Tc00.1047053510003.30 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053510003.30
Entrez Gene ID	3539564
Full Name	phosphatidylinositol-kinase domain protein
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_803923.1	XP_809016.1	phosphatidylinositol-kinase domain protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053510003.30 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053510003.30 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb04429	XM_803923.1 Latest version!	Trypanosoma cruzi strain CL Brener phosphatidylinositol-kinase domain protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053510003.30 ( XM_803923.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_809016.1 Trypanosoma cruzi strain CL Brener phosphatidylinositol-kinase domain protein partial mRNA.

Price & Availability↑

CloneID	OTb04429
Clone ID Related Accession (Same CDS sequence)	XM_803923.1
Accession Version	XM_803923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	phosphatidylinositol-kinase domain protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849507). COMPLETENESS: incomplete on both ends.

ATGGACGAAA TCCCATTTCC CACATGGGTG CGGCGTTCGC CGGTCTTTGC AATGACGCGG 
GTGCTGGGCC AACACCTGCT GCAACAGTTG CAACCCACGC GTGTAGAACC AACAGGACCA 
GTCGTGGTGG AAACTACAAC CACAAACATC ACAACAAAAA GGGCAGGGAC TGCGTCGTCG 
TCGCCCATAC GGCGAAAATG CGGGGAGGTT ACTCCTCTCA ACACGAGGTC TCTTAGCAAC 
TCCGAATACT TTTCTCCAGT AAGATTATTT GGCTTAGACG AGCAAAATAC AGGGGTTTTT 
GTAGACAATG TTGCGCGGCT TAACGAAACA AACACAACTT CTAACGAGTT TGGGGTGCAG 
CAGCGCTCTT GCAACTCCAC GGGGATGCGG TTGGGCGACG TTCTGAGCCA CCCGGGCGTT 
GCGCTACTGA TTAGTTTGTC GCAGATCAAC GAGCTCGTGT CACAAAAGGT CGGGGTGACT 
GTCAAGAACG CCCCAGCTTT GGTTTCCCTC ATGGCTCTTG CAGTTGAGTG GCGTTTTTTG 
CCGTATGAAT ACACCAAAGG GTTTTATTCT GAGTGCGCCT CCTTTTACAC ATTGTTGATG 
CGTCAGCTTG GAACCACAAA GTTTTTCCCA GGTGTTGACG TGCAGGGATT TGTGGCGTCG 
CTTGTATTTT GTGGGATGCT GCTGCTGCGG GGCAACGGAG AGGGGCAGAA GGACGGTGAA 
AAGGATGGGG AGGAGACGAA TGATCATGCC AGCCGCCGTG TCCACGATGC GTACATCAGC 
CAGAGCAGGG GGAGAAATAT TGGTCTGGCA GGGTTTGATG GCTGCATCAT CGACTCCATC 
ATCATTTCCT GCATTCCATC CGATATTGAC GAGTGCGTTG AGAACCCCAT TGCTCTTGGT 
GCTCTGAATG GACTCGCCCA CACAACGAAA AGTGAACCCA TTTTTCTGAG CTCGCAGCTG 
CTGAATCATT TACAAAATTT GACAGACGCC TTTACAAATT CATCCAGGCG AAGGGAACCG 
TTGCACAAGC GGCTTCTTTG TTACCTCATG GAGGCGATTT GCTGCGCTCG AGGTGTAAAT 
TACACGCGCA TGCGCAACTG GCTTCGGGCC GTGCTTCAAC CGGATTACGT ATTACGAGGC 
