SOS1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	SOS1
Entrez Gene ID	100931009
Full Name	SOS Ras/Rac guanine nucleotide exchange factor 1
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003758336.4	XP_003758384.1	son of sevenless homolog 1 isoform X2
XM_003758337.3	XP_003758385.1	son of sevenless homolog 1 isoform X1
XM_003758336.3	XP_003758384.1	son of sevenless homolog 1 isoform X2
XM_003758337.2	XP_003758385.1	son of sevenless homolog 1 isoform X1
XM_003758337.4	XP_003758385.1	son of sevenless homolog 1 isoform X1
XM_003758336.2	XP_003758384.1	son of sevenless homolog 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following SOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa120072	XM_003758337.4 Latest version!	Sarcophilus harrisii SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa120073	XM_003758336.4 Latest version!	Sarcophilus harrisii SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OSa120073	XM_003758336.3	Sarcophilus harrisii SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa120072	XM_003758337.2	Sarcophilus harrisii son of sevenless homolog 1 (Drosophila) (SOS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa120072	XM_003758337.3	Sarcophilus harrisii SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa120073	XM_003758336.2	Sarcophilus harrisii son of sevenless homolog 1 (Drosophila) (SOS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SOS1 ( XM_003758337.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003758385.1 Sarcophilus harrisii SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa120072
Clone ID Related Accession (Same CDS sequence)	XM_003758337.3 , XM_003758337.2 , XM_003758337.4
Accession Version	XM_003758337.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	son of sevenless homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816756.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_003758337.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC TGCAGCTCCA GTACGAGTTC TTCAGCGAGG AAAACGCGCC CAAGTGGAGG 
GGGCTGCTGG TGACGGCCCT GAAAAAGGTC CAGATGCAAG TCCATCCTAC ACTTGCTTCA 
AATGAGGATG CTCTCCAGTA TGTTGAAGAG TTAATTTTAC AGTTGTTAAG TATGCTGTGC 
CAAGCTCAAC CCCGAAGCGT TTTAGATGTT GAGGATCGTG TACAAAAAAG TTTTCCTCAT 
CCAATTGATA AGTGGGCGAT AGCTGATGCT CAATCCGCTA ATGAAAAGAG GAAGCGAAGA 
AATCCATTAT CTCTCCCAGT AGAAAAAATT CACCCTTTAT TAAAGGAAGT TCTAGGATAT 
AAAATTGACC ACCAGGTTTC TGTTTACATA GTGGCAGTAT TAGAATACAT TTCTGCAGAT 
ATTTTAAAGC TGGTTGGAAA TTATGTACGG AATATACGAC ATTATGAAAT TACAAAACAG 
GACATCAAAG TAGCAATGTG TGCTGATAAG GTATTGATGG ATATGTTTCA TCAAGATGTA 
GAAGATATAA ATATATTATC TTTAACTGAT GAAGAACCTT CCACTTCAGG GGAACAAACG 
TACTATGACT TGGTAAAAGG ATTCATGGCT GAAGTTCGAC AATATATAAG GGAACTTAAT 
CTCATTATTA AAGTTTTTAG AGAACCATTT GTCTCCAATT CAAAATTATT TTCTTCTCAC 
GATGTAGAAA ATATATTTAG TCGTATAGCA GATGTTCATG AACTCAGTGT AAAATTATTG 
GGCCATATAG AAGACACTGT AGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTTGGT 
AGCTGCTTTG AAGACTTAGC AGAGGAATTG GCATTTGATC CATATGAATC ATATGCTCAA 
GACATATTGC GACCTGGTTT TCATGATCAC TTTCTTAGTC AGTTATCCAA GCCTGGGGCA 
GCCCTCTACT TGCAGTCAAT AGGTGAAGGT TTCAAAGAAG CTGTTCAGTA TGTTTTACCC 
AGGCTACTTC TAGCTCCTGT GTACCACTGT CTACATTACT TTGAACTTTT AAAGCAGTTA 
GAAGAGAAGA GTGAAGACCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACGGCTTTG 
CTTAACCTTC AGAGCAGTAT GGAAAGAATA TGTTCCAAAA GTCTTGCAAA ACGGAGATTG 
AGCGAATCTG CATGTCGATT TTATAGCCAA CAGATGAAGG GAAAACAACT AGCAATAAAG 
AAAATGAATG AGATTCAGAA GAACATTGAT GGGTGGGAGG GAAAAGACAT TGGACAGTGT 
TGCAATGAGT TTATCATGGA AGGAACTCTC ACACGTGTAG GTGCCAAACA TGAGAGACAC 
ATATTTCTCT TTGATGGTCT GATGATTTGC TGTAAATCAA ATCATGGTCA GCCAAGACTT 
CCTGGTGCTA GCAATGCTGA GTATCGTCTC AAAGAGAAGT TTTTTATGCG CAAGGTACAA 
ATCAATGATA AGGATGACAC TAATGAATAC AAACATGCCT TTGAAATAAT CTTAAAAGAT 
GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAGGAGA AAAACAACTG GATGGCAGCA 
CTGATATCAT TACAATATCG TAGTACTCTG GAAAGGATGC TGGATGTAAC AATGTTACAA 
GAGGAGAAGG AAGAGCAGAT GAGGCTTCCA AGTTCAGATC TTTATAGATT TGCAGAACCA 
GATTCTGAAG AAAACATAGT ATTTGAAGAA AACATGCAAC CCAAATCTGG AATTCCAATC 
ATCAAAGCAG GAACCGTTAT CAAACTTATA GAGCGACTCA CCTACCATAT GTATGCTGAT 
CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCTT TCTGCAAGCC TCAAGAGCTA 
CTGAGTCTTC TAATAGAGAG GTTTGAAATT CCAGAGCCAG AGCCAACAGA AGCTGATAAG 
ATAGCTATGG AGAATGGAGA TCAACCCCTG AGTGTGGAAC TAAAACGATT TAGAAAAGAA 
TATATACAAC CCGTACAACT TCGAGTACTA AATGTATGTC GGCATTGGGT AGAACATCAC 
TTCTATGATT TTGAAAGGGA TACAGATCTT TTGCAACGAC TGGAAGAATT TATTGGAACA 
GTAAGGGGCA AAGCAATGAA GAAATGGGTT GAATCTATCA CTAAAATCAT TCAACGGAAA 
AAAATGGCAA GAGACAATGG ACCAGGACAT AATATTACAT TTGAAAGTTC ACCACCTGCA 
GTTGAATGGC ATATAAGCAG ACCTGGACAC ACAGAAACTT TTGATCTGCT CACCTTGCAC 
CCAATAGAAA TTGCTCGACA GCTCACTTTA CTTGAGTCAG ATCTCTACAG AGCTGTACAG 
CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAC 
CTTCTGAAAA TGATTCGCCA CACAACTAAT CTTACTTTGT GGTTTGAAAA GTGTATTGTA 
GAAGCAGAAA ACCTAGAGGA AAGAGTAGCT GTGGTGAGCC GAATAATTGA GATTCTTCAA 
GTCTTTCAAG AACTGAACAA CTTTAATGGT GTACTTGAGG TTGTCAGTGC CATGAATTCA 
TCACCTGTTT ATAGACTGGA CCACACGTTT GAGCAAATTC CAAGTCGCCA AAAGAAAATT 
CTAGAGGAAG CTCATGAACT GAGTGAAGAT CACTATAAAA AATATTTGGC AAAACTCAGG 
TCTATTAATC CACCATGTGT GCCTTTCTTT GGGATTTATT TGACAAATAT CTTGAAAACG 
GAAGAAGGCA ATCCTGAGGT TTTGAAAAGA CATGGAAAAG AACTCATAAA CTTTAGCAAA 
AGAAGAAAAG TTGCAGAAAT AACAGGAGAG ATACAGCAGT ACCAAAATCA ACCATATTGT 
TTGCGAGTAG AATCTGATAT CAAAAGATTC TTTGAAAATT TGAATCCAAT GGGAAATAGC 
ATGGAAAAAG AATTCACAGA TTATCTTTTC AACAAGTCAC TGGAAATTGA ACCAAGAAAC 
CCTAAGCCTC TACCGAGATT TCCAAAAAAA TATAGTTATC CCCTCAAGTC TCCTGGTGTT 
CGTCCATCAA ACCCAAGACC AGGTACCATG AGACACCCCA CACCACTGCA GCAGGAACCA 
AGGAAGATCA GTTACAGCCG CATCCCTGAA AGTGAAACAG AAAGTGCAGC ATCAGCACCT 
AATTCTCCAC GGACCCCGTT AACACCTCCT CCAGCTTCTG GTGCTTCCAG TACCACTGAT 
GTGGGCAGCG TGTTTGATTC TGAGCATTCA AGCCCTTTTC ACTCAAGCAG CGATACCATC 
TTTATCCAAG TTACACTGCC CCATGGCCCA AGATCTGCTT CAGTATCCTC AATAAATTTA 
ACTAAGAGCA TTGATGAAAT GCCCATCCCC CCACCAGTGC CCCCAAGAAG ACGACCAGAA 
TCCGCTCCAG CAGAATCTTC TCCCTCAAAG ATTATGCCCA AACATTTGGA TAGTCCTCCA 
GCAATTCCTC CTCGGCAACC CACATCAAAA GTCTATTCAC CACGTTACTC CGACCGGACC 
TCGATCTCTG ATCCTCCAGA AAGCCCTCCC CTTTTACCAC CACGAGAACC TGTGAGGACA 
CCTGATGTTT TCTCCAGCTC ACCACTACAT CTCCAGCCAC CCCCTTTGGG CAAAAAAAGT 
GAACATGGCA ATACTTTTTT CCCAAACAGC CCCTCCCCCT TTACCCCACC ACCTCCTCAA 
ACACCTTCTC CTCACGGCAC AAGGAGGCAC CTACCATCAC CACCATTAAC ACAAGATGTG 
GACCTTCATT CCATCCCTGC GCCTCCTGTT CCTCCACGGC AAAGCACTTC TCAACACATC 
CCAAAACTTC CTCCAAAAAC TTATAAAAGG GAACACACAC ACCCTTCCAT GCATAGAGAT 
GGACCACCTT TGTTGGAGAA TGCCCATTCC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003758385.1
CDS	31..4026
Translation	MQALQLQYEFFSEENAPKWRGLLVTALKKVQMQVHPTLASNEDALQYVEELILQLLSMLCQAQPRSVLDVEDRVQKSFPHPIDKWAIADAQSANEKRKRRNPLSLPVEKIHPLLKEVLGYKIDHQVSVYIVAVLEYISADILKLVGNYVRNIRHYEITKQDIKVAMCADKVLMDMFHQDVEDINILSLTDEEPSTSGEQTYYDLVKGFMAEVRQYIRELNLIIKVFREPFVSNSKLFSSHDVENIFSRIADVHELSVKLLGHIEDTVEMTDEGSPHPLVGSCFEDLAEELAFDPYESYAQDILRPGFHDHFLSQLSKPGAALYLQSIGEGFKEAVQYVLPRLLLAPVYHCLHYFELLKQLEEKSEDQEDKECLKQAITALLNLQSSMERICSKSLAKRRLSESACRFYSQQMKGKQLAIKKMNEIQKNIDGWEGKDIGQCCNEFIMEGTLTRVGAKHERHIFLFDGLMICCKSNHGQPRLPGASNAEYRLKEKFFMRKVQINDKDDTNEYKHAFEIILKDENSVIFSAKSAEEKNNWMAALISLQYRSTLERMLDVTMLQEEKEEQMRLPSSDLYRFAEPDSEENIVFEENMQPKSGIPIIKAGTVIKLIERLTYHMYADPNFVRTFLTTYRSFCKPQELLSLLIERFEIPEPEPTEADKIAMENGDQPLSVELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDTDLLQRLEEFIGTVRGKAMKKWVESITKIIQRKKMARDNGPGHNITFESSPPAVEWHISRPGHTETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIVEAENLEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHELSEDHYKKYLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLKRHGKELINFSKRRKVAEITGEIQQYQNQPYCLRVESDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRNPKPLPRFPKKYSYPLKSPGVRPSNPRPGTMRHPTPLQQEPRKISYSRIPESETESAASAPNSPRTPLTPPPASGASSTTDVGSVFDSEHSSPFHSSSDTIFIQVTLPHGPRSASVSSINLTKSIDEMPIPPPVPPRRRPESAPAESSPSKIMPKHLDSPPAIPPRQPTSKVYSPRYSDRTSISDPPESPPLLPPREPVRTPDVFSSSPLHLQPPPLGKKSEHGNTFFPNSPSPFTPPPPQTPSPHGTRRHLPSPPLTQDVDLHSIPAPPVPPRQSTSQHIPKLPPKTYKREHTHPSMHRDGPPLLENAHSS

Target ORF information:

RefSeq Version	XM_003758337.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003758337.3

ORF Insert Sequence:

