Espl1 cDNA ORF clone, Cavia porcellus(domestic guinea pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	Espl1
Entrez Gene ID	100733255
Full Name	extra spindle pole bodies like 1, separase
Gene Type	protein-coding
Organism	Cavia porcellus(domestic guinea pig)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013159375.1	XP_013014829.1	separin isoform X3
XM_013159373.1	XP_013014827.1	separin isoform X1
XM_013159373.2	XP_013014827.1	separin isoform X1
XM_013159375.2	XP_013014829.1	separin isoform X3
XM_013159374.1	XP_013014828.1	separin isoform X2
XM_023567606.1	XP_023423374.1	separin isoform X2

Pathways from BioSystems
Gene Ontology

The following Espl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Espl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODo37678	XM_013159373.2 Latest version!	Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODo37679	XM_023567606.1 Latest version!	Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODo37679	XM_013159374.1 Latest version!	Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODo37680	XM_013159375.2 Latest version!	Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
ODo37678	XM_013159373.1	Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODo37680	XM_013159375.1	Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Espl1 ( XM_013159373.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013014827.1 Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X1, mRNA.

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CloneID	ODo37678
Clone ID Related Accession (Same CDS sequence)	XM_013159373.1 , XM_013159373.2
Accession Version	XM_013159373.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176410.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTCC CCTGTGGCTT TTTCTGTGCT ATGAGGAACT TCAAAGGAGT CAACTTTGGA 
ACTCTGCTTA GAAGCCAGAA GGATGCTGAA GAGTTGCTGT CTGACTTGAA GGAGTTCTTG 
TCCAAGCCTC CAGCAGGTTT TCCCAGTAGC CGATCTGACT CTGAGAGGAG ACAAACTTGT 
GATGCCATCC TGAGGGCTTG CAACCAACAG CTGACGGCCA AGCTGTCTTG CCCTCAACAC 
CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG GCCTGTGATG GCTATGTGAT GTCCACTCCC 
CAGCGCCCTC CACTCTACCT GGAACGCATT CTTTTCATTT TACTACGGAA TGTTGCTGCT 
CAGGGAAGCC CCATGGCCAT GCTCCACCTT GCCCAGCCTC TCCATGCCTG CTTCGTTCAG 
TGCTCTCGCC AAGCTGCCTC ACAGGACTAT GAGGCTGTGG CTTGGGGCAG CTTTTCTCTG 
CTTTGGAAGG GAGCAGAAGC ACTGTTGGAG CAGAGAGATG CCTTCTCAGC GCGGATGAAG 
GCCTTGAGCT TCCTTGTCCT CCTGGAGGAT GAAAGTACCC CGTGTGAGAT TCCTCACTTT 
GCTTCTCCAA CAGCCTGTCG AGCAGTAGCT GCCTATCAGC TGTTTGATCG CAGTGGTTGT 
GGTATAAACA AAGCAGATGC TGATTTCTTA TATGATTTGC TCTCCAAAAA TGTGATCAGA 
ACCTTGGTGG GTGAGGAAAG TGGCTCTCCC AGTCCTCTTT CTTCCCAAAG GGCCCTCTGC 
CTCTTGGAGC TCACCTTAGA ACACTGCCGT CGCCTCTGCT TTAGCCACCA CTATGACAAG 
GCCACCAGTG CAGTTGAGAA GGCTCGTGGT TACCTAAGGG ACACCAGTGT AGCCTCCAGC 
CTCCAGCTAT GCCAGTTGGG GGTTGAGCTG CTACATGTTG GGGTGGAAGG ATCCCAGGCA 
GTGGCCCAGC TTCTCATCAA GGCATCAGCT GTCTTGAATA ACAGTTTGAA GGCCCCATCA 
CCTCCACTTC GGGCATTGTT TGAGACCTCC CAGTTCCTCC TTTCAGGCCT GGAACGAGGC 
ATCAAAAGAC ACTCTGGACC TGATGCTATT GGGAGCCTCT TCACTTTTCT TGGAGGATAC 
TGCTTAATTA TCCAGCAGCT ACGGAATGAT GGTGCCTGTG TGCACTCTCC CAAACAGCAG 
CAGTCTTTGG TGCAGACGCA CTTTCAGGGT CTTCACCTCT ACACTATGAT GGTTTATGAC 
TTTGCCCAGC GCTGTCAGGT AGCTGATTTG GCTGACCTGG CTCAACTAGT GGACAGTTGC 
AGATCTACTG CTGTTTGGAT GCTGGAAGCC TTGGAAGGCC TGTCAGGCCT GGAGCTGACT 
GACTACATGG CCATGACTGC CTCTTACACC AGTAACTTGG CCTATAGCTT CTACAATCAC 
AAGCTGTACG CCGAGGCCTG TGCCGTCTCT GAGCCACTCT GTCAGCACCT GGGCTCTGCT 
AAGCCAGGCA CTTACCATGC GGTGCCTTCT GAGAAGTTGC ACAGGTACTT CCGGCTACAC 
GTAGAGAGTT TGAAGAAACT GGGTAAACAG GCACAGGGCT GTGAGATGGT GACCTTGTGG 
CTGGCGGCTC TACAGCCCTG CAACCCTGAA CACATGGCTG AGCCCGTCTC TTTCTGGGTT 
CGGGTAAAGA TGGATGCGAC CAGGGCTGGA AACAAGGAGC TACAGCTGAA GACTCTGCGA 
GACAGCCTGA GTGACTGGAA CCCAGAGACC CTGACCCTCC TGCTAAGGGA GGAGCTGCAG 
GCCTACAAGG CAGTACGGGC TGACACTGGG CAGGAACGTT TCAATGTCAT CTGTGACTTG 
CTGGAGCTGA GCCCTGAGGA GACACCAGCT GGGGCCTGGG CTCGAGCCAC CTACCTGGTG 
GAACTGGCTC AGGTGCTTTG CTACCACGAC TTTGCCCAGC AGACAGACTG CTCTGCCCTG 
GATGCTATCC AGGAAGCCCT GCAGCTTCTG GAGGCTGTTA AGCCAGAGGC CCAGGCCAAG 
GATCGGCTTC TGGACGATAA AGCACAGGCA CTGTTGTGGT TCTACATCTG CACTTTGGAG 
GCCAAAATGC GGGAAGGTAT TGAGCGGGAT CGGAGAGCCC AGGCTCCTAG TAACCTGGAG 
GAATTTGAAG TTAATGATCT CAACTATGAA GATAAACTCC AGGAAGATTG TTTCCTGTAC 
AGTAACATTG CCTTTAACCT GGCTGCAGAT GCAGCTCACT CTAAAGCCCT GGACCAAGCC 
CTGGCTCTGT GGAAGGAGGT CCTTACAGAG GGGCAGTTCC CAGCTGTGCG ATGTCTCCAG 
CAGACAGCAG CCTCACTGCA GATCCTTGTG GCCCTCTATC AGCTGGTGGC CAAGCCTCTG 
CAGGCCCTGG AGGCTCTCCT GCTCCTGCGG ACCATCTCTG AAAGACTGAA GGATCACGCA 
AAGGCAGCTA GCTCCTCTTG CCACCTCACC CAGGTCCTCC TGAGCCTCGG CTGTCCCACC 
TATGCCCAGT TTTACCTGGA AGAGGCTGAA TCCGCCCTGA TGCATCTCAG TGAGACCACG 
GACACACGAC TGCTGCTTTC TCTGACGTGT CACCTCCTTC GAAGTCAGCT CTGCTGGACT 
CAACAGAAGG TGACTGAGGG TGTCTCTCTG TTGCTGACTG TCCTTCGGGA TCCTGCCCTC 
CAGAAGTCAT CCAAGGCTTG GTATTTGCTT CGAGTCCAGG CTCTGCAACT ACTGTCCATC 
TACCTTAACC TCTCATCAAA CAGCCTCTCA GATGCTCTGC GAGAGCAGCT TTGGGCTCAA 
GGCTGGCAGA CACCTGAGAC AGCACTCATA GATTCTCATA AGCTCCTTCG AAGCATCATC 
CTCCTGCTGA TGGGCAGCGA TGTCCTCTCC CTACAGAAAG CAGCTGTGGA GACACCATTT 
CTGGATTGTG GTGAAAATCT GGTACAAAAA TGGCAGGTTC TGTCAGAGGT TCTGAGCTGC 
TCACAGAAGC TGGTCTGCTC TCTTGGCCAC TTAGGGAGTG TGAGTGAAGC CAAGGCCTTT 
TGCTTGGATG CCCTGAAACT GACGACAAAG CTGCAGCTCC CGGGCCAGTG TGCCCTGTTC 
CTGGTGCTGA AAGGTGAGCT GGAACTGGCC CGCAGCGACC TTGACCTCTG TCAGTCGGAC 
CTGCAGCAGG TTCTGTTCCT GCTTGAGTCT TGTACGGAGT TTGCCGGAGT GACCCAGCAC 
CCAGATACTA TAAAGAAGGT CCACCTACAG AAGGGGAAGC AGCAGGCCAG GGTCCCCTGG 
CCCCCGCAGC TCCTGGAGGA GGAGCCTTTC CTAAAAGGTC CTGCTCTGGA GCTGGTGGCC 
ACTGTAGCCA AGGAACCTGG GCCCATTGCA CCTTCGACAA ACTCCTCCCC CATCCTGAAA 
ACCAAGCCCC AACCCCGCCC CGGCTTCCTG TCTCACTCTG CTTCATGTGA CTGCTCGATC 
TGTGCCAGCC CTGTCCTGTC AGCAGTCTGT CTGCGCTGGG TGTTGGTCAC AGCAGGCGTG 
AGGCTGGCCA CAGGCCATCA GGCTCAGGGT CTAGAGCTGC TGCAGGTTGT TCTAAAGGGT 
TGCCCTGCAG CCACCAAGCG CCTCACCCAA ACTCTCCAGG CTTCCCTGAA TCACAGAGCC 
CCTGCCTCTC CTGACCCAAG CCTTCTTGAT GAGCTCCTGG CTCAGGCATA CACACAGCTG 
GCGCTGGCAG GCCTGAAGCA GCCATCAAAC AAGAGCCTGC AGAAGATCTT AGAGTCAGGG 
CTGAAGTTCA CAGCAGCGCG GATCCAACAG CTGGAGCCCT GGCGAGCCAG CCTGCTCTTG 
ATTCAGGCCC TCGTAAAGCT GGCTGGCCTC AGCACCTGTA CTACCCAACT TTTTGCAAGC 
TCCTGGGGCT GGCAGCCACC ATTAATCAAA AGCCCCCCGG GCTCAGAGTC CTCTAAGATC 
CAGAGCCAAA AACATAGTGG ACGAGGCCAC CAGCAAGTAG CCTCTGCTCC CCTGCCCCTC 
CATAATGCCT CTCACAGAAG CCCAGAAGGT GGTGGACCAC CCTGTACACC TAAGCCTGCA 
GGCCGAGTGA GGCCGGCTGG CCCTCGTGTC CCCTTCACTG TGTTTGAAGA TGACTGCCCT 
ACGAAGAACA AGCCCGAGGT TCCCCAGGCC CCCCGGGTAC ATCAGAGGGT CCAGACACGC 
CTTAAGGTGA ACTTCAGCGA TGACAGTGAC TTGGAAGACC CTGTCTCTGC TGAGATACCG 
CTTGTTGAGG AACCTAAGAG ACGGGGCACT GCTTCCTCGG GCCGGGGCCG GGGCCGGGGC 
CGGGCTAGGA AGAGCCCAAG CTTGGCGATA GGTACAGTGG CTGCTCCAGG TAATACCCCA 
ATGAGTGGTA AGAACCGTAG AACCAAGAAG GTGGCATCAA GGCTCTGTGA AGAGCAGGGT 
CCCCAGACAG CCCCTTGTGG CCAGAACAAG CTGGGCCCTG AGCTCATGAG GACTATCCCT 
GAGGAGAAAC TGACTGACAA GTGCACGGAA ATGAGCTTTG AGACCCTTGG AGCCTCTGAC 
AGAGAAGACT CAGCTTCAGG TGGGAAGGCA CTGGTTCCAG GTGCTGATCC CCCCGTAGGT 
GAATGTGAGG TGTTGAGACG GGATTCCAGC AAGGAGGAGC TACCCATCCT GGAACCAGGC 
AAGGAAAGCA ACAGGGACGT CGGTGCTTGG CTCCAACCCC CCGTAACCAT TGGTTCTTCT 
ACCCTGGATT CCATGCGCGA CTCTCTGAGC ATTGCTTTCC GTGGGGTCAG TCATTGCCCT 
GCTAGTGGGC TCTATGCCCA CCTCTGCCGC CTCCTGGCCT TGTGCCTGGG CCACCGGGAT 
CCCTATGCTA CTGCCTTCCT CGTTGCCGAG TCTATCTCTA TTACCTGTCG CCACCAGCTA 
CTCACTCACC TCCACAGACT GCTCAGCAAG GCCCAGAAGC ATCAAGGACC ACTCAGACTG 
ACAGAGCAGC TGCAGCAGCT AACCCTCCAG GAGATGCCTG ATGATGTCCC TCTGGCCCGC 
ATCCAGCGCC TCTTTTCCTT CAGGGCTTCA GGAGCTGGTG CCTTTCCCCA GCCCGAGAAG 
CAGCATTTCC AGGAGCTCTT GGTGCTGCTC CCCAAGGGGG TGACTGTCTG CATGTTGGCT 
GTGTCCACCC TCCAGCCTGG AACTGTGGGC AACACTCTCC TACTGACAAG ACTGGAAAAG 
GACAACCCCC CAGTCACTGT GCAGATCCCC ACTGCCCAGG ATAAGTTCCC TCTGAGTTCA 
GCCCTGAGAG AATTTGACGC TATCCAGAAG GAACAGAAAG AGAACAGCAG CTGCACTGAC 
AAGCGAGCCT GGTGGACAGG CAGACTGGCC CTGGACCACA GAATGGAGGA CCTCATCACT 
TCCCTGGAGA AGCATGTGTT GGGCTGCTGG CGAGGGCTGC TGCTGCCCTC CAGTGAAGAA 
CCTGGCCCTG AGCATGAGGC CACCCGCCTG CAGGAGTTGC TGCAGGACTG TGGCTGGCAG 
TATCCCGACC CGACTCTGTT AAAAGTCTTG CTCAGTGGTG CCAGCACCCT CACAACCCAG 
GATATTCAGG CCCTGGCCTA TGGGCTGTGC CCGACCCAGC CAGCCAGAGC CCAGGAACTC 
CTGACTGAGG CAGCCAGGCG TCTACAGGGG CAGTCAGCAC CAAGCAATAA GCATCTTGTC 
TTGGTGCTGG ACAGGGACCT GCAGAAGCTG CCATGGGAAA GCATACCCTG CCTGCGAGCA 
CTGCCTGTCA CCCGGCTGCC CTCCTTCCAC TTCCTGCTCA GCTATTTCAT CCTCAAAGAG 
GCTGGGGCTG CATCAGTGCT GAGCCAGGGG GTGGATCCTC GGAGCACATT TTACGTCCTG 
AACCCTCACA ATAACCTGGC AAGCACAGAG GAGCAATTTC GAGCCAAGTT TAGCAGTGAA 
GCTGGCTGGA GAGGGGTGGT CGGGGAGGTG CCAAGCCCAG AACAGATGCA GATGGCCTTG 
ACAAAGAACG ACTTATATAT CTATGCAGGT CACGGGGCTG GTGCCCGCTT CCTTGATGGG 
CAGGCAGTAC TGCGGCTAAG CTGCCGGGCA GTGGCCTTGT TGTTTGGCTG CAGCAGCGCA 
GCCCTGGCCG TGCATGGAAA CCTGGAGGGG GCTGGCATTG TGCTCAAGTA CATCATGGCT 
GGCTGCCCCC TGTTTCTGGG AAATCTCTGG GATGTGACTG ACCGTGACAT TGACCGCTAC 
ACAGAAGCTC TCCTGCAGGG ATGGCTTGCA GCAGGCCCAG GGGCCCCTCT GCTCCACTAT 
GTCAGCCAGG CCCGCCAGGC TCCCCGACTC AAGTCTCTTA TTGGAGCTGC ACCTGTTGCC 
TATGGCTTAC CTGTCTTCCT TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013014827.1
CDS	677..7063
Translation	MCFPCGFFCAMRNFKGVNFGTLLRSQKDAEELLSDLKEFLSKPPAGFPSSRSDSERRQTCDAILRACNQQLTAKLSCPQHLGSLLELAELACDGYVMSTPQRPPLYLERILFILLRNVAAQGSPMAMLHLAQPLHACFVQCSRQAASQDYEAVAWGSFSLLWKGAEALLEQRDAFSARMKALSFLVLLEDESTPCEIPHFASPTACRAVAAYQLFDRSGCGINKADADFLYDLLSKNVIRTLVGEESGSPSPLSSQRALCLLELTLEHCRRLCFSHHYDKATSAVEKARGYLRDTSVASSLQLCQLGVELLHVGVEGSQAVAQLLIKASAVLNNSLKAPSPPLRALFETSQFLLSGLERGIKRHSGPDAIGSLFTFLGGYCLIIQQLRNDGACVHSPKQQQSLVQTHFQGLHLYTMMVYDFAQRCQVADLADLAQLVDSCRSTAVWMLEALEGLSGLELTDYMAMTASYTSNLAYSFYNHKLYAEACAVSEPLCQHLGSAKPGTYHAVPSEKLHRYFRLHVESLKKLGKQAQGCEMVTLWLAALQPCNPEHMAEPVSFWVRVKMDATRAGNKELQLKTLRDSLSDWNPETLTLLLREELQAYKAVRADTGQERFNVICDLLELSPEETPAGAWARATYLVELAQVLCYHDFAQQTDCSALDAIQEALQLLEAVKPEAQAKDRLLDDKAQALLWFYICTLEAKMREGIERDRRAQAPSNLEEFEVNDLNYEDKLQEDCFLYSNIAFNLAADAAHSKALDQALALWKEVLTEGQFPAVRCLQQTAASLQILVALYQLVAKPLQALEALLLLRTISERLKDHAKAASSSCHLTQVLLSLGCPTYAQFYLEEAESALMHLSETTDTRLLLSLTCHLLRSQLCWTQQKVTEGVSLLLTVLRDPALQKSSKAWYLLRVQALQLLSIYLNLSSNSLSDALREQLWAQGWQTPETALIDSHKLLRSIILLLMGSDVLSLQKAAVETPFLDCGENLVQKWQVLSEVLSCSQKLVCSLGHLGSVSEAKAFCLDALKLTTKLQLPGQCALFLVLKGELELARSDLDLCQSDLQQVLFLLESCTEFAGVTQHPDTIKKVHLQKGKQQARVPWPPQLLEEEPFLKGPALELVATVAKEPGPIAPSTNSSPILKTKPQPRPGFLSHSASCDCSICASPVLSAVCLRWVLVTAGVRLATGHQAQGLELLQVVLKGCPAATKRLTQTLQASLNHRAPASPDPSLLDELLAQAYTQLALAGLKQPSNKSLQKILESGLKFTAARIQQLEPWRASLLLIQALVKLAGLSTCTTQLFASSWGWQPPLIKSPPGSESSKIQSQKHSGRGHQQVASAPLPLHNASHRSPEGGGPPCTPKPAGRVRPAGPRVPFTVFEDDCPTKNKPEVPQAPRVHQRVQTRLKVNFSDDSDLEDPVSAEIPLVEEPKRRGTASSGRGRGRGRARKSPSLAIGTVAAPGNTPMSGKNRRTKKVASRLCEEQGPQTAPCGQNKLGPELMRTIPEEKLTDKCTEMSFETLGASDREDSASGGKALVPGADPPVGECEVLRRDSSKEELPILEPGKESNRDVGAWLQPPVTIGSSTLDSMRDSLSIAFRGVSHCPASGLYAHLCRLLALCLGHRDPYATAFLVAESISITCRHQLLTHLHRLLSKAQKHQGPLRLTEQLQQLTLQEMPDDVPLARIQRLFSFRASGAGAFPQPEKQHFQELLVLLPKGVTVCMLAVSTLQPGTVGNTLLLTRLEKDNPPVTVQIPTAQDKFPLSSALREFDAIQKEQKENSSCTDKRAWWTGRLALDHRMEDLITSLEKHVLGCWRGLLLPSSEEPGPEHEATRLQELLQDCGWQYPDPTLLKVLLSGASTLTTQDIQALAYGLCPTQPARAQELLTEAARRLQGQSAPSNKHLVLVLDRDLQKLPWESIPCLRALPVTRLPSFHFLLSYFILKEAGAASVLSQGVDPRSTFYVLNPHNNLASTEEQFRAKFSSEAGWRGVVGEVPSPEQMQMALTKNDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLAAGPGAPLLHYVSQARQAPRLKSLIGAAPVAYGLPVFLR

Target ORF information:

RefSeq Version	XM_013159373.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013159373.1

ORF Insert Sequence:

ATGTGCTTCC CCTGTGGCTT TTTCTGTGCT ATGAGGAACT TCAAAGGAGT CAACTTTGGA 
ACTCTGCTTA GAAGCCAGAA GGATGCTGAA GAGTTGCTGT CTGACTTGAA GGAGTTCTTG 
TCCAAGCCTC CAGCAGGTTT TCCCAGTAGC CGATCTGACT CTGAGAGGAG ACAAACTTGT 
GATGCCATCC TGAGGGCTTG CAACCAACAG CTGACGGCCA AGCTGTCTTG CCCTCAACAC 
CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG GCCTGTGATG GCTATGTGAT GTCCACTCCC 
CAGCGCCCTC CACTCTACCT GGAACGCATT CTTTTCATTT TACTACGGAA TGTTGCTGCT 
CAGGGAAGCC CCATGGCCAT GCTCCACCTT GCCCAGCCTC TCCATGCCTG CTTCGTTCAG 
TGCTCTCGCC AAGCTGCCTC ACAGGACTAT GAGGCTGTGG CTTGGGGCAG CTTTTCTCTG 
CTTTGGAAGG GAGCAGAAGC ACTGTTGGAG CAGAGAGATG CCTTCTCAGC GCGGATGAAG 
GCCTTGAGCT TCCTTGTCCT CCTGGAGGAT GAAAGTACCC CGTGTGAGAT TCCTCACTTT 
GCTTCTCCAA CAGCCTGTCG AGCAGTAGCT GCCTATCAGC TGTTTGATCG CAGTGGTTGT 
GGTATAAACA AAGCAGATGC TGATTTCTTA TATGATTTGC TCTCCAAAAA TGTGATCAGA 
ACCTTGGTGG GTGAGGAAAG TGGCTCTCCC AGTCCTCTTT CTTCCCAAAG GGCCCTCTGC 
CTCTTGGAGC TCACCTTAGA ACACTGCCGT CGCCTCTGCT TTAGCCACCA CTATGACAAG 
GCCACCAGTG CAGTTGAGAA GGCTCGTGGT TACCTAAGGG ACACCAGTGT AGCCTCCAGC 
CTCCAGCTAT GCCAGTTGGG GGTTGAGCTG CTACATGTTG GGGTGGAAGG ATCCCAGGCA 
GTGGCCCAGC TTCTCATCAA GGCATCAGCT GTCTTGAATA ACAGTTTGAA GGCCCCATCA 
CCTCCACTTC GGGCATTGTT TGAGACCTCC CAGTTCCTCC TTTCAGGCCT GGAACGAGGC 
ATCAAAAGAC ACTCTGGACC TGATGCTATT GGGAGCCTCT TCACTTTTCT TGGAGGATAC 
TGCTTAATTA TCCAGCAGCT ACGGAATGAT GGTGCCTGTG TGCACTCTCC CAAACAGCAG 
CAGTCTTTGG TGCAGACGCA CTTTCAGGGT CTTCACCTCT ACACTATGAT GGTTTATGAC 
TTTGCCCAGC GCTGTCAGGT AGCTGATTTG GCTGACCTGG CTCAACTAGT GGACAGTTGC 
AGATCTACTG CTGTTTGGAT GCTGGAAGCC TTGGAAGGCC TGTCAGGCCT GGAGCTGACT 
GACTACATGG CCATGACTGC CTCTTACACC AGTAACTTGG CCTATAGCTT CTACAATCAC 
AAGCTGTACG CCGAGGCCTG TGCCGTCTCT GAGCCACTCT GTCAGCACCT GGGCTCTGCT 
AAGCCAGGCA CTTACCATGC GGTGCCTTCT GAGAAGTTGC ACAGGTACTT CCGGCTACAC 
GTAGAGAGTT TGAAGAAACT GGGTAAACAG GCACAGGGCT GTGAGATGGT GACCTTGTGG 
CTGGCGGCTC TACAGCCCTG CAACCCTGAA CACATGGCTG AGCCCGTCTC TTTCTGGGTT 
CGGGTAAAGA TGGATGCGAC CAGGGCTGGA AACAAGGAGC TACAGCTGAA GACTCTGCGA 
GACAGCCTGA GTGACTGGAA CCCAGAGACC CTGACCCTCC TGCTAAGGGA GGAGCTGCAG 
GCCTACAAGG CAGTACGGGC TGACACTGGG CAGGAACGTT TCAATGTCAT CTGTGACTTG 
CTGGAGCTGA GCCCTGAGGA GACACCAGCT GGGGCCTGGG CTCGAGCCAC CTACCTGGTG 
GAACTGGCTC AGGTGCTTTG CTACCACGAC TTTGCCCAGC AGACAGACTG CTCTGCCCTG 
GATGCTATCC AGGAAGCCCT GCAGCTTCTG GAGGCTGTTA AGCCAGAGGC CCAGGCCAAG 
GATCGGCTTC TGGACGATAA AGCACAGGCA CTGTTGTGGT TCTACATCTG CACTTTGGAG 
GCCAAAATGC GGGAAGGTAT TGAGCGGGAT CGGAGAGCCC AGGCTCCTAG TAACCTGGAG 
GAATTTGAAG TTAATGATCT CAACTATGAA GATAAACTCC AGGAAGATTG TTTCCTGTAC 
AGTAACATTG CCTTTAACCT GGCTGCAGAT GCAGCTCACT CTAAAGCCCT GGACCAAGCC 
CTGGCTCTGT GGAAGGAGGT CCTTACAGAG GGGCAGTTCC CAGCTGTGCG ATGTCTCCAG 
CAGACAGCAG CCTCACTGCA GATCCTTGTG GCCCTCTATC AGCTGGTGGC CAAGCCTCTG 
CAGGCCCTGG AGGCTCTCCT GCTCCTGCGG ACCATCTCTG AAAGACTGAA GGATCACGCA 
AAGGCAGCTA GCTCCTCTTG CCACCTCACC CAGGTCCTCC TGAGCCTCGG CTGTCCCACC 
TATGCCCAGT TTTACCTGGA AGAGGCTGAA TCCGCCCTGA TGCATCTCAG TGAGACCACG 
GACACACGAC TGCTGCTTTC TCTGACGTGT CACCTCCTTC GAAGTCAGCT CTGCTGGACT 
CAACAGAAGG TGACTGAGGG TGTCTCTCTG TTGCTGACTG TCCTTCGGGA TCCTGCCCTC 
CAGAAGTCAT CCAAGGCTTG GTATTTGCTT CGAGTCCAGG CTCTGCAACT ACTGTCCATC 
TACCTTAACC TCTCATCAAA CAGCCTCTCA GATGCTCTGC GAGAGCAGCT TTGGGCTCAA 
GGCTGGCAGA CACCTGAGAC AGCACTCATA GATTCTCATA AGCTCCTTCG AAGCATCATC 
CTCCTGCTGA TGGGCAGCGA TGTCCTCTCC CTACAGAAAG CAGCTGTGGA GACACCATTT 
CTGGATTGTG GTGAAAATCT GGTACAAAAA TGGCAGGTTC TGTCAGAGGT TCTGAGCTGC 
TCACAGAAGC TGGTCTGCTC TCTTGGCCAC TTAGGGAGTG TGAGTGAAGC CAAGGCCTTT 
TGCTTGGATG CCCTGAAACT GACGACAAAG CTGCAGCTCC CGGGCCAGTG TGCCCTGTTC 
CTGGTGCTGA AAGGTGAGCT GGAACTGGCC CGCAGCGACC TTGACCTCTG TCAGTCGGAC 
CTGCAGCAGG TTCTGTTCCT GCTTGAGTCT TGTACGGAGT TTGCCGGAGT GACCCAGCAC 
CCAGATACTA TAAAGAAGGT CCACCTACAG AAGGGGAAGC AGCAGGCCAG GGTCCCCTGG 
CCCCCGCAGC TCCTGGAGGA GGAGCCTTTC CTAAAAGGTC CTGCTCTGGA GCTGGTGGCC 
ACTGTAGCCA AGGAACCTGG GCCCATTGCA CCTTCGACAA ACTCCTCCCC CATCCTGAAA 
ACCAAGCCCC AACCCCGCCC CGGCTTCCTG TCTCACTCTG CTTCATGTGA CTGCTCGATC 
TGTGCCAGCC CTGTCCTGTC AGCAGTCTGT CTGCGCTGGG TGTTGGTCAC AGCAGGCGTG 
AGGCTGGCCA CAGGCCATCA GGCTCAGGGT CTAGAGCTGC TGCAGGTTGT TCTAAAGGGT 
TGCCCTGCAG CCACCAAGCG CCTCACCCAA ACTCTCCAGG CTTCCCTGAA TCACAGAGCC 
CCTGCCTCTC CTGACCCAAG CCTTCTTGAT GAGCTCCTGG CTCAGGCATA CACACAGCTG 
GCGCTGGCAG GCCTGAAGCA GCCATCAAAC AAGAGCCTGC AGAAGATCTT AGAGTCAGGG 
CTGAAGTTCA CAGCAGCGCG GATCCAACAG CTGGAGCCCT GGCGAGCCAG CCTGCTCTTG 
ATTCAGGCCC TCGTAAAGCT GGCTGGCCTC AGCACCTGTA CTACCCAACT TTTTGCAAGC 
TCCTGGGGCT GGCAGCCACC ATTAATCAAA AGCCCCCCGG GCTCAGAGTC CTCTAAGATC 
CAGAGCCAAA AACATAGTGG ACGAGGCCAC CAGCAAGTAG CCTCTGCTCC CCTGCCCCTC 
CATAATGCCT CTCACAGAAG CCCAGAAGGT GGTGGACCAC CCTGTACACC TAAGCCTGCA 
GGCCGAGTGA GGCCGGCTGG CCCTCGTGTC CCCTTCACTG TGTTTGAAGA TGACTGCCCT 
ACGAAGAACA AGCCCGAGGT TCCCCAGGCC CCCCGGGTAC ATCAGAGGGT CCAGACACGC 
CTTAAGGTGA ACTTCAGCGA TGACAGTGAC TTGGAAGACC CTGTCTCTGC TGAGATACCG 
CTTGTTGAGG AACCTAAGAG ACGGGGCACT GCTTCCTCGG GCCGGGGCCG GGGCCGGGGC 
CGGGCTAGGA AGAGCCCAAG CTTGGCGATA GGTACAGTGG CTGCTCCAGG TAATACCCCA 
ATGAGTGGTA AGAACCGTAG AACCAAGAAG GTGGCATCAA GGCTCTGTGA AGAGCAGGGT 
CCCCAGACAG CCCCTTGTGG CCAGAACAAG CTGGGCCCTG AGCTCATGAG GACTATCCCT 
GAGGAGAAAC TGACTGACAA GTGCACGGAA ATGAGCTTTG AGACCCTTGG AGCCTCTGAC 
AGAGAAGACT CAGCTTCAGG TGGGAAGGCA CTGGTTCCAG GTGCTGATCC CCCCGTAGGT 
GAATGTGAGG TGTTGAGACG GGATTCCAGC AAGGAGGAGC TACCCATCCT GGAACCAGGC 
AAGGAAAGCA ACAGGGACGT CGGTGCTTGG CTCCAACCCC CCGTAACCAT TGGTTCTTCT 
ACCCTGGATT CCATGCGCGA CTCTCTGAGC ATTGCTTTCC GTGGGGTCAG TCATTGCCCT 
GCTAGTGGGC TCTATGCCCA CCTCTGCCGC CTCCTGGCCT TGTGCCTGGG CCACCGGGAT 
CCCTATGCTA CTGCCTTCCT CGTTGCCGAG TCTATCTCTA TTACCTGTCG CCACCAGCTA 
CTCACTCACC TCCACAGACT GCTCAGCAAG GCCCAGAAGC ATCAAGGACC ACTCAGACTG 
ACAGAGCAGC TGCAGCAGCT AACCCTCCAG GAGATGCCTG ATGATGTCCC TCTGGCCCGC 
ATCCAGCGCC TCTTTTCCTT CAGGGCTTCA GGAGCTGGTG CCTTTCCCCA GCCCGAGAAG 
CAGCATTTCC AGGAGCTCTT GGTGCTGCTC CCCAAGGGGG TGACTGTCTG CATGTTGGCT 
GTGTCCACCC TCCAGCCTGG AACTGTGGGC AACACTCTCC TACTGACAAG ACTGGAAAAG 
GACAACCCCC CAGTCACTGT GCAGATCCCC ACTGCCCAGG ATAAGTTCCC TCTGAGTTCA 
GCCCTGAGAG AATTTGACGC TATCCAGAAG GAACAGAAAG AGAACAGCAG CTGCACTGAC 
AAGCGAGCCT GGTGGACAGG CAGACTGGCC CTGGACCACA GAATGGAGGA CCTCATCACT 
TCCCTGGAGA AGCATGTGTT GGGCTGCTGG CGAGGGCTGC TGCTGCCCTC CAGTGAAGAA 
CCTGGCCCTG AGCATGAGGC CACCCGCCTG CAGGAGTTGC TGCAGGACTG TGGCTGGCAG 
TATCCCGACC CGACTCTGTT AAAAGTCTTG CTCAGTGGTG CCAGCACCCT CACAACCCAG 
GATATTCAGG CCCTGGCCTA TGGGCTGTGC CCGACCCAGC CAGCCAGAGC CCAGGAACTC 
CTGACTGAGG CAGCCAGGCG TCTACAGGGG CAGTCAGCAC CAAGCAATAA GCATCTTGTC 
TTGGTGCTGG ACAGGGACCT GCAGAAGCTG CCATGGGAAA GCATACCCTG CCTGCGAGCA 
CTGCCTGTCA CCCGGCTGCC CTCCTTCCAC TTCCTGCTCA GCTATTTCAT CCTCAAAGAG 
GCTGGGGCTG CATCAGTGCT GAGCCAGGGG GTGGATCCTC GGAGCACATT TTACGTCCTG 
AACCCTCACA ATAACCTGGC AAGCACAGAG GAGCAATTTC GAGCCAAGTT TAGCAGTGAA 
GCTGGCTGGA GAGGGGTGGT CGGGGAGGTG CCAAGCCCAG AACAGATGCA GATGGCCTTG 
ACAAAGAACG ACTTATATAT CTATGCAGGT CACGGGGCTG GTGCCCGCTT CCTTGATGGG 
CAGGCAGTAC TGCGGCTAAG CTGCCGGGCA GTGGCCTTGT TGTTTGGCTG CAGCAGCGCA 
GCCCTGGCCG TGCATGGAAA CCTGGAGGGG GCTGGCATTG TGCTCAAGTA CATCATGGCT 
GGCTGCCCCC TGTTTCTGGG AAATCTCTGG GATGTGACTG ACCGTGACAT TGACCGCTAC 
ACAGAAGCTC TCCTGCAGGG ATGGCTTGCA GCAGGCCCAG GGGCCCCTCT GCTCCACTAT 
GTCAGCCAGG CCCGCCAGGC TCCCCGACTC AAGTCTCTTA TTGGAGCTGC ACCTGTTGCC 
TATGGCTTAC CTGTCTTCCT TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espl1 ( XM_013159373.2 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013014827.1 Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X1, mRNA.

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CloneID	ODo37678
Clone ID Related Accession (Same CDS sequence)	XM_013159373.1 , XM_013159373.2
Accession Version	XM_013159373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176410.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_013159373.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTCC CCTGTGGCTT TTTCTGTGCT ATGAGGAACT TCAAAGGAGT CAACTTTGGA 
ACTCTGCTTA GAAGCCAGAA GGATGCTGAA GAGTTGCTGT CTGACTTGAA GGAGTTCTTG 
TCCAAGCCTC CAGCAGGTTT TCCCAGTAGC CGATCTGACT CTGAGAGGAG ACAAACTTGT 
GATGCCATCC TGAGGGCTTG CAACCAACAG CTGACGGCCA AGCTGTCTTG CCCTCAACAC 
CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG GCCTGTGATG GCTATGTGAT GTCCACTCCC 
CAGCGCCCTC CACTCTACCT GGAACGCATT CTTTTCATTT TACTACGGAA TGTTGCTGCT 
CAGGGAAGCC CCATGGCCAT GCTCCACCTT GCCCAGCCTC TCCATGCCTG CTTCGTTCAG 
TGCTCTCGCC AAGCTGCCTC ACAGGACTAT GAGGCTGTGG CTTGGGGCAG CTTTTCTCTG 
CTTTGGAAGG GAGCAGAAGC ACTGTTGGAG CAGAGAGATG CCTTCTCAGC GCGGATGAAG 
GCCTTGAGCT TCCTTGTCCT CCTGGAGGAT GAAAGTACCC CGTGTGAGAT TCCTCACTTT 
GCTTCTCCAA CAGCCTGTCG AGCAGTAGCT GCCTATCAGC TGTTTGATCG CAGTGGTTGT 
GGTATAAACA AAGCAGATGC TGATTTCTTA TATGATTTGC TCTCCAAAAA TGTGATCAGA 
ACCTTGGTGG GTGAGGAAAG TGGCTCTCCC AGTCCTCTTT CTTCCCAAAG GGCCCTCTGC 
CTCTTGGAGC TCACCTTAGA ACACTGCCGT CGCCTCTGCT TTAGCCACCA CTATGACAAG 
GCCACCAGTG CAGTTGAGAA GGCTCGTGGT TACCTAAGGG ACACCAGTGT AGCCTCCAGC 
CTCCAGCTAT GCCAGTTGGG GGTTGAGCTG CTACATGTTG GGGTGGAAGG ATCCCAGGCA 
GTGGCCCAGC TTCTCATCAA GGCATCAGCT GTCTTGAATA ACAGTTTGAA GGCCCCATCA 
CCTCCACTTC GGGCATTGTT TGAGACCTCC CAGTTCCTCC TTTCAGGCCT GGAACGAGGC 
ATCAAAAGAC ACTCTGGACC TGATGCTATT GGGAGCCTCT TCACTTTTCT TGGAGGATAC 
TGCTTAATTA TCCAGCAGCT ACGGAATGAT GGTGCCTGTG TGCACTCTCC CAAACAGCAG 
CAGTCTTTGG TGCAGACGCA CTTTCAGGGT CTTCACCTCT ACACTATGAT GGTTTATGAC 
TTTGCCCAGC GCTGTCAGGT AGCTGATTTG GCTGACCTGG CTCAACTAGT GGACAGTTGC 
AGATCTACTG CTGTTTGGAT GCTGGAAGCC TTGGAAGGCC TGTCAGGCCT GGAGCTGACT 
GACTACATGG CCATGACTGC CTCTTACACC AGTAACTTGG CCTATAGCTT CTACAATCAC 
AAGCTGTACG CCGAGGCCTG TGCCGTCTCT GAGCCACTCT GTCAGCACCT GGGCTCTGCT 
AAGCCAGGCA CTTACCATGC GGTGCCTTCT GAGAAGTTGC ACAGGTACTT CCGGCTACAC 
GTAGAGAGTT TGAAGAAACT GGGTAAACAG GCACAGGGCT GTGAGATGGT GACCTTGTGG 
CTGGCGGCTC TACAGCCCTG CAACCCTGAA CACATGGCTG AGCCCGTCTC TTTCTGGGTT 
CGGGTAAAGA TGGATGCGAC CAGGGCTGGA AACAAGGAGC TACAGCTGAA GACTCTGCGA 
GACAGCCTGA GTGACTGGAA CCCAGAGACC CTGACCCTCC TGCTAAGGGA GGAGCTGCAG 
GCCTACAAGG CAGTACGGGC TGACACTGGG CAGGAACGTT TCAATGTCAT CTGTGACTTG 
CTGGAGCTGA GCCCTGAGGA GACACCAGCT GGGGCCTGGG CTCGAGCCAC CTACCTGGTG 
GAACTGGCTC AGGTGCTTTG CTACCACGAC TTTGCCCAGC AGACAGACTG CTCTGCCCTG 
GATGCTATCC AGGAAGCCCT GCAGCTTCTG GAGGCTGTTA AGCCAGAGGC CCAGGCCAAG 
GATCGGCTTC TGGACGATAA AGCACAGGCA CTGTTGTGGT TCTACATCTG CACTTTGGAG 
GCCAAAATGC GGGAAGGTAT TGAGCGGGAT CGGAGAGCCC AGGCTCCTAG TAACCTGGAG 
GAATTTGAAG TTAATGATCT CAACTATGAA GATAAACTCC AGGAAGATTG TTTCCTGTAC 
AGTAACATTG CCTTTAACCT GGCTGCAGAT GCAGCTCACT CTAAAGCCCT GGACCAAGCC 
CTGGCTCTGT GGAAGGAGGT CCTTACAGAG GGGCAGTTCC CAGCTGTGCG ATGTCTCCAG 
CAGACAGCAG CCTCACTGCA GATCCTTGTG GCCCTCTATC AGCTGGTGGC CAAGCCTCTG 
CAGGCCCTGG AGGCTCTCCT GCTCCTGCGG ACCATCTCTG AAAGACTGAA GGATCACGCA 
AAGGCAGCTA GCTCCTCTTG CCACCTCACC CAGGTCCTCC TGAGCCTCGG CTGTCCCACC 
TATGCCCAGT TTTACCTGGA AGAGGCTGAA TCCGCCCTGA TGCATCTCAG TGAGACCACG 
GACACACGAC TGCTGCTTTC TCTGACGTGT CACCTCCTTC GAAGTCAGCT CTGCTGGACT 
CAACAGAAGG TGACTGAGGG TGTCTCTCTG TTGCTGACTG TCCTTCGGGA TCCTGCCCTC 
CAGAAGTCAT CCAAGGCTTG GTATTTGCTT CGAGTCCAGG CTCTGCAACT ACTGTCCATC 
TACCTTAACC TCTCATCAAA CAGCCTCTCA GATGCTCTGC GAGAGCAGCT TTGGGCTCAA 
GGCTGGCAGA CACCTGAGAC AGCACTCATA GATTCTCATA AGCTCCTTCG AAGCATCATC 
CTCCTGCTGA TGGGCAGCGA TGTCCTCTCC CTACAGAAAG CAGCTGTGGA GACACCATTT 
CTGGATTGTG GTGAAAATCT GGTACAAAAA TGGCAGGTTC TGTCAGAGGT TCTGAGCTGC 
TCACAGAAGC TGGTCTGCTC TCTTGGCCAC TTAGGGAGTG TGAGTGAAGC CAAGGCCTTT 
TGCTTGGATG CCCTGAAACT GACGACAAAG CTGCAGCTCC CGGGCCAGTG TGCCCTGTTC 
CTGGTGCTGA AAGGTGAGCT GGAACTGGCC CGCAGCGACC TTGACCTCTG TCAGTCGGAC 
CTGCAGCAGG TTCTGTTCCT GCTTGAGTCT TGTACGGAGT TTGCCGGAGT GACCCAGCAC 
CCAGATACTA TAAAGAAGGT CCACCTACAG AAGGGGAAGC AGCAGGCCAG GGTCCCCTGG 
CCCCCGCAGC TCCTGGAGGA GGAGCCTTTC CTAAAAGGTC CTGCTCTGGA GCTGGTGGCC 
ACTGTAGCCA AGGAACCTGG GCCCATTGCA CCTTCGACAA ACTCCTCCCC CATCCTGAAA 
ACCAAGCCCC AACCCCGCCC CGGCTTCCTG TCTCACTCTG CTTCATGTGA CTGCTCGATC 
TGTGCCAGCC CTGTCCTGTC AGCAGTCTGT CTGCGCTGGG TGTTGGTCAC AGCAGGCGTG 
AGGCTGGCCA CAGGCCATCA GGCTCAGGGT CTAGAGCTGC TGCAGGTTGT TCTAAAGGGT 
TGCCCTGCAG CCACCAAGCG CCTCACCCAA ACTCTCCAGG CTTCCCTGAA TCACAGAGCC 
CCTGCCTCTC CTGACCCAAG CCTTCTTGAT GAGCTCCTGG CTCAGGCATA CACACAGCTG 
GCGCTGGCAG GCCTGAAGCA GCCATCAAAC AAGAGCCTGC AGAAGATCTT AGAGTCAGGG 
CTGAAGTTCA CAGCAGCGCG GATCCAACAG CTGGAGCCCT GGCGAGCCAG CCTGCTCTTG 
ATTCAGGCCC TCGTAAAGCT GGCTGGCCTC AGCACCTGTA CTACCCAACT TTTTGCAAGC 
TCCTGGGGCT GGCAGCCACC ATTAATCAAA AGCCCCCCGG GCTCAGAGTC CTCTAAGATC 
CAGAGCCAAA AACATAGTGG ACGAGGCCAC CAGCAAGTAG CCTCTGCTCC CCTGCCCCTC 
CATAATGCCT CTCACAGAAG CCCAGAAGGT GGTGGACCAC CCTGTACACC TAAGCCTGCA 
GGCCGAGTGA GGCCGGCTGG CCCTCGTGTC CCCTTCACTG TGTTTGAAGA TGACTGCCCT 
ACGAAGAACA AGCCCGAGGT TCCCCAGGCC CCCCGGGTAC ATCAGAGGGT CCAGACACGC 
CTTAAGGTGA ACTTCAGCGA TGACAGTGAC TTGGAAGACC CTGTCTCTGC TGAGATACCG 
CTTGTTGAGG AACCTAAGAG ACGGGGCACT GCTTCCTCGG GCCGGGGCCG GGGCCGGGGC 
CGGGCTAGGA AGAGCCCAAG CTTGGCGATA GGTACAGTGG CTGCTCCAGG TAATACCCCA 
ATGAGTGGTA AGAACCGTAG AACCAAGAAG GTGGCATCAA GGCTCTGTGA AGAGCAGGGT 
CCCCAGACAG CCCCTTGTGG CCAGAACAAG CTGGGCCCTG AGCTCATGAG GACTATCCCT 
GAGGAGAAAC TGACTGACAA GTGCACGGAA ATGAGCTTTG AGACCCTTGG AGCCTCTGAC 
AGAGAAGACT CAGCTTCAGG TGGGAAGGCA CTGGTTCCAG GTGCTGATCC CCCCGTAGGT 
GAATGTGAGG TGTTGAGACG GGATTCCAGC AAGGAGGAGC TACCCATCCT GGAACCAGGC 
AAGGAAAGCA ACAGGGACGT CGGTGCTTGG CTCCAACCCC CCGTAACCAT TGGTTCTTCT 
ACCCTGGATT CCATGCGCGA CTCTCTGAGC ATTGCTTTCC GTGGGGTCAG TCATTGCCCT 
GCTAGTGGGC TCTATGCCCA CCTCTGCCGC CTCCTGGCCT TGTGCCTGGG CCACCGGGAT 
CCCTATGCTA CTGCCTTCCT CGTTGCCGAG TCTATCTCTA TTACCTGTCG CCACCAGCTA 
CTCACTCACC TCCACAGACT GCTCAGCAAG GCCCAGAAGC ATCAAGGACC ACTCAGACTG 
ACAGAGCAGC TGCAGCAGCT AACCCTCCAG GAGATGCCTG ATGATGTCCC TCTGGCCCGC 
ATCCAGCGCC TCTTTTCCTT CAGGGCTTCA GGAGCTGGTG CCTTTCCCCA GCCCGAGAAG 
CAGCATTTCC AGGAGCTCTT GGTGCTGCTC CCCAAGGGGG TGACTGTCTG CATGTTGGCT 
GTGTCCACCC TCCAGCCTGG AACTGTGGGC AACACTCTCC TACTGACAAG ACTGGAAAAG 
GACAACCCCC CAGTCACTGT GCAGATCCCC ACTGCCCAGG ATAAGTTCCC TCTGAGTTCA 
GCCCTGAGAG AATTTGACGC TATCCAGAAG GAACAGAAAG AGAACAGCAG CTGCACTGAC 
AAGCGAGCCT GGTGGACAGG CAGACTGGCC CTGGACCACA GAATGGAGGA CCTCATCACT 
TCCCTGGAGA AGCATGTGTT GGGCTGCTGG CGAGGGCTGC TGCTGCCCTC CAGTGAAGAA 
CCTGGCCCTG AGCATGAGGC CACCCGCCTG CAGGAGTTGC TGCAGGACTG TGGCTGGCAG 
TATCCCGACC CGACTCTGTT AAAAGTCTTG CTCAGTGGTG CCAGCACCCT CACAACCCAG 
GATATTCAGG CCCTGGCCTA TGGGCTGTGC CCGACCCAGC CAGCCAGAGC CCAGGAACTC 
CTGACTGAGG CAGCCAGGCG TCTACAGGGG CAGTCAGCAC CAAGCAATAA GCATCTTGTC 
TTGGTGCTGG ACAGGGACCT GCAGAAGCTG CCATGGGAAA GCATACCCTG CCTGCGAGCA 
CTGCCTGTCA CCCGGCTGCC CTCCTTCCAC TTCCTGCTCA GCTATTTCAT CCTCAAAGAG 
GCTGGGGCTG CATCAGTGCT GAGCCAGGGG GTGGATCCTC GGAGCACATT TTACGTCCTG 
AACCCTCACA ATAACCTGGC AAGCACAGAG GAGCAATTTC GAGCCAAGTT TAGCAGTGAA 
GCTGGCTGGA GAGGGGTGGT CGGGGAGGTG CCAAGCCCAG AACAGATGCA GATGGCCTTG 
ACAAAGAACG ACTTATATAT CTATGCAGGT CACGGGGCTG GTGCCCGCTT CCTTGATGGG 
CAGGCAGTAC TGCGGCTAAG CTGCCGGGCA GTGGCCTTGT TGTTTGGCTG CAGCAGCGCA 
GCCCTGGCCG TGCATGGAAA CCTGGAGGGG GCTGGCATTG TGCTCAAGTA CATCATGGCT 
GGCTGCCCCC TGTTTCTGGG AAATCTCTGG GATGTGACTG ACCGTGACAT TGACCGCTAC 
ACAGAAGCTC TCCTGCAGGG ATGGCTTGCA GCAGGCCCAG GGGCCCCTCT GCTCCACTAT 
GTCAGCCAGG CCCGCCAGGC TCCCCGACTC AAGTCTCTTA TTGGAGCTGC ACCTGTTGCC 
TATGGCTTAC CTGTCTTCCT TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013014827.1
CDS	679..7065
Translation	MCFPCGFFCAMRNFKGVNFGTLLRSQKDAEELLSDLKEFLSKPPAGFPSSRSDSERRQTCDAILRACNQQLTAKLSCPQHLGSLLELAELACDGYVMSTPQRPPLYLERILFILLRNVAAQGSPMAMLHLAQPLHACFVQCSRQAASQDYEAVAWGSFSLLWKGAEALLEQRDAFSARMKALSFLVLLEDESTPCEIPHFASPTACRAVAAYQLFDRSGCGINKADADFLYDLLSKNVIRTLVGEESGSPSPLSSQRALCLLELTLEHCRRLCFSHHYDKATSAVEKARGYLRDTSVASSLQLCQLGVELLHVGVEGSQAVAQLLIKASAVLNNSLKAPSPPLRALFETSQFLLSGLERGIKRHSGPDAIGSLFTFLGGYCLIIQQLRNDGACVHSPKQQQSLVQTHFQGLHLYTMMVYDFAQRCQVADLADLAQLVDSCRSTAVWMLEALEGLSGLELTDYMAMTASYTSNLAYSFYNHKLYAEACAVSEPLCQHLGSAKPGTYHAVPSEKLHRYFRLHVESLKKLGKQAQGCEMVTLWLAALQPCNPEHMAEPVSFWVRVKMDATRAGNKELQLKTLRDSLSDWNPETLTLLLREELQAYKAVRADTGQERFNVICDLLELSPEETPAGAWARATYLVELAQVLCYHDFAQQTDCSALDAIQEALQLLEAVKPEAQAKDRLLDDKAQALLWFYICTLEAKMREGIERDRRAQAPSNLEEFEVNDLNYEDKLQEDCFLYSNIAFNLAADAAHSKALDQALALWKEVLTEGQFPAVRCLQQTAASLQILVALYQLVAKPLQALEALLLLRTISERLKDHAKAASSSCHLTQVLLSLGCPTYAQFYLEEAESALMHLSETTDTRLLLSLTCHLLRSQLCWTQQKVTEGVSLLLTVLRDPALQKSSKAWYLLRVQALQLLSIYLNLSSNSLSDALREQLWAQGWQTPETALIDSHKLLRSIILLLMGSDVLSLQKAAVETPFLDCGENLVQKWQVLSEVLSCSQKLVCSLGHLGSVSEAKAFCLDALKLTTKLQLPGQCALFLVLKGELELARSDLDLCQSDLQQVLFLLESCTEFAGVTQHPDTIKKVHLQKGKQQARVPWPPQLLEEEPFLKGPALELVATVAKEPGPIAPSTNSSPILKTKPQPRPGFLSHSASCDCSICASPVLSAVCLRWVLVTAGVRLATGHQAQGLELLQVVLKGCPAATKRLTQTLQASLNHRAPASPDPSLLDELLAQAYTQLALAGLKQPSNKSLQKILESGLKFTAARIQQLEPWRASLLLIQALVKLAGLSTCTTQLFASSWGWQPPLIKSPPGSESSKIQSQKHSGRGHQQVASAPLPLHNASHRSPEGGGPPCTPKPAGRVRPAGPRVPFTVFEDDCPTKNKPEVPQAPRVHQRVQTRLKVNFSDDSDLEDPVSAEIPLVEEPKRRGTASSGRGRGRGRARKSPSLAIGTVAAPGNTPMSGKNRRTKKVASRLCEEQGPQTAPCGQNKLGPELMRTIPEEKLTDKCTEMSFETLGASDREDSASGGKALVPGADPPVGECEVLRRDSSKEELPILEPGKESNRDVGAWLQPPVTIGSSTLDSMRDSLSIAFRGVSHCPASGLYAHLCRLLALCLGHRDPYATAFLVAESISITCRHQLLTHLHRLLSKAQKHQGPLRLTEQLQQLTLQEMPDDVPLARIQRLFSFRASGAGAFPQPEKQHFQELLVLLPKGVTVCMLAVSTLQPGTVGNTLLLTRLEKDNPPVTVQIPTAQDKFPLSSALREFDAIQKEQKENSSCTDKRAWWTGRLALDHRMEDLITSLEKHVLGCWRGLLLPSSEEPGPEHEATRLQELLQDCGWQYPDPTLLKVLLSGASTLTTQDIQALAYGLCPTQPARAQELLTEAARRLQGQSAPSNKHLVLVLDRDLQKLPWESIPCLRALPVTRLPSFHFLLSYFILKEAGAASVLSQGVDPRSTFYVLNPHNNLASTEEQFRAKFSSEAGWRGVVGEVPSPEQMQMALTKNDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLAAGPGAPLLHYVSQARQAPRLKSLIGAAPVAYGLPVFLR

