Tc00.1047053510121.170 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053510121.170
Entrez Gene ID	3548378
Full Name	calpain cysteine peptidase
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_811385.1	XP_816478.1	calpain cysteine peptidase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053510121.170 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053510121.170 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb10988	XM_811385.1 Latest version!	Trypanosoma cruzi strain CL Brener calpain cysteine peptidase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053510121.170 ( XM_811385.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_816478.1 Trypanosoma cruzi strain CL Brener calpain cysteine peptidase partial mRNA.

Price & Availability↑

CloneID	OTb10988
Clone ID Related Accession (Same CDS sequence)	XM_811385.1
Accession Version	XM_811385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	calpain cysteine peptidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849426). COMPLETENESS: incomplete on both ends.

ATGACAACAC TCGAAGACCT CTACGCATCG TACTGCCGCC GACGGGGGTG TAGGCCAAAC 
AGCTTCTTGT CCAGGTACCT GCAGGAGGAG TTCACGCGAC ACAGCCAGCA GCGAGTGCTG 
GAGAGCGTCG ACCTCTCGGA TAACTACGTG GGCGCGAAGG GCATCATTCC CGTGCTGGAT 
CTCGTCAAGA ATGTGAAGAC CGTGAGGAGG CTGGATGTCC GGAAAAACAT GCTCGAGCAC 
GAGCAACTGG AGCACCTTGT CTACTGCCTT GCCCTGCATC CATCAATAGA GGAGGTGGAC 
GTTTCAGGCA ACGCTCTCGA TGATGGCAGC GTCGACATCA TTTTGAAACT TCTCCAGGCG 
AATGAGAGCA TCACCGTCTT CCGTGTGGAC GGGAATCATT TTGCGTCTTC CTCCCTGTCG 
CTGATCAATG ACCATCTGGA GAGGAACAAG GAACGCAAAG CTGAACGGCA AAAGGAAATG 
ACACCCGTAT ACACTGGCAA ATGGTTCCGT GCGCAGGTGC GGGGATCTCT AGCGGAAGAA 
ACATCGGGTG GCCATGTACA CTTCTCTACC TGGTGGAAGA ACCAACAGTA CATTATGCGA 
GTATCACGAC GAGCCCAGGT CCGTGTCGTC ATGGATGTCG ATGATCCAAA GGCCGCACGG 
CAGGCTGGGT TCTTTGTCTT TTATTCCGAT GGTACACGCA AAGTAATTGT GGCCGATGCG 
GATCATATTG CTGCGGAAAG TAATGTGGAC CACAGTCATT GTTTTGTGAC GTTGTTGGTT 
GAGGAGCACG CTGCTTATTC CATCATGCCC TTTACATTTC ATCCAGAACG AAGCATGTCA 
