LMJF_33_0950 cDNA ORF clone, Leishmania major strain Friedlin

Gene
Clones
gRNAs

Summary

Gene Symbol	LMJF_33_0950
Entrez Gene ID	5654506
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Leishmania major strain Friedlin

mRNA and Protein(s)

mRNA	Protein	Name
XM_001685800.1	XP_001685852.1	conserved hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

The genome of the kinetoplastid parasite, Leishmania major.
Ivens AC, Peacock CS, Worthey EA, Murphy L, Aggarwal G, Berriman M, Sisk E, Rajandream MA, Adlem E, Aert R, Anupama A, Apostolou Z, Attipoe P, Bason N, Bauser C, Beck A, Beverley SM, Bianchettin G, Borzym K, Bothe G, Bruschi CV, Collins M, Cadag E, Ciarloni L, Clayton C, Coulson RM, Cronin A, Cruz AK, Davies RM, De Gaudenzi J, Dobson DE, Duesterhoeft A, Fazelina G, Fosker N, Frasch AC, Fraser A, Fuchs M, Gabel C, Goble A, Goffeau A, Harris D, Hertz-Fowler C, Hilbert H, Horn D, Huang Y, Klages S, Knights A, Kube M, Larke N, Litvin L, Lord A, Louie T, Marra M, Masuy D, Matthews K, Michaeli S, Mottram JC, Müller-Auer S, Munden H, Nelson S, Norbertczak H, Oliver K, O'neil S, Pentony M, Pohl TM, Price C, Purnelle B, Quail MA, Rabbinowitsch E, Reinhardt R, Rieger M, Rinta J, Robben J, Robertson L, Ruiz JC, Rutter S, Saunders D, Schäfer M, Schein J, Schwartz DC, Seeger K, Seyler A, Sharp S, Shin H, Sivam D, Squares R, Squares S, Tosato V, Vogt C, Volckaert G, Wambutt R, Warren T, Wedler H, Woodward J, Zhou S, Zimmermann W, Smith DF, Blackwell JM, Stuart KD, Barrell B, Myler PJ
Science (New York, N.Y.)309(5733)436-42(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LMJF_33_0950 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_33_0950 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLb04913	XM_001685800.1 Latest version!	Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LMJF_33_0950 ( XM_001685800.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001685852.1 Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

Price & Availability↑

CloneID	OLb04913
Clone ID Related Accession (Same CDS sequence)	XM_001685800.1
Accession Version	XM_001685800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497456000000
Organism	Leishmania major strain Friedlin
Product	conserved hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007285). COMPLETENESS: incomplete on both ends.

