Tc00.1047053506957.80 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053506957.80
Entrez Gene ID	3546694
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_809950.1	XP_815043.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053506957.80 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053506957.80 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb09650	XM_809950.1 Latest version!	Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053506957.80 ( XM_809950.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_815043.1 Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.

Price & Availability↑

CloneID	OTb09650
Clone ID Related Accession (Same CDS sequence)	XM_809950.1
Accession Version	XM_809950.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849549). COMPLETENESS: incomplete on both ends.

ATGTTTGTAG AAAAGGTGGG AAGGGGAGTG AAAAATTGTC ATCTCATTGT ACTATGCAGC 
GATAGTCCTA TCTTTGATCT ACGCACGCTG CGTACCATCA TTTCTCATGC TGTTGCACTC 
GCATCGCCTT TGCCTCCTCC TCTTCATTCA GATGGTGCAT CCGTGACGTT GTTGCCATCG 
ACTGCGGCGG AGGACTATGA CGCGGAGAGC TGTCTCTCAA ATGCCGTGTT CTCGGCAATA 
CAGGGAAGCA CCCGTCGGCA TTCCTTTTCC AGTACGTGCA ATGGCACCAT TACGCCGTTG 
AACACTGCCA CGGGCGGAAA AGGCGGTAAA GCAACAAAGG GTGCCAAGAA ATCTGCAGCC 
GTGGCGGCAT CAACGAGTCG GGTGGCCACT CCCACAGAAG GAAAGAGCAT TGGGACAAAT 
GTTACCGGCC GGCCGCGTGC GGAGTGGTAC AGTGTTGTGG AGAGTCGTCA GTGGGGAGTT 
CCAGTTGTGT TTATCCACCA ACTGCCAGAA TTGGACCGAT CTGAGGGGGA AGGTGGTGCA 
CTGACACCGG CGGGCCAGGG AGCCGGCACC TCTGATTTCT GCACGCTGTT GCATAGGGCT 
TTGCAAATCA TTGCCGAGGA CATGAATGCC GCTGTGAGCC TTCATGAAGA CGCCCCAGCG 
GGGTTGTTAT CACTGGACGA CACCAATCTG AGTGAGATGC AGCACGAGGC ATTATCCGGA 
GAGCAGCCTC ATGTGCACGC GAAGGAAATT CCTGTTGTAA TGGCGTTGCA GGATCCCCGA 
GTATTACCAG GGTTGCTAAA TTGCAGAACA GCGTTGACCC ATCCTATTGA TACACGTCTC 
ATAGTGAATT TTGTTGATGG AAGACTCAAT GAATTATTGG GGACGTCTAA TCACGGTAGC 
TCAAGCACTC GTTCCCGCAC AAGTGCAGCT GGTAGACGCC AAAACCCCCG TGATATTTCT 
TCGTCCACGC TCCAAAAAAA TAGCAAAGAT GCGGATGGGA CCTGTGCTCC CAGTGATTCT 
AACAACACAC TCGATGCATG GATTGAACGG CAACAACGGG AGGGCATGAT AACAGTAACG 
ACATCTCCCA CAGGGGCCCC CAATGCATGT GGTGCGATTT GTCTCCGTGA GATATTTGAA 
GAGGGCGGGG AGGATAAACG TGTGTGGGTA CAACACTGCG TACAAAAGGT GTTGCCAGCG 