TTTACGCTGA TGGTGGAATT ATGCCAGGAG AGCTTGAAAA GCTCAGAGGG TTGGGTTACA 
TTGGAGCCTG TGGATTCTGC GCTTGACTGG CTTGTGTATG TTATGCACGG CGAGTGGACA 
AGCGCCTCAG CCGGTGGTTC CCCAACGCAG CATTTGTGGT GCCGCAATCC CATGATACAT 
GTACAATACG CCGGTTCCAT TCAGTCATAC CGAATGGCAT CTCCTGCCAC CGTGTTTGTG 
CAGCTTAGCA AGAGCATTGA TGCGGATGAA AACGACGATA GGCATATTGG ATTTGATGTT 
GTTACACTGG ATACGACGGG GAACAACAAA CCACCCATCT GCTGCAAAGA AGAATCCCGC 
AAGTGTACGT GCCCTCACGA GCACAGAACG ACGGTTACAG CTTCCTTTGT GATTATGTGT 
GCTGCTCTTG ACTTGTTTAT TGTCCCGAGC ACGGCGATAC CTGGGGAGTT GGGTTCCTAC 
ACTGTGACTG TCTTTTCTTC TGTCCCTCTC CGACTGACCA CATCTGCGTT TCCTCACGGA 
TGGAACTACC GAGCGGTGAA AGGCGTCTGG GATGAAGACA ACTGCGGAGG GAGTCGGGAA 
CGCAGCATGT CATGGAAGTG CAATCCCTCT CTTGCACTCC ACTTTGACGC CCATCAATCC 
CCTCCAGATT TAACTTTATT CTTGGAGGAA TCATTGCCCC GTCCGAAGAG CCCTGTCCGC 
CGTGGCAGCG CCGACTCTTG CGGCAACTTA ATGGATTCCC TCTCAGCAGT GGAGGAGACT 
CAGAAAAAAA AGGAACTAAA AGAGTTTCTA CATCGGCACC AACTGAGTCA TTTGGAGGGA 
TGTATCTCCC TTGTCGAGGC CTGCCCACCC ATGTACCGTT TGCTTTATTC CTCTGAATAC 
TCCCGGGGCT CGGTCGTGTC TCTTGTGGTG CCTGCTGTCA GCGAGGAATT TTTTTTGCTG 
GCTGCAACGC GCCACGCTGG TCAACTGGGT AATTTTATAC TTCACATCTT CTCGTCGCGT 
CCGTTTGTGA CTGACGAACT GAAGACGTTG GCGTTGCGGG AACGGGAGTA TCAATTGCTG 
CAGTGCGCTC AGGAATCCCA GGCTCGTCAG GCAGCGAATT CGGTATTGGC AAAAAAACGG 
TCTGGTCCCA AAGACTCTGA GGATGCGGTT CTTGCGCACA ACGAGATCCT TCTTCGTTGC 
ATGGTGACTG GAGAGAAGTA CGTGGATCGG GACTTTCCAC GTGGTGGATC CTCCTTGTGG 
ATCGATCCGG CGAAAAAGAC ACCGGCGTGG TGTGGGAAAG AGATTTATTG GAAGCGACCG 
ACGGAGCTGG TGGAGGAAGT GACATTTCTC CGGGAGTGGA AGTGTGACTG CCCCTTTCCT 
TTCTCGAGAC GGGAGTGGTT TGCCTCCGTC ACATATGCGA TTGCTACGAA ACCGCTCTGG 
TTACAGAATC TAACTGCTGG ATACAACGTC ACGGAGGGGT TAGCGCAGTT CCGTTTCTTC 
AAGAGCGGCC AATGGACTTT GGTGACCATC GACGATTACC TACCATTTGA CAGTACCATG 
GAACTGTGCA TGGGACGCCC CTCAAGAGAC AACACAGACT TTTTCTTCCC CCTTCTGGAG 
AAAGCGTACG CAAAGCACCA TCGCTGCTAC GAGGCACTAG AGCTAAAAGT CACCCCGGAA 
CTGAGCATCG TGGACGTGAT GTGTCATGGC CTCATGGATC TTTCAGGGTG TGCCCCGGTT 