Target ORF information:

RefSeq Version	XM_013159373.2
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013159373.2

ORF Insert Sequence:

ATGTGCTTCC CCTGTGGCTT TTTCTGTGCT ATGAGGAACT TCAAAGGAGT CAACTTTGGA 
ACTCTGCTTA GAAGCCAGAA GGATGCTGAA GAGTTGCTGT CTGACTTGAA GGAGTTCTTG 
TCCAAGCCTC CAGCAGGTTT TCCCAGTAGC CGATCTGACT CTGAGAGGAG ACAAACTTGT 
GATGCCATCC TGAGGGCTTG CAACCAACAG CTGACGGCCA AGCTGTCTTG CCCTCAACAC 
CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG GCCTGTGATG GCTATGTGAT GTCCACTCCC 
CAGCGCCCTC CACTCTACCT GGAACGCATT CTTTTCATTT TACTACGGAA TGTTGCTGCT 
CAGGGAAGCC CCATGGCCAT GCTCCACCTT GCCCAGCCTC TCCATGCCTG CTTCGTTCAG 
TGCTCTCGCC AAGCTGCCTC ACAGGACTAT GAGGCTGTGG CTTGGGGCAG CTTTTCTCTG 
CTTTGGAAGG GAGCAGAAGC ACTGTTGGAG CAGAGAGATG CCTTCTCAGC GCGGATGAAG 
GCCTTGAGCT TCCTTGTCCT CCTGGAGGAT GAAAGTACCC CGTGTGAGAT TCCTCACTTT 
GCTTCTCCAA CAGCCTGTCG AGCAGTAGCT GCCTATCAGC TGTTTGATCG CAGTGGTTGT 
GGTATAAACA AAGCAGATGC TGATTTCTTA TATGATTTGC TCTCCAAAAA TGTGATCAGA 
ACCTTGGTGG GTGAGGAAAG TGGCTCTCCC AGTCCTCTTT CTTCCCAAAG GGCCCTCTGC 
CTCTTGGAGC TCACCTTAGA ACACTGCCGT CGCCTCTGCT TTAGCCACCA CTATGACAAG 
GCCACCAGTG CAGTTGAGAA GGCTCGTGGT TACCTAAGGG ACACCAGTGT AGCCTCCAGC 
CTCCAGCTAT GCCAGTTGGG GGTTGAGCTG CTACATGTTG GGGTGGAAGG ATCCCAGGCA 
GTGGCCCAGC TTCTCATCAA GGCATCAGCT GTCTTGAATA ACAGTTTGAA GGCCCCATCA 
CCTCCACTTC GGGCATTGTT TGAGACCTCC CAGTTCCTCC TTTCAGGCCT GGAACGAGGC 
ATCAAAAGAC ACTCTGGACC TGATGCTATT GGGAGCCTCT TCACTTTTCT TGGAGGATAC 
TGCTTAATTA TCCAGCAGCT ACGGAATGAT GGTGCCTGTG TGCACTCTCC CAAACAGCAG 
CAGTCTTTGG TGCAGACGCA CTTTCAGGGT CTTCACCTCT ACACTATGAT GGTTTATGAC 
TTTGCCCAGC GCTGTCAGGT AGCTGATTTG GCTGACCTGG CTCAACTAGT GGACAGTTGC 
AGATCTACTG CTGTTTGGAT GCTGGAAGCC TTGGAAGGCC TGTCAGGCCT GGAGCTGACT 
GACTACATGG CCATGACTGC CTCTTACACC AGTAACTTGG CCTATAGCTT CTACAATCAC 
AAGCTGTACG CCGAGGCCTG TGCCGTCTCT GAGCCACTCT GTCAGCACCT GGGCTCTGCT 
AAGCCAGGCA CTTACCATGC GGTGCCTTCT GAGAAGTTGC ACAGGTACTT CCGGCTACAC 
GTAGAGAGTT TGAAGAAACT GGGTAAACAG GCACAGGGCT GTGAGATGGT GACCTTGTGG 
CTGGCGGCTC TACAGCCCTG CAACCCTGAA CACATGGCTG AGCCCGTCTC TTTCTGGGTT 
CGGGTAAAGA TGGATGCGAC CAGGGCTGGA AACAAGGAGC TACAGCTGAA GACTCTGCGA 
GACAGCCTGA GTGACTGGAA CCCAGAGACC CTGACCCTCC TGCTAAGGGA GGAGCTGCAG 
GCCTACAAGG CAGTACGGGC TGACACTGGG CAGGAACGTT TCAATGTCAT CTGTGACTTG 
CTGGAGCTGA GCCCTGAGGA GACACCAGCT GGGGCCTGGG CTCGAGCCAC CTACCTGGTG 
GAACTGGCTC AGGTGCTTTG CTACCACGAC TTTGCCCAGC AGACAGACTG CTCTGCCCTG 
GATGCTATCC AGGAAGCCCT GCAGCTTCTG GAGGCTGTTA AGCCAGAGGC CCAGGCCAAG 
GATCGGCTTC TGGACGATAA AGCACAGGCA CTGTTGTGGT TCTACATCTG CACTTTGGAG 
GCCAAAATGC GGGAAGGTAT TGAGCGGGAT CGGAGAGCCC AGGCTCCTAG TAACCTGGAG 
GAATTTGAAG TTAATGATCT CAACTATGAA GATAAACTCC AGGAAGATTG TTTCCTGTAC 
AGTAACATTG CCTTTAACCT GGCTGCAGAT GCAGCTCACT CTAAAGCCCT GGACCAAGCC 
CTGGCTCTGT GGAAGGAGGT CCTTACAGAG GGGCAGTTCC CAGCTGTGCG ATGTCTCCAG 
CAGACAGCAG CCTCACTGCA GATCCTTGTG GCCCTCTATC AGCTGGTGGC CAAGCCTCTG 
CAGGCCCTGG AGGCTCTCCT GCTCCTGCGG ACCATCTCTG AAAGACTGAA GGATCACGCA 
AAGGCAGCTA GCTCCTCTTG CCACCTCACC CAGGTCCTCC TGAGCCTCGG CTGTCCCACC 
TATGCCCAGT TTTACCTGGA AGAGGCTGAA TCCGCCCTGA TGCATCTCAG TGAGACCACG 
GACACACGAC TGCTGCTTTC TCTGACGTGT CACCTCCTTC GAAGTCAGCT CTGCTGGACT 
CAACAGAAGG TGACTGAGGG TGTCTCTCTG TTGCTGACTG TCCTTCGGGA TCCTGCCCTC 
CAGAAGTCAT CCAAGGCTTG GTATTTGCTT CGAGTCCAGG CTCTGCAACT ACTGTCCATC 
TACCTTAACC TCTCATCAAA CAGCCTCTCA GATGCTCTGC GAGAGCAGCT TTGGGCTCAA 
GGCTGGCAGA CACCTGAGAC AGCACTCATA GATTCTCATA AGCTCCTTCG AAGCATCATC 
CTCCTGCTGA TGGGCAGCGA TGTCCTCTCC CTACAGAAAG CAGCTGTGGA GACACCATTT 
CTGGATTGTG GTGAAAATCT GGTACAAAAA TGGCAGGTTC TGTCAGAGGT TCTGAGCTGC 
TCACAGAAGC TGGTCTGCTC TCTTGGCCAC TTAGGGAGTG TGAGTGAAGC CAAGGCCTTT 
TGCTTGGATG CCCTGAAACT GACGACAAAG CTGCAGCTCC CGGGCCAGTG TGCCCTGTTC 
CTGGTGCTGA AAGGTGAGCT GGAACTGGCC CGCAGCGACC TTGACCTCTG TCAGTCGGAC 
CTGCAGCAGG TTCTGTTCCT GCTTGAGTCT TGTACGGAGT TTGCCGGAGT GACCCAGCAC 
CCAGATACTA TAAAGAAGGT CCACCTACAG AAGGGGAAGC AGCAGGCCAG GGTCCCCTGG 
CCCCCGCAGC TCCTGGAGGA GGAGCCTTTC CTAAAAGGTC CTGCTCTGGA GCTGGTGGCC 
ACTGTAGCCA AGGAACCTGG GCCCATTGCA CCTTCGACAA ACTCCTCCCC CATCCTGAAA 
ACCAAGCCCC AACCCCGCCC CGGCTTCCTG TCTCACTCTG CTTCATGTGA CTGCTCGATC 
TGTGCCAGCC CTGTCCTGTC AGCAGTCTGT CTGCGCTGGG TGTTGGTCAC AGCAGGCGTG 
AGGCTGGCCA CAGGCCATCA GGCTCAGGGT CTAGAGCTGC TGCAGGTTGT TCTAAAGGGT 
TGCCCTGCAG CCACCAAGCG CCTCACCCAA ACTCTCCAGG CTTCCCTGAA TCACAGAGCC 
CCTGCCTCTC CTGACCCAAG CCTTCTTGAT GAGCTCCTGG CTCAGGCATA CACACAGCTG 
GCGCTGGCAG GCCTGAAGCA GCCATCAAAC AAGAGCCTGC AGAAGATCTT AGAGTCAGGG 
CTGAAGTTCA CAGCAGCGCG GATCCAACAG CTGGAGCCCT GGCGAGCCAG CCTGCTCTTG 
ATTCAGGCCC TCGTAAAGCT GGCTGGCCTC AGCACCTGTA CTACCCAACT TTTTGCAAGC 
TCCTGGGGCT GGCAGCCACC ATTAATCAAA AGCCCCCCGG GCTCAGAGTC CTCTAAGATC 
CAGAGCCAAA AACATAGTGG ACGAGGCCAC CAGCAAGTAG CCTCTGCTCC CCTGCCCCTC 
CATAATGCCT CTCACAGAAG CCCAGAAGGT GGTGGACCAC CCTGTACACC TAAGCCTGCA 
GGCCGAGTGA GGCCGGCTGG CCCTCGTGTC CCCTTCACTG TGTTTGAAGA TGACTGCCCT 
ACGAAGAACA AGCCCGAGGT TCCCCAGGCC CCCCGGGTAC ATCAGAGGGT CCAGACACGC 
CTTAAGGTGA ACTTCAGCGA TGACAGTGAC TTGGAAGACC CTGTCTCTGC TGAGATACCG 
CTTGTTGAGG AACCTAAGAG ACGGGGCACT GCTTCCTCGG GCCGGGGCCG GGGCCGGGGC 
CGGGCTAGGA AGAGCCCAAG CTTGGCGATA GGTACAGTGG CTGCTCCAGG TAATACCCCA 
ATGAGTGGTA AGAACCGTAG AACCAAGAAG GTGGCATCAA GGCTCTGTGA AGAGCAGGGT 
CCCCAGACAG CCCCTTGTGG CCAGAACAAG CTGGGCCCTG AGCTCATGAG GACTATCCCT 
GAGGAGAAAC TGACTGACAA GTGCACGGAA ATGAGCTTTG AGACCCTTGG AGCCTCTGAC 
AGAGAAGACT CAGCTTCAGG TGGGAAGGCA CTGGTTCCAG GTGCTGATCC CCCCGTAGGT 
GAATGTGAGG TGTTGAGACG GGATTCCAGC AAGGAGGAGC TACCCATCCT GGAACCAGGC 
AAGGAAAGCA ACAGGGACGT CGGTGCTTGG CTCCAACCCC CCGTAACCAT TGGTTCTTCT 
ACCCTGGATT CCATGCGCGA CTCTCTGAGC ATTGCTTTCC GTGGGGTCAG TCATTGCCCT 
GCTAGTGGGC TCTATGCCCA CCTCTGCCGC CTCCTGGCCT TGTGCCTGGG CCACCGGGAT 
CCCTATGCTA CTGCCTTCCT CGTTGCCGAG TCTATCTCTA TTACCTGTCG CCACCAGCTA 
CTCACTCACC TCCACAGACT GCTCAGCAAG GCCCAGAAGC ATCAAGGACC ACTCAGACTG 
ACAGAGCAGC TGCAGCAGCT AACCCTCCAG GAGATGCCTG ATGATGTCCC TCTGGCCCGC 
ATCCAGCGCC TCTTTTCCTT CAGGGCTTCA GGAGCTGGTG CCTTTCCCCA GCCCGAGAAG 
CAGCATTTCC AGGAGCTCTT GGTGCTGCTC CCCAAGGGGG TGACTGTCTG CATGTTGGCT 
GTGTCCACCC TCCAGCCTGG AACTGTGGGC AACACTCTCC TACTGACAAG ACTGGAAAAG 
GACAACCCCC CAGTCACTGT GCAGATCCCC ACTGCCCAGG ATAAGTTCCC TCTGAGTTCA 
GCCCTGAGAG AATTTGACGC TATCCAGAAG GAACAGAAAG AGAACAGCAG CTGCACTGAC 
AAGCGAGCCT GGTGGACAGG CAGACTGGCC CTGGACCACA GAATGGAGGA CCTCATCACT 
TCCCTGGAGA AGCATGTGTT GGGCTGCTGG CGAGGGCTGC TGCTGCCCTC CAGTGAAGAA 
CCTGGCCCTG AGCATGAGGC CACCCGCCTG CAGGAGTTGC TGCAGGACTG TGGCTGGCAG 
TATCCCGACC CGACTCTGTT AAAAGTCTTG CTCAGTGGTG CCAGCACCCT CACAACCCAG 
GATATTCAGG CCCTGGCCTA TGGGCTGTGC CCGACCCAGC CAGCCAGAGC CCAGGAACTC 
CTGACTGAGG CAGCCAGGCG TCTACAGGGG CAGTCAGCAC CAAGCAATAA GCATCTTGTC 
TTGGTGCTGG ACAGGGACCT GCAGAAGCTG CCATGGGAAA GCATACCCTG CCTGCGAGCA 
CTGCCTGTCA CCCGGCTGCC CTCCTTCCAC TTCCTGCTCA GCTATTTCAT CCTCAAAGAG 
GCTGGGGCTG CATCAGTGCT GAGCCAGGGG GTGGATCCTC GGAGCACATT TTACGTCCTG 
AACCCTCACA ATAACCTGGC AAGCACAGAG GAGCAATTTC GAGCCAAGTT TAGCAGTGAA 
GCTGGCTGGA GAGGGGTGGT CGGGGAGGTG CCAAGCCCAG AACAGATGCA GATGGCCTTG 
ACAAAGAACG ACTTATATAT CTATGCAGGT CACGGGGCTG GTGCCCGCTT CCTTGATGGG 
CAGGCAGTAC TGCGGCTAAG CTGCCGGGCA GTGGCCTTGT TGTTTGGCTG CAGCAGCGCA 
GCCCTGGCCG TGCATGGAAA CCTGGAGGGG GCTGGCATTG TGCTCAAGTA CATCATGGCT 
GGCTGCCCCC TGTTTCTGGG AAATCTCTGG GATGTGACTG ACCGTGACAT TGACCGCTAC 
ACAGAAGCTC TCCTGCAGGG ATGGCTTGCA GCAGGCCCAG GGGCCCCTCT GCTCCACTAT 
GTCAGCCAGG CCCGCCAGGC TCCCCGACTC AAGTCTCTTA TTGGAGCTGC ACCTGTTGCC 
TATGGCTTAC CTGTCTTCCT TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espl1 ( XM_013159374.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013014828.1 Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X2, mRNA.

