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Shh, Mouse

ED₅₀<1.0μg/ml, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of>1.0× 10³units/mg.

Shh, Mouse

Recombinant mouseSonic Hedgehog(rmShh) produced in E.coli is a single non-glycosylated polypeptide chain containing 176 amino acids. A fully biologically active molecule, rmShh,is obtained by proprietary chromatographic techniques at GenScript with a molecular mass of 19.8 kDa analyzed by reducing SDS-PAGE.
Z02990
$70.00

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Product Introduction
Species Mouse
Protein Construction
Shh [Cys25-Gly198(Cys25Ile-Val-Ile)]
Accession # Q62226
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 1.0 μg/ml, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of > 1.0 × 103 units/mg.
Expression System E. coli
Apparent Molecular Weight ~19.8 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • Shh, Mouse
    • Shh, Mouse

    ED₅₀<1.0μg/ml, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of>1.0× 10³units/mg.


Background
Target Background Members of the Hedgehog (Hh) family are highly conserved proteins which are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). The biologically active form of Hh molecules is obtained by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal one half of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can substitute for each other in experimental systems.
Synonyms Hhg1; HHG-1; ShhNC; Sonic hedgehog protein N-product; Sonic hedgehog protein 19 kDa product

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.