ATGCGCGCCT TCCAAGTGCC CCCGCTCGGG GCAGATGACC AACTGCTGAA AGCCGCATCA 
AGCGAGCGCC CGGGCAGCGG AACCGCTGAC GGTGTTGTAG CGCCACTGAA GACGCACCGC 
TCGGCAAATG AGCTGTCCGC CATCGTGGCT GACCTCCACC GAACCTCTCA TTCAGCGCAC 
CCGCCCGACC AGAAACGCAC AGGTGACCCC ACTGGCGCGC ATTCGCCCGC AGAGGGTAAG 
CAACAGATGC ACGCGCGTGC TCCTGCTCCT CCGCATTCCT CATCGAGGCT AGCCTTGCGG 
AGCTTGTCTC AGGAGGAGTT TGTCCATGCC ATTCAGACCG CAGTGCCGTC TGCCACTCTG 
CCCGAGATCC AGGCGCTGCT CAGCAAGACG ATCTCCGAGA CGCAGGACAC CGTCTCCTGG 
AATGAACTGA CCGCGTTTCT GGTGACCCGC TCGCGACAGA AGGCCGACCT CGCCCTCGAG 
AATCAGCGTT TCGTCCTTGG CGGCCCGCCA TGTGGGATGC GCTTCGACGA CCAGCACAGC 
AGCTCCATCA CCTGCGTTGC GGTGGAGCCG GTGCGCCGCC TTCTCGTGAC GGGCTGCAGT 
GAGGGCTCTG TGCGGGCCTG GAGCAGCGGC AGTGACCTTG CCTACCGAGG CCTACTGCTG 
CAGGTGGATA AGTGGATTGT GGGGCTGCAC TGGGGATGCA ACAAGCGTGT CCTCTATGTC 
GTCACCATGG ATCGCTGGGT TTATATCCTG GACGGAACCA CGTACGAGGT GCTCCGCGTC 
TACCACGGCC GCGGCATCAC TCAGTCCACG GTCAGCATGA CGTACGCGAA CGAGACGATC 
GACACTGTGC ACGTCGGCGG AGTGGCACTA TCGAAATTCG CCGCATCGAG TGCTCGCCGC 
TACGGCAGCG CGCGCCTTAT CCCACAGGGC ATCGGCTCCC CAGATAAATC GAGGACGCCG 
CCGTCATCGC GTCCCCTCAC TAGCCGCGCC GGCGCGATGG AGTCGCATGA GGAGCGCACG 
CGACGTCTGC TCTTCTCTGC CATGCACGTG TGCCGCGCTG TGCGTCCCGC GAGCAAAGAG 
ACGGATGCCG CCGAGCACCC GCACCGCAAC TGCTCACCAC AGACTGGGGA TGCCACCGCG 
GCAGCGGGTG CGAGCGCGTC ACCGCCGTCC CAGTCACGCA CAGAGGCGTC ATCTCCGTTG 
CCTACATCCA CTCTGACGAC GATGTACTCG CTCACAACCG CCGACGCAGC TGAGGTGCTG 
CACCAGAACA ACACCCCGCT AACCGGGGCT GGCAGAGGTC CCTATGTTCA CCAGCAACTG 
GACGAATGCG TTCTCACTGC CTTGATCGAC CCTGTGACGG CGACGGCGTT TCACGAATCT 
GCTTTCCAAG AAGATGTGCT GCTGCTAGGC ACGAGCGCTG GCGACGTCTT TCTCTTTGAG 
CTTGCGCAGC ATCACCACCT CAACGCAAAT CGAGTGCTCG TGGCGCGGCA CGTCTTCCGC 
CAGCTGCACC GCGGGCGCAT CACAAAACTG GATCTTCTGT TGCCTCTGCA CGCCCTAGTC 
AGCTCCGGCG ACGACGGACA CGTCCGCGTC ATGTCCCTTG TAACTGGGGA GCCACTTCGG 
ACCTTCTACG CGGCTGACCT CCCCGAGCAG CACGCAAGCG TAACCGACTT TGACCTGCAC 
CCTCAGCTAA AGATGTTGCT GACTGTCGGC CCAGAGCGGC GTGCGCTCGT GTGGGAATGG 
ACTCAGCCCT CTCCCATCGC ACTGCTCGAC CCGGCCAACA GCCCATGCTG CTGCGGCGCC 
TTCATCGGTG ATCGAGTGCT GACGATTAGC CGAGATGGGG TGCTGCACGT CTACGACTGC 
AAAGGGTTTC ACCTCCAGCA GGAAGTCTCC TTGGCCACGG CGGGCTCCCT CGATCGCTTC 
GGCAGCGCCG TCGGGGCCAC CCACTCCGCC ATCTCGCAGG TGCACGTGGA CGAGGCGCGG 
CAGTGGCTGC TGTGCTTTGG GCACTTCCCG TTTTCCCTGC GCGTAAAGTG GCAAGTCTCC 