CATTTCCCCC TGAGGGGCTC TGTGGAGATG TCAGCGGAGC AACAAAACAT CCTATGGATG 
AAGCTTAAAA ACGCCATTCA GCAGGACGTT CTTTTCACCT TTTTATTGCG CGGCGACAGT 
GCTGAGGCGT CTGAGCGAAT CAGTTTGGGC ATTTTGAGTG ACCACTTGTA CCCCGCACTA 
GATGCACGTT TTGTGGAGGG GCAAAGGCTG GTGAAGTTGC GTCACTGGGG GCAGGTGGGG 
GAAGTCCGCT GGGGTGGAAA GTGGCGGGCG ATGAGCACAC GATGGACCAC GATCCTTCGC 
GATCTTCTGA AGTTTGATGA AGACGACAGG GAAACGTTTT GGATGAGTTT GGATGAGGTG 
TTTTTTTACT TCACGGACCT CATCATGACA GCGGGGACAA AGCACACGTC ATGGGTATCG 
GCGGACTTTG CCGATTGCCC GAAAGAGTGT GGCACTCCAG TAATGGAGGG GGCCCAGTTC 
ACTCTTCGGC TTGGAGATCT CCCACCCGAC CTGAATAAGA CACAGATTTC GTTAGGCCTC 
CACCAGCCAG ATGCACGCGC CAGAGTCATA CGACAAAAGA ATGCTCTGGC GGCATACCGC 
ACAGCCATCG GCCTGGCAGT TGTGGCCACG GAAGACAACA CCGTATGGCT AAAGGAGGTG 
CGGGAAGCCG AAGTGGTGAA ATGTTTAGAG CCGTGTAAAT GTCGAGATGT CATGTGTTCT 
CTAAATATCG ACATGGATAA TGTTAAGGGG AGCAAACGCC TGACGCTTAT TGCGTTTAGG 
GAAGATCAGA AGGCTGCGAA TGTACCATTT CTGTTATCTG CGTGGTCTGA TAATTGCGAG 
GTTGCGTTAA CTCCGATCAC GCGAGATATC AAAACGACAG TAAGTGGGGA GTGGCCGATT 
GGATACCCTG TTGGGTCACC CTCATCGTCT TTCTGGAGAG ACTGTCCACA GTACTTTGTA 
TTCCCCAGTG AATCCACGGA GTTTCTTTTT GTTCTTCGTC AGGATTTACC AGTGGGGGAA 
TTACCGAAGC CAATTGGGTT CACAGTTCAC CGTGAGATGA CGTGTCGAAG TTATCTGGAG 
TACAATCCAG ACACAGTGGT TCTTTATGTA CAGGCGGTAG CGTCTGCATG TGTTGAAGGC 
ACAGTAAGAC TTCTCGGAAT GAAGGAGCGA AGAGGAATGC CGTACATTAT TGTGCCCTTC 
TGCACCGAGG CCACACCTGG GGGTAAATTT TGGATCGATG CCATCGCCAA TCGTTCCTCG 
AGGTTTTGCT GCATAGATCC AAGACTTGAC TGGTACCGTG ACAGAAAGTC TGTTTCTTTT 
ACCTTGGCTG ACGGTAGTTT TGGTGGGTCT CCGCGTTTTT CGTCATGGCG CAGCAGTCCG 
CAGTTGGCCC TGAACTTCCC CGTTGGGGGT CAGGGAAGAC TGTTTGTTGT TGTCCGGAAT 
GATGATTTGA GCGATAACCG GACGGAACTC GGCATGATGC TCCTGCGTGG CGATAATCAG 
TGGGAAAATG GACTGCGGCG AAAGCTGTTT ATTTCCTCTG GTGACATTGT GGCTCGCTCG 
GAAGAAAAAA TTGGGGAAAC CGTCATTGAC TGCAATGTCG ATGTGCAGCC TGAGTGCACC 
CTCATTCTCG TCGTGTACGC CTCCATGCCG TATCGTGAGG CGGCTGTGAC GGTCGCCCTT 
TATTCCGCCT CCGCCGTGGA AGTGGAGCCC GTGAAGGAGT GGGCGCAGGT CGCTGTGGCG 
