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FGF-10, Mouse

ED₅₀< 10 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 1.0× 10⁵ units/mg.

FGF-10, Mouse

Recombinant mouse Fibroblast Growth Factor-10 (rmFGF-10) produced in E. coli is a single non-glycosylated polypeptide chain containing 148 amino acids. A fully biologically active molecule, rmFGF-10 has a molecular mass of 17.0 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Z03155
$70.00

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Product Introduction
Species Mouse
Protein Construction
FGF-10 (Ser62-Thr209)
Accession # O35565
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 10.0 ng/ml, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 1.0 × 105 units/mg.
Expression System E. coli
Apparent Molecular Weight ~17 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • FGF-10, Mouse
    • FGF-10, Mouse

    ED₅₀< 10 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 1.0× 10⁵ units/mg.


Background
Target Background Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares homology with KGF, and both KGF and FGF-10 activate the receptor FGFR2-IIIb. However, unlike KGF, which induces the proliferation and differentiation of various epithelial cells, FGF-10 is an essential factor for the budding and branching morphogenesis during multi-organ development via mesenchymal-epithelial interactions. FGF-10 is crucial for lung and limb development and is regulated by Shh during early development.
Synonyms Fibroblast Growth Factor-10; FGFA; Keratinocyte growth factor-2

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.