TCTGGCTTTC CGGAGCGCTA CCGAGGTCAC CACGTGCCGA TCCTCACGAC GCTGTCTTCG 
CGTGCCTTTC GGCAGGTGGT GACGGTAGGC ACAGACGGTG TCGTCATGAC GTGGACGCCG 
CGGACAGGGG CAAACGAGTT TTCGTTTCCA CTCTCGAACT TCTCGAACAT GGCAACAGCT 
TCAGTTCCGC TTCGACCGAC GGCTGTCTCC ATGGACCTCT TGCAGCGCCG CCTACTCACG 
GGCTTTGCCG ACGGCATCAT GGTTGTATGG AACATCCTGA ACGGCCAAGT CGAGCGCGTC 
TTGACAGCTG CCGCTGCAAC TGCGTTACCG TCGCGTTCGT CCTCGCCGGC GGCTTCGAGG 
GGCACAACGA GGAAGAGAGT GAAGCCGATA TCCGGCCATG GTCGATCTAC CGCCAGGGCC 
TCTGTGGAGC GAGCTGTGGC GACAGCGACA GCGGTGGCGT TGACCTCACC GAGTCGCGAC 
GTCACCGCCG TCGGCTCGTT TCTGCGGCAC CGCTCCGTGT CGTACATCTT CGCCCTTGGC 
TCGCGCGTGT ATGTGGACGC GGCGGTGGAA AGCTCTGGTG CCGCAGAGCG GTGCAGTGGC 
CCTCAACTCG GCGAGTACTC TACCACCCCG GCGTCATCGT GGACGGTGCC GGGTGCATTT 
GGCGATGTGG CAACGCTAGT GCAGCTAGGG ATGCAGCTTG TCGGCTGCGC GACCGCCTCT 
GGAGCAGTTC TCGTGTATAA CGTGCTCTTC GATCGGCAGG AAGGTGCACC GCTGTGGGTG 
CACGAGTCTT TGCTATCCCC GACGTGGGCC ACCGGCAGCG TTCTCTCTCC GACGTCAACG 
GTGAACGCCG CATGCGATTT CGCCAGCGTT GGAAGTTCGA GTACATTACT GTGGAGAAAC 
GGCACCGTGT CGACTGCCGG CCAGCAGATG AACGGCAGCG GCGTGCTTGC CGGAACGCAC 
AGTCAGCGCA CCTGCCCTGG CGCCGCCACG CTCTTCCTCG AGTCCCTTGC GAACAGAGCC 
AGGGCTGCTG GGGCGGCCCA GGCTGCTGGT GCTGACCCGG TGGCCGGGGC GGCAGCGACG 
ACAGGTGCAG TTGTCTCGCG CGTAAGCTGC CTCATGATGC TGCCGGCTGT CCACCCGCGG 
TTGCTGTTCG TGGGACAGGA GGACGGCACC GTGTCGTTCT GGCACACGCT GCGTCGCGTT 
TGCCTGGGCG CCGTGAGTTT GACAACTGCG GCTGGTGTCG AGGATGGGAG GCAAGGGGAG 
GGCACGGAGT GGGGTGGAGG CGAGGCGGTG GTGGGCATGG ACATGGAGGA GACAGACGGG 
CAAATGCTCG TGTTCGGCGA CGGTGAGGGC AAGGTTCACG TATGCCGTTT GAAGTGGAAG 
ATGCTGGCCG ACTTGCATGA GCAGACGGCT GCTCTCGCGA TGCCTAATCT GGCCTTGTAC 
TGCCGGACGC CAACGCCAAG GAAGGAGCAA CTTCCCGCGG TTGCAGCGTC GACGGCGAGC 
TCAGCAGAGG ATGCGACGGC TTCCGAGCGG GCGCTGCCGG CGCTGCAGGA ACTGGAGCGC 
GTGCACGTGT TTTCAAGTGG GCTCAAGCTT TCCGGCGTTC GTCTTGTCCA CGCAGACGAG 
GAGACCGCGG TCTTCCGTGC CGCCGTGCCG CTGCCCAAGA CGAAGACTGG CGCTGAAGAT 
GTTGGTACGG CACCGGAAGA GGGCGGGGGC AGAGACGCAC CCAGTTCCTC GCCGGGGACG 
CCCAGGGATA CGGACAGGCC TCGTCTGCTC ATCATCTGCA CTGGCATGGA CCACTTCGTG 
CGTGTCTTCA CGTTGGCAGG CGTCCCTATT GGAGAACTCG GAATGGACGA GTGGGACACG 
GCGCGGCCGT CGACCTTCCG CTTCATGGGC GAGCCGACCG TCCCGCTGCC GTGCTCGCTG 
GCCGGCAACT TCACGTGGCA GCAAGACGGA CACGACAAAA TCAAGAGGAG CGGCTGCTAC 
CACGATTACT TGGCCGACCT GTACGCGACG CACCATGCAC GACCTGCGAT GCTAACCGGG 
CCTACCAAGG GGCTTGCATG CAGTGGCAAC GTGCCGCCGG GCAGCTCCGC AGCGTTACGA 
