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OSM (227aa), Human

ED₅₀< 10 ng/mL, measured by a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1× 10⁵ units/mg.

OSM (227aa), Human

Recombinant human Oncostatin M (rhOSM) produced in E. coli is a single non-glycosylated polypeptide chain containing 228 amino acids. A fully biologically active molecule, rhOSM has a molecular mass of 25.9 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Z03068
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Product Introduction
Species Human
Protein Construction

Expressed with an N-terminal Met.

OSM (Ala26-Arg252)
Accession # P13725
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 10.0 ng/ml, measured by a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1.0 × 105 units/mg.
Expression System E. coli
Apparent Molecular Weight ~25.9 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • OSM (227aa), Human
    • OSM (227aa), Human

    ED₅₀< 10 ng/mL, measured by a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1× 10⁵ units/mg.


Background
Target Background Oncostatin M (OSM) is a multifunctional cytokine, and belongs to Interleukin-6 (IL-6) subfamily, including IL-11, leukemia inhibitory factor (LIF), ciliary neurotropic factor, cardiotrophin-1, and novel neurotropin-1. In vivo, OSM is secreted from activated T cells, monocytes, neutrophils, and endothelial cells. OSM is related to LIF, and share a receptor with LIF in human. Human OSM can bind to gp130 and recruit OSM Receptor β or LIF Receptor β to form a ternary complex. OSM stimulates the growth of different types of cells, including megakaryocytes, fibroblasts, vascular endothelial cells, and T cells. On the other hand, OSM inhibits the proliferation of several cancer cell lines, such as solid tissue tumor cells, lung cancer cells, melanoma cells, and breast cancer cells.
Synonyms OncoM; oncostatin M

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.