GAGGGCAGCT GGGAGCTCGG CTACACGGCC GGCGGGGGAT CCGATCAGTT TGGCAGCTGG 
ATCAACAACC CGTTTGTGGC GCTAAATACG TATCGGCGGA CGCAAATCGT GGCGCTTCTG 
CTGCAGTATC CGCAGGGGCC GGACAAGCCT CTTGTGAAGC GTGCGGGAAA GAAAAAGGCA 
TTCTTGCCCC CCATTATCAT CAATCCAAAC AATAGAATGA TGATTGCGCT GGACCTGAAC 
GTGCAGAACA GCGAACTTAC ACCCATTGCC TCCACTCCCT ACACTTACAA CAGTGAGGTG 
ACACTTGTGG CGCACGTTCC TGCGGCAGAC TCGTTGCCAT TTCTTTTCAT TCCCCACACA 
AAACTACCCG AAGGGAATGG CGAGTTTAAA CTCTTTGTGT ACGCGGACTC CCCCATTGAA 
CTCTATACGC TTGAAAAGAA ACGCTTGCCT TACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_816478.1
CDS	1..4779
Translation	MTTLEDLYASYCRRRGCRPNSFLSRYLQEEFTRHSQQRVLESVDLSDNYVGAKGIIPVLDLVKNVKTVRRLDVRKNMLEHEQLEHLVYCLALHPSIEEVDVSGNALDDGSVDIILKLLQANESITVFRVDGNHFASSSLSLINDHLERNKERKAERQKEMTPVYTGKWFRAQVRGSLAEETSGGHVHFSTWWKNQQYIMRVSRRAQVRVVMDVDDPKAARQAGFFVFYSDGTRKVIVADADHIAAESNVDHSHCFVTLLVEEHAAYSIMPFTFHPERSMSFTLMVELCQESLKSSEGWVTLEPVDSALDWLVYVMHGEWTSASAGGSPTQHLWCRNPMIHVQYAGSIQSYRMASPATVFVQLSKSIDADENDDRHIGFDVVTLDTTGNNKPPICCKEESRKCTCPHEHRTTVTASFVIMCAALDLFIVPSTAIPGELGSYTVTVFSSVPLRLTTSAFPHGWNYRAVKGVWDEDNCGGSRERSMSWKCNPSLALHFDAHQSPPDLTLFLEESLPRPKSPVRRGSADSCGNLMDSLSAVEETQKKKELKEFLHRHQLSHLEGCISLVEACPPMYRLLYSSEYSRGSVVSLVVPAVSEEFFLLAATRHAGQLGNFILHIFSSRPFVTDELKTLALREREYQLLQCAQESQARQAANSVLAKKRSGPKDSEDAVLAHNEILLRCMVTGEKYVDRDFPRGGSSLWIDPAKKTPAWCGKEIYWKRPTELVEEVTFLREWKCDCPFPFSRREWFASVTYAIATKPLWLQNLTAGYNVTEGLAQFRFFKSGQWTLVTIDDYLPFDSTMELCMGRPSRDNTDFFFPLLEKAYAKHHRCYEALELKVTPELSIVDVMCHGLMDLSGCAPVHFPLRGSVEMSAEQQNILWMKLKNAIQQDVLFTFLLRGDSAEASERISLGILSDHLYPALDARFVEGQRLVKLRHWGQVGEVRWGGKWRAMSTRWTTILRDLLKFDEDDRETFWMSLDEVFFYFTDLIMTAGTKHTSWVSADFADCPKECGTPVMEGAQFTLRLGDLPPDLNKTQISLGLHQPDARARVIRQKNALAAYRTAIGLAVVATEDNTVWLKEVREAEVVKCLEPCKCRDVMCSLNIDMDNVKGSKRLTLIAFREDQKAANVPFLLSAWSDNCEVALTPITRDIKTTVSGEWPIGYPVGSPSSSFWRDCPQYFVFPSESTEFLFVLRQDLPVGELPKPIGFTVHREMTCRSYLEYNPDTVVLYVQAVASACVEGTVRLLGMKERRGMPYIIVPFCTEATPGGKFWIDAIANRSSRFCCIDPRLDWYRDRKSVSFTLADGSFGGSPRFSSWRSSPQLALNFPVGGQGRLFVVVRNDDLSDNRTELGMMLLRGDNQWENGLRRKLFISSGDIVARSEEKIGETVIDCNVDVQPECTLILVVYASMPYREAAVTVALYSASAVEVEPVKEWAQVAVAEGSWELGYTAGGGSDQFGSWINNPFVALNTYRRTQIVALLLQYPQGPDKPLVKRAGKKKAFLPPIIINPNNRMMIALDLNVQNSELTPIASTPYTYNSEVTLVAHVPAADSLPFLFIPHTKLPEGNGEFKLFVYADSPIELYTLEKKRLPYV

