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Noggin Fc Chimera, Human

Recombinant Human Noggin Fc Chimera produced in CHO cells is a polypeptide chain containing 438 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhNoggin has a molecular mass of 57 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
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Product Introduction
Species Human
Protein Construction
Noggin (Gln28-Cys232)
Accession # Q13253		 hFc
N-term C-term
Purity > 97% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 60.0 ng/ml, measured in a bioassay using ATDC5 cells in the presence of 10.0 ng/ml human BMP-4.
Expression System CHO
Apparent Molecular Weight ~57 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Background
Target Background Noggin, also known as NOG, is a homodimeric glycoprotein that bindsto and modulates the activity of TGF-beta family ligands. It is expressed in condensing cartilage and immature chondrocytes. Noggin antagonizes bone morphogenetic protein (BMP) activities by blocking epitopes on BMPs needed for binding to their receptors. Noggin has been shown to be involved in many developmental processes, such as neural tube formation and joint formation. During development, Noggin diffuses through extracellular matrices and forms morphogenic gradients, regulating cellular responses dependent on the local concentration of the signaling molecule.
Synonyms NOG; SYM1; SYNS1; SYNS1A; noggin

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.