LMJF_36_2080 cDNA ORF clone, Leishmania major strain Friedlin

Gene
Clones
gRNAs

Summary

Gene Symbol	LMJF_36_2080
Entrez Gene ID	5655466
Full Name	streptococcal hemagglutinin-like protein
Gene Type	protein-coding
Organism	Leishmania major strain Friedlin

mRNA and Protein(s)

mRNA	Protein	Name
XM_001686732.1	XP_001686784.1	streptococcal hemagglutinin-like protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

The genome of the kinetoplastid parasite, Leishmania major.
Ivens AC, Peacock CS, Worthey EA, Murphy L, Aggarwal G, Berriman M, Sisk E, Rajandream MA, Adlem E, Aert R, Anupama A, Apostolou Z, Attipoe P, Bason N, Bauser C, Beck A, Beverley SM, Bianchettin G, Borzym K, Bothe G, Bruschi CV, Collins M, Cadag E, Ciarloni L, Clayton C, Coulson RM, Cronin A, Cruz AK, Davies RM, De Gaudenzi J, Dobson DE, Duesterhoeft A, Fazelina G, Fosker N, Frasch AC, Fraser A, Fuchs M, Gabel C, Goble A, Goffeau A, Harris D, Hertz-Fowler C, Hilbert H, Horn D, Huang Y, Klages S, Knights A, Kube M, Larke N, Litvin L, Lord A, Louie T, Marra M, Masuy D, Matthews K, Michaeli S, Mottram JC, Müller-Auer S, Munden H, Nelson S, Norbertczak H, Oliver K, O'neil S, Pentony M, Pohl TM, Price C, Purnelle B, Quail MA, Rabbinowitsch E, Reinhardt R, Rieger M, Rinta J, Robben J, Robertson L, Ruiz JC, Rutter S, Saunders D, Schäfer M, Schein J, Schwartz DC, Seeger K, Seyler A, Sharp S, Shin H, Sivam D, Squares R, Squares S, Tosato V, Vogt C, Volckaert G, Wambutt R, Warren T, Wedler H, Woodward J, Zhou S, Zimmermann W, Smith DF, Blackwell JM, Stuart KD, Barrell B, Myler PJ
Science (New York, N.Y.)309(5733)436-42(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LMJF_36_2080 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_36_2080 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLb05794	XM_001686732.1 Latest version!	Leishmania major strain Friedlin streptococcal hemagglutinin-like protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LMJF_36_2080 ( XM_001686732.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001686784.1 Leishmania major strain Friedlin streptococcal hemagglutinin-like protein partial mRNA.

Price & Availability↑

CloneID	OLb05794
Clone ID Related Accession (Same CDS sequence)	XM_001686732.1
Accession Version	XM_001686732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497456000000
Organism	Leishmania major strain Friedlin
Product	streptococcal hemagglutinin-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007287). COMPLETENESS: incomplete on both ends.

