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IFN-γ, Mouse

ED₅₀<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells.

IFN-γ, Mouse

Recombinant mouse IFN gamma (rmIFN-γ) produced in E. coli is a non-glycosylated polypeptide chain of 134 amino acids. A fully biologically active molecule, rmIFN--γ has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Z02916
$45.00

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Product Introduction
Species Mouse
Protein Construction

Expressed with an N-terminal Met.

IFN-γ (His23-Cys155)
Accession # P01580
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 1 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.15 ng/ml, measured by cytotoxicity assay using WEHI-279 cells.
Expression System E. coli
Apparent Molecular Weight ~15 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • IFN-γ, Mouse
    • IFN-γ, Mouse

    ED₅₀<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells.


Background
Target Background Sharing 41% sequence identity with human Interferon gamma (hIFN--γ), mouse IFN gamma (mIFN--γ)is a macrophage-activating factor.The active form of IFN--γ is an antiparallel dimer that sets off IFN--γ/JAK/STAT pathway. IFN--γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--γ–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--γ is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers.
Synonyms Immune Interferon; type II interferon; T cell interferon; MAF; IFNG; IFG; IFI; IFN-gamma

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.