SJAG_00364 cDNA ORF clone, Schizosaccharomyces japonicus yFS275

Gene
Clones
gRNAs

Summary

Gene Symbol	SJAG_00364
Entrez Gene ID	7049511
Full Name	ATR checkpoint kinase Rad3
Gene Type	protein-coding
Organism	Schizosaccharomyces japonicus yFS275

mRNA and Protein(s)

mRNA	Protein	Name
XM_002171612.2	XP_002171648.1	ATR checkpoint kinase Rad3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comparative functional genomics of the fission yeasts.
Rhind N, Chen Z, Yassour M, Thompson DA, Haas BJ, Habib N, Wapinski I, Roy S, Lin MF, Heiman DI, Young SK, Furuya K, Guo Y, Pidoux A, Chen HM, Robbertse B, Goldberg JM, Aoki K, Bayne EH, Berlin AM, Desjardins CA, Dobbs E, Dukaj L, Fan L, FitzGerald MG, French C, Gujja S, Hansen K, Keifenheim D, Levin JZ, Mosher RA, Müller CA, Pfiffner J, Priest M, Russ C, Smialowska A, Swoboda P, Sykes SM, Vaughn M, Vengrova S, Yoder R, Zeng Q, Allshire R, Baulcombe D, Birren BW, Brown W, Ekwall K, Kellis M, Leatherwood J, Levin H, Margalit H, Martienssen R, Nieduszynski CA, Spatafora JW, Friedman N, Dalgaard JZ, Baumann P, Niki H, Regev A, Nusbaum C
Science (New York, N.Y.)332(6032)930-6(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SJAG_00364 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SJAG_00364 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSl01832	XM_002171612.2 Latest version!	Schizosaccharomyces japonicus yFS275 ATR checkpoint kinase Rad3 (SJAG_00364), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SJAG_00364 ( XM_002171612.2 ) cDNA ORF clone, Schizosaccharomyces japonicus yFS275 -> XP_002171648.1 Schizosaccharomyces japonicus yFS275 ATR checkpoint kinase Rad3 (SJAG_00364), mRNA.

Price & Availability↑

CloneID	OSl01832
Clone ID Related Accession (Same CDS sequence)	XM_002171612.2
Accession Version	XM_002171612.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1507564800000
Organism	Schizosaccharomyces japonicus yFS275
Product	ATR checkpoint kinase Rad3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_011627860). On Jan 9, 2015 this sequence version replaced XM_002171612.1.

ATGAATTCGA ATATCAATAC AAAAGCAGAG TCCCTTACTC TGTCACAACA ATCCTTTGAT 
GACAGAAATG CTTTTCAACA ACTTCTAGAC GAAGTTTTGG ATGTTAAAAA GTGCTCAAAT 
TCATTTGCCG ACTTTTCCAA TGCCGAGGTC TCATTGCAAC TTATTCAAGT CCTCGTGGAA 
GCAGGTTTAC TCCCTTTTTT AGACAAAAAG AAGGCGGAAA ACGTGCGGAA TAGTCTATTT 
CTTAGACAAG CAGAGCAATC TCTCGATGCG CTTTTACTTG TTATAACGGC AAAACCGGCA 
CTGCTAAACT CGACGAAGTC ATCCATTGTG TTTCTTGAAT GGCTTTTTCC AAGGACAATT 
TTTCTGTATA GTTTGGAATC GCTCATTCCT ATATATGATA AATTATGTGG GTTTCACTTA 
CAGCTTCTTT ACCTCGTTAA CGAGCATGCA CAGTTATCGA TGCATGCAGT GTGGCTAGAG 
GAAGAATGGA AACGGTACAT CGAGTACAGC TTCGGCGCTT TAGTGAGTCT TAGTAAGTCA 
CAATCTGTGG TACCCTTGTA CTCTGCAGCT CCAAATTTTC TTATGAGCAA TAGAACAAAA 
