACATTAAGGA GATGACAGAC CTGGCACGAT CCCAGATTGC GGGGCATTTG 
ATAATCCCAC CTGTTGCCAC TGATTCTGCG ATGTTGGTCC AGCAGGAAGA ACTAAGTAGT 
TCCTACACAA AGCAGCAAGA CAAGGCAGAC TCTAGCAGAT TTAAAAGTGA GGTTTCAGAC 
CGCAGGACCA GGTCTGGAGA GCCCTCAGCT TCACAAAACG GCCTCAGTCA GTCTGACTCT 
CCATCTTTCA GAAGACCAAA CATCAGTGGA TCTCCATATG GAAGCATCCA AACCAGTCCA 
TCACATGCTC CATCTGATCA CACAAAAAGG CTGAACACAC AGTCTGGTGA GAGAAAACAG 
AAGAATGAGA GAGTTGAGGA GAGGATGAAG GCGGAAGCAG CCAGCTGCTC TATTGAGGAG 
GATGTTGTAA CAGCAGCAAA TGACTCACTC TGCAGCGAAA GTATTGCCTC TGCAGGGGAC 
GAGAAAGGAG ACACTACATC TGTGGCCACT GAGTACTCTC TAAAGTTCGA TGAGTCCATG 
ACGGAGGACG AGATAGAGGA GCGCTCTTTC CGTTCTCTGC TGCCGTCCGA AGCTCATCGT 
CGGGGAACTA TGGAGAAAAA GTCGCACCAT CAGAAGGAGG GGGACGCAGT CTGCCTCAAT 
GAAATGTTGG TCCCAGAGAC GGGGTCATGC AGTTCCTTCA AGTCTCATGA TGCACACATA 
GCCTTTTCTG GTGGGCAGGA CAGCTTTTCT CAGTTCACAA TGGAAATGGT GCGTCAGTAC 
ATGAGGGATG AAGAGGTAAG ATTGCAGCAC CAGAGCTCTC TGCTCCATCT ACGGCAGAAA 
GCGCTTAAGG AAAAGACGAG GACAGAAGTA GCCTGGCTTG AGCACCAAAA GAAAAAGCTG 
AGAGACAAAG GAGAGGATGA CAAAATGCCA CCAATCAGGA AGAAGCAGAG GGGACTTCTT 
CTGAAACTCC AACAGGAGCA AGCTGAGATC AGGAGACTCC AGGAAGCCAA CAGAGCAGCT 
AGAAAAGAAA GGCAGTTGTT GCTGAAACAG CAGGAAGAGA TTGAACGGAT GAGAAACTCA 
ACACTTAGAC TGAAGGAGCG TCTCAAGTGT GCAGAGGGAG ACATTCCCCC TGAGACGCCA 
GTGTCAGAGC CTTCAGGGTC TGAGCTCTCA CAGAGACTTC CTGGTGAAGT CCGCACCTCC 
TCTCCTTCAC TCTCCATTTC TGGAAGTGAG ACCAGCAGCA TCATGCAGAA GCTTAAGAAA 
ATGCGATCTC ACATGGATGA AAAACACTGT TCCCCTGTCC ACTACTTCTT CTCTGTGTTC 
ACGGCCCACC ACTGGGCCTC CCTCAGTGTC TGTCTTCCCA ACCTCCACCC TAAATTCCAG 
CTCTTTATCT ACAACCAGTT GGTCAGGTTT CTGACCAAGC GCGAGCAGCA GCTGATGCAA 
AGGCGACACC ATGCAGAGGA GCTGCTCCAG TGGAAACAGC AACTGGACAA GGAGGAGGCG 
GAGGTACGAA GGATGGAGAA AGAGGCCGTG GCCGCCTGGG ACCAGAGAAG ACCTCGGGAC 
AAGGACCATA AAACACCTGA GAGCATTGGA AAAGAGATCA GTGAAAATGA ACTGCAGGCC 
TCTAACCACC AAAGCTTAGA GGCCAGATCT GACAGTGAGA AGGAGCTTGT AAGTGAGGAC 
AATTGCTCCT CAATTACAAC CGAGTCCAGT ATACACACAG AGGCTCCGGC TTCTCAGCAC 
CCACTCAGTG CGTCCTCAGC ACAGCCAGCT TCAGTCGCTG AAACACCTCT GGCCTCAGTT 
CAGAACAGCC ATGCTACCTA CACACAGGAC TTTTCCACTG AGTCACAGTT ACCTGCTGTA 
AAGTCCTCTT CAGTCGAAGG CAGCCGCAGC CCTGCTGCTT CTCCATTAGA CAGCAGCAGT 
AAGACCAAGA TGCAACCCCG CTCCACCTCC CGGACACACA GTGAGGTTTT CCCTGAGGTC 
CTTGGAGCCG CCACAGCTAT GCCGTCTGAC CAGAGTGACA TTGAGAGTCG TATCAAGGCA 
CTGAGGGAAG AACTGAAGAA ACGTAAGATC ATGGCCCACC AGCTGAAAAA AGAGCAGAAG 
AAGCGGCACA AGGAGCGCCT GAAGGCACAG GAGGCAACTC TGTTGAAGAA ACTGGAGAAC 
TACAACAATT TTATTGAGAA AACGAAGGCG GAACTGAACA AAGAGCCAGA CTCAACTCCT 
GAAACGACGT CACGCATAAA TGATTCCAAC TACATTTTGG AGCAGTCATC TGTTAAACCC 
TCTTCACACA GGTCTGACGC GATCAAAAGC TCCGCCTCTG ACCGGACGCT TATTGATGCA 
TCATTAGATC ACAATGGCAT TCCTCAGCCG AACCATAGCA GAACCGCATC CAGAGATTTA 
CCCGAAGATT CTCCCAGTGT CGTCGTGTAC AGCAAACCAG AGAGCGATCA TCTATCAGAG 
CCTCTCTCAA GATCTTTTTT CAAGCCAGAG TCTGAGGAGG AGAAGGCTTT GTCTGATCAC 
AGATCTGACA TTGAGGAAGA GTTGGATGTG GAAGTGAACT CCAGGTCTGA GGATGAACAG 
TCTCACCACC TTCTCAGACT CGACTCTCAG GAGAACTTAA CTGCATCTGA ACGTGTTCCG 
CCTTTTGCTA AGGAGGAACA TGAGCAGTCT CTGTCTGGCA GTTTAGATCA GGAAGAGCTG 
AAGAAACAAA ACAAAATACC CGAGGCGGAG GAAGCTGTGA CGTTAAATAA TATTACACCC 
GGAACTCCTT TTTCAGCTGT TAACCTGGAA GCTTCACCAC ATTTAAGCCG CAGCCCTTCA 
CCCACTGAGC ACCCTCTCTC CCCACACTCA GTGGGTTTCT CACTCAATAA AGATCCACTC 
ATAAAGGAAG TTGAGGCTTC TGTTGGAACA GACGGTTACC AGGAGGACTT TGAATCAGCT 
AATTCTTCCC CTAGGGACGA CCATATTTCT AAGCATCAGT CTCAAACCTC CATTCCCCAC 
ACAGAAACTA AGCATTCAAC TAAGGAATCA TCCAGGGCAA CCCCTCCATT TGACCATCAA 
GATGAAGAAG AAGAAATACC TGAGGAGATG AGTCAGCATT CTGGCATCAG TGAAAAAAGC 
CATCATTCAG AAAAGTTACT GGAACTCCAG AAGAAGGAAG AAGATATAAA ATTAGACAAT 
AAGACAGAGT TTCCCAGCCA TGCACCTGCT TCTGCTCCTC CCATCGAAGA TGAAATGCTA 
GGTTTCCACC TTGGAGACCG GGTTCTTGTT GGAGGTGTTC AGCCGGGCAC ACTGAGATTT 
AAAGGTCCGA CCAGCTTTGC CAATGGCTTC TGGGCTGGCG TGGAGTTGGA CAAATCTGAA 
