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| Z03365 | |
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| $165.00 | |
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| Ask us a question | |
| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as analyzed by SDS-PAGE | ||||
| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method | ||||
| Biological Activity | Measured by its ability to inhibit the VEGF dependent proliferation of HUVEC (human umbilical vein endothelial cells). The ED50 for this effect is < 30.0 ng/ml in the presence of 2.5 ng/ml rhVEGF165 (Cat. No.: Z02689). | ||||
| Expression System | CHO | ||||
| Apparent Molecular Weight | ~80 kDa, on SDS-PAGE under reducing conditions. | ||||
| Formulation | Lyophilized after extensive dialysis against PBS. | ||||
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. | ||||
| Storage & Stability | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background | Vascular endothelial growth factor receptor 1 (VEGF R1) , also known as FMS-like tyrosine kinase (Flt1), is a receptor tyrosine kinase which plays a critical role in angiogenesis. Human VEGF R1 contains a signal peptide (aa 1-22), an extracellular domain (ECD aa 27-758) with seven Ig-like repeats, a trans-membrane domain (aa 759-780) and a cytoplasmic region (aa 781-1338) with a tyrosine kinase domain and several autocatalytic phosphotyrosine sites. VEGFR-1 and VEGFR-2 are closely related receptor tyrosine kinases and have both common and specific ligands. VEGFR-1 is a kinase-impaired RTK whereas VEGFR-2 is a highly active kinase. Vascular endothelial growth factors (VEGFs) are crucial regulators of vascular development during embryogenesis (vasculogenesis) as well as blood-vessel formation (angiogenesis) in the adult. In mammals, five VEGF ligands, which occur in several different splice variants and processed forms, have been identified so far. These ligands bind in an overlapping pattern to VEGF receptor-1, -2 and -3 (VEGFR1-3), as well as to co-receptors (here defined as VEGF-binding molecules that lack established VEGF-induced catalytic function), such as heparin sulphate proteoglycans (HSPGs) and neuropilins. |
| Synonyms | FLT1; Flt-1; VEGFR1; FLT; VEGFR-1; VEGFR1; fms related tyrosine kinase 1; vascular endothelial growth factor receptor 1; fms related receptor tyrosine kinase 1 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