CGAACAATGG GCGGAACCGT CTACCTTGGC TGTCCCGACA TCCCCCCATC ACGCGCCACC 
GTCAGGGCGT CGTTGGTGGC CCGTCCGTCG CCTGCTCCCG ACGCCCCGCA CCCAGTGGAC 
AGGGTCAGGA GCGCCGCGGG AGTGGACAAG CGGCAGAGCC ACGGGCACTC AAAAGTCGTC 
GGCTTCACGA AGAACGTCGA AACGGGCACG AGGGCTGCGC CCGAGGTGTT CTCCGAGGAC 
GGTCGCAGTC TTCTCCCGCC TCAGCTGAGC CCCGACCAAC GGCGCCCCGC CAGCCAAGGC 
TTCCACGCAC GCCTCCGGCA AAACATGCTG CATTTGTGCG AGAAAGATCG ACCCCTTCAT 
GGGGTGCTAA AGCACTCGTA TGTACGTCAG CTCCAAAACC TCCACCGCCA GGGGCACCCA 
TCGTCGTTGT CGTCGTTCCA ACAGCCACTG GCAGCGCTAC CCGAGCTGCC TTCCATCCTG 
CCCAGTACCT CCGCGACACC ATCTGCTGCA GCCAGCGAGC ACAGCGAATC CGCTGGACAA 
AGCCAGGCTG CAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001685852.1
CDS	1..4698
Translation	MRAFQVPPLGADDQLLKAASSERPGSGTADGVVAPLKTHRSANELSAIVADLHRTSHSAHPPDQKRTGDPTGAHSPAEGKQQMHARAPAPPHSSSRLALRSLSQEEFVHAIQTAVPSATLPEIQALLSKTISETQDTVSWNELTAFLVTRSRQKADLALENQRFVLGGPPCGMRFDDQHSSSITCVAVEPVRRLLVTGCSEGSVRAWSSGSDLAYRGLLLQVDKWIVGLHWGCNKRVLYVVTMDRWVYILDGTTYEVLRVYHGRGITQSTVSMTYANETIDTVHVGGVALSKFAASSARRYGSARLIPQGIGSPDKSRTPPSSRPLTSRAGAMESHEERTRRLLFSAMHVCRAVRPASKETDAAEHPHRNCSPQTGDATAAAGASASPPSQSRTEASSPLPTSTLTTMYSLTTADAAEVLHQNNTPLTGAGRGPYVHQQLDECVLTALIDPVTATAFHESAFQEDVLLLGTSAGDVFLFELAQHHHLNANRVLVARHVFRQLHRGRITKLDLLLPLHALVSSGDDGHVRVMSLVTGEPLRTFYAADLPEQHASVTDFDLHPQLKMLLTVGPERRALVWEWTQPSPIALLDPANSPCCCGAFIGDRVLTISRDGVLHVYDCKGFHLQQEVSLATAGSLDRFGSAVGATHSAISQVHVDEARQWLLCFGHFPFSLRVKWQVSSGFPERYRGHHVPILTTLSSRAFRQVVTVGTDGVVMTWTPRTGANEFSFPLSNFSNMATASVPLRPTAVSMDLLQRRLLTGFADGIMVVWNILNGQVERVLTAAAATALPSRSSSPAASRGTTRKRVKPISGHGRSTARASVERAVATATAVALTSPSRDVTAVGSFLRHRSVSYIFALGSRVYVDAAVESSGAAERCSGPQLGEYSTTPASSWTVPGAFGDVATLVQLGMQLVGCATASGAVLVYNVLFDRQEGAPLWVHESLLSPTWATGSVLSPTSTVNAACDFASVGSSSTLLWRNGTVSTAGQQMNGSGVLAGTHSQRTCPGAATLFLESLANRARAAGAAQAAGADPVAGAAATTGAVVSRVSCLMMLPAVHPRLLFVGQEDGTVSFWHTLRRVCLGAVSLTTAAGVEDGRQGEGTEWGGGEAVVGMDMEETDGQMLVFGDGEGKVHVCRLKWKMLADLHEQTAALAMPNLALYCRTPTPRKEQLPAVAASTASSAEDATASERALPALQELERVHVFSSGLKLSGVRLVHADEETAVFRAAVPLPKTKTGAEDVGTAPEEGGGRDAPSSSPGTPRDTDRPRLLIICTGMDHFVRVFTLAGVPIGELGMDEWDTARPSTFRFMGEPTVPLPCSLAGNFTWQQDGHDKIKRSGCYHDYLADLYATHHARPAMLTGPTKGLACSGNVPPGSSAALRRTMGGTVYLGCPDIPPSRATVRASLVARPSPAPDAPHPVDRVRSAAGVDKRQSHGHSKVVGFTKNVETGTRAAPEVFSEDGRSLLPPQLSPDQRRPASQGFHARLRQNMLHLCEKDRPLHGVLKHSYVRQLQNLHRQGHPSSLSSFQQPLAALPELPSILPSTSATPSAAASEHSESAGQSQAAE