GGGAGCAACA ATGGCACTTA TGATGGAGTT GTTTACTTTG AGTGTGAGGA ATCGCACGGC 
ATCTTTGCTC CTCCAGACAA AGTCTCCCAT CTCCCAGACA AGTTTGAGCT CTATGCAGAT 
ACCACAGAAG ATGAGGACTC TTTCTTCGAC GACTTATCTG ATAAAGGCGG GAAAAAACAC 
AAAAAGATTA AAGACGATTC TCAAAAACGG GAAGATCCAG AAATGCGAAA TGAACAAACA 
TCTGACAAGG AAACTAAATC TAAAGATGAA ATGGTTTTGG ATGAGAATGA TCAGATTCCA 
CTCAAGACCA AAGCCCACCT CAATTCACAG CACCAGAAAG AACCTGAGCA TCAGATTTGT 
AACGGCAACA TGAAGGACAT CATTTTGGAC TTTACTGATG AATCCCGCAC ATTCCTCATC 
CCGGACAAAG ATAGAATGGA GGAGAAGAAT GAGAAGACGA CCGCCATTAT TGAGACAAAC 
GATGTTGACA TCGAGAAGGA CTTGATTCCT ACGGATGAAA TCAAGACTGA TAAGAGAGAA 
CAGAAGAACA GGGATCTGGT GGAGACATTT GCAGATGAGC TCATCAACAA CTTTATCAAA 
GATTCCTTTA AGCAATGTGC TGAAATAAAG AGGGCTAAAG AAGGAAAGAT CAGAAATGCC 
AACCAGTTAA ATGGATGTTC GTTTGTTGAA AATAACGACA AGAAATGGGT CTCTTCAGTG 
GAGCAAAAAG ACAACCTGCC CTTCTTTCTG CCGGCTGAGA AGGAGGAGCT GTCTTCTCCT 
GAACTGTGCA ACAGATCAGA GAGTCCAGTG TTTGGCCCCA GTGGTCAAGA GAAGCTTGCT 
AAGCGTCTGG CTGAGCTGGA GCTGAGTCGT GAGCTGCTGG AGGATCTCGG TGATGATCAG 
GACTGGTTCG ATGAGGACTT TGGGCTCAGC TCACGGAGAC AGCAGCAGAG ACTCAAACAG 
AAAGAAGAGC TGGGAGGTGA ACTCGGGAGG TCAGCCTCAT CATCAGGGCC GCACATGGGG 
GGGATGGTTT CATCGTTAGG TGGGGAATCA CAGGTGAAGA CCCCACCGAG ACCTGAGCTA 
CCCCTCCCCT TACCTCCCAA ACTACCTGAG CAGCCCGCCA TGGTTGTGCC CCACTCGGCC 
ACAGAGGTGG AGAAAATGGT CCATTCTGCC ACACAGGAGA TCTGGGAGAC CTTTCATCTG 
GGAAGGGAGG GGGCTCTTAT CCTTGAACAT CTTCCAAAGC CGACGCCTTC CCTGGAGTAT 
TTAGGTAAAG AGTCCAGCAG CCAGGACCAG GAAGCCCTGT CCATCAGCAG CTACAAAAAG 
GCTGTGTATG ACCTGACATG GGAGATACTG AAGGAGATTT TTGCTGAAGA CCCAAATACT 
GATCAGCCTC AGTGGGTCAA ACCACGCCAG GTCAAGTCCT CCTCCATTCA CAGAGTCAAG 
ACGTCTGGAG ACATCAGCAA AATTCAGGAA TTTGTCACAG AAGAAGTGTT GAAACTGTAT 
GGTCTGACAC AAGATGAGAA TCAAAAGACC GACTGGCAGA AAATGCTCAA ATTTGGCAGG 
AAGAAGCGGG ACAGAGTCGA TCACATACTG GTCCAGGAAC TCCACGAGGA AGAGGCCCAG 
TGGGTTAACT ACGACGAGGA CGAACTGTTT GTCAAGATTC AGCTGGCAGA CAGCATCTTT 
GAGGCTTTGC TGAAGGACAC TGTAAACATT TTCACACTAA TTTCTGACAA GAGAGCCAAA 
AGAGGAGCTC TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep350 ( XM_023339105.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023194873.1 Xiphophorus maculatus centrosomal protein 350 (cep350), transcript variant X2, mRNA.

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CloneID	OXi42337
Clone ID Related Accession (Same CDS sequence)	XM_023339105.1
Accession Version	XM_023339105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	centrosome-associated protein 350 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036451.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GCAGGAGGAC TGAAGTACCC CATACAAGAT CTGCTCAACT GCTTACTGAT 
CACAATATTG GCACAGAGCT GACAACTGCA TGGAAAACTT TGGCCCAATC CAAAGCTGCT 
CTGCGGCATA TAGAAAATCG CTTGGAGGCC ACTCCAGGAA CTGCTGCTCT TCTGGACTCC 
ATCCCCATCT CCCCCAAGAA GAAAACATCA AGTTCATCTC GCAGCAGGGA TGGGCAACAA 
GGTGATGAAT CTAGAGGGAC TTCAAAACCC AGAGGTGGAA ATCAGCAGAG TCCAGCGAAG 
AGCAGCTCAC GCAGCCCTTT GAGAAACGCC ACTCAGGACA GCAATGTACG AAAGAACAGC 
AATGTGGAGT TCAGAGAACC TCTGGCCTCA TACAGAGAGG CCACCCCGCC TTCACATCCC 
TTTTCTCAGC TGGAGGCCTA CAATCTGCAG TCCGTGCCAG GGTCTTCATA CACATCCTCT 
CTGCCTAGCT CAGATCCTCT GCCCAGCCAG CTGATCTACC AGAGAGACAC CAGGGACAAG 
CAGACTGACG GGGACTTGGA CAGCATACGA TCCTCTGCAC TGGAGAGTGC TGAGGTGCGC 
TACCTCAATG ACCAGCCGGT CTTGGACAGC CTGAAGACAG AGCACAAAGC ACAACTTGTC 
ATGGATAGTC GTGAATCAAA GAGTTTTGCT CGGACGCCCA GCTCTTCACG CCAAGGGGAG 
AGCACTCCTT CCTCAAGTCC GGGCTCAGTT ACCCAACGGT TGGAAAACCT GAGACGACAT 
CAGCCAGATG ATAAATTAGA AAAACTAAAG GAGCGCATTC GACGACAGAG GCAACATTTA 
GAGGAGACAG CAGACAAGGA AAAGTCTAAG AGTTGTCTTG AAAAGCCCAT TGTTGCGTTT 
GTTGAGGGTG ACAATGTTGG TTCAAGCAGC ATGCCAGCAG CAAAGATACG GAAAGTCACA 
GCAGCACCGC CAGCGCCTAT ATACAAAGGT TTCAACAGTG CAGAGACAAA GGTCAAAACT 
CCTGATGGTA AAGTTTGGAA AGAGGAGGAA TTCCATAACG TCAACAGAAA AGTTTACAGA 
GACATATCAC ACCAATTTTC TGAGAGCAGC AAATTCAGGC ATCAGCATCA GAGAACAGAA 