CTCATGCAGA AAGAAGACCT GCATGCGCGC GTGGTCGCCG CGATGCTGAC GCTCCTGACG 
GTGTGGATTC AAGACTCCGT AACAACATGT ACGGTAAAAT ACAAAGGTGC GGGTGATGCT 
CAAGACGAAA TCTCTTCATT TTTCTCCCGC GAAGAATGCA CAGAGGCTGA TGCACTTCTA 
GAGAGTGTTT GGTCTGCCCT CCAAGAGGCT GCGAGCCGAT GGGAGGATCT TCGAAAGACA 
AACGTCAAAC TCTACGGTGG TAACGTGCGC ATTGTCGTGA AAAATTTATT ACAGGCAGAA 
TTGGTTCGTG CGCAGTGCTA CAGAACCATT AGTGGCATGT ATCAAGAGCA ACAAGTGCAG 
CAGGGGGATA ATACCTGGAC AGGAAGTTAT TTGCCTTCAG CCGAGGAGGA AGATCTCCGC 
CTCCGTGTGA TTGGAAACAT CGTTAAATTT CTTATCCTTC CGACGAGCAT GTTTAGTTTC 
ATAACGGCGA AGGCGTCAGC GACCCGACTT CGACAGGACA TCGCCGAGCA ATGCGTAAAG 
GTCATTGCGT TGACCACTTC TGCAGAGGGC AAAGCTATCT CTCTTTGGAA AAACGTCTCC 
ACACCCTCTA CTGTGACGAA GGCACAGTAT TCACACAACT ACCACATCAA TATTGATGGC 
ATCGTCCGCG AGTTGATCCA CCGCGTACTG GAGGTTGGAA CTTTGGAGAC TTTTCAAGCC 
ACGCCTCTGG ATGAAGAAGG GGATGAAGGT CGCATGGCGG CATTATGTCT TCAGGGACTT 
TGTGCCACAA GTTCCCATGT GCGGTCCTTT GCGGTGACGG AGGTGTTGGA TTCCGTTGTG 
CGTCTCTCAA CGGTGCTCCA GTCTGTTGAT GCCGAGAGTA GCAGCACGGA TAAAAACGCA 
TCGATTTCGT CAAGCCGAGC TGCTCCGCGT TTAGCAGTGT GCTTTGAGAT GATGAATCGT 
CTGCTTGTGT CGCGGCACCT TTCCATTTTG AAGCCGCTCA CTTGTGATGC CTTGTTACGC 
TTTGGAAAAG TTTTTTGGGA CACGTTCGAG GACACCCTGC GAGTTCATGT GCGTTCTGGC 
GATCTCGCCA ACCCCACGAT AAAGTTTCCC AGTGCCACGC TGCTGATGGT TTTGGAATCC 
TTCTGGGGAC TTGTGCAGCG TTGTCACGAA CGCATTCGTG TCATGACACG GGAGGGTCTT 
CGTCTTGTCG CTTCTGGGGA TGGGGAGGCA AAGGCACGCC TGGTTGAAGC GTTTAACGCG 
GAGAGCCGCG TCCTCCGTGT ACTTTTGCGG ACTTTACTGC GTTGCATGAT TAATGTTGCG 
TGGCCCGGGT CTGGCATTCT CTACGGTAAA GCCTCCGAGA GGGGCACTCA TCATCCGGCC 
GTGTTTGATG TGCAACGTAT TGCCGTTGAG GTGCTGTCAC TATTTCTCCG TCCCCTCTGC 
TTGCTATGCC AGCTTTCCAC ACTGTTTCCC GTGACGGATA TCCGACACCT TGTGTATCAC 
CATGAAAATG CACAAAAGCT CACGCAGTTG GATGAAGGGG AGTCTTTCTT GTACCACTAC 
TGTTTTCAAC CATCTAATAT CAACTCTTCT TTCTATGCCG AAAATGGGAT GGCACAGTTC 
CGTTCTTGTT GGCTCATGTT ATCATACTAC CGTCTTACAA GAGAAGCACT CTTGCGGACC 
GAAACGGATG CGACGAGTAC TGCCTCAGAC AAACTGCGCT GCCAGGAAAA GGGTTTAAAT 
TCAATGGATT GGACCAAATG TATACGATTG CTTGCCGCGT CCATGCCGCC TCTTATGCAT 
ATGCGATCCT CCGATTATCA GCAAACCATG GCAGACATAG ATGTTTTGAA ACATCGGTTA 
TTCAGCAGTG GTGATTTAGG TGACGGTAAG TTTTATCAGA AGGAGTTGGT GCGTTTTCTT 
CAACGCTACT GTCCAGAGGC CAAACATGTA ATTAAGCGTC TTTCTTTTTC GGAGGCCTTT 
TTGATAAATG CTCTCGCGAC ATTGGAGATG CTACGTGCAT CATGTGGCTC TATCTCCACC 
ATAACGCATT ATCAACATTA TGAGCTACGG GAGTTTGATG CCAGTAAGGA TATTAGAAAA 
ATTGTGAAGC ATGTAACAAA GGCTGCCACG GCACGATATA TCTCTGCTCT CGGAGAAACG 