ATGCATCACG TTATACGCGA TATCACACGG TACGGTGAGT GGGTTTCTTC CAAGACGGTG 
TATTCTATTC CCATCACCTT GACCACACCA CGGCCACTTA TGCAGCCACA AACGAAAGAG 
GAAGGATTCC CCACCATGAG CGAGTCTGCC GTGGAATCAT CAGCGATGCT GCGATTAAGA 
AAATCACAAA CAACGGCTCC ACCATCTTCT TTTTCTCCCA CGACGTCGAC GAGGACGGGA 
GGTGTCGATG AGGACACTTT TCAGTTTCCT CCGCTAAGCG GAGATTTCTC GCTCTCTTTT 
TCTGCCACAA AACGGCTTTT AGATGGCGAA GAGGAAAAGT GGTCATATTA CGTTCGAGAG 
AGAAAAGTTC GTGGGCAATG GACCTGCATT TCTGCTGCCC GCGCCCTCGC TACGCAGGTG 
ACCGTTTCAT TGGCAAGACA GCAACGGCCT AGGGTGGTCT GTCCTGCGCG GGATGAGGAA 
AACTGCCCGT TACTGGATGT AATGAATGAA CGTAATGAGA CTTGTGCAGC GGACAAAATG 
AAAGAGAATG CTGTTGCCTC TCTGTTGTTA CAGCTCCGTC AATACGCCTC ACGCCGCACG 
GTGTTGTCGA CGTTGGATAC GGATATTTCT ACTGATATGG CTGCTGGAAA AGGTAGGGAA 
CCGAGTGTCG TTGCAGAAAC TCCAGGCGGT TCGCAACTAC TGGAGAATTC CACAGCTGGT 
GAGAAGAGCC AGAGTCAGTT TAACGCAATG GCAACGCTGC CCATGGATCA CACAATGGAC 
CGCCCAACAC ACCCACCGAG TCCGGAGGAG ATCCGATGGG TAACGCGACA ATTCATTAAA 
AAGGGAATTG GCGCCTTTCT CTTTCCGCCC GAAGAATCTT TGAGGGACGA TCCCCCATCC 
ATGCTTTCCC CTGATCAATT AAAATCTGCG ATAAAAATGC AGGAATTACT TGAGGAGACC 
TTGAATGGAC ATACATTGAA CGATGGTGAA GATGCGATGG ACAGTCAATT ATTACACCTT 
ATTGAAGAGG ACTTTAAACG CGCCGAGAAG GAAGTTTCAT GTGAATGGTC TCACGTGTGG 
GATAATATTG CTTTCCCTGT GGGTCTCATG AACTTCGATG AGAAGTTAAG ACGTTCTCTT 
GAGCGTAGTT TGACAACACG GGAGCGAACG CACATTTTAC AGCAGGTTTA TCTTTTGCAG 
TTAGCTATGC AGTCGTGTAT GACAGGTACA GCTGCCCCTA CCAATACTGT TGTTCTGCCA 
AATCACGGTA ATTGTAATTT ATTGACGCAC TCAAGCATAC CGAAGGATGA GGCGGTTGGT 
TTTAACTTGC GGTCTGTAGA GGAATGTATG ACGATGGCAG TGGCGATACG TCGTTTGTAT 
GAGTTTCAAT CTCGTTTCGG TTTCCATATT TGTCGTTTGA TACGTTGTTT CATACCAGAC 
ACGTTGGAAA CGTTGGGTGG GAATGTGACC GCCATGTATT CCAGACATGC GGAGAATGTG 
CATCCAACGA ATCGGGTGCG GGCGATGATG TTGGGAGGTG TGCCCATCCC ACGCAAGCGG 
CAACTGCGGC AAGTGTGGTG CTACTACGTT AGTGGTATTC CATCCGTTGA TGTGTTGAAT 
GAACTCCATG AATCCCAACG GGTGACGACA TCCTCCTATC CAGAGAGTTC GTCTGCTGCC 
CCTGTTGGTA TCACCACAAG GAACACGACG TCGACCTCCT TTGGGGGCGT CATTGGGGAT 
TTACTCCGTC AAAAGCTCCT TCAGAACCTG CAGGAATCAA CGCAGTTGTT ATCTTTGCCT 
GCAGAAAATA AAGTAAAAGT GAAAGGTGTA TGTTGCTCTG AAGGGGAATT GCACACAGCA 
CTGGAGGGAC GTCATATCTT GTATCCCTGT GAAAACAGTA TTGTTGAGGT TGTCACAACC 
GATCGAAGTC GCATCTGCCG CTACGTGAAG AGCAGTGAGT TGACATGTAC GCTACAGTAT 