AAAGATGACT TTCTTATAGC GCTTGACACT CGATCTGCGG CTACATGCTT CTTTTTGCAG 
GCGTCCTCGG TGCTTGCAAA GTATCTTCCC CACTCTCAAT TGAAACCTAC TGATATGTTG 
TACATTCAGC ATTGGGACTG GTTGAAACAA CACGTATTTC ACTTGCTGCT AATGCAAAAG 
CTTAATGAGT TTGAACCTGC AGTTCAACTG AGTATGTATT TTCGAGTACT TGACGTACTG 
GTGTGTTTTT CATTGAAGTA TATGCTTCTG AAAGCTCCGT TATGCATTCG TTCTCGGAAT 
TACATTCTTT CTATAAGCAA AGCCGCGTTT CAACTACGCG AATCCGTAAA AGTCGACGCT 
GAGTTTACTG TCTCAATAAT GTTTGTGTAC TTCTGCGCCT TTCAAACGTT ATATCCCGAA 
CATTTGACTT CTGCCGCATC CGAAATCATG TCTTTGGCGG TTCACTATAA AGAAATGACG 
GAGGATGCTC AAACGGATGC ACGGAAATGT GCGAACATTC TTATGCGCTG TCTAAAGCAC 
AAGGATTCTA ACTTTTTCCA CGCTTTGCAA GCCGAAACAG ATGTCAGTTT GGAAAACCCA 
TTTTGTAAAT ATTTTAACAA CGTTATATTG CTTCGATTTG TACGTTACTT TTATTCAACT 
CTCAGTGATT CACCACTGGG TGAATTTACA AATCTAAAAG CAAACCAAGA CGTCTCTTCC 
TTGTATTCCA TTGGCAAATG GGTATCAAAT CATAATCAGG CTTTTGAAAA GCGGTTTGAA 
GATGAGCTTA AGTCAAAAGT CACATCGTTT CTTGAAAATA CAACTGAACT TCTCGTTGAA 
GACCTTCAAT TGTTTGCTAC AATTCACTGT TCTGAAAAGT TGAATACCTC TTCTAAATAT 
GACACTAAAG TGCATACACA TTATTGTTGG CTCTGTGACT CACCTCAACG GATTAAATTC 
TCAACGAGTG CTGAAACGGA AGTTCTGAGA AAAATTCCTA CAGAACAGAA GATTTTGGAT 
GCGTATTGGC GATGGACAAA TCATTCAAAT ACATTTATTG CTTTGGCAGA CTCTGATTCT 
GGTAAGAGAT GTTTGCAAAT GTTATTTAGT TCTGTCAGAA TGCTTCGTAT GAGTGCAGGC 
CGTATATTAC CACGCTTTTT TTCATCATTT TATACGCATG AAGATATTGT TTCCCCAACA 
GAAAACAGAA TAATGATTTT GGAAACATTA AAAAACCTTG CCTCTGGGTC TCATTCAAAG 
TTCTTGGAAA CTACCATTAT GGCTTGGGCG CATATTGCTG AAGTGGCAGA GGGGGAGGAA 
TTACATTTAA TACTTCTGCA GTTAATTGAG TTTATTGTCG AAGGAGATAC CTTTCATCAA 
GGTGTAGCCG TTAGCTGTCT CCATAACGTT GCCCGAAGAA GGGACGTAAC GATATGGCAA 
CTATTCTCCC CTTACTGGAG AACTGTTTGT GTAATGATCA TTAAAAGTGT TACTCATAAG 
CCTGGCATTG TAAAACTTTT TGCTGAAACT ATGGGCATTA CTGATAATGA CTTCTTGACA 
CGAACTCAAG CCTACACGGT TCCGTATCTT GTTTTAACAA AAAATCGTGC GGTTATTCAA 
AAAATAGCGG AAGCCTCTCA TAATACAGTC TCGTCCTTGT GTTTGTCAAA TATGCCTAAA 
ATACTAGCAA CATTTCTAAC ATCCGAATCG GCAAATGTTG AAGAAAGTGT AATGTTACTA 
TTGACGCTAG CGTCATCGGA TTTTGAAAAA ATTGATCTAT CTCGTTTGCT ACGTTCTGAT 
CCTATTTCTG TTACTGTTGA GCTTTTACAA CTTTGTCATA AAGAAGCTCA AGTAAGTCAA 
GTAATTAATG CGATAACGAA AGTCGCAATA CTTGTTTCGC CCAGTTTAAA TGAAAAAAAT 
GTCAATGAGG AAACGCTTCT TCTGGACTTT TTTAATAATC ATATCCTAGG AATCCTTGCA 
GAGTTCTCTA ACATTATAAA CAACTTAAGA GGGAAAGCTT CTATAAATGA AAAGACACGA 
ACTATTATAG GAATGAAAGA AATGATCCAG TTTGCTGGAA GTGCAACAAA ATTGGGTTTA 
CCTCAAATAA CTTCCTGTCT CCAGAGTGCC TTCCAGGACA ATACCTTGCG ATCCTATGCT 
TTGGAAGCCT GGTTGGCCCT GCTTTTAAGT ATTGACGGAC CCGAATACTT ATCACTTGCA 
AGTCTTTCTC TTGTTTTGTT GCCCTCGATG TATCCTAAGA TAAGCAAAAA AGAGAAAATA 
ATTGTGGTAA AGGTTATAGA TTATATTGCA AAGGATCCAA TGGACAACAT TGAGCAGCTT 
CGGCCGGTTC TTCCAGTCTC TTTTGCAAGT GAGTGCTTTT CTGAAACCAG TCGCGATTCA 