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CloneID	ODo37679
Clone ID Related Accession (Same CDS sequence)	XM_013159374.1 , XM_023567606.1
Accession Version	XM_013159374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	separin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176410.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAACT TCAAAGGAGT CAACTTTGGA ACTCTGCTTA GAAGCCAGAA GGATGCTGAA 
GAGTTGCTGT CTGACTTGAA GGAGTTCTTG TCCAAGCCTC CAGCAGGTTT TCCCAGTAGC 
CGATCTGACT CTGAGAGGAG ACAAACTTGT GATGCCATCC TGAGGGCTTG CAACCAACAG 
CTGACGGCCA AGCTGTCTTG CCCTCAACAC CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTATGTGAT GTCCACTCCC CAGCGCCCTC CACTCTACCT GGAACGCATT 
CTTTTCATTT TACTACGGAA TGTTGCTGCT CAGGGAAGCC CCATGGCCAT GCTCCACCTT 
GCCCAGCCTC TCCATGCCTG CTTCGTTCAG TGCTCTCGCC AAGCTGCCTC ACAGGACTAT 
GAGGCTGTGG CTTGGGGCAG CTTTTCTCTG CTTTGGAAGG GAGCAGAAGC ACTGTTGGAG 
CAGAGAGATG CCTTCTCAGC GCGGATGAAG GCCTTGAGCT TCCTTGTCCT CCTGGAGGAT 
GAAAGTACCC CGTGTGAGAT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCAGTAGCT 
GCCTATCAGC TGTTTGATCG CAGTGGTTGT GGTATAAACA AAGCAGATGC TGATTTCTTA 
TATGATTTGC TCTCCAAAAA TGTGATCAGA ACCTTGGTGG GTGAGGAAAG TGGCTCTCCC 
AGTCCTCTTT CTTCCCAAAG GGCCCTCTGC CTCTTGGAGC TCACCTTAGA ACACTGCCGT 
CGCCTCTGCT TTAGCCACCA CTATGACAAG GCCACCAGTG CAGTTGAGAA GGCTCGTGGT 
TACCTAAGGG ACACCAGTGT AGCCTCCAGC CTCCAGCTAT GCCAGTTGGG GGTTGAGCTG 
CTACATGTTG GGGTGGAAGG ATCCCAGGCA GTGGCCCAGC TTCTCATCAA GGCATCAGCT 
GTCTTGAATA ACAGTTTGAA GGCCCCATCA CCTCCACTTC GGGCATTGTT TGAGACCTCC 
CAGTTCCTCC TTTCAGGCCT GGAACGAGGC ATCAAAAGAC ACTCTGGACC TGATGCTATT 
GGGAGCCTCT TCACTTTTCT TGGAGGATAC TGCTTAATTA TCCAGCAGCT ACGGAATGAT 
GGTGCCTGTG TGCACTCTCC CAAACAGCAG CAGTCTTTGG TGCAGACGCA CTTTCAGGGT 
CTTCACCTCT ACACTATGAT GGTTTATGAC TTTGCCCAGC GCTGTCAGGT AGCTGATTTG 
GCTGACCTGG CTCAACTAGT GGACAGTTGC AGATCTACTG CTGTTTGGAT GCTGGAAGCC 
TTGGAAGGCC TGTCAGGCCT GGAGCTGACT GACTACATGG CCATGACTGC CTCTTACACC 
AGTAACTTGG CCTATAGCTT CTACAATCAC AAGCTGTACG CCGAGGCCTG TGCCGTCTCT 
GAGCCACTCT GTCAGCACCT GGGCTCTGCT AAGCCAGGCA CTTACCATGC GGTGCCTTCT 
GAGAAGTTGC ACAGGTACTT CCGGCTACAC GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCACAGGGCT GTGAGATGGT GACCTTGTGG CTGGCGGCTC TACAGCCCTG CAACCCTGAA 
CACATGGCTG AGCCCGTCTC TTTCTGGGTT CGGGTAAAGA TGGATGCGAC CAGGGCTGGA 
AACAAGGAGC TACAGCTGAA GACTCTGCGA GACAGCCTGA GTGACTGGAA CCCAGAGACC 
CTGACCCTCC TGCTAAGGGA GGAGCTGCAG GCCTACAAGG CAGTACGGGC TGACACTGGG 
CAGGAACGTT TCAATGTCAT CTGTGACTTG CTGGAGCTGA GCCCTGAGGA GACACCAGCT 
GGGGCCTGGG CTCGAGCCAC CTACCTGGTG GAACTGGCTC AGGTGCTTTG CTACCACGAC 
TTTGCCCAGC AGACAGACTG CTCTGCCCTG GATGCTATCC AGGAAGCCCT GCAGCTTCTG 
GAGGCTGTTA AGCCAGAGGC CCAGGCCAAG GATCGGCTTC TGGACGATAA AGCACAGGCA 
CTGTTGTGGT TCTACATCTG CACTTTGGAG GCCAAAATGC GGGAAGGTAT TGAGCGGGAT 
CGGAGAGCCC AGGCTCCTAG TAACCTGGAG GAATTTGAAG TTAATGATCT CAACTATGAA 
GATAAACTCC AGGAAGATTG TTTCCTGTAC AGTAACATTG CCTTTAACCT GGCTGCAGAT 
GCAGCTCACT CTAAAGCCCT GGACCAAGCC CTGGCTCTGT GGAAGGAGGT CCTTACAGAG 
GGGCAGTTCC CAGCTGTGCG ATGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTTGTG 
GCCCTCTATC AGCTGGTGGC CAAGCCTCTG CAGGCCCTGG AGGCTCTCCT GCTCCTGCGG 
ACCATCTCTG AAAGACTGAA GGATCACGCA AAGGCAGCTA GCTCCTCTTG CCACCTCACC 
CAGGTCCTCC TGAGCCTCGG CTGTCCCACC TATGCCCAGT TTTACCTGGA AGAGGCTGAA 
TCCGCCCTGA TGCATCTCAG TGAGACCACG GACACACGAC TGCTGCTTTC TCTGACGTGT 
CACCTCCTTC GAAGTCAGCT CTGCTGGACT CAACAGAAGG TGACTGAGGG TGTCTCTCTG 
TTGCTGACTG TCCTTCGGGA TCCTGCCCTC CAGAAGTCAT CCAAGGCTTG GTATTTGCTT 
CGAGTCCAGG CTCTGCAACT ACTGTCCATC TACCTTAACC TCTCATCAAA CAGCCTCTCA 
GATGCTCTGC GAGAGCAGCT TTGGGCTCAA GGCTGGCAGA CACCTGAGAC AGCACTCATA 
GATTCTCATA AGCTCCTTCG AAGCATCATC CTCCTGCTGA TGGGCAGCGA TGTCCTCTCC 
CTACAGAAAG CAGCTGTGGA GACACCATTT CTGGATTGTG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TGTCAGAGGT TCTGAGCTGC TCACAGAAGC TGGTCTGCTC TCTTGGCCAC 
TTAGGGAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGATG CCCTGAAACT GACGACAAAG 
CTGCAGCTCC CGGGCCAGTG TGCCCTGTTC CTGGTGCTGA AAGGTGAGCT GGAACTGGCC 
CGCAGCGACC TTGACCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCCT GCTTGAGTCT 
TGTACGGAGT TTGCCGGAGT GACCCAGCAC CCAGATACTA TAAAGAAGGT CCACCTACAG 
AAGGGGAAGC AGCAGGCCAG GGTCCCCTGG CCCCCGCAGC TCCTGGAGGA GGAGCCTTTC 
CTAAAAGGTC CTGCTCTGGA GCTGGTGGCC ACTGTAGCCA AGGAACCTGG GCCCATTGCA 
CCTTCGACAA ACTCCTCCCC CATCCTGAAA ACCAAGCCCC AACCCCGCCC CGGCTTCCTG 
TCTCACTCTG CTTCATGTGA CTGCTCGATC TGTGCCAGCC CTGTCCTGTC AGCAGTCTGT 
CTGCGCTGGG TGTTGGTCAC AGCAGGCGTG AGGCTGGCCA CAGGCCATCA GGCTCAGGGT 
CTAGAGCTGC TGCAGGTTGT TCTAAAGGGT TGCCCTGCAG CCACCAAGCG CCTCACCCAA 
ACTCTCCAGG CTTCCCTGAA TCACAGAGCC CCTGCCTCTC CTGACCCAAG CCTTCTTGAT 
GAGCTCCTGG CTCAGGCATA CACACAGCTG GCGCTGGCAG GCCTGAAGCA GCCATCAAAC 
AAGAGCCTGC AGAAGATCTT AGAGTCAGGG CTGAAGTTCA CAGCAGCGCG GATCCAACAG 
CTGGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTCAGGCCC TCGTAAAGCT GGCTGGCCTC 
AGCACCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATCAAA 
AGCCCCCCGG GCTCAGAGTC CTCTAAGATC CAGAGCCAAA AACATAGTGG ACGAGGCCAC 
CAGCAAGTAG CCTCTGCTCC CCTGCCCCTC CATAATGCCT CTCACAGAAG CCCAGAAGGT 
GGTGGACCAC CCTGTACACC TAAGCCTGCA GGCCGAGTGA GGCCGGCTGG CCCTCGTGTC 
CCCTTCACTG TGTTTGAAGA TGACTGCCCT ACGAAGAACA AGCCCGAGGT TCCCCAGGCC 
CCCCGGGTAC ATCAGAGGGT CCAGACACGC CTTAAGGTGA ACTTCAGCGA TGACAGTGAC 
TTGGAAGACC CTGTCTCTGC TGAGATACCG CTTGTTGAGG AACCTAAGAG ACGGGGCACT 
GCTTCCTCGG GCCGGGGCCG GGGCCGGGGC CGGGCTAGGA AGAGCCCAAG CTTGGCGATA 
GGTACAGTGG CTGCTCCAGG TAATACCCCA ATGAGTGGTA AGAACCGTAG AACCAAGAAG 
GTGGCATCAA GGCTCTGTGA AGAGCAGGGT CCCCAGACAG CCCCTTGTGG CCAGAACAAG 
CTGGGCCCTG AGCTCATGAG GACTATCCCT GAGGAGAAAC TGACTGACAA GTGCACGGAA 
ATGAGCTTTG AGACCCTTGG AGCCTCTGAC AGAGAAGACT CAGCTTCAGG TGGGAAGGCA 
CTGGTTCCAG GTGCTGATCC CCCCGTAGGT GAATGTGAGG TGTTGAGACG GGATTCCAGC 
AAGGAGGAGC TACCCATCCT GGAACCAGGC AAGGAAAGCA ACAGGGACGT CGGTGCTTGG 
CTCCAACCCC CCGTAACCAT TGGTTCTTCT ACCCTGGATT CCATGCGCGA CTCTCTGAGC 
ATTGCTTTCC GTGGGGTCAG TCATTGCCCT GCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
CTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCCTATGCTA CTGCCTTCCT CGTTGCCGAG 
TCTATCTCTA TTACCTGTCG CCACCAGCTA CTCACTCACC TCCACAGACT GCTCAGCAAG 
GCCCAGAAGC ATCAAGGACC ACTCAGACTG ACAGAGCAGC TGCAGCAGCT AACCCTCCAG 
GAGATGCCTG ATGATGTCCC TCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTCA 
GGAGCTGGTG CCTTTCCCCA GCCCGAGAAG CAGCATTTCC AGGAGCTCTT GGTGCTGCTC 
CCCAAGGGGG TGACTGTCTG CATGTTGGCT GTGTCCACCC TCCAGCCTGG AACTGTGGGC 
AACACTCTCC TACTGACAAG ACTGGAAAAG GACAACCCCC CAGTCACTGT GCAGATCCCC 
ACTGCCCAGG ATAAGTTCCC TCTGAGTTCA GCCCTGAGAG AATTTGACGC TATCCAGAAG 
GAACAGAAAG AGAACAGCAG CTGCACTGAC AAGCGAGCCT GGTGGACAGG CAGACTGGCC 
CTGGACCACA GAATGGAGGA CCTCATCACT TCCCTGGAGA AGCATGTGTT GGGCTGCTGG 
CGAGGGCTGC TGCTGCCCTC CAGTGAAGAA CCTGGCCCTG AGCATGAGGC CACCCGCCTG 
CAGGAGTTGC TGCAGGACTG TGGCTGGCAG TATCCCGACC CGACTCTGTT AAAAGTCTTG 
CTCAGTGGTG CCAGCACCCT CACAACCCAG GATATTCAGG CCCTGGCCTA TGGGCTGTGC 
CCGACCCAGC CAGCCAGAGC CCAGGAACTC CTGACTGAGG CAGCCAGGCG TCTACAGGGG 
CAGTCAGCAC CAAGCAATAA GCATCTTGTC TTGGTGCTGG ACAGGGACCT GCAGAAGCTG 
CCATGGGAAA GCATACCCTG CCTGCGAGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCAC 
TTCCTGCTCA GCTATTTCAT CCTCAAAGAG GCTGGGGCTG CATCAGTGCT GAGCCAGGGG 
GTGGATCCTC GGAGCACATT TTACGTCCTG AACCCTCACA ATAACCTGGC AAGCACAGAG 
GAGCAATTTC GAGCCAAGTT TAGCAGTGAA GCTGGCTGGA GAGGGGTGGT CGGGGAGGTG 
CCAAGCCCAG AACAGATGCA GATGGCCTTG ACAAAGAACG ACTTATATAT CTATGCAGGT 
CACGGGGCTG GTGCCCGCTT CCTTGATGGG CAGGCAGTAC TGCGGCTAAG CTGCCGGGCA 
GTGGCCTTGT TGTTTGGCTG CAGCAGCGCA GCCCTGGCCG TGCATGGAAA CCTGGAGGGG 
GCTGGCATTG TGCTCAAGTA CATCATGGCT GGCTGCCCCC TGTTTCTGGG AAATCTCTGG 
GATGTGACTG ACCGTGACAT TGACCGCTAC ACAGAAGCTC TCCTGCAGGG ATGGCTTGCA 
GCAGGCCCAG GGGCCCCTCT GCTCCACTAT GTCAGCCAGG CCCGCCAGGC TCCCCGACTC 
AAGTCTCTTA TTGGAGCTGC ACCTGTTGCC TATGGCTTAC CTGTCTTCCT TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013014828.1
CDS	889..7245
Translation	MRNFKGVNFGTLLRSQKDAEELLSDLKEFLSKPPAGFPSSRSDSERRQTCDAILRACNQQLTAKLSCPQHLGSLLELAELACDGYVMSTPQRPPLYLERILFILLRNVAAQGSPMAMLHLAQPLHACFVQCSRQAASQDYEAVAWGSFSLLWKGAEALLEQRDAFSARMKALSFLVLLEDESTPCEIPHFASPTACRAVAAYQLFDRSGCGINKADADFLYDLLSKNVIRTLVGEESGSPSPLSSQRALCLLELTLEHCRRLCFSHHYDKATSAVEKARGYLRDTSVASSLQLCQLGVELLHVGVEGSQAVAQLLIKASAVLNNSLKAPSPPLRALFETSQFLLSGLERGIKRHSGPDAIGSLFTFLGGYCLIIQQLRNDGACVHSPKQQQSLVQTHFQGLHLYTMMVYDFAQRCQVADLADLAQLVDSCRSTAVWMLEALEGLSGLELTDYMAMTASYTSNLAYSFYNHKLYAEACAVSEPLCQHLGSAKPGTYHAVPSEKLHRYFRLHVESLKKLGKQAQGCEMVTLWLAALQPCNPEHMAEPVSFWVRVKMDATRAGNKELQLKTLRDSLSDWNPETLTLLLREELQAYKAVRADTGQERFNVICDLLELSPEETPAGAWARATYLVELAQVLCYHDFAQQTDCSALDAIQEALQLLEAVKPEAQAKDRLLDDKAQALLWFYICTLEAKMREGIERDRRAQAPSNLEEFEVNDLNYEDKLQEDCFLYSNIAFNLAADAAHSKALDQALALWKEVLTEGQFPAVRCLQQTAASLQILVALYQLVAKPLQALEALLLLRTISERLKDHAKAASSSCHLTQVLLSLGCPTYAQFYLEEAESALMHLSETTDTRLLLSLTCHLLRSQLCWTQQKVTEGVSLLLTVLRDPALQKSSKAWYLLRVQALQLLSIYLNLSSNSLSDALREQLWAQGWQTPETALIDSHKLLRSIILLLMGSDVLSLQKAAVETPFLDCGENLVQKWQVLSEVLSCSQKLVCSLGHLGSVSEAKAFCLDALKLTTKLQLPGQCALFLVLKGELELARSDLDLCQSDLQQVLFLLESCTEFAGVTQHPDTIKKVHLQKGKQQARVPWPPQLLEEEPFLKGPALELVATVAKEPGPIAPSTNSSPILKTKPQPRPGFLSHSASCDCSICASPVLSAVCLRWVLVTAGVRLATGHQAQGLELLQVVLKGCPAATKRLTQTLQASLNHRAPASPDPSLLDELLAQAYTQLALAGLKQPSNKSLQKILESGLKFTAARIQQLEPWRASLLLIQALVKLAGLSTCTTQLFASSWGWQPPLIKSPPGSESSKIQSQKHSGRGHQQVASAPLPLHNASHRSPEGGGPPCTPKPAGRVRPAGPRVPFTVFEDDCPTKNKPEVPQAPRVHQRVQTRLKVNFSDDSDLEDPVSAEIPLVEEPKRRGTASSGRGRGRGRARKSPSLAIGTVAAPGNTPMSGKNRRTKKVASRLCEEQGPQTAPCGQNKLGPELMRTIPEEKLTDKCTEMSFETLGASDREDSASGGKALVPGADPPVGECEVLRRDSSKEELPILEPGKESNRDVGAWLQPPVTIGSSTLDSMRDSLSIAFRGVSHCPASGLYAHLCRLLALCLGHRDPYATAFLVAESISITCRHQLLTHLHRLLSKAQKHQGPLRLTEQLQQLTLQEMPDDVPLARIQRLFSFRASGAGAFPQPEKQHFQELLVLLPKGVTVCMLAVSTLQPGTVGNTLLLTRLEKDNPPVTVQIPTAQDKFPLSSALREFDAIQKEQKENSSCTDKRAWWTGRLALDHRMEDLITSLEKHVLGCWRGLLLPSSEEPGPEHEATRLQELLQDCGWQYPDPTLLKVLLSGASTLTTQDIQALAYGLCPTQPARAQELLTEAARRLQGQSAPSNKHLVLVLDRDLQKLPWESIPCLRALPVTRLPSFHFLLSYFILKEAGAASVLSQGVDPRSTFYVLNPHNNLASTEEQFRAKFSSEAGWRGVVGEVPSPEQMQMALTKNDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLAAGPGAPLLHYVSQARQAPRLKSLIGAAPVAYGLPVFLR

Target ORF information:

RefSeq Version	XM_013159374.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013159374.1

ORF Insert Sequence:

ATGAGGAACT TCAAAGGAGT CAACTTTGGA ACTCTGCTTA GAAGCCAGAA GGATGCTGAA 
GAGTTGCTGT CTGACTTGAA GGAGTTCTTG TCCAAGCCTC CAGCAGGTTT TCCCAGTAGC 
CGATCTGACT CTGAGAGGAG ACAAACTTGT GATGCCATCC TGAGGGCTTG CAACCAACAG 
CTGACGGCCA AGCTGTCTTG CCCTCAACAC CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTATGTGAT GTCCACTCCC CAGCGCCCTC CACTCTACCT GGAACGCATT 
CTTTTCATTT TACTACGGAA TGTTGCTGCT CAGGGAAGCC CCATGGCCAT GCTCCACCTT 
GCCCAGCCTC TCCATGCCTG CTTCGTTCAG TGCTCTCGCC AAGCTGCCTC ACAGGACTAT 
GAGGCTGTGG CTTGGGGCAG CTTTTCTCTG CTTTGGAAGG GAGCAGAAGC ACTGTTGGAG 
CAGAGAGATG CCTTCTCAGC GCGGATGAAG GCCTTGAGCT TCCTTGTCCT CCTGGAGGAT 
GAAAGTACCC CGTGTGAGAT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCAGTAGCT 
GCCTATCAGC TGTTTGATCG CAGTGGTTGT GGTATAAACA AAGCAGATGC TGATTTCTTA 
TATGATTTGC TCTCCAAAAA TGTGATCAGA ACCTTGGTGG GTGAGGAAAG TGGCTCTCCC 
AGTCCTCTTT CTTCCCAAAG GGCCCTCTGC CTCTTGGAGC TCACCTTAGA ACACTGCCGT 
CGCCTCTGCT TTAGCCACCA CTATGACAAG GCCACCAGTG CAGTTGAGAA GGCTCGTGGT 
TACCTAAGGG ACACCAGTGT AGCCTCCAGC CTCCAGCTAT GCCAGTTGGG GGTTGAGCTG 
CTACATGTTG GGGTGGAAGG ATCCCAGGCA GTGGCCCAGC TTCTCATCAA GGCATCAGCT 
GTCTTGAATA ACAGTTTGAA GGCCCCATCA CCTCCACTTC GGGCATTGTT TGAGACCTCC 
CAGTTCCTCC TTTCAGGCCT GGAACGAGGC ATCAAAAGAC ACTCTGGACC TGATGCTATT 
GGGAGCCTCT TCACTTTTCT TGGAGGATAC TGCTTAATTA TCCAGCAGCT ACGGAATGAT 
GGTGCCTGTG TGCACTCTCC CAAACAGCAG CAGTCTTTGG TGCAGACGCA CTTTCAGGGT 
CTTCACCTCT ACACTATGAT GGTTTATGAC TTTGCCCAGC GCTGTCAGGT AGCTGATTTG 
GCTGACCTGG CTCAACTAGT GGACAGTTGC AGATCTACTG CTGTTTGGAT GCTGGAAGCC 
TTGGAAGGCC TGTCAGGCCT GGAGCTGACT GACTACATGG CCATGACTGC CTCTTACACC 
AGTAACTTGG CCTATAGCTT CTACAATCAC AAGCTGTACG CCGAGGCCTG TGCCGTCTCT 
GAGCCACTCT GTCAGCACCT GGGCTCTGCT AAGCCAGGCA CTTACCATGC GGTGCCTTCT 
GAGAAGTTGC ACAGGTACTT CCGGCTACAC GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCACAGGGCT GTGAGATGGT GACCTTGTGG CTGGCGGCTC TACAGCCCTG CAACCCTGAA 
CACATGGCTG AGCCCGTCTC TTTCTGGGTT CGGGTAAAGA TGGATGCGAC CAGGGCTGGA 
AACAAGGAGC TACAGCTGAA GACTCTGCGA GACAGCCTGA GTGACTGGAA CCCAGAGACC 
CTGACCCTCC TGCTAAGGGA GGAGCTGCAG GCCTACAAGG CAGTACGGGC TGACACTGGG 
CAGGAACGTT TCAATGTCAT CTGTGACTTG CTGGAGCTGA GCCCTGAGGA GACACCAGCT 
GGGGCCTGGG CTCGAGCCAC CTACCTGGTG GAACTGGCTC AGGTGCTTTG CTACCACGAC 
TTTGCCCAGC AGACAGACTG CTCTGCCCTG GATGCTATCC AGGAAGCCCT GCAGCTTCTG 
GAGGCTGTTA AGCCAGAGGC CCAGGCCAAG GATCGGCTTC TGGACGATAA AGCACAGGCA 
CTGTTGTGGT TCTACATCTG CACTTTGGAG GCCAAAATGC GGGAAGGTAT TGAGCGGGAT 
CGGAGAGCCC AGGCTCCTAG TAACCTGGAG GAATTTGAAG TTAATGATCT CAACTATGAA 
GATAAACTCC AGGAAGATTG TTTCCTGTAC AGTAACATTG CCTTTAACCT GGCTGCAGAT 
GCAGCTCACT CTAAAGCCCT GGACCAAGCC CTGGCTCTGT GGAAGGAGGT CCTTACAGAG 
GGGCAGTTCC CAGCTGTGCG ATGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTTGTG 
GCCCTCTATC AGCTGGTGGC CAAGCCTCTG CAGGCCCTGG AGGCTCTCCT GCTCCTGCGG 
ACCATCTCTG AAAGACTGAA GGATCACGCA AAGGCAGCTA GCTCCTCTTG CCACCTCACC 
CAGGTCCTCC TGAGCCTCGG CTGTCCCACC TATGCCCAGT TTTACCTGGA AGAGGCTGAA 
TCCGCCCTGA TGCATCTCAG TGAGACCACG GACACACGAC TGCTGCTTTC TCTGACGTGT 
CACCTCCTTC GAAGTCAGCT CTGCTGGACT CAACAGAAGG TGACTGAGGG TGTCTCTCTG 
TTGCTGACTG TCCTTCGGGA TCCTGCCCTC CAGAAGTCAT CCAAGGCTTG GTATTTGCTT 
CGAGTCCAGG CTCTGCAACT ACTGTCCATC TACCTTAACC TCTCATCAAA CAGCCTCTCA 
GATGCTCTGC GAGAGCAGCT TTGGGCTCAA GGCTGGCAGA CACCTGAGAC AGCACTCATA 
GATTCTCATA AGCTCCTTCG AAGCATCATC CTCCTGCTGA TGGGCAGCGA TGTCCTCTCC 
CTACAGAAAG CAGCTGTGGA GACACCATTT CTGGATTGTG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TGTCAGAGGT TCTGAGCTGC TCACAGAAGC TGGTCTGCTC TCTTGGCCAC 
TTAGGGAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGATG CCCTGAAACT GACGACAAAG 
CTGCAGCTCC CGGGCCAGTG TGCCCTGTTC CTGGTGCTGA AAGGTGAGCT GGAACTGGCC 
CGCAGCGACC TTGACCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCCT GCTTGAGTCT 
TGTACGGAGT TTGCCGGAGT GACCCAGCAC CCAGATACTA TAAAGAAGGT CCACCTACAG 
AAGGGGAAGC AGCAGGCCAG GGTCCCCTGG CCCCCGCAGC TCCTGGAGGA GGAGCCTTTC 
CTAAAAGGTC CTGCTCTGGA GCTGGTGGCC ACTGTAGCCA AGGAACCTGG GCCCATTGCA 
CCTTCGACAA ACTCCTCCCC CATCCTGAAA ACCAAGCCCC AACCCCGCCC CGGCTTCCTG 
TCTCACTCTG CTTCATGTGA CTGCTCGATC TGTGCCAGCC CTGTCCTGTC AGCAGTCTGT 
CTGCGCTGGG TGTTGGTCAC AGCAGGCGTG AGGCTGGCCA CAGGCCATCA GGCTCAGGGT 
CTAGAGCTGC TGCAGGTTGT TCTAAAGGGT TGCCCTGCAG CCACCAAGCG CCTCACCCAA 
ACTCTCCAGG CTTCCCTGAA TCACAGAGCC CCTGCCTCTC CTGACCCAAG CCTTCTTGAT 
GAGCTCCTGG CTCAGGCATA CACACAGCTG GCGCTGGCAG GCCTGAAGCA GCCATCAAAC 
AAGAGCCTGC AGAAGATCTT AGAGTCAGGG CTGAAGTTCA CAGCAGCGCG GATCCAACAG 
CTGGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTCAGGCCC TCGTAAAGCT GGCTGGCCTC 
AGCACCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATCAAA 
AGCCCCCCGG GCTCAGAGTC CTCTAAGATC CAGAGCCAAA AACATAGTGG ACGAGGCCAC 
CAGCAAGTAG CCTCTGCTCC CCTGCCCCTC CATAATGCCT CTCACAGAAG CCCAGAAGGT 
GGTGGACCAC CCTGTACACC TAAGCCTGCA GGCCGAGTGA GGCCGGCTGG CCCTCGTGTC 
CCCTTCACTG TGTTTGAAGA TGACTGCCCT ACGAAGAACA AGCCCGAGGT TCCCCAGGCC 
CCCCGGGTAC ATCAGAGGGT CCAGACACGC CTTAAGGTGA ACTTCAGCGA TGACAGTGAC 
TTGGAAGACC CTGTCTCTGC TGAGATACCG CTTGTTGAGG AACCTAAGAG ACGGGGCACT 
GCTTCCTCGG GCCGGGGCCG GGGCCGGGGC CGGGCTAGGA AGAGCCCAAG CTTGGCGATA 
GGTACAGTGG CTGCTCCAGG TAATACCCCA ATGAGTGGTA AGAACCGTAG AACCAAGAAG 
GTGGCATCAA GGCTCTGTGA AGAGCAGGGT CCCCAGACAG CCCCTTGTGG CCAGAACAAG 
CTGGGCCCTG AGCTCATGAG GACTATCCCT GAGGAGAAAC TGACTGACAA GTGCACGGAA 
ATGAGCTTTG AGACCCTTGG AGCCTCTGAC AGAGAAGACT CAGCTTCAGG TGGGAAGGCA 
CTGGTTCCAG GTGCTGATCC CCCCGTAGGT GAATGTGAGG TGTTGAGACG GGATTCCAGC 
AAGGAGGAGC TACCCATCCT GGAACCAGGC AAGGAAAGCA ACAGGGACGT CGGTGCTTGG 
CTCCAACCCC CCGTAACCAT TGGTTCTTCT ACCCTGGATT CCATGCGCGA CTCTCTGAGC 
ATTGCTTTCC GTGGGGTCAG TCATTGCCCT GCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
CTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCCTATGCTA CTGCCTTCCT CGTTGCCGAG 
TCTATCTCTA TTACCTGTCG CCACCAGCTA CTCACTCACC TCCACAGACT GCTCAGCAAG 
GCCCAGAAGC ATCAAGGACC ACTCAGACTG ACAGAGCAGC TGCAGCAGCT AACCCTCCAG 
GAGATGCCTG ATGATGTCCC TCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTCA 
GGAGCTGGTG CCTTTCCCCA GCCCGAGAAG CAGCATTTCC AGGAGCTCTT GGTGCTGCTC 
CCCAAGGGGG TGACTGTCTG CATGTTGGCT GTGTCCACCC TCCAGCCTGG AACTGTGGGC 
AACACTCTCC TACTGACAAG ACTGGAAAAG GACAACCCCC CAGTCACTGT GCAGATCCCC 
ACTGCCCAGG ATAAGTTCCC TCTGAGTTCA GCCCTGAGAG AATTTGACGC TATCCAGAAG 
GAACAGAAAG AGAACAGCAG CTGCACTGAC AAGCGAGCCT GGTGGACAGG CAGACTGGCC 
CTGGACCACA GAATGGAGGA CCTCATCACT TCCCTGGAGA AGCATGTGTT GGGCTGCTGG 
CGAGGGCTGC TGCTGCCCTC CAGTGAAGAA CCTGGCCCTG AGCATGAGGC CACCCGCCTG 
CAGGAGTTGC TGCAGGACTG TGGCTGGCAG TATCCCGACC CGACTCTGTT AAAAGTCTTG 
CTCAGTGGTG CCAGCACCCT CACAACCCAG GATATTCAGG CCCTGGCCTA TGGGCTGTGC 
CCGACCCAGC CAGCCAGAGC CCAGGAACTC CTGACTGAGG CAGCCAGGCG TCTACAGGGG 
CAGTCAGCAC CAAGCAATAA GCATCTTGTC TTGGTGCTGG ACAGGGACCT GCAGAAGCTG 
CCATGGGAAA GCATACCCTG CCTGCGAGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCAC 
TTCCTGCTCA GCTATTTCAT CCTCAAAGAG GCTGGGGCTG CATCAGTGCT GAGCCAGGGG 
GTGGATCCTC GGAGCACATT TTACGTCCTG AACCCTCACA ATAACCTGGC AAGCACAGAG 
GAGCAATTTC GAGCCAAGTT TAGCAGTGAA GCTGGCTGGA GAGGGGTGGT CGGGGAGGTG 
CCAAGCCCAG AACAGATGCA GATGGCCTTG ACAAAGAACG ACTTATATAT CTATGCAGGT 
CACGGGGCTG GTGCCCGCTT CCTTGATGGG CAGGCAGTAC TGCGGCTAAG CTGCCGGGCA 
GTGGCCTTGT TGTTTGGCTG CAGCAGCGCA GCCCTGGCCG TGCATGGAAA CCTGGAGGGG 
GCTGGCATTG TGCTCAAGTA CATCATGGCT GGCTGCCCCC TGTTTCTGGG AAATCTCTGG 
GATGTGACTG ACCGTGACAT TGACCGCTAC ACAGAAGCTC TCCTGCAGGG ATGGCTTGCA 
GCAGGCCCAG GGGCCCCTCT GCTCCACTAT GTCAGCCAGG CCCGCCAGGC TCCCCGACTC 
AAGTCTCTTA TTGGAGCTGC ACCTGTTGCC TATGGCTTAC CTGTCTTCCT TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espl1 ( XM_023567606.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_023423374.1 Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X2, mRNA.