Target ORF information:

RefSeq Version	XM_001685800.1
Organism	Leishmania major strain Friedlin
Definition	Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001685800.1

ORF Insert Sequence:

ATGCGCGCCT TCCAAGTGCC CCCGCTCGGG GCAGATGACC AACTGCTGAA AGCCGCATCA 
AGCGAGCGCC CGGGCAGCGG AACCGCTGAC GGTGTTGTAG CGCCACTGAA GACGCACCGC 
TCGGCAAATG AGCTGTCCGC CATCGTGGCT GACCTCCACC GAACCTCTCA TTCAGCGCAC 
CCGCCCGACC AGAAACGCAC AGGTGACCCC ACTGGCGCGC ATTCGCCCGC AGAGGGTAAG 
CAACAGATGC ACGCGCGTGC TCCTGCTCCT CCGCATTCCT CATCGAGGCT AGCCTTGCGG 
AGCTTGTCTC AGGAGGAGTT TGTCCATGCC ATTCAGACCG CAGTGCCGTC TGCCACTCTG 
CCCGAGATCC AGGCGCTGCT CAGCAAGACG ATCTCCGAGA CGCAGGACAC CGTCTCCTGG 
AATGAACTGA CCGCGTTTCT GGTGACCCGC TCGCGACAGA AGGCCGACCT CGCCCTCGAG 
AATCAGCGTT TCGTCCTTGG CGGCCCGCCA TGTGGGATGC GCTTCGACGA CCAGCACAGC 
AGCTCCATCA CCTGCGTTGC GGTGGAGCCG GTGCGCCGCC TTCTCGTGAC GGGCTGCAGT 
GAGGGCTCTG TGCGGGCCTG GAGCAGCGGC AGTGACCTTG CCTACCGAGG CCTACTGCTG 
CAGGTGGATA AGTGGATTGT GGGGCTGCAC TGGGGATGCA ACAAGCGTGT CCTCTATGTC 
GTCACCATGG ATCGCTGGGT TTATATCCTG GACGGAACCA CGTACGAGGT GCTCCGCGTC 
TACCACGGCC GCGGCATCAC TCAGTCCACG GTCAGCATGA CGTACGCGAA CGAGACGATC 
GACACTGTGC ACGTCGGCGG AGTGGCACTA TCGAAATTCG CCGCATCGAG TGCTCGCCGC 
TACGGCAGCG CGCGCCTTAT CCCACAGGGC ATCGGCTCCC CAGATAAATC GAGGACGCCG 
CCGTCATCGC GTCCCCTCAC TAGCCGCGCC GGCGCGATGG AGTCGCATGA GGAGCGCACG 
CGACGTCTGC TCTTCTCTGC CATGCACGTG TGCCGCGCTG TGCGTCCCGC GAGCAAAGAG 
ACGGATGCCG CCGAGCACCC GCACCGCAAC TGCTCACCAC AGACTGGGGA TGCCACCGCG 
GCAGCGGGTG CGAGCGCGTC ACCGCCGTCC CAGTCACGCA CAGAGGCGTC ATCTCCGTTG 
CCTACATCCA CTCTGACGAC GATGTACTCG CTCACAACCG CCGACGCAGC TGAGGTGCTG 
CACCAGAACA ACACCCCGCT AACCGGGGCT GGCAGAGGTC CCTATGTTCA CCAGCAACTG 
GACGAATGCG TTCTCACTGC CTTGATCGAC CCTGTGACGG CGACGGCGTT TCACGAATCT 
GCTTTCCAAG AAGATGTGCT GCTGCTAGGC ACGAGCGCTG GCGACGTCTT TCTCTTTGAG 
CTTGCGCAGC ATCACCACCT CAACGCAAAT CGAGTGCTCG TGGCGCGGCA CGTCTTCCGC 
CAGCTGCACC GCGGGCGCAT CACAAAACTG GATCTTCTGT TGCCTCTGCA CGCCCTAGTC 