ATGCAGGCGC TGCAGCTCCA GTACGAGTTC TTCAGCGAGG AAAACGCGCC CAAGTGGAGG 
GGGCTGCTGG TGACGGCCCT GAAAAAGGTC CAGATGCAAG TCCATCCTAC ACTTGCTTCA 
AATGAGGATG CTCTCCAGTA TGTTGAAGAG TTAATTTTAC AGTTGTTAAG TATGCTGTGC 
CAAGCTCAAC CCCGAAGCGT TTTAGATGTT GAGGATCGTG TACAAAAAAG TTTTCCTCAT 
CCAATTGATA AGTGGGCGAT AGCTGATGCT CAATCCGCTA ATGAAAAGAG GAAGCGAAGA 
AATCCATTAT CTCTCCCAGT AGAAAAAATT CACCCTTTAT TAAAGGAAGT TCTAGGATAT 
AAAATTGACC ACCAGGTTTC TGTTTACATA GTGGCAGTAT TAGAATACAT TTCTGCAGAT 
ATTTTAAAGC TGGTTGGAAA TTATGTACGG AATATACGAC ATTATGAAAT TACAAAACAG 
GACATCAAAG TAGCAATGTG TGCTGATAAG GTATTGATGG ATATGTTTCA TCAAGATGTA 
GAAGATATAA ATATATTATC TTTAACTGAT GAAGAACCTT CCACTTCAGG GGAACAAACG 
TACTATGACT TGGTAAAAGG ATTCATGGCT GAAGTTCGAC AATATATAAG GGAACTTAAT 
CTCATTATTA AAGTTTTTAG AGAACCATTT GTCTCCAATT CAAAATTATT TTCTTCTCAC 
GATGTAGAAA ATATATTTAG TCGTATAGCA GATGTTCATG AACTCAGTGT AAAATTATTG 
GGCCATATAG AAGACACTGT AGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTTGGT 
AGCTGCTTTG AAGACTTAGC AGAGGAATTG GCATTTGATC CATATGAATC ATATGCTCAA 
GACATATTGC GACCTGGTTT TCATGATCAC TTTCTTAGTC AGTTATCCAA GCCTGGGGCA 
GCCCTCTACT TGCAGTCAAT AGGTGAAGGT TTCAAAGAAG CTGTTCAGTA TGTTTTACCC 
AGGCTACTTC TAGCTCCTGT GTACCACTGT CTACATTACT TTGAACTTTT AAAGCAGTTA 
GAAGAGAAGA GTGAAGACCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACGGCTTTG 
CTTAACCTTC AGAGCAGTAT GGAAAGAATA TGTTCCAAAA GTCTTGCAAA ACGGAGATTG 
AGCGAATCTG CATGTCGATT TTATAGCCAA CAGATGAAGG GAAAACAACT AGCAATAAAG 
AAAATGAATG AGATTCAGAA GAACATTGAT GGGTGGGAGG GAAAAGACAT TGGACAGTGT 
TGCAATGAGT TTATCATGGA AGGAACTCTC ACACGTGTAG GTGCCAAACA TGAGAGACAC 
ATATTTCTCT TTGATGGTCT GATGATTTGC TGTAAATCAA ATCATGGTCA GCCAAGACTT 
CCTGGTGCTA GCAATGCTGA GTATCGTCTC AAAGAGAAGT TTTTTATGCG CAAGGTACAA 
ATCAATGATA AGGATGACAC TAATGAATAC AAACATGCCT TTGAAATAAT CTTAAAAGAT 
GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAGGAGA AAAACAACTG GATGGCAGCA 
CTGATATCAT TACAATATCG TAGTACTCTG GAAAGGATGC TGGATGTAAC AATGTTACAA 
GAGGAGAAGG AAGAGCAGAT GAGGCTTCCA AGTTCAGATC TTTATAGATT TGCAGAACCA 
GATTCTGAAG AAAACATAGT ATTTGAAGAA AACATGCAAC CCAAATCTGG AATTCCAATC 
ATCAAAGCAG GAACCGTTAT CAAACTTATA GAGCGACTCA CCTACCATAT GTATGCTGAT 
CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCTT TCTGCAAGCC TCAAGAGCTA 
CTGAGTCTTC TAATAGAGAG GTTTGAAATT CCAGAGCCAG AGCCAACAGA AGCTGATAAG 
ATAGCTATGG AGAATGGAGA TCAACCCCTG AGTGTGGAAC TAAAACGATT TAGAAAAGAA 
TATATACAAC CCGTACAACT TCGAGTACTA AATGTATGTC GGCATTGGGT AGAACATCAC 
TTCTATGATT TTGAAAGGGA TACAGATCTT TTGCAACGAC TGGAAGAATT TATTGGAACA 
GTAAGGGGCA AAGCAATGAA GAAATGGGTT GAATCTATCA CTAAAATCAT TCAACGGAAA 
AAAATGGCAA GAGACAATGG ACCAGGACAT AATATTACAT TTGAAAGTTC ACCACCTGCA 
GTTGAATGGC ATATAAGCAG ACCTGGACAC ACAGAAACTT TTGATCTGCT CACCTTGCAC 
CCAATAGAAA TTGCTCGACA GCTCACTTTA CTTGAGTCAG ATCTCTACAG AGCTGTACAG 
CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAC 
CTTCTGAAAA TGATTCGCCA CACAACTAAT CTTACTTTGT GGTTTGAAAA GTGTATTGTA 
GAAGCAGAAA ACCTAGAGGA AAGAGTAGCT GTGGTGAGCC GAATAATTGA GATTCTTCAA 
GTCTTTCAAG AACTGAACAA CTTTAATGGT GTACTTGAGG TTGTCAGTGC CATGAATTCA 
TCACCTGTTT ATAGACTGGA CCACACGTTT GAGCAAATTC CAAGTCGCCA AAAGAAAATT 
CTAGAGGAAG CTCATGAACT GAGTGAAGAT CACTATAAAA AATATTTGGC AAAACTCAGG 
TCTATTAATC CACCATGTGT GCCTTTCTTT GGGATTTATT TGACAAATAT CTTGAAAACG 
GAAGAAGGCA ATCCTGAGGT TTTGAAAAGA CATGGAAAAG AACTCATAAA CTTTAGCAAA 
AGAAGAAAAG TTGCAGAAAT AACAGGAGAG ATACAGCAGT ACCAAAATCA ACCATATTGT 
TTGCGAGTAG AATCTGATAT CAAAAGATTC TTTGAAAATT TGAATCCAAT GGGAAATAGC 
ATGGAAAAAG AATTCACAGA TTATCTTTTC AACAAGTCAC TGGAAATTGA ACCAAGAAAC 
CCTAAGCCTC TACCGAGATT TCCAAAAAAA TATAGTTATC CCCTCAAGTC TCCTGGTGTT 
CGTCCATCAA ACCCAAGACC AGGTACCATG AGACACCCCA CACCACTGCA GCAGGAACCA 
AGGAAGATCA GTTACAGCCG CATCCCTGAA AGTGAAACAG AAAGTGCAGC ATCAGCACCT 
AATTCTCCAC GGACCCCGTT AACACCTCCT CCAGCTTCTG GTGCTTCCAG TACCACTGAT 
GTGGGCAGCG TGTTTGATTC TGAGCATTCA AGCCCTTTTC ACTCAAGCAG CGATACCATC 
TTTATCCAAG TTACACTGCC CCATGGCCCA AGATCTGCTT CAGTATCCTC AATAAATTTA 
ACTAAGAGCA TTGATGAAAT GCCCATCCCC CCACCAGTGC CCCCAAGAAG ACGACCAGAA 
TCCGCTCCAG CAGAATCTTC TCCCTCAAAG ATTATGCCCA AACATTTGGA TAGTCCTCCA 
GCAATTCCTC CTCGGCAACC CACATCAAAA GTCTATTCAC CACGTTACTC CGACCGGACC 
TCGATCTCTG ATCCTCCAGA AAGCCCTCCC CTTTTACCAC CACGAGAACC TGTGAGGACA 
CCTGATGTTT TCTCCAGCTC ACCACTACAT CTCCAGCCAC CCCCTTTGGG CAAAAAAAGT 
GAACATGGCA ATACTTTTTT CCCAAACAGC CCCTCCCCCT TTACCCCACC ACCTCCTCAA 
ACACCTTCTC CTCACGGCAC AAGGAGGCAC CTACCATCAC CACCATTAAC ACAAGATGTG 
GACCTTCATT CCATCCCTGC GCCTCCTGTT CCTCCACGGC AAAGCACTTC TCAACACATC 
CCAAAACTTC CTCCAAAAAC TTATAAAAGG GAACACACAC ACCCTTCCAT GCATAGAGAT 
GGACCACCTT TGTTGGAGAA TGCCCATTCC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SOS1 ( XM_003758337.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003758385.1 Sarcophilus harrisii son of sevenless homolog 1 (Drosophila) (SOS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa120072
Clone ID Related Accession (Same CDS sequence)	XM_003758337.3 , XM_003758337.2 , XM_003758337.4
Accession Version	XM_003758337.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	son of sevenless homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816756.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_003758337.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC TGCAGCTCCA GTACGAGTTC TTCAGCGAGG AAAACGCGCC CAAGTGGAGG 
GGGCTGCTGG TGACGGCCCT GAAAAAGGTC CAGATGCAAG TCCATCCTAC ACTTGCTTCA 
AATGAGGATG CTCTCCAGTA TGTTGAAGAG TTAATTTTAC AGTTGTTAAG TATGCTGTGC 
CAAGCTCAAC CCCGAAGCGT TTTAGATGTT GAGGATCGTG TACAAAAAAG TTTTCCTCAT 
CCAATTGATA AGTGGGCGAT AGCTGATGCT CAATCCGCTA ATGAAAAGAG GAAGCGAAGA 
AATCCATTAT CTCTCCCAGT AGAAAAAATT CACCCTTTAT TAAAGGAAGT TCTAGGATAT 
AAAATTGACC ACCAGGTTTC TGTTTACATA GTGGCAGTAT TAGAATACAT TTCTGCAGAT 
ATTTTAAAGC TGGTTGGAAA TTATGTACGG AATATACGAC ATTATGAAAT TACAAAACAG 
GACATCAAAG TAGCAATGTG TGCTGATAAG GTATTGATGG ATATGTTTCA TCAAGATGTA 
GAAGATATAA ATATATTATC TTTAACTGAT GAAGAACCTT CCACTTCAGG GGAACAAACG 
TACTATGACT TGGTAAAAGG ATTCATGGCT GAAGTTCGAC AATATATAAG GGAACTTAAT 
CTCATTATTA AAGTTTTTAG AGAACCATTT GTCTCCAATT CAAAATTATT TTCTTCTCAC 
GATGTAGAAA ATATATTTAG TCGTATAGCA GATGTTCATG AACTCAGTGT AAAATTATTG 
GGCCATATAG AAGACACTGT AGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTTGGT 
AGCTGCTTTG AAGACTTAGC AGAGGAATTG GCATTTGATC CATATGAATC ATATGCTCAA 
GACATATTGC GACCTGGTTT TCATGATCAC TTTCTTAGTC AGTTATCCAA GCCTGGGGCA 
GCCCTCTACT TGCAGTCAAT AGGTGAAGGT TTCAAAGAAG CTGTTCAGTA TGTTTTACCC 
AGGCTACTTC TAGCTCCTGT GTACCACTGT CTACATTACT TTGAACTTTT AAAGCAGTTA 
GAAGAGAAGA GTGAAGACCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACGGCTTTG 
CTTAACCTTC AGAGCAGTAT GGAAAGAATA TGTTCCAAAA GTCTTGCAAA ACGGAGATTG 
AGCGAATCTG CATGTCGATT TTATAGCCAA CAGATGAAGG GAAAACAACT AGCAATAAAG 
AAAATGAATG AGATTCAGAA GAACATTGAT GGGTGGGAGG GAAAAGACAT TGGACAGTGT 
TGCAATGAGT TTATCATGGA AGGAACTCTC ACACGTGTAG GTGCCAAACA TGAGAGACAC 
ATATTTCTCT TTGATGGTCT GATGATTTGC TGTAAATCAA ATCATGGTCA GCCAAGACTT 
CCTGGTGCTA GCAATGCTGA GTATCGTCTC AAAGAGAAGT TTTTTATGCG CAAGGTACAA 
ATCAATGATA AGGATGACAC TAATGAATAC AAACATGCCT TTGAAATAAT CTTAAAAGAT 
GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAGGAGA AAAACAACTG GATGGCAGCA 
CTGATATCAT TACAATATCG TAGTACTCTG GAAAGGATGC TGGATGTAAC AATGTTACAA 
GAGGAGAAGG AAGAGCAGAT GAGGCTTCCA AGTTCAGATC TTTATAGATT TGCAGAACCA 
GATTCTGAAG AAAACATAGT ATTTGAAGAA AACATGCAAC CCAAATCTGG AATTCCAATC 
ATCAAAGCAG GAACCGTTAT CAAACTTATA GAGCGACTCA CCTACCATAT GTATGCTGAT 
CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCTT TCTGCAAGCC TCAAGAGCTA 
CTGAGTCTTC TAATAGAGAG GTTTGAAATT CCAGAGCCAG AGCCAACAGA AGCTGATAAG 
ATAGCTATGG AGAATGGAGA TCAACCCCTG AGTGTGGAAC TAAAACGATT TAGAAAAGAA 
TATATACAAC CCGTACAACT TCGAGTACTA AATGTATGTC GGCATTGGGT AGAACATCAC 
TTCTATGATT TTGAAAGGGA TACAGATCTT TTGCAACGAC TGGAAGAATT TATTGGAACA 
GTAAGGGGCA AAGCAATGAA GAAATGGGTT GAATCTATCA CTAAAATCAT TCAACGGAAA 
AAAATGGCAA GAGACAATGG ACCAGGACAT AATATTACAT TTGAAAGTTC ACCACCTGCA 
GTTGAATGGC ATATAAGCAG ACCTGGACAC ACAGAAACTT TTGATCTGCT CACCTTGCAC 
CCAATAGAAA TTGCTCGACA GCTCACTTTA CTTGAGTCAG ATCTCTACAG AGCTGTACAG 
CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAC 
CTTCTGAAAA TGATTCGCCA CACAACTAAT CTTACTTTGT GGTTTGAAAA GTGTATTGTA 
GAAGCAGAAA ACCTAGAGGA AAGAGTAGCT GTGGTGAGCC GAATAATTGA GATTCTTCAA 
GTCTTTCAAG AACTGAACAA CTTTAATGGT GTACTTGAGG TTGTCAGTGC CATGAATTCA 
TCACCTGTTT ATAGACTGGA CCACACGTTT GAGCAAATTC CAAGTCGCCA AAAGAAAATT 
CTAGAGGAAG CTCATGAACT GAGTGAAGAT CACTATAAAA AATATTTGGC AAAACTCAGG 
TCTATTAATC CACCATGTGT GCCTTTCTTT GGGATTTATT TGACAAATAT CTTGAAAACG 
GAAGAAGGCA ATCCTGAGGT TTTGAAAAGA CATGGAAAAG AACTCATAAA CTTTAGCAAA 
AGAAGAAAAG TTGCAGAAAT AACAGGAGAG ATACAGCAGT ACCAAAATCA ACCATATTGT 
TTGCGAGTAG AATCTGATAT CAAAAGATTC TTTGAAAATT TGAATCCAAT GGGAAATAGC 
ATGGAAAAAG AATTCACAGA TTATCTTTTC AACAAGTCAC TGGAAATTGA ACCAAGAAAC 
CCTAAGCCTC TACCGAGATT TCCAAAAAAA TATAGTTATC CCCTCAAGTC TCCTGGTGTT 
CGTCCATCAA ACCCAAGACC AGGTACCATG AGACACCCCA CACCACTGCA GCAGGAACCA 
AGGAAGATCA GTTACAGCCG CATCCCTGAA AGTGAAACAG AAAGTGCAGC ATCAGCACCT 
AATTCTCCAC GGACCCCGTT AACACCTCCT CCAGCTTCTG GTGCTTCCAG TACCACTGAT 
GTGGGCAGCG TGTTTGATTC TGAGCATTCA AGCCCTTTTC ACTCAAGCAG CGATACCATC 
TTTATCCAAG TTACACTGCC CCATGGCCCA AGATCTGCTT CAGTATCCTC AATAAATTTA 
ACTAAGAGCA TTGATGAAAT GCCCATCCCC CCACCAGTGC CCCCAAGAAG ACGACCAGAA 
TCCGCTCCAG CAGAATCTTC TCCCTCAAAG ATTATGCCCA AACATTTGGA TAGTCCTCCA 
GCAATTCCTC CTCGGCAACC CACATCAAAA GTCTATTCAC CACGTTACTC CGACCGGACC 
TCGATCTCTG ATCCTCCAGA AAGCCCTCCC CTTTTACCAC CACGAGAACC TGTGAGGACA 
CCTGATGTTT TCTCCAGCTC ACCACTACAT CTCCAGCCAC CCCCTTTGGG CAAAAAAAGT 
GAACATGGCA ATACTTTTTT CCCAAACAGC CCCTCCCCCT TTACCCCACC ACCTCCTCAA 
ACACCTTCTC CTCACGGCAC AAGGAGGCAC CTACCATCAC CACCATTAAC ACAAGATGTG 
GACCTTCATT CCATCCCTGC GCCTCCTGTT CCTCCACGGC AAAGCACTTC TCAACACATC 
CCAAAACTTC CTCCAAAAAC TTATAAAAGG GAACACACAC ACCCTTCCAT GCATAGAGAT 
GGACCACCTT TGTTGGAGAA TGCCCATTCC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003758385.1
CDS	1..3996
Translation	MQALQLQYEFFSEENAPKWRGLLVTALKKVQMQVHPTLASNEDALQYVEELILQLLSMLCQAQPRSVLDVEDRVQKSFPHPIDKWAIADAQSANEKRKRRNPLSLPVEKIHPLLKEVLGYKIDHQVSVYIVAVLEYISADILKLVGNYVRNIRHYEITKQDIKVAMCADKVLMDMFHQDVEDINILSLTDEEPSTSGEQTYYDLVKGFMAEVRQYIRELNLIIKVFREPFVSNSKLFSSHDVENIFSRIADVHELSVKLLGHIEDTVEMTDEGSPHPLVGSCFEDLAEELAFDPYESYAQDILRPGFHDHFLSQLSKPGAALYLQSIGEGFKEAVQYVLPRLLLAPVYHCLHYFELLKQLEEKSEDQEDKECLKQAITALLNLQSSMERICSKSLAKRRLSESACRFYSQQMKGKQLAIKKMNEIQKNIDGWEGKDIGQCCNEFIMEGTLTRVGAKHERHIFLFDGLMICCKSNHGQPRLPGASNAEYRLKEKFFMRKVQINDKDDTNEYKHAFEIILKDENSVIFSAKSAEEKNNWMAALISLQYRSTLERMLDVTMLQEEKEEQMRLPSSDLYRFAEPDSEENIVFEENMQPKSGIPIIKAGTVIKLIERLTYHMYADPNFVRTFLTTYRSFCKPQELLSLLIERFEIPEPEPTEADKIAMENGDQPLSVELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDTDLLQRLEEFIGTVRGKAMKKWVESITKIIQRKKMARDNGPGHNITFESSPPAVEWHISRPGHTETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIVEAENLEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHELSEDHYKKYLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLKRHGKELINFSKRRKVAEITGEIQQYQNQPYCLRVESDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRNPKPLPRFPKKYSYPLKSPGVRPSNPRPGTMRHPTPLQQEPRKISYSRIPESETESAASAPNSPRTPLTPPPASGASSTTDVGSVFDSEHSSPFHSSSDTIFIQVTLPHGPRSASVSSINLTKSIDEMPIPPPVPPRRRPESAPAESSPSKIMPKHLDSPPAIPPRQPTSKVYSPRYSDRTSISDPPESPPLLPPREPVRTPDVFSSSPLHLQPPPLGKKSEHGNTFFPNSPSPFTPPPPQTPSPHGTRRHLPSPPLTQDVDLHSIPAPPVPPRQSTSQHIPKLPPKTYKREHTHPSMHRDGPPLLENAHSS

