ORF » Species Summary » Mus musculus » MID2 cDNA ORF clone

MID2 cDNA ORF clone, Mus musculus(House mouse)

The following MID2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu08971 NM_011845.3
Latest version!		 Mus musculus midline 2 (Mid2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu56503 NM_001358367.1
Latest version!		 Mus musculus midline 2 (Mid2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu56504 NM_001358366.1
Latest version!		 Mus musculus midline 2 (Mid2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu08971 XM_017318482.1
Latest version!		 Mus musculus midline 2 (Mid2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu56503 XM_006528522.3
Latest version!		 Mus musculus midline 2 (Mid2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu56504 XM_006528523.3
Latest version!		 Mus musculus midline 2 (Mid2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu56505 XM_006528524.4
Latest version!		 Mus musculus midline 2 (Mid2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OMu56505 XM_006528524.3 Mus musculus midline 2 (Mid2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu08971 NM_011845.2 Mus musculus midline 2 (Mid2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    MID2 ( NM_011845.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_035975.1 Mus musculus midline 2 (Mid2), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OMu08971
    Clone ID Related Accession (Same CDS sequence) NM_011845.3 , XM_017318482.1 , NM_011845.2
    Accession Version NM_011845.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1571587200000
    Organism Mus musculus(house mouse)
    Product probable E3 ubiquitin-protein ligase MID2 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL683809.7, AF196480.1 and BX470203.6. On or before Dec 1, 2017 this sequence version replaced XM_017318482.1, NM_011845.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.231121.1, SRR1660821.60243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
    CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
    TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
    ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
    AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
    AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
    CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
    TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
    AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
    TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
    TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
    GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
    ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_035975.1
    CDS188..2245
    Translation

    Target ORF information:

    RefSeq Version NM_011845.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 2 (Mid2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_011845.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
    CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
    TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
    ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
    AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
    AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
    CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
    TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
    AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
    TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
    TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
    GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
    ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID2 ( NM_011845.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_035975.1 Mus musculus midline 2 (Mid2), mRNA.
    Price & Availability↑
    CloneID OMu08971
    Clone ID Related Accession (Same CDS sequence) NM_011845.3 , XM_017318482.1 , NM_011845.2
    Accession Version NM_011845.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1493308800000
    Organism Mus musculus(House mouse)
    Product probable E3 ubiquitin-protein ligase MID2
    Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF196480.1 and BX470203.6. On Mar 22, 2007 this sequence version replaced NM_011845.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by mRNA and EST alignments. ##Evidence-Data-START## Transcript exon combination :: AF196480.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
    CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
    TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
    ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
    AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
    AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
    CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
    TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
    AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
    TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
    TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
    GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
    ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_035975.1
    CDS25..2082
    Translation

    Target ORF information:

    RefSeq Version NM_011845.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus midline 2 (Mid2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_011845.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
    CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
    TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
    ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
    AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
    AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
    CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
    TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
    AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
    TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
    TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
    GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
    ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID2 ( NM_001358367.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001345296.1 Mus musculus midline 2 (Mid2), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OMu56503
    Clone ID Related Accession (Same CDS sequence) NM_001358367.1 , XM_006528522.3
    Accession Version NM_001358367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1571587200000
    Organism Mus musculus(house mouse)
    Product probable E3 ubiquitin-protein ligase MID2 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL683809.7 and BX470203.6. On Dec 1, 2017 this sequence version replaced XM_006528522.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
    TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
    CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
    AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
    AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
    GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
    ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
    AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
    AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
    GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
    GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
    CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
    AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001345296.1
    CDS320..2527
    Translation

    Target ORF information:

    RefSeq Version NM_001358367.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 2 (Mid2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001358367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
    TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
    CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
    AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
    AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
    GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
    ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
    AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
    AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
    GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
    GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
    CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
    AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID2 ( NM_001358366.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001345295.1 Mus musculus midline 2 (Mid2), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OMu56504
    Clone ID Related Accession (Same CDS sequence) NM_001358366.1 , XM_006528523.3
    Accession Version NM_001358366.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1571673600000
    Organism Mus musculus(house mouse)
    Product probable E3 ubiquitin-protein ligase MID2 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL683809.7 and BX470203.6. On Dec 1, 2017 this sequence version replaced XM_006528523.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: Y18881.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
    CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
    TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
    ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
    AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
    AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
    CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
    TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
    AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
    TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
    TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
    GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
    ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001345295.1
    CDS320..2437
    Translation