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CloneID	ODo37679
Clone ID Related Accession (Same CDS sequence)	XM_013159374.1 , XM_023567606.1
Accession Version	XM_023567606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	separin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176410.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAACT TCAAAGGAGT CAACTTTGGA ACTCTGCTTA GAAGCCAGAA GGATGCTGAA 
GAGTTGCTGT CTGACTTGAA GGAGTTCTTG TCCAAGCCTC CAGCAGGTTT TCCCAGTAGC 
CGATCTGACT CTGAGAGGAG ACAAACTTGT GATGCCATCC TGAGGGCTTG CAACCAACAG 
CTGACGGCCA AGCTGTCTTG CCCTCAACAC CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTATGTGAT GTCCACTCCC CAGCGCCCTC CACTCTACCT GGAACGCATT 
CTTTTCATTT TACTACGGAA TGTTGCTGCT CAGGGAAGCC CCATGGCCAT GCTCCACCTT 
GCCCAGCCTC TCCATGCCTG CTTCGTTCAG TGCTCTCGCC AAGCTGCCTC ACAGGACTAT 
GAGGCTGTGG CTTGGGGCAG CTTTTCTCTG CTTTGGAAGG GAGCAGAAGC ACTGTTGGAG 
CAGAGAGATG CCTTCTCAGC GCGGATGAAG GCCTTGAGCT TCCTTGTCCT CCTGGAGGAT 
GAAAGTACCC CGTGTGAGAT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCAGTAGCT 
GCCTATCAGC TGTTTGATCG CAGTGGTTGT GGTATAAACA AAGCAGATGC TGATTTCTTA 
TATGATTTGC TCTCCAAAAA TGTGATCAGA ACCTTGGTGG GTGAGGAAAG TGGCTCTCCC 
AGTCCTCTTT CTTCCCAAAG GGCCCTCTGC CTCTTGGAGC TCACCTTAGA ACACTGCCGT 
CGCCTCTGCT TTAGCCACCA CTATGACAAG GCCACCAGTG CAGTTGAGAA GGCTCGTGGT 
TACCTAAGGG ACACCAGTGT AGCCTCCAGC CTCCAGCTAT GCCAGTTGGG GGTTGAGCTG 
CTACATGTTG GGGTGGAAGG ATCCCAGGCA GTGGCCCAGC TTCTCATCAA GGCATCAGCT 
GTCTTGAATA ACAGTTTGAA GGCCCCATCA CCTCCACTTC GGGCATTGTT TGAGACCTCC 
CAGTTCCTCC TTTCAGGCCT GGAACGAGGC ATCAAAAGAC ACTCTGGACC TGATGCTATT 
GGGAGCCTCT TCACTTTTCT TGGAGGATAC TGCTTAATTA TCCAGCAGCT ACGGAATGAT 
GGTGCCTGTG TGCACTCTCC CAAACAGCAG CAGTCTTTGG TGCAGACGCA CTTTCAGGGT 
CTTCACCTCT ACACTATGAT GGTTTATGAC TTTGCCCAGC GCTGTCAGGT AGCTGATTTG 
GCTGACCTGG CTCAACTAGT GGACAGTTGC AGATCTACTG CTGTTTGGAT GCTGGAAGCC 
TTGGAAGGCC TGTCAGGCCT GGAGCTGACT GACTACATGG CCATGACTGC CTCTTACACC 
AGTAACTTGG CCTATAGCTT CTACAATCAC AAGCTGTACG CCGAGGCCTG TGCCGTCTCT 
GAGCCACTCT GTCAGCACCT GGGCTCTGCT AAGCCAGGCA CTTACCATGC GGTGCCTTCT 
GAGAAGTTGC ACAGGTACTT CCGGCTACAC GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCACAGGGCT GTGAGATGGT GACCTTGTGG CTGGCGGCTC TACAGCCCTG CAACCCTGAA 
CACATGGCTG AGCCCGTCTC TTTCTGGGTT CGGGTAAAGA TGGATGCGAC CAGGGCTGGA 
AACAAGGAGC TACAGCTGAA GACTCTGCGA GACAGCCTGA GTGACTGGAA CCCAGAGACC 
CTGACCCTCC TGCTAAGGGA GGAGCTGCAG GCCTACAAGG CAGTACGGGC TGACACTGGG 
CAGGAACGTT TCAATGTCAT CTGTGACTTG CTGGAGCTGA GCCCTGAGGA GACACCAGCT 
GGGGCCTGGG CTCGAGCCAC CTACCTGGTG GAACTGGCTC AGGTGCTTTG CTACCACGAC 
TTTGCCCAGC AGACAGACTG CTCTGCCCTG GATGCTATCC AGGAAGCCCT GCAGCTTCTG 
GAGGCTGTTA AGCCAGAGGC CCAGGCCAAG GATCGGCTTC TGGACGATAA AGCACAGGCA 
CTGTTGTGGT TCTACATCTG CACTTTGGAG GCCAAAATGC GGGAAGGTAT TGAGCGGGAT 
CGGAGAGCCC AGGCTCCTAG TAACCTGGAG GAATTTGAAG TTAATGATCT CAACTATGAA 
GATAAACTCC AGGAAGATTG TTTCCTGTAC AGTAACATTG CCTTTAACCT GGCTGCAGAT 
GCAGCTCACT CTAAAGCCCT GGACCAAGCC CTGGCTCTGT GGAAGGAGGT CCTTACAGAG 
GGGCAGTTCC CAGCTGTGCG ATGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTTGTG 
GCCCTCTATC AGCTGGTGGC CAAGCCTCTG CAGGCCCTGG AGGCTCTCCT GCTCCTGCGG 
ACCATCTCTG AAAGACTGAA GGATCACGCA AAGGCAGCTA GCTCCTCTTG CCACCTCACC 
CAGGTCCTCC TGAGCCTCGG CTGTCCCACC TATGCCCAGT TTTACCTGGA AGAGGCTGAA 
TCCGCCCTGA TGCATCTCAG TGAGACCACG GACACACGAC TGCTGCTTTC TCTGACGTGT 
CACCTCCTTC GAAGTCAGCT CTGCTGGACT CAACAGAAGG TGACTGAGGG TGTCTCTCTG 
TTGCTGACTG TCCTTCGGGA TCCTGCCCTC CAGAAGTCAT CCAAGGCTTG GTATTTGCTT 
CGAGTCCAGG CTCTGCAACT ACTGTCCATC TACCTTAACC TCTCATCAAA CAGCCTCTCA 
GATGCTCTGC GAGAGCAGCT TTGGGCTCAA GGCTGGCAGA CACCTGAGAC AGCACTCATA 
GATTCTCATA AGCTCCTTCG AAGCATCATC CTCCTGCTGA TGGGCAGCGA TGTCCTCTCC 
CTACAGAAAG CAGCTGTGGA GACACCATTT CTGGATTGTG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TGTCAGAGGT TCTGAGCTGC TCACAGAAGC TGGTCTGCTC TCTTGGCCAC 
TTAGGGAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGATG CCCTGAAACT GACGACAAAG 
CTGCAGCTCC CGGGCCAGTG TGCCCTGTTC CTGGTGCTGA AAGGTGAGCT GGAACTGGCC 
CGCAGCGACC TTGACCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCCT GCTTGAGTCT 
TGTACGGAGT TTGCCGGAGT GACCCAGCAC CCAGATACTA TAAAGAAGGT CCACCTACAG 
AAGGGGAAGC AGCAGGCCAG GGTCCCCTGG CCCCCGCAGC TCCTGGAGGA GGAGCCTTTC 
CTAAAAGGTC CTGCTCTGGA GCTGGTGGCC ACTGTAGCCA AGGAACCTGG GCCCATTGCA 
CCTTCGACAA ACTCCTCCCC CATCCTGAAA ACCAAGCCCC AACCCCGCCC CGGCTTCCTG 
TCTCACTCTG CTTCATGTGA CTGCTCGATC TGTGCCAGCC CTGTCCTGTC AGCAGTCTGT 
CTGCGCTGGG TGTTGGTCAC AGCAGGCGTG AGGCTGGCCA CAGGCCATCA GGCTCAGGGT 
CTAGAGCTGC TGCAGGTTGT TCTAAAGGGT TGCCCTGCAG CCACCAAGCG CCTCACCCAA 
ACTCTCCAGG CTTCCCTGAA TCACAGAGCC CCTGCCTCTC CTGACCCAAG CCTTCTTGAT 
GAGCTCCTGG CTCAGGCATA CACACAGCTG GCGCTGGCAG GCCTGAAGCA GCCATCAAAC 
AAGAGCCTGC AGAAGATCTT AGAGTCAGGG CTGAAGTTCA CAGCAGCGCG GATCCAACAG 
CTGGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTCAGGCCC TCGTAAAGCT GGCTGGCCTC 
AGCACCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATCAAA 
AGCCCCCCGG GCTCAGAGTC CTCTAAGATC CAGAGCCAAA AACATAGTGG ACGAGGCCAC 
CAGCAAGTAG CCTCTGCTCC CCTGCCCCTC CATAATGCCT CTCACAGAAG CCCAGAAGGT 
GGTGGACCAC CCTGTACACC TAAGCCTGCA GGCCGAGTGA GGCCGGCTGG CCCTCGTGTC 
CCCTTCACTG TGTTTGAAGA TGACTGCCCT ACGAAGAACA AGCCCGAGGT TCCCCAGGCC 
CCCCGGGTAC ATCAGAGGGT CCAGACACGC CTTAAGGTGA ACTTCAGCGA TGACAGTGAC 
TTGGAAGACC CTGTCTCTGC TGAGATACCG CTTGTTGAGG AACCTAAGAG ACGGGGCACT 
GCTTCCTCGG GCCGGGGCCG GGGCCGGGGC CGGGCTAGGA AGAGCCCAAG CTTGGCGATA 
GGTACAGTGG CTGCTCCAGG TAATACCCCA ATGAGTGGTA AGAACCGTAG AACCAAGAAG 
GTGGCATCAA GGCTCTGTGA AGAGCAGGGT CCCCAGACAG CCCCTTGTGG CCAGAACAAG 
CTGGGCCCTG AGCTCATGAG GACTATCCCT GAGGAGAAAC TGACTGACAA GTGCACGGAA 
ATGAGCTTTG AGACCCTTGG AGCCTCTGAC AGAGAAGACT CAGCTTCAGG TGGGAAGGCA 
CTGGTTCCAG GTGCTGATCC CCCCGTAGGT GAATGTGAGG TGTTGAGACG GGATTCCAGC 
AAGGAGGAGC TACCCATCCT GGAACCAGGC AAGGAAAGCA ACAGGGACGT CGGTGCTTGG 
CTCCAACCCC CCGTAACCAT TGGTTCTTCT ACCCTGGATT CCATGCGCGA CTCTCTGAGC 
ATTGCTTTCC GTGGGGTCAG TCATTGCCCT GCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
CTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCCTATGCTA CTGCCTTCCT CGTTGCCGAG 
TCTATCTCTA TTACCTGTCG CCACCAGCTA CTCACTCACC TCCACAGACT GCTCAGCAAG 
GCCCAGAAGC ATCAAGGACC ACTCAGACTG ACAGAGCAGC TGCAGCAGCT AACCCTCCAG 
GAGATGCCTG ATGATGTCCC TCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTCA 
GGAGCTGGTG CCTTTCCCCA GCCCGAGAAG CAGCATTTCC AGGAGCTCTT GGTGCTGCTC 
CCCAAGGGGG TGACTGTCTG CATGTTGGCT GTGTCCACCC TCCAGCCTGG AACTGTGGGC 
AACACTCTCC TACTGACAAG ACTGGAAAAG GACAACCCCC CAGTCACTGT GCAGATCCCC 
ACTGCCCAGG ATAAGTTCCC TCTGAGTTCA GCCCTGAGAG AATTTGACGC TATCCAGAAG 
GAACAGAAAG AGAACAGCAG CTGCACTGAC AAGCGAGCCT GGTGGACAGG CAGACTGGCC 
CTGGACCACA GAATGGAGGA CCTCATCACT TCCCTGGAGA AGCATGTGTT GGGCTGCTGG 
CGAGGGCTGC TGCTGCCCTC CAGTGAAGAA CCTGGCCCTG AGCATGAGGC CACCCGCCTG 
CAGGAGTTGC TGCAGGACTG TGGCTGGCAG TATCCCGACC CGACTCTGTT AAAAGTCTTG 
CTCAGTGGTG CCAGCACCCT CACAACCCAG GATATTCAGG CCCTGGCCTA TGGGCTGTGC 
CCGACCCAGC CAGCCAGAGC CCAGGAACTC CTGACTGAGG CAGCCAGGCG TCTACAGGGG 
CAGTCAGCAC CAAGCAATAA GCATCTTGTC TTGGTGCTGG ACAGGGACCT GCAGAAGCTG 
CCATGGGAAA GCATACCCTG CCTGCGAGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCAC 
TTCCTGCTCA GCTATTTCAT CCTCAAAGAG GCTGGGGCTG CATCAGTGCT GAGCCAGGGG 
GTGGATCCTC GGAGCACATT TTACGTCCTG AACCCTCACA ATAACCTGGC AAGCACAGAG 
GAGCAATTTC GAGCCAAGTT TAGCAGTGAA GCTGGCTGGA GAGGGGTGGT CGGGGAGGTG 
CCAAGCCCAG AACAGATGCA GATGGCCTTG ACAAAGAACG ACTTATATAT CTATGCAGGT 
CACGGGGCTG GTGCCCGCTT CCTTGATGGG CAGGCAGTAC TGCGGCTAAG CTGCCGGGCA 
GTGGCCTTGT TGTTTGGCTG CAGCAGCGCA GCCCTGGCCG TGCATGGAAA CCTGGAGGGG 
GCTGGCATTG TGCTCAAGTA CATCATGGCT GGCTGCCCCC TGTTTCTGGG AAATCTCTGG 
GATGTGACTG ACCGTGACAT TGACCGCTAC ACAGAAGCTC TCCTGCAGGG ATGGCTTGCA 
GCAGGCCCAG GGGCCCCTCT GCTCCACTAT GTCAGCCAGG CCCGCCAGGC TCCCCGACTC 
AAGTCTCTTA TTGGAGCTGC ACCTGTTGCC TATGGCTTAC CTGTCTTCCT TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023423374.1
CDS	157..6513
Translation	MRNFKGVNFGTLLRSQKDAEELLSDLKEFLSKPPAGFPSSRSDSERRQTCDAILRACNQQLTAKLSCPQHLGSLLELAELACDGYVMSTPQRPPLYLERILFILLRNVAAQGSPMAMLHLAQPLHACFVQCSRQAASQDYEAVAWGSFSLLWKGAEALLEQRDAFSARMKALSFLVLLEDESTPCEIPHFASPTACRAVAAYQLFDRSGCGINKADADFLYDLLSKNVIRTLVGEESGSPSPLSSQRALCLLELTLEHCRRLCFSHHYDKATSAVEKARGYLRDTSVASSLQLCQLGVELLHVGVEGSQAVAQLLIKASAVLNNSLKAPSPPLRALFETSQFLLSGLERGIKRHSGPDAIGSLFTFLGGYCLIIQQLRNDGACVHSPKQQQSLVQTHFQGLHLYTMMVYDFAQRCQVADLADLAQLVDSCRSTAVWMLEALEGLSGLELTDYMAMTASYTSNLAYSFYNHKLYAEACAVSEPLCQHLGSAKPGTYHAVPSEKLHRYFRLHVESLKKLGKQAQGCEMVTLWLAALQPCNPEHMAEPVSFWVRVKMDATRAGNKELQLKTLRDSLSDWNPETLTLLLREELQAYKAVRADTGQERFNVICDLLELSPEETPAGAWARATYLVELAQVLCYHDFAQQTDCSALDAIQEALQLLEAVKPEAQAKDRLLDDKAQALLWFYICTLEAKMREGIERDRRAQAPSNLEEFEVNDLNYEDKLQEDCFLYSNIAFNLAADAAHSKALDQALALWKEVLTEGQFPAVRCLQQTAASLQILVALYQLVAKPLQALEALLLLRTISERLKDHAKAASSSCHLTQVLLSLGCPTYAQFYLEEAESALMHLSETTDTRLLLSLTCHLLRSQLCWTQQKVTEGVSLLLTVLRDPALQKSSKAWYLLRVQALQLLSIYLNLSSNSLSDALREQLWAQGWQTPETALIDSHKLLRSIILLLMGSDVLSLQKAAVETPFLDCGENLVQKWQVLSEVLSCSQKLVCSLGHLGSVSEAKAFCLDALKLTTKLQLPGQCALFLVLKGELELARSDLDLCQSDLQQVLFLLESCTEFAGVTQHPDTIKKVHLQKGKQQARVPWPPQLLEEEPFLKGPALELVATVAKEPGPIAPSTNSSPILKTKPQPRPGFLSHSASCDCSICASPVLSAVCLRWVLVTAGVRLATGHQAQGLELLQVVLKGCPAATKRLTQTLQASLNHRAPASPDPSLLDELLAQAYTQLALAGLKQPSNKSLQKILESGLKFTAARIQQLEPWRASLLLIQALVKLAGLSTCTTQLFASSWGWQPPLIKSPPGSESSKIQSQKHSGRGHQQVASAPLPLHNASHRSPEGGGPPCTPKPAGRVRPAGPRVPFTVFEDDCPTKNKPEVPQAPRVHQRVQTRLKVNFSDDSDLEDPVSAEIPLVEEPKRRGTASSGRGRGRGRARKSPSLAIGTVAAPGNTPMSGKNRRTKKVASRLCEEQGPQTAPCGQNKLGPELMRTIPEEKLTDKCTEMSFETLGASDREDSASGGKALVPGADPPVGECEVLRRDSSKEELPILEPGKESNRDVGAWLQPPVTIGSSTLDSMRDSLSIAFRGVSHCPASGLYAHLCRLLALCLGHRDPYATAFLVAESISITCRHQLLTHLHRLLSKAQKHQGPLRLTEQLQQLTLQEMPDDVPLARIQRLFSFRASGAGAFPQPEKQHFQELLVLLPKGVTVCMLAVSTLQPGTVGNTLLLTRLEKDNPPVTVQIPTAQDKFPLSSALREFDAIQKEQKENSSCTDKRAWWTGRLALDHRMEDLITSLEKHVLGCWRGLLLPSSEEPGPEHEATRLQELLQDCGWQYPDPTLLKVLLSGASTLTTQDIQALAYGLCPTQPARAQELLTEAARRLQGQSAPSNKHLVLVLDRDLQKLPWESIPCLRALPVTRLPSFHFLLSYFILKEAGAASVLSQGVDPRSTFYVLNPHNNLASTEEQFRAKFSSEAGWRGVVGEVPSPEQMQMALTKNDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLAAGPGAPLLHYVSQARQAPRLKSLIGAAPVAYGLPVFLR

Target ORF information:

RefSeq Version	XM_023567606.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023567606.1

ORF Insert Sequence:

ATGAGGAACT TCAAAGGAGT CAACTTTGGA ACTCTGCTTA GAAGCCAGAA GGATGCTGAA 
GAGTTGCTGT CTGACTTGAA GGAGTTCTTG TCCAAGCCTC CAGCAGGTTT TCCCAGTAGC 
CGATCTGACT CTGAGAGGAG ACAAACTTGT GATGCCATCC TGAGGGCTTG CAACCAACAG 
CTGACGGCCA AGCTGTCTTG CCCTCAACAC CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTATGTGAT GTCCACTCCC CAGCGCCCTC CACTCTACCT GGAACGCATT 
CTTTTCATTT TACTACGGAA TGTTGCTGCT CAGGGAAGCC CCATGGCCAT GCTCCACCTT 
GCCCAGCCTC TCCATGCCTG CTTCGTTCAG TGCTCTCGCC AAGCTGCCTC ACAGGACTAT 
GAGGCTGTGG CTTGGGGCAG CTTTTCTCTG CTTTGGAAGG GAGCAGAAGC ACTGTTGGAG 
CAGAGAGATG CCTTCTCAGC GCGGATGAAG GCCTTGAGCT TCCTTGTCCT CCTGGAGGAT 
GAAAGTACCC CGTGTGAGAT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCAGTAGCT 
GCCTATCAGC TGTTTGATCG CAGTGGTTGT GGTATAAACA AAGCAGATGC TGATTTCTTA 
TATGATTTGC TCTCCAAAAA TGTGATCAGA ACCTTGGTGG GTGAGGAAAG TGGCTCTCCC 
AGTCCTCTTT CTTCCCAAAG GGCCCTCTGC CTCTTGGAGC TCACCTTAGA ACACTGCCGT 
CGCCTCTGCT TTAGCCACCA CTATGACAAG GCCACCAGTG CAGTTGAGAA GGCTCGTGGT 
TACCTAAGGG ACACCAGTGT AGCCTCCAGC CTCCAGCTAT GCCAGTTGGG GGTTGAGCTG 
CTACATGTTG GGGTGGAAGG ATCCCAGGCA GTGGCCCAGC TTCTCATCAA GGCATCAGCT 
GTCTTGAATA ACAGTTTGAA GGCCCCATCA CCTCCACTTC GGGCATTGTT TGAGACCTCC 
CAGTTCCTCC TTTCAGGCCT GGAACGAGGC ATCAAAAGAC ACTCTGGACC TGATGCTATT 
GGGAGCCTCT TCACTTTTCT TGGAGGATAC TGCTTAATTA TCCAGCAGCT ACGGAATGAT 
GGTGCCTGTG TGCACTCTCC CAAACAGCAG CAGTCTTTGG TGCAGACGCA CTTTCAGGGT 
CTTCACCTCT ACACTATGAT GGTTTATGAC TTTGCCCAGC GCTGTCAGGT AGCTGATTTG 
GCTGACCTGG CTCAACTAGT GGACAGTTGC AGATCTACTG CTGTTTGGAT GCTGGAAGCC 
TTGGAAGGCC TGTCAGGCCT GGAGCTGACT GACTACATGG CCATGACTGC CTCTTACACC 
AGTAACTTGG CCTATAGCTT CTACAATCAC AAGCTGTACG CCGAGGCCTG TGCCGTCTCT 
GAGCCACTCT GTCAGCACCT GGGCTCTGCT AAGCCAGGCA CTTACCATGC GGTGCCTTCT 
GAGAAGTTGC ACAGGTACTT CCGGCTACAC GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCACAGGGCT GTGAGATGGT GACCTTGTGG CTGGCGGCTC TACAGCCCTG CAACCCTGAA 
CACATGGCTG AGCCCGTCTC TTTCTGGGTT CGGGTAAAGA TGGATGCGAC CAGGGCTGGA 
AACAAGGAGC TACAGCTGAA GACTCTGCGA GACAGCCTGA GTGACTGGAA CCCAGAGACC 
CTGACCCTCC TGCTAAGGGA GGAGCTGCAG GCCTACAAGG CAGTACGGGC TGACACTGGG 
CAGGAACGTT TCAATGTCAT CTGTGACTTG CTGGAGCTGA GCCCTGAGGA GACACCAGCT 
GGGGCCTGGG CTCGAGCCAC CTACCTGGTG GAACTGGCTC AGGTGCTTTG CTACCACGAC 
TTTGCCCAGC AGACAGACTG CTCTGCCCTG GATGCTATCC AGGAAGCCCT GCAGCTTCTG 
GAGGCTGTTA AGCCAGAGGC CCAGGCCAAG GATCGGCTTC TGGACGATAA AGCACAGGCA 
CTGTTGTGGT TCTACATCTG CACTTTGGAG GCCAAAATGC GGGAAGGTAT TGAGCGGGAT 
CGGAGAGCCC AGGCTCCTAG TAACCTGGAG GAATTTGAAG TTAATGATCT CAACTATGAA 
GATAAACTCC AGGAAGATTG TTTCCTGTAC AGTAACATTG CCTTTAACCT GGCTGCAGAT 
GCAGCTCACT CTAAAGCCCT GGACCAAGCC CTGGCTCTGT GGAAGGAGGT CCTTACAGAG 
GGGCAGTTCC CAGCTGTGCG ATGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTTGTG 
GCCCTCTATC AGCTGGTGGC CAAGCCTCTG CAGGCCCTGG AGGCTCTCCT GCTCCTGCGG 
ACCATCTCTG AAAGACTGAA GGATCACGCA AAGGCAGCTA GCTCCTCTTG CCACCTCACC 
CAGGTCCTCC TGAGCCTCGG CTGTCCCACC TATGCCCAGT TTTACCTGGA AGAGGCTGAA 
TCCGCCCTGA TGCATCTCAG TGAGACCACG GACACACGAC TGCTGCTTTC TCTGACGTGT 
CACCTCCTTC GAAGTCAGCT CTGCTGGACT CAACAGAAGG TGACTGAGGG TGTCTCTCTG 
TTGCTGACTG TCCTTCGGGA TCCTGCCCTC CAGAAGTCAT CCAAGGCTTG GTATTTGCTT 
CGAGTCCAGG CTCTGCAACT ACTGTCCATC TACCTTAACC TCTCATCAAA CAGCCTCTCA 
GATGCTCTGC GAGAGCAGCT TTGGGCTCAA GGCTGGCAGA CACCTGAGAC AGCACTCATA 
GATTCTCATA AGCTCCTTCG AAGCATCATC CTCCTGCTGA TGGGCAGCGA TGTCCTCTCC 
CTACAGAAAG CAGCTGTGGA GACACCATTT CTGGATTGTG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TGTCAGAGGT TCTGAGCTGC TCACAGAAGC TGGTCTGCTC TCTTGGCCAC 
TTAGGGAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGATG CCCTGAAACT GACGACAAAG 
CTGCAGCTCC CGGGCCAGTG TGCCCTGTTC CTGGTGCTGA AAGGTGAGCT GGAACTGGCC 
CGCAGCGACC TTGACCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCCT GCTTGAGTCT 
TGTACGGAGT TTGCCGGAGT GACCCAGCAC CCAGATACTA TAAAGAAGGT CCACCTACAG 
AAGGGGAAGC AGCAGGCCAG GGTCCCCTGG CCCCCGCAGC TCCTGGAGGA GGAGCCTTTC 
CTAAAAGGTC CTGCTCTGGA GCTGGTGGCC ACTGTAGCCA AGGAACCTGG GCCCATTGCA 
CCTTCGACAA ACTCCTCCCC CATCCTGAAA ACCAAGCCCC AACCCCGCCC CGGCTTCCTG 
TCTCACTCTG CTTCATGTGA CTGCTCGATC TGTGCCAGCC CTGTCCTGTC AGCAGTCTGT 
CTGCGCTGGG TGTTGGTCAC AGCAGGCGTG AGGCTGGCCA CAGGCCATCA GGCTCAGGGT 
CTAGAGCTGC TGCAGGTTGT TCTAAAGGGT TGCCCTGCAG CCACCAAGCG CCTCACCCAA 
ACTCTCCAGG CTTCCCTGAA TCACAGAGCC CCTGCCTCTC CTGACCCAAG CCTTCTTGAT 
GAGCTCCTGG CTCAGGCATA CACACAGCTG GCGCTGGCAG GCCTGAAGCA GCCATCAAAC 
AAGAGCCTGC AGAAGATCTT AGAGTCAGGG CTGAAGTTCA CAGCAGCGCG GATCCAACAG 
CTGGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTCAGGCCC TCGTAAAGCT GGCTGGCCTC 
AGCACCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATCAAA 
AGCCCCCCGG GCTCAGAGTC CTCTAAGATC CAGAGCCAAA AACATAGTGG ACGAGGCCAC 
CAGCAAGTAG CCTCTGCTCC CCTGCCCCTC CATAATGCCT CTCACAGAAG CCCAGAAGGT 
GGTGGACCAC CCTGTACACC TAAGCCTGCA GGCCGAGTGA GGCCGGCTGG CCCTCGTGTC 
CCCTTCACTG TGTTTGAAGA TGACTGCCCT ACGAAGAACA AGCCCGAGGT TCCCCAGGCC 
CCCCGGGTAC ATCAGAGGGT CCAGACACGC CTTAAGGTGA ACTTCAGCGA TGACAGTGAC 
TTGGAAGACC CTGTCTCTGC TGAGATACCG CTTGTTGAGG AACCTAAGAG ACGGGGCACT 
GCTTCCTCGG GCCGGGGCCG GGGCCGGGGC CGGGCTAGGA AGAGCCCAAG CTTGGCGATA 
GGTACAGTGG CTGCTCCAGG TAATACCCCA ATGAGTGGTA AGAACCGTAG AACCAAGAAG 
GTGGCATCAA GGCTCTGTGA AGAGCAGGGT CCCCAGACAG CCCCTTGTGG CCAGAACAAG 
CTGGGCCCTG AGCTCATGAG GACTATCCCT GAGGAGAAAC TGACTGACAA GTGCACGGAA 
ATGAGCTTTG AGACCCTTGG AGCCTCTGAC AGAGAAGACT CAGCTTCAGG TGGGAAGGCA 
CTGGTTCCAG GTGCTGATCC CCCCGTAGGT GAATGTGAGG TGTTGAGACG GGATTCCAGC 
AAGGAGGAGC TACCCATCCT GGAACCAGGC AAGGAAAGCA ACAGGGACGT CGGTGCTTGG 
CTCCAACCCC CCGTAACCAT TGGTTCTTCT ACCCTGGATT CCATGCGCGA CTCTCTGAGC 
ATTGCTTTCC GTGGGGTCAG TCATTGCCCT GCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
CTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCCTATGCTA CTGCCTTCCT CGTTGCCGAG 
TCTATCTCTA TTACCTGTCG CCACCAGCTA CTCACTCACC TCCACAGACT GCTCAGCAAG 
GCCCAGAAGC ATCAAGGACC ACTCAGACTG ACAGAGCAGC TGCAGCAGCT AACCCTCCAG 
GAGATGCCTG ATGATGTCCC TCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTCA 
GGAGCTGGTG CCTTTCCCCA GCCCGAGAAG CAGCATTTCC AGGAGCTCTT GGTGCTGCTC 
CCCAAGGGGG TGACTGTCTG CATGTTGGCT GTGTCCACCC TCCAGCCTGG AACTGTGGGC 
AACACTCTCC TACTGACAAG ACTGGAAAAG GACAACCCCC CAGTCACTGT GCAGATCCCC 
ACTGCCCAGG ATAAGTTCCC TCTGAGTTCA GCCCTGAGAG AATTTGACGC TATCCAGAAG 
GAACAGAAAG AGAACAGCAG CTGCACTGAC AAGCGAGCCT GGTGGACAGG CAGACTGGCC 
CTGGACCACA GAATGGAGGA CCTCATCACT TCCCTGGAGA AGCATGTGTT GGGCTGCTGG 
CGAGGGCTGC TGCTGCCCTC CAGTGAAGAA CCTGGCCCTG AGCATGAGGC CACCCGCCTG 
CAGGAGTTGC TGCAGGACTG TGGCTGGCAG TATCCCGACC CGACTCTGTT AAAAGTCTTG 
CTCAGTGGTG CCAGCACCCT CACAACCCAG GATATTCAGG CCCTGGCCTA TGGGCTGTGC 
CCGACCCAGC CAGCCAGAGC CCAGGAACTC CTGACTGAGG CAGCCAGGCG TCTACAGGGG 
CAGTCAGCAC CAAGCAATAA GCATCTTGTC TTGGTGCTGG ACAGGGACCT GCAGAAGCTG 
CCATGGGAAA GCATACCCTG CCTGCGAGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCAC 
TTCCTGCTCA GCTATTTCAT CCTCAAAGAG GCTGGGGCTG CATCAGTGCT GAGCCAGGGG 
GTGGATCCTC GGAGCACATT TTACGTCCTG AACCCTCACA ATAACCTGGC AAGCACAGAG 
GAGCAATTTC GAGCCAAGTT TAGCAGTGAA GCTGGCTGGA GAGGGGTGGT CGGGGAGGTG 
CCAAGCCCAG AACAGATGCA GATGGCCTTG ACAAAGAACG ACTTATATAT CTATGCAGGT 
CACGGGGCTG GTGCCCGCTT CCTTGATGGG CAGGCAGTAC TGCGGCTAAG CTGCCGGGCA 
GTGGCCTTGT TGTTTGGCTG CAGCAGCGCA GCCCTGGCCG TGCATGGAAA CCTGGAGGGG 
GCTGGCATTG TGCTCAAGTA CATCATGGCT GGCTGCCCCC TGTTTCTGGG AAATCTCTGG 
GATGTGACTG ACCGTGACAT TGACCGCTAC ACAGAAGCTC TCCTGCAGGG ATGGCTTGCA 
GCAGGCCCAG GGGCCCCTCT GCTCCACTAT GTCAGCCAGG CCCGCCAGGC TCCCCGACTC 
AAGTCTCTTA TTGGAGCTGC ACCTGTTGCC TATGGCTTAC CTGTCTTCCT TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espl1 ( XM_013159375.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013014829.1 Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X3, mRNA.

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CloneID	ODo37680
Clone ID Related Accession (Same CDS sequence)	XM_013159375.1 , XM_013159375.2
Accession Version	XM_013159375.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	separin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176410.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTCC CCTGTGGCTT TTTCTGTGCT ATGAGGAACT TCAAAGGAGT CAACTTTGGA 
ACTCTGCTTA GAAGCCAGAA GGATGCTGAA GAGTTGCTGT CTGACTTGAA GGAGTTCTTG 
TCCAAGCCTC CAGCAGGTTT TCCCAGTAGC CGATCTGACT CTGAGAGGAG ACAAACTTGT 
GATGCCATCC TGAGGGCTTG CAACCAACAG CTGACGGCCA AGCTGTCTTG CCCTCAACAC 
CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG GCCTGTGATG GCTATGTGAT GTCCACTCCC 
CAGCGCCCTC CACTCTACCT GGAACGCATT CTTTTCATTT TACTACGGAA TGTTGCTGCT 
CAGGGAAGCC CCATGGCCAT GCTCCACCTT GCCCAGCCTC TCCATGCCTG CTTCGTTCAG 
TGCTCTCGCC AAGCTGCCTC ACAGGACTAT GAGGCTGTGG CTTGGGGCAG CTTTTCTCTG 
CTTTGGAAGG GAGCAGAAGC ACTGTTGGAG CAGAGAGATG CCTTCTCAGC GCGGATGAAG 
GCCTTGAGCT TCCTTGTCCT CCTGGAGGAT GAAAGTACCC CGTGTGAGAT TCCTCACTTT 
GCTTCTCCAA CAGCCTGTCG AGCAGTAGCT GCCTATCAGC TGTTTGATCG CAGTGGTTGT 
GGTATAAACA AAGCAGATGC TGATTTCTTA TATGATTTGC TCTCCAAAAA TGTGATCAGA 
ACCTTGGTGG GTGAGGAAAG TGGCTCTCCC AGTCCTCTTT CTTCCCAAAG GGCCCTCTGC 
CTCTTGGAGC TCACCTTAGA ACACTGCCGT CGCCTCTGCT TTAGCCACCA CTATGACAAG 
GCCACCAGTG CAGTTGAGAA GGCTCGTGGT TACCTAAGGG ACACCAGTGT AGCCTCCAGC 
CTCCAGCTAT GCCAGTTGGG GGTTGAGCTG CTACATGTTG GGGTGGAAGG ATCCCAGGCA 
GTGGCCCAGC TTCTCATCAA GGCATCAGCT GTCTTGAATA ACAGTTTGAA GGCCCCATCA 
CCTCCACTTC GGGCATTGTT TGAGACCTCC CAGTTCCTCC TTTCAGGCCT GGAACGAGGC 
ATCAAAAGAC ACTCTGGACC TGATGCTATT GGGAGCCTCT TCACTTTTCT TGGAGGATAC 
TGCTTAATTA TCCAGCAGCT ACGGAATGAT GGTGCCTGTG TGCACTCTCC CAAACAGCAG 
CAGTCTTTGG TGCAGACGCA CTTTCAGGGT CTTCACCTCT ACACTATGAT GGTTTATGAC 
TTTGCCCAGC GCTGTCAGGT AGCTGATTTG GCTGACCTGG CTCAACTAGT GGACAGTTGC 
AGATCTACTG CTGTTTGGAT GCTGGAAGCC TTGGAAGGCC TGTCAGGCCT GGAGCTGACT 
GACTACATGG CCATGACTGC CTCTTACACC AGTAACTTGG CCTATAGCTT CTACAATCAC 
AAGCTGTACG CCGAGGCCTG TGCCGTCTCT GAGCCACTCT GTCAGCACCT GGGCTCTGCT 
AAGCCAGGCA CTTACCATGC GGTGCCTTCT GAGAAGTTGC ACAGGTACTT CCGGCTACAC 
GTAGAGAGTT TGAAGAAACT GGGTAAACAG GCACAGGGCT GTGAGATGGT GACCTTGTGG 
CTGGCGGCTC TACAGCCCTG CAACCCTGAA CACATGGCTG AGCCCGTCTC TTTCTGGGTT 
CGGGTAAAGA TGGATGCGAC CAGGGCTGGA AACAAGGAGC TACAGCTGAA GACTCTGCGA 
GACAGCCTGA GTGACTGGAA CCCAGAGACC CTGACCCTCC TGCTAAGGGA GGAGCTGCAG 
GCCTACAAGG CAGTACGGGC TGACACTGGG CAGGAACGTT TCAATGTCAT CTGTGACTTG 
CTGGAGCTGA GCCCTGAGGA GACACCAGCT GGGGCCTGGG CTCGAGCCAC CTACCTGGTG 
GAACTGGCTC AGGTGCTTTG CTACCACGAC TTTGCCCAGC AGACAGACTG CTCTGCCCTG 
GATGCTATCC AGGAAGCCCT GCAGCTTCTG GAGGCTGTTA AGCCAGAGGC CCAGGCCAAG 
GATCGGCTTC TGGACGATAA AGCACAGGCA CTGTTGTGGT TCTACATCTG CACTTTGGAG 
GCCAAAATGC GGGAAGGTAT TGAGCGGGAT CGGAGAGCCC AGGCTCCTAG TAACCTGGAG 
GAATTTGAAG TTAATGATCT CAACTATGAA GATAAACTCC AGGAAGATTG TTTCCTGTAC 
AGTAACATTG CCTTTAACCT GGCTGCAGAT GCAGCTCACT CTAAAGCCCT GGACCAAGCC 
CTGGCTCTGT GGAAGGAGGT CCTTACAGAG GGGCAGTTCC CAGCTGTGCG ATGTCTCCAG 
CAGACAGCAG CCTCACTGCA GATCCTTGTG GCCCTCTATC AGCTGGTGGC CAAGCCTCTG 
CAGGCCCTGG AGGCTCTCCT GCTCCTGCGG ACCATCTCTG AAAGACTGAA GGATCACGCA 
AAGGCAGCTA GCTCCTCTTG CCACCTCACC CAGGTCCTCC TGAGCCTCGG CTGTCCCACC 
TATGCCCAGT TTTACCTGGA AGAGGCTGAA TCCGCCCTGA TGCATCTCAG TGAGACCACG 
GACACACGAC TGCTGCTTTC TCTGACGTGT CACCTCCTTC GAAGTCAGCT CTGCTGGACT 
CAACAGAAGG TGACTGAGGG TGTCTCTCTG TTGCTGACTG TCCTTCGGGA TCCTGCCCTC 
CAGAAGTCAT CCAAGGCTTG GTATTTGCTT CGAGTCCAGG CTCTGCAACT ACTGTCCATC 
TACCTTAACC TCTCATCAAA CAGCCTCTCA GATGCTCTGC GAGAGCAGCT TTGGGCTCAA 
GGCTGGCAGA CACCTGAGAC AGCACTCATA GATTCTCATA AGCTCCTTCG AAGCATCATC 
CTCCTGCTGA TGGGCAGCGA TGTCCTCTCC CTACAGAAAG CAGCTGTGGA GACACCATTT 
CTGGATTGTG GTGAAAATCT GGTACAAAAA TGGCAGGTTC TGTCAGAGGT TCTGAGCTGC 
TCACAGAAGC TGGTCTGCTC TCTTGGCCAC TTAGGGAGTG TGAGTGAAGC CAAGGCCTTT 
TGCTTGGATG CCCTGAAACT GACGACAAAG CTGCAGCTCC CGGGCCAGTG TGCCCTGTTC 
CTGGTGCTGA AAGGTGAGCT GGAACTGGCC CGCAGCGACC TTGACCTCTG TCAGTCGGAC 
CTGCAGCAGG TTCTGTTCCT GCTTGAGTCT TGTACGGAGT TTGCCGGAGT GACCCAGCAC 
CCAGATACTA TAAAGAAGGT CCACCTACAG AAGGGGAAGC AGCAGGCCAG GGTCCCCTGG 
CCCCCGCAGC TCCTGGAGGA GGAGCCTTTC CTAAAAGGTC CTGCTCTGGA GCTGGTGGCC 
ACTGTAGCCA AGGAACCTGG GCCCATTGCA CCTTCGACAA ACTCCTCCCC CATCCTGAAA 
ACCAAGCCCC AACCCCGCCC CGGCTTCCTG TCTCACTCTG CTTCATGTGA CTGCTCGATC 
TGTGCCAGCC CTGTCCTGTC AGCAGTCTGT CTGCGCTGGG TGTTGGTCAC AGCAGGCGTG 
AGGCTGGCCA CAGGCCATCA GGCTCAGGGT CTAGAGCTGC TGCAGGTTGT TCTAAAGGGT 
TGCCCTGCAG CCACCAAGCG CCTCACCCAA ACTCTCCAGG CTTCCCTGAA TCACAGAGCC 
CCTGCCTCTC CTGACCCAAG CCTTCTTGAT GAGCTCCTGG CTCAGGCATA CACACAGCTG 
GCGCTGGCAG GCCTGAAGCA GCCATCAAAC AAGAGCCTGC AGAAGATCTT AGAGTCAGGG 
CTGAAGTTCA CAGCAGCGCG GATCCAACAG CTGGAGCCCT GGCGAGCCAG CCTGCTCTTG 
ATTCAGGCCC TCGTAAAGCT GGCTGGCCTC AGCACCTGTA CTACCCAACT TTTTGCAAGC 
TCCTGGGGCT GGCAGCCACC ATTAATCAAA AGCCCCCCGG GCTCAGAGTC CTCTAAGATC 
CAGAGCCAAA AACATAGTGG ACGAGGCCAC CAGCAAGTAG CCTCTGCTCC CCTGCCCCTC 
CATAATGCCT CTCACAGAAG CCCAGAAGGT GGTGGACCAC CCTGTACACC TAAGCCTGCA 
GGCCGAGTGA GGCCGGCTGG CCCTCGTGTC CCCTTCACTG TGTTTGAAGA TGACTGCCCT 
ACGAAGAACA AGCCCGAGGT TCCCCAGGCC CCCCGGGTAC ATCAGAGGGT CCAGACACGC 
CTTAAGGTGA ACTTCAGCGA TGACAGTGAC TTGGAAGACC CTGTCTCTGC TGAGATACCG 
CTTGTTGAGG AACCTAAGAG ACGGGGCACT GCTTCCTCGG GCCGGGGCCG GGGCCGGGGC 
CGGGCTAGGA AGAGCCCAAG CTTGGCGATA GGTACAGTGG CTGCTCCAGG TAATACCCCA 
ATGAGTGGTA AGAACCGTAG AACCAAGAAG GTGGCATCAA GGCTCTGTGA AGAGCAGGGT 
CCCCAGACAG CCCCTTGTGG CCAGAACAAG CTGGGCCCTG AGCTCATGAG GACTATCCCT 
GAGGAGAAAC TGACTGACAA GTGCACGGAA ATGAGCTTTG AGACCCTTGG AGCCTCTGAC 
AGAGAAGACT CAGCTTCAGG TGAATGTGAG GTGTTGAGAC GGGATTCCAG CAAGGAGGAG 
CTACCCATCC TGGAACCAGG CAAGGAAAGC AACAGGGACG TCGGTGCTTG GCTCCAACCC 
CCCGTAACCA TTGGTTCTTC TACCCTGGAT TCCATGCGCG ACTCTCTGAG CATTGCTTTC 
CGTGGGGTCA GTCATTGCCC TGCTAGTGGG CTCTATGCCC ACCTCTGCCG CCTCCTGGCC 
TTGTGCCTGG GCCACCGGGA TCCCTATGCT ACTGCCTTCC TCGTTGCCGA GTCTATCTCT 
ATTACCTGTC GCCACCAGCT ACTCACTCAC CTCCACAGAC TGCTCAGCAA GGCCCAGAAG 
CATCAAGGAC CACTCAGACT GACAGAGCAG CTGCAGCAGC TAACCCTCCA GGAGATGCCT 
GATGATGTCC CTCTGGCCCG CATCCAGCGC CTCTTTTCCT TCAGGGCTTC AGGAGCTGGT 
GCCTTTCCCC AGCCCGAGAA GCAGCATTTC CAGGAGCTCT TGGTGCTGCT CCCCAAGGGG 
GTGACTGTCT GCATGTTGGC TGTGTCCACC CTCCAGCCTG GAACTGTGGG CAACACTCTC 
CTACTGACAA GACTGGAAAA GGACAACCCC CCAGTCACTG TGCAGATCCC CACTGCCCAG 
GATAAGTTCC CTCTGAGTTC AGCCCTGAGA GAATTTGACG CTATCCAGAA GGAACAGAAA 
GAGAACAGCA GCTGCACTGA CAAGCGAGCC TGGTGGACAG GCAGACTGGC CCTGGACCAC 
AGAATGGAGG ACCTCATCAC TTCCCTGGAG AAGCATGTGT TGGGCTGCTG GCGAGGGCTG 
CTGCTGCCCT CCAGTGAAGA ACCTGGCCCT GAGCATGAGG CCACCCGCCT GCAGGAGTTG 
CTGCAGGACT GTGGCTGGCA GTATCCCGAC CCGACTCTGT TAAAAGTCTT GCTCAGTGGT 
GCCAGCACCC TCACAACCCA GGATATTCAG GCCCTGGCCT ATGGGCTGTG CCCGACCCAG 
CCAGCCAGAG CCCAGGAACT CCTGACTGAG GCAGCCAGGC GTCTACAGGG GCAGTCAGCA 
CCAAGCAATA AGCATCTTGT CTTGGTGCTG GACAGGGACC TGCAGAAGCT GCCATGGGAA 
AGCATACCCT GCCTGCGAGC ACTGCCTGTC ACCCGGCTGC CCTCCTTCCA CTTCCTGCTC 
AGCTATTTCA TCCTCAAAGA GGCTGGGGCT GCATCAGTGC TGAGCCAGGG GGTGGATCCT 
CGGAGCACAT TTTACGTCCT GAACCCTCAC AATAACCTGG CAAGCACAGA GGAGCAATTT 
CGAGCCAAGT TTAGCAGTGA AGCTGGCTGG AGAGGGGTGG TCGGGGAGGT GCCAAGCCCA 
GAACAGATGC AGATGGCCTT GACAAAGAAC GACTTATATA TCTATGCAGG TCACGGGGCT 
GGTGCCCGCT TCCTTGATGG GCAGGCAGTA CTGCGGCTAA GCTGCCGGGC AGTGGCCTTG 
TTGTTTGGCT GCAGCAGCGC AGCCCTGGCC GTGCATGGAA ACCTGGAGGG GGCTGGCATT 
GTGCTCAAGT ACATCATGGC TGGCTGCCCC CTGTTTCTGG GAAATCTCTG GGATGTGACT 
GACCGTGACA TTGACCGCTA CACAGAAGCT CTCCTGCAGG GATGGCTTGC AGCAGGCCCA 
GGGGCCCCTC TGCTCCACTA TGTCAGCCAG GCCCGCCAGG CTCCCCGACT CAAGTCTCTT 
ATTGGAGCTG CACCTGTTGC CTATGGCTTA CCTGTCTTCC TTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013014829.1
CDS	677..7024
Translation	MCFPCGFFCAMRNFKGVNFGTLLRSQKDAEELLSDLKEFLSKPPAGFPSSRSDSERRQTCDAILRACNQQLTAKLSCPQHLGSLLELAELACDGYVMSTPQRPPLYLERILFILLRNVAAQGSPMAMLHLAQPLHACFVQCSRQAASQDYEAVAWGSFSLLWKGAEALLEQRDAFSARMKALSFLVLLEDESTPCEIPHFASPTACRAVAAYQLFDRSGCGINKADADFLYDLLSKNVIRTLVGEESGSPSPLSSQRALCLLELTLEHCRRLCFSHHYDKATSAVEKARGYLRDTSVASSLQLCQLGVELLHVGVEGSQAVAQLLIKASAVLNNSLKAPSPPLRALFETSQFLLSGLERGIKRHSGPDAIGSLFTFLGGYCLIIQQLRNDGACVHSPKQQQSLVQTHFQGLHLYTMMVYDFAQRCQVADLADLAQLVDSCRSTAVWMLEALEGLSGLELTDYMAMTASYTSNLAYSFYNHKLYAEACAVSEPLCQHLGSAKPGTYHAVPSEKLHRYFRLHVESLKKLGKQAQGCEMVTLWLAALQPCNPEHMAEPVSFWVRVKMDATRAGNKELQLKTLRDSLSDWNPETLTLLLREELQAYKAVRADTGQERFNVICDLLELSPEETPAGAWARATYLVELAQVLCYHDFAQQTDCSALDAIQEALQLLEAVKPEAQAKDRLLDDKAQALLWFYICTLEAKMREGIERDRRAQAPSNLEEFEVNDLNYEDKLQEDCFLYSNIAFNLAADAAHSKALDQALALWKEVLTEGQFPAVRCLQQTAASLQILVALYQLVAKPLQALEALLLLRTISERLKDHAKAASSSCHLTQVLLSLGCPTYAQFYLEEAESALMHLSETTDTRLLLSLTCHLLRSQLCWTQQKVTEGVSLLLTVLRDPALQKSSKAWYLLRVQALQLLSIYLNLSSNSLSDALREQLWAQGWQTPETALIDSHKLLRSIILLLMGSDVLSLQKAAVETPFLDCGENLVQKWQVLSEVLSCSQKLVCSLGHLGSVSEAKAFCLDALKLTTKLQLPGQCALFLVLKGELELARSDLDLCQSDLQQVLFLLESCTEFAGVTQHPDTIKKVHLQKGKQQARVPWPPQLLEEEPFLKGPALELVATVAKEPGPIAPSTNSSPILKTKPQPRPGFLSHSASCDCSICASPVLSAVCLRWVLVTAGVRLATGHQAQGLELLQVVLKGCPAATKRLTQTLQASLNHRAPASPDPSLLDELLAQAYTQLALAGLKQPSNKSLQKILESGLKFTAARIQQLEPWRASLLLIQALVKLAGLSTCTTQLFASSWGWQPPLIKSPPGSESSKIQSQKHSGRGHQQVASAPLPLHNASHRSPEGGGPPCTPKPAGRVRPAGPRVPFTVFEDDCPTKNKPEVPQAPRVHQRVQTRLKVNFSDDSDLEDPVSAEIPLVEEPKRRGTASSGRGRGRGRARKSPSLAIGTVAAPGNTPMSGKNRRTKKVASRLCEEQGPQTAPCGQNKLGPELMRTIPEEKLTDKCTEMSFETLGASDREDSASGECEVLRRDSSKEELPILEPGKESNRDVGAWLQPPVTIGSSTLDSMRDSLSIAFRGVSHCPASGLYAHLCRLLALCLGHRDPYATAFLVAESISITCRHQLLTHLHRLLSKAQKHQGPLRLTEQLQQLTLQEMPDDVPLARIQRLFSFRASGAGAFPQPEKQHFQELLVLLPKGVTVCMLAVSTLQPGTVGNTLLLTRLEKDNPPVTVQIPTAQDKFPLSSALREFDAIQKEQKENSSCTDKRAWWTGRLALDHRMEDLITSLEKHVLGCWRGLLLPSSEEPGPEHEATRLQELLQDCGWQYPDPTLLKVLLSGASTLTTQDIQALAYGLCPTQPARAQELLTEAARRLQGQSAPSNKHLVLVLDRDLQKLPWESIPCLRALPVTRLPSFHFLLSYFILKEAGAASVLSQGVDPRSTFYVLNPHNNLASTEEQFRAKFSSEAGWRGVVGEVPSPEQMQMALTKNDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLAAGPGAPLLHYVSQARQAPRLKSLIGAAPVAYGLPVFLR