AGCTCCGGCG ACGACGGACA CGTCCGCGTC ATGTCCCTTG TAACTGGGGA GCCACTTCGG 
ACCTTCTACG CGGCTGACCT CCCCGAGCAG CACGCAAGCG TAACCGACTT TGACCTGCAC 
CCTCAGCTAA AGATGTTGCT GACTGTCGGC CCAGAGCGGC GTGCGCTCGT GTGGGAATGG 
ACTCAGCCCT CTCCCATCGC ACTGCTCGAC CCGGCCAACA GCCCATGCTG CTGCGGCGCC 
TTCATCGGTG ATCGAGTGCT GACGATTAGC CGAGATGGGG TGCTGCACGT CTACGACTGC 
AAAGGGTTTC ACCTCCAGCA GGAAGTCTCC TTGGCCACGG CGGGCTCCCT CGATCGCTTC 
GGCAGCGCCG TCGGGGCCAC CCACTCCGCC ATCTCGCAGG TGCACGTGGA CGAGGCGCGG 
CAGTGGCTGC TGTGCTTTGG GCACTTCCCG TTTTCCCTGC GCGTAAAGTG GCAAGTCTCC 
TCTGGCTTTC CGGAGCGCTA CCGAGGTCAC CACGTGCCGA TCCTCACGAC GCTGTCTTCG 
CGTGCCTTTC GGCAGGTGGT GACGGTAGGC ACAGACGGTG TCGTCATGAC GTGGACGCCG 
CGGACAGGGG CAAACGAGTT TTCGTTTCCA CTCTCGAACT TCTCGAACAT GGCAACAGCT 
TCAGTTCCGC TTCGACCGAC GGCTGTCTCC ATGGACCTCT TGCAGCGCCG CCTACTCACG 
GGCTTTGCCG ACGGCATCAT GGTTGTATGG AACATCCTGA ACGGCCAAGT CGAGCGCGTC 
TTGACAGCTG CCGCTGCAAC TGCGTTACCG TCGCGTTCGT CCTCGCCGGC GGCTTCGAGG 
GGCACAACGA GGAAGAGAGT GAAGCCGATA TCCGGCCATG GTCGATCTAC CGCCAGGGCC 
TCTGTGGAGC GAGCTGTGGC GACAGCGACA GCGGTGGCGT TGACCTCACC GAGTCGCGAC 
GTCACCGCCG TCGGCTCGTT TCTGCGGCAC CGCTCCGTGT CGTACATCTT CGCCCTTGGC 
TCGCGCGTGT ATGTGGACGC GGCGGTGGAA AGCTCTGGTG CCGCAGAGCG GTGCAGTGGC 
CCTCAACTCG GCGAGTACTC TACCACCCCG GCGTCATCGT GGACGGTGCC GGGTGCATTT 
GGCGATGTGG CAACGCTAGT GCAGCTAGGG ATGCAGCTTG TCGGCTGCGC GACCGCCTCT 
GGAGCAGTTC TCGTGTATAA CGTGCTCTTC GATCGGCAGG AAGGTGCACC GCTGTGGGTG 
CACGAGTCTT TGCTATCCCC GACGTGGGCC ACCGGCAGCG TTCTCTCTCC GACGTCAACG 
GTGAACGCCG CATGCGATTT CGCCAGCGTT GGAAGTTCGA GTACATTACT GTGGAGAAAC 
GGCACCGTGT CGACTGCCGG CCAGCAGATG AACGGCAGCG GCGTGCTTGC CGGAACGCAC 
AGTCAGCGCA CCTGCCCTGG CGCCGCCACG CTCTTCCTCG AGTCCCTTGC GAACAGAGCC 
AGGGCTGCTG GGGCGGCCCA GGCTGCTGGT GCTGACCCGG TGGCCGGGGC GGCAGCGACG 
ACAGGTGCAG TTGTCTCGCG CGTAAGCTGC CTCATGATGC TGCCGGCTGT CCACCCGCGG 
TTGCTGTTCG TGGGACAGGA GGACGGCACC GTGTCGTTCT GGCACACGCT GCGTCGCGTT 
TGCCTGGGCG CCGTGAGTTT GACAACTGCG GCTGGTGTCG AGGATGGGAG GCAAGGGGAG 
GGCACGGAGT GGGGTGGAGG CGAGGCGGTG GTGGGCATGG ACATGGAGGA GACAGACGGG 