ATGCGTATCA CCGCGGCGAC AGCGACCGCC CCTGTCGGTG CCCCAGGCGC CAAAGTGCGC 
AAGGGAAAAG CAAAGACCAT CCGCGCCGTC GCCGCGGCAG GCGCAGTATG GCGCCCCCAA 
TCGCAGCTCA TCTTCACCTC CAACGCCACC TCTGTGACGG CACCGTCGAA CGGCGAACTT 
GGTGACTCGA AAACCGCAGG GGCCATCAGC ACCAATGGCA GCATCAACGG CTACACCGGC 
ACCTCATCAG CGCTGGCGCT AAAGGCTGCA GGTACTGGAG GCAGTGGCAG CTGTGGGGTT 
GCCTCGGTGC TCCCCATACC CACCGCGCCA TCCTCTGGTA GCGCGCAAGT GCCCCCGCTG 
CCGTCGTCAG GGTGCGCTGC GGTGGAAAAC CAGATTTTGC GGAACGACGC TCTCGAAGCG 
GTGAGGCGAT CAGGCGCCGA GGGTTTTCCA CCCTCGCGGG AGAAGGGCGT TGTCAGTTGC 
ACGTTGCCAG CAGCCGAAAA GCAACGCCGT GTCTCGGACA TTGCCAGGGC AAATGAGCTC 
AGCACAGACA ACCCCAATAC GTTCACCCCT GCTGAGGTGC CGCCTTGGGT GGCTGGCGGC 
GCCCCTGTCG ATGCTTCCAA GGCTGCAGAC ACCGGTGGGG GCGCCAGCGC TGCTTCTTTC 
GAAGGAGTGA TGGACAACTC GCCCTTCGTG TCGAGCTCCT CCTTGGCCCC TTCCGACACA 
GGATGTACGC AGCGGACAAA GGCCTCGCAC GCAGACCAGC GGCCCTCAGC GACGCAACGG 
TGCGACAACG GCGCTTTCGC GGAGCGGCCG CAGCTGCTCC TGTCGCTCGC GCAGGACAAC 
CGGGGCTTCC TCAATCCTGG CTACTCGTGC GACTCCTCAG ACTGGAGCAG TGAGGACAGC 
GACGCTAGCA GTGGGCGTGC AGGTGCTGAC GGCAGCGCCG GCTCGGCAAG GCGCCAGCGC 
ACCCCATCTG CGGCACCTTC CAGTCGAGCC GCACCATCGG AGCAGCGCGG AAAAGCGTGC 
GGCGGCGCGC CTCCGCCGGT GCTCACTACG CGTTTGAGTA GCGGCAGGAA CCGGACCGCC 
CTCGCTGTCT TCCCGCTGAC CGCCGCAGAG GAGCAGTCAG CCCGGCAAGC GTACGACACC 
TACCGAGCAC AGCAAGAGAA AGCGGGCTAT CACAGGAGCG ACACCAGCGC CACTAAGCTC 
CGCTCTCAGC GCTCTGCTTC CGTGTCCCCA TCCGCCCTGA GCACTTCCAC GTCTGTAGGG 
AAGCGTGAGC TGCTGCGGCT ACTTCACGGG CTTTATCACC GTGCACAGGC CATGATGGCC 
TCTACCACAA AGACCGCCGC GCCGACGTCC GCCCCGCCGC AGAGGAAGGC GATGTCGCCA 
ACCATGAGCG TTGGCACGGC AGACCTGCTG CACAAGTTTC CGTCAACCAA GTACCTGCTC 
AACGCGGCTG AGGCATGCTT TGGCAGCCGT CGTTTCCGAA CTCTCATGGC CGCTGTGGTG 
GCAGACAGTG ACGCCCTCGT CCCCATCGTC GTTCGAAACA ACGACGGAGG AGCGTCAGCC 
GTGCCCACCG CTTCACGGTC ACGGTTGTCG TCTTCGTCTC AAGGATTGAC AGTGGAGGAG 
GCGCTCTCCT TCTTTAGGCA CCTCAAACAT GAAATGACCT CGGATGTGGC TAGGCACCGG 