CTCCCGGATT TTGCGGCGAG TATCATTGAT AAGAATGCGC GGCAGCTTGT TTTGCTCTAC 
GGATTTGCTA TTGACTCTGT ACGGAGTTCT GCAAAGGAGA TTCTTCGCAA TATTATTTCT 
AGCTTTCCCG CCTTTGCGAC AAGCAGCACG GCCCTTCCTC TCTTGTGGGC GGTGATCAAT 
GTGCTGGAGG TAGGGAACGC CACGGAGGTG GAACGTTTCT GTGAGCATCT GCAGTTTCCA 
GAGGCGCCAG CTGTTGCCAC GGATCCTGAT TCTCTTGAAC GGCGAAATCG GCTACAGGAT 
GCTGTGGCAT TTGCGGAGTA TTGGGCCCAG GCGGCGCGTA AATTCTCGCC TTTGGCACTT 
GTGGAGCACG CCACCTTATT TATTGTTGAG GAGGAGGCCA CATCTGGGGT GATCGGCTCT 
GCTGCCGGCT CGAGACTTGC CCTTCGTGCA CACAACATGG ACACTGCACC AGGCCATCCT 
AACCAGAATT ACGCCCAAAT TCTCATTAAG AGGAGCAATG CCCAAGGGCA AGTGATGGCA 
GCCTTTCGTT TGACTCCACA TTGTGGGGTG GGCGTTCTTC TTCTTCGCCA GCTTCAGGCG 
ATGGTGAGGG ACAGGCTTGG AACGGTAACC GTGACCGCAA AGTCCCACGA GCGGCTTTTT 
GCGTCCTTGG TGAGCACCGT TCACGCGGTC ACCAAGGTGG GGCCATGTGC CGAAGACGAA 
GACTGCAACG ACACCGGGAC TGAGGCTGGT GAGGGGATAT CGCATGAAGA ACCATCCACG 
CGCGGCGACC AGGCTGCATT ACGAAGGCTT TCCGAGGCCG ATACGCTTCT TTGCACGGTT 
GCCGCATTCT TGGTCTCCGC ACGGAGTTCT CCTTCGACGC AATTGGAACT TCTGTGGCAT 
CTTGTGCGGG GACCCATTTT TTTGTTTTCA AGTCACTACA TATCGGCTGC CATTCAGGGT 
TGGCAGTGGC TTTTATTTGA GAAGCCCGAG CGCTATGCGG TGCCGTTGTT GGTACATTTG 
GTGGAGGGGT TTATCTGGAC GATTGTGCAG AGACTTGGTC TTTTTGACGG CACCAGACCT 
TTTCAGGCGG ATGAAGTGGA GTCGGGGGAG AAAACGACTT CGATTCAGTG CGAGGCTTTA 
TACCCCAAGG AATATAATGT GAACTCCCCG CATAAACTTC TTATCGAGTT TCTCTCGGAT 
TGCTACGTGG ACATTCGTGG GCCTGTGTCT TTTCATCCGT CTGTGCTTCT TCTCCTGCAC 
CACCTTGCCA TGCGAGTGGT TGAGGCACCA TCCCGATTCT CCACGAAAGA TAGTTCCTTT 
TCGGAAATGA TGCGTTGCGC CCTATTAATG AGTTCCATAT GCCATAATTT GCAGATTGTC 
AATGAACGTC GCTTAAGTAT TGGATCACCC GCTTTTGTGG CATCCGCTTC TATTGGAAAA 
GTTCGTCAAG GCTGGTACAA GTGTCTGTTG CAATGGTTTC ACAAGACTCC GCCTTCGTGG 
TACTTTACGC GAGATCCCTC TGCCGCAGAG GAGGAGTTGG TGGTGCTTCA GAAGCTCATA 
AAAGTACTTG ATGCGGATCG AGATGCGTTG CTGCGTTCTA CGCATGGCTT TCTTGATTTT 
GACCCGTCTA AAATTCATGC TTGTACGGCT GCGAAGTTTA CTCTTATGGG AAGTAGTGTG 
CGTCATACAC TCTGTGACAT GCTCTCGGAC GATTCAGTGA AGGCGGAGGG GGTGATTCGG 
GGGGTGATCA CCAGTGAACG AAACCGCCTT TTGGATCTTA TTGCGCTAAT GAGACTTTTG 
GTGGAGCACG AACGCACACG ACTTGCGGTG TGGCTTCGGC CACGTGTAGG GGCTGGCGCC 
CGGTTGGGGG AGGGGAGTTT GAATGGCCTT TCGGAGGCCG TGTGGAGGAG GCATTGTGAG 
GCGGCTGCGA GGAATAACCC TGCGGTGTTA CTGGCGTTGG TGGCAAGATT TCCCAGTCCA 
CATTTATTGG GGTCCGCCAC GCGGCATGTA ACCACCAACC CCGAAGCCTA TTGCCACCTA 
CCCGAGGCAG TTGATCTTTA CCTTAACGAG GAGGTGCTTA GGAGTGGTTA TCCCGCCCTC 
GGTTTATTTA CAAATTGCAC AATTGTGCAG GCTCTTTGTT TTCTTGACAA ATCCTACACG 