TGCATCCCGC CATCACCACA ACTAAACACG AATACTGGCG GCGCCGATCC TGCCACGGCA 
TTCCCTTCTT CTTTGCCTTC GACGCTACGC AAACGGCCAC GTGATGCAAG TGCCCACTTC 
ACGACCACGT TTGATGACGG TGTGGTCTTC ACCTGCACCC CGCAGTGGAC AACACGTGCG 
GAACACGAGG GTCCCGGCGG TGCAGGTGAA GGGGAAAATA TCGCAAACGC CAGTTCGGCC 
TCTTTGAAAA GTGGAGTCTC ACGACGCAGT AAAGCAATGA GCAAAGCCGG TAGAAGAAGT 
GTGAACGTCT CCGTGGTGTT GGCAGGCGGT GGCCAACCGA GCGGAAGACG AGGACCCCTC 
TTGGGGAGGC AAAGACGACG TAGTCGTGAA GATGGTGAGG CGTTTGCCGC GGCGTCCGAG 
AGATCCGCTC GTACGGACCA TTTACCCCGC ATTTTTCCCG TTGCTCAGGA TGGATCTGTT 
TGCTTGTGGA CGGCGGGCCC TGAGAACATC CCGGGCAATG AAGCGGCGAA TGGGAGTGTA 
TCCGCAACGC CGAAGGCAGA GTTTGGCACG ATCCCCAATT TGGCGATTAC TGTGGCGGCA 
AATGGTGTGA CGGTGCACTG CGAGGAGCAG GAAGGGATTT TGTGGTTTAC GCAGCATGAG 
TCCAGCAATT CTCTGGTGCC GGAACTGCTA CGCTGTATGC ACGTGTATGA TGCGAATGCG 
ACATGGCATG AGACGATGGA AGTGGAGGTG CGTCGTGCCG TTTTGGAAGA ATTGGGGGCA 
GTTTGCCGCT ACTTCCACTC TGGTGTCACG CAGATGATTT ACCCAGATGG TGCGGTTGTC 
ACGCGGTACC CCATGCCAAC TAGCAATTTG GGTGGCGCTC AAGACTTCTG CGAGACACTC 
GTTACGGCAA CAGGAGCCTG CTACGTGCGT CAATTCCGTG GGGGATTTTT CGATCCGTTT 
CTAAATGCCA AAAGAAACAA CCTAAAGATG CATGTGGCGT ATGACCGTGG CAATCACTGC 
CGAGTTGTAT CTCGAGCAGA TGGCGTCATT ATAGCGTTTT ATTATTTTCA CCGCAACTCT 
CACGAGTCAA GAAAAAGTCA AGGAGGAGGC GGTAGAAATA TGAACCGTGA AAATGGCACG 
AAGGAACATC ATTCTGAAGA GGAAGTTGAT GCTTATGATA ATTATGATAG TGATGCGTGT 
GTGTGGGGTA ACTTCAAACA TCCTGCATTT GATACAAACG TCATTGCCCG CGTTACTTTA 
CATGCGGATA GCACGTGTAT CACGACCTTT TCAAGTCGAG CGACGCAAGA GACATGGGAA 
AACCCGCCAA GACCTTTGAA AACCTTGTTG GAGCATGTAA GTTTAGTTGG CAGGGCATGT 
AGTGGCACTG TACAGTATTG TGTTGAGGCT CCATCGTTGC CTCGTGTATT TGTGTGCGTC 
CCAATGTACA GTGATAATTG GAGCTTCTCG GAGGAACAGC AAGGTGTATC GATTCCGGGC 
ACATTTGACG CGGATTCATC GCTTGGGGCT CCCAATAACT CGTTTTTGAG TAAATGCAGC 
AGTGCGAGGG CAGCCGATGT TCACCCCTCA TCTCTTCTGG CGAGTCCCGT GCCTTTACCT 
GCCTCTGGTG TAAAGGAACG TTTTTCTCCC GTCTCCCCAC GATGTAAGCC GTATGACAGT 
TTTTACATTG TGTTTGGTGA TGGCTCCGTG CTGCGGCGAC GCGTCTTGTA CCGCGGGGTG 
CGTGGGACCG TTACTTCATT TTTAGAAACG CTCTTTGCGC GTTCCACCTC AACATCCATC 
CGTGTGATTC ATGAGTCTGG ACTGGCCATT GTGGAACCGT TTGAGGCGCT GCGCCACAAC 
ATGGAGGCAG TGGCATCCCT TGCGATTGGT GAGGGTTTGG CAATGTTCGA CATTGCGTTG 
GGCGGGTTGC GGCTGGTCGA CCATCAACAT CACCTGACGG AGGTACATGG TCTGTACTCA 