TTTTCAAAAA TTCAGGTTGA CGATTTTCAT TTAAATATTG AAGACATCAT GCAGTGTTGC 
CGTAATGAAC ACGAGTCGGT TTGTCTTTTA GGTTTACAAA AGCTCGCTTC GTATCTTCAG 
ACAAAGGAAG CATTTCTTCA GTCGCTAATA ACAGTTGATG TTATTGATCC TCTTATAAAT 
CGTCTTTTAC GATGTTTACT AGACTGTTGC CTCAAATTTG CACATTCTAA TGATGAAATC 
TCATTTTTGG CAGCCAAAAA TTTGGGTATT TTGGGTGCTA TTGACCCGAA TCGTGCGGAT 
GCTCAGAAAA GAATTGGTGG CTTTTTAATG TTAGATAATT TTGAAAATGG AGAGGAATGT 
TTAAAATTCA TTACTCGTTT TATACAGGAT CAGCTCATTC CTGCTTTTAT TACTACTACG 
GATACAAAGG CTCAAAGCTT TTTAGCTTAT GCCTTGCAGG AACTTCTTCA TTTAGGAGGA 
TTTAGATCTG CTGTTATCGG CTCACAAAAG CGCTCTACAA AAAGTGTGGA AGCTCAATAC 
TGGAATTCAC TTCCAGATTC ATCCAAGCGC GTTCTAATAC CGTTTTTGAA CTCAAAGTAC 
CATCTAACTT CATCTCTCAA GCCAAGAATA TCTTTTCCAA TTTATTCAGA GACGACAACT 
TTCACATATT GGATACAGAG CTTTTGTCTA AAGTTAATGC AGTATTACCA TTCTAAAAAT 
GCTGATCGTA TATTTTCTAT TTGTGCAAAG GTAATCAAGG ATCAAGAAAC TAGCATTTCG 
AGGTTCTTGT TCCCATTCCT ACTCTTAAAC GCTGTTTTAA CCGAGTCCGC TGATGAATTA 
AAACTGATCT CTACGGAATT AAAAATGGTT GTCTTTCAAA AACGACCGGA ACGTACAAAT 
CGAGAAAGCA TGCAACGTTA TACCACTTTT CTTAAGTGCT TCTTCGAAGT TGTTGATTAC 
TTCAACAATT GGCTTAGAGC GAGAAGGCGT AGAAGCTGGG AAGAACGGTC TGCGAAAGCT 
CGACGAGAGA ATCGTTATAT GTCTGTTGAT GATGCCGTTT CTGAGGAAGC TACCGTCGTA 
CGTGTGGATT CTTTCCTTTC CCAGTTTCCC GTAGAAAAAC TTGGTCTCGT CTCATTATTT 
TGTGGTTTTC ATGCGAGAGC ACTTTTCTAC TGGGAACAGC ATTTACGCAT GTCGAAAGAG 
AAAACTCCGA GCTCTGTTAC TGAAAATGAT TATCGAATTT TGCAAGAAAT ATACAGCGGA 
ATCGATGATC CAGATGGGAT TGAAGCAATC TCATTTAATT TTCACAACTA TTCTCTTGAT 
CAACAAATAA TTCTGCATGA AAACTCAGAA AACTGGGATT CTGCTCTCAC TTGTTATGAG 
TTAAGCTATG AGAAACAACC AAAGAATGAT ATTCTAAAGG TTGGAATGCT AAACTGTTTG 
CTGCAAGCAG GCCATTACGA ATCTGTCATC AATAACGCTA AACATATATC GTCTCTAGTT 
GAAGAGCCAT CACGTTCTAC AGTTCTCAAT CTTGCTATTG AAGCTGCCTG GAGTGCATTG 
CTTAAAGACA AATTACAAGA ACTTGTTACA AGTAGTTCGT CCGTTTCATT TGATACAAAG 
GTTGGCCGAT TGTTTTTGAA CTATCTCTCC AACATGCATG ATCAGGCGGA GTTCGACCAG 
CTGATTCGTA CATTATATAC CGATGCTACT ATTGCAATTG CGAATACTGG AGCAAACAGT 
TCATATGATT GCTATGACAT TCTCTCAAAG CTTCATGCTA TTCATGACTT TAGTGTTATT 
TCTAGTTATA CGCTCTCTAA AACACCAATC CCAGAAGATG TAGCGTCTGT TTTTAAGACT 
CGTCTTGAGT GTGTTGTTCC TTATGGAAAG TTTAAGCAGA GAATTCTGTC TGCACAATTG 
GTTGGCTTTA GTAGAGGATC AAAAAACTCT GTGTATCAAG CGCCTACACA TTTGGAAATT 
GCAAAACTTG CTCGAAAAAA TCAGCAAATG CAACGAGCGT ATAATTCAAT TTTGCGCGCT 
ATGAGTTTAA ATAAAGCCAT GGCTGCAGTA GAGCACTCAA GATGGTGGTG GCATCAAGGA 
CAGCGTCGTA AAGCGATTGC TGAATTACAG TCCTCTTTTG ATGCATCTCT TTTTGAGTCT 
GCTGACGTTC TTTCGATGCA TTCAATATCT GCTTTATCAA GTACGTCTTC AAATGAGTCG 
AAGGGTAGCG TTGTCAAAGG AAAAGCGTTG TTGACTCTTA CAAAATGGCT TGGCGAAGCT 
GGTCAACTTG GACTGAAGGA TTTAGAGGTC TATTATTACA AAGCGGTTAA TATATATCCT 
GAATGGGAAA AAAGTCACTA TTACTTAGCT CATCATCGTG TATCGATGCT TGAAGAAGAA 
AAGAAGCTTC CTCCTGGGAA AAAGAGCGAA AAGTTTCTCA CTGGTGAACT GGTAACACGA 