ACGCGGTACC CGCAGATTTC AGCGTATGCT CTTCGCAGTC TGTTGTCCCA AAAGAGTGAA 
AGTCTTATAT TTTATCTCCC ACAAATCGTA CAATTACTTT CTGGTGATGC ATCGGGCGGT 
ATCGCTGTCT TCCTCCGTAA GATGTGCGAG AAGAGTGATA TGTTTACGCA TCAGCTGCTT 
TGGTCCTTGC GGACGGAAGG GGAGGGGGGA GCTGAAATGG CAAGACGATG CGCACAACTT 
GCTTCTGATG TGGAAGAAGG ACTTGTTGCC AAACGGCGGT CGTTTCACTA CGGGGAGTTT 
TCTTTTATTG AAAAGATCAT CTCCGTGTCG GGTGAAATGA GGTCTTTTGA AAAGGCATTG 
CGGAAGGAAA AACTGCTTCT TCGTCTGCAT GATGCGGAGT TTCACGAACC GGTAGAACGG 
CAACATTTGT ACCTTCCCAC AGACACACGC TGGCGCATTG CGGGGATCAT ACCCGAAACA 
GCTGCCGCGA TGCAAAGTGC GGCAAAGTGC CCCATACTGG TCCAGTTTAA ATGCATCCCG 
CGAGATCCGG ATGATTGCGA GGTTGCCCTG AAGAAAACGA ATGAAAAGTC ACCCGCGGAC 
ACAGAGACTA CCATAAAGGC ATGTATCTTC AAGATGGGCG ATGATTGCCG ACAGGATCAA 
ATTGCTTTAC AAATTATTGG TCTCTTTCAG CGCATTTATG ACGCCATTCA GGTGCCATCT 
TACTTGTATC CATATCGGGT AGTGACGACT GGAAGGGACA GCGCTGTCAT TGAGTGTGTT 
CCTCGTGCGA TGAGCCGGGA CCAAATTGGA AAACTTGTAG AAAGCAACCT TGCAGAGTAC 
TTTGTCCAAA CTTACGGTCA CCCAGAGTCT GTTACGTTTC ACCGGGCCAG AGAATGCTTT 
ATACGAAGTA TGGCTTCATA TTCGGTCGCT TCTTTTGTGC TCAATGTTAA GGATCGTCAT 
AACGGCAATC TCATGATTGA TGCTCACGGG CACCTCGTGC ACATTGATTT TGGATTTTTG 
TTTGACTTCT CCCCAGGGGG AGACATTAAT TTCGAGTCGT CACCATTCAA ACTGACGGCG 
GAAATGGTGC AACTTATGGG ATGTCCTGTG GGCCGTGGAA ATGGAACGGT GCAGAGCCAC 
TCCCTATCAA AGGCGTTGGT GGATCCCGAG GCGTACAATT TGTTTAGGGA TTTAACAGTT 
CGCTGTTACC TTGCGGTGCG CCAATACGCC CGTCAGATTT GTGTACTTGT AGAGTTGATG 
CTGGGCAGTG GCTTGCCCTG CTTCAAGCCC AGGAAGACGA TTCGGGATCT CGCATGGCGA 
CTCTCCATGC AGATGAGCGA AGTAGAGGCT GCTGAATTTA TGCTCCGGCG GATTTCAGAA 
TCGAGGGGGA ATCTCCGAAC GGTTCTTTAT GACAGGTACC AAAATTTCGC GGAAGGCATA 
GAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_809016.1
CDS	1..6909
Translation	MDEIPFPTWVRRSPVFAMTRVLGQHLLQQLQPTRVEPTGPVVVETTTTNITTKRAGTASSSPIRRKCGEVTPLNTRSLSNSEYFSPVRLFGLDEQNTGVFVDNVARLNETNTTSNEFGVQQRSCNSTGMRLGDVLSHPGVALLISLSQINELVSQKVGVTVKNAPALVSLMALAVEWRFLPYEYTKGFYSECASFYTLLMRQLGTTKFFPGVDVQGFVASLVFCGMLLLRGNGEGQKDGEKDGEETNDHASRRVHDAYISQSRGRNIGLAGFDGCIIDSIIISCIPSDIDECVENPIALGALNGLAHTTKSEPIFLSSQLLNHLQNLTDAFTNSSRRREPLHKRLLCYLMEAICCARGVNYTRMRNWLRAVLQPDYVLRGLMQKEDLHARVVAAMLTLLTVWIQDSVTTCTVKYKGAGDAQDEISSFFSREECTEADALLESVWSALQEAASRWEDLRKTNVKLYGGNVRIVVKNLLQAELVRAQCYRTISGMYQEQQVQQGDNTWTGSYLPSAEEEDLRLRVIGNIVKFLILPTSMFSFITAKASATRLRQDIAEQCVKVIALTTSAEGKAISLWKNVSTPSTVTKAQYSHNYHINIDGIVRELIHRVLEVGTLETFQATPLDEEGDEGRMAALCLQGLCATSSHVRSFAVTEVLDSVVRLSTVLQSVDAESSSTDKNASISSSRAAPRLAVCFEMMNRLLVSRHLSILKPLTCDALLRFGKVFWDTFEDTLRVHVRSGDLANPTIKFPSATLLMVLESFWGLVQRCHERIRVMTREGLRLVASGDGEAKARLVEAFNAESRVLRVLLRTLLRCMINVAWPGSGILYGKASERGTHHPAVFDVQRIAVEVLSLFLRPLCLLCQLSTLFPVTDIRHLVYHHENAQKLTQLDEGESFLYHYCFQPSNINSSFYAENGMAQFRSCWLMLSYYRLTREALLRTETDATSTASDKLRCQEKGLNSMDWTKCIRLLAASMPPLMHMRSSDYQQTMADIDVLKHRLFSSGDLGDGKFYQKELVRFLQRYCPEAKHVIKRLSFSEAFLINALATLEMLRASCGSISTITHYQHYELREFDASKDIRKIVKHVTKAATARYISALGETLPDFAASIIDKNARQLVLLYGFAIDSVRSSAKEILRNIISSFPAFATSSTALPLLWAVINVLEVGNATEVERFCEHLQFPEAPAVATDPDSLERRNRLQDAVAFAEYWAQAARKFSPLALVEHATLFIVEEEATSGVIGSAAGSRLALRAHNMDTAPGHPNQNYAQILIKRSNAQGQVMAAFRLTPHCGVGVLLLRQLQAMVRDRLGTVTVTAKSHERLFASLVSTVHAVTKVGPCAEDEDCNDTGTEAGEGISHEEPSTRGDQAALRRLSEADTLLCTVAAFLVSARSSPSTQLELLWHLVRGPIFLFSSHYISAAIQGWQWLLFEKPERYAVPLLVHLVEGFIWTIVQRLGLFDGTRPFQADEVESGEKTTSIQCEALYPKEYNVNSPHKLLIEFLSDCYVDIRGPVSFHPSVLLLLHHLAMRVVEAPSRFSTKDSSFSEMMRCALLMSSICHNLQIVNERRLSIGSPAFVASASIGKVRQGWYKCLLQWFHKTPPSWYFTRDPSAAEEELVVLQKLIKVLDADRDALLRSTHGFLDFDPSKIHACTAAKFTLMGSSVRHTLCDMLSDDSVKAEGVIRGVITSERNRLLDLIALMRLLVEHERTRLAVWLRPRVGAGARLGEGSLNGLSEAVWRRHCEAAARNNPAVLLALVARFPSPHLLGSATRHVTTNPEAYCHLPEAVDLYLNEEVLRSGYPALGLFTNCTIVQALCFLDKSYTTRYPQISAYALRSLLSQKSESLIFYLPQIVQLLSGDASGGIAVFLRKMCEKSDMFTHQLLWSLRTEGEGGAEMARRCAQLASDVEEGLVAKRRSFHYGEFSFIEKIISVSGEMRSFEKALRKEKLLLRLHDAEFHEPVERQHLYLPTDTRWRIAGIIPETAAAMQSAAKCPILVQFKCIPRDPDDCEVALKKTNEKSPADTETTIKACIFKMGDDCRQDQIALQIIGLFQRIYDAIQVPSYLYPYRVVTTGRDSAVIECVPRAMSRDQIGKLVESNLAEYFVQTYGHPESVTFHRARECFIRSMASYSVASFVLNVKDRHNGNLMIDAHGHLVHIDFGFLFDFSPGGDINFESSPFKLTAEMVQLMGCPVGRGNGTVQSHSLSKALVDPEAYNLFRDLTVRCYLAVRQYARQICVLVELMLGSGLPCFKPRKTIRDLAWRLSMQMSEVEAAEFMLRRISESRGNLRTVLYDRYQNFAEGIEM

Target ORF information:

RefSeq Version	XM_803923.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener phosphatidylinositol-kinase domain protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_803923.1

ORF Insert Sequence:

ATGGACGAAA TCCCATTTCC CACATGGGTG CGGCGTTCGC CGGTCTTTGC AATGACGCGG 
GTGCTGGGCC AACACCTGCT GCAACAGTTG CAACCCACGC GTGTAGAACC AACAGGACCA 
GTCGTGGTGG AAACTACAAC CACAAACATC ACAACAAAAA GGGCAGGGAC TGCGTCGTCG 
TCGCCCATAC GGCGAAAATG CGGGGAGGTT ACTCCTCTCA ACACGAGGTC TCTTAGCAAC 
TCCGAATACT TTTCTCCAGT AAGATTATTT GGCTTAGACG AGCAAAATAC AGGGGTTTTT 
GTAGACAATG TTGCGCGGCT TAACGAAACA AACACAACTT CTAACGAGTT TGGGGTGCAG 
CAGCGCTCTT GCAACTCCAC GGGGATGCGG TTGGGCGACG TTCTGAGCCA CCCGGGCGTT 
GCGCTACTGA TTAGTTTGTC GCAGATCAAC GAGCTCGTGT CACAAAAGGT CGGGGTGACT 
GTCAAGAACG CCCCAGCTTT GGTTTCCCTC ATGGCTCTTG CAGTTGAGTG GCGTTTTTTG 