Target ORF information:

RefSeq Version	XM_811385.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener calpain cysteine peptidase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_811385.1

ORF Insert Sequence:

ATGACAACAC TCGAAGACCT CTACGCATCG TACTGCCGCC GACGGGGGTG TAGGCCAAAC 
AGCTTCTTGT CCAGGTACCT GCAGGAGGAG TTCACGCGAC ACAGCCAGCA GCGAGTGCTG 
GAGAGCGTCG ACCTCTCGGA TAACTACGTG GGCGCGAAGG GCATCATTCC CGTGCTGGAT 
CTCGTCAAGA ATGTGAAGAC CGTGAGGAGG CTGGATGTCC GGAAAAACAT GCTCGAGCAC 
GAGCAACTGG AGCACCTTGT CTACTGCCTT GCCCTGCATC CATCAATAGA GGAGGTGGAC 
GTTTCAGGCA ACGCTCTCGA TGATGGCAGC GTCGACATCA TTTTGAAACT TCTCCAGGCG 
AATGAGAGCA TCACCGTCTT CCGTGTGGAC GGGAATCATT TTGCGTCTTC CTCCCTGTCG 
CTGATCAATG ACCATCTGGA GAGGAACAAG GAACGCAAAG CTGAACGGCA AAAGGAAATG 
ACACCCGTAT ACACTGGCAA ATGGTTCCGT GCGCAGGTGC GGGGATCTCT AGCGGAAGAA 
ACATCGGGTG GCCATGTACA CTTCTCTACC TGGTGGAAGA ACCAACAGTA CATTATGCGA 
GTATCACGAC GAGCCCAGGT CCGTGTCGTC ATGGATGTCG ATGATCCAAA GGCCGCACGG 
CAGGCTGGGT TCTTTGTCTT TTATTCCGAT GGTACACGCA AAGTAATTGT GGCCGATGCG 
GATCATATTG CTGCGGAAAG TAATGTGGAC CACAGTCATT GTTTTGTGAC GTTGTTGGTT 
GAGGAGCACG CTGCTTATTC CATCATGCCC TTTACATTTC ATCCAGAACG AAGCATGTCA 
TTTACGCTGA TGGTGGAATT ATGCCAGGAG AGCTTGAAAA GCTCAGAGGG TTGGGTTACA 
TTGGAGCCTG TGGATTCTGC GCTTGACTGG CTTGTGTATG TTATGCACGG CGAGTGGACA 
AGCGCCTCAG CCGGTGGTTC CCCAACGCAG CATTTGTGGT GCCGCAATCC CATGATACAT 
GTACAATACG CCGGTTCCAT TCAGTCATAC CGAATGGCAT CTCCTGCCAC CGTGTTTGTG 
CAGCTTAGCA AGAGCATTGA TGCGGATGAA AACGACGATA GGCATATTGG ATTTGATGTT 
GTTACACTGG ATACGACGGG GAACAACAAA CCACCCATCT GCTGCAAAGA AGAATCCCGC 
AAGTGTACGT GCCCTCACGA GCACAGAACG ACGGTTACAG CTTCCTTTGT GATTATGTGT 
GCTGCTCTTG ACTTGTTTAT TGTCCCGAGC ACGGCGATAC CTGGGGAGTT GGGTTCCTAC 
ACTGTGACTG TCTTTTCTTC TGTCCCTCTC CGACTGACCA CATCTGCGTT TCCTCACGGA 