ATTATTAATG AGTATGGAAG ATCTCTTTAC TATGGAACGA AGCATATTTA TGAGAGTATG 
CCCAAACTGT TAACACTTTG GTTGGATTTT GGAGCAGAAG AACTTCAGTT GTCGGAAGAA 
GAGGGCGACA GTTATTTCAG AGAACATATC ATTGCCTCAA GGAAGAAGTC GTTGGAGCTT 
ATAAACTCTA ATATAGTCCG ATTGGGTTTA AAGATACCTC AATATCTATT CCTCACTGCC 
TTATCTCAAA TGATTTCACG TATTTGTCAT CCGAACCAGA AGGTTTATCG AGTGCTGGAA 
CATCTCATCA GTTTAGTCAT TTCTGCATAT CCAAAGGAAA CTCTTTGGCA ACTAATGGCT 
GCTACGAAAT CCACTTCTCA CAAACGGTCT GTGCGAGGAA AAAGCATTTT GAATCTGTTA 
TATTCAAAGA AAAAGAATCA ACCAGAAAAC TTAGAAATGA AGAAACTTTG CCAGTCTGCT 
GTCTGTTTAA CAGACAAGCT TTTGGATCTG TGTAATGTTC GTTTAGACAC GCGGGCTGTG 
AAACTTAGTT TAAAGGGTCA TTTCCGGATT TCGCTTAATT CACCTGTTGA CTTAGTAATT 
CCGGCTCAAT CTTTTATGAA CATAACACTT CCTTCTAAAG GAATTGTATA CCATCAGTAT 
CACCCGTTCC CAAACAACCA ACCAACTATT GAAGGCTTTG AAGATGAAGT TGATATCATG 
AATTCCCTAC AGAAACCCAG GAAGGTGACT ATTCGGGGAA CTAATGGAAA GATGTATCAT 
TTCTTATGTA AACCAAAGGA CGATCTACGG AAAGATGCTC GACTAATGGA ACTTAACAAT 
GTTATTAACA AGATTCTTCG AAAAGATCAA GCAGCTAGTC GAAGAAAGCT AAATATACGA 
ACTTATGTGG TTATCCCTCT TAATGAAGAA TGCGGTTTTA TTGAATGGGT TAATAATACT 
CGCCCTTTCC GTGATATACT CCTAAAAATT TACAAGCAAA AGAATGTACC TATTCCATAT 
CAAGAGCTTC GTGCTAATCT TGATATGGCT TTACAAACAC CTAACCCGGC CAAGATCTTT 
GAAGAGAAAA TCTTACCAAG GTTTCCCTCG GTGTTTCATG AATGGTTTAT GGAAGCATTT 
CCCGAACCCA GCAGTTGGGT AAAAAGTCGG CAGAATTACT CGAGAACTCT TGCTGTAATG 
TCAATGGTGG GTTATGTACT TGGACTTGGT GATAGGCATG GAGAGAACAT TTTGTTCGAT 
GAATTAAACG GAGACGCTAT TCATGTTGAT TTTAATTGTC TTTTCGAAAA GGGATTAACA 
TTTGCAAAAC CAGAAAAGGT ACCATTTAGA CTAACTCATA ATATGGTTGA TGCAATGGGG 
CCAACTGGCT ATGAAGGGTC CTTTAGAAAA TCCAGTGAAA TTACGATGCG TCTTTTACGT 
TCCAATCAAA ATACGCTTAT GAGTGTGTTA GAGTCGTTTT TGTACGACCC TCTTGTTGAA 
TGGAACAGAA GAGGGCAAAA TTCGCTTTCT GGTGGTCTTG AACAAAATGA GGCGACTCAA 
GTTTTGGCAA AGATACGCAG AAAATTGCAA GGTTATATTG GAGAGGAGCC GTTACCCTTG 
TCTGTTGAAG GACAGGTACA AGAGCTTATC AAGGAAGCCG TTAGCTCCGA TAACCTCGTA 
CAGATGTATA TTGGTTGGGC AGCATATTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002171648.1
CDS	512..7684
Translation	MNSNINTKAESLTLSQQSFDDRNAFQQLLDEVLDVKKCSNSFADFSNAEVSLQLIQVLVEAGLLPFLDKKKAENVRNSLFLRQAEQSLDALLLVITAKPALLNSTKSSIVFLEWLFPRTIFLYSLESLIPIYDKLCGFHLQLLYLVNEHAQLSMHAVWLEEEWKRYIEYSFGALVSLSKSQSVVPLYSAAPNFLMSNRTKKDDFLIALDTRSAATCFFLQASSVLAKYLPHSQLKPTDMLYIQHWDWLKQHVFHLLLMQKLNEFEPAVQLSMYFRVLDVLVCFSLKYMLLKAPLCIRSRNYILSISKAAFQLRESVKVDAEFTVSIMFVYFCAFQTLYPEHLTSAASEIMSLAVHYKEMTEDAQTDARKCANILMRCLKHKDSNFFHALQAETDVSLENPFCKYFNNVILLRFVRYFYSTLSDSPLGEFTNLKANQDVSSLYSIGKWVSNHNQAFEKRFEDELKSKVTSFLENTTELLVEDLQLFATIHCSEKLNTSSKYDTKVHTHYCWLCDSPQRIKFSTSAETEVLRKIPTEQKILDAYWRWTNHSNTFIALADSDSGKRCLQMLFSSVRMLRMSAGRILPRFFSSFYTHEDIVSPTENRIMILETLKNLASGSHSKFLETTIMAWAHIAEVAEGEELHLILLQLIEFIVEGDTFHQGVAVSCLHNVARRRDVTIWQLFSPYWRTVCVMIIKSVTHKPGIVKLFAETMGITDNDFLTRTQAYTVPYLVLTKNRAVIQKIAEASHNTVSSLCLSNMPKILATFLTSESANVEESVMLLLTLASSDFEKIDLSRLLRSDPISVTVELLQLCHKEAQVSQVINAITKVAILVSPSLNEKNVNEETLLLDFFNNHILGILAEFSNIINNLRGKASINEKTRTIIGMKEMIQFAGSATKLGLPQITSCLQSAFQDNTLRSYALEAWLALLLSIDGPEYLSLASLSLVLLPSMYPKISKKEKIIVVKVIDYIAKDPMDNIEQLRPVLPVSFASECFSETSRDSFSKIQVDDFHLNIEDIMQCCRNEHESVCLLGLQKLASYLQTKEAFLQSLITVDVIDPLINRLLRCLLDCCLKFAHSNDEISFLAAKNLGILGAIDPNRADAQKRIGGFLMLDNFENGEECLKFITRFIQDQLIPAFITTTDTKAQSFLAYALQELLHLGGFRSAVIGSQKRSTKSVEAQYWNSLPDSSKRVLIPFLNSKYHLTSSLKPRISFPIYSETTTFTYWIQSFCLKLMQYYHSKNADRIFSICAKVIKDQETSISRFLFPFLLLNAVLTESADELKLISTELKMVVFQKRPERTNRESMQRYTTFLKCFFEVVDYFNNWLRARRRRSWEERSAKARRENRYMSVDDAVSEEATVVRVDSFLSQFPVEKLGLVSLFCGFHARALFYWEQHLRMSKEKTPSSVTENDYRILQEIYSGIDDPDGIEAISFNFHNYSLDQQIILHENSENWDSALTCYELSYEKQPKNDILKVGMLNCLLQAGHYESVINNAKHISSLVEEPSRSTVLNLAIEAAWSALLKDKLQELVTSSSSVSFDTKVGRLFLNYLSNMHDQAEFDQLIRTLYTDATIAIANTGANSSYDCYDILSKLHAIHDFSVISSYTLSKTPIPEDVASVFKTRLECVVPYGKFKQRILSAQLVGFSRGSKNSVYQAPTHLEIAKLARKNQQMQRAYNSILRAMSLNKAMAAVEHSRWWWHQGQRRKAIAELQSSFDASLFESADVLSMHSISALSSTSSNESKGSVVKGKALLTLTKWLGEAGQLGLKDLEVYYYKAVNIYPEWEKSHYYLAHHRVSMLEEEKKLPPGKKSEKFLTGELVTRIINEYGRSLYYGTKHIYESMPKLLTLWLDFGAEELQLSEEEGDSYFREHIIASRKKSLELINSNIVRLGLKIPQYLFLTALSQMISRICHPNQKVYRVLEHLISLVISAYPKETLWQLMAATKSTSHKRSVRGKSILNLLYSKKKNQPENLEMKKLCQSAVCLTDKLLDLCNVRLDTRAVKLSLKGHFRISLNSPVDLVIPAQSFMNITLPSKGIVYHQYHPFPNNQPTIEGFEDEVDIMNSLQKPRKVTIRGTNGKMYHFLCKPKDDLRKDARLMELNNVINKILRKDQAASRRKLNIRTYVVIPLNEECGFIEWVNNTRPFRDILLKIYKQKNVPIPYQELRANLDMALQTPNPAKIFEEKILPRFPSVFHEWFMEAFPEPSSWVKSRQNYSRTLAVMSMVGYVLGLGDRHGENILFDELNGDAIHVDFNCLFEKGLTFAKPEKVPFRLTHNMVDAMGPTGYEGSFRKSSEITMRLLRSNQNTLMSVLESFLYDPLVEWNRRGQNSLSGGLEQNEATQVLAKIRRKLQGYIGEEPLPLSVEGQVQELIKEAVSSDNLVQMYIGWAAYF

Target ORF information:

RefSeq Version	XM_002171612.2
Organism	Schizosaccharomyces japonicus yFS275
Definition	Schizosaccharomyces japonicus yFS275 ATR checkpoint kinase Rad3 (SJAG_00364), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002171612.2

ORF Insert Sequence:

ATGAATTCGA ATATCAATAC AAAAGCAGAG TCCCTTACTC TGTCACAACA ATCCTTTGAT 
GACAGAAATG CTTTTCAACA ACTTCTAGAC GAAGTTTTGG ATGTTAAAAA GTGCTCAAAT 
TCATTTGCCG ACTTTTCCAA TGCCGAGGTC TCATTGCAAC TTATTCAAGT CCTCGTGGAA 
GCAGGTTTAC TCCCTTTTTT AGACAAAAAG AAGGCGGAAA ACGTGCGGAA TAGTCTATTT 
CTTAGACAAG CAGAGCAATC TCTCGATGCG CTTTTACTTG TTATAACGGC AAAACCGGCA 
CTGCTAAACT CGACGAAGTC ATCCATTGTG TTTCTTGAAT GGCTTTTTCC AAGGACAATT 
TTTCTGTATA GTTTGGAATC GCTCATTCCT ATATATGATA AATTATGTGG GTTTCACTTA 
CAGCTTCTTT ACCTCGTTAA CGAGCATGCA CAGTTATCGA TGCATGCAGT GTGGCTAGAG 
GAAGAATGGA AACGGTACAT CGAGTACAGC TTCGGCGCTT TAGTGAGTCT TAGTAAGTCA 
CAATCTGTGG TACCCTTGTA CTCTGCAGCT CCAAATTTTC TTATGAGCAA TAGAACAAAA 
AAAGATGACT TTCTTATAGC GCTTGACACT CGATCTGCGG CTACATGCTT CTTTTTGCAG 
GCGTCCTCGG TGCTTGCAAA GTATCTTCCC CACTCTCAAT TGAAACCTAC TGATATGTTG 