CCGTATGAAT ACACCAAAGG GTTTTATTCT GAGTGCGCCT CCTTTTACAC ATTGTTGATG 
CGTCAGCTTG GAACCACAAA GTTTTTCCCA GGTGTTGACG TGCAGGGATT TGTGGCGTCG 
CTTGTATTTT GTGGGATGCT GCTGCTGCGG GGCAACGGAG AGGGGCAGAA GGACGGTGAA 
AAGGATGGGG AGGAGACGAA TGATCATGCC AGCCGCCGTG TCCACGATGC GTACATCAGC 
CAGAGCAGGG GGAGAAATAT TGGTCTGGCA GGGTTTGATG GCTGCATCAT CGACTCCATC 
ATCATTTCCT GCATTCCATC CGATATTGAC GAGTGCGTTG AGAACCCCAT TGCTCTTGGT 
GCTCTGAATG GACTCGCCCA CACAACGAAA AGTGAACCCA TTTTTCTGAG CTCGCAGCTG 
CTGAATCATT TACAAAATTT GACAGACGCC TTTACAAATT CATCCAGGCG AAGGGAACCG 
TTGCACAAGC GGCTTCTTTG TTACCTCATG GAGGCGATTT GCTGCGCTCG AGGTGTAAAT 
TACACGCGCA TGCGCAACTG GCTTCGGGCC GTGCTTCAAC CGGATTACGT ATTACGAGGC 
CTCATGCAGA AAGAAGACCT GCATGCGCGC GTGGTCGCCG CGATGCTGAC GCTCCTGACG 
GTGTGGATTC AAGACTCCGT AACAACATGT ACGGTAAAAT ACAAAGGTGC GGGTGATGCT 
CAAGACGAAA TCTCTTCATT TTTCTCCCGC GAAGAATGCA CAGAGGCTGA TGCACTTCTA 
GAGAGTGTTT GGTCTGCCCT CCAAGAGGCT GCGAGCCGAT GGGAGGATCT TCGAAAGACA 
AACGTCAAAC TCTACGGTGG TAACGTGCGC ATTGTCGTGA AAAATTTATT ACAGGCAGAA 
TTGGTTCGTG CGCAGTGCTA CAGAACCATT AGTGGCATGT ATCAAGAGCA ACAAGTGCAG 
CAGGGGGATA ATACCTGGAC AGGAAGTTAT TTGCCTTCAG CCGAGGAGGA AGATCTCCGC 
CTCCGTGTGA TTGGAAACAT CGTTAAATTT CTTATCCTTC CGACGAGCAT GTTTAGTTTC 
ATAACGGCGA AGGCGTCAGC GACCCGACTT CGACAGGACA TCGCCGAGCA ATGCGTAAAG 
GTCATTGCGT TGACCACTTC TGCAGAGGGC AAAGCTATCT CTCTTTGGAA AAACGTCTCC 
ACACCCTCTA CTGTGACGAA GGCACAGTAT TCACACAACT ACCACATCAA TATTGATGGC 
ATCGTCCGCG AGTTGATCCA CCGCGTACTG GAGGTTGGAA CTTTGGAGAC TTTTCAAGCC 
ACGCCTCTGG ATGAAGAAGG GGATGAAGGT CGCATGGCGG CATTATGTCT TCAGGGACTT 
TGTGCCACAA GTTCCCATGT GCGGTCCTTT GCGGTGACGG AGGTGTTGGA TTCCGTTGTG 
CGTCTCTCAA CGGTGCTCCA GTCTGTTGAT GCCGAGAGTA GCAGCACGGA TAAAAACGCA 
TCGATTTCGT CAAGCCGAGC TGCTCCGCGT TTAGCAGTGT GCTTTGAGAT GATGAATCGT 
CTGCTTGTGT CGCGGCACCT TTCCATTTTG AAGCCGCTCA CTTGTGATGC CTTGTTACGC 
TTTGGAAAAG TTTTTTGGGA CACGTTCGAG GACACCCTGC GAGTTCATGT GCGTTCTGGC 
GATCTCGCCA ACCCCACGAT AAAGTTTCCC AGTGCCACGC TGCTGATGGT TTTGGAATCC 
TTCTGGGGAC TTGTGCAGCG TTGTCACGAA CGCATTCGTG TCATGACACG GGAGGGTCTT 
CGTCTTGTCG CTTCTGGGGA TGGGGAGGCA AAGGCACGCC TGGTTGAAGC GTTTAACGCG 
GAGAGCCGCG TCCTCCGTGT ACTTTTGCGG ACTTTACTGC GTTGCATGAT TAATGTTGCG 
TGGCCCGGGT CTGGCATTCT CTACGGTAAA GCCTCCGAGA GGGGCACTCA TCATCCGGCC 
GTGTTTGATG TGCAACGTAT TGCCGTTGAG GTGCTGTCAC TATTTCTCCG TCCCCTCTGC 
TTGCTATGCC AGCTTTCCAC ACTGTTTCCC GTGACGGATA TCCGACACCT TGTGTATCAC 
CATGAAAATG CACAAAAGCT CACGCAGTTG GATGAAGGGG AGTCTTTCTT GTACCACTAC 