CGGTCCAAGG AGAAGAGGCC ATCCAAACCA GTCAGGAAGG TCCAAAAAGT TGCCCTGGTA 
TCTGAGCAGA ATTTTAAACC AGTGATTAGT CCTGCATCAT GGCGTGAGGG CCAAAAACTG 
GTAAAGATGG TACTAGGTCC TGCTCCAAAG CTACCCAGTG AAGAGCACAG TTCTCATCCA 
GCTGGCAGAC AGACCCGAAC AGCTTCCCAT CACCACTCAG ACCCACATTC AGAACAGAGG 
AGACGCTCTC GGCCAAACAG CACAGACAGG TCTCGCAGTG CTTTTCAGGA GCAAATGGGC 
AGCCGCCCTA CAGCCTCATA CAGTCCCTCT TCTTCAGCTC AGAACAAGAT GCCACGGGGA 
GTGAAGACAG ATGTTTTGTC AGTAGACATC CAGGGGATTC TTGATGACCT TCAGCTGGAT 
TGCAAATCAG CTGAAATAGA AGAGCGTGCT CATAAAAGAT CCCGGGGTGT CAGTGGTAGT 
AGAAGAGGAA GAGGAGGGTC AGGTTCTCGG ACGAGGGCAC CAGTCTCTGC TTGGGGATCT 
ACAGGAGTGG CGAACACCAG GTCCAGGGTC TGCCGGAGTG CTAGCCCCAC CACATGCCAG 
CCTGACAATA CTGACAAAAC AAGCAAAAGT GTCAAGAAGC GGCACTATGA CGCAGATACA 
GTTCGCCAAT ACATTGCACA ACAACAAGAG GAGAGAAAGA GGCGACAGGC AGAAGAGAAG 
AGAGCAGAAA AAGAGGAGAC AGCAAAAAGA AGTCAGAGAT TGCAGGAGCT GTACAAGAAA 
CAAAAAGAAG TAGTTAGAAC TGCGACCCTT TCCTCTGAGG CAGTTGTGGG TCCTGTTCAA 
AAGCGACTGC AGGAGACCTA CACCAGACTG CTTCTGGAGG ATGCCCGATT CAGCGAGACA 
ACACCTGAGC AACATTCTAC ACCTTTCATT CAGATGAGAC CCATGTACCA GCCTTCAGGA 
GAGTCGGACA AAGAAAACAA AAGAATCGGG GTTTCACAAA GCCCCTCAAG CAGTGACAAG 
TCTTTAAATG AGCAGCCGCC ACTGCCATTA TTCAGGAATG ATCTTGGGTT TACCTCTGTG 
CTTCAGCCTG ATCATCTGAA TCCAGCTTTT CAACCCACCA GCAGTGTACA AGTAGCTTCT 
AATGAACATC TTCTCTCTCA GCTTTTAAGG CTGGAGGCAG CATTAGCCTC TAGTAACAGA 
AAGTACACTG AACAGCCGGC TGCAGCGCCT CACCTCCTGT CCCCGACCAG AATGTCTCGT 
ATTGAAGCCC TGAAGGCAAA GGCCACATCC CTCTCAAACC GTATCGAAAC GGAGGCCCGA 
AGGTTTGCAG GACAAGGGAT CAACTATGGT GCAGCAACTT CCATGGATGT TGATACCGTT 
TTGCAACCCA GAACCTCTAG CCTTGATGTT GATCACTGGA CAGGAACCGT TGCTCATGCT 
ACAGAAAATA GCGACTTCTC CTTTAGGGCA CAGAAAGCGT TCAAAAATGC AGCTAATACT 
TCATATACTG GCATAGTTCT TCCAGGAGCA GGGAATCCGC ACGACTTAAA TGAAAAGACA 
CACACCAGTG TGACAAATCC ACATACTGGT TCTCCTGGTG TAAATGGGTT AACTTTAAAC 
ACTTACACCC AAGAAGATAG GAGGATTTTT GGTGACTTGG ACATGGAAGA GAGAACAGAA 
AAACTAGTAC AAAGAATAAA AACAAAAAAA CCGGAGAATC TGGTGTATCC GTATCAGACT 
GATCTCCACG ATTCAAGTGC TGGTTCCATT AGTGAGGGTC CTCTTCTTAG TGAAGGAAGT 
TTTTCTGAGG AAGATGGAAG TCCTCCTCAC TACACCAGTA ACCATGGGTG TAATTTAGAT 
TACTTGGAGG CTGAGGACTA CAGCGCAGGT AAAAGAAGTC ATAACAAGCG TCTGTTTGAG 
TTCCAGAGGG AGGCAGCACA GTATACAGCC CTCAGCCCGC CCTTTACACA ACTTAATGGC 
AGCAAAGCAC CATGGGAAGA GCTGAACAAA GGAAGTCCTC TGAGCGTAAT CAATATTTTC 
ACAAAAAATG TGCAAGGCCA TGTTAAAGTG AGTGAGAGAG CTTCAGGGAA TAATTCTCCA 
CTTCCTCATT CTTCACACTC CGAAAACAGC CAAGATGAAG CAGCAGTCTA TGAGGATGAC 
TTTGTTTCAT CACATAGCAG CAAAACCAGC AGCCATCTAA AGAGAGGCCC CAAGTCGCCC 
AGTCACTCAC AGCGCATTTC CCCTGGTTCA ACACCTGACT CCTCACCGCT TTCCAGGCAC 
TCCACCAGTA AAAGAGCAAC TATTTCAGAC CAGAGTGACG CAACTCTTGT AGAAGAACAG 
AGGAGTTCAC CGCTTTCAGA AACTCTCTCC TCTGACCACA AAAAGAGATC TTCAGACAGA 
AGTTTGAGTC AAGCTCAGGC CAAAAGTGCA GATTCTGACA CATTAGGAGA ACCTTCAACC 
TATTCTCCTC AAAGACCCAA ACTGGTTTCC TCGTCCTGTT CTCCAGACCT CAGTTCTTCT 
CTAACGGCAG ACTCTCTCAG TGAGCATGTG AGAAGAAGCT CTCATAATCC AAAAACATCC 
AGCAGAGCAG AGTCATGGTC CACAGGTGAG CTACAATATT CCCCTGCTGT CTTGCAGCAG 
CGCATGGCAG CAGAACTGCA GTACTTAGAG TCCATTGAAG AGTCAATCAA GCAGCTGGGA 
GACGTGGAGA AGCTGATGGG TGTGTCCATG GCACAACAGG AGAGTGCGTC ATTGACCCAA 
ATGCTCAAGG CCAAGCAGCA CCGCCATGAG CAGGAGCTCT ATGAGCTGAA GATCAAGACT 
GAAAGAGAAG CTCTCGAGAC AAAACTCCAA CTAGAAGAAA ACAGACAGAG GGTTGCCAGA 
GCTCATTTAG AGCTGCAGGA AAACTTGGCA ACATCACAAA AGCAAACTTT GGAAGGTCTC 
CAAGAGTCAA CTTCTAAGAT GATGAGCCAA CAGACTGAAG CAGCACGATA CACAGCAGAT 
GCTGCCCGAC ACATTAAGGA GATGACAGAC CTGGCACGAT CCCAGATTGC GGGGCATTTG 
ATAATCCCAC CTGTTGCCAC TGATTCTGCG ATGTTGGTCC AGCAGGAAGA ACTAAGTAGT 
TCCTACACAA AGCAGCAAGA CAAGGCAGAC TCTAGCAGAT TTAAAAGTGA GGTTTCAGAC 
CGCAGGACCA GGTCTGGAGA GCCCTCAGCT TCACAAAACG GCCTCAGTCA GTCTGACTCT 
CCATCTTTCA GAAGACCAAA CATCAGTGGA TCTCCATATG GAAGCATCCA AACCAGTCCA 
TCACATGCTC CATCTGATCA CACAAAAAGG CTGAACACAC AGTCTGGTGA GAGAAAACAG 
