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GenScript’s Plasmid Linearization Service provides mRNA researchers with unparalleled expertise, delivering consistent and superior quality results every time. With our rigorous quality control measures, we guarantee linearized plasmids of the highest purity, ensuring efficiency and scalability for a seamless in vitro transcription reaction. Streamline your research process with our comprehensive one-stop solution, spanning from gene synthesis to providing a ready-to-use linearized template.
Extensive Quality Controls
Choose from up to 15 QC options
Scalability
µg to g level
Animal Free Production, endotoxin controls for your mRNA downstream applications
| For Cloning/Transfection Use | For Animal Study Use | |
|---|---|---|
| Price | Starting from $89 | IQR |
| Quantity | > 30 µg | > 5 mg |
| Default Quality Control | ||
| Endotoxin Level | <10 EU/mg (based on plasmid prep grade) | <10 EU/mg |
| Linearization Purification |
|
|
| Linearization Confirmation | Agarose gel electrophoresis (single band) | HPLC (≥ 95%) |
| Residual Host gDNA | Agarose gel electrophoresis (< 15% plasmid band) | qPCR (< 5%) |
| Residual Host Cell Protein | N/A | Nano orange < 2% |
| Residual RNA | Agarose gel electrophoresis | Agarose gel electrophoresis |
| For Cloning/Transfection Use | For Animal Study Use | |
|---|---|---|
| Additional Linearization Purification |
|
N/A |
| Advance Endotoxin Removal | <0.005 EU/µg quantitative TAL (default), LAL (optional) | |
| Mycoplasma Detection | N/A | Luminescence-based Detection Kit |
| Kanamycin Detection | N/A | ELISA |
| HPLC (quantitative linearization) | N/A | Additional cost if >98% |
| Animal Free Process with TSE/BSE Certification | RNase, BsaI/EcoRI/BspQI , TSE/BSE certification | |
| Bio-burden Assay | 48 hrs. YPD and LB | |
| Nicked Plasmid Treatment | T4 Ligase Treated Circularization | |
Utilizing pure linearized plasmid is essential for achieving successful IVT reactions that result in elevated mRNA levels. GenScript's Plasmid Linearization ensures meticulous purification steps and rigorous quality controls, providing researchers with the best quality input for optimal mRNA production.
Pure linearized plasmid lead to successful IVT
| Linearized plasmid purity% | Linear plasmid% | IVT yield (μg) |
|---|---|---|
| 50% | 50% | 16.8 |
| 90% | 90% | 18.4 |
| 95% | 95% | 24.7 |
| 98% | 98% | 23.8 |
| >98% | 100% | 24.9 |
Selecting the appropriate, high-quality restriction enzymes is essential for the best IVT reactions and maximum mRNA output. Elements like the length of the enzyme recognition sequence, the nature of the cleavage product, enzyme stability, and overall quality can significantly influence your mRNA product and its yield. At GenScript, our PhD-level scientists are on hand to guide and advise on your enzyme choices and address any experimental uncertainties.
Figure 1. The circular mRNA template encoding eGFP was linearized utilizing BspQI and XbaI Restriction Enzymes respectively. IVT mRNA was subsequently produced from these linearized plasmids and examined for quality and protein expression. 293T cells were transfected with 0.1 µg of the mRNA using the Lipofectamine 2000 reagent (Invitrogen). GFP expression was observed 48 hours after transfection. The data indicates that the mRNA derived from the BspQI-linearized template exhibited roughly 1.9 times higher GFP expression compared to the XbaI version.
100% sequence guarantee
Turnaround time starting from 72hrs
Gene
synthesized up to 200k bp
Basic research to animal study grade
Plasmid DNA from microgram to gram level
Poly(A) and AAV ITR guarantee
Ideal for scientists seeking high-quality large-scale mRNA IVT template
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