GAGAGGAGGC AGGCAGAGGC GTCCGCGAAG CCAGAAGCGG CGTCGGGGGA CAAAAGACGG 
AATCGCCCAG CGAAGGCTGC TCCATTACAG CCCCTACTTG ACCTGCAGGG CTCGCAGAAG 
ACGCGTAGTA CTTCTGCCCC CAGTTGTCAA GCTGCCTCCC AGGTGTGCCG TCTCCCTTCT 
GCGCAGGCCG GTCGACGCAG CGGCCGACCT CCGCCGACTC CTATTGCGCT GATGGCGGCG 
AGGCATGTCA CGTCGGCATC TTTCCCGCCG TCGTCTACCC CACCTTCGCC TTCTCCGCAG 
TTGTCTTCCT CACCGTCGAG GCCAGGGATT CCCGGGGCGA TGACGTTGGT GGGCCCTCGG 
AAGCCAACTG GACGGCGTAA GGGCCCGCGC AGAAAAACAG CGCTGCCGCC GCCGTCGCCC 
CATCCGTTCG GTAGCAACTC GTGCACGCCC GACGCAACTT ACATGGATGA CTTCATCCAC 
ACATATGTGC AGCGCAAGGC GTTTGCTGAG ATCGCCGAAG GACAGGGCTT CCACGACAGC 
ACCACCGTGA CATTGGGGAA GCTGATAAAA GTGCTACACT GGTTCGAGCT GGCGCTCGAC 
CCCAGCGTCA TCGAGCACCT GTGCGGCGTG CAGGTGCAGC CCGCGCGAGA ATCGCCGGAC 
ATCTCCATTG ACTTCAGTAC CTTTGTGAAA ATCATCAACT CGGGCTTGTG GTGCCAGCGC 
TACAGTGGGG GCAGCGGCTC TTCAGCACCC GGCTCCACGT CGGCGGACAA CAACGATGAT 
CCGGAGTCCA ACGCGTGTGT GCCGGTGTTG CCACCAGAGG CGCCTGCACG GGCCTCTCCT 
GTGGCGCTGC CTATTCCTGG CTTGACGGCG GCCGACAGTG CATCGCTCAG CACGGGTGGC 
GGGGGTGCAC CTTGCGCTAA GGCTGCCTCC TCCACCATCT CAGTGCGTGA CATTGGGGGC 
TCGAATCCGT CGCCACTGGT CCTTGCCCCA GTTTCTGCTG CGGCGCATCG TCGATTGAGC 
AGCCTCAGCA GCATGTCGAC TTCAACCGCT TCTTCACCGG CAAGTGGCGG CAGCATGCAC 
AGATCATTTG TGGACACAGA TGATGCGTAC CCATTGGTAG CTCATGACTC CGCCATCGCA 
GCGCTCTGCC GCAGTATTCG CCAGCGGCTG CGCCGAGAGC TGCGGCGGAG CGTGAGCAGT 
GGGTCTAACG CGCTGCTGCG ACCCCGAGAC AACCAAGGCA GCGGCAACGG GGCTGGCGAT 
GGCGCGGCGC AAAAGGAAGG AAGCGACCCA CAGTATTCTC CATCTAGCCG GACGGCGCCG 
GCTTCCTCGT GCTCGTGGAG GTCGTTGTCT TTGGCCTCCT CCGCAGCGTC GTCAATGTCC 
AGGGGGAACG TGAGTGTCGC CGGTCTGGTT GGTGAGAGCG AATCCGCTTC TGCGCGTGAC 
GATGGCGAGG TATTCTCGGC ATCGCCACAC TTTTCAAAGT GGCGCAGTGA CACGAAGTGC 
AGCACTCCGC TGTCTCACCA GAGGCTGCGC GAGCAGCAGT CGCCGTCGCC GCATGTAGGT 
CATAGGCTGC CTCCTCACTC GCATCCGTAC ACGCTGACCC CTACCCCAAG AGCCCCGCGT 
GCACAGCCCG CCAGAAGAGG TGAGGGCGCC GTTGCCTCCC AGTGGTTCCC GACAGCGCAG 
CGTTGGCAGG GGCACGGGCA CCGACTCGCT GCTCCGCCGC CCGCCAGGCC GGCTACTGCG 
