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AAV ITR Guarantee Service

The integrity of ITR sequences is essential for efficient viral packaging and is crucial for clinical trials and therapy approval, yet preserving these regions during plasmid DNA preparation is often challenging.

At GenScript, we overcome this challenge with our AAV ITR Guarantee Service. With over 20 years of expertise, we combine a specialized ITR plasmid DNA preparation protocol with proprietary ITR Sanger sequencing technology to ensure the highest accuracy and integrity of your ITR sequences. Bundle our AAV ITR Guarantee Service with your molecular biology projects today and ensure 100% ITR sequence integrity, enabling worry-free downstream applications.

ITR-Perfect Plasmids, Delivered to You

Over 20 years of Expertise Synthesizing AAV Plasmids

Over 20 years of Expertise Synthesizing AAV Plasmids

Proprietary Sanger Sequencing Method Ensuring Accurate Results

Proprietary Sanger Sequencing Method Ensuring Accurate Results

Dedicated Production Process with Proprietary GenStable™ ITR Strain

Dedicated Production Process with Proprietary GenStable™ ITR Strain

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Molecular Biology

How to Order & Service Specifications

Quality controls Proprietary ITR sanger sequencing to ensure 100% ITR sequence integrity
Available add-ons
  • Restriction enzyme analysis
  • Proprietary ITR nanopore sequencing

Specialized ITR Sanger Sequencing Ensures Accurate Results

Conventional sanger sequencing often struggles to read through ITR regions because of their high GC content and repetitive nature, causing the sequencing reaction to terminate prematurely and leading to incomplete results. GenScript's ITR Sanger Sequencing technology overcomes this challenge, to ensure accurate and reliable sequencing of ITRs with clear, accurate peaks.

GenStable™ ITR Strain Ensures ITR Plasmid Integrity

pUC57-GFP plasmid containing wildtype AAV2 ITRs was constructed and subcultured into GenStable™ ITR and NEB Stable strains over 10 generations. SmaI digestion and Nanopore Sequencing was conducted on even-numbered generations to evaluate ITR sequence integrity. The GenStable™ ITR strain demonstrated a significantly superior ability to maintain ITR sequence integrity during plasmid DNA preparation.

GenScript's GenStable™ Host Strains

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Nanopore Sequencing Gives You Full Insight of Your ITR Purity

Experience unmatched precision with GenScript's proprietary nanopore sequencing technology, designed to give additional insights of your ITR regions. Our comprehensive deliverables include detailed information such as data volume, average read length, sequencing depth, coverage, results, and verified ITR purity.

Integrity
Plasmid A 97.4%
Plasmid B 53.1%

Relative Services

AAV Plasmid Solutions

Empowering your research with reliable AAV plasmids

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AAV Applied Vector

Build-In ITR vectors with unmatched stability

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GenStable™ Host Strains

Engineered specifically for stable, high-yield production of challenging plasmids.

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Off-the-Shelf Plasmids

Next-day shipping of most popular plasmids

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With GenScript AAV ITR Guarantee Service

Get in Touch With GenScript AAV ITR Guarantee Service
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