AAGAATGAGA GAGTTGAGGA GAGGATGAAG GCGGAAGCAG CCAGCTGCTC TATTGAGGAG 
GATGTTGTAA CAGCAGCAAA TGACTCACTC TGCAGCGAAA GTATTGCCTC TGCAGGGGAC 
GAGAAAGGAG ACACTACATC TGTGGCCACT GAGTACTCTC TAAAGTTCGA TGAGTCCATG 
ACGGAGGACG AGATAGAGGA GCGCTCTTTC CGTTCTCTGC TGCCGTCCGA AGCTCATCGT 
CGGGGAACTA TGGAGAAAAA GTCGCACCAT CAGAAGGAGG GGGACGCAGT CTGCCTCAAT 
GAAATGTTGG TCCCAGAGAC GGGGTCATGC AGTTCCTTCA AGTCTCATGA TGCACACATA 
GCCTTTTCTG GTGGGCAGGA CAGCTTTTCT CAGTTCACAA TGGAAATGGT GCGTCAGTAC 
ATGAGGGATG AAGAGGTAAG ATTGCAGCAC CAGAGCTCTC TGCTCCATCT ACGGCAGAAA 
GCGCTTAAGG AAAAGACGAG GACAGAAGTA GCCTGGCTTG AGCACCAAAA GAAAAAGCTG 
AGAGACAAAG GAGAGGATGA CAAAATGCCA CCAATCAGGA AGAAGCAGAG GGGACTTCTT 
CTGAAACTCC AACAGGAGCA AGCTGAGATC AGGAGACTCC AGGAAGCCAA CAGAGCAGCT 
AGAAAAGAAA GGCAGTTGTT GCTGAAACAG CAGGAAGAGA TTGAACGGAT GAGAAACTCA 
ACACTTAGAC TGAAGGAGCG TCTCAAGTGT GCAGAGGGAG ACATTCCCCC TGAGACGCCA 
GTGTCAGAGC CTTCAGGGTC TGAGCTCTCA CAGAGACTTC CTGGTGAAGT CCGCACCTCC 
TCTCCTTCAC TCTCCATTTC TGGAAGTGAG ACCAGCAGCA TCATGCAGAA GCTTAAGAAA 
ATGCGATCTC ACATGGATGA AAAGTTTCTG ACCAAGCGCG AGCAGCAGCT GATGCAAAGG 
CGACACCATG CAGAGGAGCT GCTCCAGTGG AAACAGCAAC TGGACAAGGA GGAGGCGGAG 
GTACGAAGGA TGGAGAAAGA GGCCGTGGCC GCCTGGGACC AGAGAAGACC TCGGGACAAG 
GACCATAAAA CACCTGAGAG CATTGGAAAA GAGATCAGTG AAAATGAACT GCAGGCCTCT 
AACCACCAAA GCTTAGAGGC CAGATCTGAC AGTGAGAAGG AGCTTGTAAG TGAGGACAAT 
TGCTCCTCAA TTACAACCGA GTCCAGTATA CACACAGAGG CTCCGGCTTC TCAGCACCCA 
CTCAGTGCGT CCTCAGCACA GCCAGCTTCA GTCGCTGAAA CACCTCTGGC CTCAGTTCAG 
AACAGCCATG CTACCTACAC ACAGGACTTT TCCACTGAGT CACAGTTACC TGCTGTAAAG 
TCCTCTTCAG TCGAAGGCAG CCGCAGCCCT GCTGCTTCTC CATTAGACAG CAGCAGTAAG 
ACCAAGATGC AACCCCGCTC CACCTCCCGG ACACACAGTG AGGTTTTCCC TGAGGTCCTT 
GGAGCCGCCA CAGCTATGCC GTCTGACCAG AGTGACATTG AGAGTCGTAT CAAGGCACTG 
AGGGAAGAAC TGAAGAAACG TAAGATCATG GCCCACCAGC TGAAAAAAGA GCAGAAGAAG 
CGGCACAAGG AGCGCCTGAA GGCACAGGAG GCAACTCTGT TGAAGAAACT GGAGAACTAC 
AACAATTTTA TTGAGAAAAC GAAGGCGGAA CTGAACAAAG AGCCAGACTC AACTCCTGAA 
ACGACGTCAC GCATAAATGA TTCCAACTAC ATTTTGGAGC AGTCATCTGT TAAACCCTCT 
TCACACAGGT CTGACGCGAT CAAAAGCTCC GCCTCTGACC GGACGCTTAT TGATGCATCA 
TTAGATCACA ATGGCATTCC TCAGCCGAAC CATAGCAGAA CCGCATCCAG AGATTTACCC 
GAAGATTCTC CCAGTGTCGT CGTGTACAGC AAACCAGAGA GCGATCATCT ATCAGAGCCT 
CTCTCAAGAT CTTTTTTCAA GCCAGAGTCT GAGGAGGAGA AGGCTTTGTC TGATCACAGA 
TCTGACATTG AGGAAGAGTT GGATGTGGAA GTGAACTCCA GGTCTGAGGA TGAACAGTCT 
CACCACCTTC TCAGACTCGA CTCTCAGGAG AACTTAACTG CATCTGAACG TGTTCCGCCT 
TTTGCTAAGG AGGAACATGA GCAGTCTCTG TCTGGCAGTT TAGATCAGGA AGAGCTGAAG 
AAACAAAACA AAATACCCGA GGCGGAGGAA GCTGTGACGT TAAATAATAT TACACCCGGA 
ACTCCTTTTT CAGCTGTTAA CCTGGAAGCT TCACCACATT TAAGCCGCAG CCCTTCACCC 
ACTGAGCACC CTCTCTCCCC ACACTCAGTG GGTTTCTCAC TCAATAAAGA TCCACTCATA 
AAGGAAGTTG AGGCTTCTGT TGGAACAGAC GGTTACCAGG AGGACTTTGA ATCAGCTAAT 
TCTTCCCCTA GGGACGACCA TATTTCTAAG CATCAGTCTC AAACCTCCAT TCCCCACACA 
GAAACTAAGC ATTCAACTAA GGAATCATCC AGGGCAACCC CTCCATTTGA CCATCAAGAT 
GAAGAAGAAG AAATACCTGA GGAGATGAGT CAGCATTCTG GCATCAGTGA AAAAAGCCAT 
CATTCAGAAA AGTTACTGGA ACTCCAGAAG AAGGAAGAAG ATATAAAATT AGACAATAAG 
ACAGAGTTTC CCAGCCATGC ACCTGCTTCT GCTCCTCCCA TCGAAGATGA AATGCTAGGT 
TTCCACCTTG GAGACCGGGT TCTTGTTGGA GGTGTTCAGC CGGGCACACT GAGATTTAAA 
GGTCCGACCA GCTTTGCCAA TGGCTTCTGG GCTGGCGTGG AGTTGGACAA ATCTGAAGGG 
AGCAACAATG GCACTTATGA TGGAGTTGTT TACTTTGAGT GTGAGGAATC GCACGGCATC 
TTTGCTCCTC CAGACAAAGT CTCCCATCTC CCAGACAAGT TTGAGCTCTA TGCAGATACC 
ACAGAAGATG AGGACTCTTT CTTCGACGAC TTATCTGATA AAGGCGGGAA AAAACACAAA 
AAGATTAAAG ACGATTCTCA AAAACGGGAA GATCCAGAAA TGCGAAATGA ACAAACATCT 
GACAAGGAAA CTAAATCTAA AGATGAAATG GTTTTGGATG AGAATGATCA GATTCCACTC 
AAGACCAAAG CCCACCTCAA TTCACAGCAC CAGAAAGAAC CTGAGCATCA GATTTGTAAC 
GGCAACATGA AGGACATCAT TTTGGACTTT ACTGATGAAT CCCGCACATT CCTCATCCCG 
GACAAAGATA GAATGGAGGA GAAGAATGAG AAGACGACCG CCATTATTGA GACAAACGAT 
GTTGACATCG AGAAGGACTT GATTCCTACG GATGAAATCA AGACTGATAA GAGAGAACAG 