Target ORF information:

RefSeq Version	XM_013159375.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013159375.1

ORF Insert Sequence:

ATGTGCTTCC CCTGTGGCTT TTTCTGTGCT ATGAGGAACT TCAAAGGAGT CAACTTTGGA 
ACTCTGCTTA GAAGCCAGAA GGATGCTGAA GAGTTGCTGT CTGACTTGAA GGAGTTCTTG 
TCCAAGCCTC CAGCAGGTTT TCCCAGTAGC CGATCTGACT CTGAGAGGAG ACAAACTTGT 
GATGCCATCC TGAGGGCTTG CAACCAACAG CTGACGGCCA AGCTGTCTTG CCCTCAACAC 
CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG GCCTGTGATG GCTATGTGAT GTCCACTCCC 
CAGCGCCCTC CACTCTACCT GGAACGCATT CTTTTCATTT TACTACGGAA TGTTGCTGCT 
CAGGGAAGCC CCATGGCCAT GCTCCACCTT GCCCAGCCTC TCCATGCCTG CTTCGTTCAG 
TGCTCTCGCC AAGCTGCCTC ACAGGACTAT GAGGCTGTGG CTTGGGGCAG CTTTTCTCTG 
CTTTGGAAGG GAGCAGAAGC ACTGTTGGAG CAGAGAGATG CCTTCTCAGC GCGGATGAAG 
GCCTTGAGCT TCCTTGTCCT CCTGGAGGAT GAAAGTACCC CGTGTGAGAT TCCTCACTTT 
GCTTCTCCAA CAGCCTGTCG AGCAGTAGCT GCCTATCAGC TGTTTGATCG CAGTGGTTGT 
GGTATAAACA AAGCAGATGC TGATTTCTTA TATGATTTGC TCTCCAAAAA TGTGATCAGA 
ACCTTGGTGG GTGAGGAAAG TGGCTCTCCC AGTCCTCTTT CTTCCCAAAG GGCCCTCTGC 
CTCTTGGAGC TCACCTTAGA ACACTGCCGT CGCCTCTGCT TTAGCCACCA CTATGACAAG 
GCCACCAGTG CAGTTGAGAA GGCTCGTGGT TACCTAAGGG ACACCAGTGT AGCCTCCAGC 
CTCCAGCTAT GCCAGTTGGG GGTTGAGCTG CTACATGTTG GGGTGGAAGG ATCCCAGGCA 
GTGGCCCAGC TTCTCATCAA GGCATCAGCT GTCTTGAATA ACAGTTTGAA GGCCCCATCA 
CCTCCACTTC GGGCATTGTT TGAGACCTCC CAGTTCCTCC TTTCAGGCCT GGAACGAGGC 
ATCAAAAGAC ACTCTGGACC TGATGCTATT GGGAGCCTCT TCACTTTTCT TGGAGGATAC 
TGCTTAATTA TCCAGCAGCT ACGGAATGAT GGTGCCTGTG TGCACTCTCC CAAACAGCAG 
CAGTCTTTGG TGCAGACGCA CTTTCAGGGT CTTCACCTCT ACACTATGAT GGTTTATGAC 
TTTGCCCAGC GCTGTCAGGT AGCTGATTTG GCTGACCTGG CTCAACTAGT GGACAGTTGC 
AGATCTACTG CTGTTTGGAT GCTGGAAGCC TTGGAAGGCC TGTCAGGCCT GGAGCTGACT 
GACTACATGG CCATGACTGC CTCTTACACC AGTAACTTGG CCTATAGCTT CTACAATCAC 
AAGCTGTACG CCGAGGCCTG TGCCGTCTCT GAGCCACTCT GTCAGCACCT GGGCTCTGCT 
AAGCCAGGCA CTTACCATGC GGTGCCTTCT GAGAAGTTGC ACAGGTACTT CCGGCTACAC 
GTAGAGAGTT TGAAGAAACT GGGTAAACAG GCACAGGGCT GTGAGATGGT GACCTTGTGG 
CTGGCGGCTC TACAGCCCTG CAACCCTGAA CACATGGCTG AGCCCGTCTC TTTCTGGGTT 
CGGGTAAAGA TGGATGCGAC CAGGGCTGGA AACAAGGAGC TACAGCTGAA GACTCTGCGA 
GACAGCCTGA GTGACTGGAA CCCAGAGACC CTGACCCTCC TGCTAAGGGA GGAGCTGCAG 
GCCTACAAGG CAGTACGGGC TGACACTGGG CAGGAACGTT TCAATGTCAT CTGTGACTTG 
CTGGAGCTGA GCCCTGAGGA GACACCAGCT GGGGCCTGGG CTCGAGCCAC CTACCTGGTG 
GAACTGGCTC AGGTGCTTTG CTACCACGAC TTTGCCCAGC AGACAGACTG CTCTGCCCTG 
GATGCTATCC AGGAAGCCCT GCAGCTTCTG GAGGCTGTTA AGCCAGAGGC CCAGGCCAAG 
GATCGGCTTC TGGACGATAA AGCACAGGCA CTGTTGTGGT TCTACATCTG CACTTTGGAG 
GCCAAAATGC GGGAAGGTAT TGAGCGGGAT CGGAGAGCCC AGGCTCCTAG TAACCTGGAG 
GAATTTGAAG TTAATGATCT CAACTATGAA GATAAACTCC AGGAAGATTG TTTCCTGTAC 
AGTAACATTG CCTTTAACCT GGCTGCAGAT GCAGCTCACT CTAAAGCCCT GGACCAAGCC 
CTGGCTCTGT GGAAGGAGGT CCTTACAGAG GGGCAGTTCC CAGCTGTGCG ATGTCTCCAG 
CAGACAGCAG CCTCACTGCA GATCCTTGTG GCCCTCTATC AGCTGGTGGC CAAGCCTCTG 
CAGGCCCTGG AGGCTCTCCT GCTCCTGCGG ACCATCTCTG AAAGACTGAA GGATCACGCA 
AAGGCAGCTA GCTCCTCTTG CCACCTCACC CAGGTCCTCC TGAGCCTCGG CTGTCCCACC 
TATGCCCAGT TTTACCTGGA AGAGGCTGAA TCCGCCCTGA TGCATCTCAG TGAGACCACG 
GACACACGAC TGCTGCTTTC TCTGACGTGT CACCTCCTTC GAAGTCAGCT CTGCTGGACT 
CAACAGAAGG TGACTGAGGG TGTCTCTCTG TTGCTGACTG TCCTTCGGGA TCCTGCCCTC 
CAGAAGTCAT CCAAGGCTTG GTATTTGCTT CGAGTCCAGG CTCTGCAACT ACTGTCCATC 
TACCTTAACC TCTCATCAAA CAGCCTCTCA GATGCTCTGC GAGAGCAGCT TTGGGCTCAA 
GGCTGGCAGA CACCTGAGAC AGCACTCATA GATTCTCATA AGCTCCTTCG AAGCATCATC 
CTCCTGCTGA TGGGCAGCGA TGTCCTCTCC CTACAGAAAG CAGCTGTGGA GACACCATTT 
CTGGATTGTG GTGAAAATCT GGTACAAAAA TGGCAGGTTC TGTCAGAGGT TCTGAGCTGC 
TCACAGAAGC TGGTCTGCTC TCTTGGCCAC TTAGGGAGTG TGAGTGAAGC CAAGGCCTTT 
TGCTTGGATG CCCTGAAACT GACGACAAAG CTGCAGCTCC CGGGCCAGTG TGCCCTGTTC 
CTGGTGCTGA AAGGTGAGCT GGAACTGGCC CGCAGCGACC TTGACCTCTG TCAGTCGGAC 
CTGCAGCAGG TTCTGTTCCT GCTTGAGTCT TGTACGGAGT TTGCCGGAGT GACCCAGCAC 
CCAGATACTA TAAAGAAGGT CCACCTACAG AAGGGGAAGC AGCAGGCCAG GGTCCCCTGG 
CCCCCGCAGC TCCTGGAGGA GGAGCCTTTC CTAAAAGGTC CTGCTCTGGA GCTGGTGGCC 
ACTGTAGCCA AGGAACCTGG GCCCATTGCA CCTTCGACAA ACTCCTCCCC CATCCTGAAA 
ACCAAGCCCC AACCCCGCCC CGGCTTCCTG TCTCACTCTG CTTCATGTGA CTGCTCGATC 
TGTGCCAGCC CTGTCCTGTC AGCAGTCTGT CTGCGCTGGG TGTTGGTCAC AGCAGGCGTG 
AGGCTGGCCA CAGGCCATCA GGCTCAGGGT CTAGAGCTGC TGCAGGTTGT TCTAAAGGGT 
TGCCCTGCAG CCACCAAGCG CCTCACCCAA ACTCTCCAGG CTTCCCTGAA TCACAGAGCC 
CCTGCCTCTC CTGACCCAAG CCTTCTTGAT GAGCTCCTGG CTCAGGCATA CACACAGCTG 
GCGCTGGCAG GCCTGAAGCA GCCATCAAAC AAGAGCCTGC AGAAGATCTT AGAGTCAGGG 
CTGAAGTTCA CAGCAGCGCG GATCCAACAG CTGGAGCCCT GGCGAGCCAG CCTGCTCTTG 
ATTCAGGCCC TCGTAAAGCT GGCTGGCCTC AGCACCTGTA CTACCCAACT TTTTGCAAGC 
TCCTGGGGCT GGCAGCCACC ATTAATCAAA AGCCCCCCGG GCTCAGAGTC CTCTAAGATC 
CAGAGCCAAA AACATAGTGG ACGAGGCCAC CAGCAAGTAG CCTCTGCTCC CCTGCCCCTC 
CATAATGCCT CTCACAGAAG CCCAGAAGGT GGTGGACCAC CCTGTACACC TAAGCCTGCA 
GGCCGAGTGA GGCCGGCTGG CCCTCGTGTC CCCTTCACTG TGTTTGAAGA TGACTGCCCT 
ACGAAGAACA AGCCCGAGGT TCCCCAGGCC CCCCGGGTAC ATCAGAGGGT CCAGACACGC 
CTTAAGGTGA ACTTCAGCGA TGACAGTGAC TTGGAAGACC CTGTCTCTGC TGAGATACCG 
CTTGTTGAGG AACCTAAGAG ACGGGGCACT GCTTCCTCGG GCCGGGGCCG GGGCCGGGGC 
CGGGCTAGGA AGAGCCCAAG CTTGGCGATA GGTACAGTGG CTGCTCCAGG TAATACCCCA 
ATGAGTGGTA AGAACCGTAG AACCAAGAAG GTGGCATCAA GGCTCTGTGA AGAGCAGGGT 
CCCCAGACAG CCCCTTGTGG CCAGAACAAG CTGGGCCCTG AGCTCATGAG GACTATCCCT 
GAGGAGAAAC TGACTGACAA GTGCACGGAA ATGAGCTTTG AGACCCTTGG AGCCTCTGAC 
AGAGAAGACT CAGCTTCAGG TGAATGTGAG GTGTTGAGAC GGGATTCCAG CAAGGAGGAG 
CTACCCATCC TGGAACCAGG CAAGGAAAGC AACAGGGACG TCGGTGCTTG GCTCCAACCC 
CCCGTAACCA TTGGTTCTTC TACCCTGGAT TCCATGCGCG ACTCTCTGAG CATTGCTTTC 
CGTGGGGTCA GTCATTGCCC TGCTAGTGGG CTCTATGCCC ACCTCTGCCG CCTCCTGGCC 
TTGTGCCTGG GCCACCGGGA TCCCTATGCT ACTGCCTTCC TCGTTGCCGA GTCTATCTCT 
ATTACCTGTC GCCACCAGCT ACTCACTCAC CTCCACAGAC TGCTCAGCAA GGCCCAGAAG 
CATCAAGGAC CACTCAGACT GACAGAGCAG CTGCAGCAGC TAACCCTCCA GGAGATGCCT 
GATGATGTCC CTCTGGCCCG CATCCAGCGC CTCTTTTCCT TCAGGGCTTC AGGAGCTGGT 
GCCTTTCCCC AGCCCGAGAA GCAGCATTTC CAGGAGCTCT TGGTGCTGCT CCCCAAGGGG 
GTGACTGTCT GCATGTTGGC TGTGTCCACC CTCCAGCCTG GAACTGTGGG CAACACTCTC 
CTACTGACAA GACTGGAAAA GGACAACCCC CCAGTCACTG TGCAGATCCC CACTGCCCAG 
GATAAGTTCC CTCTGAGTTC AGCCCTGAGA GAATTTGACG CTATCCAGAA GGAACAGAAA 
GAGAACAGCA GCTGCACTGA CAAGCGAGCC TGGTGGACAG GCAGACTGGC CCTGGACCAC 
AGAATGGAGG ACCTCATCAC TTCCCTGGAG AAGCATGTGT TGGGCTGCTG GCGAGGGCTG 
CTGCTGCCCT CCAGTGAAGA ACCTGGCCCT GAGCATGAGG CCACCCGCCT GCAGGAGTTG 
CTGCAGGACT GTGGCTGGCA GTATCCCGAC CCGACTCTGT TAAAAGTCTT GCTCAGTGGT 
GCCAGCACCC TCACAACCCA GGATATTCAG GCCCTGGCCT ATGGGCTGTG CCCGACCCAG 
CCAGCCAGAG CCCAGGAACT CCTGACTGAG GCAGCCAGGC GTCTACAGGG GCAGTCAGCA 
CCAAGCAATA AGCATCTTGT CTTGGTGCTG GACAGGGACC TGCAGAAGCT GCCATGGGAA 
AGCATACCCT GCCTGCGAGC ACTGCCTGTC ACCCGGCTGC CCTCCTTCCA CTTCCTGCTC 
AGCTATTTCA TCCTCAAAGA GGCTGGGGCT GCATCAGTGC TGAGCCAGGG GGTGGATCCT 
CGGAGCACAT TTTACGTCCT GAACCCTCAC AATAACCTGG CAAGCACAGA GGAGCAATTT 
CGAGCCAAGT TTAGCAGTGA AGCTGGCTGG AGAGGGGTGG TCGGGGAGGT GCCAAGCCCA 
GAACAGATGC AGATGGCCTT GACAAAGAAC GACTTATATA TCTATGCAGG TCACGGGGCT 
GGTGCCCGCT TCCTTGATGG GCAGGCAGTA CTGCGGCTAA GCTGCCGGGC AGTGGCCTTG 
TTGTTTGGCT GCAGCAGCGC AGCCCTGGCC GTGCATGGAA ACCTGGAGGG GGCTGGCATT 
GTGCTCAAGT ACATCATGGC TGGCTGCCCC CTGTTTCTGG GAAATCTCTG GGATGTGACT 
GACCGTGACA TTGACCGCTA CACAGAAGCT CTCCTGCAGG GATGGCTTGC AGCAGGCCCA 
GGGGCCCCTC TGCTCCACTA TGTCAGCCAG GCCCGCCAGG CTCCCCGACT CAAGTCTCTT 
ATTGGAGCTG CACCTGTTGC CTATGGCTTA CCTGTCTTCC TTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espl1 ( XM_013159375.2 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013014829.1 Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X3, mRNA.