    Target ORF information:

    RefSeq Version NM_001358366.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 2 (Mid2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001358366.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
    CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
    TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
    ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
    AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
    AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
    CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
    TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
    AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
    TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
    TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
    GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
    ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID2 ( XM_017318482.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017173971.1 Mus musculus midline 2 (Mid2), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OMu08971
    Clone ID Related Accession (Same CDS sequence) NM_011845.3 , XM_017318482.1 , NM_011845.2
    Accession Version XM_017318482.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466438400000
    Organism Mus musculus(House mouse)
    Product probable E3 ubiquitin-protein ligase MID2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
    CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
    TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
    ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
    AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
    AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
    CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
    TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
    AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
    TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
    TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
    GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
    ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173971.1
    CDS396..2453
    Translation

    Target ORF information:

    RefSeq Version XM_017318482.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus midline 2 (Mid2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017318482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
    CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
    TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
    ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
    AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
    AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
    CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
    TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
    AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
    TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
    TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
    GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
    ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID2 ( XM_006528522.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006528585.1 Mus musculus midline 2 (Mid2), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OMu56503
    Clone ID Related Accession (Same CDS sequence) NM_001358367.1 , XM_006528522.3
    Accession Version XM_006528522.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466438400000
    Organism Mus musculus(House mouse)
    Product probable E3 ubiquitin-protein ligase MID2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528522.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
    TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
    CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
    AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
    AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
    GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
    ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
    AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
    AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
    GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
    GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
    CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
    AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006528585.1
    CDS602..2809
    Translation

    Target ORF information:

    RefSeq Version XM_006528522.3
    Organism Mus musculus(House mouse)
    Definition Mus musculus midline 2 (Mid2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006528522.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
    TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
    CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
    AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
    AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
    GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
    ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
    AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
    AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
    GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
    GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
    CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
    AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID2 ( XM_006528523.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006528586.1 Mus musculus midline 2 (Mid2), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OMu56504
    Clone ID Related Accession (Same CDS sequence) NM_001358366.1 , XM_006528523.3
    Accession Version XM_006528523.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466438400000
    Organism Mus musculus(House mouse)
    Product probable E3 ubiquitin-protein ligase MID2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528523.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
    CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
    TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
    ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
    AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
    AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
    CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
    TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
    AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
    TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
    TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
    GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
    ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006528586.1
    CDS600..2717
    Translation

    Target ORF information:

    RefSeq Version XM_006528523.3
    Organism Mus musculus(House mouse)
    Definition Mus musculus midline 2 (Mid2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006528523.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
    CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
    TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
    ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
    AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
    AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
    CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
    TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
    AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
    TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
    TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
    GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
    ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID2 ( XM_006528524.4 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006528587.1 Mus musculus midline 2 (Mid2), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OMu56505
    Clone ID Related Accession (Same CDS sequence) XM_006528524.4 , XM_006528524.3
    Accession Version XM_006528524.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1565107200000
    Organism Mus musculus(house mouse)
    Product probable E3 ubiquitin-protein ligase MID2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528524.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
    TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
    CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
    AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
    AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
    GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
    ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
    AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
    AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
    GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
    GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
    CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
    AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006528587.1
    CDS396..2543
    Translation

    Target ORF information:

    RefSeq Version XM_006528524.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 2 (Mid2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006528524.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
    TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
    CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
    AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
    AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
    GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
    ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
    AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
    AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
    GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
    GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
    CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
    AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID2 ( XM_006528524.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006528587.1 Mus musculus midline 2 (Mid2), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OMu56505
    Clone ID Related Accession (Same CDS sequence) XM_006528524.4 , XM_006528524.3
    Accession Version XM_006528524.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466438400000
    Organism Mus musculus(house mouse)
    Product probable E3 ubiquitin-protein ligase MID2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528524.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
    TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
    CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
    AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
    AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
    GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
    ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
    AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
    AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
    GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
    GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
    CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
    AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006528587.1
    CDS252..2399
    Translation

    Target ORF information:

    RefSeq Version XM_006528524.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 2 (Mid2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006528524.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
    CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
    TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
    AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
    AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
    GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
    GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
    TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
    CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
    AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
    AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
    GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
    ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
    AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
    AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
    GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
    GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
    CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
    AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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