AAGAACAGGG ATCTGGTGGA GACATTTGCA GATGAGCTCA TCAACAACTT TATCAAAGAT 
TCCTTTAAGC AATGTGCTGA AATAAAGAGG GCTAAAGAAG GAAAGATCAG AAATGCCAAC 
CAGTTAAATG GATGTTCGTT TGTTGAAAAT AACGACAAGA AATGGGTCTC TTCAGTGGAG 
CAAAAAGACA ACCTGCCCTT CTTTCTGCCG GCTGAGAAGG AGGAGCTGTC TTCTCCTGAA 
CTGTGCAACA GATCAGAGAG TCCAGTGTTT GGCCCCAGTG GTCAAGAGAA GCTTGCTAAG 
CGTCTGGCTG AGCTGGAGCT GAGTCGTGAG CTGCTGGAGG ATCTCGGTGA TGATCAGGAC 
TGGTTCGATG AGGACTTTGG GCTCAGCTCA CGGAGACAGC AGCAGAGACT CAAACAGAAA 
GAAGAGCTGG GAGGTGAACT CGGGAGGTCA GCCTCATCAT CAGGGCCGCA CATGGGGGGG 
ATGGTTTCAT CGTTAGGTGG GGAATCACAG GTGAAGACCC CACCGAGACC TGAGCTACCC 
CTCCCCTTAC CTCCCAAACT ACCTGAGCAG CCCGCCATGG TTGTGCCCCA CTCGGCCACA 
GAGGTGGAGA AAATGGTCCA TTCTGCCACA CAGGAGATCT GGGAGACCTT TCATCTGGGA 
AGGGAGGGGG CTCTTATCCT TGAACATCTT CCAAAGCCGA CGCCTTCCCT GGAGTATTTA 
GGTAAAGAGT CCAGCAGCCA GGACCAGGAA GCCCTGTCCA TCAGCAGCTA CAAAAAGGCT 
GTGTATGACC TGACATGGGA GATACTGAAG GAGATTTTTG CTGAAGACCC AAATACTGAT 
CAGCCTCAGT GGGTCAAACC ACGCCAGGTC AAGTCCTCCT CCATTCACAG AGTCAAGACG 
TCTGGAGACA TCAGCAAAAT TCAGGAATTT GTCACAGAAG AAGTGTTGAA ACTGTATGGT 
CTGACACAAG ATGAGAATCA AAAGACCGAC TGGCAGAAAA TGCTCAAATT TGGCAGGAAG 
AAGCGGGACA GAGTCGATCA CATACTGGTC CAGGAACTCC ACGAGGAAGA GGCCCAGTGG 
GTTAACTACG ACGAGGACGA ACTGTTTGTC AAGATTCAGC TGGCAGACAG CATCTTTGAG 
GCTTTGCTGA AGGACACTGT AAACATTTTC ACACTAATTT CTGACAAGAG AGCCAAAAGA 
GGAGCTCTCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023194873.1
CDS	382..9516
Translation	MRSSRRTEVPHTRSAQLLTDHNIGTELTTAWKTLAQSKAALRHIENRLEATPGTAALLDSIPISPKKKTSSSSRSRDGQQGDESRGTSKPRGGNQQSPAKSSSRSPLRNATQDSNVRKNSNVEFREPLASYREATPPSHPFSQLEAYNLQSVPGSSYTSSLPSSDPLPSQLIYQRDTRDKQTDGDLDSIRSSALESAEVRYLNDQPVLDSLKTEHKAQLVMDSRESKSFARTPSSSRQGESTPSSSPGSVTQRLENLRRHQPDDKLEKLKERIRRQRQHLEETADKEKSKSCLEKPIVAFVEGDNVGSSSMPAAKIRKVTAAPPAPIYKGFNSAETKVKTPDGKVWKEEEFHNVNRKVYRDISHQFSESSKFRHQHQRTERSKEKRPSKPVRKVQKVALVSEQNFKPVISPASWREGQKLVKMVLGPAPKLPSEEHSSHPAGRQTRTASHHHSDPHSEQRRRSRPNSTDRSRSAFQEQMGSRPTASYSPSSSAQNKMPRGVKTDVLSVDIQGILDDLQLDCKSAEIEERAHKRSRGVSGSRRGRGGSGSRTRAPVSAWGSTGVANTRSRVCRSASPTTCQPDNTDKTSKSVKKRHYDADTVRQYIAQQQEERKRRQAEEKRAEKEETAKRSQRLQELYKKQKEVVRTATLSSEAVVGPVQKRLQETYTRLLLEDARFSETTPEQHSTPFIQMRPMYQPSGESDKENKRIGVSQSPSSSDKSLNEQPPLPLFRNDLGFTSVLQPDHLNPAFQPTSSVQVASNEHLLSQLLRLEAALASSNRKYTEQPAAAPHLLSPTRMSRIEALKAKATSLSNRIETEARRFAGQGINYGAATSMDVDTVLQPRTSSLDVDHWTGTVAHATENSDFSFRAQKAFKNAANTSYTGIVLPGAGNPHDLNEKTHTSVTNPHTGSPGVNGLTLNTYTQEDRRIFGDLDMEERTEKLVQRIKTKKPENLVYPYQTDLHDSSAGSISEGPLLSEGSFSEEDGSPPHYTSNHGCNLDYLEAEDYSAGKRSHNKRLFEFQREAAQYTALSPPFTQLNGSKAPWEELNKGSPLSVINIFTKNVQGHVKVSERASGNNSPLPHSSHSENSQDEAAVYEDDFVSSHSSKTSSHLKRGPKSPSHSQRISPGSTPDSSPLSRHSTSKRATISDQSDATLVEEQRSSPLSETLSSDHKKRSSDRSLSQAQAKSADSDTLGEPSTYSPQRPKLVSSSCSPDLSSSLTADSLSEHVRRSSHNPKTSSRAESWSTGELQYSPAVLQQRMAAELQYLESIEESIKQLGDVEKLMGVSMAQQESASLTQMLKAKQHRHEQELYELKIKTEREALETKLQLEENRQRVARAHLELQENLATSQKQTLEGLQESTSKMMSQQTEAARYTADAARHIKEMTDLARSQIAGHLIIPPVATDSAMLVQQEELSSSYTKQQDKADSSRFKSEVSDRRTRSGEPSASQNGLSQSDSPSFRRPNISGSPYGSIQTSPSHAPSDHTKRLNTQSGERKQKNERVEERMKAEAASCSIEEDVVTAANDSLCSESIASAGDEKGDTTSVATEYSLKFDESMTEDEIEERSFRSLLPSEAHRRGTMEKKSHHQKEGDAVCLNEMLVPETGSCSSFKSHDAHIAFSGGQDSFSQFTMEMVRQYMRDEEVRLQHQSSLLHLRQKALKEKTRTEVAWLEHQKKKLRDKGEDDKMPPIRKKQRGLLLKLQQEQAEIRRLQEANRAARKERQLLLKQQEEIERMRNSTLRLKERLKCAEGDIPPETPVSEPSGSELSQRLPGEVRTSSPSLSISGSETSSIMQKLKKMRSHMDEKFLTKREQQLMQRRHHAEELLQWKQQLDKEEAEVRRMEKEAVAAWDQRRPRDKDHKTPESIGKEISENELQASNHQSLEARSDSEKELVSEDNCSSITTESSIHTEAPASQHPLSASSAQPASVAETPLASVQNSHATYTQDFSTESQLPAVKSSSVEGSRSPAASPLDSSSKTKMQPRSTSRTHSEVFPEVLGAATAMPSDQSDIESRIKALREELKKRKIMAHQLKKEQKKRHKERLKAQEATLLKKLENYNNFIEKTKAELNKEPDSTPETTSRINDSNYILEQSSVKPSSHRSDAIKSSASDRTLIDASLDHNGIPQPNHSRTASRDLPEDSPSVVVYSKPESDHLSEPLSRSFFKPESEEEKALSDHRSDIEEELDVEVNSRSEDEQSHHLLRLDSQENLTASERVPPFAKEEHEQSLSGSLDQEELKKQNKIPEAEEAVTLNNITPGTPFSAVNLEASPHLSRSPSPTEHPLSPHSVGFSLNKDPLIKEVEASVGTDGYQEDFESANSSPRDDHISKHQSQTSIPHTETKHSTKESSRATPPFDHQDEEEEIPEEMSQHSGISEKSHHSEKLLELQKKEEDIKLDNKTEFPSHAPASAPPIEDEMLGFHLGDRVLVGGVQPGTLRFKGPTSFANGFWAGVELDKSEGSNNGTYDGVVYFECEESHGIFAPPDKVSHLPDKFELYADTTEDEDSFFDDLSDKGGKKHKKIKDDSQKREDPEMRNEQTSDKETKSKDEMVLDENDQIPLKTKAHLNSQHQKEPEHQICNGNMKDIILDFTDESRTFLIPDKDRMEEKNEKTTAIIETNDVDIEKDLIPTDEIKTDKREQKNRDLVETFADELINNFIKDSFKQCAEIKRAKEGKIRNANQLNGCSFVENNDKKWVSSVEQKDNLPFFLPAEKEELSSPELCNRSESPVFGPSGQEKLAKRLAELELSRELLEDLGDDQDWFDEDFGLSSRRQQQRLKQKEELGGELGRSASSSGPHMGGMVSSLGGESQVKTPPRPELPLPLPPKLPEQPAMVVPHSATEVEKMVHSATQEIWETFHLGREGALILEHLPKPTPSLEYLGKESSSQDQEALSISSYKKAVYDLTWEILKEIFAEDPNTDQPQWVKPRQVKSSSIHRVKTSGDISKIQEFVTEEVLKLYGLTQDENQKTDWQKMLKFGRKKRDRVDHILVQELHEEEAQWVNYDEDELFVKIQLADSIFEALLKDTVNIFTLISDKRAKRGALS

Target ORF information:

RefSeq Version	XM_023339105.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus centrosomal protein 350 (cep350), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023339105.1

ORF Insert Sequence:

ATGAGGAGCA GCAGGAGGAC TGAAGTACCC CATACAAGAT CTGCTCAACT GCTTACTGAT 
CACAATATTG GCACAGAGCT GACAACTGCA TGGAAAACTT TGGCCCAATC CAAAGCTGCT 
CTGCGGCATA TAGAAAATCG CTTGGAGGCC ACTCCAGGAA CTGCTGCTCT TCTGGACTCC 
ATCCCCATCT CCCCCAAGAA GAAAACATCA AGTTCATCTC GCAGCAGGGA TGGGCAACAA 
GGTGATGAAT CTAGAGGGAC TTCAAAACCC AGAGGTGGAA ATCAGCAGAG TCCAGCGAAG 
AGCAGCTCAC GCAGCCCTTT GAGAAACGCC ACTCAGGACA GCAATGTACG AAAGAACAGC 
AATGTGGAGT TCAGAGAACC TCTGGCCTCA TACAGAGAGG CCACCCCGCC TTCACATCCC 
TTTTCTCAGC TGGAGGCCTA CAATCTGCAG TCCGTGCCAG GGTCTTCATA CACATCCTCT 
CTGCCTAGCT CAGATCCTCT GCCCAGCCAG CTGATCTACC AGAGAGACAC CAGGGACAAG 
CAGACTGACG GGGACTTGGA CAGCATACGA TCCTCTGCAC TGGAGAGTGC TGAGGTGCGC 
TACCTCAATG ACCAGCCGGT CTTGGACAGC CTGAAGACAG AGCACAAAGC ACAACTTGTC 
ATGGATAGTC GTGAATCAAA GAGTTTTGCT CGGACGCCCA GCTCTTCACG CCAAGGGGAG 
AGCACTCCTT CCTCAAGTCC GGGCTCAGTT ACCCAACGGT TGGAAAACCT GAGACGACAT 
CAGCCAGATG ATAAATTAGA AAAACTAAAG GAGCGCATTC GACGACAGAG GCAACATTTA 
GAGGAGACAG CAGACAAGGA AAAGTCTAAG AGTTGTCTTG AAAAGCCCAT TGTTGCGTTT 
GTTGAGGGTG ACAATGTTGG TTCAAGCAGC ATGCCAGCAG CAAAGATACG GAAAGTCACA 
GCAGCACCGC CAGCGCCTAT ATACAAAGGT TTCAACAGTG CAGAGACAAA GGTCAAAACT 
CCTGATGGTA AAGTTTGGAA AGAGGAGGAA TTCCATAACG TCAACAGAAA AGTTTACAGA 
GACATATCAC ACCAATTTTC TGAGAGCAGC AAATTCAGGC ATCAGCATCA GAGAACAGAA 
CGGTCCAAGG AGAAGAGGCC ATCCAAACCA GTCAGGAAGG TCCAAAAAGT TGCCCTGGTA 
TCTGAGCAGA ATTTTAAACC AGTGATTAGT CCTGCATCAT GGCGTGAGGG CCAAAAACTG 
GTAAAGATGG TACTAGGTCC TGCTCCAAAG CTACCCAGTG AAGAGCACAG TTCTCATCCA 
GCTGGCAGAC AGACCCGAAC AGCTTCCCAT CACCACTCAG ACCCACATTC AGAACAGAGG 
AGACGCTCTC GGCCAAACAG CACAGACAGG TCTCGCAGTG CTTTTCAGGA GCAAATGGGC 
AGCCGCCCTA CAGCCTCATA CAGTCCCTCT TCTTCAGCTC AGAACAAGAT GCCACGGGGA 
GTGAAGACAG ATGTTTTGTC AGTAGACATC CAGGGGATTC TTGATGACCT TCAGCTGGAT 
TGCAAATCAG CTGAAATAGA AGAGCGTGCT CATAAAAGAT CCCGGGGTGT CAGTGGTAGT 
AGAAGAGGAA GAGGAGGGTC AGGTTCTCGG ACGAGGGCAC CAGTCTCTGC TTGGGGATCT 
ACAGGAGTGG CGAACACCAG GTCCAGGGTC TGCCGGAGTG CTAGCCCCAC CACATGCCAG 
CCTGACAATA CTGACAAAAC AAGCAAAAGT GTCAAGAAGC GGCACTATGA CGCAGATACA 
GTTCGCCAAT ACATTGCACA ACAACAAGAG GAGAGAAAGA GGCGACAGGC AGAAGAGAAG 
AGAGCAGAAA AAGAGGAGAC AGCAAAAAGA AGTCAGAGAT TGCAGGAGCT GTACAAGAAA 
CAAAAAGAAG TAGTTAGAAC TGCGACCCTT TCCTCTGAGG CAGTTGTGGG TCCTGTTCAA 
AAGCGACTGC AGGAGACCTA CACCAGACTG CTTCTGGAGG ATGCCCGATT CAGCGAGACA 
ACACCTGAGC AACATTCTAC ACCTTTCATT CAGATGAGAC CCATGTACCA GCCTTCAGGA 
GAGTCGGACA AAGAAAACAA AAGAATCGGG GTTTCACAAA GCCCCTCAAG CAGTGACAAG 
TCTTTAAATG AGCAGCCGCC ACTGCCATTA TTCAGGAATG ATCTTGGGTT TACCTCTGTG 
CTTCAGCCTG ATCATCTGAA TCCAGCTTTT CAACCCACCA GCAGTGTACA AGTAGCTTCT 
AATGAACATC TTCTCTCTCA GCTTTTAAGG CTGGAGGCAG CATTAGCCTC TAGTAACAGA 
AAGTACACTG AACAGCCGGC TGCAGCGCCT CACCTCCTGT CCCCGACCAG AATGTCTCGT 
ATTGAAGCCC TGAAGGCAAA GGCCACATCC CTCTCAAACC GTATCGAAAC GGAGGCCCGA 
AGGTTTGCAG GACAAGGGAT CAACTATGGT GCAGCAACTT CCATGGATGT TGATACCGTT 