TACATTCAGC ATTGGGACTG GTTGAAACAA CACGTATTTC ACTTGCTGCT AATGCAAAAG 
CTTAATGAGT TTGAACCTGC AGTTCAACTG AGTATGTATT TTCGAGTACT TGACGTACTG 
GTGTGTTTTT CATTGAAGTA TATGCTTCTG AAAGCTCCGT TATGCATTCG TTCTCGGAAT 
TACATTCTTT CTATAAGCAA AGCCGCGTTT CAACTACGCG AATCCGTAAA AGTCGACGCT 
GAGTTTACTG TCTCAATAAT GTTTGTGTAC TTCTGCGCCT TTCAAACGTT ATATCCCGAA 
CATTTGACTT CTGCCGCATC CGAAATCATG TCTTTGGCGG TTCACTATAA AGAAATGACG 
GAGGATGCTC AAACGGATGC ACGGAAATGT GCGAACATTC TTATGCGCTG TCTAAAGCAC 
AAGGATTCTA ACTTTTTCCA CGCTTTGCAA GCCGAAACAG ATGTCAGTTT GGAAAACCCA 
TTTTGTAAAT ATTTTAACAA CGTTATATTG CTTCGATTTG TACGTTACTT TTATTCAACT 
CTCAGTGATT CACCACTGGG TGAATTTACA AATCTAAAAG CAAACCAAGA CGTCTCTTCC 
TTGTATTCCA TTGGCAAATG GGTATCAAAT CATAATCAGG CTTTTGAAAA GCGGTTTGAA 
GATGAGCTTA AGTCAAAAGT CACATCGTTT CTTGAAAATA CAACTGAACT TCTCGTTGAA 
GACCTTCAAT TGTTTGCTAC AATTCACTGT TCTGAAAAGT TGAATACCTC TTCTAAATAT 
GACACTAAAG TGCATACACA TTATTGTTGG CTCTGTGACT CACCTCAACG GATTAAATTC 
TCAACGAGTG CTGAAACGGA AGTTCTGAGA AAAATTCCTA CAGAACAGAA GATTTTGGAT 
GCGTATTGGC GATGGACAAA TCATTCAAAT ACATTTATTG CTTTGGCAGA CTCTGATTCT 
GGTAAGAGAT GTTTGCAAAT GTTATTTAGT TCTGTCAGAA TGCTTCGTAT GAGTGCAGGC 
CGTATATTAC CACGCTTTTT TTCATCATTT TATACGCATG AAGATATTGT TTCCCCAACA 
GAAAACAGAA TAATGATTTT GGAAACATTA AAAAACCTTG CCTCTGGGTC TCATTCAAAG 
TTCTTGGAAA CTACCATTAT GGCTTGGGCG CATATTGCTG AAGTGGCAGA GGGGGAGGAA 
TTACATTTAA TACTTCTGCA GTTAATTGAG TTTATTGTCG AAGGAGATAC CTTTCATCAA 
GGTGTAGCCG TTAGCTGTCT CCATAACGTT GCCCGAAGAA GGGACGTAAC GATATGGCAA 
CTATTCTCCC CTTACTGGAG AACTGTTTGT GTAATGATCA TTAAAAGTGT TACTCATAAG 
CCTGGCATTG TAAAACTTTT TGCTGAAACT ATGGGCATTA CTGATAATGA CTTCTTGACA 
CGAACTCAAG CCTACACGGT TCCGTATCTT GTTTTAACAA AAAATCGTGC GGTTATTCAA 
AAAATAGCGG AAGCCTCTCA TAATACAGTC TCGTCCTTGT GTTTGTCAAA TATGCCTAAA 
ATACTAGCAA CATTTCTAAC ATCCGAATCG GCAAATGTTG AAGAAAGTGT AATGTTACTA 
TTGACGCTAG CGTCATCGGA TTTTGAAAAA ATTGATCTAT CTCGTTTGCT ACGTTCTGAT 
CCTATTTCTG TTACTGTTGA GCTTTTACAA CTTTGTCATA AAGAAGCTCA AGTAAGTCAA 
GTAATTAATG CGATAACGAA AGTCGCAATA CTTGTTTCGC CCAGTTTAAA TGAAAAAAAT 
GTCAATGAGG AAACGCTTCT TCTGGACTTT TTTAATAATC ATATCCTAGG AATCCTTGCA 
GAGTTCTCTA ACATTATAAA CAACTTAAGA GGGAAAGCTT CTATAAATGA AAAGACACGA 
ACTATTATAG GAATGAAAGA AATGATCCAG TTTGCTGGAA GTGCAACAAA ATTGGGTTTA 
CCTCAAATAA CTTCCTGTCT CCAGAGTGCC TTCCAGGACA ATACCTTGCG ATCCTATGCT 
TTGGAAGCCT GGTTGGCCCT GCTTTTAAGT ATTGACGGAC CCGAATACTT ATCACTTGCA 
AGTCTTTCTC TTGTTTTGTT GCCCTCGATG TATCCTAAGA TAAGCAAAAA AGAGAAAATA 
ATTGTGGTAA AGGTTATAGA TTATATTGCA AAGGATCCAA TGGACAACAT TGAGCAGCTT 
CGGCCGGTTC TTCCAGTCTC TTTTGCAAGT GAGTGCTTTT CTGAAACCAG TCGCGATTCA 
TTTTCAAAAA TTCAGGTTGA CGATTTTCAT TTAAATATTG AAGACATCAT GCAGTGTTGC 
CGTAATGAAC ACGAGTCGGT TTGTCTTTTA GGTTTACAAA AGCTCGCTTC GTATCTTCAG 
