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EGF R, His, Human

Recombinant human Epidermal Growth Factor Receptor (rhEGFR) with C-terminal 6xHis-tag produced in CHO cells is a single glycosylated polypeptide chain containing 627 amino acids. rhEGFR has a molecular mass of 80 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
$90.00
Z03202-10

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Product Introduction
Species Human
Protein Construction
EGFR/ErbB1 (Leu25-Ser645)
Accession # P00533-1		 Poly-His
N-term C-term
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 1.0 μg/ml, measured in a bioassay using 3T3 cells in the presence of 25.0 pg/ml human EGF.
Expression System CHO
Apparent Molecular Weight 95~115 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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Background
Target Background EGF Receptor, also known as ERBB, ERBB1 and HER1, is a type I transmembrane protein belonging to the tyrosine protein kinase family. It belongs to a family of tyrosine kinase receptors including Human EGF Receptors (HER) 2, 3, and 4 which all play important roles in cell growth and differentiation. Their primary ligands are EGF, Heparin-Binding EGF and Transforming Growth Factor α. Upon ligand binding, EGFR undergoes asymmetric dimerization, composed of an “activator” and a “receiver”. EGFR and its family members are disregulated in numerous cancers. In particular, EGFR is overexpressed in many epithelial solid tumors. Evidence suggests EGFR is an excellent target for pharmacologic intervention in Non Small Cell Lung Cancer (NSCLC) due to its high level of expression and prominent role in tumor growth and metastasis.
Synonyms ERBB; ERBB1; HER1
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.