CCGGGTCCGG TGAGTGCGGG GATTAAATCC ACCACGCTTG AATTTCTCCT ACGTCAGCTC 
GTGTTACCTA CATACACACC CCATACTGTC ACCAAGACAC GACAGGAGGA GATGCGGCAA 
GATGAAAAGG TGGCAGATGC AGAGAGCCGG GCGAATCGTG CCAAGGGTAT ATGTCCCCCG 
TTGGCAACTC GTCTGCGAGA ACTTGCCGAG GGTTTTGTCA TTGAGCACAA TCTTCTTCGC 
ACCGATATTC TTAACACGGC CACGCGCCAA TGGGAAGGCG GCAATTTGGA GACAATCAAT 
AAAAAATCTA TGCAGGACGT CACGTCGGGA GCATCGTGGG GCAAACTTAC GACGCAAGTG 
GGAAAGTGCG GCTCCGCGGC GCTAAAGGCC CTTGAGTCGC CTGCCGTGCC TGCGACAATT 
ACTCGAACCG TTGGTGTGTT GCCTATTTTA TTTTCCGAGT TCGAGAACGA TGTTGTGGTG 
CAGTACATTC ATCAGCATGA TGTGGAGAAC TACATGCATG CGAAGGAGTC AGAGCCCATC 
CCTGTCTTTC TGTCGCACAG CACCGCAGCG GGGGAACCCG GAGTGCAGCA GTTGACGTTC 
TTTCGTGCAG AGGCGAATTC AGGAACCGCA GATGCGCCAA TCGCAAGAGA ACCCACATCG 
CGATTTTTTC TAATGAGCCG TTCATTTTCG GGTCAGTTGT GCAGCTCCAG CGGTTTACCG 
ATCGGGCAAG GCGGTGCGGT AGGGACGGGA TCTCTTTTGA GTGAGGCCGT GCAGCGGCAG 
GAGAAACTTT CCTTCTTTTT AGGGAGACGA CTGGTGTTTC CAACGACGCA GTGGCTTCCA 
CCAGCGATGC AGCCACCCCG CCGTTTTTTG AAGCCACTCT TCGATCCCGA GGTGAATGAC 
AAGCGTGCCG GCATTACAGT TCTGGCGGGC GAAAGCGTTG GCAACAATGG TGGGATTGAC 
GGTTCACCCT CCTCGTACGA GAAGTTGCGT ATTCTTTTTA AGTTCTCCGA CGTCGCGCCG 
AAGACTCAAC GGGCTGTGTT GGCAAGTGAA ATTCTGCGGC ATGAGCAGCT GTCCTCCATG 
GTGACGCACC AAAAGGCTAT AAATGCATTG GCACCGAAGA TTCCATACGA GGCCAACAAT 
GAACAGCGGC GTCTTCGTGA AAAATACCTC GCAGCAGCCA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_815043.1
CDS	1..6345
Translation	MFVEKVGRGVKNCHLIVLCSDSPIFDLRTLRTIISHAVALASPLPPPLHSDGASVTLLPSTAAEDYDAESCLSNAVFSAIQGSTRRHSFSSTCNGTITPLNTATGGKGGKATKGAKKSAAVAASTSRVATPTEGKSIGTNVTGRPRAEWYSVVESRQWGVPVVFIHQLPELDRSEGEGGALTPAGQGAGTSDFCTLLHRALQIIAEDMNAAVSLHEDAPAGLLSLDDTNLSEMQHEALSGEQPHVHAKEIPVVMALQDPRVLPGLLNCRTALTHPIDTRLIVNFVDGRLNELLGTSNHGSSSTRSRTSAAGRRQNPRDISSSTLQKNSKDADGTCAPSDSNNTLDAWIERQQREGMITVTTSPTGAPNACGAICLREIFEEGGEDKRVWVQHCVQKVLPAMHHVIRDITRYGEWVSSKTVYSIPITLTTPRPLMQPQTKEEGFPTMSESAVESSAMLRLRKSQTTAPPSSFSPTTSTRTGGVDEDTFQFPPLSGDFSLSFSATKRLLDGEEEKWSYYVRERKVRGQWTCISAARALATQVTVSLARQQRPRVVCPARDEENCPLLDVMNERNETCAADKMKENAVASLLLQLRQYASRRTVLSTLDTDISTDMAAGKGREPSVVAETPGGSQLLENSTAGEKSQSQFNAMATLPMDHTMDRPTHPPSPEEIRWVTRQFIKKGIGAFLFPPEESLRDDPPSMLSPDQLKSAIKMQELLEETLNGHTLNDGEDAMDSQLLHLIEEDFKRAEKEVSCEWSHVWDNIAFPVGLMNFDEKLRRSLERSLTTRERTHILQQVYLLQLAMQSCMTGTAAPTNTVVLPNHGNCNLLTHSSIPKDEAVGFNLRSVEECMTMAVAIRRLYEFQSRFGFHICRLIRCFIPDTLETLGGNVTAMYSRHAENVHPTNRVRAMMLGGVPIPRKRQLRQVWCYYVSGIPSVDVLNELHESQRVTTSSYPESSSAAPVGITTRNTTSTSFGGVIGDLLRQKLLQNLQESTQLLSLPAENKVKVKGVCCSEGELHTALEGRHILYPCENSIVEVVTTDRSRICRYVKSSELTCTLQYCIPPSPQLNTNTGGADPATAFPSSLPSTLRKRPRDASAHFTTTFDDGVVFTCTPQWTTRAEHEGPGGAGEGENIANASSASLKSGVSRRSKAMSKAGRRSVNVSVVLAGGGQPSGRRGPLLGRQRRRSREDGEAFAAASERSARTDHLPRIFPVAQDGSVCLWTAGPENIPGNEAANGSVSATPKAEFGTIPNLAITVAANGVTVHCEEQEGILWFTQHESSNSLVPELLRCMHVYDANATWHETMEVEVRRAVLEELGAVCRYFHSGVTQMIYPDGAVVTRYPMPTSNLGGAQDFCETLVTATGACYVRQFRGGFFDPFLNAKRNNLKMHVAYDRGNHCRVVSRADGVIIAFYYFHRNSHESRKSQGGGGRNMNRENGTKEHHSEEEVDAYDNYDSDACVWGNFKHPAFDTNVIARVTLHADSTCITTFSSRATQETWENPPRPLKTLLEHVSLVGRACSGTVQYCVEAPSLPRVFVCVPMYSDNWSFSEEQQGVSIPGTFDADSSLGAPNNSFLSKCSSARAADVHPSSLLASPVPLPASGVKERFSPVSPRCKPYDSFYIVFGDGSVLRRRVLYRGVRGTVTSFLETLFARSTSTSIRVIHESGLAIVEPFEALRHNMEAVASLAIGEGLAMFDIALGGLRLVDHQHHLTEVHGLYSPGPVSAGIKSTTLEFLLRQLVLPTYTPHTVTKTRQEEMRQDEKVADAESRANRAKGICPPLATRLRELAEGFVIEHNLLRTDILNTATRQWEGGNLETINKKSMQDVTSGASWGKLTTQVGKCGSAALKALESPAVPATITRTVGVLPILFSEFENDVVVQYIHQHDVENYMHAKESEPIPVFLSHSTAAGEPGVQQLTFFRAEANSGTADAPIAREPTSRFFLMSRSFSGQLCSSSGLPIGQGGAVGTGSLLSEAVQRQEKLSFFLGRRLVFPTTQWLPPAMQPPRRFLKPLFDPEVNDKRAGITVLAGESVGNNGGIDGSPSSYEKLRILFKFSDVAPKTQRAVLASEILRHEQLSSMVTHQKAINALAPKIPYEANNEQRRLREKYLAAAT

Target ORF information:

RefSeq Version	XM_809950.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_809950.1

ORF Insert Sequence:

ATGTTTGTAG AAAAGGTGGG AAGGGGAGTG AAAAATTGTC ATCTCATTGT ACTATGCAGC 
GATAGTCCTA TCTTTGATCT ACGCACGCTG CGTACCATCA TTTCTCATGC TGTTGCACTC 
GCATCGCCTT TGCCTCCTCC TCTTCATTCA GATGGTGCAT CCGTGACGTT GTTGCCATCG 
ACTGCGGCGG AGGACTATGA CGCGGAGAGC TGTCTCTCAA ATGCCGTGTT CTCGGCAATA 
CAGGGAAGCA CCCGTCGGCA TTCCTTTTCC AGTACGTGCA ATGGCACCAT TACGCCGTTG 
AACACTGCCA CGGGCGGAAA AGGCGGTAAA GCAACAAAGG GTGCCAAGAA ATCTGCAGCC 
GTGGCGGCAT CAACGAGTCG GGTGGCCACT CCCACAGAAG GAAAGAGCAT TGGGACAAAT 
GTTACCGGCC GGCCGCGTGC GGAGTGGTAC AGTGTTGTGG AGAGTCGTCA GTGGGGAGTT 
CCAGTTGTGT TTATCCACCA ACTGCCAGAA TTGGACCGAT CTGAGGGGGA AGGTGGTGCA 
CTGACACCGG CGGGCCAGGG AGCCGGCACC TCTGATTTCT GCACGCTGTT GCATAGGGCT 