Target ORF information:

RefSeq Version	XM_003758337.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii son of sevenless homolog 1 (Drosophila) (SOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003758337.2

ORF Insert Sequence:

ATGCAGGCGC TGCAGCTCCA GTACGAGTTC TTCAGCGAGG AAAACGCGCC CAAGTGGAGG 
GGGCTGCTGG TGACGGCCCT GAAAAAGGTC CAGATGCAAG TCCATCCTAC ACTTGCTTCA 
AATGAGGATG CTCTCCAGTA TGTTGAAGAG TTAATTTTAC AGTTGTTAAG TATGCTGTGC 
CAAGCTCAAC CCCGAAGCGT TTTAGATGTT GAGGATCGTG TACAAAAAAG TTTTCCTCAT 
CCAATTGATA AGTGGGCGAT AGCTGATGCT CAATCCGCTA ATGAAAAGAG GAAGCGAAGA 
AATCCATTAT CTCTCCCAGT AGAAAAAATT CACCCTTTAT TAAAGGAAGT TCTAGGATAT 
AAAATTGACC ACCAGGTTTC TGTTTACATA GTGGCAGTAT TAGAATACAT TTCTGCAGAT 
ATTTTAAAGC TGGTTGGAAA TTATGTACGG AATATACGAC ATTATGAAAT TACAAAACAG 
GACATCAAAG TAGCAATGTG TGCTGATAAG GTATTGATGG ATATGTTTCA TCAAGATGTA 
GAAGATATAA ATATATTATC TTTAACTGAT GAAGAACCTT CCACTTCAGG GGAACAAACG 
TACTATGACT TGGTAAAAGG ATTCATGGCT GAAGTTCGAC AATATATAAG GGAACTTAAT 
CTCATTATTA AAGTTTTTAG AGAACCATTT GTCTCCAATT CAAAATTATT TTCTTCTCAC 
GATGTAGAAA ATATATTTAG TCGTATAGCA GATGTTCATG AACTCAGTGT AAAATTATTG 
GGCCATATAG AAGACACTGT AGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTTGGT 
AGCTGCTTTG AAGACTTAGC AGAGGAATTG GCATTTGATC CATATGAATC ATATGCTCAA 
GACATATTGC GACCTGGTTT TCATGATCAC TTTCTTAGTC AGTTATCCAA GCCTGGGGCA 
GCCCTCTACT TGCAGTCAAT AGGTGAAGGT TTCAAAGAAG CTGTTCAGTA TGTTTTACCC 
AGGCTACTTC TAGCTCCTGT GTACCACTGT CTACATTACT TTGAACTTTT AAAGCAGTTA 
GAAGAGAAGA GTGAAGACCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACGGCTTTG 
CTTAACCTTC AGAGCAGTAT GGAAAGAATA TGTTCCAAAA GTCTTGCAAA ACGGAGATTG 
AGCGAATCTG CATGTCGATT TTATAGCCAA CAGATGAAGG GAAAACAACT AGCAATAAAG 
AAAATGAATG AGATTCAGAA GAACATTGAT GGGTGGGAGG GAAAAGACAT TGGACAGTGT 
TGCAATGAGT TTATCATGGA AGGAACTCTC ACACGTGTAG GTGCCAAACA TGAGAGACAC 
ATATTTCTCT TTGATGGTCT GATGATTTGC TGTAAATCAA ATCATGGTCA GCCAAGACTT 
CCTGGTGCTA GCAATGCTGA GTATCGTCTC AAAGAGAAGT TTTTTATGCG CAAGGTACAA 
ATCAATGATA AGGATGACAC TAATGAATAC AAACATGCCT TTGAAATAAT CTTAAAAGAT 
GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAGGAGA AAAACAACTG GATGGCAGCA 
CTGATATCAT TACAATATCG TAGTACTCTG GAAAGGATGC TGGATGTAAC AATGTTACAA 
GAGGAGAAGG AAGAGCAGAT GAGGCTTCCA AGTTCAGATC TTTATAGATT TGCAGAACCA 
GATTCTGAAG AAAACATAGT ATTTGAAGAA AACATGCAAC CCAAATCTGG AATTCCAATC 
ATCAAAGCAG GAACCGTTAT CAAACTTATA GAGCGACTCA CCTACCATAT GTATGCTGAT 
CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCTT TCTGCAAGCC TCAAGAGCTA 
CTGAGTCTTC TAATAGAGAG GTTTGAAATT CCAGAGCCAG AGCCAACAGA AGCTGATAAG 
ATAGCTATGG AGAATGGAGA TCAACCCCTG AGTGTGGAAC TAAAACGATT TAGAAAAGAA 
TATATACAAC CCGTACAACT TCGAGTACTA AATGTATGTC GGCATTGGGT AGAACATCAC 
TTCTATGATT TTGAAAGGGA TACAGATCTT TTGCAACGAC TGGAAGAATT TATTGGAACA 
GTAAGGGGCA AAGCAATGAA GAAATGGGTT GAATCTATCA CTAAAATCAT TCAACGGAAA 
AAAATGGCAA GAGACAATGG ACCAGGACAT AATATTACAT TTGAAAGTTC ACCACCTGCA 
GTTGAATGGC ATATAAGCAG ACCTGGACAC ACAGAAACTT TTGATCTGCT CACCTTGCAC 
CCAATAGAAA TTGCTCGACA GCTCACTTTA CTTGAGTCAG ATCTCTACAG AGCTGTACAG 
CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAC 
CTTCTGAAAA TGATTCGCCA CACAACTAAT CTTACTTTGT GGTTTGAAAA GTGTATTGTA 
GAAGCAGAAA ACCTAGAGGA AAGAGTAGCT GTGGTGAGCC GAATAATTGA GATTCTTCAA 
GTCTTTCAAG AACTGAACAA CTTTAATGGT GTACTTGAGG TTGTCAGTGC CATGAATTCA 
TCACCTGTTT ATAGACTGGA CCACACGTTT GAGCAAATTC CAAGTCGCCA AAAGAAAATT 
CTAGAGGAAG CTCATGAACT GAGTGAAGAT CACTATAAAA AATATTTGGC AAAACTCAGG 
TCTATTAATC CACCATGTGT GCCTTTCTTT GGGATTTATT TGACAAATAT CTTGAAAACG 
GAAGAAGGCA ATCCTGAGGT TTTGAAAAGA CATGGAAAAG AACTCATAAA CTTTAGCAAA 
AGAAGAAAAG TTGCAGAAAT AACAGGAGAG ATACAGCAGT ACCAAAATCA ACCATATTGT 
TTGCGAGTAG AATCTGATAT CAAAAGATTC TTTGAAAATT TGAATCCAAT GGGAAATAGC 
ATGGAAAAAG AATTCACAGA TTATCTTTTC AACAAGTCAC TGGAAATTGA ACCAAGAAAC 
CCTAAGCCTC TACCGAGATT TCCAAAAAAA TATAGTTATC CCCTCAAGTC TCCTGGTGTT 
CGTCCATCAA ACCCAAGACC AGGTACCATG AGACACCCCA CACCACTGCA GCAGGAACCA 
AGGAAGATCA GTTACAGCCG CATCCCTGAA AGTGAAACAG AAAGTGCAGC ATCAGCACCT 
AATTCTCCAC GGACCCCGTT AACACCTCCT CCAGCTTCTG GTGCTTCCAG TACCACTGAT 
GTGGGCAGCG TGTTTGATTC TGAGCATTCA AGCCCTTTTC ACTCAAGCAG CGATACCATC 
TTTATCCAAG TTACACTGCC CCATGGCCCA AGATCTGCTT CAGTATCCTC AATAAATTTA 
ACTAAGAGCA TTGATGAAAT GCCCATCCCC CCACCAGTGC CCCCAAGAAG ACGACCAGAA 
TCCGCTCCAG CAGAATCTTC TCCCTCAAAG ATTATGCCCA AACATTTGGA TAGTCCTCCA 
GCAATTCCTC CTCGGCAACC CACATCAAAA GTCTATTCAC CACGTTACTC CGACCGGACC 
TCGATCTCTG ATCCTCCAGA AAGCCCTCCC CTTTTACCAC CACGAGAACC TGTGAGGACA 
CCTGATGTTT TCTCCAGCTC ACCACTACAT CTCCAGCCAC CCCCTTTGGG CAAAAAAAGT 
GAACATGGCA ATACTTTTTT CCCAAACAGC CCCTCCCCCT TTACCCCACC ACCTCCTCAA 
ACACCTTCTC CTCACGGCAC AAGGAGGCAC CTACCATCAC CACCATTAAC ACAAGATGTG 
GACCTTCATT CCATCCCTGC GCCTCCTGTT CCTCCACGGC AAAGCACTTC TCAACACATC 
CCAAAACTTC CTCCAAAAAC TTATAAAAGG GAACACACAC ACCCTTCCAT GCATAGAGAT 
GGACCACCTT TGTTGGAGAA TGCCCATTCC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SOS1 ( XM_003758337.4 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003758385.1 Sarcophilus harrisii SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X1, mRNA.