TGTTTTCAAC CATCTAATAT CAACTCTTCT TTCTATGCCG AAAATGGGAT GGCACAGTTC 
CGTTCTTGTT GGCTCATGTT ATCATACTAC CGTCTTACAA GAGAAGCACT CTTGCGGACC 
GAAACGGATG CGACGAGTAC TGCCTCAGAC AAACTGCGCT GCCAGGAAAA GGGTTTAAAT 
TCAATGGATT GGACCAAATG TATACGATTG CTTGCCGCGT CCATGCCGCC TCTTATGCAT 
ATGCGATCCT CCGATTATCA GCAAACCATG GCAGACATAG ATGTTTTGAA ACATCGGTTA 
TTCAGCAGTG GTGATTTAGG TGACGGTAAG TTTTATCAGA AGGAGTTGGT GCGTTTTCTT 
CAACGCTACT GTCCAGAGGC CAAACATGTA ATTAAGCGTC TTTCTTTTTC GGAGGCCTTT 
TTGATAAATG CTCTCGCGAC ATTGGAGATG CTACGTGCAT CATGTGGCTC TATCTCCACC 
ATAACGCATT ATCAACATTA TGAGCTACGG GAGTTTGATG CCAGTAAGGA TATTAGAAAA 
ATTGTGAAGC ATGTAACAAA GGCTGCCACG GCACGATATA TCTCTGCTCT CGGAGAAACG 
CTCCCGGATT TTGCGGCGAG TATCATTGAT AAGAATGCGC GGCAGCTTGT TTTGCTCTAC 
GGATTTGCTA TTGACTCTGT ACGGAGTTCT GCAAAGGAGA TTCTTCGCAA TATTATTTCT 
AGCTTTCCCG CCTTTGCGAC AAGCAGCACG GCCCTTCCTC TCTTGTGGGC GGTGATCAAT 
GTGCTGGAGG TAGGGAACGC CACGGAGGTG GAACGTTTCT GTGAGCATCT GCAGTTTCCA 
GAGGCGCCAG CTGTTGCCAC GGATCCTGAT TCTCTTGAAC GGCGAAATCG GCTACAGGAT 
GCTGTGGCAT TTGCGGAGTA TTGGGCCCAG GCGGCGCGTA AATTCTCGCC TTTGGCACTT 
GTGGAGCACG CCACCTTATT TATTGTTGAG GAGGAGGCCA CATCTGGGGT GATCGGCTCT 
GCTGCCGGCT CGAGACTTGC CCTTCGTGCA CACAACATGG ACACTGCACC AGGCCATCCT 
AACCAGAATT ACGCCCAAAT TCTCATTAAG AGGAGCAATG CCCAAGGGCA AGTGATGGCA 
GCCTTTCGTT TGACTCCACA TTGTGGGGTG GGCGTTCTTC TTCTTCGCCA GCTTCAGGCG 
ATGGTGAGGG ACAGGCTTGG AACGGTAACC GTGACCGCAA AGTCCCACGA GCGGCTTTTT 
GCGTCCTTGG TGAGCACCGT TCACGCGGTC ACCAAGGTGG GGCCATGTGC CGAAGACGAA 
GACTGCAACG ACACCGGGAC TGAGGCTGGT GAGGGGATAT CGCATGAAGA ACCATCCACG 
CGCGGCGACC AGGCTGCATT ACGAAGGCTT TCCGAGGCCG ATACGCTTCT TTGCACGGTT 
GCCGCATTCT TGGTCTCCGC ACGGAGTTCT CCTTCGACGC AATTGGAACT TCTGTGGCAT 
CTTGTGCGGG GACCCATTTT TTTGTTTTCA AGTCACTACA TATCGGCTGC CATTCAGGGT 
TGGCAGTGGC TTTTATTTGA GAAGCCCGAG CGCTATGCGG TGCCGTTGTT GGTACATTTG 
GTGGAGGGGT TTATCTGGAC GATTGTGCAG AGACTTGGTC TTTTTGACGG CACCAGACCT 
TTTCAGGCGG ATGAAGTGGA GTCGGGGGAG AAAACGACTT CGATTCAGTG CGAGGCTTTA 
TACCCCAAGG AATATAATGT GAACTCCCCG CATAAACTTC TTATCGAGTT TCTCTCGGAT 
TGCTACGTGG ACATTCGTGG GCCTGTGTCT TTTCATCCGT CTGTGCTTCT TCTCCTGCAC 
CACCTTGCCA TGCGAGTGGT TGAGGCACCA TCCCGATTCT CCACGAAAGA TAGTTCCTTT 
TCGGAAATGA TGCGTTGCGC CCTATTAATG AGTTCCATAT GCCATAATTT GCAGATTGTC 
AATGAACGTC GCTTAAGTAT TGGATCACCC GCTTTTGTGG CATCCGCTTC TATTGGAAAA 
GTTCGTCAAG GCTGGTACAA GTGTCTGTTG CAATGGTTTC ACAAGACTCC GCCTTCGTGG 
TACTTTACGC GAGATCCCTC TGCCGCAGAG GAGGAGTTGG TGGTGCTTCA GAAGCTCATA 
AAAGTACTTG ATGCGGATCG AGATGCGTTG CTGCGTTCTA CGCATGGCTT TCTTGATTTT 