ACAAAGGAAG CATTTCTTCA GTCGCTAATA ACAGTTGATG TTATTGATCC TCTTATAAAT 
CGTCTTTTAC GATGTTTACT AGACTGTTGC CTCAAATTTG CACATTCTAA TGATGAAATC 
TCATTTTTGG CAGCCAAAAA TTTGGGTATT TTGGGTGCTA TTGACCCGAA TCGTGCGGAT 
GCTCAGAAAA GAATTGGTGG CTTTTTAATG TTAGATAATT TTGAAAATGG AGAGGAATGT 
TTAAAATTCA TTACTCGTTT TATACAGGAT CAGCTCATTC CTGCTTTTAT TACTACTACG 
GATACAAAGG CTCAAAGCTT TTTAGCTTAT GCCTTGCAGG AACTTCTTCA TTTAGGAGGA 
TTTAGATCTG CTGTTATCGG CTCACAAAAG CGCTCTACAA AAAGTGTGGA AGCTCAATAC 
TGGAATTCAC TTCCAGATTC ATCCAAGCGC GTTCTAATAC CGTTTTTGAA CTCAAAGTAC 
CATCTAACTT CATCTCTCAA GCCAAGAATA TCTTTTCCAA TTTATTCAGA GACGACAACT 
TTCACATATT GGATACAGAG CTTTTGTCTA AAGTTAATGC AGTATTACCA TTCTAAAAAT 
GCTGATCGTA TATTTTCTAT TTGTGCAAAG GTAATCAAGG ATCAAGAAAC TAGCATTTCG 
AGGTTCTTGT TCCCATTCCT ACTCTTAAAC GCTGTTTTAA CCGAGTCCGC TGATGAATTA 
AAACTGATCT CTACGGAATT AAAAATGGTT GTCTTTCAAA AACGACCGGA ACGTACAAAT 
CGAGAAAGCA TGCAACGTTA TACCACTTTT CTTAAGTGCT TCTTCGAAGT TGTTGATTAC 
TTCAACAATT GGCTTAGAGC GAGAAGGCGT AGAAGCTGGG AAGAACGGTC TGCGAAAGCT 
CGACGAGAGA ATCGTTATAT GTCTGTTGAT GATGCCGTTT CTGAGGAAGC TACCGTCGTA 
CGTGTGGATT CTTTCCTTTC CCAGTTTCCC GTAGAAAAAC TTGGTCTCGT CTCATTATTT 
TGTGGTTTTC ATGCGAGAGC ACTTTTCTAC TGGGAACAGC ATTTACGCAT GTCGAAAGAG 
AAAACTCCGA GCTCTGTTAC TGAAAATGAT TATCGAATTT TGCAAGAAAT ATACAGCGGA 
ATCGATGATC CAGATGGGAT TGAAGCAATC TCATTTAATT TTCACAACTA TTCTCTTGAT 
CAACAAATAA TTCTGCATGA AAACTCAGAA AACTGGGATT CTGCTCTCAC TTGTTATGAG 
TTAAGCTATG AGAAACAACC AAAGAATGAT ATTCTAAAGG TTGGAATGCT AAACTGTTTG 
CTGCAAGCAG GCCATTACGA ATCTGTCATC AATAACGCTA AACATATATC GTCTCTAGTT 
GAAGAGCCAT CACGTTCTAC AGTTCTCAAT CTTGCTATTG AAGCTGCCTG GAGTGCATTG 
CTTAAAGACA AATTACAAGA ACTTGTTACA AGTAGTTCGT CCGTTTCATT TGATACAAAG 
GTTGGCCGAT TGTTTTTGAA CTATCTCTCC AACATGCATG ATCAGGCGGA GTTCGACCAG 
CTGATTCGTA CATTATATAC CGATGCTACT ATTGCAATTG CGAATACTGG AGCAAACAGT 
TCATATGATT GCTATGACAT TCTCTCAAAG CTTCATGCTA TTCATGACTT TAGTGTTATT 
TCTAGTTATA CGCTCTCTAA AACACCAATC CCAGAAGATG TAGCGTCTGT TTTTAAGACT 
CGTCTTGAGT GTGTTGTTCC TTATGGAAAG TTTAAGCAGA GAATTCTGTC TGCACAATTG 
GTTGGCTTTA GTAGAGGATC AAAAAACTCT GTGTATCAAG CGCCTACACA TTTGGAAATT 
GCAAAACTTG CTCGAAAAAA TCAGCAAATG CAACGAGCGT ATAATTCAAT TTTGCGCGCT 
ATGAGTTTAA ATAAAGCCAT GGCTGCAGTA GAGCACTCAA GATGGTGGTG GCATCAAGGA 
CAGCGTCGTA AAGCGATTGC TGAATTACAG TCCTCTTTTG ATGCATCTCT TTTTGAGTCT 
GCTGACGTTC TTTCGATGCA TTCAATATCT GCTTTATCAA GTACGTCTTC AAATGAGTCG 
AAGGGTAGCG TTGTCAAAGG AAAAGCGTTG TTGACTCTTA CAAAATGGCT TGGCGAAGCT 
GGTCAACTTG GACTGAAGGA TTTAGAGGTC TATTATTACA AAGCGGTTAA TATATATCCT 
GAATGGGAAA AAAGTCACTA TTACTTAGCT CATCATCGTG TATCGATGCT TGAAGAAGAA 
AAGAAGCTTC CTCCTGGGAA AAAGAGCGAA AAGTTTCTCA CTGGTGAACT GGTAACACGA 
ATTATTAATG AGTATGGAAG ATCTCTTTAC TATGGAACGA AGCATATTTA TGAGAGTATG 
CCCAAACTGT TAACACTTTG GTTGGATTTT GGAGCAGAAG AACTTCAGTT GTCGGAAGAA 