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CloneID	OSa120072
Clone ID Related Accession (Same CDS sequence)	XM_003758337.3 , XM_003758337.2 , XM_003758337.4
Accession Version	XM_003758337.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	son of sevenless homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_003758337.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC TGCAGCTCCA GTACGAGTTC TTCAGCGAGG AAAACGCGCC CAAGTGGAGG 
GGGCTGCTGG TGACGGCCCT GAAAAAGGTC CAGATGCAAG TCCATCCTAC ACTTGCTTCA 
AATGAGGATG CTCTCCAGTA TGTTGAAGAG TTAATTTTAC AGTTGTTAAG TATGCTGTGC 
CAAGCTCAAC CCCGAAGCGT TTTAGATGTT GAGGATCGTG TACAAAAAAG TTTTCCTCAT 
CCAATTGATA AGTGGGCGAT AGCTGATGCT CAATCCGCTA ATGAAAAGAG GAAGCGAAGA 
AATCCATTAT CTCTCCCAGT AGAAAAAATT CACCCTTTAT TAAAGGAAGT TCTAGGATAT 
AAAATTGACC ACCAGGTTTC TGTTTACATA GTGGCAGTAT TAGAATACAT TTCTGCAGAT 
ATTTTAAAGC TGGTTGGAAA TTATGTACGG AATATACGAC ATTATGAAAT TACAAAACAG 
GACATCAAAG TAGCAATGTG TGCTGATAAG GTATTGATGG ATATGTTTCA TCAAGATGTA 
GAAGATATAA ATATATTATC TTTAACTGAT GAAGAACCTT CCACTTCAGG GGAACAAACG 
TACTATGACT TGGTAAAAGG ATTCATGGCT GAAGTTCGAC AATATATAAG GGAACTTAAT 
CTCATTATTA AAGTTTTTAG AGAACCATTT GTCTCCAATT CAAAATTATT TTCTTCTCAC 
GATGTAGAAA ATATATTTAG TCGTATAGCA GATGTTCATG AACTCAGTGT AAAATTATTG 
GGCCATATAG AAGACACTGT AGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTTGGT 
AGCTGCTTTG AAGACTTAGC AGAGGAATTG GCATTTGATC CATATGAATC ATATGCTCAA 
GACATATTGC GACCTGGTTT TCATGATCAC TTTCTTAGTC AGTTATCCAA GCCTGGGGCA 
GCCCTCTACT TGCAGTCAAT AGGTGAAGGT TTCAAAGAAG CTGTTCAGTA TGTTTTACCC 
AGGCTACTTC TAGCTCCTGT GTACCACTGT CTACATTACT TTGAACTTTT AAAGCAGTTA 
GAAGAGAAGA GTGAAGACCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACGGCTTTG 
CTTAACCTTC AGAGCAGTAT GGAAAGAATA TGTTCCAAAA GTCTTGCAAA ACGGAGATTG 
AGCGAATCTG CATGTCGATT TTATAGCCAA CAGATGAAGG GAAAACAACT AGCAATAAAG 
AAAATGAATG AGATTCAGAA GAACATTGAT GGGTGGGAGG GAAAAGACAT TGGACAGTGT 
TGCAATGAGT TTATCATGGA AGGAACTCTC ACACGTGTAG GTGCCAAACA TGAGAGACAC 
ATATTTCTCT TTGATGGTCT GATGATTTGC TGTAAATCAA ATCATGGTCA GCCAAGACTT 
CCTGGTGCTA GCAATGCTGA GTATCGTCTC AAAGAGAAGT TTTTTATGCG CAAGGTACAA 
ATCAATGATA AGGATGACAC TAATGAATAC AAACATGCCT TTGAAATAAT CTTAAAAGAT 
GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAGGAGA AAAACAACTG GATGGCAGCA 
CTGATATCAT TACAATATCG TAGTACTCTG GAAAGGATGC TGGATGTAAC AATGTTACAA 
GAGGAGAAGG AAGAGCAGAT GAGGCTTCCA AGTTCAGATC TTTATAGATT TGCAGAACCA 
GATTCTGAAG AAAACATAGT ATTTGAAGAA AACATGCAAC CCAAATCTGG AATTCCAATC 
ATCAAAGCAG GAACCGTTAT CAAACTTATA GAGCGACTCA CCTACCATAT GTATGCTGAT 
CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCTT TCTGCAAGCC TCAAGAGCTA 
CTGAGTCTTC TAATAGAGAG GTTTGAAATT CCAGAGCCAG AGCCAACAGA AGCTGATAAG 
ATAGCTATGG AGAATGGAGA TCAACCCCTG AGTGTGGAAC TAAAACGATT TAGAAAAGAA 
TATATACAAC CCGTACAACT TCGAGTACTA AATGTATGTC GGCATTGGGT AGAACATCAC 
TTCTATGATT TTGAAAGGGA TACAGATCTT TTGCAACGAC TGGAAGAATT TATTGGAACA 
GTAAGGGGCA AAGCAATGAA GAAATGGGTT GAATCTATCA CTAAAATCAT TCAACGGAAA 
AAAATGGCAA GAGACAATGG ACCAGGACAT AATATTACAT TTGAAAGTTC ACCACCTGCA 
GTTGAATGGC ATATAAGCAG ACCTGGACAC ACAGAAACTT TTGATCTGCT CACCTTGCAC 
CCAATAGAAA TTGCTCGACA GCTCACTTTA CTTGAGTCAG ATCTCTACAG AGCTGTACAG 
CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAC 
CTTCTGAAAA TGATTCGCCA CACAACTAAT CTTACTTTGT GGTTTGAAAA GTGTATTGTA 
GAAGCAGAAA ACCTAGAGGA AAGAGTAGCT GTGGTGAGCC GAATAATTGA GATTCTTCAA 
GTCTTTCAAG AACTGAACAA CTTTAATGGT GTACTTGAGG TTGTCAGTGC CATGAATTCA 
TCACCTGTTT ATAGACTGGA CCACACGTTT GAGCAAATTC CAAGTCGCCA AAAGAAAATT 
CTAGAGGAAG CTCATGAACT GAGTGAAGAT CACTATAAAA AATATTTGGC AAAACTCAGG 
TCTATTAATC CACCATGTGT GCCTTTCTTT GGGATTTATT TGACAAATAT CTTGAAAACG 
GAAGAAGGCA ATCCTGAGGT TTTGAAAAGA CATGGAAAAG AACTCATAAA CTTTAGCAAA 
AGAAGAAAAG TTGCAGAAAT AACAGGAGAG ATACAGCAGT ACCAAAATCA ACCATATTGT 
TTGCGAGTAG AATCTGATAT CAAAAGATTC TTTGAAAATT TGAATCCAAT GGGAAATAGC 
ATGGAAAAAG AATTCACAGA TTATCTTTTC AACAAGTCAC TGGAAATTGA ACCAAGAAAC 
CCTAAGCCTC TACCGAGATT TCCAAAAAAA TATAGTTATC CCCTCAAGTC TCCTGGTGTT 
CGTCCATCAA ACCCAAGACC AGGTACCATG AGACACCCCA CACCACTGCA GCAGGAACCA 
AGGAAGATCA GTTACAGCCG CATCCCTGAA AGTGAAACAG AAAGTGCAGC ATCAGCACCT 
AATTCTCCAC GGACCCCGTT AACACCTCCT CCAGCTTCTG GTGCTTCCAG TACCACTGAT 
GTGGGCAGCG TGTTTGATTC TGAGCATTCA AGCCCTTTTC ACTCAAGCAG CGATACCATC 
TTTATCCAAG TTACACTGCC CCATGGCCCA AGATCTGCTT CAGTATCCTC AATAAATTTA 
ACTAAGAGCA TTGATGAAAT GCCCATCCCC CCACCAGTGC CCCCAAGAAG ACGACCAGAA 
TCCGCTCCAG CAGAATCTTC TCCCTCAAAG ATTATGCCCA AACATTTGGA TAGTCCTCCA 
GCAATTCCTC CTCGGCAACC CACATCAAAA GTCTATTCAC CACGTTACTC CGACCGGACC 
TCGATCTCTG ATCCTCCAGA AAGCCCTCCC CTTTTACCAC CACGAGAACC TGTGAGGACA 
CCTGATGTTT TCTCCAGCTC ACCACTACAT CTCCAGCCAC CCCCTTTGGG CAAAAAAAGT 
GAACATGGCA ATACTTTTTT CCCAAACAGC CCCTCCCCCT TTACCCCACC ACCTCCTCAA 
ACACCTTCTC CTCACGGCAC AAGGAGGCAC CTACCATCAC CACCATTAAC ACAAGATGTG 
GACCTTCATT CCATCCCTGC GCCTCCTGTT CCTCCACGGC AAAGCACTTC TCAACACATC 
CCAAAACTTC CTCCAAAAAC TTATAAAAGG GAACACACAC ACCCTTCCAT GCATAGAGAT 
GGACCACCTT TGTTGGAGAA TGCCCATTCC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003758385.1
CDS	267..4262
Translation	MQALQLQYEFFSEENAPKWRGLLVTALKKVQMQVHPTLASNEDALQYVEELILQLLSMLCQAQPRSVLDVEDRVQKSFPHPIDKWAIADAQSANEKRKRRNPLSLPVEKIHPLLKEVLGYKIDHQVSVYIVAVLEYISADILKLVGNYVRNIRHYEITKQDIKVAMCADKVLMDMFHQDVEDINILSLTDEEPSTSGEQTYYDLVKGFMAEVRQYIRELNLIIKVFREPFVSNSKLFSSHDVENIFSRIADVHELSVKLLGHIEDTVEMTDEGSPHPLVGSCFEDLAEELAFDPYESYAQDILRPGFHDHFLSQLSKPGAALYLQSIGEGFKEAVQYVLPRLLLAPVYHCLHYFELLKQLEEKSEDQEDKECLKQAITALLNLQSSMERICSKSLAKRRLSESACRFYSQQMKGKQLAIKKMNEIQKNIDGWEGKDIGQCCNEFIMEGTLTRVGAKHERHIFLFDGLMICCKSNHGQPRLPGASNAEYRLKEKFFMRKVQINDKDDTNEYKHAFEIILKDENSVIFSAKSAEEKNNWMAALISLQYRSTLERMLDVTMLQEEKEEQMRLPSSDLYRFAEPDSEENIVFEENMQPKSGIPIIKAGTVIKLIERLTYHMYADPNFVRTFLTTYRSFCKPQELLSLLIERFEIPEPEPTEADKIAMENGDQPLSVELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDTDLLQRLEEFIGTVRGKAMKKWVESITKIIQRKKMARDNGPGHNITFESSPPAVEWHISRPGHTETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIVEAENLEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHELSEDHYKKYLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLKRHGKELINFSKRRKVAEITGEIQQYQNQPYCLRVESDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRNPKPLPRFPKKYSYPLKSPGVRPSNPRPGTMRHPTPLQQEPRKISYSRIPESETESAASAPNSPRTPLTPPPASGASSTTDVGSVFDSEHSSPFHSSSDTIFIQVTLPHGPRSASVSSINLTKSIDEMPIPPPVPPRRRPESAPAESSPSKIMPKHLDSPPAIPPRQPTSKVYSPRYSDRTSISDPPESPPLLPPREPVRTPDVFSSSPLHLQPPPLGKKSEHGNTFFPNSPSPFTPPPPQTPSPHGTRRHLPSPPLTQDVDLHSIPAPPVPPRQSTSQHIPKLPPKTYKREHTHPSMHRDGPPLLENAHSS

Target ORF information:

RefSeq Version	XM_003758337.4
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003758337.4

ORF Insert Sequence:

ATGCAGGCGC TGCAGCTCCA GTACGAGTTC TTCAGCGAGG AAAACGCGCC CAAGTGGAGG 
GGGCTGCTGG TGACGGCCCT GAAAAAGGTC CAGATGCAAG TCCATCCTAC ACTTGCTTCA 
AATGAGGATG CTCTCCAGTA TGTTGAAGAG TTAATTTTAC AGTTGTTAAG TATGCTGTGC 
CAAGCTCAAC CCCGAAGCGT TTTAGATGTT GAGGATCGTG TACAAAAAAG TTTTCCTCAT 
CCAATTGATA AGTGGGCGAT AGCTGATGCT CAATCCGCTA ATGAAAAGAG GAAGCGAAGA 
AATCCATTAT CTCTCCCAGT AGAAAAAATT CACCCTTTAT TAAAGGAAGT TCTAGGATAT 
AAAATTGACC ACCAGGTTTC TGTTTACATA GTGGCAGTAT TAGAATACAT TTCTGCAGAT 
ATTTTAAAGC TGGTTGGAAA TTATGTACGG AATATACGAC ATTATGAAAT TACAAAACAG 
GACATCAAAG TAGCAATGTG TGCTGATAAG GTATTGATGG ATATGTTTCA TCAAGATGTA 
GAAGATATAA ATATATTATC TTTAACTGAT GAAGAACCTT CCACTTCAGG GGAACAAACG 
TACTATGACT TGGTAAAAGG ATTCATGGCT GAAGTTCGAC AATATATAAG GGAACTTAAT 
CTCATTATTA AAGTTTTTAG AGAACCATTT GTCTCCAATT CAAAATTATT TTCTTCTCAC 
GATGTAGAAA ATATATTTAG TCGTATAGCA GATGTTCATG AACTCAGTGT AAAATTATTG 
GGCCATATAG AAGACACTGT AGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTTGGT 
AGCTGCTTTG AAGACTTAGC AGAGGAATTG GCATTTGATC CATATGAATC ATATGCTCAA 
GACATATTGC GACCTGGTTT TCATGATCAC TTTCTTAGTC AGTTATCCAA GCCTGGGGCA 
GCCCTCTACT TGCAGTCAAT AGGTGAAGGT TTCAAAGAAG CTGTTCAGTA TGTTTTACCC 
AGGCTACTTC TAGCTCCTGT GTACCACTGT CTACATTACT TTGAACTTTT AAAGCAGTTA 
GAAGAGAAGA GTGAAGACCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACGGCTTTG 
CTTAACCTTC AGAGCAGTAT GGAAAGAATA TGTTCCAAAA GTCTTGCAAA ACGGAGATTG 
AGCGAATCTG CATGTCGATT TTATAGCCAA CAGATGAAGG GAAAACAACT AGCAATAAAG 
AAAATGAATG AGATTCAGAA GAACATTGAT GGGTGGGAGG GAAAAGACAT TGGACAGTGT 
TGCAATGAGT TTATCATGGA AGGAACTCTC ACACGTGTAG GTGCCAAACA TGAGAGACAC 
ATATTTCTCT TTGATGGTCT GATGATTTGC TGTAAATCAA ATCATGGTCA GCCAAGACTT 
CCTGGTGCTA GCAATGCTGA GTATCGTCTC AAAGAGAAGT TTTTTATGCG CAAGGTACAA 
ATCAATGATA AGGATGACAC TAATGAATAC AAACATGCCT TTGAAATAAT CTTAAAAGAT 
GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAGGAGA AAAACAACTG GATGGCAGCA 
CTGATATCAT TACAATATCG TAGTACTCTG GAAAGGATGC TGGATGTAAC AATGTTACAA 
GAGGAGAAGG AAGAGCAGAT GAGGCTTCCA AGTTCAGATC TTTATAGATT TGCAGAACCA 
GATTCTGAAG AAAACATAGT ATTTGAAGAA AACATGCAAC CCAAATCTGG AATTCCAATC 
ATCAAAGCAG GAACCGTTAT CAAACTTATA GAGCGACTCA CCTACCATAT GTATGCTGAT 
CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCTT TCTGCAAGCC TCAAGAGCTA 
CTGAGTCTTC TAATAGAGAG GTTTGAAATT CCAGAGCCAG AGCCAACAGA AGCTGATAAG 
ATAGCTATGG AGAATGGAGA TCAACCCCTG AGTGTGGAAC TAAAACGATT TAGAAAAGAA 
TATATACAAC CCGTACAACT TCGAGTACTA AATGTATGTC GGCATTGGGT AGAACATCAC 
TTCTATGATT TTGAAAGGGA TACAGATCTT TTGCAACGAC TGGAAGAATT TATTGGAACA 
GTAAGGGGCA AAGCAATGAA GAAATGGGTT GAATCTATCA CTAAAATCAT TCAACGGAAA 
AAAATGGCAA GAGACAATGG ACCAGGACAT AATATTACAT TTGAAAGTTC ACCACCTGCA 
GTTGAATGGC ATATAAGCAG ACCTGGACAC ACAGAAACTT TTGATCTGCT CACCTTGCAC 
CCAATAGAAA TTGCTCGACA GCTCACTTTA CTTGAGTCAG ATCTCTACAG AGCTGTACAG 
CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAC 
CTTCTGAAAA TGATTCGCCA CACAACTAAT CTTACTTTGT GGTTTGAAAA GTGTATTGTA 
GAAGCAGAAA ACCTAGAGGA AAGAGTAGCT GTGGTGAGCC GAATAATTGA GATTCTTCAA 
GTCTTTCAAG AACTGAACAA CTTTAATGGT GTACTTGAGG TTGTCAGTGC CATGAATTCA 
TCACCTGTTT ATAGACTGGA CCACACGTTT GAGCAAATTC CAAGTCGCCA AAAGAAAATT 
CTAGAGGAAG CTCATGAACT GAGTGAAGAT CACTATAAAA AATATTTGGC AAAACTCAGG 
TCTATTAATC CACCATGTGT GCCTTTCTTT GGGATTTATT TGACAAATAT CTTGAAAACG 
GAAGAAGGCA ATCCTGAGGT TTTGAAAAGA CATGGAAAAG AACTCATAAA CTTTAGCAAA 
AGAAGAAAAG TTGCAGAAAT AACAGGAGAG ATACAGCAGT ACCAAAATCA ACCATATTGT 
TTGCGAGTAG AATCTGATAT CAAAAGATTC TTTGAAAATT TGAATCCAAT GGGAAATAGC 
ATGGAAAAAG AATTCACAGA TTATCTTTTC AACAAGTCAC TGGAAATTGA ACCAAGAAAC 
CCTAAGCCTC TACCGAGATT TCCAAAAAAA TATAGTTATC CCCTCAAGTC TCCTGGTGTT 
CGTCCATCAA ACCCAAGACC AGGTACCATG AGACACCCCA CACCACTGCA GCAGGAACCA 
AGGAAGATCA GTTACAGCCG CATCCCTGAA AGTGAAACAG AAAGTGCAGC ATCAGCACCT 
AATTCTCCAC GGACCCCGTT AACACCTCCT CCAGCTTCTG GTGCTTCCAG TACCACTGAT 
GTGGGCAGCG TGTTTGATTC TGAGCATTCA AGCCCTTTTC ACTCAAGCAG CGATACCATC 
TTTATCCAAG TTACACTGCC CCATGGCCCA AGATCTGCTT CAGTATCCTC AATAAATTTA 
ACTAAGAGCA TTGATGAAAT GCCCATCCCC CCACCAGTGC CCCCAAGAAG ACGACCAGAA 
TCCGCTCCAG CAGAATCTTC TCCCTCAAAG ATTATGCCCA AACATTTGGA TAGTCCTCCA 
GCAATTCCTC CTCGGCAACC CACATCAAAA GTCTATTCAC CACGTTACTC CGACCGGACC 
TCGATCTCTG ATCCTCCAGA AAGCCCTCCC CTTTTACCAC CACGAGAACC TGTGAGGACA 
CCTGATGTTT TCTCCAGCTC ACCACTACAT CTCCAGCCAC CCCCTTTGGG CAAAAAAAGT 
GAACATGGCA ATACTTTTTT CCCAAACAGC CCCTCCCCCT TTACCCCACC ACCTCCTCAA 
ACACCTTCTC CTCACGGCAC AAGGAGGCAC CTACCATCAC CACCATTAAC ACAAGATGTG 
GACCTTCATT CCATCCCTGC GCCTCCTGTT CCTCCACGGC AAAGCACTTC TCAACACATC 
CCAAAACTTC CTCCAAAAAC TTATAAAAGG GAACACACAC ACCCTTCCAT GCATAGAGAT 
GGACCACCTT TGTTGGAGAA TGCCCATTCC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SOS1 ( XM_003758336.4 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003758384.1 Sarcophilus harrisii SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X2, mRNA.