TTGCAAATCA TTGCCGAGGA CATGAATGCC GCTGTGAGCC TTCATGAAGA CGCCCCAGCG 
GGGTTGTTAT CACTGGACGA CACCAATCTG AGTGAGATGC AGCACGAGGC ATTATCCGGA 
GAGCAGCCTC ATGTGCACGC GAAGGAAATT CCTGTTGTAA TGGCGTTGCA GGATCCCCGA 
GTATTACCAG GGTTGCTAAA TTGCAGAACA GCGTTGACCC ATCCTATTGA TACACGTCTC 
ATAGTGAATT TTGTTGATGG AAGACTCAAT GAATTATTGG GGACGTCTAA TCACGGTAGC 
TCAAGCACTC GTTCCCGCAC AAGTGCAGCT GGTAGACGCC AAAACCCCCG TGATATTTCT 
TCGTCCACGC TCCAAAAAAA TAGCAAAGAT GCGGATGGGA CCTGTGCTCC CAGTGATTCT 
AACAACACAC TCGATGCATG GATTGAACGG CAACAACGGG AGGGCATGAT AACAGTAACG 
ACATCTCCCA CAGGGGCCCC CAATGCATGT GGTGCGATTT GTCTCCGTGA GATATTTGAA 
GAGGGCGGGG AGGATAAACG TGTGTGGGTA CAACACTGCG TACAAAAGGT GTTGCCAGCG 
ATGCATCACG TTATACGCGA TATCACACGG TACGGTGAGT GGGTTTCTTC CAAGACGGTG 
TATTCTATTC CCATCACCTT GACCACACCA CGGCCACTTA TGCAGCCACA AACGAAAGAG 
GAAGGATTCC CCACCATGAG CGAGTCTGCC GTGGAATCAT CAGCGATGCT GCGATTAAGA 
AAATCACAAA CAACGGCTCC ACCATCTTCT TTTTCTCCCA CGACGTCGAC GAGGACGGGA 
GGTGTCGATG AGGACACTTT TCAGTTTCCT CCGCTAAGCG GAGATTTCTC GCTCTCTTTT 
TCTGCCACAA AACGGCTTTT AGATGGCGAA GAGGAAAAGT GGTCATATTA CGTTCGAGAG 
AGAAAAGTTC GTGGGCAATG GACCTGCATT TCTGCTGCCC GCGCCCTCGC TACGCAGGTG 
ACCGTTTCAT TGGCAAGACA GCAACGGCCT AGGGTGGTCT GTCCTGCGCG GGATGAGGAA 
AACTGCCCGT TACTGGATGT AATGAATGAA CGTAATGAGA CTTGTGCAGC GGACAAAATG 
AAAGAGAATG CTGTTGCCTC TCTGTTGTTA CAGCTCCGTC AATACGCCTC ACGCCGCACG 
GTGTTGTCGA CGTTGGATAC GGATATTTCT ACTGATATGG CTGCTGGAAA AGGTAGGGAA 
CCGAGTGTCG TTGCAGAAAC TCCAGGCGGT TCGCAACTAC TGGAGAATTC CACAGCTGGT 
GAGAAGAGCC AGAGTCAGTT TAACGCAATG GCAACGCTGC CCATGGATCA CACAATGGAC 
CGCCCAACAC ACCCACCGAG TCCGGAGGAG ATCCGATGGG TAACGCGACA ATTCATTAAA 
AAGGGAATTG GCGCCTTTCT CTTTCCGCCC GAAGAATCTT TGAGGGACGA TCCCCCATCC 
ATGCTTTCCC CTGATCAATT AAAATCTGCG ATAAAAATGC AGGAATTACT TGAGGAGACC 
TTGAATGGAC ATACATTGAA CGATGGTGAA GATGCGATGG ACAGTCAATT ATTACACCTT 
ATTGAAGAGG ACTTTAAACG CGCCGAGAAG GAAGTTTCAT GTGAATGGTC TCACGTGTGG 
GATAATATTG CTTTCCCTGT GGGTCTCATG AACTTCGATG AGAAGTTAAG ACGTTCTCTT 
GAGCGTAGTT TGACAACACG GGAGCGAACG CACATTTTAC AGCAGGTTTA TCTTTTGCAG 
TTAGCTATGC AGTCGTGTAT GACAGGTACA GCTGCCCCTA CCAATACTGT TGTTCTGCCA 
AATCACGGTA ATTGTAATTT ATTGACGCAC TCAAGCATAC CGAAGGATGA GGCGGTTGGT 
TTTAACTTGC GGTCTGTAGA GGAATGTATG ACGATGGCAG TGGCGATACG TCGTTTGTAT 
