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Chloramphenicol acetyltransferase—a new selectable marker in stable nuclear transformation of the red alga Cyanidioschyzon merolae.

Protoplasma.. 2017-01; 
Zienkiewicz M, Krupnik T, Drożak A, Golke A, Romanowska E.
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Gene Synthesis ... possible. Results The sequence of cat gene originated from Tn9 transposon was subjected to a codon-usage optimization, according to codon- usage optimization table, provided by GenScript HK Limited, Hong Kong, China. The ... Get A Quote

Abstract

In this study, we have shown the applicability of chloramphenicol acetyltransferase as a new and convenient selectable marker for stable nuclear transformation as well as potential chloroplast transformation of Cyanidioschyzon merolae-a new model organism, which offers unique opportunities for studding the mitochondrial and plastid physiology as well as various evolutionary, structural, and functional features of the photosynthetic apparatus.

Keywords

Chloramphenicol acetyltransferase (CAT) stable genome transformation; Cyanidioschyzon merolae