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TNF-α, Porcine

Recombinant Porcine Tumor Necrosis Factor-alpha (TNF-α) produced in E. coli is a single non-glycosylated polypeptide chain containing 155 amino acids. A fully biologically active molecule, rpTNF-α has a molecular mass of 17.2 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Z03338
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Product Introduction
Species Porcine
Protein Construction
TNF-α (Arg78Leu232)
Accession # P23563
Purity > 98% as analyzed by SDS-PAGE
> 98% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 20.0 ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of > 5.0 × 104 units/mg.
Expression System E. coli
Apparent Molecular Weight ~17.2 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Background
Target Background Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune disease. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune disease including Crohn's disease, rheumatoid arthritis and graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
Synonyms TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.