GAGTTTCAAT CTCGTTTCGG TTTCCATATT TGTCGTTTGA TACGTTGTTT CATACCAGAC 
ACGTTGGAAA CGTTGGGTGG GAATGTGACC GCCATGTATT CCAGACATGC GGAGAATGTG 
CATCCAACGA ATCGGGTGCG GGCGATGATG TTGGGAGGTG TGCCCATCCC ACGCAAGCGG 
CAACTGCGGC AAGTGTGGTG CTACTACGTT AGTGGTATTC CATCCGTTGA TGTGTTGAAT 
GAACTCCATG AATCCCAACG GGTGACGACA TCCTCCTATC CAGAGAGTTC GTCTGCTGCC 
CCTGTTGGTA TCACCACAAG GAACACGACG TCGACCTCCT TTGGGGGCGT CATTGGGGAT 
TTACTCCGTC AAAAGCTCCT TCAGAACCTG CAGGAATCAA CGCAGTTGTT ATCTTTGCCT 
GCAGAAAATA AAGTAAAAGT GAAAGGTGTA TGTTGCTCTG AAGGGGAATT GCACACAGCA 
CTGGAGGGAC GTCATATCTT GTATCCCTGT GAAAACAGTA TTGTTGAGGT TGTCACAACC 
GATCGAAGTC GCATCTGCCG CTACGTGAAG AGCAGTGAGT TGACATGTAC GCTACAGTAT 
TGCATCCCGC CATCACCACA ACTAAACACG AATACTGGCG GCGCCGATCC TGCCACGGCA 
TTCCCTTCTT CTTTGCCTTC GACGCTACGC AAACGGCCAC GTGATGCAAG TGCCCACTTC 
ACGACCACGT TTGATGACGG TGTGGTCTTC ACCTGCACCC CGCAGTGGAC AACACGTGCG 
GAACACGAGG GTCCCGGCGG TGCAGGTGAA GGGGAAAATA TCGCAAACGC CAGTTCGGCC 
TCTTTGAAAA GTGGAGTCTC ACGACGCAGT AAAGCAATGA GCAAAGCCGG TAGAAGAAGT 
GTGAACGTCT CCGTGGTGTT GGCAGGCGGT GGCCAACCGA GCGGAAGACG AGGACCCCTC 
TTGGGGAGGC AAAGACGACG TAGTCGTGAA GATGGTGAGG CGTTTGCCGC GGCGTCCGAG 
AGATCCGCTC GTACGGACCA TTTACCCCGC ATTTTTCCCG TTGCTCAGGA TGGATCTGTT 
TGCTTGTGGA CGGCGGGCCC TGAGAACATC CCGGGCAATG AAGCGGCGAA TGGGAGTGTA 
TCCGCAACGC CGAAGGCAGA GTTTGGCACG ATCCCCAATT TGGCGATTAC TGTGGCGGCA 
AATGGTGTGA CGGTGCACTG CGAGGAGCAG GAAGGGATTT TGTGGTTTAC GCAGCATGAG 
TCCAGCAATT CTCTGGTGCC GGAACTGCTA CGCTGTATGC ACGTGTATGA TGCGAATGCG 
ACATGGCATG AGACGATGGA AGTGGAGGTG CGTCGTGCCG TTTTGGAAGA ATTGGGGGCA 
GTTTGCCGCT ACTTCCACTC TGGTGTCACG CAGATGATTT ACCCAGATGG TGCGGTTGTC 
ACGCGGTACC CCATGCCAAC TAGCAATTTG GGTGGCGCTC AAGACTTCTG CGAGACACTC 
GTTACGGCAA CAGGAGCCTG CTACGTGCGT CAATTCCGTG GGGGATTTTT CGATCCGTTT 
CTAAATGCCA AAAGAAACAA CCTAAAGATG CATGTGGCGT ATGACCGTGG CAATCACTGC 
CGAGTTGTAT CTCGAGCAGA TGGCGTCATT ATAGCGTTTT ATTATTTTCA CCGCAACTCT 
CACGAGTCAA GAAAAAGTCA AGGAGGAGGC GGTAGAAATA TGAACCGTGA AAATGGCACG 
AAGGAACATC ATTCTGAAGA GGAAGTTGAT GCTTATGATA ATTATGATAG TGATGCGTGT 
GTGTGGGGTA ACTTCAAACA TCCTGCATTT GATACAAACG TCATTGCCCG CGTTACTTTA 
CATGCGGATA GCACGTGTAT CACGACCTTT TCAAGTCGAG CGACGCAAGA GACATGGGAA 
AACCCGCCAA GACCTTTGAA AACCTTGTTG GAGCATGTAA GTTTAGTTGG CAGGGCATGT 