GACCCGTCTA AAATTCATGC TTGTACGGCT GCGAAGTTTA CTCTTATGGG AAGTAGTGTG 
CGTCATACAC TCTGTGACAT GCTCTCGGAC GATTCAGTGA AGGCGGAGGG GGTGATTCGG 
GGGGTGATCA CCAGTGAACG AAACCGCCTT TTGGATCTTA TTGCGCTAAT GAGACTTTTG 
GTGGAGCACG AACGCACACG ACTTGCGGTG TGGCTTCGGC CACGTGTAGG GGCTGGCGCC 
CGGTTGGGGG AGGGGAGTTT GAATGGCCTT TCGGAGGCCG TGTGGAGGAG GCATTGTGAG 
GCGGCTGCGA GGAATAACCC TGCGGTGTTA CTGGCGTTGG TGGCAAGATT TCCCAGTCCA 
CATTTATTGG GGTCCGCCAC GCGGCATGTA ACCACCAACC CCGAAGCCTA TTGCCACCTA 
CCCGAGGCAG TTGATCTTTA CCTTAACGAG GAGGTGCTTA GGAGTGGTTA TCCCGCCCTC 
GGTTTATTTA CAAATTGCAC AATTGTGCAG GCTCTTTGTT TTCTTGACAA ATCCTACACG 
ACGCGGTACC CGCAGATTTC AGCGTATGCT CTTCGCAGTC TGTTGTCCCA AAAGAGTGAA 
AGTCTTATAT TTTATCTCCC ACAAATCGTA CAATTACTTT CTGGTGATGC ATCGGGCGGT 
ATCGCTGTCT TCCTCCGTAA GATGTGCGAG AAGAGTGATA TGTTTACGCA TCAGCTGCTT 
TGGTCCTTGC GGACGGAAGG GGAGGGGGGA GCTGAAATGG CAAGACGATG CGCACAACTT 
GCTTCTGATG TGGAAGAAGG ACTTGTTGCC AAACGGCGGT CGTTTCACTA CGGGGAGTTT 
TCTTTTATTG AAAAGATCAT CTCCGTGTCG GGTGAAATGA GGTCTTTTGA AAAGGCATTG 
CGGAAGGAAA AACTGCTTCT TCGTCTGCAT GATGCGGAGT TTCACGAACC GGTAGAACGG 
CAACATTTGT ACCTTCCCAC AGACACACGC TGGCGCATTG CGGGGATCAT ACCCGAAACA 
GCTGCCGCGA TGCAAAGTGC GGCAAAGTGC CCCATACTGG TCCAGTTTAA ATGCATCCCG 
CGAGATCCGG ATGATTGCGA GGTTGCCCTG AAGAAAACGA ATGAAAAGTC ACCCGCGGAC 
ACAGAGACTA CCATAAAGGC ATGTATCTTC AAGATGGGCG ATGATTGCCG ACAGGATCAA 
ATTGCTTTAC AAATTATTGG TCTCTTTCAG CGCATTTATG ACGCCATTCA GGTGCCATCT 
TACTTGTATC CATATCGGGT AGTGACGACT GGAAGGGACA GCGCTGTCAT TGAGTGTGTT 
CCTCGTGCGA TGAGCCGGGA CCAAATTGGA AAACTTGTAG AAAGCAACCT TGCAGAGTAC 
TTTGTCCAAA CTTACGGTCA CCCAGAGTCT GTTACGTTTC ACCGGGCCAG AGAATGCTTT 
ATACGAAGTA TGGCTTCATA TTCGGTCGCT TCTTTTGTGC TCAATGTTAA GGATCGTCAT 
AACGGCAATC TCATGATTGA TGCTCACGGG CACCTCGTGC ACATTGATTT TGGATTTTTG 
TTTGACTTCT CCCCAGGGGG AGACATTAAT TTCGAGTCGT CACCATTCAA ACTGACGGCG 
GAAATGGTGC AACTTATGGG ATGTCCTGTG GGCCGTGGAA ATGGAACGGT GCAGAGCCAC 
TCCCTATCAA AGGCGTTGGT GGATCCCGAG GCGTACAATT TGTTTAGGGA TTTAACAGTT 
CGCTGTTACC TTGCGGTGCG CCAATACGCC CGTCAGATTT GTGTACTTGT AGAGTTGATG 
CTGGGCAGTG GCTTGCCCTG CTTCAAGCCC AGGAAGACGA TTCGGGATCT CGCATGGCGA 
CTCTCCATGC AGATGAGCGA AGTAGAGGCT GCTGAATTTA TGCTCCGGCG GATTTCAGAA 
TCGAGGGGGA ATCTCCGAAC GGTTCTTTAT GACAGGTACC AAAATTTCGC GGAAGGCATA 
GAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053510003.30 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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