TTGCAACCCA GAACCTCTAG CCTTGATGTT GATCACTGGA CAGGAACCGT TGCTCATGCT 
ACAGAAAATA GCGACTTCTC CTTTAGGGCA CAGAAAGCGT TCAAAAATGC AGCTAATACT 
TCATATACTG GCATAGTTCT TCCAGGAGCA GGGAATCCGC ACGACTTAAA TGAAAAGACA 
CACACCAGTG TGACAAATCC ACATACTGGT TCTCCTGGTG TAAATGGGTT AACTTTAAAC 
ACTTACACCC AAGAAGATAG GAGGATTTTT GGTGACTTGG ACATGGAAGA GAGAACAGAA 
AAACTAGTAC AAAGAATAAA AACAAAAAAA CCGGAGAATC TGGTGTATCC GTATCAGACT 
GATCTCCACG ATTCAAGTGC TGGTTCCATT AGTGAGGGTC CTCTTCTTAG TGAAGGAAGT 
TTTTCTGAGG AAGATGGAAG TCCTCCTCAC TACACCAGTA ACCATGGGTG TAATTTAGAT 
TACTTGGAGG CTGAGGACTA CAGCGCAGGT AAAAGAAGTC ATAACAAGCG TCTGTTTGAG 
TTCCAGAGGG AGGCAGCACA GTATACAGCC CTCAGCCCGC CCTTTACACA ACTTAATGGC 
AGCAAAGCAC CATGGGAAGA GCTGAACAAA GGAAGTCCTC TGAGCGTAAT CAATATTTTC 
ACAAAAAATG TGCAAGGCCA TGTTAAAGTG AGTGAGAGAG CTTCAGGGAA TAATTCTCCA 
CTTCCTCATT CTTCACACTC CGAAAACAGC CAAGATGAAG CAGCAGTCTA TGAGGATGAC 
TTTGTTTCAT CACATAGCAG CAAAACCAGC AGCCATCTAA AGAGAGGCCC CAAGTCGCCC 
AGTCACTCAC AGCGCATTTC CCCTGGTTCA ACACCTGACT CCTCACCGCT TTCCAGGCAC 
TCCACCAGTA AAAGAGCAAC TATTTCAGAC CAGAGTGACG CAACTCTTGT AGAAGAACAG 
AGGAGTTCAC CGCTTTCAGA AACTCTCTCC TCTGACCACA AAAAGAGATC TTCAGACAGA 
AGTTTGAGTC AAGCTCAGGC CAAAAGTGCA GATTCTGACA CATTAGGAGA ACCTTCAACC 
TATTCTCCTC AAAGACCCAA ACTGGTTTCC TCGTCCTGTT CTCCAGACCT CAGTTCTTCT 
CTAACGGCAG ACTCTCTCAG TGAGCATGTG AGAAGAAGCT CTCATAATCC AAAAACATCC 
AGCAGAGCAG AGTCATGGTC CACAGGTGAG CTACAATATT CCCCTGCTGT CTTGCAGCAG 
CGCATGGCAG CAGAACTGCA GTACTTAGAG TCCATTGAAG AGTCAATCAA GCAGCTGGGA 
GACGTGGAGA AGCTGATGGG TGTGTCCATG GCACAACAGG AGAGTGCGTC ATTGACCCAA 
ATGCTCAAGG CCAAGCAGCA CCGCCATGAG CAGGAGCTCT ATGAGCTGAA GATCAAGACT 
GAAAGAGAAG CTCTCGAGAC AAAACTCCAA CTAGAAGAAA ACAGACAGAG GGTTGCCAGA 
GCTCATTTAG AGCTGCAGGA AAACTTGGCA ACATCACAAA AGCAAACTTT GGAAGGTCTC 
CAAGAGTCAA CTTCTAAGAT GATGAGCCAA CAGACTGAAG CAGCACGATA CACAGCAGAT 
GCTGCCCGAC ACATTAAGGA GATGACAGAC CTGGCACGAT CCCAGATTGC GGGGCATTTG 
ATAATCCCAC CTGTTGCCAC TGATTCTGCG ATGTTGGTCC AGCAGGAAGA ACTAAGTAGT 
TCCTACACAA AGCAGCAAGA CAAGGCAGAC TCTAGCAGAT TTAAAAGTGA GGTTTCAGAC 
CGCAGGACCA GGTCTGGAGA GCCCTCAGCT TCACAAAACG GCCTCAGTCA GTCTGACTCT 
CCATCTTTCA GAAGACCAAA CATCAGTGGA TCTCCATATG GAAGCATCCA AACCAGTCCA 
TCACATGCTC CATCTGATCA CACAAAAAGG CTGAACACAC AGTCTGGTGA GAGAAAACAG 
AAGAATGAGA GAGTTGAGGA GAGGATGAAG GCGGAAGCAG CCAGCTGCTC TATTGAGGAG 
GATGTTGTAA CAGCAGCAAA TGACTCACTC TGCAGCGAAA GTATTGCCTC TGCAGGGGAC 
GAGAAAGGAG ACACTACATC TGTGGCCACT GAGTACTCTC TAAAGTTCGA TGAGTCCATG 
ACGGAGGACG AGATAGAGGA GCGCTCTTTC CGTTCTCTGC TGCCGTCCGA AGCTCATCGT 
CGGGGAACTA TGGAGAAAAA GTCGCACCAT CAGAAGGAGG GGGACGCAGT CTGCCTCAAT 
GAAATGTTGG TCCCAGAGAC GGGGTCATGC AGTTCCTTCA AGTCTCATGA TGCACACATA 
GCCTTTTCTG GTGGGCAGGA CAGCTTTTCT CAGTTCACAA TGGAAATGGT GCGTCAGTAC 
ATGAGGGATG AAGAGGTAAG ATTGCAGCAC CAGAGCTCTC TGCTCCATCT ACGGCAGAAA 
GCGCTTAAGG AAAAGACGAG GACAGAAGTA GCCTGGCTTG AGCACCAAAA GAAAAAGCTG 
AGAGACAAAG GAGAGGATGA CAAAATGCCA CCAATCAGGA AGAAGCAGAG GGGACTTCTT 
CTGAAACTCC AACAGGAGCA AGCTGAGATC AGGAGACTCC AGGAAGCCAA CAGAGCAGCT 
AGAAAAGAAA GGCAGTTGTT GCTGAAACAG CAGGAAGAGA TTGAACGGAT GAGAAACTCA 
ACACTTAGAC TGAAGGAGCG TCTCAAGTGT GCAGAGGGAG ACATTCCCCC TGAGACGCCA 
GTGTCAGAGC CTTCAGGGTC TGAGCTCTCA CAGAGACTTC CTGGTGAAGT CCGCACCTCC 
TCTCCTTCAC TCTCCATTTC TGGAAGTGAG ACCAGCAGCA TCATGCAGAA GCTTAAGAAA 
ATGCGATCTC ACATGGATGA AAAGTTTCTG ACCAAGCGCG AGCAGCAGCT GATGCAAAGG 
CGACACCATG CAGAGGAGCT GCTCCAGTGG AAACAGCAAC TGGACAAGGA GGAGGCGGAG 
GTACGAAGGA TGGAGAAAGA GGCCGTGGCC GCCTGGGACC AGAGAAGACC TCGGGACAAG 
GACCATAAAA CACCTGAGAG CATTGGAAAA GAGATCAGTG AAAATGAACT GCAGGCCTCT 
AACCACCAAA GCTTAGAGGC CAGATCTGAC AGTGAGAAGG AGCTTGTAAG TGAGGACAAT 
TGCTCCTCAA TTACAACCGA GTCCAGTATA CACACAGAGG CTCCGGCTTC TCAGCACCCA 
CTCAGTGCGT CCTCAGCACA GCCAGCTTCA GTCGCTGAAA CACCTCTGGC CTCAGTTCAG 
AACAGCCATG CTACCTACAC ACAGGACTTT TCCACTGAGT CACAGTTACC TGCTGTAAAG 