GAGGGCGACA GTTATTTCAG AGAACATATC ATTGCCTCAA GGAAGAAGTC GTTGGAGCTT 
ATAAACTCTA ATATAGTCCG ATTGGGTTTA AAGATACCTC AATATCTATT CCTCACTGCC 
TTATCTCAAA TGATTTCACG TATTTGTCAT CCGAACCAGA AGGTTTATCG AGTGCTGGAA 
CATCTCATCA GTTTAGTCAT TTCTGCATAT CCAAAGGAAA CTCTTTGGCA ACTAATGGCT 
GCTACGAAAT CCACTTCTCA CAAACGGTCT GTGCGAGGAA AAAGCATTTT GAATCTGTTA 
TATTCAAAGA AAAAGAATCA ACCAGAAAAC TTAGAAATGA AGAAACTTTG CCAGTCTGCT 
GTCTGTTTAA CAGACAAGCT TTTGGATCTG TGTAATGTTC GTTTAGACAC GCGGGCTGTG 
AAACTTAGTT TAAAGGGTCA TTTCCGGATT TCGCTTAATT CACCTGTTGA CTTAGTAATT 
CCGGCTCAAT CTTTTATGAA CATAACACTT CCTTCTAAAG GAATTGTATA CCATCAGTAT 
CACCCGTTCC CAAACAACCA ACCAACTATT GAAGGCTTTG AAGATGAAGT TGATATCATG 
AATTCCCTAC AGAAACCCAG GAAGGTGACT ATTCGGGGAA CTAATGGAAA GATGTATCAT 
TTCTTATGTA AACCAAAGGA CGATCTACGG AAAGATGCTC GACTAATGGA ACTTAACAAT 
GTTATTAACA AGATTCTTCG AAAAGATCAA GCAGCTAGTC GAAGAAAGCT AAATATACGA 
ACTTATGTGG TTATCCCTCT TAATGAAGAA TGCGGTTTTA TTGAATGGGT TAATAATACT 
CGCCCTTTCC GTGATATACT CCTAAAAATT TACAAGCAAA AGAATGTACC TATTCCATAT 
CAAGAGCTTC GTGCTAATCT TGATATGGCT TTACAAACAC CTAACCCGGC CAAGATCTTT 
GAAGAGAAAA TCTTACCAAG GTTTCCCTCG GTGTTTCATG AATGGTTTAT GGAAGCATTT 
CCCGAACCCA GCAGTTGGGT AAAAAGTCGG CAGAATTACT CGAGAACTCT TGCTGTAATG 
TCAATGGTGG GTTATGTACT TGGACTTGGT GATAGGCATG GAGAGAACAT TTTGTTCGAT 
GAATTAAACG GAGACGCTAT TCATGTTGAT TTTAATTGTC TTTTCGAAAA GGGATTAACA 
TTTGCAAAAC CAGAAAAGGT ACCATTTAGA CTAACTCATA ATATGGTTGA TGCAATGGGG 
CCAACTGGCT ATGAAGGGTC CTTTAGAAAA TCCAGTGAAA TTACGATGCG TCTTTTACGT 
TCCAATCAAA ATACGCTTAT GAGTGTGTTA GAGTCGTTTT TGTACGACCC TCTTGTTGAA 
TGGAACAGAA GAGGGCAAAA TTCGCTTTCT GGTGGTCTTG AACAAAATGA GGCGACTCAA 
GTTTTGGCAA AGATACGCAG AAAATTGCAA GGTTATATTG GAGAGGAGCC GTTACCCTTG 
TCTGTTGAAG GACAGGTACA AGAGCTTATC AAGGAAGCCG TTAGCTCCGA TAACCTCGTA 
CAGATGTATA TTGGTTGGGC AGCATATTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative functional genomics of the fission yeasts.
Science (New York, N.Y.)332(6032)930-6(2011 May)
Rhind N,Chen Z,Yassour M,Thompson DA,Haas BJ,Habib N,Wapinski I,Roy S,Lin MF,Heiman DI,Young SK,Furuya K,Guo Y,Pidoux A,Chen HM,Robbertse B,Goldberg JM,Aoki K,Bayne EH,Berlin AM,Desjardins CA,Dobbs E,Dukaj L,Fan L,FitzGerald MG,French C,Gujja S,Hansen K,Keifenheim D,Levin JZ,Mosher RA,Müller CA,Pfiffner J,Priest M,Russ C,Smialowska A,Swoboda P,Sykes SM,Vaughn M,Vengrova S,Yoder R,Zeng Q,Allshire R,Baulcombe D,Birren BW,Brown W,Ekwall K,Kellis M,Leatherwood J,Levin H,Margalit H,Martienssen R,Nieduszynski CA,Spatafora JW,Friedman N,Dalgaard JZ,Baumann P,Niki H,Regev A,Nusbaum C

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SJAG_00364 cDNA ORF clone, Schizosaccharomyces japonicus yFS275

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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