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CloneID	OSa120073
Clone ID Related Accession (Same CDS sequence)	XM_003758336.4 , XM_003758336.3 , XM_003758336.2
Accession Version	XM_003758336.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	son of sevenless homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_003758336.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC TGCAGCTCCA GTACGAGTTC TTCAGCGAGG AAAACGCGCC CAAGTGGAGG 
GGGCTGCTGG TGACGGCCCT GAAAAAGGTC CAGATGCAAG TCCATCCTAC ACTTGCTTCA 
AATGAGGATG CTCTCCAGTA TGTTGAAGAG TTAATTTTAC AGTTGTTAAG TATGCTGTGC 
CAAGCTCAAC CCCGAAGCGT TTTAGATGTT GAGGATCGTG TACAAAAAAG TTTTCCTCAT 
CCAATTGATA AGTGGGCGAT AGCTGATGCT CAATCCGCTA ATGAAAAGAG GAAGCGAAGA 
AATCCATTAT CTCTCCCAGT AGAAAAAATT CACCCTTTAT TAAAGGAAGT TCTAGGATAT 
AAAATTGACC ACCAGGTTTC TGTTTACATA GTGGCAGTAT TAGAATACAT TTCTGCAGAT 
ATTTTAAAGC TGGTTGGAAA TTATGTACGG AATATACGAC ATTATGAAAT TACAAAACAG 
GACATCAAAG TAGCAATGTG TGCTGATAAG GTATTGATGG ATATGTTTCA TCAAGATGTA 
GAAGATATAA ATATATTATC TTTAACTGAT GAAGAACCTT CCACTTCAGG GGAACAAACG 
TACTATGACT TGGTAAAAGG ATTCATGGCT GAAGTTCGAC AATATATAAG GGAACTTAAT 
CTCATTATTA AAGTTTTTAG AGAACCATTT GTCTCCAATT CAAAATTATT TTCTTCTCAC 
GATGTAGAAA ATATATTTAG TCGTATAGCA GATGTTCATG AACTCAGTGT AAAATTATTG 
GGCCATATAG AAGACACTGT AGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTTGGT 
AGCTGCTTTG AAGACTTAGC AGAGGAATTG GCATTTGATC CATATGAATC ATATGCTCAA 
GACATATTGC GACCTGGTTT TCATGATCAC TTTCTTAGTC AGTTATCCAA GCCTGGGGCA 
GCCCTCTACT TGCAGTCAAT AGGTGAAGGT TTCAAAGAAG CTGTTCAGTA TGTTTTACCC 
AGGCTACTTC TAGCTCCTGT GTACCACTGT CTACATTACT TTGAACTTTT AAAGCAGTTA 
GAAGAGAAGA GTGAAGACCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACGGCTTTG 
CTTAACCTTC AGAGCAGTAT GGAAAGAATA TGTTCCAAAA GTCTTGCAAA ACGGAGATTG 
AGCGAATCTG CATGTCGATT TTATAGCCAA CAGATGAAGG GAAAACAACT AGCAATAAAG 
AAAATGAATG AGATTCAGAA GAACATTGAT GGGTGGGAGG GAAAAGACAT TGGACAGTGT 
TGCAATGAGT TTATCATGGA AGGAACTCTC ACACGTGTAG GTGCCAAACA TGAGAGACAC 
ATATTTCTCT TTGATGGTCT GATGATTTGC TGTAAATCAA ATCATGGTCA GCCAAGACTT 
CCTGGTGCTA GCAATGCTGA GTATCGTCTC AAAGAGAAGT TTTTTATGCG CAAGGTACAA 
ATCAATGATA AGGATGACAC TAATGAATAC AAACATGCCT TTGAAATAAT CTTAAAAGAT 
GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAGGAGA AAAACAACTG GATGGCAGCA 
CTGATATCAT TACAATATCG TAGTACTCTG GAAAGGATGC TGGATGTAAC AATGTTACAA 
GAGGAGAAGG AAGAGCAGAT GAGGCTTCCA AGTTCAGATC TTTATAGATT TGCAGAACCA 
GATTCTGAAG AAAACATAGT ATTTGAAGAA AACATGCAAC CCAAATCTGG AATTCCAATC 
ATCAAAGCAG GAACCGTTAT CAAACTTATA GAGCGACTCA CCTACCATAT GTATGCTGAT 
CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCTT TCTGCAAGCC TCAAGAGCTA 
CTGAGTCTTC TAATAGAGAG GTTTGAAATT CCAGAGCCAG AGCCAACAGA AGCTGATAAG 
ATAGCTATGG AGAATGGAGA TCAACCCCTG AGTGTGGAAC TAAAACGATT TAGAAAAGAA 
TATATACAAC CCGTACAACT TCGAGTACTA AATGTATGTC GGCATTGGGT AGAACATCAC 
TTCTATGATT TTGAAAGGGA TACAGATCTT TTGCAACGAC TGGAAGAATT TATTGGAACA 
GTAAGGGGCA AAGCAATGAA GAAATGGGTT GAATCTATCA CTAAAATCAT TCAACGGAAA 
AAAATGGCAA GAGACAATGG ACCAGGACAT AATATTACAT TTGAAAGTTC ACCACCTGCA 
GTTGAATGGC ATATAAGCAG ACCTGGACAC ACAGAAACTT TTGATCTGCT CACCTTGCAC 
CCAATAGAAA TTGCTCGACA GCTCACTTTA CTTGAGTCAG ATCTCTACAG AGCTGTACAG 
CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAC 
CTTCTGAAAA TGATTCGCCA CACAACTAAT CTTACTTTGT GGTTTGAAAA GTGTATTGTA 
GAAGCAGAAA ACCTAGAGGA AAGAGTAGCT GTGGTGAGCC GAATAATTGA GATTCTTCAA 
GTCTTTCAAG AACTGAACAA CTTTAATGGT GTACTTGAGG TTGTCAGTGC CATGAATTCA 
TCACCTGTTT ATAGACTGGA CCACACGTTT GAGCAAATTC CAAGTCGCCA AAAGAAAATT 
CTAGAGGAAG CTCATGAACT GAGTGAAGAT CACTATAAAA AATATTTGGC AAAACTCAGG 
TCTATTAATC CACCATGTGT GCCTTTCTTT GGGATTTATT TGACAAATAT CTTGAAAACG 
GAAGAAGGCA ATCCTGAGGT TTTGAAAAGA CATGGAAAAG AACTCATAAA CTTTAGCAAA 
AGAAGAAAAG TTGCAGAAAT AACAGGAGAG ATACAGCAGT ACCAAAATCA ACCATATTGT 
TTGCGAGTAG AATCTGATAT CAAAAGATTC TTTGAAAATT TGAATCCAAT GGGAAATAGC 
ATGGAAAAAG AATTCACAGA TTATCTTTTC AACAAGTCAC TGGAAATTGA ACCAAGAAAC 
CCTAAGCCTC TACCGAGATT TCCAAAAAAA TATAGTTATC CCCTCAAGTC TCCTGGTGTT 
CGTCCATCAA ACCCAAGACC AGGTACCATG AGACACCCCA CACCACTGCA GCAGGAACCA 
AGGAAGATCA GTTACAGCCG CATCCCTGAA AGTGAAACAG AAAGTGCAGC ATCAGCACCT 
AATTCTCCAC GGACCCCGTT AACACCTCCT CCAGCTTCTG GTGCTTCCAG TACCACTGAT 
GTGGGCAGCG TGTTTGATTC TGAGCATTCA AGCCCTTTTC ACTCAAGATC TGCTTCAGTA 
TCCTCAATAA ATTTAACTAA GAGCATTGAT GAAATGCCCA TCCCCCCACC AGTGCCCCCA 
AGAAGACGAC CAGAATCCGC TCCAGCAGAA TCTTCTCCCT CAAAGATTAT GCCCAAACAT 
TTGGATAGTC CTCCAGCAAT TCCTCCTCGG CAACCCACAT CAAAAGTCTA TTCACCACGT 
TACTCCGACC GGACCTCGAT CTCTGATCCT CCAGAAAGCC CTCCCCTTTT ACCACCACGA 
GAACCTGTGA GGACACCTGA TGTTTTCTCC AGCTCACCAC TACATCTCCA GCCACCCCCT 
TTGGGCAAAA AAAGTGAACA TGGCAATACT TTTTTCCCAA ACAGCCCCTC CCCCTTTACC 
CCACCACCTC CTCAAACACC TTCTCCTCAC GGCACAAGGA GGCACCTACC ATCACCACCA 
TTAACACAAG ATGTGGACCT TCATTCCATC CCTGCGCCTC CTGTTCCTCC ACGGCAAAGC 
ACTTCTCAAC ACATCCCAAA ACTTCCTCCA AAAACTTATA AAAGGGAACA CACACACCCT 
TCCATGCATA GAGATGGACC ACCTTTGTTG GAGAATGCCC ATTCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003758384.1
CDS	267..4217
Translation	MQALQLQYEFFSEENAPKWRGLLVTALKKVQMQVHPTLASNEDALQYVEELILQLLSMLCQAQPRSVLDVEDRVQKSFPHPIDKWAIADAQSANEKRKRRNPLSLPVEKIHPLLKEVLGYKIDHQVSVYIVAVLEYISADILKLVGNYVRNIRHYEITKQDIKVAMCADKVLMDMFHQDVEDINILSLTDEEPSTSGEQTYYDLVKGFMAEVRQYIRELNLIIKVFREPFVSNSKLFSSHDVENIFSRIADVHELSVKLLGHIEDTVEMTDEGSPHPLVGSCFEDLAEELAFDPYESYAQDILRPGFHDHFLSQLSKPGAALYLQSIGEGFKEAVQYVLPRLLLAPVYHCLHYFELLKQLEEKSEDQEDKECLKQAITALLNLQSSMERICSKSLAKRRLSESACRFYSQQMKGKQLAIKKMNEIQKNIDGWEGKDIGQCCNEFIMEGTLTRVGAKHERHIFLFDGLMICCKSNHGQPRLPGASNAEYRLKEKFFMRKVQINDKDDTNEYKHAFEIILKDENSVIFSAKSAEEKNNWMAALISLQYRSTLERMLDVTMLQEEKEEQMRLPSSDLYRFAEPDSEENIVFEENMQPKSGIPIIKAGTVIKLIERLTYHMYADPNFVRTFLTTYRSFCKPQELLSLLIERFEIPEPEPTEADKIAMENGDQPLSVELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDTDLLQRLEEFIGTVRGKAMKKWVESITKIIQRKKMARDNGPGHNITFESSPPAVEWHISRPGHTETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIVEAENLEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHELSEDHYKKYLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLKRHGKELINFSKRRKVAEITGEIQQYQNQPYCLRVESDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRNPKPLPRFPKKYSYPLKSPGVRPSNPRPGTMRHPTPLQQEPRKISYSRIPESETESAASAPNSPRTPLTPPPASGASSTTDVGSVFDSEHSSPFHSRSASVSSINLTKSIDEMPIPPPVPPRRRPESAPAESSPSKIMPKHLDSPPAIPPRQPTSKVYSPRYSDRTSISDPPESPPLLPPREPVRTPDVFSSSPLHLQPPPLGKKSEHGNTFFPNSPSPFTPPPPQTPSPHGTRRHLPSPPLTQDVDLHSIPAPPVPPRQSTSQHIPKLPPKTYKREHTHPSMHRDGPPLLENAHSS

Target ORF information:

RefSeq Version	XM_003758336.4
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003758336.4

ORF Insert Sequence:

ATGCAGGCGC TGCAGCTCCA GTACGAGTTC TTCAGCGAGG AAAACGCGCC CAAGTGGAGG 
GGGCTGCTGG TGACGGCCCT GAAAAAGGTC CAGATGCAAG TCCATCCTAC ACTTGCTTCA 
AATGAGGATG CTCTCCAGTA TGTTGAAGAG TTAATTTTAC AGTTGTTAAG TATGCTGTGC 
CAAGCTCAAC CCCGAAGCGT TTTAGATGTT GAGGATCGTG TACAAAAAAG TTTTCCTCAT 
CCAATTGATA AGTGGGCGAT AGCTGATGCT CAATCCGCTA ATGAAAAGAG GAAGCGAAGA 
AATCCATTAT CTCTCCCAGT AGAAAAAATT CACCCTTTAT TAAAGGAAGT TCTAGGATAT 
AAAATTGACC ACCAGGTTTC TGTTTACATA GTGGCAGTAT TAGAATACAT TTCTGCAGAT 
ATTTTAAAGC TGGTTGGAAA TTATGTACGG AATATACGAC ATTATGAAAT TACAAAACAG 
GACATCAAAG TAGCAATGTG TGCTGATAAG GTATTGATGG ATATGTTTCA TCAAGATGTA 
GAAGATATAA ATATATTATC TTTAACTGAT GAAGAACCTT CCACTTCAGG GGAACAAACG 
TACTATGACT TGGTAAAAGG ATTCATGGCT GAAGTTCGAC AATATATAAG GGAACTTAAT 
CTCATTATTA AAGTTTTTAG AGAACCATTT GTCTCCAATT CAAAATTATT TTCTTCTCAC 
GATGTAGAAA ATATATTTAG TCGTATAGCA GATGTTCATG AACTCAGTGT AAAATTATTG 
GGCCATATAG AAGACACTGT AGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTTGGT 
AGCTGCTTTG AAGACTTAGC AGAGGAATTG GCATTTGATC CATATGAATC ATATGCTCAA 
GACATATTGC GACCTGGTTT TCATGATCAC TTTCTTAGTC AGTTATCCAA GCCTGGGGCA 
GCCCTCTACT TGCAGTCAAT AGGTGAAGGT TTCAAAGAAG CTGTTCAGTA TGTTTTACCC 
AGGCTACTTC TAGCTCCTGT GTACCACTGT CTACATTACT TTGAACTTTT AAAGCAGTTA 
GAAGAGAAGA GTGAAGACCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACGGCTTTG 
CTTAACCTTC AGAGCAGTAT GGAAAGAATA TGTTCCAAAA GTCTTGCAAA ACGGAGATTG 
AGCGAATCTG CATGTCGATT TTATAGCCAA CAGATGAAGG GAAAACAACT AGCAATAAAG 
AAAATGAATG AGATTCAGAA GAACATTGAT GGGTGGGAGG GAAAAGACAT TGGACAGTGT 
TGCAATGAGT TTATCATGGA AGGAACTCTC ACACGTGTAG GTGCCAAACA TGAGAGACAC 
ATATTTCTCT TTGATGGTCT GATGATTTGC TGTAAATCAA ATCATGGTCA GCCAAGACTT 
CCTGGTGCTA GCAATGCTGA GTATCGTCTC AAAGAGAAGT TTTTTATGCG CAAGGTACAA 
ATCAATGATA AGGATGACAC TAATGAATAC AAACATGCCT TTGAAATAAT CTTAAAAGAT 
GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAGGAGA AAAACAACTG GATGGCAGCA 
CTGATATCAT TACAATATCG TAGTACTCTG GAAAGGATGC TGGATGTAAC AATGTTACAA 
GAGGAGAAGG AAGAGCAGAT GAGGCTTCCA AGTTCAGATC TTTATAGATT TGCAGAACCA 
GATTCTGAAG AAAACATAGT ATTTGAAGAA AACATGCAAC CCAAATCTGG AATTCCAATC 
ATCAAAGCAG GAACCGTTAT CAAACTTATA GAGCGACTCA CCTACCATAT GTATGCTGAT 
CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCTT TCTGCAAGCC TCAAGAGCTA 
CTGAGTCTTC TAATAGAGAG GTTTGAAATT CCAGAGCCAG AGCCAACAGA AGCTGATAAG 
ATAGCTATGG AGAATGGAGA TCAACCCCTG AGTGTGGAAC TAAAACGATT TAGAAAAGAA 
TATATACAAC CCGTACAACT TCGAGTACTA AATGTATGTC GGCATTGGGT AGAACATCAC 
TTCTATGATT TTGAAAGGGA TACAGATCTT TTGCAACGAC TGGAAGAATT TATTGGAACA 
GTAAGGGGCA AAGCAATGAA GAAATGGGTT GAATCTATCA CTAAAATCAT TCAACGGAAA 
AAAATGGCAA GAGACAATGG ACCAGGACAT AATATTACAT TTGAAAGTTC ACCACCTGCA 
GTTGAATGGC ATATAAGCAG ACCTGGACAC ACAGAAACTT TTGATCTGCT CACCTTGCAC 
CCAATAGAAA TTGCTCGACA GCTCACTTTA CTTGAGTCAG ATCTCTACAG AGCTGTACAG 
CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAC 
CTTCTGAAAA TGATTCGCCA CACAACTAAT CTTACTTTGT GGTTTGAAAA GTGTATTGTA 
GAAGCAGAAA ACCTAGAGGA AAGAGTAGCT GTGGTGAGCC GAATAATTGA GATTCTTCAA 
GTCTTTCAAG AACTGAACAA CTTTAATGGT GTACTTGAGG TTGTCAGTGC CATGAATTCA 
TCACCTGTTT ATAGACTGGA CCACACGTTT GAGCAAATTC CAAGTCGCCA AAAGAAAATT 
CTAGAGGAAG CTCATGAACT GAGTGAAGAT CACTATAAAA AATATTTGGC AAAACTCAGG 
TCTATTAATC CACCATGTGT GCCTTTCTTT GGGATTTATT TGACAAATAT CTTGAAAACG 
GAAGAAGGCA ATCCTGAGGT TTTGAAAAGA CATGGAAAAG AACTCATAAA CTTTAGCAAA 
AGAAGAAAAG TTGCAGAAAT AACAGGAGAG ATACAGCAGT ACCAAAATCA ACCATATTGT 
TTGCGAGTAG AATCTGATAT CAAAAGATTC TTTGAAAATT TGAATCCAAT GGGAAATAGC 
ATGGAAAAAG AATTCACAGA TTATCTTTTC AACAAGTCAC TGGAAATTGA ACCAAGAAAC 
CCTAAGCCTC TACCGAGATT TCCAAAAAAA TATAGTTATC CCCTCAAGTC TCCTGGTGTT 
CGTCCATCAA ACCCAAGACC AGGTACCATG AGACACCCCA CACCACTGCA GCAGGAACCA 
AGGAAGATCA GTTACAGCCG CATCCCTGAA AGTGAAACAG AAAGTGCAGC ATCAGCACCT 
AATTCTCCAC GGACCCCGTT AACACCTCCT CCAGCTTCTG GTGCTTCCAG TACCACTGAT 
GTGGGCAGCG TGTTTGATTC TGAGCATTCA AGCCCTTTTC ACTCAAGATC TGCTTCAGTA 
TCCTCAATAA ATTTAACTAA GAGCATTGAT GAAATGCCCA TCCCCCCACC AGTGCCCCCA 
AGAAGACGAC CAGAATCCGC TCCAGCAGAA TCTTCTCCCT CAAAGATTAT GCCCAAACAT 
TTGGATAGTC CTCCAGCAAT TCCTCCTCGG CAACCCACAT CAAAAGTCTA TTCACCACGT 
TACTCCGACC GGACCTCGAT CTCTGATCCT CCAGAAAGCC CTCCCCTTTT ACCACCACGA 
GAACCTGTGA GGACACCTGA TGTTTTCTCC AGCTCACCAC TACATCTCCA GCCACCCCCT 
TTGGGCAAAA AAAGTGAACA TGGCAATACT TTTTTCCCAA ACAGCCCCTC CCCCTTTACC 
CCACCACCTC CTCAAACACC TTCTCCTCAC GGCACAAGGA GGCACCTACC ATCACCACCA 
TTAACACAAG ATGTGGACCT TCATTCCATC CCTGCGCCTC CTGTTCCTCC ACGGCAAAGC 
ACTTCTCAAC ACATCCCAAA ACTTCCTCCA AAAACTTATA AAAGGGAACA CACACACCCT 
TCCATGCATA GAGATGGACC ACCTTTGTTG GAGAATGCCC ATTCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SOS1 ( XM_003758336.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003758384.1 Sarcophilus harrisii SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa120073
Clone ID Related Accession (Same CDS sequence)	XM_003758336.4 , XM_003758336.3 , XM_003758336.2
Accession Version	XM_003758336.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	son of sevenless homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816756.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_003758336.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC TGCAGCTCCA GTACGAGTTC TTCAGCGAGG AAAACGCGCC CAAGTGGAGG 
GGGCTGCTGG TGACGGCCCT GAAAAAGGTC CAGATGCAAG TCCATCCTAC ACTTGCTTCA 
AATGAGGATG CTCTCCAGTA TGTTGAAGAG TTAATTTTAC AGTTGTTAAG TATGCTGTGC 
CAAGCTCAAC CCCGAAGCGT TTTAGATGTT GAGGATCGTG TACAAAAAAG TTTTCCTCAT 
CCAATTGATA AGTGGGCGAT AGCTGATGCT CAATCCGCTA ATGAAAAGAG GAAGCGAAGA 
AATCCATTAT CTCTCCCAGT AGAAAAAATT CACCCTTTAT TAAAGGAAGT TCTAGGATAT 
AAAATTGACC ACCAGGTTTC TGTTTACATA GTGGCAGTAT TAGAATACAT TTCTGCAGAT 
ATTTTAAAGC TGGTTGGAAA TTATGTACGG AATATACGAC ATTATGAAAT TACAAAACAG 
GACATCAAAG TAGCAATGTG TGCTGATAAG GTATTGATGG ATATGTTTCA TCAAGATGTA 
GAAGATATAA ATATATTATC TTTAACTGAT GAAGAACCTT CCACTTCAGG GGAACAAACG 
TACTATGACT TGGTAAAAGG ATTCATGGCT GAAGTTCGAC AATATATAAG GGAACTTAAT 
CTCATTATTA AAGTTTTTAG AGAACCATTT GTCTCCAATT CAAAATTATT TTCTTCTCAC 
GATGTAGAAA ATATATTTAG TCGTATAGCA GATGTTCATG AACTCAGTGT AAAATTATTG 
GGCCATATAG AAGACACTGT AGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTTGGT 
AGCTGCTTTG AAGACTTAGC AGAGGAATTG GCATTTGATC CATATGAATC ATATGCTCAA 
GACATATTGC GACCTGGTTT TCATGATCAC TTTCTTAGTC AGTTATCCAA GCCTGGGGCA 
GCCCTCTACT TGCAGTCAAT AGGTGAAGGT TTCAAAGAAG CTGTTCAGTA TGTTTTACCC 
AGGCTACTTC TAGCTCCTGT GTACCACTGT CTACATTACT TTGAACTTTT AAAGCAGTTA 
GAAGAGAAGA GTGAAGACCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACGGCTTTG 
CTTAACCTTC AGAGCAGTAT GGAAAGAATA TGTTCCAAAA GTCTTGCAAA ACGGAGATTG 
AGCGAATCTG CATGTCGATT TTATAGCCAA CAGATGAAGG GAAAACAACT AGCAATAAAG 
AAAATGAATG AGATTCAGAA GAACATTGAT GGGTGGGAGG GAAAAGACAT TGGACAGTGT 
TGCAATGAGT TTATCATGGA AGGAACTCTC ACACGTGTAG GTGCCAAACA TGAGAGACAC 
ATATTTCTCT TTGATGGTCT GATGATTTGC TGTAAATCAA ATCATGGTCA GCCAAGACTT 
CCTGGTGCTA GCAATGCTGA GTATCGTCTC AAAGAGAAGT TTTTTATGCG CAAGGTACAA 
ATCAATGATA AGGATGACAC TAATGAATAC AAACATGCCT TTGAAATAAT CTTAAAAGAT 
GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAGGAGA AAAACAACTG GATGGCAGCA 
CTGATATCAT TACAATATCG TAGTACTCTG GAAAGGATGC TGGATGTAAC AATGTTACAA 
GAGGAGAAGG AAGAGCAGAT GAGGCTTCCA AGTTCAGATC TTTATAGATT TGCAGAACCA 
GATTCTGAAG AAAACATAGT ATTTGAAGAA AACATGCAAC CCAAATCTGG AATTCCAATC 
ATCAAAGCAG GAACCGTTAT CAAACTTATA GAGCGACTCA CCTACCATAT GTATGCTGAT 
CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCTT TCTGCAAGCC TCAAGAGCTA 
CTGAGTCTTC TAATAGAGAG GTTTGAAATT CCAGAGCCAG AGCCAACAGA AGCTGATAAG 
ATAGCTATGG AGAATGGAGA TCAACCCCTG AGTGTGGAAC TAAAACGATT TAGAAAAGAA 
TATATACAAC CCGTACAACT TCGAGTACTA AATGTATGTC GGCATTGGGT AGAACATCAC 
TTCTATGATT TTGAAAGGGA TACAGATCTT TTGCAACGAC TGGAAGAATT TATTGGAACA 
GTAAGGGGCA AAGCAATGAA GAAATGGGTT GAATCTATCA CTAAAATCAT TCAACGGAAA 
AAAATGGCAA GAGACAATGG ACCAGGACAT AATATTACAT TTGAAAGTTC ACCACCTGCA 
GTTGAATGGC ATATAAGCAG ACCTGGACAC ACAGAAACTT TTGATCTGCT CACCTTGCAC 
CCAATAGAAA TTGCTCGACA GCTCACTTTA CTTGAGTCAG ATCTCTACAG AGCTGTACAG 
CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAC 
CTTCTGAAAA TGATTCGCCA CACAACTAAT CTTACTTTGT GGTTTGAAAA GTGTATTGTA 
GAAGCAGAAA ACCTAGAGGA AAGAGTAGCT GTGGTGAGCC GAATAATTGA GATTCTTCAA 
GTCTTTCAAG AACTGAACAA CTTTAATGGT GTACTTGAGG TTGTCAGTGC CATGAATTCA 
TCACCTGTTT ATAGACTGGA CCACACGTTT GAGCAAATTC CAAGTCGCCA AAAGAAAATT 
CTAGAGGAAG CTCATGAACT GAGTGAAGAT CACTATAAAA AATATTTGGC AAAACTCAGG 
TCTATTAATC CACCATGTGT GCCTTTCTTT GGGATTTATT TGACAAATAT CTTGAAAACG 
GAAGAAGGCA ATCCTGAGGT TTTGAAAAGA CATGGAAAAG AACTCATAAA CTTTAGCAAA 
AGAAGAAAAG TTGCAGAAAT AACAGGAGAG ATACAGCAGT ACCAAAATCA ACCATATTGT 
TTGCGAGTAG AATCTGATAT CAAAAGATTC TTTGAAAATT TGAATCCAAT GGGAAATAGC 
ATGGAAAAAG AATTCACAGA TTATCTTTTC AACAAGTCAC TGGAAATTGA ACCAAGAAAC 
CCTAAGCCTC TACCGAGATT TCCAAAAAAA TATAGTTATC CCCTCAAGTC TCCTGGTGTT 
CGTCCATCAA ACCCAAGACC AGGTACCATG AGACACCCCA CACCACTGCA GCAGGAACCA 
AGGAAGATCA GTTACAGCCG CATCCCTGAA AGTGAAACAG AAAGTGCAGC ATCAGCACCT 
AATTCTCCAC GGACCCCGTT AACACCTCCT CCAGCTTCTG GTGCTTCCAG TACCACTGAT 
GTGGGCAGCG TGTTTGATTC TGAGCATTCA AGCCCTTTTC ACTCAAGATC TGCTTCAGTA 
TCCTCAATAA ATTTAACTAA GAGCATTGAT GAAATGCCCA TCCCCCCACC AGTGCCCCCA 
AGAAGACGAC CAGAATCCGC TCCAGCAGAA TCTTCTCCCT CAAAGATTAT GCCCAAACAT 
TTGGATAGTC CTCCAGCAAT TCCTCCTCGG CAACCCACAT CAAAAGTCTA TTCACCACGT 
TACTCCGACC GGACCTCGAT CTCTGATCCT CCAGAAAGCC CTCCCCTTTT ACCACCACGA 
GAACCTGTGA GGACACCTGA TGTTTTCTCC AGCTCACCAC TACATCTCCA GCCACCCCCT 
TTGGGCAAAA AAAGTGAACA TGGCAATACT TTTTTCCCAA ACAGCCCCTC CCCCTTTACC 
CCACCACCTC CTCAAACACC TTCTCCTCAC GGCACAAGGA GGCACCTACC ATCACCACCA 
TTAACACAAG ATGTGGACCT TCATTCCATC CCTGCGCCTC CTGTTCCTCC ACGGCAAAGC 
ACTTCTCAAC ACATCCCAAA ACTTCCTCCA AAAACTTATA AAAGGGAACA CACACACCCT 
TCCATGCATA GAGATGGACC ACCTTTGTTG GAGAATGCCC ATTCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003758384.1
CDS	31..3981
Translation	MQALQLQYEFFSEENAPKWRGLLVTALKKVQMQVHPTLASNEDALQYVEELILQLLSMLCQAQPRSVLDVEDRVQKSFPHPIDKWAIADAQSANEKRKRRNPLSLPVEKIHPLLKEVLGYKIDHQVSVYIVAVLEYISADILKLVGNYVRNIRHYEITKQDIKVAMCADKVLMDMFHQDVEDINILSLTDEEPSTSGEQTYYDLVKGFMAEVRQYIRELNLIIKVFREPFVSNSKLFSSHDVENIFSRIADVHELSVKLLGHIEDTVEMTDEGSPHPLVGSCFEDLAEELAFDPYESYAQDILRPGFHDHFLSQLSKPGAALYLQSIGEGFKEAVQYVLPRLLLAPVYHCLHYFELLKQLEEKSEDQEDKECLKQAITALLNLQSSMERICSKSLAKRRLSESACRFYSQQMKGKQLAIKKMNEIQKNIDGWEGKDIGQCCNEFIMEGTLTRVGAKHERHIFLFDGLMICCKSNHGQPRLPGASNAEYRLKEKFFMRKVQINDKDDTNEYKHAFEIILKDENSVIFSAKSAEEKNNWMAALISLQYRSTLERMLDVTMLQEEKEEQMRLPSSDLYRFAEPDSEENIVFEENMQPKSGIPIIKAGTVIKLIERLTYHMYADPNFVRTFLTTYRSFCKPQELLSLLIERFEIPEPEPTEADKIAMENGDQPLSVELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDTDLLQRLEEFIGTVRGKAMKKWVESITKIIQRKKMARDNGPGHNITFESSPPAVEWHISRPGHTETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIVEAENLEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHELSEDHYKKYLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLKRHGKELINFSKRRKVAEITGEIQQYQNQPYCLRVESDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRNPKPLPRFPKKYSYPLKSPGVRPSNPRPGTMRHPTPLQQEPRKISYSRIPESETESAASAPNSPRTPLTPPPASGASSTTDVGSVFDSEHSSPFHSRSASVSSINLTKSIDEMPIPPPVPPRRRPESAPAESSPSKIMPKHLDSPPAIPPRQPTSKVYSPRYSDRTSISDPPESPPLLPPREPVRTPDVFSSSPLHLQPPPLGKKSEHGNTFFPNSPSPFTPPPPQTPSPHGTRRHLPSPPLTQDVDLHSIPAPPVPPRQSTSQHIPKLPPKTYKREHTHPSMHRDGPPLLENAHSS