AGTGGCACTG TACAGTATTG TGTTGAGGCT CCATCGTTGC CTCGTGTATT TGTGTGCGTC 
CCAATGTACA GTGATAATTG GAGCTTCTCG GAGGAACAGC AAGGTGTATC GATTCCGGGC 
ACATTTGACG CGGATTCATC GCTTGGGGCT CCCAATAACT CGTTTTTGAG TAAATGCAGC 
AGTGCGAGGG CAGCCGATGT TCACCCCTCA TCTCTTCTGG CGAGTCCCGT GCCTTTACCT 
GCCTCTGGTG TAAAGGAACG TTTTTCTCCC GTCTCCCCAC GATGTAAGCC GTATGACAGT 
TTTTACATTG TGTTTGGTGA TGGCTCCGTG CTGCGGCGAC GCGTCTTGTA CCGCGGGGTG 
CGTGGGACCG TTACTTCATT TTTAGAAACG CTCTTTGCGC GTTCCACCTC AACATCCATC 
CGTGTGATTC ATGAGTCTGG ACTGGCCATT GTGGAACCGT TTGAGGCGCT GCGCCACAAC 
ATGGAGGCAG TGGCATCCCT TGCGATTGGT GAGGGTTTGG CAATGTTCGA CATTGCGTTG 
GGCGGGTTGC GGCTGGTCGA CCATCAACAT CACCTGACGG AGGTACATGG TCTGTACTCA 
CCGGGTCCGG TGAGTGCGGG GATTAAATCC ACCACGCTTG AATTTCTCCT ACGTCAGCTC 
GTGTTACCTA CATACACACC CCATACTGTC ACCAAGACAC GACAGGAGGA GATGCGGCAA 
GATGAAAAGG TGGCAGATGC AGAGAGCCGG GCGAATCGTG CCAAGGGTAT ATGTCCCCCG 
TTGGCAACTC GTCTGCGAGA ACTTGCCGAG GGTTTTGTCA TTGAGCACAA TCTTCTTCGC 
ACCGATATTC TTAACACGGC CACGCGCCAA TGGGAAGGCG GCAATTTGGA GACAATCAAT 
AAAAAATCTA TGCAGGACGT CACGTCGGGA GCATCGTGGG GCAAACTTAC GACGCAAGTG 
GGAAAGTGCG GCTCCGCGGC GCTAAAGGCC CTTGAGTCGC CTGCCGTGCC TGCGACAATT 
ACTCGAACCG TTGGTGTGTT GCCTATTTTA TTTTCCGAGT TCGAGAACGA TGTTGTGGTG 
CAGTACATTC ATCAGCATGA TGTGGAGAAC TACATGCATG CGAAGGAGTC AGAGCCCATC 
CCTGTCTTTC TGTCGCACAG CACCGCAGCG GGGGAACCCG GAGTGCAGCA GTTGACGTTC 
TTTCGTGCAG AGGCGAATTC AGGAACCGCA GATGCGCCAA TCGCAAGAGA ACCCACATCG 
CGATTTTTTC TAATGAGCCG TTCATTTTCG GGTCAGTTGT GCAGCTCCAG CGGTTTACCG 
ATCGGGCAAG GCGGTGCGGT AGGGACGGGA TCTCTTTTGA GTGAGGCCGT GCAGCGGCAG 
GAGAAACTTT CCTTCTTTTT AGGGAGACGA CTGGTGTTTC CAACGACGCA GTGGCTTCCA 
CCAGCGATGC AGCCACCCCG CCGTTTTTTG AAGCCACTCT TCGATCCCGA GGTGAATGAC 
AAGCGTGCCG GCATTACAGT TCTGGCGGGC GAAAGCGTTG GCAACAATGG TGGGATTGAC 
GGTTCACCCT CCTCGTACGA GAAGTTGCGT ATTCTTTTTA AGTTCTCCGA CGTCGCGCCG 
AAGACTCAAC GGGCTGTGTT GGCAAGTGAA ATTCTGCGGC ATGAGCAGCT GTCCTCCATG 
GTGACGCACC AAAAGGCTAT AAATGCATTG GCACCGAAGA TTCCATACGA GGCCAACAAT 
GAACAGCGGC GTCTTCGTGA AAAATACCTC GCAGCAGCCA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053506957.80 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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