TGGAACTACC GAGCGGTGAA AGGCGTCTGG GATGAAGACA ACTGCGGAGG GAGTCGGGAA 
CGCAGCATGT CATGGAAGTG CAATCCCTCT CTTGCACTCC ACTTTGACGC CCATCAATCC 
CCTCCAGATT TAACTTTATT CTTGGAGGAA TCATTGCCCC GTCCGAAGAG CCCTGTCCGC 
CGTGGCAGCG CCGACTCTTG CGGCAACTTA ATGGATTCCC TCTCAGCAGT GGAGGAGACT 
CAGAAAAAAA AGGAACTAAA AGAGTTTCTA CATCGGCACC AACTGAGTCA TTTGGAGGGA 
TGTATCTCCC TTGTCGAGGC CTGCCCACCC ATGTACCGTT TGCTTTATTC CTCTGAATAC 
TCCCGGGGCT CGGTCGTGTC TCTTGTGGTG CCTGCTGTCA GCGAGGAATT TTTTTTGCTG 
GCTGCAACGC GCCACGCTGG TCAACTGGGT AATTTTATAC TTCACATCTT CTCGTCGCGT 
CCGTTTGTGA CTGACGAACT GAAGACGTTG GCGTTGCGGG AACGGGAGTA TCAATTGCTG 
CAGTGCGCTC AGGAATCCCA GGCTCGTCAG GCAGCGAATT CGGTATTGGC AAAAAAACGG 
TCTGGTCCCA AAGACTCTGA GGATGCGGTT CTTGCGCACA ACGAGATCCT TCTTCGTTGC 
ATGGTGACTG GAGAGAAGTA CGTGGATCGG GACTTTCCAC GTGGTGGATC CTCCTTGTGG 
ATCGATCCGG CGAAAAAGAC ACCGGCGTGG TGTGGGAAAG AGATTTATTG GAAGCGACCG 
ACGGAGCTGG TGGAGGAAGT GACATTTCTC CGGGAGTGGA AGTGTGACTG CCCCTTTCCT 
TTCTCGAGAC GGGAGTGGTT TGCCTCCGTC ACATATGCGA TTGCTACGAA ACCGCTCTGG 
TTACAGAATC TAACTGCTGG ATACAACGTC ACGGAGGGGT TAGCGCAGTT CCGTTTCTTC 
AAGAGCGGCC AATGGACTTT GGTGACCATC GACGATTACC TACCATTTGA CAGTACCATG 
GAACTGTGCA TGGGACGCCC CTCAAGAGAC AACACAGACT TTTTCTTCCC CCTTCTGGAG 
AAAGCGTACG CAAAGCACCA TCGCTGCTAC GAGGCACTAG AGCTAAAAGT CACCCCGGAA 
CTGAGCATCG TGGACGTGAT GTGTCATGGC CTCATGGATC TTTCAGGGTG TGCCCCGGTT 
CATTTCCCCC TGAGGGGCTC TGTGGAGATG TCAGCGGAGC AACAAAACAT CCTATGGATG 
AAGCTTAAAA ACGCCATTCA GCAGGACGTT CTTTTCACCT TTTTATTGCG CGGCGACAGT 
GCTGAGGCGT CTGAGCGAAT CAGTTTGGGC ATTTTGAGTG ACCACTTGTA CCCCGCACTA 
GATGCACGTT TTGTGGAGGG GCAAAGGCTG GTGAAGTTGC GTCACTGGGG GCAGGTGGGG 
GAAGTCCGCT GGGGTGGAAA GTGGCGGGCG ATGAGCACAC GATGGACCAC GATCCTTCGC 
GATCTTCTGA AGTTTGATGA AGACGACAGG GAAACGTTTT GGATGAGTTT GGATGAGGTG 
TTTTTTTACT TCACGGACCT CATCATGACA GCGGGGACAA AGCACACGTC ATGGGTATCG 
GCGGACTTTG CCGATTGCCC GAAAGAGTGT GGCACTCCAG TAATGGAGGG GGCCCAGTTC 
ACTCTTCGGC TTGGAGATCT CCCACCCGAC CTGAATAAGA CACAGATTTC GTTAGGCCTC 
CACCAGCCAG ATGCACGCGC CAGAGTCATA CGACAAAAGA ATGCTCTGGC GGCATACCGC 