Target ORF information:

RefSeq Version	XM_003758336.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003758336.3

ORF Insert Sequence:

ATGCAGGCGC TGCAGCTCCA GTACGAGTTC TTCAGCGAGG AAAACGCGCC CAAGTGGAGG 
GGGCTGCTGG TGACGGCCCT GAAAAAGGTC CAGATGCAAG TCCATCCTAC ACTTGCTTCA 
AATGAGGATG CTCTCCAGTA TGTTGAAGAG TTAATTTTAC AGTTGTTAAG TATGCTGTGC 
CAAGCTCAAC CCCGAAGCGT TTTAGATGTT GAGGATCGTG TACAAAAAAG TTTTCCTCAT 
CCAATTGATA AGTGGGCGAT AGCTGATGCT CAATCCGCTA ATGAAAAGAG GAAGCGAAGA 
AATCCATTAT CTCTCCCAGT AGAAAAAATT CACCCTTTAT TAAAGGAAGT TCTAGGATAT 
AAAATTGACC ACCAGGTTTC TGTTTACATA GTGGCAGTAT TAGAATACAT TTCTGCAGAT 
ATTTTAAAGC TGGTTGGAAA TTATGTACGG AATATACGAC ATTATGAAAT TACAAAACAG 
GACATCAAAG TAGCAATGTG TGCTGATAAG GTATTGATGG ATATGTTTCA TCAAGATGTA 
GAAGATATAA ATATATTATC TTTAACTGAT GAAGAACCTT CCACTTCAGG GGAACAAACG 
TACTATGACT TGGTAAAAGG ATTCATGGCT GAAGTTCGAC AATATATAAG GGAACTTAAT 
CTCATTATTA AAGTTTTTAG AGAACCATTT GTCTCCAATT CAAAATTATT TTCTTCTCAC 
GATGTAGAAA ATATATTTAG TCGTATAGCA GATGTTCATG AACTCAGTGT AAAATTATTG 
GGCCATATAG AAGACACTGT AGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTTGGT 
AGCTGCTTTG AAGACTTAGC AGAGGAATTG GCATTTGATC CATATGAATC ATATGCTCAA 
GACATATTGC GACCTGGTTT TCATGATCAC TTTCTTAGTC AGTTATCCAA GCCTGGGGCA 
GCCCTCTACT TGCAGTCAAT AGGTGAAGGT TTCAAAGAAG CTGTTCAGTA TGTTTTACCC 
AGGCTACTTC TAGCTCCTGT GTACCACTGT CTACATTACT TTGAACTTTT AAAGCAGTTA 
GAAGAGAAGA GTGAAGACCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACGGCTTTG 
CTTAACCTTC AGAGCAGTAT GGAAAGAATA TGTTCCAAAA GTCTTGCAAA ACGGAGATTG 
AGCGAATCTG CATGTCGATT TTATAGCCAA CAGATGAAGG GAAAACAACT AGCAATAAAG 
AAAATGAATG AGATTCAGAA GAACATTGAT GGGTGGGAGG GAAAAGACAT TGGACAGTGT 
TGCAATGAGT TTATCATGGA AGGAACTCTC ACACGTGTAG GTGCCAAACA TGAGAGACAC 
ATATTTCTCT TTGATGGTCT GATGATTTGC TGTAAATCAA ATCATGGTCA GCCAAGACTT 
CCTGGTGCTA GCAATGCTGA GTATCGTCTC AAAGAGAAGT TTTTTATGCG CAAGGTACAA 
ATCAATGATA AGGATGACAC TAATGAATAC AAACATGCCT TTGAAATAAT CTTAAAAGAT 
GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAGGAGA AAAACAACTG GATGGCAGCA 
CTGATATCAT TACAATATCG TAGTACTCTG GAAAGGATGC TGGATGTAAC AATGTTACAA 
GAGGAGAAGG AAGAGCAGAT GAGGCTTCCA AGTTCAGATC TTTATAGATT TGCAGAACCA 
GATTCTGAAG AAAACATAGT ATTTGAAGAA AACATGCAAC CCAAATCTGG AATTCCAATC 
ATCAAAGCAG GAACCGTTAT CAAACTTATA GAGCGACTCA CCTACCATAT GTATGCTGAT 
CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCTT TCTGCAAGCC TCAAGAGCTA 
CTGAGTCTTC TAATAGAGAG GTTTGAAATT CCAGAGCCAG AGCCAACAGA AGCTGATAAG 
ATAGCTATGG AGAATGGAGA TCAACCCCTG AGTGTGGAAC TAAAACGATT TAGAAAAGAA 
TATATACAAC CCGTACAACT TCGAGTACTA AATGTATGTC GGCATTGGGT AGAACATCAC 
TTCTATGATT TTGAAAGGGA TACAGATCTT TTGCAACGAC TGGAAGAATT TATTGGAACA 
GTAAGGGGCA AAGCAATGAA GAAATGGGTT GAATCTATCA CTAAAATCAT TCAACGGAAA 
AAAATGGCAA GAGACAATGG ACCAGGACAT AATATTACAT TTGAAAGTTC ACCACCTGCA 
GTTGAATGGC ATATAAGCAG ACCTGGACAC ACAGAAACTT TTGATCTGCT CACCTTGCAC 
CCAATAGAAA TTGCTCGACA GCTCACTTTA CTTGAGTCAG ATCTCTACAG AGCTGTACAG 
CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAC 
CTTCTGAAAA TGATTCGCCA CACAACTAAT CTTACTTTGT GGTTTGAAAA GTGTATTGTA 
GAAGCAGAAA ACCTAGAGGA AAGAGTAGCT GTGGTGAGCC GAATAATTGA GATTCTTCAA 
GTCTTTCAAG AACTGAACAA CTTTAATGGT GTACTTGAGG TTGTCAGTGC CATGAATTCA 
TCACCTGTTT ATAGACTGGA CCACACGTTT GAGCAAATTC CAAGTCGCCA AAAGAAAATT 
CTAGAGGAAG CTCATGAACT GAGTGAAGAT CACTATAAAA AATATTTGGC AAAACTCAGG 
TCTATTAATC CACCATGTGT GCCTTTCTTT GGGATTTATT TGACAAATAT CTTGAAAACG 
GAAGAAGGCA ATCCTGAGGT TTTGAAAAGA CATGGAAAAG AACTCATAAA CTTTAGCAAA 
AGAAGAAAAG TTGCAGAAAT AACAGGAGAG ATACAGCAGT ACCAAAATCA ACCATATTGT 
TTGCGAGTAG AATCTGATAT CAAAAGATTC TTTGAAAATT TGAATCCAAT GGGAAATAGC 
ATGGAAAAAG AATTCACAGA TTATCTTTTC AACAAGTCAC TGGAAATTGA ACCAAGAAAC 
CCTAAGCCTC TACCGAGATT TCCAAAAAAA TATAGTTATC CCCTCAAGTC TCCTGGTGTT 
CGTCCATCAA ACCCAAGACC AGGTACCATG AGACACCCCA CACCACTGCA GCAGGAACCA 
AGGAAGATCA GTTACAGCCG CATCCCTGAA AGTGAAACAG AAAGTGCAGC ATCAGCACCT 
AATTCTCCAC GGACCCCGTT AACACCTCCT CCAGCTTCTG GTGCTTCCAG TACCACTGAT 
GTGGGCAGCG TGTTTGATTC TGAGCATTCA AGCCCTTTTC ACTCAAGATC TGCTTCAGTA 
TCCTCAATAA ATTTAACTAA GAGCATTGAT GAAATGCCCA TCCCCCCACC AGTGCCCCCA 
AGAAGACGAC CAGAATCCGC TCCAGCAGAA TCTTCTCCCT CAAAGATTAT GCCCAAACAT 
TTGGATAGTC CTCCAGCAAT TCCTCCTCGG CAACCCACAT CAAAAGTCTA TTCACCACGT 
TACTCCGACC GGACCTCGAT CTCTGATCCT CCAGAAAGCC CTCCCCTTTT ACCACCACGA 
GAACCTGTGA GGACACCTGA TGTTTTCTCC AGCTCACCAC TACATCTCCA GCCACCCCCT 
TTGGGCAAAA AAAGTGAACA TGGCAATACT TTTTTCCCAA ACAGCCCCTC CCCCTTTACC 
CCACCACCTC CTCAAACACC TTCTCCTCAC GGCACAAGGA GGCACCTACC ATCACCACCA 
TTAACACAAG ATGTGGACCT TCATTCCATC CCTGCGCCTC CTGTTCCTCC ACGGCAAAGC 
ACTTCTCAAC ACATCCCAAA ACTTCCTCCA AAAACTTATA AAAGGGAACA CACACACCCT 
TCCATGCATA GAGATGGACC ACCTTTGTTG GAGAATGCCC ATTCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SOS1 ( XM_003758336.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003758384.1 Sarcophilus harrisii son of sevenless homolog 1 (Drosophila) (SOS1), transcript variant X2, mRNA.