CAAATGCTCG TGTTCGGCGA CGGTGAGGGC AAGGTTCACG TATGCCGTTT GAAGTGGAAG 
ATGCTGGCCG ACTTGCATGA GCAGACGGCT GCTCTCGCGA TGCCTAATCT GGCCTTGTAC 
TGCCGGACGC CAACGCCAAG GAAGGAGCAA CTTCCCGCGG TTGCAGCGTC GACGGCGAGC 
TCAGCAGAGG ATGCGACGGC TTCCGAGCGG GCGCTGCCGG CGCTGCAGGA ACTGGAGCGC 
GTGCACGTGT TTTCAAGTGG GCTCAAGCTT TCCGGCGTTC GTCTTGTCCA CGCAGACGAG 
GAGACCGCGG TCTTCCGTGC CGCCGTGCCG CTGCCCAAGA CGAAGACTGG CGCTGAAGAT 
GTTGGTACGG CACCGGAAGA GGGCGGGGGC AGAGACGCAC CCAGTTCCTC GCCGGGGACG 
CCCAGGGATA CGGACAGGCC TCGTCTGCTC ATCATCTGCA CTGGCATGGA CCACTTCGTG 
CGTGTCTTCA CGTTGGCAGG CGTCCCTATT GGAGAACTCG GAATGGACGA GTGGGACACG 
GCGCGGCCGT CGACCTTCCG CTTCATGGGC GAGCCGACCG TCCCGCTGCC GTGCTCGCTG 
GCCGGCAACT TCACGTGGCA GCAAGACGGA CACGACAAAA TCAAGAGGAG CGGCTGCTAC 
CACGATTACT TGGCCGACCT GTACGCGACG CACCATGCAC GACCTGCGAT GCTAACCGGG 
CCTACCAAGG GGCTTGCATG CAGTGGCAAC GTGCCGCCGG GCAGCTCCGC AGCGTTACGA 
CGAACAATGG GCGGAACCGT CTACCTTGGC TGTCCCGACA TCCCCCCATC ACGCGCCACC 
GTCAGGGCGT CGTTGGTGGC CCGTCCGTCG CCTGCTCCCG ACGCCCCGCA CCCAGTGGAC 
AGGGTCAGGA GCGCCGCGGG AGTGGACAAG CGGCAGAGCC ACGGGCACTC AAAAGTCGTC 
GGCTTCACGA AGAACGTCGA AACGGGCACG AGGGCTGCGC CCGAGGTGTT CTCCGAGGAC 
GGTCGCAGTC TTCTCCCGCC TCAGCTGAGC CCCGACCAAC GGCGCCCCGC CAGCCAAGGC 
TTCCACGCAC GCCTCCGGCA AAACATGCTG CATTTGTGCG AGAAAGATCG ACCCCTTCAT 
GGGGTGCTAA AGCACTCGTA TGTACGTCAG CTCCAAAACC TCCACCGCCA GGGGCACCCA 
TCGTCGTTGT CGTCGTTCCA ACAGCCACTG GCAGCGCTAC CCGAGCTGCC TTCCATCCTG 
CCCAGTACCT CCGCGACACC ATCTGCTGCA GCCAGCGAGC ACAGCGAATC CGCTGGACAA 
AGCCAGGCTG CAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

The genome of the kinetoplastid parasite, Leishmania major.
Science (New York, N.Y.)309(5733)436-42(2005 Jul)
Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ

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LMJF_33_0950 cDNA ORF clone, Leishmania major strain Friedlin

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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