CAAAGACCAT CTCTCTCCAC TGCCGTGGCG AGGCGGTGTC GCCGGCATCA AGAATCAATG 
CACTTTATCA CGTCCCTGTC GCCTTACAGC AATTCAGGCC ATGGCGCCCG ACGGTCCGCA 
AGCCATTGCT GGTCGCGCAT CGCGACTCTA TCAGCGCTGA GTCGGTCGCG AAGGTCGTCG 
TCGCTGCACT CCTGTGACAC CCAGTCAGTG GTGAATTCGT TCCAATTTTC AGAGTCCGGC 
TTGTCTGCCG CTTTCTTGTC CCGGACTTTG CCTGCACAGA CGGTGTCGAG GCACCCCAAA 
CGGAACCGTC CATCTAGCGC CTATACGCGA CTCACGACGG CACCAACGCA TGACGCCTGC 
ACAGCGCACG TGTCACACCC CCCTCTCTCA CCAGCCATCA GATCGTCGCC GCTGCCGTAT 
CGGCGGCGGC TCTTCCAGGC CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001686784.1
CDS	1..3867
Translation	MRITAATATAPVGAPGAKVRKGKAKTIRAVAAAGAVWRPQSQLIFTSNATSVTAPSNGELGDSKTAGAISTNGSINGYTGTSSALALKAAGTGGSGSCGVASVLPIPTAPSSGSAQVPPLPSSGCAAVENQILRNDALEAVRRSGAEGFPPSREKGVVSCTLPAAEKQRRVSDIARANELSTDNPNTFTPAEVPPWVAGGAPVDASKAADTGGGASAASFEGVMDNSPFVSSSSLAPSDTGCTQRTKASHADQRPSATQRCDNGAFAERPQLLLSLAQDNRGFLNPGYSCDSSDWSSEDSDASSGRAGADGSAGSARRQRTPSAAPSSRAAPSEQRGKACGGAPPPVLTTRLSSGRNRTALAVFPLTAAEEQSARQAYDTYRAQQEKAGYHRSDTSATKLRSQRSASVSPSALSTSTSVGKRELLRLLHGLYHRAQAMMASTTKTAAPTSAPPQRKAMSPTMSVGTADLLHKFPSTKYLLNAAEACFGSRRFRTLMAAVVADSDALVPIVVRNNDGGASAVPTASRSRLSSSSQGLTVEEALSFFRHLKHEMTSDVARHRERRQAEASAKPEAASGDKRRNRPAKAAPLQPLLDLQGSQKTRSTSAPSCQAASQVCRLPSAQAGRRSGRPPPTPIALMAARHVTSASFPPSSTPPSPSPQLSSSPSRPGIPGAMTLVGPRKPTGRRKGPRRKTALPPPSPHPFGSNSCTPDATYMDDFIHTYVQRKAFAEIAEGQGFHDSTTVTLGKLIKVLHWFELALDPSVIEHLCGVQVQPARESPDISIDFSTFVKIINSGLWCQRYSGGSGSSAPGSTSADNNDDPESNACVPVLPPEAPARASPVALPIPGLTAADSASLSTGGGGAPCAKAASSTISVRDIGGSNPSPLVLAPVSAAAHRRLSSLSSMSTSTASSPASGGSMHRSFVDTDDAYPLVAHDSAIAALCRSIRQRLRRELRRSVSSGSNALLRPRDNQGSGNGAGDGAAQKEGSDPQYSPSSRTAPASSCSWRSLSLASSAASSMSRGNVSVAGLVGESESASARDDGEVFSASPHFSKWRSDTKCSTPLSHQRLREQQSPSPHVGHRLPPHSHPYTLTPTPRAPRAQPARRGEGAVASQWFPTAQRWQGHGHRLAAPPPARPATAQRPSLSTAVARRCRRHQESMHFITSLSPYSNSGHGARRSASHCWSRIATLSALSRSRRSSSLHSCDTQSVVNSFQFSESGLSAAFLSRTLPAQTVSRHPKRNRPSSAYTRLTTAPTHDACTAHVSHPPLSPAIRSSPLPYRRRLFQAC