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CloneID	ODo37680
Clone ID Related Accession (Same CDS sequence)	XM_013159375.1 , XM_013159375.2
Accession Version	XM_013159375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	separin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176410.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_013159375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTCC CCTGTGGCTT TTTCTGTGCT ATGAGGAACT TCAAAGGAGT CAACTTTGGA 
ACTCTGCTTA GAAGCCAGAA GGATGCTGAA GAGTTGCTGT CTGACTTGAA GGAGTTCTTG 
TCCAAGCCTC CAGCAGGTTT TCCCAGTAGC CGATCTGACT CTGAGAGGAG ACAAACTTGT 
GATGCCATCC TGAGGGCTTG CAACCAACAG CTGACGGCCA AGCTGTCTTG CCCTCAACAC 
CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG GCCTGTGATG GCTATGTGAT GTCCACTCCC 
CAGCGCCCTC CACTCTACCT GGAACGCATT CTTTTCATTT TACTACGGAA TGTTGCTGCT 
CAGGGAAGCC CCATGGCCAT GCTCCACCTT GCCCAGCCTC TCCATGCCTG CTTCGTTCAG 
TGCTCTCGCC AAGCTGCCTC ACAGGACTAT GAGGCTGTGG CTTGGGGCAG CTTTTCTCTG 
CTTTGGAAGG GAGCAGAAGC ACTGTTGGAG CAGAGAGATG CCTTCTCAGC GCGGATGAAG 
GCCTTGAGCT TCCTTGTCCT CCTGGAGGAT GAAAGTACCC CGTGTGAGAT TCCTCACTTT 
GCTTCTCCAA CAGCCTGTCG AGCAGTAGCT GCCTATCAGC TGTTTGATCG CAGTGGTTGT 
GGTATAAACA AAGCAGATGC TGATTTCTTA TATGATTTGC TCTCCAAAAA TGTGATCAGA 
ACCTTGGTGG GTGAGGAAAG TGGCTCTCCC AGTCCTCTTT CTTCCCAAAG GGCCCTCTGC 
CTCTTGGAGC TCACCTTAGA ACACTGCCGT CGCCTCTGCT TTAGCCACCA CTATGACAAG 
GCCACCAGTG CAGTTGAGAA GGCTCGTGGT TACCTAAGGG ACACCAGTGT AGCCTCCAGC 
CTCCAGCTAT GCCAGTTGGG GGTTGAGCTG CTACATGTTG GGGTGGAAGG ATCCCAGGCA 
GTGGCCCAGC TTCTCATCAA GGCATCAGCT GTCTTGAATA ACAGTTTGAA GGCCCCATCA 
CCTCCACTTC GGGCATTGTT TGAGACCTCC CAGTTCCTCC TTTCAGGCCT GGAACGAGGC 
ATCAAAAGAC ACTCTGGACC TGATGCTATT GGGAGCCTCT TCACTTTTCT TGGAGGATAC 
TGCTTAATTA TCCAGCAGCT ACGGAATGAT GGTGCCTGTG TGCACTCTCC CAAACAGCAG 
CAGTCTTTGG TGCAGACGCA CTTTCAGGGT CTTCACCTCT ACACTATGAT GGTTTATGAC 
TTTGCCCAGC GCTGTCAGGT AGCTGATTTG GCTGACCTGG CTCAACTAGT GGACAGTTGC 
AGATCTACTG CTGTTTGGAT GCTGGAAGCC TTGGAAGGCC TGTCAGGCCT GGAGCTGACT 
GACTACATGG CCATGACTGC CTCTTACACC AGTAACTTGG CCTATAGCTT CTACAATCAC 
AAGCTGTACG CCGAGGCCTG TGCCGTCTCT GAGCCACTCT GTCAGCACCT GGGCTCTGCT 
AAGCCAGGCA CTTACCATGC GGTGCCTTCT GAGAAGTTGC ACAGGTACTT CCGGCTACAC 
GTAGAGAGTT TGAAGAAACT GGGTAAACAG GCACAGGGCT GTGAGATGGT GACCTTGTGG 
CTGGCGGCTC TACAGCCCTG CAACCCTGAA CACATGGCTG AGCCCGTCTC TTTCTGGGTT 
CGGGTAAAGA TGGATGCGAC CAGGGCTGGA AACAAGGAGC TACAGCTGAA GACTCTGCGA 
GACAGCCTGA GTGACTGGAA CCCAGAGACC CTGACCCTCC TGCTAAGGGA GGAGCTGCAG 
GCCTACAAGG CAGTACGGGC TGACACTGGG CAGGAACGTT TCAATGTCAT CTGTGACTTG 
CTGGAGCTGA GCCCTGAGGA GACACCAGCT GGGGCCTGGG CTCGAGCCAC CTACCTGGTG 
GAACTGGCTC AGGTGCTTTG CTACCACGAC TTTGCCCAGC AGACAGACTG CTCTGCCCTG 
GATGCTATCC AGGAAGCCCT GCAGCTTCTG GAGGCTGTTA AGCCAGAGGC CCAGGCCAAG 
GATCGGCTTC TGGACGATAA AGCACAGGCA CTGTTGTGGT TCTACATCTG CACTTTGGAG 
GCCAAAATGC GGGAAGGTAT TGAGCGGGAT CGGAGAGCCC AGGCTCCTAG TAACCTGGAG 
GAATTTGAAG TTAATGATCT CAACTATGAA GATAAACTCC AGGAAGATTG TTTCCTGTAC 
AGTAACATTG CCTTTAACCT GGCTGCAGAT GCAGCTCACT CTAAAGCCCT GGACCAAGCC 
CTGGCTCTGT GGAAGGAGGT CCTTACAGAG GGGCAGTTCC CAGCTGTGCG ATGTCTCCAG 
CAGACAGCAG CCTCACTGCA GATCCTTGTG GCCCTCTATC AGCTGGTGGC CAAGCCTCTG 
CAGGCCCTGG AGGCTCTCCT GCTCCTGCGG ACCATCTCTG AAAGACTGAA GGATCACGCA 
AAGGCAGCTA GCTCCTCTTG CCACCTCACC CAGGTCCTCC TGAGCCTCGG CTGTCCCACC 
TATGCCCAGT TTTACCTGGA AGAGGCTGAA TCCGCCCTGA TGCATCTCAG TGAGACCACG 
GACACACGAC TGCTGCTTTC TCTGACGTGT CACCTCCTTC GAAGTCAGCT CTGCTGGACT 
CAACAGAAGG TGACTGAGGG TGTCTCTCTG TTGCTGACTG TCCTTCGGGA TCCTGCCCTC 
CAGAAGTCAT CCAAGGCTTG GTATTTGCTT CGAGTCCAGG CTCTGCAACT ACTGTCCATC 
TACCTTAACC TCTCATCAAA CAGCCTCTCA GATGCTCTGC GAGAGCAGCT TTGGGCTCAA 
GGCTGGCAGA CACCTGAGAC AGCACTCATA GATTCTCATA AGCTCCTTCG AAGCATCATC 
CTCCTGCTGA TGGGCAGCGA TGTCCTCTCC CTACAGAAAG CAGCTGTGGA GACACCATTT 
CTGGATTGTG GTGAAAATCT GGTACAAAAA TGGCAGGTTC TGTCAGAGGT TCTGAGCTGC 
TCACAGAAGC TGGTCTGCTC TCTTGGCCAC TTAGGGAGTG TGAGTGAAGC CAAGGCCTTT 
TGCTTGGATG CCCTGAAACT GACGACAAAG CTGCAGCTCC CGGGCCAGTG TGCCCTGTTC 
CTGGTGCTGA AAGGTGAGCT GGAACTGGCC CGCAGCGACC TTGACCTCTG TCAGTCGGAC 
CTGCAGCAGG TTCTGTTCCT GCTTGAGTCT TGTACGGAGT TTGCCGGAGT GACCCAGCAC 
CCAGATACTA TAAAGAAGGT CCACCTACAG AAGGGGAAGC AGCAGGCCAG GGTCCCCTGG 
CCCCCGCAGC TCCTGGAGGA GGAGCCTTTC CTAAAAGGTC CTGCTCTGGA GCTGGTGGCC 
ACTGTAGCCA AGGAACCTGG GCCCATTGCA CCTTCGACAA ACTCCTCCCC CATCCTGAAA 
ACCAAGCCCC AACCCCGCCC CGGCTTCCTG TCTCACTCTG CTTCATGTGA CTGCTCGATC 
TGTGCCAGCC CTGTCCTGTC AGCAGTCTGT CTGCGCTGGG TGTTGGTCAC AGCAGGCGTG 
AGGCTGGCCA CAGGCCATCA GGCTCAGGGT CTAGAGCTGC TGCAGGTTGT TCTAAAGGGT 
TGCCCTGCAG CCACCAAGCG CCTCACCCAA ACTCTCCAGG CTTCCCTGAA TCACAGAGCC 
CCTGCCTCTC CTGACCCAAG CCTTCTTGAT GAGCTCCTGG CTCAGGCATA CACACAGCTG 
GCGCTGGCAG GCCTGAAGCA GCCATCAAAC AAGAGCCTGC AGAAGATCTT AGAGTCAGGG 
CTGAAGTTCA CAGCAGCGCG GATCCAACAG CTGGAGCCCT GGCGAGCCAG CCTGCTCTTG 
ATTCAGGCCC TCGTAAAGCT GGCTGGCCTC AGCACCTGTA CTACCCAACT TTTTGCAAGC 
TCCTGGGGCT GGCAGCCACC ATTAATCAAA AGCCCCCCGG GCTCAGAGTC CTCTAAGATC 
CAGAGCCAAA AACATAGTGG ACGAGGCCAC CAGCAAGTAG CCTCTGCTCC CCTGCCCCTC 
CATAATGCCT CTCACAGAAG CCCAGAAGGT GGTGGACCAC CCTGTACACC TAAGCCTGCA 
GGCCGAGTGA GGCCGGCTGG CCCTCGTGTC CCCTTCACTG TGTTTGAAGA TGACTGCCCT 
ACGAAGAACA AGCCCGAGGT TCCCCAGGCC CCCCGGGTAC ATCAGAGGGT CCAGACACGC 
CTTAAGGTGA ACTTCAGCGA TGACAGTGAC TTGGAAGACC CTGTCTCTGC TGAGATACCG 
CTTGTTGAGG AACCTAAGAG ACGGGGCACT GCTTCCTCGG GCCGGGGCCG GGGCCGGGGC 
CGGGCTAGGA AGAGCCCAAG CTTGGCGATA GGTACAGTGG CTGCTCCAGG TAATACCCCA 
ATGAGTGGTA AGAACCGTAG AACCAAGAAG GTGGCATCAA GGCTCTGTGA AGAGCAGGGT 
CCCCAGACAG CCCCTTGTGG CCAGAACAAG CTGGGCCCTG AGCTCATGAG GACTATCCCT 
GAGGAGAAAC TGACTGACAA GTGCACGGAA ATGAGCTTTG AGACCCTTGG AGCCTCTGAC 
AGAGAAGACT CAGCTTCAGG TGAATGTGAG GTGTTGAGAC GGGATTCCAG CAAGGAGGAG 
CTACCCATCC TGGAACCAGG CAAGGAAAGC AACAGGGACG TCGGTGCTTG GCTCCAACCC 
CCCGTAACCA TTGGTTCTTC TACCCTGGAT TCCATGCGCG ACTCTCTGAG CATTGCTTTC 
CGTGGGGTCA GTCATTGCCC TGCTAGTGGG CTCTATGCCC ACCTCTGCCG CCTCCTGGCC 
TTGTGCCTGG GCCACCGGGA TCCCTATGCT ACTGCCTTCC TCGTTGCCGA GTCTATCTCT 
ATTACCTGTC GCCACCAGCT ACTCACTCAC CTCCACAGAC TGCTCAGCAA GGCCCAGAAG 
CATCAAGGAC CACTCAGACT GACAGAGCAG CTGCAGCAGC TAACCCTCCA GGAGATGCCT 
GATGATGTCC CTCTGGCCCG CATCCAGCGC CTCTTTTCCT TCAGGGCTTC AGGAGCTGGT 
GCCTTTCCCC AGCCCGAGAA GCAGCATTTC CAGGAGCTCT TGGTGCTGCT CCCCAAGGGG 
GTGACTGTCT GCATGTTGGC TGTGTCCACC CTCCAGCCTG GAACTGTGGG CAACACTCTC 
CTACTGACAA GACTGGAAAA GGACAACCCC CCAGTCACTG TGCAGATCCC CACTGCCCAG 
GATAAGTTCC CTCTGAGTTC AGCCCTGAGA GAATTTGACG CTATCCAGAA GGAACAGAAA 
GAGAACAGCA GCTGCACTGA CAAGCGAGCC TGGTGGACAG GCAGACTGGC CCTGGACCAC 
AGAATGGAGG ACCTCATCAC TTCCCTGGAG AAGCATGTGT TGGGCTGCTG GCGAGGGCTG 
CTGCTGCCCT CCAGTGAAGA ACCTGGCCCT GAGCATGAGG CCACCCGCCT GCAGGAGTTG 
CTGCAGGACT GTGGCTGGCA GTATCCCGAC CCGACTCTGT TAAAAGTCTT GCTCAGTGGT 
GCCAGCACCC TCACAACCCA GGATATTCAG GCCCTGGCCT ATGGGCTGTG CCCGACCCAG 
CCAGCCAGAG CCCAGGAACT CCTGACTGAG GCAGCCAGGC GTCTACAGGG GCAGTCAGCA 
CCAAGCAATA AGCATCTTGT CTTGGTGCTG GACAGGGACC TGCAGAAGCT GCCATGGGAA 
AGCATACCCT GCCTGCGAGC ACTGCCTGTC ACCCGGCTGC CCTCCTTCCA CTTCCTGCTC 
AGCTATTTCA TCCTCAAAGA GGCTGGGGCT GCATCAGTGC TGAGCCAGGG GGTGGATCCT 
CGGAGCACAT TTTACGTCCT GAACCCTCAC AATAACCTGG CAAGCACAGA GGAGCAATTT 
CGAGCCAAGT TTAGCAGTGA AGCTGGCTGG AGAGGGGTGG TCGGGGAGGT GCCAAGCCCA 
GAACAGATGC AGATGGCCTT GACAAAGAAC GACTTATATA TCTATGCAGG TCACGGGGCT 
GGTGCCCGCT TCCTTGATGG GCAGGCAGTA CTGCGGCTAA GCTGCCGGGC AGTGGCCTTG 
TTGTTTGGCT GCAGCAGCGC AGCCCTGGCC GTGCATGGAA ACCTGGAGGG GGCTGGCATT 
GTGCTCAAGT ACATCATGGC TGGCTGCCCC CTGTTTCTGG GAAATCTCTG GGATGTGACT 
GACCGTGACA TTGACCGCTA CACAGAAGCT CTCCTGCAGG GATGGCTTGC AGCAGGCCCA 
GGGGCCCCTC TGCTCCACTA TGTCAGCCAG GCCCGCCAGG CTCCCCGACT CAAGTCTCTT 
ATTGGAGCTG CACCTGTTGC CTATGGCTTA CCTGTCTTCC TTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013014829.1
CDS	679..7026
Translation	MCFPCGFFCAMRNFKGVNFGTLLRSQKDAEELLSDLKEFLSKPPAGFPSSRSDSERRQTCDAILRACNQQLTAKLSCPQHLGSLLELAELACDGYVMSTPQRPPLYLERILFILLRNVAAQGSPMAMLHLAQPLHACFVQCSRQAASQDYEAVAWGSFSLLWKGAEALLEQRDAFSARMKALSFLVLLEDESTPCEIPHFASPTACRAVAAYQLFDRSGCGINKADADFLYDLLSKNVIRTLVGEESGSPSPLSSQRALCLLELTLEHCRRLCFSHHYDKATSAVEKARGYLRDTSVASSLQLCQLGVELLHVGVEGSQAVAQLLIKASAVLNNSLKAPSPPLRALFETSQFLLSGLERGIKRHSGPDAIGSLFTFLGGYCLIIQQLRNDGACVHSPKQQQSLVQTHFQGLHLYTMMVYDFAQRCQVADLADLAQLVDSCRSTAVWMLEALEGLSGLELTDYMAMTASYTSNLAYSFYNHKLYAEACAVSEPLCQHLGSAKPGTYHAVPSEKLHRYFRLHVESLKKLGKQAQGCEMVTLWLAALQPCNPEHMAEPVSFWVRVKMDATRAGNKELQLKTLRDSLSDWNPETLTLLLREELQAYKAVRADTGQERFNVICDLLELSPEETPAGAWARATYLVELAQVLCYHDFAQQTDCSALDAIQEALQLLEAVKPEAQAKDRLLDDKAQALLWFYICTLEAKMREGIERDRRAQAPSNLEEFEVNDLNYEDKLQEDCFLYSNIAFNLAADAAHSKALDQALALWKEVLTEGQFPAVRCLQQTAASLQILVALYQLVAKPLQALEALLLLRTISERLKDHAKAASSSCHLTQVLLSLGCPTYAQFYLEEAESALMHLSETTDTRLLLSLTCHLLRSQLCWTQQKVTEGVSLLLTVLRDPALQKSSKAWYLLRVQALQLLSIYLNLSSNSLSDALREQLWAQGWQTPETALIDSHKLLRSIILLLMGSDVLSLQKAAVETPFLDCGENLVQKWQVLSEVLSCSQKLVCSLGHLGSVSEAKAFCLDALKLTTKLQLPGQCALFLVLKGELELARSDLDLCQSDLQQVLFLLESCTEFAGVTQHPDTIKKVHLQKGKQQARVPWPPQLLEEEPFLKGPALELVATVAKEPGPIAPSTNSSPILKTKPQPRPGFLSHSASCDCSICASPVLSAVCLRWVLVTAGVRLATGHQAQGLELLQVVLKGCPAATKRLTQTLQASLNHRAPASPDPSLLDELLAQAYTQLALAGLKQPSNKSLQKILESGLKFTAARIQQLEPWRASLLLIQALVKLAGLSTCTTQLFASSWGWQPPLIKSPPGSESSKIQSQKHSGRGHQQVASAPLPLHNASHRSPEGGGPPCTPKPAGRVRPAGPRVPFTVFEDDCPTKNKPEVPQAPRVHQRVQTRLKVNFSDDSDLEDPVSAEIPLVEEPKRRGTASSGRGRGRGRARKSPSLAIGTVAAPGNTPMSGKNRRTKKVASRLCEEQGPQTAPCGQNKLGPELMRTIPEEKLTDKCTEMSFETLGASDREDSASGECEVLRRDSSKEELPILEPGKESNRDVGAWLQPPVTIGSSTLDSMRDSLSIAFRGVSHCPASGLYAHLCRLLALCLGHRDPYATAFLVAESISITCRHQLLTHLHRLLSKAQKHQGPLRLTEQLQQLTLQEMPDDVPLARIQRLFSFRASGAGAFPQPEKQHFQELLVLLPKGVTVCMLAVSTLQPGTVGNTLLLTRLEKDNPPVTVQIPTAQDKFPLSSALREFDAIQKEQKENSSCTDKRAWWTGRLALDHRMEDLITSLEKHVLGCWRGLLLPSSEEPGPEHEATRLQELLQDCGWQYPDPTLLKVLLSGASTLTTQDIQALAYGLCPTQPARAQELLTEAARRLQGQSAPSNKHLVLVLDRDLQKLPWESIPCLRALPVTRLPSFHFLLSYFILKEAGAASVLSQGVDPRSTFYVLNPHNNLASTEEQFRAKFSSEAGWRGVVGEVPSPEQMQMALTKNDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLAAGPGAPLLHYVSQARQAPRLKSLIGAAPVAYGLPVFLR

Target ORF information:

RefSeq Version	XM_013159375.2
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus extra spindle pole bodies like 1, separase (Espl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013159375.2

ORF Insert Sequence:

ATGTGCTTCC CCTGTGGCTT TTTCTGTGCT ATGAGGAACT TCAAAGGAGT CAACTTTGGA 
ACTCTGCTTA GAAGCCAGAA GGATGCTGAA GAGTTGCTGT CTGACTTGAA GGAGTTCTTG 
TCCAAGCCTC CAGCAGGTTT TCCCAGTAGC CGATCTGACT CTGAGAGGAG ACAAACTTGT 
GATGCCATCC TGAGGGCTTG CAACCAACAG CTGACGGCCA AGCTGTCTTG CCCTCAACAC 
CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG GCCTGTGATG GCTATGTGAT GTCCACTCCC 
CAGCGCCCTC CACTCTACCT GGAACGCATT CTTTTCATTT TACTACGGAA TGTTGCTGCT 
CAGGGAAGCC CCATGGCCAT GCTCCACCTT GCCCAGCCTC TCCATGCCTG CTTCGTTCAG 
TGCTCTCGCC AAGCTGCCTC ACAGGACTAT GAGGCTGTGG CTTGGGGCAG CTTTTCTCTG 
CTTTGGAAGG GAGCAGAAGC ACTGTTGGAG CAGAGAGATG CCTTCTCAGC GCGGATGAAG 
GCCTTGAGCT TCCTTGTCCT CCTGGAGGAT GAAAGTACCC CGTGTGAGAT TCCTCACTTT 
GCTTCTCCAA CAGCCTGTCG AGCAGTAGCT GCCTATCAGC TGTTTGATCG CAGTGGTTGT 
GGTATAAACA AAGCAGATGC TGATTTCTTA TATGATTTGC TCTCCAAAAA TGTGATCAGA 
ACCTTGGTGG GTGAGGAAAG TGGCTCTCCC AGTCCTCTTT CTTCCCAAAG GGCCCTCTGC 
CTCTTGGAGC TCACCTTAGA ACACTGCCGT CGCCTCTGCT TTAGCCACCA CTATGACAAG 
GCCACCAGTG CAGTTGAGAA GGCTCGTGGT TACCTAAGGG ACACCAGTGT AGCCTCCAGC 
CTCCAGCTAT GCCAGTTGGG GGTTGAGCTG CTACATGTTG GGGTGGAAGG ATCCCAGGCA 
GTGGCCCAGC TTCTCATCAA GGCATCAGCT GTCTTGAATA ACAGTTTGAA GGCCCCATCA 
CCTCCACTTC GGGCATTGTT TGAGACCTCC CAGTTCCTCC TTTCAGGCCT GGAACGAGGC 
ATCAAAAGAC ACTCTGGACC TGATGCTATT GGGAGCCTCT TCACTTTTCT TGGAGGATAC 
TGCTTAATTA TCCAGCAGCT ACGGAATGAT GGTGCCTGTG TGCACTCTCC CAAACAGCAG 
CAGTCTTTGG TGCAGACGCA CTTTCAGGGT CTTCACCTCT ACACTATGAT GGTTTATGAC 
TTTGCCCAGC GCTGTCAGGT AGCTGATTTG GCTGACCTGG CTCAACTAGT GGACAGTTGC 
AGATCTACTG CTGTTTGGAT GCTGGAAGCC TTGGAAGGCC TGTCAGGCCT GGAGCTGACT 
GACTACATGG CCATGACTGC CTCTTACACC AGTAACTTGG CCTATAGCTT CTACAATCAC 
AAGCTGTACG CCGAGGCCTG TGCCGTCTCT GAGCCACTCT GTCAGCACCT GGGCTCTGCT 
AAGCCAGGCA CTTACCATGC GGTGCCTTCT GAGAAGTTGC ACAGGTACTT CCGGCTACAC 
GTAGAGAGTT TGAAGAAACT GGGTAAACAG GCACAGGGCT GTGAGATGGT GACCTTGTGG 
CTGGCGGCTC TACAGCCCTG CAACCCTGAA CACATGGCTG AGCCCGTCTC TTTCTGGGTT 
CGGGTAAAGA TGGATGCGAC CAGGGCTGGA AACAAGGAGC TACAGCTGAA GACTCTGCGA 
GACAGCCTGA GTGACTGGAA CCCAGAGACC CTGACCCTCC TGCTAAGGGA GGAGCTGCAG 
GCCTACAAGG CAGTACGGGC TGACACTGGG CAGGAACGTT TCAATGTCAT CTGTGACTTG 
CTGGAGCTGA GCCCTGAGGA GACACCAGCT GGGGCCTGGG CTCGAGCCAC CTACCTGGTG 
GAACTGGCTC AGGTGCTTTG CTACCACGAC TTTGCCCAGC AGACAGACTG CTCTGCCCTG 
GATGCTATCC AGGAAGCCCT GCAGCTTCTG GAGGCTGTTA AGCCAGAGGC CCAGGCCAAG 
GATCGGCTTC TGGACGATAA AGCACAGGCA CTGTTGTGGT TCTACATCTG CACTTTGGAG 
GCCAAAATGC GGGAAGGTAT TGAGCGGGAT CGGAGAGCCC AGGCTCCTAG TAACCTGGAG 
GAATTTGAAG TTAATGATCT CAACTATGAA GATAAACTCC AGGAAGATTG TTTCCTGTAC 
AGTAACATTG CCTTTAACCT GGCTGCAGAT GCAGCTCACT CTAAAGCCCT GGACCAAGCC 
CTGGCTCTGT GGAAGGAGGT CCTTACAGAG GGGCAGTTCC CAGCTGTGCG ATGTCTCCAG 
CAGACAGCAG CCTCACTGCA GATCCTTGTG GCCCTCTATC AGCTGGTGGC CAAGCCTCTG 
CAGGCCCTGG AGGCTCTCCT GCTCCTGCGG ACCATCTCTG AAAGACTGAA GGATCACGCA 
AAGGCAGCTA GCTCCTCTTG CCACCTCACC CAGGTCCTCC TGAGCCTCGG CTGTCCCACC 
TATGCCCAGT TTTACCTGGA AGAGGCTGAA TCCGCCCTGA TGCATCTCAG TGAGACCACG 
GACACACGAC TGCTGCTTTC TCTGACGTGT CACCTCCTTC GAAGTCAGCT CTGCTGGACT 
CAACAGAAGG TGACTGAGGG TGTCTCTCTG TTGCTGACTG TCCTTCGGGA TCCTGCCCTC 
CAGAAGTCAT CCAAGGCTTG GTATTTGCTT CGAGTCCAGG CTCTGCAACT ACTGTCCATC 
TACCTTAACC TCTCATCAAA CAGCCTCTCA GATGCTCTGC GAGAGCAGCT TTGGGCTCAA 
GGCTGGCAGA CACCTGAGAC AGCACTCATA GATTCTCATA AGCTCCTTCG AAGCATCATC 
CTCCTGCTGA TGGGCAGCGA TGTCCTCTCC CTACAGAAAG CAGCTGTGGA GACACCATTT 
CTGGATTGTG GTGAAAATCT GGTACAAAAA TGGCAGGTTC TGTCAGAGGT TCTGAGCTGC 
TCACAGAAGC TGGTCTGCTC TCTTGGCCAC TTAGGGAGTG TGAGTGAAGC CAAGGCCTTT 
TGCTTGGATG CCCTGAAACT GACGACAAAG CTGCAGCTCC CGGGCCAGTG TGCCCTGTTC 
CTGGTGCTGA AAGGTGAGCT GGAACTGGCC CGCAGCGACC TTGACCTCTG TCAGTCGGAC 
CTGCAGCAGG TTCTGTTCCT GCTTGAGTCT TGTACGGAGT TTGCCGGAGT GACCCAGCAC 
CCAGATACTA TAAAGAAGGT CCACCTACAG AAGGGGAAGC AGCAGGCCAG GGTCCCCTGG 
CCCCCGCAGC TCCTGGAGGA GGAGCCTTTC CTAAAAGGTC CTGCTCTGGA GCTGGTGGCC 
ACTGTAGCCA AGGAACCTGG GCCCATTGCA CCTTCGACAA ACTCCTCCCC CATCCTGAAA 
ACCAAGCCCC AACCCCGCCC CGGCTTCCTG TCTCACTCTG CTTCATGTGA CTGCTCGATC 
TGTGCCAGCC CTGTCCTGTC AGCAGTCTGT CTGCGCTGGG TGTTGGTCAC AGCAGGCGTG 
AGGCTGGCCA CAGGCCATCA GGCTCAGGGT CTAGAGCTGC TGCAGGTTGT TCTAAAGGGT 
TGCCCTGCAG CCACCAAGCG CCTCACCCAA ACTCTCCAGG CTTCCCTGAA TCACAGAGCC 
CCTGCCTCTC CTGACCCAAG CCTTCTTGAT GAGCTCCTGG CTCAGGCATA CACACAGCTG 
GCGCTGGCAG GCCTGAAGCA GCCATCAAAC AAGAGCCTGC AGAAGATCTT AGAGTCAGGG 
CTGAAGTTCA CAGCAGCGCG GATCCAACAG CTGGAGCCCT GGCGAGCCAG CCTGCTCTTG 
ATTCAGGCCC TCGTAAAGCT GGCTGGCCTC AGCACCTGTA CTACCCAACT TTTTGCAAGC 
TCCTGGGGCT GGCAGCCACC ATTAATCAAA AGCCCCCCGG GCTCAGAGTC CTCTAAGATC 
CAGAGCCAAA AACATAGTGG ACGAGGCCAC CAGCAAGTAG CCTCTGCTCC CCTGCCCCTC 
CATAATGCCT CTCACAGAAG CCCAGAAGGT GGTGGACCAC CCTGTACACC TAAGCCTGCA 
GGCCGAGTGA GGCCGGCTGG CCCTCGTGTC CCCTTCACTG TGTTTGAAGA TGACTGCCCT 
ACGAAGAACA AGCCCGAGGT TCCCCAGGCC CCCCGGGTAC ATCAGAGGGT CCAGACACGC 
CTTAAGGTGA ACTTCAGCGA TGACAGTGAC TTGGAAGACC CTGTCTCTGC TGAGATACCG 
CTTGTTGAGG AACCTAAGAG ACGGGGCACT GCTTCCTCGG GCCGGGGCCG GGGCCGGGGC 
CGGGCTAGGA AGAGCCCAAG CTTGGCGATA GGTACAGTGG CTGCTCCAGG TAATACCCCA 
ATGAGTGGTA AGAACCGTAG AACCAAGAAG GTGGCATCAA GGCTCTGTGA AGAGCAGGGT 
CCCCAGACAG CCCCTTGTGG CCAGAACAAG CTGGGCCCTG AGCTCATGAG GACTATCCCT 
GAGGAGAAAC TGACTGACAA GTGCACGGAA ATGAGCTTTG AGACCCTTGG AGCCTCTGAC 
AGAGAAGACT CAGCTTCAGG TGAATGTGAG GTGTTGAGAC GGGATTCCAG CAAGGAGGAG 
CTACCCATCC TGGAACCAGG CAAGGAAAGC AACAGGGACG TCGGTGCTTG GCTCCAACCC 
CCCGTAACCA TTGGTTCTTC TACCCTGGAT TCCATGCGCG ACTCTCTGAG CATTGCTTTC 
CGTGGGGTCA GTCATTGCCC TGCTAGTGGG CTCTATGCCC ACCTCTGCCG CCTCCTGGCC 
TTGTGCCTGG GCCACCGGGA TCCCTATGCT ACTGCCTTCC TCGTTGCCGA GTCTATCTCT 
ATTACCTGTC GCCACCAGCT ACTCACTCAC CTCCACAGAC TGCTCAGCAA GGCCCAGAAG 
CATCAAGGAC CACTCAGACT GACAGAGCAG CTGCAGCAGC TAACCCTCCA GGAGATGCCT 
GATGATGTCC CTCTGGCCCG CATCCAGCGC CTCTTTTCCT TCAGGGCTTC AGGAGCTGGT 
GCCTTTCCCC AGCCCGAGAA GCAGCATTTC CAGGAGCTCT TGGTGCTGCT CCCCAAGGGG 
GTGACTGTCT GCATGTTGGC TGTGTCCACC CTCCAGCCTG GAACTGTGGG CAACACTCTC 
CTACTGACAA GACTGGAAAA GGACAACCCC CCAGTCACTG TGCAGATCCC CACTGCCCAG 
GATAAGTTCC CTCTGAGTTC AGCCCTGAGA GAATTTGACG CTATCCAGAA GGAACAGAAA 
GAGAACAGCA GCTGCACTGA CAAGCGAGCC TGGTGGACAG GCAGACTGGC CCTGGACCAC 
AGAATGGAGG ACCTCATCAC TTCCCTGGAG AAGCATGTGT TGGGCTGCTG GCGAGGGCTG 
CTGCTGCCCT CCAGTGAAGA ACCTGGCCCT GAGCATGAGG CCACCCGCCT GCAGGAGTTG 
CTGCAGGACT GTGGCTGGCA GTATCCCGAC CCGACTCTGT TAAAAGTCTT GCTCAGTGGT 
GCCAGCACCC TCACAACCCA GGATATTCAG GCCCTGGCCT ATGGGCTGTG CCCGACCCAG 
CCAGCCAGAG CCCAGGAACT CCTGACTGAG GCAGCCAGGC GTCTACAGGG GCAGTCAGCA 
CCAAGCAATA AGCATCTTGT CTTGGTGCTG GACAGGGACC TGCAGAAGCT GCCATGGGAA 
AGCATACCCT GCCTGCGAGC ACTGCCTGTC ACCCGGCTGC CCTCCTTCCA CTTCCTGCTC 
AGCTATTTCA TCCTCAAAGA GGCTGGGGCT GCATCAGTGC TGAGCCAGGG GGTGGATCCT 
CGGAGCACAT TTTACGTCCT GAACCCTCAC AATAACCTGG CAAGCACAGA GGAGCAATTT 
CGAGCCAAGT TTAGCAGTGA AGCTGGCTGG AGAGGGGTGG TCGGGGAGGT GCCAAGCCCA 
GAACAGATGC AGATGGCCTT GACAAAGAAC GACTTATATA TCTATGCAGG TCACGGGGCT 
GGTGCCCGCT TCCTTGATGG GCAGGCAGTA CTGCGGCTAA GCTGCCGGGC AGTGGCCTTG 
TTGTTTGGCT GCAGCAGCGC AGCCCTGGCC GTGCATGGAA ACCTGGAGGG GGCTGGCATT 
GTGCTCAAGT ACATCATGGC TGGCTGCCCC CTGTTTCTGG GAAATCTCTG GGATGTGACT 
GACCGTGACA TTGACCGCTA CACAGAAGCT CTCCTGCAGG GATGGCTTGC AGCAGGCCCA 
GGGGCCCCTC TGCTCCACTA TGTCAGCCAG GCCCGCCAGG CTCCCCGACT CAAGTCTCTT 
ATTGGAGCTG CACCTGTTGC CTATGGCTTA CCTGTCTTCC TTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Espl1 cDNA ORF clone, Cavia porcellus(domestic guinea pig)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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