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MonoRab™ Anti-Camelid VHH Affinity Resin

Camelid VHH Antibody (also called single-domain antibody, sdAb or Nanobody by Ablynx) is a peptide chain about 110 amino acids in length, containing one variable domain of a heavy-chain antibody (a VHH fragment). Like a whole antibody, the camelid VHH antibody is able to bind selectively to a specific antigen. This single-domain antibody has a broad range of applications in biotechnical and therapeutic research fields due to its small size, simple production and high affinity. GenScript’s MonoRab™ Anti-Camelid VHH Affinity Resin (Cat. No. L00905) is designed for purification of Camelid VHH single domain antibody from commonly used protein expression systems such as bacteria, yeast and mammalian cells. The VHH single domain antibody in cell lysate can specifically bind to the Anti-Camelid VHH monoclonal antibody coupled to the resin. Nonspecific binding reactions are eliminated by stringent washing steps, and the purified target protein is eluted with a high recovery rate. Table 1 lists the main characteristics of GenScript’s MonoRab™ Anti-Camelid VHH Affinity Resin.
$474.99
L00905-1

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Description

Camelid VHH Antibody (also called single-domain antibody, sdAb or Nanobody by Ablynx) is a peptide chain about 110 amino acids in length, containing one variable domain of a heavy-chain antibody (a VHH fragment). Like a whole antibody, the camelid VHH antibody is able to bind selectively to a specific antigen. This single-domain antibody has a broad range of applications in biotechnical and therapeutic research fields due to its small size, simple production and high affinity.
GenScript’s MonoRab™ Anti-Camelid VHH Affinity Resin (Cat. No. L00905) is designed for purification of Camelid VHH single domain antibody from commonly used protein expression systems such as bacteria, yeast and mammalian cells. The VHH single domain antibody in cell lysate can specifically bind to the Anti-Camelid VHH monoclonal antibody coupled to the resin. Nonspecific binding reactions are eliminated by stringent washing steps, and the purified Camelid VHH single domain antibody is eluted with a high recovery rate. Table 1 lists the main characteristics of GenScript’s MonoRab™ Anti-Camelid VHH Affinity Resin.

 Table 1. Characteristics of Anti-Camelid VHH Affinity Resin
Product content50% settled resin in TBS with 0.02% sodium azide
Matrix4% cross-linked agarose
Average bead size90 μm
LigandMonoRab™ Rabbit Anti-Camelid VHH Antibody, mAb 
Binding capacityApproximately 2-4 mg Camelid VHH  protein per mL settled resin (depending on amino acid sequence of different Camelid VHHs)
Storage and stabilityStore at 2-8 °C for up to 12 months. Do not freeze the resin.
Resin reuseWhen stored at 2 - 8° C, the resin can be recycled at least 5 times with no loss of binding capability. The resin can be reused up to 10 times with minimum loss of binding capability.
Elution methodAcid buffer elution (pH 2.5)
Reagents compatibilityCompatible with commonly used bacterial lysis reagents. For reagents compatible with purification, see tables 2 and 3 in the manual. 
Note

Limited Use Label license: This product may be the subject of one or more patents filed by GenScript Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer should not sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. For commercial use, please contact GenScript at product@genscript.com.

For laboratory or further manufacturing use only.

MonoRab™ Anti-Camelid VHH Affinity Resin

Figure 1. Purification of Camelid VHH#1 from E. coli lysate

Lanes:
1. Protein marker
2. Whole cell lysate
3. Flow through
4. Washing eluate(first time)
5. Washing eluate(final-time)
6. Eluate of target Camelid VHH #1 (M.W.=15 kDa monomer and 30 kDa dimer) »

MonoRab™ Anti-Camelid VHH Affinity Resin

Figure 2. Purification of Camelid VHH#2 from E. coli lysate

Lanes:
1. Protein marker
2. Flow through 1
3. Flow through 2
4. Eluate 1 of target Camelid VHH #2 (M.W.=15 kDa)
5. Eluate 2 of target Camelid VHH #2 (M.W.=15 kDa)
6. Empty elution buffer
7. Whole cell lysate »

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