Target ORF information:

RefSeq Version	XM_001686732.1
Organism	Leishmania major strain Friedlin
Definition	Leishmania major strain Friedlin streptococcal hemagglutinin-like protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001686732.1

ORF Insert Sequence:

ATGCGTATCA CCGCGGCGAC AGCGACCGCC CCTGTCGGTG CCCCAGGCGC CAAAGTGCGC 
AAGGGAAAAG CAAAGACCAT CCGCGCCGTC GCCGCGGCAG GCGCAGTATG GCGCCCCCAA 
TCGCAGCTCA TCTTCACCTC CAACGCCACC TCTGTGACGG CACCGTCGAA CGGCGAACTT 
GGTGACTCGA AAACCGCAGG GGCCATCAGC ACCAATGGCA GCATCAACGG CTACACCGGC 
ACCTCATCAG CGCTGGCGCT AAAGGCTGCA GGTACTGGAG GCAGTGGCAG CTGTGGGGTT 
GCCTCGGTGC TCCCCATACC CACCGCGCCA TCCTCTGGTA GCGCGCAAGT GCCCCCGCTG 
CCGTCGTCAG GGTGCGCTGC GGTGGAAAAC CAGATTTTGC GGAACGACGC TCTCGAAGCG 
GTGAGGCGAT CAGGCGCCGA GGGTTTTCCA CCCTCGCGGG AGAAGGGCGT TGTCAGTTGC 
ACGTTGCCAG CAGCCGAAAA GCAACGCCGT GTCTCGGACA TTGCCAGGGC AAATGAGCTC 
AGCACAGACA ACCCCAATAC GTTCACCCCT GCTGAGGTGC CGCCTTGGGT GGCTGGCGGC 
GCCCCTGTCG ATGCTTCCAA GGCTGCAGAC ACCGGTGGGG GCGCCAGCGC TGCTTCTTTC 
GAAGGAGTGA TGGACAACTC GCCCTTCGTG TCGAGCTCCT CCTTGGCCCC TTCCGACACA 
GGATGTACGC AGCGGACAAA GGCCTCGCAC GCAGACCAGC GGCCCTCAGC GACGCAACGG 
TGCGACAACG GCGCTTTCGC GGAGCGGCCG CAGCTGCTCC TGTCGCTCGC GCAGGACAAC 
CGGGGCTTCC TCAATCCTGG CTACTCGTGC GACTCCTCAG ACTGGAGCAG TGAGGACAGC 
GACGCTAGCA GTGGGCGTGC AGGTGCTGAC GGCAGCGCCG GCTCGGCAAG GCGCCAGCGC 
ACCCCATCTG CGGCACCTTC CAGTCGAGCC GCACCATCGG AGCAGCGCGG AAAAGCGTGC 
GGCGGCGCGC CTCCGCCGGT GCTCACTACG CGTTTGAGTA GCGGCAGGAA CCGGACCGCC 
CTCGCTGTCT TCCCGCTGAC CGCCGCAGAG GAGCAGTCAG CCCGGCAAGC GTACGACACC 
TACCGAGCAC AGCAAGAGAA AGCGGGCTAT CACAGGAGCG ACACCAGCGC CACTAAGCTC 
CGCTCTCAGC GCTCTGCTTC CGTGTCCCCA TCCGCCCTGA GCACTTCCAC GTCTGTAGGG 
AAGCGTGAGC TGCTGCGGCT ACTTCACGGG CTTTATCACC GTGCACAGGC CATGATGGCC 
TCTACCACAA AGACCGCCGC GCCGACGTCC GCCCCGCCGC AGAGGAAGGC GATGTCGCCA 
ACCATGAGCG TTGGCACGGC AGACCTGCTG CACAAGTTTC CGTCAACCAA GTACCTGCTC 
AACGCGGCTG AGGCATGCTT TGGCAGCCGT CGTTTCCGAA CTCTCATGGC CGCTGTGGTG 
GCAGACAGTG ACGCCCTCGT CCCCATCGTC GTTCGAAACA ACGACGGAGG AGCGTCAGCC 
GTGCCCACCG CTTCACGGTC ACGGTTGTCG TCTTCGTCTC AAGGATTGAC AGTGGAGGAG 
GCGCTCTCCT TCTTTAGGCA CCTCAAACAT GAAATGACCT CGGATGTGGC TAGGCACCGG 
GAGAGGAGGC AGGCAGAGGC GTCCGCGAAG CCAGAAGCGG CGTCGGGGGA CAAAAGACGG 
AATCGCCCAG CGAAGGCTGC TCCATTACAG CCCCTACTTG ACCTGCAGGG CTCGCAGAAG 
ACGCGTAGTA CTTCTGCCCC CAGTTGTCAA GCTGCCTCCC AGGTGTGCCG TCTCCCTTCT 
GCGCAGGCCG GTCGACGCAG CGGCCGACCT CCGCCGACTC CTATTGCGCT GATGGCGGCG 
AGGCATGTCA CGTCGGCATC TTTCCCGCCG TCGTCTACCC CACCTTCGCC TTCTCCGCAG 