TCCTCTTCAG TCGAAGGCAG CCGCAGCCCT GCTGCTTCTC CATTAGACAG CAGCAGTAAG 
ACCAAGATGC AACCCCGCTC CACCTCCCGG ACACACAGTG AGGTTTTCCC TGAGGTCCTT 
GGAGCCGCCA CAGCTATGCC GTCTGACCAG AGTGACATTG AGAGTCGTAT CAAGGCACTG 
AGGGAAGAAC TGAAGAAACG TAAGATCATG GCCCACCAGC TGAAAAAAGA GCAGAAGAAG 
CGGCACAAGG AGCGCCTGAA GGCACAGGAG GCAACTCTGT TGAAGAAACT GGAGAACTAC 
AACAATTTTA TTGAGAAAAC GAAGGCGGAA CTGAACAAAG AGCCAGACTC AACTCCTGAA 
ACGACGTCAC GCATAAATGA TTCCAACTAC ATTTTGGAGC AGTCATCTGT TAAACCCTCT 
TCACACAGGT CTGACGCGAT CAAAAGCTCC GCCTCTGACC GGACGCTTAT TGATGCATCA 
TTAGATCACA ATGGCATTCC TCAGCCGAAC CATAGCAGAA CCGCATCCAG AGATTTACCC 
GAAGATTCTC CCAGTGTCGT CGTGTACAGC AAACCAGAGA GCGATCATCT ATCAGAGCCT 
CTCTCAAGAT CTTTTTTCAA GCCAGAGTCT GAGGAGGAGA AGGCTTTGTC TGATCACAGA 
TCTGACATTG AGGAAGAGTT GGATGTGGAA GTGAACTCCA GGTCTGAGGA TGAACAGTCT 
CACCACCTTC TCAGACTCGA CTCTCAGGAG AACTTAACTG CATCTGAACG TGTTCCGCCT 
TTTGCTAAGG AGGAACATGA GCAGTCTCTG TCTGGCAGTT TAGATCAGGA AGAGCTGAAG 
AAACAAAACA AAATACCCGA GGCGGAGGAA GCTGTGACGT TAAATAATAT TACACCCGGA 
ACTCCTTTTT CAGCTGTTAA CCTGGAAGCT TCACCACATT TAAGCCGCAG CCCTTCACCC 
ACTGAGCACC CTCTCTCCCC ACACTCAGTG GGTTTCTCAC TCAATAAAGA TCCACTCATA 
AAGGAAGTTG AGGCTTCTGT TGGAACAGAC GGTTACCAGG AGGACTTTGA ATCAGCTAAT 
TCTTCCCCTA GGGACGACCA TATTTCTAAG CATCAGTCTC AAACCTCCAT TCCCCACACA 
GAAACTAAGC ATTCAACTAA GGAATCATCC AGGGCAACCC CTCCATTTGA CCATCAAGAT 
GAAGAAGAAG AAATACCTGA GGAGATGAGT CAGCATTCTG GCATCAGTGA AAAAAGCCAT 
CATTCAGAAA AGTTACTGGA ACTCCAGAAG AAGGAAGAAG ATATAAAATT AGACAATAAG 
ACAGAGTTTC CCAGCCATGC ACCTGCTTCT GCTCCTCCCA TCGAAGATGA AATGCTAGGT 
TTCCACCTTG GAGACCGGGT TCTTGTTGGA GGTGTTCAGC CGGGCACACT GAGATTTAAA 
GGTCCGACCA GCTTTGCCAA TGGCTTCTGG GCTGGCGTGG AGTTGGACAA ATCTGAAGGG 
AGCAACAATG GCACTTATGA TGGAGTTGTT TACTTTGAGT GTGAGGAATC GCACGGCATC 
TTTGCTCCTC CAGACAAAGT CTCCCATCTC CCAGACAAGT TTGAGCTCTA TGCAGATACC 
ACAGAAGATG AGGACTCTTT CTTCGACGAC TTATCTGATA AAGGCGGGAA AAAACACAAA 
AAGATTAAAG ACGATTCTCA AAAACGGGAA GATCCAGAAA TGCGAAATGA ACAAACATCT 
GACAAGGAAA CTAAATCTAA AGATGAAATG GTTTTGGATG AGAATGATCA GATTCCACTC 
AAGACCAAAG CCCACCTCAA TTCACAGCAC CAGAAAGAAC CTGAGCATCA GATTTGTAAC 
GGCAACATGA AGGACATCAT TTTGGACTTT ACTGATGAAT CCCGCACATT CCTCATCCCG 
GACAAAGATA GAATGGAGGA GAAGAATGAG AAGACGACCG CCATTATTGA GACAAACGAT 
GTTGACATCG AGAAGGACTT GATTCCTACG GATGAAATCA AGACTGATAA GAGAGAACAG 
AAGAACAGGG ATCTGGTGGA GACATTTGCA GATGAGCTCA TCAACAACTT TATCAAAGAT 
TCCTTTAAGC AATGTGCTGA AATAAAGAGG GCTAAAGAAG GAAAGATCAG AAATGCCAAC 
CAGTTAAATG GATGTTCGTT TGTTGAAAAT AACGACAAGA AATGGGTCTC TTCAGTGGAG 
CAAAAAGACA ACCTGCCCTT CTTTCTGCCG GCTGAGAAGG AGGAGCTGTC TTCTCCTGAA 
CTGTGCAACA GATCAGAGAG TCCAGTGTTT GGCCCCAGTG GTCAAGAGAA GCTTGCTAAG 
CGTCTGGCTG AGCTGGAGCT GAGTCGTGAG CTGCTGGAGG ATCTCGGTGA TGATCAGGAC 
TGGTTCGATG AGGACTTTGG GCTCAGCTCA CGGAGACAGC AGCAGAGACT CAAACAGAAA 
GAAGAGCTGG GAGGTGAACT CGGGAGGTCA GCCTCATCAT CAGGGCCGCA CATGGGGGGG 
ATGGTTTCAT CGTTAGGTGG GGAATCACAG GTGAAGACCC CACCGAGACC TGAGCTACCC 
CTCCCCTTAC CTCCCAAACT ACCTGAGCAG CCCGCCATGG TTGTGCCCCA CTCGGCCACA 
GAGGTGGAGA AAATGGTCCA TTCTGCCACA CAGGAGATCT GGGAGACCTT TCATCTGGGA 
AGGGAGGGGG CTCTTATCCT TGAACATCTT CCAAAGCCGA CGCCTTCCCT GGAGTATTTA 
GGTAAAGAGT CCAGCAGCCA GGACCAGGAA GCCCTGTCCA TCAGCAGCTA CAAAAAGGCT 
GTGTATGACC TGACATGGGA GATACTGAAG GAGATTTTTG CTGAAGACCC AAATACTGAT 
CAGCCTCAGT GGGTCAAACC ACGCCAGGTC AAGTCCTCCT CCATTCACAG AGTCAAGACG 
TCTGGAGACA TCAGCAAAAT TCAGGAATTT GTCACAGAAG AAGTGTTGAA ACTGTATGGT 
CTGACACAAG ATGAGAATCA AAAGACCGAC TGGCAGAAAA TGCTCAAATT TGGCAGGAAG 
AAGCGGGACA GAGTCGATCA CATACTGGTC CAGGAACTCC ACGAGGAAGA GGCCCAGTGG 
GTTAACTACG ACGAGGACGA ACTGTTTGTC AAGATTCAGC TGGCAGACAG CATCTTTGAG 
GCTTTGCTGA AGGACACTGT AAACATTTTC ACACTAATTT CTGACAAGAG AGCCAAAAGA 
GGAGCTCTCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cep350 cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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