Catalog Products » Recombinant Proteins » Cytokines » TNF-α, Human
Manual
Manual
SDS
COAs

TNF-α, Human

Recombinant Human Tumor Necrosis Factor-alpha (TNF-α) produced in E.coli is a single non-glycosylated polypeptide chain containing 157 amino acids. A fully biologically active molecule, rhTNF-α has a molecular mass of 17.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
$55.00
Z01001-10

Ask us a question
Product Introduction
Species Human
Protein Construction
TNF-α (Val77-Leu233)
Accession # P01375
Purity > 98% as analyzed by SDS-PAGE
> 98% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 30.0 pg/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of > 3.3 × 107 units/mg.
Expression System E. coli
Apparent Molecular Weight ~17.3 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
Back

TNF-α, Human

Biological Activity
TNF-alpha, Human (Cat. No. Z01001) stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED50 for this effect is typically 11-50 pg/mL. »

TNF-α, Human

2 μg of TNF-α, Human (Cat. No. Z01001) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining. »

TNF-α, Human

GenScript product showed better activity compared to competitor P »

TNF-α, Human

GenScript product showed better activity compared to competitor R »

<
>

Background
Target Background Tumor Necrosis Factor-alpha (TNF-a) is a homotrimer with a subunit molecular mass of 17.3 kDa. Tumor Necrosis Factor-alpha(TNF-a) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease.
Synonyms TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2
Back

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.