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CNTF, Mouse

Alternative product(s) for purchase:Z03593

Recombinant Mouse CNTF produced in E. coli is a single, non-glycosylated polypeptide chain of 197 amino acids and a molecular mass of 22.6 kDa. It has been purified by chromatographic techniques.
Z03312
$65.00

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Product Introduction
Species Mouse
Protein Construction
CNTF (Ala2-Met198)
Accession # P51642
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 30.0 ng/ml, measured by its ability to induce alkaline phosphatase production by TF-1 Cells.
Expression System E. coli
Apparent Molecular Weight ~22.6 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Background
Target Background Ciliary Neurotrophic Factor (CNTF) is a polypeptide hormone which acts within the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF is a potent survival factor for neurons and oligodendrocytes and may play a role in reducing tissue damage during increased inflammation. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, however this phenotype is not causally related to neurologic disease.
Synonyms Ciliary Neurotrophic Factor

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.