TTGTCTTCCT CACCGTCGAG GCCAGGGATT CCCGGGGCGA TGACGTTGGT GGGCCCTCGG 
AAGCCAACTG GACGGCGTAA GGGCCCGCGC AGAAAAACAG CGCTGCCGCC GCCGTCGCCC 
CATCCGTTCG GTAGCAACTC GTGCACGCCC GACGCAACTT ACATGGATGA CTTCATCCAC 
ACATATGTGC AGCGCAAGGC GTTTGCTGAG ATCGCCGAAG GACAGGGCTT CCACGACAGC 
ACCACCGTGA CATTGGGGAA GCTGATAAAA GTGCTACACT GGTTCGAGCT GGCGCTCGAC 
CCCAGCGTCA TCGAGCACCT GTGCGGCGTG CAGGTGCAGC CCGCGCGAGA ATCGCCGGAC 
ATCTCCATTG ACTTCAGTAC CTTTGTGAAA ATCATCAACT CGGGCTTGTG GTGCCAGCGC 
TACAGTGGGG GCAGCGGCTC TTCAGCACCC GGCTCCACGT CGGCGGACAA CAACGATGAT 
CCGGAGTCCA ACGCGTGTGT GCCGGTGTTG CCACCAGAGG CGCCTGCACG GGCCTCTCCT 
GTGGCGCTGC CTATTCCTGG CTTGACGGCG GCCGACAGTG CATCGCTCAG CACGGGTGGC 
GGGGGTGCAC CTTGCGCTAA GGCTGCCTCC TCCACCATCT CAGTGCGTGA CATTGGGGGC 
TCGAATCCGT CGCCACTGGT CCTTGCCCCA GTTTCTGCTG CGGCGCATCG TCGATTGAGC 
AGCCTCAGCA GCATGTCGAC TTCAACCGCT TCTTCACCGG CAAGTGGCGG CAGCATGCAC 
AGATCATTTG TGGACACAGA TGATGCGTAC CCATTGGTAG CTCATGACTC CGCCATCGCA 
GCGCTCTGCC GCAGTATTCG CCAGCGGCTG CGCCGAGAGC TGCGGCGGAG CGTGAGCAGT 
GGGTCTAACG CGCTGCTGCG ACCCCGAGAC AACCAAGGCA GCGGCAACGG GGCTGGCGAT 
GGCGCGGCGC AAAAGGAAGG AAGCGACCCA CAGTATTCTC CATCTAGCCG GACGGCGCCG 
GCTTCCTCGT GCTCGTGGAG GTCGTTGTCT TTGGCCTCCT CCGCAGCGTC GTCAATGTCC 
AGGGGGAACG TGAGTGTCGC CGGTCTGGTT GGTGAGAGCG AATCCGCTTC TGCGCGTGAC 
GATGGCGAGG TATTCTCGGC ATCGCCACAC TTTTCAAAGT GGCGCAGTGA CACGAAGTGC 
AGCACTCCGC TGTCTCACCA GAGGCTGCGC GAGCAGCAGT CGCCGTCGCC GCATGTAGGT 
CATAGGCTGC CTCCTCACTC GCATCCGTAC ACGCTGACCC CTACCCCAAG AGCCCCGCGT 
GCACAGCCCG CCAGAAGAGG TGAGGGCGCC GTTGCCTCCC AGTGGTTCCC GACAGCGCAG 
CGTTGGCAGG GGCACGGGCA CCGACTCGCT GCTCCGCCGC CCGCCAGGCC GGCTACTGCG 
CAAAGACCAT CTCTCTCCAC TGCCGTGGCG AGGCGGTGTC GCCGGCATCA AGAATCAATG 
CACTTTATCA CGTCCCTGTC GCCTTACAGC AATTCAGGCC ATGGCGCCCG ACGGTCCGCA 
AGCCATTGCT GGTCGCGCAT CGCGACTCTA TCAGCGCTGA GTCGGTCGCG AAGGTCGTCG 
TCGCTGCACT CCTGTGACAC CCAGTCAGTG GTGAATTCGT TCCAATTTTC AGAGTCCGGC 
TTGTCTGCCG CTTTCTTGTC CCGGACTTTG CCTGCACAGA CGGTGTCGAG GCACCCCAAA 
CGGAACCGTC CATCTAGCGC CTATACGCGA CTCACGACGG CACCAACGCA TGACGCCTGC 
ACAGCGCACG TGTCACACCC CCCTCTCTCA CCAGCCATCA GATCGTCGCC GCTGCCGTAT 
CGGCGGCGGC TCTTCCAGGC CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

The genome of the kinetoplastid parasite, Leishmania major.
Science (New York, N.Y.)309(5733)436-42(2005 Jul)
Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ

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LMJF_36_2080 cDNA ORF clone, Leishmania major strain Friedlin

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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