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Presence of Chlamydia trachomatis and Mycoplasma spp, but not Neisseria gonorrhoeae and Treponema pallidum, in women undergoing an infertility …

springerlink. 2017; 
Min Li, Xiaomei Zhang, Ke Huang, Haixiang Qiu, Jilei Zhang, Yuan Kang & Chengming Wang
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Gene Synthesis For use as quantitative standards, the products of the Mycoplasma FRET-PCR on vaginal swabs were gel purified using a QIAquick Gel Extraction Kit (Qiagen, Valencia, CA, USA). An aliquot of the purified product was sequenced for confirmation at GenScript (Nanjing Jiangsu, China) and the remainder quantified (ng/ml) with the Quanti-iT™ PicoGreen®dsDNA Assay Kit. After using the molecular mass of the 16S rRNA gene to calculate the molarity of the solution, dilutions were made to give solutions containing 10,000, 1000, 100, 10, and 1 gene copies per reaction. These were amplified by Mycoplasma FRET-PCR in triplicate to determine the detection limit of the PCR. Get A Quote

Abstract

Chlamydia trachomatis, Mycoplasma spp., Neisseria gonorrhoeae and Treponema pallidumare sexually transmitted pathogens that threaten reproductive health worldwide. In this study, vaginal swabs obtained from women (n = 133) that attended an infertility clinic in China were tested with qPCRs for C. trachomatis, Mycoplasma spp., N. gonorrhoeae, T. pallidum and tetracycline resistance genes. While none of vaginal swabs were positive for N. gonorrhoeae and T. pallidum, 18.8% (25/133) of the swabs were positive for Chlamydia spp. and 17.3% of the swabs (23/133) were positive for Mycoplasma species. All swabs tested were positive for tetracycline resistance gene tet(M) which is the most effective ... More

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