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CloneID	OSa120073
Clone ID Related Accession (Same CDS sequence)	XM_003758336.4 , XM_003758336.3 , XM_003758336.2
Accession Version	XM_003758336.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	son of sevenless homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816756.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_003758336.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC TGCAGCTCCA GTACGAGTTC TTCAGCGAGG AAAACGCGCC CAAGTGGAGG 
GGGCTGCTGG TGACGGCCCT GAAAAAGGTC CAGATGCAAG TCCATCCTAC ACTTGCTTCA 
AATGAGGATG CTCTCCAGTA TGTTGAAGAG TTAATTTTAC AGTTGTTAAG TATGCTGTGC 
CAAGCTCAAC CCCGAAGCGT TTTAGATGTT GAGGATCGTG TACAAAAAAG TTTTCCTCAT 
CCAATTGATA AGTGGGCGAT AGCTGATGCT CAATCCGCTA ATGAAAAGAG GAAGCGAAGA 
AATCCATTAT CTCTCCCAGT AGAAAAAATT CACCCTTTAT TAAAGGAAGT TCTAGGATAT 
AAAATTGACC ACCAGGTTTC TGTTTACATA GTGGCAGTAT TAGAATACAT TTCTGCAGAT 
ATTTTAAAGC TGGTTGGAAA TTATGTACGG AATATACGAC ATTATGAAAT TACAAAACAG 
GACATCAAAG TAGCAATGTG TGCTGATAAG GTATTGATGG ATATGTTTCA TCAAGATGTA 
GAAGATATAA ATATATTATC TTTAACTGAT GAAGAACCTT CCACTTCAGG GGAACAAACG 
TACTATGACT TGGTAAAAGG ATTCATGGCT GAAGTTCGAC AATATATAAG GGAACTTAAT 
CTCATTATTA AAGTTTTTAG AGAACCATTT GTCTCCAATT CAAAATTATT TTCTTCTCAC 
GATGTAGAAA ATATATTTAG TCGTATAGCA GATGTTCATG AACTCAGTGT AAAATTATTG 
GGCCATATAG AAGACACTGT AGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTTGGT 
AGCTGCTTTG AAGACTTAGC AGAGGAATTG GCATTTGATC CATATGAATC ATATGCTCAA 
GACATATTGC GACCTGGTTT TCATGATCAC TTTCTTAGTC AGTTATCCAA GCCTGGGGCA 
GCCCTCTACT TGCAGTCAAT AGGTGAAGGT TTCAAAGAAG CTGTTCAGTA TGTTTTACCC 
AGGCTACTTC TAGCTCCTGT GTACCACTGT CTACATTACT TTGAACTTTT AAAGCAGTTA 
GAAGAGAAGA GTGAAGACCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACGGCTTTG 
CTTAACCTTC AGAGCAGTAT GGAAAGAATA TGTTCCAAAA GTCTTGCAAA ACGGAGATTG 
AGCGAATCTG CATGTCGATT TTATAGCCAA CAGATGAAGG GAAAACAACT AGCAATAAAG 
AAAATGAATG AGATTCAGAA GAACATTGAT GGGTGGGAGG GAAAAGACAT TGGACAGTGT 
TGCAATGAGT TTATCATGGA AGGAACTCTC ACACGTGTAG GTGCCAAACA TGAGAGACAC 
ATATTTCTCT TTGATGGTCT GATGATTTGC TGTAAATCAA ATCATGGTCA GCCAAGACTT 
CCTGGTGCTA GCAATGCTGA GTATCGTCTC AAAGAGAAGT TTTTTATGCG CAAGGTACAA 
ATCAATGATA AGGATGACAC TAATGAATAC AAACATGCCT TTGAAATAAT CTTAAAAGAT 
GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAGGAGA AAAACAACTG GATGGCAGCA 
CTGATATCAT TACAATATCG TAGTACTCTG GAAAGGATGC TGGATGTAAC AATGTTACAA 
GAGGAGAAGG AAGAGCAGAT GAGGCTTCCA AGTTCAGATC TTTATAGATT TGCAGAACCA 
GATTCTGAAG AAAACATAGT ATTTGAAGAA AACATGCAAC CCAAATCTGG AATTCCAATC 
ATCAAAGCAG GAACCGTTAT CAAACTTATA GAGCGACTCA CCTACCATAT GTATGCTGAT 
CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCTT TCTGCAAGCC TCAAGAGCTA 
CTGAGTCTTC TAATAGAGAG GTTTGAAATT CCAGAGCCAG AGCCAACAGA AGCTGATAAG 
ATAGCTATGG AGAATGGAGA TCAACCCCTG AGTGTGGAAC TAAAACGATT TAGAAAAGAA 
TATATACAAC CCGTACAACT TCGAGTACTA AATGTATGTC GGCATTGGGT AGAACATCAC 
TTCTATGATT TTGAAAGGGA TACAGATCTT TTGCAACGAC TGGAAGAATT TATTGGAACA 
GTAAGGGGCA AAGCAATGAA GAAATGGGTT GAATCTATCA CTAAAATCAT TCAACGGAAA 
AAAATGGCAA GAGACAATGG ACCAGGACAT AATATTACAT TTGAAAGTTC ACCACCTGCA 
GTTGAATGGC ATATAAGCAG ACCTGGACAC ACAGAAACTT TTGATCTGCT CACCTTGCAC 
CCAATAGAAA TTGCTCGACA GCTCACTTTA CTTGAGTCAG ATCTCTACAG AGCTGTACAG 
CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAC 
CTTCTGAAAA TGATTCGCCA CACAACTAAT CTTACTTTGT GGTTTGAAAA GTGTATTGTA 
GAAGCAGAAA ACCTAGAGGA AAGAGTAGCT GTGGTGAGCC GAATAATTGA GATTCTTCAA 
GTCTTTCAAG AACTGAACAA CTTTAATGGT GTACTTGAGG TTGTCAGTGC CATGAATTCA 
TCACCTGTTT ATAGACTGGA CCACACGTTT GAGCAAATTC CAAGTCGCCA AAAGAAAATT 
CTAGAGGAAG CTCATGAACT GAGTGAAGAT CACTATAAAA AATATTTGGC AAAACTCAGG 
TCTATTAATC CACCATGTGT GCCTTTCTTT GGGATTTATT TGACAAATAT CTTGAAAACG 
GAAGAAGGCA ATCCTGAGGT TTTGAAAAGA CATGGAAAAG AACTCATAAA CTTTAGCAAA 
AGAAGAAAAG TTGCAGAAAT AACAGGAGAG ATACAGCAGT ACCAAAATCA ACCATATTGT 
TTGCGAGTAG AATCTGATAT CAAAAGATTC TTTGAAAATT TGAATCCAAT GGGAAATAGC 
ATGGAAAAAG AATTCACAGA TTATCTTTTC AACAAGTCAC TGGAAATTGA ACCAAGAAAC 
CCTAAGCCTC TACCGAGATT TCCAAAAAAA TATAGTTATC CCCTCAAGTC TCCTGGTGTT 
CGTCCATCAA ACCCAAGACC AGGTACCATG AGACACCCCA CACCACTGCA GCAGGAACCA 
AGGAAGATCA GTTACAGCCG CATCCCTGAA AGTGAAACAG AAAGTGCAGC ATCAGCACCT 
AATTCTCCAC GGACCCCGTT AACACCTCCT CCAGCTTCTG GTGCTTCCAG TACCACTGAT 
GTGGGCAGCG TGTTTGATTC TGAGCATTCA AGCCCTTTTC ACTCAAGATC TGCTTCAGTA 
TCCTCAATAA ATTTAACTAA GAGCATTGAT GAAATGCCCA TCCCCCCACC AGTGCCCCCA 
AGAAGACGAC CAGAATCCGC TCCAGCAGAA TCTTCTCCCT CAAAGATTAT GCCCAAACAT 
TTGGATAGTC CTCCAGCAAT TCCTCCTCGG CAACCCACAT CAAAAGTCTA TTCACCACGT 
TACTCCGACC GGACCTCGAT CTCTGATCCT CCAGAAAGCC CTCCCCTTTT ACCACCACGA 
GAACCTGTGA GGACACCTGA TGTTTTCTCC AGCTCACCAC TACATCTCCA GCCACCCCCT 
TTGGGCAAAA AAAGTGAACA TGGCAATACT TTTTTCCCAA ACAGCCCCTC CCCCTTTACC 
CCACCACCTC CTCAAACACC TTCTCCTCAC GGCACAAGGA GGCACCTACC ATCACCACCA 
TTAACACAAG ATGTGGACCT TCATTCCATC CCTGCGCCTC CTGTTCCTCC ACGGCAAAGC 
ACTTCTCAAC ACATCCCAAA ACTTCCTCCA AAAACTTATA AAAGGGAACA CACACACCCT 
TCCATGCATA GAGATGGACC ACCTTTGTTG GAGAATGCCC ATTCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003758384.1
CDS	1..3951
Translation	MQALQLQYEFFSEENAPKWRGLLVTALKKVQMQVHPTLASNEDALQYVEELILQLLSMLCQAQPRSVLDVEDRVQKSFPHPIDKWAIADAQSANEKRKRRNPLSLPVEKIHPLLKEVLGYKIDHQVSVYIVAVLEYISADILKLVGNYVRNIRHYEITKQDIKVAMCADKVLMDMFHQDVEDINILSLTDEEPSTSGEQTYYDLVKGFMAEVRQYIRELNLIIKVFREPFVSNSKLFSSHDVENIFSRIADVHELSVKLLGHIEDTVEMTDEGSPHPLVGSCFEDLAEELAFDPYESYAQDILRPGFHDHFLSQLSKPGAALYLQSIGEGFKEAVQYVLPRLLLAPVYHCLHYFELLKQLEEKSEDQEDKECLKQAITALLNLQSSMERICSKSLAKRRLSESACRFYSQQMKGKQLAIKKMNEIQKNIDGWEGKDIGQCCNEFIMEGTLTRVGAKHERHIFLFDGLMICCKSNHGQPRLPGASNAEYRLKEKFFMRKVQINDKDDTNEYKHAFEIILKDENSVIFSAKSAEEKNNWMAALISLQYRSTLERMLDVTMLQEEKEEQMRLPSSDLYRFAEPDSEENIVFEENMQPKSGIPIIKAGTVIKLIERLTYHMYADPNFVRTFLTTYRSFCKPQELLSLLIERFEIPEPEPTEADKIAMENGDQPLSVELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDTDLLQRLEEFIGTVRGKAMKKWVESITKIIQRKKMARDNGPGHNITFESSPPAVEWHISRPGHTETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIVEAENLEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHELSEDHYKKYLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLKRHGKELINFSKRRKVAEITGEIQQYQNQPYCLRVESDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRNPKPLPRFPKKYSYPLKSPGVRPSNPRPGTMRHPTPLQQEPRKISYSRIPESETESAASAPNSPRTPLTPPPASGASSTTDVGSVFDSEHSSPFHSRSASVSSINLTKSIDEMPIPPPVPPRRRPESAPAESSPSKIMPKHLDSPPAIPPRQPTSKVYSPRYSDRTSISDPPESPPLLPPREPVRTPDVFSSSPLHLQPPPLGKKSEHGNTFFPNSPSPFTPPPPQTPSPHGTRRHLPSPPLTQDVDLHSIPAPPVPPRQSTSQHIPKLPPKTYKREHTHPSMHRDGPPLLENAHSS

Target ORF information:

RefSeq Version	XM_003758336.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii son of sevenless homolog 1 (Drosophila) (SOS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003758336.2

ORF Insert Sequence:

ATGCAGGCGC TGCAGCTCCA GTACGAGTTC TTCAGCGAGG AAAACGCGCC CAAGTGGAGG 
GGGCTGCTGG TGACGGCCCT GAAAAAGGTC CAGATGCAAG TCCATCCTAC ACTTGCTTCA 
AATGAGGATG CTCTCCAGTA TGTTGAAGAG TTAATTTTAC AGTTGTTAAG TATGCTGTGC 
CAAGCTCAAC CCCGAAGCGT TTTAGATGTT GAGGATCGTG TACAAAAAAG TTTTCCTCAT 
CCAATTGATA AGTGGGCGAT AGCTGATGCT CAATCCGCTA ATGAAAAGAG GAAGCGAAGA 
AATCCATTAT CTCTCCCAGT AGAAAAAATT CACCCTTTAT TAAAGGAAGT TCTAGGATAT 
AAAATTGACC ACCAGGTTTC TGTTTACATA GTGGCAGTAT TAGAATACAT TTCTGCAGAT 
ATTTTAAAGC TGGTTGGAAA TTATGTACGG AATATACGAC ATTATGAAAT TACAAAACAG 
GACATCAAAG TAGCAATGTG TGCTGATAAG GTATTGATGG ATATGTTTCA TCAAGATGTA 
GAAGATATAA ATATATTATC TTTAACTGAT GAAGAACCTT CCACTTCAGG GGAACAAACG 
TACTATGACT TGGTAAAAGG ATTCATGGCT GAAGTTCGAC AATATATAAG GGAACTTAAT 
CTCATTATTA AAGTTTTTAG AGAACCATTT GTCTCCAATT CAAAATTATT TTCTTCTCAC 
GATGTAGAAA ATATATTTAG TCGTATAGCA GATGTTCATG AACTCAGTGT AAAATTATTG 
GGCCATATAG AAGACACTGT AGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTTGGT 
AGCTGCTTTG AAGACTTAGC AGAGGAATTG GCATTTGATC CATATGAATC ATATGCTCAA 
GACATATTGC GACCTGGTTT TCATGATCAC TTTCTTAGTC AGTTATCCAA GCCTGGGGCA 
GCCCTCTACT TGCAGTCAAT AGGTGAAGGT TTCAAAGAAG CTGTTCAGTA TGTTTTACCC 
AGGCTACTTC TAGCTCCTGT GTACCACTGT CTACATTACT TTGAACTTTT AAAGCAGTTA 
GAAGAGAAGA GTGAAGACCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACGGCTTTG 
CTTAACCTTC AGAGCAGTAT GGAAAGAATA TGTTCCAAAA GTCTTGCAAA ACGGAGATTG 
AGCGAATCTG CATGTCGATT TTATAGCCAA CAGATGAAGG GAAAACAACT AGCAATAAAG 
AAAATGAATG AGATTCAGAA GAACATTGAT GGGTGGGAGG GAAAAGACAT TGGACAGTGT 
TGCAATGAGT TTATCATGGA AGGAACTCTC ACACGTGTAG GTGCCAAACA TGAGAGACAC 
ATATTTCTCT TTGATGGTCT GATGATTTGC TGTAAATCAA ATCATGGTCA GCCAAGACTT 
CCTGGTGCTA GCAATGCTGA GTATCGTCTC AAAGAGAAGT TTTTTATGCG CAAGGTACAA 
ATCAATGATA AGGATGACAC TAATGAATAC AAACATGCCT TTGAAATAAT CTTAAAAGAT 
GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAGGAGA AAAACAACTG GATGGCAGCA 
CTGATATCAT TACAATATCG TAGTACTCTG GAAAGGATGC TGGATGTAAC AATGTTACAA 
GAGGAGAAGG AAGAGCAGAT GAGGCTTCCA AGTTCAGATC TTTATAGATT TGCAGAACCA 
GATTCTGAAG AAAACATAGT ATTTGAAGAA AACATGCAAC CCAAATCTGG AATTCCAATC 
ATCAAAGCAG GAACCGTTAT CAAACTTATA GAGCGACTCA CCTACCATAT GTATGCTGAT 
CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCTT TCTGCAAGCC TCAAGAGCTA 
CTGAGTCTTC TAATAGAGAG GTTTGAAATT CCAGAGCCAG AGCCAACAGA AGCTGATAAG 
ATAGCTATGG AGAATGGAGA TCAACCCCTG AGTGTGGAAC TAAAACGATT TAGAAAAGAA 
TATATACAAC CCGTACAACT TCGAGTACTA AATGTATGTC GGCATTGGGT AGAACATCAC 
TTCTATGATT TTGAAAGGGA TACAGATCTT TTGCAACGAC TGGAAGAATT TATTGGAACA 
GTAAGGGGCA AAGCAATGAA GAAATGGGTT GAATCTATCA CTAAAATCAT TCAACGGAAA 
AAAATGGCAA GAGACAATGG ACCAGGACAT AATATTACAT TTGAAAGTTC ACCACCTGCA 
GTTGAATGGC ATATAAGCAG ACCTGGACAC ACAGAAACTT TTGATCTGCT CACCTTGCAC 
CCAATAGAAA TTGCTCGACA GCTCACTTTA CTTGAGTCAG ATCTCTACAG AGCTGTACAG 
CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAC 
CTTCTGAAAA TGATTCGCCA CACAACTAAT CTTACTTTGT GGTTTGAAAA GTGTATTGTA 
GAAGCAGAAA ACCTAGAGGA AAGAGTAGCT GTGGTGAGCC GAATAATTGA GATTCTTCAA 
GTCTTTCAAG AACTGAACAA CTTTAATGGT GTACTTGAGG TTGTCAGTGC CATGAATTCA 
TCACCTGTTT ATAGACTGGA CCACACGTTT GAGCAAATTC CAAGTCGCCA AAAGAAAATT 
CTAGAGGAAG CTCATGAACT GAGTGAAGAT CACTATAAAA AATATTTGGC AAAACTCAGG 
TCTATTAATC CACCATGTGT GCCTTTCTTT GGGATTTATT TGACAAATAT CTTGAAAACG 
GAAGAAGGCA ATCCTGAGGT TTTGAAAAGA CATGGAAAAG AACTCATAAA CTTTAGCAAA 
AGAAGAAAAG TTGCAGAAAT AACAGGAGAG ATACAGCAGT ACCAAAATCA ACCATATTGT 
TTGCGAGTAG AATCTGATAT CAAAAGATTC TTTGAAAATT TGAATCCAAT GGGAAATAGC 
ATGGAAAAAG AATTCACAGA TTATCTTTTC AACAAGTCAC TGGAAATTGA ACCAAGAAAC 
CCTAAGCCTC TACCGAGATT TCCAAAAAAA TATAGTTATC CCCTCAAGTC TCCTGGTGTT 
CGTCCATCAA ACCCAAGACC AGGTACCATG AGACACCCCA CACCACTGCA GCAGGAACCA 
AGGAAGATCA GTTACAGCCG CATCCCTGAA AGTGAAACAG AAAGTGCAGC ATCAGCACCT 
AATTCTCCAC GGACCCCGTT AACACCTCCT CCAGCTTCTG GTGCTTCCAG TACCACTGAT 
GTGGGCAGCG TGTTTGATTC TGAGCATTCA AGCCCTTTTC ACTCAAGATC TGCTTCAGTA 
TCCTCAATAA ATTTAACTAA GAGCATTGAT GAAATGCCCA TCCCCCCACC AGTGCCCCCA 
AGAAGACGAC CAGAATCCGC TCCAGCAGAA TCTTCTCCCT CAAAGATTAT GCCCAAACAT 
TTGGATAGTC CTCCAGCAAT TCCTCCTCGG CAACCCACAT CAAAAGTCTA TTCACCACGT 
TACTCCGACC GGACCTCGAT CTCTGATCCT CCAGAAAGCC CTCCCCTTTT ACCACCACGA 
GAACCTGTGA GGACACCTGA TGTTTTCTCC AGCTCACCAC TACATCTCCA GCCACCCCCT 
TTGGGCAAAA AAAGTGAACA TGGCAATACT TTTTTCCCAA ACAGCCCCTC CCCCTTTACC 
CCACCACCTC CTCAAACACC TTCTCCTCAC GGCACAAGGA GGCACCTACC ATCACCACCA 
TTAACACAAG ATGTGGACCT TCATTCCATC CCTGCGCCTC CTGTTCCTCC ACGGCAAAGC 
ACTTCTCAAC ACATCCCAAA ACTTCCTCCA AAAACTTATA AAAGGGAACA CACACACCCT 
TCCATGCATA GAGATGGACC ACCTTTGTTG GAGAATGCCC ATTCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SOS1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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