ACAGCCATCG GCCTGGCAGT TGTGGCCACG GAAGACAACA CCGTATGGCT AAAGGAGGTG 
CGGGAAGCCG AAGTGGTGAA ATGTTTAGAG CCGTGTAAAT GTCGAGATGT CATGTGTTCT 
CTAAATATCG ACATGGATAA TGTTAAGGGG AGCAAACGCC TGACGCTTAT TGCGTTTAGG 
GAAGATCAGA AGGCTGCGAA TGTACCATTT CTGTTATCTG CGTGGTCTGA TAATTGCGAG 
GTTGCGTTAA CTCCGATCAC GCGAGATATC AAAACGACAG TAAGTGGGGA GTGGCCGATT 
GGATACCCTG TTGGGTCACC CTCATCGTCT TTCTGGAGAG ACTGTCCACA GTACTTTGTA 
TTCCCCAGTG AATCCACGGA GTTTCTTTTT GTTCTTCGTC AGGATTTACC AGTGGGGGAA 
TTACCGAAGC CAATTGGGTT CACAGTTCAC CGTGAGATGA CGTGTCGAAG TTATCTGGAG 
TACAATCCAG ACACAGTGGT TCTTTATGTA CAGGCGGTAG CGTCTGCATG TGTTGAAGGC 
ACAGTAAGAC TTCTCGGAAT GAAGGAGCGA AGAGGAATGC CGTACATTAT TGTGCCCTTC 
TGCACCGAGG CCACACCTGG GGGTAAATTT TGGATCGATG CCATCGCCAA TCGTTCCTCG 
AGGTTTTGCT GCATAGATCC AAGACTTGAC TGGTACCGTG ACAGAAAGTC TGTTTCTTTT 
ACCTTGGCTG ACGGTAGTTT TGGTGGGTCT CCGCGTTTTT CGTCATGGCG CAGCAGTCCG 
CAGTTGGCCC TGAACTTCCC CGTTGGGGGT CAGGGAAGAC TGTTTGTTGT TGTCCGGAAT 
GATGATTTGA GCGATAACCG GACGGAACTC GGCATGATGC TCCTGCGTGG CGATAATCAG 
TGGGAAAATG GACTGCGGCG AAAGCTGTTT ATTTCCTCTG GTGACATTGT GGCTCGCTCG 
GAAGAAAAAA TTGGGGAAAC CGTCATTGAC TGCAATGTCG ATGTGCAGCC TGAGTGCACC 
CTCATTCTCG TCGTGTACGC CTCCATGCCG TATCGTGAGG CGGCTGTGAC GGTCGCCCTT 
TATTCCGCCT CCGCCGTGGA AGTGGAGCCC GTGAAGGAGT GGGCGCAGGT CGCTGTGGCG 
GAGGGCAGCT GGGAGCTCGG CTACACGGCC GGCGGGGGAT CCGATCAGTT TGGCAGCTGG 
ATCAACAACC CGTTTGTGGC GCTAAATACG TATCGGCGGA CGCAAATCGT GGCGCTTCTG 
CTGCAGTATC CGCAGGGGCC GGACAAGCCT CTTGTGAAGC GTGCGGGAAA GAAAAAGGCA 
TTCTTGCCCC CCATTATCAT CAATCCAAAC AATAGAATGA TGATTGCGCT GGACCTGAAC 
GTGCAGAACA GCGAACTTAC ACCCATTGCC TCCACTCCCT ACACTTACAA CAGTGAGGTG 
ACACTTGTGG CGCACGTTCC TGCGGCAGAC TCGTTGCCAT TTCTTTTCAT TCCCCACACA 
AAACTACCCG AAGGGAATGG CGAGTTTAAA CTCTTTGTGT ACGCGGACTC CCCCATTGAA 
CTCTATACGC TTGAAAAGAA ACGCTTGCCT TACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053510121.170 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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